##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938204_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2212092 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.953448138685008 33.0 31.0 34.0 30.0 34.0 2 32.07468947946107 33.0 31.0 34.0 30.0 34.0 3 32.100106143867436 34.0 31.0 34.0 30.0 34.0 4 35.61456440328883 37.0 35.0 37.0 33.0 37.0 5 35.62224898421946 37.0 35.0 37.0 33.0 37.0 6 35.65243579380966 37.0 35.0 37.0 33.0 37.0 7 35.586689432446754 37.0 35.0 37.0 33.0 37.0 8 35.57311540388013 37.0 35.0 37.0 33.0 37.0 9 37.20813962529587 39.0 37.0 39.0 34.0 39.0 10-11 37.11178332546748 39.0 37.0 39.0 33.5 39.0 12-13 36.96411609462898 39.0 37.0 39.0 33.0 39.0 14-15 38.13046790097337 40.0 38.0 41.0 33.0 41.0 16-17 37.94527488006828 40.0 38.0 41.0 33.0 41.0 18-19 38.13675041544384 40.0 38.0 41.0 33.0 41.0 20-21 38.1355854548545 40.0 38.0 41.0 33.0 41.0 22-23 38.02642182151556 40.0 38.0 41.0 33.0 41.0 24-25 37.90189942371293 40.0 38.0 41.0 33.0 41.0 26-27 37.70828496283156 40.0 38.0 41.0 32.0 41.0 28-29 37.52234084296675 40.0 37.0 41.0 32.0 41.0 30-31 37.28227148780431 40.0 37.0 41.0 31.5 41.0 32-33 36.971081898944526 40.0 36.0 41.0 30.5 41.0 34-35 36.726479730499456 39.0 36.0 41.0 30.0 41.0 36-37 36.44410901535741 39.0 35.5 41.0 30.0 41.0 38-39 36.12341484893034 39.0 35.0 40.5 29.5 41.0 40-41 35.9784667635885 39.0 35.0 40.0 29.0 41.0 42-43 35.743668436936616 39.0 35.0 40.0 27.5 41.0 44-45 35.394579655819015 38.5 35.0 40.0 26.5 41.0 46-47 34.99009715689944 38.0 34.5 40.0 24.5 41.0 48-49 34.558011827717834 38.0 34.0 40.0 23.5 41.0 50-51 33.81153970992165 37.5 33.5 39.5 22.5 40.5 52-53 33.86116445428129 38.0 33.0 39.5 22.5 40.5 54-55 33.91298598792456 38.0 33.0 40.0 21.5 41.0 56-57 33.57299809411182 38.0 33.0 40.0 19.5 41.0 58-59 33.13838506716719 37.0 32.5 40.0 16.5 41.0 60-61 32.60902824114007 36.5 32.0 40.0 10.5 41.0 62-63 32.033024168976695 36.0 31.0 39.0 7.5 41.0 64-65 31.467248423664117 35.5 30.5 39.0 4.5 40.0 66-67 30.866012805977327 35.0 29.5 38.5 2.0 40.0 68-69 30.14755602389051 35.0 29.0 38.0 2.0 40.0 70-71 29.52563455769471 34.0 28.0 37.0 2.0 39.5 72-73 28.813090956434 34.0 26.5 36.5 2.0 39.0 74-75 28.119200964516846 34.0 26.0 36.0 2.0 39.0 76-77 27.34262883279719 33.0 25.5 35.0 2.0 38.0 78-79 26.553807662610776 32.5 23.0 35.0 2.0 37.0 80-81 25.814188559969477 32.0 20.5 35.0 2.0 36.5 82-83 25.04143362934272 31.5 18.0 35.0 2.0 36.0 84-85 24.291543028047656 31.0 10.5 34.0 2.0 35.5 86-87 23.523847109433063 30.5 4.5 34.0 2.0 35.0 88-89 22.61197522526188 29.5 2.0 34.0 2.0 35.0 90-91 21.679103310350563 29.0 2.0 34.0 2.0 35.0 92-93 20.718302403335848 28.5 2.0 34.0 2.0 35.0 94-95 19.599936621080857 26.5 2.0 33.5 2.0 35.0 96-97 18.30410850904935 24.5 2.0 33.0 2.0 35.0 98-99 16.726940380418174 11.0 2.0 32.0 2.0 34.0 100-101 12.887467609846244 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19933292010163584 1101 2 0.14817283392783054 1101 3 0.2152114458417529 1101 4 0.03114146257350825 1101 5 0.03486586844253736 1101 6 0.05672372810559523 1101 7 0.07887728493757606 1101 8 0.19973634900217263 1101 9 0.07312658102974723 1101 10-11 0.2079915590061603 1101 12-13 0.2042803429464186 1101 14-15 0.18257071376824285 1101 16-17 0.2138766905908085 1101 18-19 0.26601196817403405 1101 20-21 0.27860710183043125 1101 22-23 0.12865894071930484 1101 24-25 0.2113625895330813 1101 26-27 0.2409431059457603 1101 28-29 0.2234941945212725 1101 30-31 0.2731874002250194 1101 32-33 0.16800653142463773 1101 34-35 0.10847532752884348 1101 36-37 0.02106200056914531 1101 38-39 0.24823158215343 1101 40-41 0.32764794824685595 1101 42-43 0.4697558492893066 1101 44-45 0.5218794482132836 1101 46-47 0.37746273313985057 1101 48-49 0.45711335035421286 1101 50-51 0.42455020988195 1101 52-53 0.3914646244735067 1101 54-55 0.36486846041017174 1101 56-57 0.2579131072104275 1101 58-59 0.3587091989859843 1101 60-61 0.2900521122030142 1101 62-63 0.11014994010255208 1101 64-65 -0.038360527965483726 1101 66-67 0.00268380130273016 1101 68-69 0.08610757364401067 1101 70-71 0.1637309071102102 1101 72-73 -0.09278935666696952 1101 74-75 -0.269805788169581 1101 76-77 -0.3128781816367159 1101 78-79 -0.3462176326731665 1101 80-81 -0.6733695615096451 1101 82-83 -0.5889804084476005 1101 84-85 -0.5941859174822355 1101 86-87 -0.3811767547406433 1101 88-89 -0.44360405913355905 1101 90-91 -0.9582889918419326 1101 92-93 -1.006658162115631 1101 94-95 -1.033399814154759 1101 96-97 -1.1266938761677636 1101 98-99 -1.2263949766895976 1101 100-101 -1.3446819243098904 1102 1 0.20274063907192286 1102 2 0.20004643027215963 1102 3 0.21882362795025045 1102 4 0.13106065095221453 1102 5 0.1676159950149554 1102 6 0.07258584855060946 1102 7 0.20424402040770673 1102 8 0.29173989302989867 1102 9 0.4010367730303699 1102 10-11 0.3356333525697863 1102 12-13 0.3001062117524853 1102 14-15 0.2804395912331614 1102 16-17 0.3408750743583866 1102 18-19 0.4596693918086743 1102 20-21 0.28890977218392777 1102 22-23 0.3892399081220361 1102 24-25 0.43789557625270703 1102 26-27 0.29478749976124163 1102 28-29 0.3136105113287897 1102 30-31 0.43265404352447234 1102 32-33 0.3651925669168392 1102 34-35 0.27234198510824825 1102 36-37 0.3381681007106465 1102 38-39 0.23743641527256187 1102 40-41 0.16692612846001964 1102 42-43 0.337434121673148 1102 44-45 0.1884479401191399 1102 46-47 0.12635603583616728 1102 48-49 -0.13151736325458785 1102 50-51 0.043287309231565985 1102 52-53 -0.005461613015299349 1102 54-55 -0.2637550807615625 1102 56-57 -0.4926288823781846 1102 58-59 -0.41546532166155004 1102 60-61 -0.1998991736429403 1102 62-63 -0.3067731168485466 1102 64-65 -0.5836036768276287 1102 66-67 -0.5301917617822625 1102 68-69 -0.5214684347162617 1102 70-71 -0.6594815008813733 1102 72-73 -0.6439870887488617 1102 74-75 -0.837163410425557 1102 76-77 -0.8090761408998688 1102 78-79 -0.8861382490483898 1102 80-81 -0.8011857978300867 1102 82-83 -0.8176951466698767 1102 84-85 -0.7745280849213181 1102 86-87 -0.8849341705862095 1102 88-89 -0.7343952665695248 1102 90-91 -0.9763020320465223 1102 92-93 -1.0830235215163917 1102 94-95 -0.9865079784003221 1102 96-97 -1.1916287725081993 1102 98-99 -1.2441126812500993 1102 100-101 -1.0956474446848024 1103 1 0.20708653953142786 1103 2 0.18434905626637033 1103 3 0.20975813495351758 1103 4 0.05037776328128274 1103 5 0.22677698709821925 1103 6 0.18606598289522225 1103 7 0.06407027665897402 1103 8 0.08371104953767627 1103 9 0.2034920683855148 1103 10-11 0.1796353640481385 1103 12-13 0.1195587297912013 1103 14-15 0.11724572347402074 1103 16-17 0.07945324520486707 1103 18-19 0.15760393571979847 1103 20-21 0.11409338878507924 1103 22-23 0.08377777558591504 1103 24-25 0.12190086330358696 1103 26-27 -0.0013669319499953758 1103 28-29 0.07973001897794774 1103 30-31 0.007620826450896345 1103 32-33 -0.11945030340739038 1103 34-35 -0.20834482402275256 1103 36-37 -0.23123712913304928 1103 38-39 -0.3162749006771648 1103 40-41 -0.054194624829392524 1103 42-43 0.07534449093628126 1103 44-45 0.1443372811293031 1103 46-47 0.05814108446227095 1103 48-49 0.006193934241210286 1103 50-51 0.16212485006088428 1103 52-53 0.16148878282149326 1103 54-55 0.25544623315823856 1103 56-57 0.1524651403939714 1103 58-59 0.30826666268952607 1103 60-61 0.017175555545634325 1103 62-63 -0.203500312404838 1103 64-65 -0.2770908236655494 1103 66-67 -0.30425453282435555 1103 68-69 -0.18286073758215338 1103 70-71 -0.5104406438808802 1103 72-73 -0.4326353845284423 1103 74-75 -0.4968152770405503 1103 76-77 -0.32609297088380984 1103 78-79 -0.6739526578767503 1103 80-81 -0.7079669249961142 1103 82-83 -0.7454595453485737 1103 84-85 -0.8844016293676056 1103 86-87 -1.0023830532007665 1103 88-89 -0.8311203860587177 1103 90-91 -0.9255502650827516 1103 92-93 -1.069381453938103 1103 94-95 -1.0688286523378352 1103 96-97 -1.254911911152572 1103 98-99 -1.2876097470752672 1103 100-101 -1.3273388396173544 1104 1 0.22916782714716177 1104 2 0.3196295812284191 1104 3 0.3044534134748105 1104 4 0.22261369420106547 1104 5 0.23767352623890758 1104 6 0.2700525060234753 1104 7 0.2975563778787702 1104 8 0.28040678154704324 1104 9 0.35147267922958747 1104 10-11 0.32907202490464016 1104 12-13 0.24254813089897453 1104 14-15 0.18415336315089093 1104 16-17 0.1959868540188907 1104 18-19 0.39087950619158107 1104 20-21 0.24944312270567792 1104 22-23 0.41531445887483187 1104 24-25 0.3645886101478695 1104 26-27 0.3150167402531494 1104 28-29 0.23895732757464572 1104 30-31 0.2780729811604985 1104 32-33 0.30786008401032205 1104 34-35 0.23955970536480464 1104 36-37 0.2863470281135747 1104 38-39 0.16968245644081748 1104 40-41 0.14207375396043176 1104 42-43 0.10150438831343678 1104 44-45 0.1800414810725357 1104 46-47 0.061119005594548526 1104 48-49 -0.09106425365046533 1104 50-51 -0.15042825148051264 1104 52-53 0.020687512474125924 1104 54-55 -0.16645665989722147 1104 56-57 -0.09177605718236492 1104 58-59 -0.16986173347998346 1104 60-61 -0.13139671929717878 1104 62-63 -0.04023226538243563 1104 64-65 -0.358997811181073 1104 66-67 -0.16902906044296273 1104 68-69 -0.10115460124001885 1104 70-71 -0.22247729171835218 1104 72-73 -0.3592786072653773 1104 74-75 -0.3772271574600552 1104 76-77 -0.5952804891691343 1104 78-79 -0.7160526367510798 1104 80-81 -0.5976166284502469 1104 82-83 -0.6633064546482963 1104 84-85 -0.7311556929135286 1104 86-87 -0.758657655957073 1104 88-89 -0.6796155630896692 1104 90-91 -0.7065859456887296 1104 92-93 -0.6351858640974939 1104 94-95 -1.168188275879821 1104 96-97 -1.538210676305038 1104 98-99 -1.9116453892049385 1104 100-101 -1.7238382940523298 1105 1 0.05227298220900778 1105 2 0.19457671290752643 1105 3 0.18656859175667506 1105 4 0.18541962308922422 1105 5 0.07777836062498977 1105 6 0.0811622055450556 1105 7 0.20322315734634344 1105 8 0.26737566372494115 1105 9 0.17271536942429577 1105 10-11 0.19331117675699971 1105 12-13 0.22254793999348266 1105 14-15 0.17008010589642453 1105 16-17 0.21649430808282233 1105 18-19 0.1820709450803477 1105 20-21 0.1317409111792145 1105 22-23 0.0844025192591431 1105 24-25 0.19781811968211827 1105 26-27 0.1664313612955297 1105 28-29 0.12120057162253772 1105 30-31 -0.023192244972726428 1105 32-33 -0.004064145600800373 1105 34-35 -0.06382513987561822 1105 36-37 0.13002372693948416 1105 38-39 -0.03634252081528899 1105 40-41 -0.09336566843228411 1105 42-43 -0.025774801647301615 1105 44-45 -0.024573636415020417 1105 46-47 -0.11414302923524389 1105 48-49 -0.08511936579266433 1105 50-51 -0.299324420030068 1105 52-53 -0.05740430988468859 1105 54-55 -0.2123802254299605 1105 56-57 -0.3788785959299261 1105 58-59 -0.29031355222976885 1105 60-61 -0.305368096655279 1105 62-63 -0.31701352155419116 1105 64-65 -0.3907390306080707 1105 66-67 -0.45267609619206084 1105 68-69 -0.28939684576131697 1105 70-71 -0.33213397319458693 1105 72-73 -0.478328407007794 1105 74-75 -0.6983455318179956 1105 76-77 -0.9007971123782887 1105 78-79 -0.9074386856051575 1105 80-81 -0.8354689598721805 1105 82-83 -0.8258839560272513 1105 84-85 -0.8399077323636952 1105 86-87 -0.7262592898353404 1105 88-89 -0.7973716372364557 1105 90-91 -0.6823968565283671 1105 92-93 -0.7828714873005111 1105 94-95 -0.9869164680525593 1105 96-97 -0.900223279249122 1105 98-99 -0.8735823572121326 1105 100-101 -0.908635489923407 1106 1 0.22951939990790393 1106 2 0.18915565612446272 1106 3 0.1890222823095442 1106 4 0.12672147033760695 1106 5 0.15674649918337735 1106 6 0.18496461534452635 1106 7 0.3381575070300329 1106 8 0.049139959418248225 1106 9 0.15970555901731842 1106 10-11 0.22353226780123236 1106 12-13 0.3432365605891974 1106 14-15 0.2953671279276122 1106 16-17 -0.2466625882611524 1106 18-19 0.13584799773851586 1106 20-21 0.19040978707874956 1106 22-23 0.26585573025234055 1106 24-25 0.14580839686373537 1106 26-27 0.06747523834436464 1106 28-29 0.031146184425765 1106 30-31 0.030919967152335914 1106 32-33 0.10274023034495627 1106 34-35 0.03549331436466474 1106 36-37 0.10731964913370717 1106 38-39 0.11058759558076048 1106 40-41 0.017851043665928046 1106 42-43 -0.22198185238432444 1106 44-45 0.03622047279198881 1106 46-47 0.146165084227313 1106 48-49 0.1787297950262854 1106 50-51 0.09820212149298158 1106 52-53 0.1239461901687875 1106 54-55 0.029190944661770857 1106 56-57 -0.05787351917859951 1106 58-59 0.10850177567824204 1106 60-61 0.34520686684543733 1106 62-63 0.1184974369409062 1106 64-65 -0.03543648458655113 1106 66-67 -0.14327723465028086 1106 68-69 -0.5291985809834046 1106 70-71 -0.6123856718569023 1106 72-73 -0.3892869746970824 1106 74-75 -0.38915112432583143 1106 76-77 -0.9934745603465451 1106 78-79 -0.9568615202916 1106 80-81 -0.7255214356050566 1106 82-83 -0.6902002641049023 1106 84-85 -0.738763225209258 1106 86-87 -0.9531192046953514 1106 88-89 -1.162008296984748 1106 90-91 -1.074671953444426 1106 92-93 -1.0364697575950537 1106 94-95 -0.9668335153586298 1106 96-97 -1.0623129980413246 1106 98-99 -1.2451715434559798 1106 100-101 -1.3101748958404293 1107 1 0.09409076674046091 1107 2 0.1284969905220592 1107 3 0.1378409750770544 1107 4 0.14765690249871 1107 5 0.13182339715768165 1107 6 0.1466346960240017 1107 7 0.2032435114884592 1107 8 0.21056705352372518 1107 9 0.19368332861224502 1107 10-11 0.23890425555048012 1107 12-13 0.2833236124035494 1107 14-15 0.26904034846990754 1107 16-17 0.23323898233174134 1107 18-19 0.2994135721415745 1107 20-21 0.2584230962163261 1107 22-23 0.35493820236510487 1107 24-25 0.3152959421481043 1107 26-27 0.2554863204724924 1107 28-29 0.20809619578130878 1107 30-31 0.03900522412938301 1107 32-33 0.20268554734099808 1107 34-35 0.2813269325053014 1107 36-37 0.2959857145813274 1107 38-39 0.2754352438808425 1107 40-41 0.26638441463057916 1107 42-43 0.3360528007644703 1107 44-45 0.3315855369673457 1107 46-47 0.13675729234024203 1107 48-49 0.24727924014814562 1107 50-51 0.17244278844923855 1107 52-53 0.17026754542530398 1107 54-55 0.34412965780311566 1107 56-57 0.28975636949297723 1107 58-59 0.12957523832511697 1107 60-61 0.2376800159470278 1107 62-63 0.20272480798934822 1107 64-65 0.14361187679754295 1107 66-67 0.20855574846193292 1107 68-69 0.1697039002579004 1107 70-71 0.1689655495806015 1107 72-73 0.039867996495114966 1107 74-75 0.0011623768637463172 1107 76-77 0.012426794160280252 1107 78-79 -0.4608162260066315 1107 80-81 -0.13296769522143848 1107 82-83 -0.23490692727303397 1107 84-85 -0.4619210363897288 1107 86-87 -0.3638334352856418 1107 88-89 -0.3190032013225661 1107 90-91 -0.3097681889164967 1107 92-93 -0.5517666664618623 1107 94-95 -0.3825939067831001 1107 96-97 -0.41281341367625757 1107 98-99 -0.45958459417226294 1107 100-101 -0.5236290095237486 1108 1 -0.029125829228654254 1108 2 -0.02179624279789749 1108 3 0.04984573532895098 1108 4 0.0032391543322702887 1108 5 -0.0226125175003844 1108 6 -0.09233445700277088 1108 7 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54-55 -0.037657028297267914 2316 56-57 -0.14443210922873106 2316 58-59 0.030722481965042903 2316 60-61 0.007998902537217134 2316 62-63 0.13040703579816437 2316 64-65 0.22306141623853648 2316 66-67 0.25710266334642284 2316 68-69 0.2461768660353414 2316 70-71 0.26819458948952857 2316 72-73 0.42684050206131374 2316 74-75 0.6489204796342101 2316 76-77 0.5514034791091724 2316 78-79 0.7192342542591526 2316 80-81 0.6950837062396467 2316 82-83 0.4239246848728335 2316 84-85 0.2658695400605957 2316 86-87 0.5035737168066419 2316 88-89 0.6376755805048475 2316 90-91 0.6100210490185098 2316 92-93 0.714602131602394 2316 94-95 0.5951851933667491 2316 96-97 0.7330244760446085 2316 98-99 0.7176121899905965 2316 100-101 0.5900290245434743 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6876.0 3 2131.0 4 2027.0 5 2363.0 6 2769.0 7 3329.0 8 4415.0 9 5553.0 10 6980.0 11 8962.0 12 11164.0 13 14023.0 14 16375.0 15 20168.0 16 23609.0 17 29409.0 18 29067.0 19 25693.0 20 26060.0 21 27215.0 22 29802.0 23 33617.0 24 37408.0 25 41866.0 26 46319.0 27 53314.0 28 60953.0 29 70079.0 30 81816.0 31 96921.0 32 116499.0 33 142846.0 34 181577.0 35 233425.0 36 285229.0 37 288454.0 38 136223.0 39 7556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88963891160084 18.371693146844787 14.704851726395338 23.03381621515903 2 15.668649223184605 19.504421176417377 40.100318867455165 24.726610732942852 3 19.507706432983642 23.454865166850478 29.424792783787062 27.612635616378817 4 12.872504771944723 16.497960635812692 36.73960639433803 33.88992819790456 5 13.786075422492502 36.98993029151111 34.16336452272263 15.06062976327376 6 32.6010622829753 35.820297884946214 16.97840918458956 14.600230647488925 7 29.008014489472494 32.68775186778357 19.872809599275527 18.431424043468418 8 26.79525033282864 34.33036415049871 19.37128296111865 19.503102555553998 9 26.27100846991988 16.812569190926677 20.074805405469014 36.84161693368443 10-11 25.118650050229423 26.377440199831664 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36-37 22.54731224976325 25.80590428643757 29.073613322492477 22.573170141306704 38-39 21.960390106007065 26.342942756183746 28.9885851590106 22.708081978798585 40-41 22.64500685727106 26.015224408266356 29.09930016030454 22.24046857415804 42-43 22.138212748325834 26.122531046878418 28.94910933246243 22.790146872333317 44-45 22.202351651517272 26.454937683563788 29.08462142191231 22.25808924300663 46-47 22.722235916408675 26.677513969954703 28.217273218618764 22.382976895017855 48-49 22.4017622966296 26.721012587929977 28.121967858708746 22.755257256731678 50-51 22.60726915991582 27.087591481878658 28.015447609338402 22.289691748867117 52-53 22.94092138813689 26.64931352364967 28.060000605920564 22.349764482292876 54-55 22.353422727575964 26.928110339587313 28.307852695113166 22.410614237723557 56-57 22.502920517827537 27.063947527219547 27.951803033992405 22.48132892096051 58-59 22.519639917693148 26.59443521908354 27.89896130451704 22.986963558706275 60-61 22.117476169845983 26.971827100576412 28.187031548329156 22.723665181248457 62-63 22.298658898167577 26.911001702638266 28.399933875490984 22.390405523703173 64-65 21.95969283360569 26.974176758834222 28.200793715936882 22.8653366916232 66-67 21.920799762668818 27.01437554041685 28.13934704982146 22.925477647092873 68-69 22.574608467824746 26.493684731443896 28.364222856920556 22.5674839438108 70-71 22.261803425352092 26.80910622969742 27.840891101737462 23.088199243213026 72-73 22.254101270895326 26.88536794633646 27.875842224263568 22.98468855850465 74-75 22.20917703378495 27.265896091003754 27.79641968371197 22.728507191499318 76-77 22.41533628667706 26.777211701390495 27.93688329921426 22.870568712718182 78-79 22.440888807702166 26.968259257735333 27.849710418120154 22.741141516442344 80-81 22.693842023456266 27.061862371061185 27.443312137911548 22.800983467571005 82-83 22.53872820556413 27.183671236295748 27.595426180949715 22.68217437719041 84-85 22.519358105106523 27.231226351303235 27.530638040341 22.71877750324924 86-87 22.76663830048916 27.296439517190173 27.073406301632247 22.86351588068842 88-89 22.679292447239785 27.26384675721571 27.0547917320694 23.0020690634751 90-91 22.576585305586587 27.279151495705367 27.070138999984838 23.074124198723204 92-93 22.68997990121396 26.80528834462688 27.353282820571113 23.151448933588046 94-95 22.515392475031266 26.669771664997672 27.472494412247116 23.342341447723943 96-97 22.873319383943937 26.20968280029431 27.44784755161527 23.46915026414649 98-99 22.55494867717676 26.526776815431653 27.073170897187676 23.84510361020391 100-101 23.416194249150372 25.280078254430105 27.333012252376875 23.97071524404265 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 626.0 1 443.0 2 367.5 3 506.5 4 438.5 5 401.5 6 779.0 7 1710.5 8 2850.0 9 3375.5 10 2747.0 11 2013.0 12 1928.0 13 2023.0 14 2230.5 15 2434.0 16 2634.0 17 2887.0 18 3159.0 19 3570.5 20 4108.5 21 4835.5 22 5859.0 23 7096.0 24 8807.0 25 10967.5 26 13805.0 27 17205.0 28 20070.0 29 22782.0 30 25975.5 31 30931.0 32 38098.0 33 41669.5 34 42287.5 35 44743.5 36 48685.5 37 52858.5 38 55613.0 39 57819.0 40 60619.5 41 64182.5 42 66454.5 43 68689.0 44 71485.0 45 74765.0 46 76636.5 47 78085.5 48 79077.0 49 77787.5 50 77088.0 51 76389.5 52 75586.5 53 75910.0 54 77593.5 55 75127.0 56 69624.0 57 64426.0 58 60071.5 59 53386.5 60 42862.5 61 35410.0 62 30214.5 63 22946.0 64 16525.0 65 12776.0 66 9578.0 67 7138.5 68 5472.5 69 4116.0 70 3122.5 71 2257.0 72 1556.0 73 1159.5 74 866.5 75 658.0 76 501.5 77 331.0 78 197.0 79 126.5 80 90.5 81 57.0 82 31.5 83 21.0 84 14.0 85 10.5 86 9.5 87 8.5 88 6.5 89 5.0 90 2.5 91 1.5 92 2.0 93 1.5 94 0.0 95 0.0 96 0.5 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14782386989329557 2 0.05162533927160353 3 0.148637579268855 4 0.1277071658864098 5 0.18909701766472642 6 0.1986354997893397 7 0.1623802265005253 8 0.06758308424785225 9 0.11871115667883615 10-11 0.10089996256936871 12-13 0.14814031242823536 14-15 0.134488077349405 16-17 0.13396820747057536 18-19 0.130261309204138 20-21 0.1322277735284066 22-23 0.1926456946636939 24-25 0.21337268070224924 26-27 0.14725879393804597 28-29 0.12727770816042008 30-31 0.09958898635318963 32-33 0.1235934129321927 34-35 0.09326013565439412 36-37 0.08747375787263821 38-39 0.05219041522685314 40-41 0.05876789934595849 42-43 0.06159327912220649 44-45 0.03786008900172325 46-47 0.04959106583270497 48-49 0.04007518674630169 50-51 0.05130889673666375 52-53 0.02626473040000145 54-55 0.049704081023754884 56-57 0.02637774559105137 58-59 0.04961366887091495 60-61 0.03697857051153388 62-63 0.05024655394079451 64-65 0.03831214976592293 66-67 0.03779227988709331 68-69 0.06410221636351472 70-71 0.052710285105682764 72-73 0.05112807243098388 74-75 0.04405332147125888 76-77 0.02145028326127485 78-79 0.02056876477108547 80-81 0.02393661746437309 82-83 0.02084000122960528 84-85 0.01905436121101654 86-87 0.024320869113942822 88-89 0.06464468928055433 90-91 0.10919527759243287 92-93 0.07954009146093381 94-95 0.07583319319449644 96-97 0.0669728022161827 98-99 0.05576169526403061 100-101 0.1305551487008678 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2212092.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.461380672597905 #Duplication Level Percentage of deduplicated Percentage of total 1 80.74336283454826 48.01111834290768 2 11.54798138631606 13.733178344236283 3 3.411557131560504 6.0856769185810595 4 1.3899581758034831 3.3059532884176264 5 0.7366440177000628 2.190093517832769 6 0.458070557223623 1.6342504666789734 7 0.3086735008756264 1.2847906777376372 8 0.2209649545277911 1.051110502118422 9 0.16971783867075785 0.908249131091924 >10 0.9058284279059273 9.833465224657411 >50 0.0652505326854634 2.639474800262212 >100 0.03619175283876492 4.15253092234193 >500 0.002899444671814595 1.2783375819553626 >1k 0.0027468423206664233 3.05456260798999 >5k 1.5260235114813463E-4 0.8372076731907331 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9986 0.45142787912980115 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8467 0.3827598490478696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4946 0.22358925397316207 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3454 0.1561417879545697 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3106 0.1404100733604208 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2983 0.13484972596076475 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2820 0.12748113550430995 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2813 0.12716469296937016 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2450 0.11075488722892177 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2441 0.11034803254114205 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2391 0.10808772872014365 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2257 0.10203011447986793 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2255 0.10193970232702798 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.5206076419968065E-5 7 0.0 0.0 0.0 0.0 4.5206076419968065E-5 8 0.0 0.0 0.0 0.0 4.5206076419968065E-5 9 0.0 0.0 0.0 4.5206076419968065E-5 4.5206076419968065E-5 10-11 1.356182292599042E-4 0.0 0.0 4.5206076419968065E-5 4.5206076419968065E-5 12-13 1.356182292599042E-4 0.0 0.0 4.5206076419968065E-5 1.8082430567987226E-4 14-15 1.356182292599042E-4 0.0 0.0 4.5206076419968065E-5 2.2603038209984032E-4 16-17 1.5822126746988823E-4 0.0 0.0 9.041215283993613E-5 2.2603038209984032E-4 18-19 1.8082430567987226E-4 0.0 0.0 9.041215283993613E-5 2.712364585198084E-4 20-21 1.8082430567987226E-4 0.0 0.0 1.356182292599042E-4 3.3904557314976046E-4 22-23 1.8082430567987226E-4 0.0 0.0 1.8082430567987226E-4 3.842516495697286E-4 24-25 2.2603038209984032E-4 0.0 0.0 3.1644253493977645E-4 4.068546877797126E-4 26-27 2.712364585198084E-4 0.0 0.0 6.55488108089537E-4 4.5206076419968065E-4 28-29 2.712364585198084E-4 0.0 0.0 0.0025315402795182116 4.972668406196488E-4 30-31 2.712364585198084E-4 0.0 0.0 0.00924464262788347 4.972668406196488E-4 32-33 2.712364585198084E-4 0.0 0.0 0.02375579315869322 4.972668406196488E-4 34-35 2.712364585198084E-4 0.0 0.0 0.046313625292257285 5.424729170396168E-4 36-37 2.712364585198084E-4 0.0 0.0 0.08627579684750905 5.650759552496008E-4 38-39 2.712364585198084E-4 0.0 0.0 0.145812199492607 5.876789934595849E-4 40-41 2.712364585198084E-4 0.0 0.0 0.20749589076765343 5.876789934595849E-4 42-43 2.712364585198084E-4 0.0 0.0 0.270400146106039 5.876789934595849E-4 44-45 2.712364585198084E-4 0.0 0.0 0.3472504760199847 6.102820316695688E-4 46-47 2.712364585198084E-4 0.0 0.0 0.43411395186095336 6.55488108089537E-4 48-49 2.712364585198084E-4 0.0 0.0 0.5247521350829893 7.23297222719489E-4 50-51 2.712364585198084E-4 0.0 0.0 0.6230527482582098 7.23297222719489E-4 52-53 2.712364585198084E-4 0.0 0.0 0.7162224717597641 7.23297222719489E-4 54-55 2.712364585198084E-4 0.0 0.0 0.8175518920551225 7.23297222719489E-4 56-57 2.712364585198084E-4 0.0 0.0 0.9325109443911013 7.23297222719489E-4 58-59 2.712364585198084E-4 0.0 0.0 1.0555392813680444 7.23297222719489E-4 60-61 2.712364585198084E-4 0.0 0.0 1.1858231936103922 7.685032991394572E-4 62-63 2.712364585198084E-4 0.0 0.0 1.3135981686114322 7.685032991394572E-4 64-65 2.712364585198084E-4 0.0 0.0 1.4470691092413879 7.685032991394572E-4 66-67 2.712364585198084E-4 0.0 0.0 1.6038437822658371 7.685032991394572E-4 68-69 3.1644253493977645E-4 0.0 0.0 1.778045397750184 7.685032991394572E-4 70-71 3.1644253493977645E-4 0.0 0.0 1.9567676208765277 7.911063373494411E-4 72-73 3.1644253493977645E-4 2.2603038209984032E-5 0.0 2.138654269352269 9.267245666093452E-4 74-75 3.1644253493977645E-4 4.5206076419968065E-5 0.0 2.319659399337821 0.0010397397576592654 76-77 3.1644253493977645E-4 4.5206076419968065E-5 0.0 2.517390777598762 0.0010397397576592654 78-79 3.1644253493977645E-4 4.5206076419968065E-5 0.0 2.740030703967104 0.0010623427958692494 80-81 3.1644253493977645E-4 4.5206076419968065E-5 0.0 2.961743905768838 0.0011075488722892177 82-83 3.1644253493977645E-4 4.5206076419968065E-5 0.0 3.1928147653895045 0.0011301519104992017 84-85 3.1644253493977645E-4 4.5206076419968065E-5 0.0 3.4282254083464885 0.0011301519104992017 86-87 3.1644253493977645E-4 4.5206076419968065E-5 0.0 3.671140259989187 0.0011753579869191698 88-89 3.1644253493977645E-4 4.5206076419968065E-5 0.0 3.930012856608134 0.0012205640633391377 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4595 0.0 55.335148 1 GTATCAA 8110 0.0 42.389835 1 TGGTATC 1820 0.0 36.32587 2 GTGGTAT 1895 0.0 36.182507 1 ATCAACG 10415 0.0 32.927475 3 TATCAAC 10480 0.0 32.72251 2 TCAACGC 10560 0.0 32.475346 4 CAACGCA 10790 0.0 31.827183 5 AACGCAG 11160 0.0 30.767797 6 ACGCAGA 12615 0.0 26.947239 7 CGCAGAG 12545 0.0 26.791983 8 GCAGAGT 13555 0.0 24.12877 9 TAAGGTA 815 0.0 23.344515 4 GTGTAAG 1010 0.0 22.628992 1 GAGTACT 9060 0.0 22.612375 12-13 GTAAGGT 1605 0.0 22.52273 4 TAAGGTG 1795 0.0 21.99359 5 GGACCGT 195 4.1757448E-7 21.949839 6 CAGAGTA 12970 0.0 21.84181 10-11 AGAGTAC 12445 0.0 21.369164 10-11 >>END_MODULE