FastQCFastQC Report
Thu 26 May 2016
SRR938204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212092
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98300.4443757312082861No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84360.3813584606788506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49090.2219166291456232No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34520.15605137580172979No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31910.1442525898561181No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31540.14257996502857928No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30990.14009363082548104No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28480.12874690564406904No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA25130.11360287004337974No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24570.11107132976386153No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24480.11066447507608182No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24170.10926308670706282No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA22390.1012164051043085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45600.057.0882531
GTATCAA82400.043.5262031
TGGTATC16800.034.496242
TATCAAC105500.033.8149452
ATCAACG105500.033.7645763
TCAACGC106600.033.4123844
CAACGCA109250.032.645395
AACGCAG112850.031.646066
GTGGTAT19300.031.0130331
ACGCAGA128000.027.7891487
CGCAGAG128350.027.4913678
GTGTAAG9750.025.8227391
GCAGAGT141650.024.7424769
TAAGGTG15450.024.2829825
GAGTACT92800.022.95393412-13
CAGAGTA134600.022.00032810-11
GTACTTT96150.021.95660214-15
AGAGTAC129200.021.35754810-11
GTAAGGT14950.020.9655464
AGTACTT96700.020.82485812-13
Produced by FastQC (version 0.11.5)