FastQCFastQC Report
Thu 26 May 2016
SRR938203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271835
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60142.212371475343499No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51501.8945316092482571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43211.589567200691596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29671.091470929056229No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22150.8148325270844445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12720.46793091397355013No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.3586734600033108No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.32814023212610594No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.30349292769510916No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7410.2725918296025162No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5240.19276399286331783No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.18467084812478157No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA4700.17289900123236523No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4360.16039141390917286No Hit
GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3900.14346938400132433No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.14163003292438428No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT3460.12728309452425182No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3350.12323652215498374No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT3280.12066143064726764No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2980.1096253241856273No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.10521088160097118No Hit
GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.10484301138558316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA951.8095307E-840.2672041
GGTCTGG500.001607737938.0283666
GGTATCA21750.037.5942961
GTATCAA33700.034.7633251
ATTCCGT404.4854626E-429.69325326-27
CCGTAAC404.4854626E-429.69325328-29
TGCGAAT404.4878814E-429.6905218-19
TTTTCGA804.4844561E-429.6877887
GATGCGA404.4927225E-429.68505716-17
ATGCGAA404.4927225E-429.68505716-17
TAATGGA804.489294E-429.6823259
GTGTAAG650.00564942129.4260371
AACGCAG41250.028.5788946
ATCAACG41000.028.5317843
TCCGTAA504.9418355E-528.50552228-29
CAACGCA41250.028.3588665
TATCAAC41350.028.2902832
TCAACGC41400.028.2561174
GATCAGT856.1502086E-428.1278271
TACAATC856.394132E-427.9414467
Produced by FastQC (version 0.11.5)