FastQCFastQC Report
Thu 26 May 2016
SRR938203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271835
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58062.1358544705427924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50081.8422940386631597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43141.58699210918388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29721.093310280133169No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT20530.7552375521915868No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.729118766899038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13520.497360531204591No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT12030.44254786911177735TruSeq Adapter, Index 11 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.3513160556955506No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.3351297662184781No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.2733275700332923No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7090.2608199827100999No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT6510.23948351021759523RNA PCR Primer, Index 11 (95% over 23bp)
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5030.1850387183401696No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5000.18393510769400556No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.17473835230930526No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA4530.16664520757076903No Hit
GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4230.1556091011091287No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.12691522430886384No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3370.12397226258575975No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT3300.12139717107804365No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT3230.11882207957032759TruSeq Adapter, Index 11 (96% over 26bp)
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.11330402633950742No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3060.11256828590873139No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT2970.10925745397023931No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG2960.10888958375485129TruSeq Adapter, Index 11 (96% over 28bp)
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2950.10852171353946329No Hit
GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2920.10741810289329926No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA554.95582E-543.1780559
GGTATCA23050.037.9631541
TAGAGTG702.0436326E-433.9443475
GGGAGCT702.0502732E-433.9256177
TATACAG902.3986016E-531.681395
GTATAGT1751.4915713E-1029.8930321
AGGGGTG1452.3877874E-829.4801888
CTCCTCG650.005838813729.228229
GTATCAA33050.029.2105641
CTATACA1652.6157068E-928.8012684
GTTGCTA700.00838406227.1404939
GTAGGGG1806.657501E-926.386596
ATAGTAG1901.1743396E-825.016233
AGTGTAT1551.4050929E-624.5139928
GGGGTGA1751.478711E-724.4264419
TATCAAC39750.024.2870222
AGTAGGG2351.72804E-1024.2665985
ATCAACG40250.023.9720733
TCAACGC40400.023.8786724
CAACGCA40850.023.7319585
Produced by FastQC (version 0.11.5)