##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938203_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 271835 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.432791950999686 33.0 31.0 34.0 31.0 34.0 2 32.63210771239907 34.0 31.0 34.0 31.0 34.0 3 32.65469126492174 34.0 31.0 34.0 31.0 34.0 4 36.161425129214415 37.0 35.0 37.0 35.0 37.0 5 36.105898798903745 37.0 35.0 37.0 35.0 37.0 6 36.183346515349385 37.0 37.0 37.0 35.0 37.0 7 36.151382272334324 37.0 36.0 37.0 35.0 37.0 8 36.1672411573197 37.0 37.0 37.0 35.0 37.0 9 37.819688413927565 39.0 38.0 39.0 35.0 39.0 10-11 37.82738609818456 39.0 38.0 39.0 35.0 39.0 12-13 37.7692993911748 39.0 38.0 39.0 35.0 39.0 14-15 38.923409421156215 40.0 38.0 41.0 35.5 41.0 16-17 38.85905236632516 40.0 38.0 41.0 35.5 41.0 18-19 39.06074456931594 40.0 38.5 41.0 35.5 41.0 20-21 39.06174149759964 40.0 39.0 41.0 35.0 41.0 22-23 38.914334062942594 40.0 38.0 41.0 35.0 41.0 24-25 38.78646973347803 40.0 38.0 41.0 35.0 41.0 26-27 38.547238214357975 40.0 38.0 41.0 35.0 41.0 28-29 38.36138098478857 40.0 37.5 41.0 34.5 41.0 30-31 38.00348005223758 40.0 36.5 41.0 34.0 41.0 32-33 37.604151415380656 40.0 35.0 41.0 33.5 41.0 34-35 37.3139588353229 40.0 35.0 41.0 33.0 41.0 36-37 37.15434178821712 40.0 35.0 41.0 33.0 41.0 38-39 36.86402781098828 40.0 35.0 41.0 33.0 41.0 40-41 36.60797910497177 39.0 35.0 41.0 32.5 41.0 42-43 36.282785513270916 39.0 35.0 41.0 31.0 41.0 44-45 35.87976897750474 39.0 35.0 41.0 30.5 41.0 46-47 35.324174591204226 39.0 35.0 41.0 27.5 41.0 48-49 34.79573454485258 39.0 35.0 41.0 23.5 41.0 50-51 34.37910681111704 38.5 35.0 40.0 21.0 41.0 52-53 33.91980613239649 38.0 34.5 40.0 17.0 41.0 54-55 33.573531738002835 38.0 34.0 40.0 10.0 41.0 56-57 33.191226295362995 38.0 33.0 40.0 4.0 41.0 58-59 32.69442676623687 37.5 33.0 40.0 2.0 41.0 60-61 32.20134456563724 37.0 32.5 40.0 2.0 41.0 62-63 31.661123475637794 36.0 31.5 39.5 2.0 41.0 64-65 31.125682859087313 36.0 31.0 39.0 2.0 40.0 66-67 30.576941894899477 35.0 30.5 39.0 2.0 40.0 68-69 30.012463442897346 35.0 29.5 38.5 2.0 40.0 70-71 29.490084058344216 35.0 29.0 38.0 2.0 40.0 72-73 28.926560229551015 35.0 28.0 37.0 2.0 39.0 74-75 28.365902109735686 34.0 26.5 36.5 2.0 39.0 76-77 27.04414626519764 32.5 25.5 35.0 2.0 37.5 78-79 27.675968878179777 34.0 26.0 36.0 2.0 38.0 80-81 27.434471278532932 34.0 26.0 35.0 2.0 37.0 82-83 26.974124009049607 34.0 25.5 35.0 2.0 37.0 84-85 26.435701804403408 33.0 24.5 35.0 2.0 36.0 86-87 25.902556697996946 33.0 21.5 35.0 2.0 36.0 88-89 25.269198226865562 32.5 19.0 35.0 2.0 35.0 90-91 24.763238729376276 32.0 12.5 35.0 2.0 35.0 92-93 24.031230341935366 31.5 4.0 34.0 2.0 35.0 94-95 23.348503687898912 31.0 2.0 34.0 2.0 35.0 96-97 22.515963727996763 31.0 2.0 34.0 2.0 35.0 98-99 21.348189158864756 30.0 2.0 34.0 2.0 35.0 100-101 17.67828094248349 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2215504299892146 1101 2 -0.13647627759908687 1101 3 0.04122693544894673 1101 4 -0.18620230998114806 1101 5 -0.11559552328482425 1101 6 -0.11130254489123814 1101 7 0.041050737703315576 1101 8 0.06275949787868029 1101 9 -0.14818190157137678 1101 10-11 -0.09305813970400578 1101 12-13 0.07848139324422476 1101 14-15 0.0485889681575955 1101 16-17 -0.02334191208582581 1101 18-19 0.07219641273552924 1101 20-21 0.054405227755673025 1101 22-23 0.035811548057537834 1101 24-25 0.3376822263687771 1101 26-27 0.2944023956044646 1101 28-29 0.23770589597337022 1101 30-31 0.3555730889975166 1101 32-33 0.4177863373106092 1101 34-35 0.16460494731078512 1101 36-37 0.44880491860367044 1101 38-39 0.26562360298859033 1101 40-41 0.1864482797380873 1101 42-43 0.5376790556849471 1101 44-45 0.38747446988877954 1101 46-47 0.8832239476283306 1101 48-49 0.9913822156579712 1101 50-51 0.5358972735349923 1101 52-53 0.6817956876792906 1101 54-55 0.7800505299400982 1101 56-57 0.6846179141106177 1101 58-59 0.7163301588848441 1101 60-61 0.5270712566487887 1101 62-63 0.3485643353019263 1101 64-65 0.7428437366856713 1101 66-67 0.8707685084088688 1101 68-69 1.1949535876781532 1101 70-71 1.0269279675066194 1101 72-73 1.0734121309544769 1101 74-75 0.6906859632154969 1101 76-77 0.9076352006086239 1101 78-79 0.7159541768451199 1101 80-81 0.8013802331869719 1101 82-83 0.8717295636867064 1101 84-85 0.9826525682353555 1101 86-87 1.0506620501291408 1101 88-89 1.3448970280457928 1101 90-91 1.67574681048335 1101 92-93 1.8483420486654794 1101 94-95 2.0053700030644492 1101 96-97 1.8681725905878857 1101 98-99 2.627436915395446 1101 100-101 2.4492768429058565 1102 1 0.1614413732894704 1102 2 0.30969858578889387 1102 3 0.16561218135058198 1102 4 0.2385927719860632 1102 5 0.15960939474796731 1102 6 0.07749526931640816 1102 7 0.003140901637742388 1102 8 0.14178955252349112 1102 9 0.2296186448767088 1102 10-11 0.24464677832878579 1102 12-13 0.39507975389995664 1102 14-15 0.3152556348242612 1102 16-17 0.2198957928322116 1102 18-19 0.18541362585028054 1102 20-21 0.1588109654605887 1102 22-23 0.41863258630890243 1102 24-25 0.36298960341796516 1102 26-27 0.4983299912328789 1102 28-29 0.5001717429679076 1102 30-31 0.5225471327133704 1102 32-33 0.4437426214636133 1102 34-35 0.5798713407534066 1102 36-37 0.5660180333577642 1102 38-39 0.45414819315252686 1102 40-41 0.6632242360222449 1102 42-43 0.7813607496740076 1102 44-45 0.3658556174297658 1102 46-47 0.632199357464394 1102 48-49 0.6919969697563317 1102 50-51 0.753349459327346 1102 52-53 0.8519459609033291 1102 54-55 0.9612322239291586 1102 56-57 0.8396384059138953 1102 58-59 0.7823479184476909 1102 60-61 0.7257051364302001 1102 62-63 0.6974031331161363 1102 64-65 0.9076661410572555 1102 66-67 0.8955635903760815 1102 68-69 1.1201926587164017 1102 70-71 1.1155208636814855 1102 72-73 1.4073602183861738 1102 74-75 1.7776736681335272 1102 76-77 1.5451010476031612 1102 78-79 1.6421836850418394 1102 80-81 1.9097477195257682 1102 82-83 1.8817705472932644 1102 84-85 2.3192645900932796 1102 86-87 1.9333464763586505 1102 88-89 1.9452385581004386 1102 90-91 2.070828777696466 1102 92-93 1.9971125404687626 1102 94-95 2.420363172463354 1102 96-97 2.433302372008651 1102 98-99 2.551070795176866 1102 100-101 2.952487225419514 1103 1 0.21428001870779667 1103 2 0.25538251673178536 1103 3 0.20859026899998412 1103 4 0.19661070027291316 1103 5 0.047408199528845785 1103 6 0.145489957231419 1103 7 0.16623851119949506 1103 8 0.23131478492720703 1103 9 0.23159407648361707 1103 10-11 0.21835195761165238 1103 12-13 0.30518931565294594 1103 14-15 0.28564076098628277 1103 16-17 0.3873758725134806 1103 18-19 0.22288872545187388 1103 20-21 -0.10286732139200438 1103 22-23 0.038660806760425714 1103 24-25 0.214159922143061 1103 26-27 0.142910004513098 1103 28-29 0.17850009622156193 1103 30-31 0.3685049680387351 1103 32-33 0.09148498534143101 1103 34-35 -0.15924718514300906 1103 36-37 -0.20300587102470757 1103 38-39 -0.5085012092379131 1103 40-41 -0.21791786225133336 1103 42-43 -0.05035405776290247 1103 44-45 -0.5380039443232292 1103 46-47 -0.8665556226152873 1103 48-49 -0.8428137872157748 1103 50-51 -0.7084450293844711 1103 52-53 -0.9196509846477952 1103 54-55 -0.8552933404798608 1103 56-57 -0.6136235462773314 1103 58-59 -0.36176728739560104 1103 60-61 -0.6539097374077762 1103 62-63 -0.7480981949051149 1103 64-65 -0.2794686530994497 1103 66-67 -0.18416748532112948 1103 68-69 -0.6904066108719071 1103 70-71 -0.7373509822680511 1103 72-73 -0.4191504057838138 1103 74-75 -0.024592268121448768 1103 76-77 -0.004160240577451901 1103 78-79 0.05254225077888819 1103 80-81 -0.20714305072655392 1103 82-83 0.23889206123748608 1103 84-85 0.4328436073575581 1103 86-87 0.6299351217769775 1103 88-89 0.2636482526555497 1103 90-91 0.6097730008040365 1103 92-93 0.4704193133771284 1103 94-95 0.2915368112415777 1103 96-97 0.7756247491666812 1103 98-99 1.3199618974344993 1103 100-101 1.595797318380999 1104 1 0.40831637328947323 1104 2 0.3313131691222253 1104 3 0.2538934313505834 1104 4 0.2049990219860618 1104 5 0.2080468947479659 1104 6 0.17801610264974244 1104 7 0.1828284016377424 1104 8 0.25064371919015827 1104 9 0.20722281154337452 1104 10-11 0.42199052832878436 1104 12-13 0.31304850389995664 1104 14-15 0.7927295931575955 1104 16-17 0.6284895428322059 1104 18-19 0.5526011258502805 1104 20-21 0.5049047154605901 1104 22-23 0.5880336279755696 1104 24-25 0.6708021034179623 1104 26-27 0.6153872828995475 1104 28-29 0.6186613263012433 1104 30-31 0.6437710910467018 1104 32-33 0.5440030381302776 1104 34-35 0.6392463407534095 1104 36-37 0.5371117833577657 1104 38-39 0.5557106931525269 1104 40-41 0.5310627776889092 1104 42-43 0.307402416340679 1104 44-45 0.5049181174297601 1104 46-47 0.848996232464394 1104 48-49 1.1888719697563346 1104 50-51 1.0747036259940117 1104 52-53 0.8790292942366662 1104 54-55 1.42086764059583 1104 56-57 1.3521384059138981 1104 58-59 1.4271395851143538 1104 60-61 1.67739784476354 1104 62-63 1.5549552164494678 1104 64-65 1.7304525993905884 1104 66-67 1.8693917153760822 1104 68-69 1.8446718253830703 1104 70-71 2.141041697014817 1104 72-73 2.09512063505284 1104 74-75 1.9636111681335287 1104 76-77 1.6831218809364934 1104 78-79 1.6011680600418394 1104 80-81 1.5669091778591024 1104 82-83 1.6579424222932637 1104 84-85 1.9226500067599481 1104 86-87 1.9645964763586505 1104 88-89 2.302920849767105 1104 90-91 1.9044225276964646 1104 92-93 2.46703441546876 1104 94-95 2.0686704641300224 1104 96-97 2.251401330341981 1104 98-99 2.9887010035101973 1104 100-101 2.489075767086181 1105 1 0.17871236565588333 1105 2 0.2056273389695562 1105 3 0.2670434790605043 1105 4 0.2306729246578172 1105 5 0.1889032878777357 1105 6 0.11235532020699424 1105 7 0.3461752527827784 1105 8 0.40110889094588487 1105 9 0.2601211894059716 1105 10-11 0.18853990809977716 1105 12-13 0.11215990657171204 1105 14-15 0.3615660673942358 1105 16-17 0.12476220504594693 1105 18-19 0.20187354951440284 1105 20-21 0.09445027843768372 1105 22-23 0.05319047434961277 1105 24-25 0.09500296219658821 1105 26-27 -0.08342891461953172 1105 28-29 0.21971690836230806 1105 30-31 -0.17145732021283777 1105 32-33 0.24018028049669482 1105 34-35 0.15797248579158207 1105 36-37 0.16124704099135556 1105 38-39 -0.08115715035892634 1105 40-41 -0.08423123471567351 1105 42-43 -0.144291285949393 1105 44-45 0.15502546475800472 1105 46-47 0.09069172387660984 1105 48-49 -0.029281656197866823 1105 50-51 0.028842461871875003 1105 52-53 0.4899548667557454 1105 54-55 0.35285118067216104 1105 56-57 0.5055258104940492 1105 58-59 0.2695775622135912 1105 60-61 0.34567969113758323 1105 62-63 0.39724410003725197 1105 64-65 0.5834613064516567 1105 66-67 0.7580635903760822 1105 68-69 1.299884134543376 1105 70-71 1.3916977084651982 1105 72-73 1.440152839251315 1105 74-75 1.925622141415971 1105 76-77 1.849569877119702 1105 78-79 1.3234813949655013 1105 80-81 1.7907432665995593 1105 82-83 1.6099194022550947 1105 84-85 1.5426106460729265 1105 86-87 1.5695583084197189 1105 88-89 1.9274586598816086 1105 90-91 2.265676105940738 1105 92-93 2.2207766626061627 1105 94-95 2.955970678824677 1105 96-97 3.547217766410686 1105 98-99 3.175052983472032 1105 100-101 3.7938676325441953 1106 1 -0.0137962692960798 1106 2 -0.018184810915798266 1106 3 -0.11675473880151088 1106 4 -0.10166388200633492 1106 5 -0.21534307673492492 1106 6 -0.25761246198904075 1106 7 -0.058883813191151546 1106 8 0.10232504048293123 1106 9 -0.03674700784826257 1106 10-11 0.04768860342384329 1106 12-13 -0.03576625370460107 1106 14-15 -0.24989011929487503 1106 16-17 0.02022849245197733 1106 18-19 -0.12055120304705724 1106 20-21 -0.16478694328466048 1106 22-23 -0.2984697584312741 1106 24-25 -0.22781362852119713 1106 26-27 -0.4350746348761163 1106 28-29 -0.1666137449915297 1106 30-31 0.11612286148396578 1106 32-33 0.3128807710200121 1106 34-35 0.10460993390930184 1106 36-37 -0.06069299325820765 1106 38-39 0.01641055056698093 1106 40-41 -0.18531822278637122 1106 42-43 -0.16782334411559674 1106 44-45 -0.5734742304789862 1106 46-47 -0.28201166915157927 1106 48-49 -0.24749922796229384 1106 50-51 -0.2792947105078909 1106 52-53 -0.4796921886530683 1106 54-55 -0.3584366607349665 1106 56-57 -0.02060398952336584 1106 58-59 -0.006617544163212585 1106 60-61 -0.2863987925938787 1106 62-63 -0.5846152445771473 1106 64-65 -0.252489118765304 1106 66-67 0.22194191737228053 1106 68-69 0.028042785459117425 1106 70-71 0.08730476735702197 1106 72-73 0.2632379116688064 1106 74-75 0.37066321185976747 1106 76-77 0.4101596661075959 1106 78-79 0.7923737991102797 1106 80-81 0.20950215552070262 1106 82-83 0.4018751100309039 1106 84-85 0.36910616170291277 1106 86-87 0.5349386816818438 1106 88-89 0.5934166316112126 1106 90-91 0.27643714271547637 1106 92-93 0.35414676100108267 1106 94-95 0.6290688125140527 1106 96-97 1.0673248688400783 1106 98-99 1.5240429117801604 1106 100-101 1.5613116867629877 1107 1 0.16372736550736988 1107 2 0.1796077946086072 1107 3 0.22694486617548648 1107 4 0.31247409494326206 1107 5 -0.12010850408471896 1107 6 -0.014074121085663194 1107 7 0.017975531987936222 1107 8 0.2372986316415222 1107 9 0.15654795745776795 1107 10-11 0.09795417132489348 1107 12-13 0.0869571858065683 1107 14-15 0.28594305376071105 1107 16-17 0.30943859438863086 1107 18-19 0.19015584374911754 1107 20-21 0.08821271643335393 1107 22-23 0.11713291056312158 1107 24-25 0.1183641170366414 1107 26-27 0.17304626879448648 1107 28-29 0.178957410931595 1107 30-31 0.19948455164981738 1107 32-33 -0.026750244943649193 1107 34-35 0.04385480378842743 1107 36-37 -0.1953632895994346 1107 38-39 -0.5336922737735463 1107 40-41 -0.732365906649612 1107 42-43 -0.6094871750990123 1107 44-45 -0.28864340980759806 1107 46-47 -0.031467957710702876 1107 48-49 0.08533354563181916 1107 50-51 0.012112429495957144 1107 52-53 0.19651470279698202 1107 54-55 0.20049130304718688 1107 56-57 0.1236607794547595 1107 58-59 -0.011758761189149425 1107 60-61 0.11292952034720116 1107 62-63 0.12932596061289203 1107 64-65 0.21877184793144266 1107 66-67 0.18218810399476126 1107 68-69 0.26669881954649455 1107 70-71 0.4764320180264896 1107 72-73 0.6352622936131525 1107 74-75 0.4052758082113499 1107 76-77 0.4578361319092572 1107 78-79 0.2666973037188818 1107 80-81 0.31732716180852094 1107 82-83 0.20944564456953074 1107 84-85 -0.038485085652503415 1107 86-87 -0.06458640301877949 1107 88-89 0.574405224767105 1107 90-91 0.8059455092139736 1107 92-93 1.149252618289772 1107 94-95 1.066382951970489 1107 96-97 1.208926561373108 1107 98-99 1.5664080195607788 1107 100-101 2.2892414407243145 1108 1 0.10315824075935609 1108 2 0.32696766209411265 1108 3 0.1875499323545995 1108 4 0.15541004106236755 1108 5 0.16528208551101642 1108 6 0.1722743857822735 1108 7 0.23331158436866417 1108 8 0.23862690192108005 1108 9 0.24402627539879518 1108 10-11 -0.036076113237477614 1108 12-13 0.2417163000846969 1108 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76-77 0.6345342307863433 2316 78-79 0.6151566580148113 2316 80-81 0.6765833051113539 2316 82-83 0.5654417184644345 2316 84-85 0.6992195450482335 2316 86-87 0.7243562361184104 2316 88-89 1.0203511707130488 2316 90-91 1.286519468387155 2316 92-93 1.2355509789071988 2316 94-95 1.5948939532441386 2316 96-97 1.4690193389756168 2316 98-99 1.9353801044861747 2316 100-101 2.3929151533474418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 5.0 5 15.0 6 42.0 7 91.0 8 169.0 9 269.0 10 535.0 11 874.0 12 1241.0 13 1936.0 14 2270.0 15 3133.0 16 3804.0 17 5141.0 18 4976.0 19 3484.0 20 2906.0 21 3125.0 22 3326.0 23 3537.0 24 3806.0 25 4010.0 26 3928.0 27 3961.0 28 4566.0 29 5333.0 30 6477.0 31 7929.0 32 9749.0 33 12823.0 34 19128.0 35 29927.0 36 33143.0 37 49949.0 38 34419.0 39 1808.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.78025272683797 23.746390273511505 15.922526532639287 19.550830467011238 2 14.56717538153615 24.940396473825956 40.30890815170201 20.183519992935878 3 17.080474570035868 30.990159109721326 32.22330543548239 19.706060884760415 4 10.923819474079197 22.25892844004297 38.34409994555381 28.473152140324025 5 9.654834599038015 41.743578789195716 35.46894910909613 13.132637502670134 6 24.252947560100797 41.63518310739971 18.317729505030623 15.79413982746887 7 22.28815273971343 38.59583938786396 22.806849743410528 16.309158129012086 8 23.359390806923315 36.34815237184321 21.738186767708353 18.554270053525116 9 24.6701123843508 18.724593963249767 22.607096216454835 33.9981974359446 10-11 21.812864421432117 30.86872551363879 27.550352235731236 19.76805782919786 12-13 23.73149152978829 29.965788069968912 28.3399856530616 17.962734747181194 14-15 20.82384534736145 31.732300844262145 27.75176117865617 19.692092629720236 16-17 16.96323137197197 34.85441536226019 29.221954494454355 18.96039877131348 18-19 16.355141905935586 31.9454816340795 34.062390788529804 17.63698567145511 20-21 17.522026229146356 31.032979564809537 34.46704802545662 16.977946180587487 22-23 17.522761969577132 31.424761344197766 34.29598837530119 16.756488310923906 24-25 17.240180552030107 32.34430698259593 34.2730647125257 16.14244775284826 26-27 16.412908115307005 32.75094872035421 34.771819648398626 16.064323515940156 28-29 15.903213346331412 32.185884819835564 35.31977118472603 16.591130649106994 30-31 16.662865341107658 31.82997038644766 35.25944046940239 16.247723803042284 32-33 15.611308330421029 33.170673386429264 34.88733974653742 16.330678536612282 34-35 16.218513210976433 32.64489947590236 35.051260023436306 16.085327289684898 36-37 16.7800937336757 31.901270628398215 34.43112340619643 16.88751223172966 38-39 15.926449163392345 32.19559842832546 34.866378232013304 17.011574176268894 40-41 15.901494289030783 31.864614433695177 35.621952049565586 16.611939227708454 42-43 16.330194562120262 31.218602324002866 35.0761821897574 17.375020924119486 44-45 15.891503525252507 31.526223721555635 34.80168270336191 17.780590049829943 46-47 16.930490922802434 30.954071403608808 34.051354682068165 18.064082991520593 48-49 16.514429709198595 31.03500285099417 33.958099582467305 18.492467857339932 50-51 16.08383762208693 30.917652252285393 34.2222303971159 18.77627972851178 52-53 16.374271157135762 31.267680762227084 34.50935310022624 17.84869498041091 54-55 16.666360108153842 30.860816304007948 33.629223609910426 18.843599977927784 56-57 17.013997461695514 31.361487667151028 32.70531756396343 18.91919730719002 58-59 16.15042213107216 31.286993948534956 33.743815182003786 18.818768738389096 60-61 16.546434417937352 32.41433222359152 32.371475343498815 18.667758014972318 62-63 16.00033108319385 32.52138245626943 32.5316828223003 18.946603638236432 64-65 16.24386116578071 32.05933746574209 32.304522964298194 19.392278404179006 66-67 16.52160317839866 32.25706770651314 31.5005426085677 19.7207865065205 68-69 16.210961569943144 31.482186575139377 32.195496106963326 20.111355747954153 70-71 16.686225099784796 31.023782809424834 32.07276472860375 20.21722736218662 72-73 16.560781356337483 30.364743318557213 32.06136075192672 21.013114573178584 74-75 16.361027829381793 30.96988982287049 31.296374639027352 21.372707708720363 76-77 16.333989368550775 30.29834274467968 32.285209777990325 21.08245810877922 78-79 16.205050858057277 29.7790939356595 32.10660878841944 21.909246417863777 80-81 16.550541874820663 30.19563410022293 31.387537982739467 21.86628604221694 82-83 16.68058478155304 29.66269111349824 31.562605765410478 22.094118339538237 84-85 16.236335443142753 29.685220849405226 31.50789752984911 22.57054617760291 86-87 16.37481652068028 29.929477723127405 31.47953308881687 22.216172667375446 88-89 16.5694263063991 29.616311365350306 31.1076572185333 22.706605109717295 90-91 16.554186417389097 29.0061086984113 31.546525252413772 22.893179631785834 92-93 16.669670940092335 29.160704103592256 31.205142825611127 22.964482130704287 94-95 16.32957492596612 28.781245976419516 31.643276252138246 23.245902845476117 96-97 16.522185373821205 28.834640194677274 31.392262571527894 23.250911859973623 98-99 16.4958522633215 28.921404528482352 31.00428568800927 23.578457520186877 100-101 16.447845200213365 28.230544263983663 31.746463847554583 23.575146688248385 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5204.0 1 2996.0 2 908.5 3 1008.0 4 760.5 5 687.5 6 1365.0 7 2446.5 8 3157.5 9 3092.0 10 2243.0 11 1426.5 12 1208.5 13 1137.5 14 992.0 15 866.5 16 815.0 17 802.0 18 846.5 19 917.5 20 999.0 21 1052.0 22 1136.5 23 1285.5 24 1455.0 25 1750.5 26 2232.0 27 2806.5 28 3077.0 29 3429.5 30 4193.0 31 5020.5 32 6062.0 33 7607.5 34 9169.5 35 10088.5 36 11157.0 37 12505.5 38 12713.5 39 12135.0 40 11449.5 41 10523.0 42 9680.5 43 10039.5 44 10348.0 45 9729.5 46 8922.5 47 8495.5 48 8958.0 49 8415.0 50 7070.0 51 5833.5 52 4501.0 53 3486.5 54 2822.5 55 2359.5 56 2015.5 57 1611.5 58 1253.5 59 1022.0 60 794.0 61 614.5 62 479.5 63 352.5 64 264.0 65 181.0 66 123.0 67 76.5 68 49.5 69 41.5 70 33.0 71 30.5 72 25.0 73 17.0 74 15.5 75 13.5 76 8.0 77 5.5 78 4.0 79 2.5 80 4.5 81 3.5 82 2.0 83 1.0 84 1.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014346938400132432 3 0.0036787021538801108 4 0.002575091507716078 5 0.11514337741644748 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.357404307760222E-4 26-27 0.007909209630842238 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012691522430886384 36-37 3.678702153880111E-4 38-39 0.008461014953924255 40-41 0.012323652215498374 42-43 0.007173469200066216 44-45 0.0038626372615741165 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.001287545753858039 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.678702153880111E-4 82-83 0.002575091507716078 84-85 0.0034947670461861055 86-87 0.002207221292328067 88-89 0.0 90-91 0.0034947670461861055 92-93 0.0 94-95 0.0 96-97 1.8393510769400555E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 271835.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.14227835311798 #Duplication Level Percentage of deduplicated Percentage of total 1 74.6296090288158 29.21172929990275 2 12.671694222837186 9.919979649517803 3 4.351562715660908 5.109902372623478 4 2.1832485183811157 3.4182928488202418 5 1.2283332929898563 2.4039881832305485 6 0.7570325356134365 1.7779186938808704 7 0.5428608806112839 1.4874168187133936 8 0.44524397084153783 1.3942290753381616 9 0.304797703954188 1.0737428912609466 >10 2.5190630738827746 19.794841577183593 >50 0.2619666218378431 7.0397600800683895 >100 0.09064244329750465 5.998377759850032 >500 0.005976424832802505 1.7435648581117544 >1k 0.005976424832802505 5.410041585679496 >5k 0.001992141610934168 4.216214305818541 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5806 2.1358544705427924 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5008 1.8422940386631597 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4314 1.58699210918388 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2972 1.093310280133169 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2053 0.7552375521915868 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1982 0.729118766899038 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1352 0.497360531204591 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1203 0.44254786911177735 TruSeq Adapter, Index 11 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 955 0.3513160556955506 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 911 0.3351297662184781 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 743 0.2733275700332923 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 709 0.2608199827100999 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 651 0.23948351021759523 RNA PCR Primer, Index 11 (95% over 23bp) GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 503 0.1850387183401696 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 500 0.18393510769400556 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 475 0.17473835230930526 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 453 0.16664520757076903 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 423 0.1556091011091287 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 345 0.12691522430886384 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 337 0.12397226258575975 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 330 0.12139717107804365 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 323 0.11882207957032759 TruSeq Adapter, Index 11 (96% over 26bp) GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 308 0.11330402633950742 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 306 0.11256828590873139 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 297 0.10925745397023931 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 296 0.10888958375485129 TruSeq Adapter, Index 11 (96% over 28bp) GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 295 0.10852171353946329 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 292 0.10741810289329926 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.518053230820167E-4 0.0 20-21 0.0 0.0 0.0 0.0014714808615520444 0.0 22-23 0.0 0.0 0.0 0.005701988338514172 0.0 24-25 0.0 0.0 0.0 0.014898743723214451 0.0 26-27 0.0 0.0 0.0 0.03697095664649512 0.0 28-29 0.0 0.0 0.0 0.08001177184689241 0.0 30-31 0.0 0.0 0.0 0.17400261187852925 0.0 32-33 0.0 0.0 0.0 0.3255651406183898 0.0 34-35 0.0 0.0 0.0 0.5591627273897769 0.0 36-37 0.0 0.0 0.0 0.897419390439053 0.0 38-39 0.0 0.0 0.0 1.2827634410579947 0.0 40-41 0.0 0.0 0.0 1.6320562105689111 0.0 42-43 0.0 0.0 0.0 1.9767506023874777 0.0 44-45 0.0 0.0 0.0 2.370923538175732 0.0 46-47 0.0 0.0 0.0 2.818437655195247 0.0 48-49 0.0 0.0 0.0 3.2356024794452516 0.0 50-51 0.0 0.0 0.0 3.6266485184027077 0.0 52-53 0.0 0.0 0.0 3.9963580848676585 0.0 54-55 0.0 0.0 0.0 4.394761528132875 0.0 56-57 0.0 0.0 0.0 4.88862729229128 0.0 58-59 0.0 0.0 0.0 5.3462578402339656 0.0 60-61 0.0 0.0 0.0 5.830742913899976 0.0 62-63 0.0 0.0 0.0 6.2663012489193815 0.0 64-65 0.0 0.0 0.0 6.730921330954439 0.0 66-67 0.0 0.0 0.0 7.276656795482554 0.0 68-69 0.0 0.0 0.0 7.835083782441554 0.0 70-71 0.0 0.0 0.0 8.429378115400887 0.0 72-73 0.0 0.0 0.0 8.980999503375209 0.0 74-75 0.0 0.0 0.0 9.557636065995917 0.0 76-77 0.0 0.0 0.0 10.159287803263009 0.0 78-79 0.0 0.0 0.0 10.816120072838302 0.0 80-81 0.0 0.0 0.0 11.486747475490647 0.0 82-83 0.0 0.0 0.0 12.129600676881196 0.0 84-85 0.0 0.0 0.0 12.753876432394652 0.0 86-87 0.0 0.0 0.0 13.403719167877572 0.0 88-89 0.0 0.0 0.0 14.09145253554546 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 55 4.95582E-5 43.178055 9 GGTATCA 2305 0.0 37.963154 1 TAGAGTG 70 2.0436326E-4 33.944347 5 GGGAGCT 70 2.0502732E-4 33.925617 7 TATACAG 90 2.3986016E-5 31.68139 5 GTATAGT 175 1.4915713E-10 29.893032 1 AGGGGTG 145 2.3877874E-8 29.480188 8 CTCCTCG 65 0.0058388137 29.22822 9 GTATCAA 3305 0.0 29.210564 1 CTATACA 165 2.6157068E-9 28.801268 4 GTTGCTA 70 0.008384062 27.140493 9 GTAGGGG 180 6.657501E-9 26.38659 6 ATAGTAG 190 1.1743396E-8 25.01623 3 AGTGTAT 155 1.4050929E-6 24.513992 8 GGGGTGA 175 1.478711E-7 24.426441 9 TATCAAC 3975 0.0 24.287022 2 AGTAGGG 235 1.72804E-10 24.266598 5 ATCAACG 4025 0.0 23.972073 3 TCAACGC 4040 0.0 23.878672 4 CAACGCA 4085 0.0 23.731958 5 >>END_MODULE