##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938202_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 268006 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.698357499458968 33.0 31.0 34.0 30.0 34.0 2 31.842443825884494 34.0 31.0 34.0 30.0 34.0 3 31.879196734401468 34.0 31.0 34.0 30.0 34.0 4 35.3653537607367 37.0 35.0 37.0 35.0 37.0 5 35.386789847988474 37.0 35.0 37.0 35.0 37.0 6 35.45517264538854 37.0 36.0 37.0 35.0 37.0 7 35.41682275769946 37.0 36.0 37.0 35.0 37.0 8 35.4136959620307 37.0 36.0 37.0 35.0 37.0 9 37.046151951821976 39.0 38.0 39.0 35.0 39.0 10-11 36.99458594210577 39.0 37.5 39.0 34.5 39.0 12-13 36.92110437826018 39.0 37.0 39.0 34.0 39.0 14-15 38.09390088281606 40.0 38.0 41.0 34.0 41.0 16-17 37.97953404028268 40.0 38.0 41.0 33.5 41.0 18-19 38.08445146750446 40.0 38.0 41.0 34.0 41.0 20-21 38.079684783176496 40.0 38.0 41.0 34.0 41.0 22-23 37.954008492347185 40.0 38.0 41.0 34.0 41.0 24-25 37.84104460347903 40.0 38.0 41.0 34.0 41.0 26-27 37.6136989470385 40.0 37.5 41.0 33.5 41.0 28-29 37.37459609113229 40.0 37.0 41.0 33.0 41.0 30-31 37.054683477235585 40.0 36.0 41.0 33.0 41.0 32-33 36.6312862398603 39.0 35.0 41.0 32.5 41.0 34-35 36.354646910890054 39.0 35.0 41.0 31.5 41.0 36-37 36.06003223808422 39.0 35.0 41.0 31.0 41.0 38-39 35.73465146302695 39.0 35.0 41.0 30.0 41.0 40-41 35.42794377737812 39.0 35.0 41.0 29.5 41.0 42-43 35.08223696484407 39.0 35.0 41.0 26.0 41.0 44-45 34.67728334440274 39.0 35.0 41.0 23.5 41.0 46-47 34.144420274172965 38.0 35.0 41.0 16.0 41.0 48-49 33.65927255359955 38.0 35.0 40.0 4.0 41.0 50-51 32.9024555420401 37.5 33.5 39.5 2.0 40.5 52-53 32.95296560524764 38.0 33.5 40.0 2.0 40.5 54-55 33.031165347044464 38.0 34.0 40.0 2.0 41.0 56-57 32.74882838443915 38.0 34.0 40.0 2.0 41.0 58-59 32.49372775236375 38.0 33.5 40.0 2.0 41.0 60-61 32.15208241606531 38.0 33.0 40.0 2.0 41.0 62-63 31.693919165988824 37.0 32.5 40.0 2.0 41.0 64-65 31.25180406408812 36.5 32.0 40.0 2.0 41.0 66-67 30.80372454348037 36.0 31.0 39.0 2.0 41.0 68-69 30.284372364797804 35.0 30.5 39.0 2.0 40.0 70-71 29.88144108713984 35.0 30.0 38.5 2.0 40.0 72-73 29.346600449243674 35.0 29.0 37.5 2.0 40.0 74-75 28.77765050036193 35.0 28.5 37.0 2.0 39.0 76-77 28.20498048551152 34.5 26.5 36.5 2.0 39.0 78-79 27.577272523749468 34.0 26.0 36.0 2.0 38.5 80-81 26.995378834802207 34.0 25.0 35.5 2.0 37.0 82-83 26.378806817757813 33.5 22.5 35.0 2.0 37.0 84-85 25.824052446587018 33.0 20.0 35.0 2.0 36.0 86-87 25.271417430953036 33.0 16.0 35.0 2.0 36.0 88-89 24.579070990947965 32.0 4.5 35.0 2.0 35.0 90-91 23.889062185174957 31.5 2.0 35.0 2.0 35.0 92-93 23.15536219338373 31.0 2.0 34.0 2.0 35.0 94-95 22.382064207517743 31.0 2.0 34.0 2.0 35.0 96-97 21.41954098042581 30.0 2.0 34.0 2.0 35.0 98-99 20.227091930777668 29.0 2.0 34.0 2.0 35.0 100-101 16.668031685857777 20.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18274505282878195 1101 2 0.3831744792531815 1101 3 0.3975047567341754 1101 4 0.27767944065844574 1101 5 0.3210675306337123 1101 6 0.2004300768149534 1101 7 0.31519312199667127 1101 8 0.4484790839659496 1101 9 0.37324213329542033 1101 10-11 0.3196386677325549 1101 12-13 0.4377424501917204 1101 14-15 0.17840602407297723 1101 16-17 0.6303991846131893 1101 18-19 0.4978336763955795 1101 20-21 0.5976104483383153 1101 22-23 0.5719349515536933 1101 24-25 0.3434400460195164 1101 26-27 0.8120329221514595 1101 28-29 0.8466400642698702 1101 30-31 0.8900161999441067 1101 32-33 0.6443067360248946 1101 34-35 0.9154018106010966 1101 36-37 0.880952135171249 1101 38-39 1.1398713799064524 1101 40-41 1.2054088005738848 1101 42-43 1.223287975285885 1101 44-45 1.2238425879374333 1101 46-47 1.1761744275076254 1101 48-49 1.3238203741843009 1101 50-51 1.300589931713695 1101 52-53 0.9159509898264915 1101 54-55 1.0586534949254656 1101 56-57 1.1765331827551364 1101 58-59 0.7887511979303312 1101 60-61 0.5662035904572349 1101 62-63 0.8225647410909254 1101 64-65 1.189281619419301 1101 66-67 0.9585195758785012 1101 68-69 1.3959834316047868 1101 70-71 1.413901018692851 1101 72-73 1.3055626921843135 1101 74-75 1.5853280253074225 1101 76-77 1.623013048508838 1101 78-79 1.5605920792811396 1101 80-81 1.8274405111652499 1101 82-83 2.1437752964255417 1101 84-85 2.16272255363571 1101 86-87 2.1567619111455762 1101 88-89 2.5079139170318108 1101 90-91 2.3334427493309136 1101 92-93 2.5927286775722607 1101 94-95 3.080606527575174 1101 96-97 3.3894578814234 1101 98-99 3.1063042136328427 1101 100-101 3.17139209554043 1102 1 0.6364071363824557 1102 2 0.7645864306016783 1102 3 0.7754793733391487 1102 4 0.5756699218853072 1102 5 0.5259194608293782 1102 6 0.7448510181158525 1102 7 0.6841777861955052 1102 8 0.7335795599046051 1102 9 0.6143182834805074 1102 10-11 0.8048977898901413 1102 12-13 0.8622863423122951 1102 14-15 0.95600385590798 1102 16-17 0.9565824209960638 1102 18-19 0.9855385944283697 1102 20-21 0.8187897185657036 1102 22-23 0.6856644597504129 1102 24-25 0.5213882215879906 1102 26-27 0.4858166344729824 1102 28-29 0.4208732742116936 1102 30-31 0.3280450206738763 1102 32-33 0.27832973972664377 1102 34-35 0.3484398856936437 1102 36-37 0.4541607549491431 1102 38-39 0.4435136326827589 1102 40-41 0.4331322273322158 1102 42-43 0.44750796470947307 1102 44-45 0.30029023468518545 1102 46-47 0.6792481979994278 1102 48-49 0.7779782271721345 1102 50-51 0.3616356747068252 1102 52-53 0.5785964155271799 1102 54-55 0.6402571437884959 1102 56-57 0.7196981748228239 1102 58-59 0.756030415275653 1102 60-61 0.7925388627999084 1102 62-63 0.8167477130634317 1102 64-65 0.6679635337503385 1102 66-67 0.4508516752967999 1102 68-69 0.3645847007745395 1102 70-71 0.5359925046791041 1102 72-73 0.9643741675941477 1102 74-75 0.7821815419652758 1102 76-77 0.7880381674935002 1102 78-79 0.4153316350717269 1102 80-81 0.34568482634240283 1102 82-83 0.936477570354679 1102 84-85 1.159086911118525 1102 86-87 1.203099827591899 1102 88-89 1.9374155034940017 1102 90-91 1.7120453405524927 1102 92-93 2.1774721995183235 1102 94-95 2.790548357294899 1102 96-97 3.23249727856777 1102 98-99 3.572327481744953 1102 100-101 3.628788975498125 1103 1 0.3779146570855154 1103 2 0.37993204493325905 1103 3 0.43641396857321624 1103 4 0.21366214767540725 1103 5 0.34314102581163297 1103 6 0.16601038941887936 1103 7 0.36125231720784257 1103 8 0.4800304640790216 1103 9 0.7371846008710818 1103 10-11 0.5487291233361447 1103 12-13 0.5689155463007936 1103 14-15 0.6018970450240886 1103 16-17 0.7641496003098993 1103 18-19 0.61767903722032 1103 20-21 0.8866027995188901 1103 22-23 1.022321549491842 1103 24-25 0.7708178311874505 1103 26-27 0.5836491509509258 1103 28-29 0.8828472475089768 1103 30-31 1.0723199910980838 1103 32-33 1.1222540256823663 1103 34-35 0.8828111887188257 1103 36-37 1.428112926657775 1103 38-39 1.054549630654705 1103 40-41 0.8761354384189133 1103 42-43 0.7920026410657357 1103 44-45 1.0811546930255602 1103 46-47 1.2631589635900937 1103 48-49 1.0660676305860264 1103 50-51 1.071644969957795 1103 52-53 1.5976728055897098 1103 54-55 1.1774803988163782 1103 56-57 1.095015058378685 1103 58-59 0.9805952285256083 1103 60-61 1.227535482708653 1103 62-63 1.338485924995151 1103 64-65 1.29123123697833 1103 66-67 1.1118285216716508 1103 68-69 0.9053781771984006 1103 70-71 0.818124497242902 1103 72-73 1.2984750633183317 1103 74-75 1.1352531999000384 1103 76-77 0.8064807904443221 1103 78-79 0.8029224750244062 1103 80-81 1.193918728657767 1103 82-83 1.1961530815934829 1103 84-85 1.4669709739882215 1103 86-87 1.4345304512187376 1103 88-89 1.7812552871681575 1103 90-91 2.051829014711693 1103 92-93 2.396311983192483 1103 94-95 2.3360105847750425 1103 96-97 2.3661165112870535 1103 98-99 2.9623900134333034 1103 100-101 3.460904067605611 1104 1 0.18924725494757766 1104 2 0.19084618500249562 1104 3 0.4194664279637941 1104 4 0.30505713379021415 1104 5 0.2208562462628052 1104 6 -0.02954254549191404 1104 7 0.1991950265318465 1104 8 -0.19172743906702294 1104 9 0.30136080964347656 1104 10-11 0.3415259430569222 1104 12-13 0.5165797909844798 1104 14-15 0.38285341128878514 1104 16-17 0.5828527615683257 1104 18-19 0.5351953000118144 1104 20-21 0.5346417541352437 1104 22-23 0.43563421354631515 1104 24-25 0.6136013363420929 1104 26-27 0.45061761444998893 1104 28-29 0.4835434091097639 1104 30-31 0.45900352288185076 1104 32-33 0.5874459456428056 1104 34-35 0.2708356875205098 1104 36-37 -0.042338246926121315 1104 38-39 -0.2517709840978384 1104 40-41 -0.41911506563251777 1104 42-43 -0.19905757881481634 1104 44-45 -0.08614283046513549 1104 46-47 -0.1713809230458807 1104 48-49 0.3628112681254976 1104 50-51 0.14691031024006662 1104 52-53 0.1569416457007904 1104 54-55 0.26579401391128954 1104 56-57 0.4281550134895724 1104 58-59 0.4240178328547515 1104 60-61 0.48991802921452887 1104 62-63 0.4278647053808946 1104 64-65 0.4887623360006579 1104 66-67 0.29065265527381356 1104 68-69 0.28409199010972586 1104 70-71 0.5931653919817457 1104 72-73 0.42249073646475566 1104 74-75 0.37938528039610375 1104 76-77 0.3931966576029957 1104 78-79 0.49343489561252696 1104 80-81 0.5069727097130432 1104 82-83 0.4965011623938764 1104 84-85 0.2673380755973831 1104 86-87 0.21068406327032108 1104 88-89 0.3835016446832782 1104 90-91 0.5380389283572242 1104 92-93 0.5105509606338039 1104 94-95 0.6385460585833904 1104 96-97 0.43822439731436447 1104 98-99 1.0497335672812156 1104 100-101 1.0783004993018857 1105 1 0.3991429022911497 1105 2 0.2819199989664405 1105 3 0.23800654884886896 1105 4 0.3386113403000195 1105 5 0.32778796074124017 1105 6 0.3724730875676414 1105 7 0.2488848782690738 1105 8 0.32795937070429915 1105 9 0.36920987523090787 1105 10-11 0.1587963738257443 1105 12-13 0.44558295198383036 1105 14-15 0.48463362263927934 1105 16-17 0.470676962390975 1105 18-19 0.6452351100873344 1105 20-21 0.5814814160802513 1105 22-23 0.31588656445692465 1105 24-25 0.5544615513958533 1105 26-27 0.35773184688263626 1105 28-29 0.36411318254943836 1105 30-31 0.5654194257505551 1105 32-33 0.5464124707919211 1105 34-35 1.042641953970275 1105 36-37 0.9134342140242921 1105 38-39 0.9398265770390637 1105 40-41 0.9710898041581189 1105 42-43 1.2291123480457458 1105 44-45 0.7908228746757828 1105 46-47 0.3152873307334332 1105 48-49 0.5171447469441617 1105 50-51 0.877874877950255 1105 52-53 1.0071248249519371 1105 54-55 0.9253649644595043 1105 56-57 0.7038629318590779 1105 58-59 0.8465468968550667 1105 60-61 0.8023147015683492 1105 62-63 0.40096975901207443 1105 64-65 0.5837708667311254 1105 66-67 0.9522471744448104 1105 68-69 1.374478055260699 1105 70-71 1.0518938502340696 1105 72-73 0.8815035523993693 1105 74-75 0.9585359106120812 1105 76-77 0.7258356291539982 1105 78-79 0.6562462011449348 1105 80-81 1.3803078946777951 1105 82-83 1.288040529400451 1105 84-85 1.485751227470839 1105 86-87 1.5707404057692322 1105 88-89 1.8688010138060065 1105 90-91 2.0258710647431 1105 92-93 1.9417430144898233 1105 94-95 2.249961366284854 1105 96-97 2.164771501495082 1105 98-99 2.766698478865816 1105 100-101 3.7827272209884555 1106 1 0.19753977423640734 1106 2 0.16431817426784434 1106 3 -0.14167412889632303 1106 4 0.08389644945609831 1106 5 0.26237251597095934 1106 6 -0.06924734253988873 1106 7 -0.07196230322327324 1106 8 0.139168233526064 1106 9 0.1539606083687346 1106 10-11 0.1961049433923776 1106 12-13 0.4369286672005117 1106 14-15 0.42448373668294437 1106 16-17 0.2486763107129022 1106 18-19 0.28010640366830586 1106 20-21 0.1993113281037111 1106 22-23 0.2676167697355112 1106 24-25 0.16087406361481982 1106 26-27 0.0053173796294672115 1106 28-29 0.0684142578182545 1106 30-31 -0.21428731911747434 1106 32-33 0.04208532840026891 1106 34-35 0.08689741177411747 1106 36-37 0.08431723781054501 1106 38-39 -0.03912422126656878 1106 40-41 -0.046629322593268796 1106 42-43 -0.05185865228008879 1106 44-45 0.14144522723361774 1106 46-47 0.13094715478035113 1106 48-49 0.19200952374441727 1106 50-51 0.44043597276941426 1106 52-53 0.26003456753910115 1106 54-55 -0.3841690857197051 1106 56-57 -0.2228949697375313 1106 58-59 -0.3034628783159974 1106 60-61 -0.23342984062780658 1106 62-63 -0.6794147310498353 1106 64-65 -0.4584969729560662 1106 66-67 -0.30010212500126343 1106 68-69 -0.16642126047732475 1106 70-71 -0.23958871737752574 1106 72-73 -0.031028216906594963 1106 74-75 -0.12864558173069796 1106 76-77 0.010117154080685253 1106 78-79 0.3414791760553335 1106 80-81 -0.14273544191393128 1106 82-83 0.19893658674812187 1106 84-85 0.23267856536591935 1106 86-87 -3.055375347749134E-4 1106 88-89 0.19331714283826074 1106 90-91 -0.1790425879124875 1106 92-93 -0.59617161568287 1106 94-95 -0.32675417623714154 1106 96-97 0.404054655616946 1106 98-99 0.5479757678850383 1106 100-101 1.088364236302894 1107 1 0.5298205895065458 1107 2 0.4871522085609534 1107 3 0.2583129303078202 1107 4 0.24438819969874004 1107 5 0.4291923153886259 1107 6 0.6046316610188356 1107 7 0.5108652606719062 1107 8 0.5644674894303066 1107 9 0.433611206880542 1107 10-11 0.488405169856307 1107 12-13 0.6437678778119746 1107 14-15 0.6599738693261017 1107 16-17 0.7963890250448387 1107 18-19 0.7002358116268965 1107 20-21 0.5852123310153914 1107 22-23 0.7300755209487093 1107 24-25 0.5531031157015249 1107 26-27 0.6671873246310795 1107 28-29 0.5488413040815985 1107 30-31 0.3739947902322527 1107 32-33 0.5574266418910483 1107 34-35 0.3492785182121665 1107 36-37 0.42412312587266143 1107 38-39 0.23379716339858447 1107 40-41 0.2902584160026507 1107 42-43 0.06499673432618636 1107 44-45 -0.23216849000999673 1107 46-47 -0.14700172907600262 1107 48-49 0.07481767644581083 1107 50-51 -0.2328089317922135 1107 52-53 -0.25808901706135146 1107 54-55 -0.2683683739759317 1107 56-57 -0.21898684938813062 1107 58-59 0.0934378010757797 1107 60-61 0.032438810294223686 1107 62-63 0.12802622656905882 1107 64-65 0.2236706103503039 1107 66-67 0.3186102658107721 1107 68-69 0.4976939126058184 1107 70-71 0.5379395906134157 1107 72-73 0.5059630480561985 1107 74-75 0.43180087572643444 1107 76-77 0.8580822139318691 1107 78-79 0.8449667205393148 1107 80-81 1.016138708846345 1107 82-83 0.9944526009829957 1107 84-85 1.559763651098688 1107 86-87 1.5147575488450364 1107 88-89 1.5400787086745638 1107 90-91 1.377029880544324 1107 92-93 1.912252842421303 1107 94-95 1.9778915694762205 1107 96-97 2.6153002072183718 1107 98-99 3.07212329295783 1107 100-101 3.295093450149544 1108 1 0.5811572640130684 1108 2 0.4007824712165693 1108 3 0.3505687147652061 1108 4 0.5552773868577958 1108 5 0.38824857762556775 1108 6 0.3142820692248165 1108 7 0.5467479830302722 1108 8 0.3393569721231131 1108 9 0.5992578716590771 1108 10-11 0.5951297939743228 1108 12-13 0.7412570723593674 1108 14-15 0.7574815347326407 1108 16-17 0.7219760905178703 1108 18-19 0.7707575518281047 1108 20-21 0.40065766342928555 1108 22-23 0.4800231868478093 1108 24-25 0.4163695024320546 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4111.0 3 549.0 4 438.0 5 346.0 6 334.0 7 384.0 8 491.0 9 555.0 10 634.0 11 1001.0 12 1395.0 13 1949.0 14 2484.0 15 3335.0 16 4148.0 17 5039.0 18 4479.0 19 2876.0 20 2810.0 21 2700.0 22 2640.0 23 2820.0 24 2922.0 25 3130.0 26 3462.0 27 3985.0 28 4552.0 29 5133.0 30 6158.0 31 7469.0 32 9474.0 33 12287.0 34 18260.0 35 29541.0 36 32427.0 37 46800.0 38 34341.0 39 2547.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.345263441988784 23.22810147839386 15.85187573095031 18.574759348667047 2 15.0368053274405 24.837250276226598 40.0345656523427 20.091378743990205 3 17.48096932318364 30.980709856378592 31.63143020342944 19.90689061700833 4 11.34914353130927 21.80485655829513 39.00031361538806 27.845686295007543 5 10.292722641749016 41.186576138511064 35.044927958410824 13.47577326132909 6 24.853885251840204 41.464844698228696 18.405790065317493 15.275479984613604 7 22.8855795686601 38.51931771091939 23.24583835766791 15.349264362752601 8 23.571681878417976 36.08638034977696 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16.138720123937247 32.60725518193951 35.150208584147606 16.10381610997564 34-35 16.657706996382533 32.29755811652798 34.78890643490986 16.255828452179628 36-37 16.886539595792176 31.602533951515966 34.555326599024944 16.955599853666914 38-39 16.299276665945076 32.16970357486769 34.97719519568836 16.553824563498875 40-41 16.351414900684496 31.855419618264197 35.42756798316003 16.365597497891272 42-43 16.271982861578884 31.55193777618536 34.67675116445718 17.499328197778574 44-45 16.318632618978757 31.439873860593565 34.64112779078772 17.60036572963996 46-47 16.910894710714803 31.26411340741045 34.37288882087496 17.45210306099978 48-49 16.619854468496477 31.259412984366282 34.10528358860756 18.01544895852968 50-51 16.477828283959187 31.272345253289746 34.054912410339085 18.19491405241198 52-53 16.68215439963576 31.43058136844384 34.08980645931764 17.79745777260276 54-55 17.107008839187248 30.796459846727707 33.681464577008406 18.41506673707663 56-57 17.09928962880446 31.058540781501616 33.18909457994428 18.653075009749646 58-59 16.837739045738925 30.275426219322192 33.868785837035595 19.018048897903284 60-61 16.947036661759025 30.761580073857203 33.64066736455937 18.65071589982441 62-63 16.869936145518057 30.61824275152732 33.83261243576299 18.679208667191634 64-65 17.004260115207845 30.354300514460693 33.47614578065725 19.165293589674214 66-67 17.07690700182481 30.60457583413254 32.89678437753057 19.421732786512074 68-69 16.809002517077502 29.933630506456893 33.60513043462036 19.652236541845248 70-71 17.23528599524554 29.626547344929634 33.531435779354304 19.60673088047052 72-73 17.140799289454144 29.07434981695234 33.54213081656796 20.24272007702556 74-75 16.910009945198784 29.65423174738822 33.05295211937224 20.38280618804076 76-77 17.12696564643366 29.255013471255104 33.36654501488928 20.251475867421952 78-79 16.880940740934246 28.78500069040413 33.445974943927986 20.888083624733635 80-81 17.328670754509254 29.159840118828583 32.68849669153459 20.82299243512758 82-83 17.496967774439735 28.563378179171877 32.699333843369224 21.240320203019163 84-85 16.992148140407966 28.390219508736315 32.92985572581187 21.68777662504385 86-87 17.280173137558535 28.469934140562327 32.92412171868878 21.325771003190358 88-89 17.80389624689045 27.97504520863154 33.053714759195216 21.167343785282792 90-91 17.852783863779724 27.74364640059122 32.913492510796175 21.49007722483288 92-93 18.188620380166554 27.55638403278083 32.69592833298813 21.55906725406448 94-95 17.826876456832956 27.06102788721197 33.233054081745 21.879041574210067 96-97 18.14481816553207 26.821890302161712 32.95413830168398 22.07915323062224 98-99 18.167517284492007 26.93096466592646 32.72082629668679 22.180691752894745 100-101 18.696762645710837 25.94012566771483 33.37462468774581 21.98848699882852 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5497.0 1 3195.0 2 1011.5 3 1058.0 4 756.0 5 689.5 6 1373.5 7 2392.0 8 3096.0 9 3122.5 10 2248.5 11 1394.5 12 1217.5 13 1167.5 14 1052.5 15 951.0 16 907.0 17 865.5 18 857.0 19 957.5 20 1078.0 21 1157.5 22 1348.5 23 1524.0 24 1641.5 25 1963.0 26 2419.5 27 2876.0 28 3184.5 29 3543.0 30 4235.5 31 5088.5 32 6127.0 33 7464.0 34 8809.0 35 9632.0 36 10683.0 37 12060.0 38 12388.5 39 11824.0 40 11095.5 41 10118.0 42 9119.0 43 9185.5 44 9332.5 45 8784.0 46 8080.0 47 7803.0 48 8297.5 49 7938.5 50 6616.5 51 5347.0 52 4407.0 53 3696.5 54 3055.5 55 2564.0 56 2149.0 57 1823.5 58 1548.5 59 1246.5 60 1014.0 61 880.0 62 723.5 63 565.0 64 439.5 65 335.5 66 297.0 67 255.5 68 200.5 69 181.5 70 158.0 71 117.0 72 104.0 73 99.0 74 80.5 75 61.5 76 47.0 77 36.0 78 24.5 79 20.0 80 17.5 81 12.0 82 7.5 83 6.5 84 4.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4589449489936792 2 0.041043857227077006 3 0.055595770243949766 4 0.06044640791624069 5 0.09104273784915264 6 0.08843085602561136 7 0.0533570143952001 8 0.04514824294978471 9 0.051118258546450454 10-11 0.05634202219353299 12-13 0.05130482153384625 14-15 0.04141698320186862 16-17 0.04421542801280568 18-19 0.05242419945822108 20-21 0.06175234882801131 22-23 0.05074513257165885 24-25 0.06678954948769804 26-27 0.05932702999186585 28-29 0.04664074684895114 30-31 0.047760124773325974 32-33 0.04794668776072177 34-35 0.051864510496033675 36-37 0.04626762087415953 38-39 0.02985007798332873 40-41 0.026865070184995857 42-43 0.02611881823541264 44-45 0.018096609777393042 46-47 0.03246195980686999 48-49 0.03414102669343223 50-51 0.019402550689163675 52-53 0.019402550689163675 54-55 0.01921598770176787 56-57 0.017723483802601435 58-59 0.01473847600426856 60-61 0.02108161757572592 62-63 0.019775676663955282 64-65 0.02070849160093431 66-67 0.0123131571681231 68-69 0.013619098079893734 70-71 0.01753692081520563 72-73 0.01604441691603919 74-75 0.013992224054685344 76-77 0.010447527294165055 78-79 0.01753692081520563 80-81 0.02126818056312172 82-83 0.019029424714372068 84-85 0.0164175428908308 86-87 0.0041043857227077 88-89 0.030409766945516146 90-91 0.03246195980686999 92-93 0.016604105878226607 94-95 0.032648522794265805 96-97 0.02611881823541264 98-99 0.02369349939926718 100-101 0.1475713230300814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 268006.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.008588751621055 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49800024506665 30.960664436197245 2 12.410117428506528 10.178428039698984 3 4.177908836486015 5.139904359516558 4 2.031231903627017 3.3319181518005085 5 1.1317834646323994 2.320642132849746 6 0.7429159826243182 1.8279561605068269 7 0.5223699211830867 1.4995157291809735 8 0.380347732738039 1.2478019003572574 9 0.3157961481711597 1.1655318932727368 >10 2.466781213260133 20.14062030548518 >50 0.23833633750497857 6.728472184345293 >100 0.07448010547030581 5.269319791383835 >500 0.003972272291749643 1.3414591075676912 >1k 0.003972272291749643 4.334289399466586 >5k 0.0019861361458748217 4.513476408370589 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5933 2.213756408438617 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5150 1.921598770176787 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4264 1.5910091565114213 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2892 1.0790803190973337 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2213 0.8257277822138311 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1274 0.47536249188451 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 987 0.3682753371193182 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 892 0.3328283695141153 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 775 0.28917263046349706 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 640 0.23880062386662984 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 479 0.17872734192518078 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 478 0.1783542159503892 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 414 0.15447415356372618 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 409 0.15260852368976813 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 402 0.14999664186622688 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 373 0.1391759885972702 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 372 0.1388028626224786 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 340 0.12686283142914712 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 316 0.11790780803414848 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 314 0.11716155608456527 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 275 0.1026096430676925 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.596889621874138E-4 0.0 22-23 0.0 0.0 0.0 0.005037200659686724 0.0 24-25 0.0 0.0 0.0 0.018096609777393045 0.0 26-27 0.0 0.0 0.0 0.044028865025409876 0.0 28-29 0.0 0.0 0.0 0.08488615926509108 0.0 30-31 0.0 0.0 0.0 0.18674955038320037 0.0 32-33 0.0 0.0 0.0 0.34476840070744685 0.0 34-35 0.0 0.0 0.0 0.5914046700447004 0.0 36-37 0.0 0.0 0.0 0.9453146571345419 0.0 38-39 0.0 0.0 0.0 1.3509025917330209 0.0 40-41 0.0 0.0 0.0 1.7064916457094244 0.0 42-43 0.0 0.0 0.0 2.0344693775512486 0.0 44-45 0.0 0.0 0.0 2.4643105005111825 0.0 46-47 0.0 0.0 0.0 2.9314642209502773 0.0 48-49 0.0 0.0 0.0 3.3512309425908375 0.0 50-51 0.0 0.0 0.0 3.7590576330380663 0.0 52-53 0.0 0.0 0.0 4.114273561039678 0.0 54-55 0.0 0.0 0.0 4.536838727491176 0.0 56-57 0.0 0.0 0.0 5.022648746669851 0.0 58-59 0.0 0.0 0.0 5.488123400222383 0.0 60-61 0.0 0.0 0.0 5.945575845316895 0.0 62-63 0.0 0.0 0.0 6.359559114348187 0.0 64-65 0.0 0.0 0.0 6.818317500354469 0.0 66-67 0.0 0.0 0.0 7.350581703394701 0.0 68-69 0.0 0.0 0.0 7.920158503914092 0.0 70-71 0.0 0.0 0.0 8.507458788236086 0.0 72-73 0.0 0.0 0.0 9.026850145146003 0.0 74-75 0.0 0.0 0.0 9.581315343686335 0.0 76-77 0.0 0.0 0.0 10.171973761781452 0.0 78-79 0.0 0.0 0.0 10.80218353320448 0.0 80-81 0.0 0.0 0.0 11.46616120534615 0.0 82-83 0.0 0.0 0.0 12.082378752714492 0.0 84-85 0.0 0.0 0.0 12.69430535137273 0.0 86-87 0.0 0.0 0.0 13.336641716976485 0.0 88-89 0.0 0.0 0.0 14.021514443706483 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 60 3.255991E-10 63.37256 9 GGTATCA 2070 0.0 38.96435 1 GTATTGA 50 0.0015764674 38.180454 1 ATTGATC 55 0.002565942 34.57331 3 TGTAAGG 55 0.002565942 34.57331 2 GTTCAGG 70 1.9928762E-4 34.08969 1 GTATCAA 3130 0.0 31.867872 1 CTGTACT 75 3.057696E-4 31.692202 4 ATTAAGC 65 0.0058132266 29.254341 3 CTTTAGG 120 5.327962E-6 27.730677 2 CAGGACT 70 0.008347386 27.164747 4 GCATGGC 70 0.008354999 27.15967 8 CTTTCCG 35 0.008392868 27.139393 34-35 GTACATG 1820 0.0 26.485067 1 ATCAACG 3850 0.0 25.43608 3 TATCAAC 3870 0.0 25.427465 2 CATGGGG 975 0.0 25.353762 4 GTAGGGG 150 1.0476433E-6 25.353762 6 ACATGGG 1715 0.0 25.224405 3 AGGGGTG 170 1.109438E-7 25.162636 8 >>END_MODULE