##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938202_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 268006 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43551636903651 33.0 31.0 34.0 31.0 34.0 2 32.64837727513563 34.0 31.0 34.0 31.0 34.0 3 32.56617389162929 34.0 31.0 34.0 31.0 34.0 4 36.138052879413145 37.0 35.0 37.0 35.0 37.0 5 36.11673992373305 37.0 35.0 37.0 35.0 37.0 6 36.202618598091085 37.0 37.0 37.0 35.0 37.0 7 36.157123347984744 37.0 37.0 37.0 35.0 37.0 8 36.17517518264516 37.0 37.0 37.0 35.0 37.0 9 37.84295500846996 39.0 38.0 39.0 35.0 39.0 10-11 37.83997373193138 39.0 38.0 39.0 35.0 39.0 12-13 37.79998208995321 39.0 38.0 39.0 35.0 39.0 14-15 38.979994850861544 40.0 38.0 41.0 35.5 41.0 16-17 38.91781713842228 40.0 38.0 41.0 35.5 41.0 18-19 38.95317082453378 40.0 38.0 41.0 35.0 41.0 20-21 39.000300366409704 40.0 38.0 41.0 35.0 41.0 22-23 38.92277784825713 40.0 38.0 41.0 35.0 41.0 24-25 38.83472571509593 40.0 38.0 41.0 35.0 41.0 26-27 38.58191421087588 40.0 38.0 41.0 35.0 41.0 28-29 38.426042700536556 40.0 38.0 41.0 34.5 41.0 30-31 38.076449780975054 40.0 37.0 41.0 34.0 41.0 32-33 37.693454624150206 40.0 35.0 41.0 33.5 41.0 34-35 37.37786654030134 40.0 35.0 41.0 33.0 41.0 36-37 37.16447019842839 40.0 35.0 41.0 33.0 41.0 38-39 36.938658089744266 40.0 35.0 41.0 33.0 41.0 40-41 36.74895897853033 40.0 35.0 41.0 33.0 41.0 42-43 36.43996403065603 40.0 35.0 41.0 31.5 41.0 44-45 36.04943546040014 40.0 35.0 41.0 30.5 41.0 46-47 35.498522421139825 39.0 35.0 41.0 28.0 41.0 48-49 34.99211398252278 39.0 35.0 41.0 24.0 41.0 50-51 34.62221554741312 39.0 35.0 41.0 22.5 41.0 52-53 34.19899367924599 39.0 35.0 40.0 19.0 41.0 54-55 33.85802370096192 38.0 35.0 40.0 11.0 41.0 56-57 33.55928038924502 38.0 34.0 40.0 4.5 41.0 58-59 33.16322022641285 38.0 33.5 40.0 2.0 41.0 60-61 32.69666910442304 37.5 33.0 40.0 2.0 41.0 62-63 32.24314567584308 37.0 33.0 40.0 2.0 41.0 64-65 31.776825891957643 36.5 32.5 40.0 2.0 41.0 66-67 31.203504772281217 36.0 31.5 39.0 2.0 41.0 68-69 30.601837272299875 35.0 31.0 39.0 2.0 40.0 70-71 30.059157257673334 35.0 30.0 38.5 2.0 40.0 72-73 29.616960068058177 35.0 29.5 37.5 2.0 40.0 74-75 29.104036849921272 35.0 29.0 37.0 2.0 39.0 76-77 27.85418796594106 33.5 27.0 35.5 2.0 38.5 78-79 28.47894823250226 34.0 29.0 36.0 2.0 38.5 80-81 28.31522988291307 34.0 29.0 36.0 2.0 37.5 82-83 27.936639478220634 34.0 28.5 35.0 2.0 37.0 84-85 27.499468295485922 34.0 26.5 35.0 2.0 36.5 86-87 27.07089953210003 34.0 26.0 35.0 2.0 36.0 88-89 26.57266814922054 34.0 25.0 35.0 2.0 36.0 90-91 26.168016760818787 33.0 24.0 35.0 2.0 35.0 92-93 25.708454288336828 33.0 21.5 35.0 2.0 35.0 94-95 25.16301687275658 33.0 18.5 35.0 2.0 35.0 96-97 24.578817265285107 32.0 4.5 35.0 2.0 35.0 98-99 23.873161421759214 32.0 2.0 34.5 2.0 35.0 100-101 20.90123728573241 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13501463711619976 1101 2 0.2336501318551285 1101 3 0.2773850712698902 1101 4 0.12418297512222409 1101 5 0.09375873865181461 1101 6 0.18849248801887342 1101 7 0.27117571185006994 1101 8 0.1605837651133939 1101 9 0.27188135768795263 1101 10-11 0.22096027003099294 1101 12-13 0.15778301846156495 1101 14-15 0.2622696488785863 1101 16-17 0.08835426779456412 1101 18-19 0.11342193680010126 1101 20-21 -0.1520095785129172 1101 22-23 -0.3336701632822354 1101 24-25 0.007811061676271436 1101 26-27 -0.004367988873170248 1101 28-29 -0.06738127227558977 1101 30-31 -0.005298516390389807 1101 32-33 -0.21763308936874637 1101 34-35 -0.3072593656172842 1101 36-37 -0.4652172626637352 1101 38-39 -0.4115418442930121 1101 40-41 -0.524044233691427 1101 42-43 -0.4074250843322531 1101 44-45 -0.22827599478026883 1101 46-47 0.1753619808760405 1101 48-49 0.30000926565111996 1101 50-51 0.5808549809227657 1101 52-53 0.5630224508024355 1101 54-55 0.46408979145601137 1101 56-57 0.39347611103023183 1101 58-59 0.6206607791562888 1101 60-61 0.18673716373277927 1101 62-63 0.46789422744944176 1101 64-65 0.6381387363569466 1101 66-67 0.46138680591828773 1101 68-69 0.7851889411742086 1101 70-71 0.9604805024485827 1101 72-73 1.234177865707963 1101 74-75 0.8885472084330495 1101 76-77 1.2969474099140648 1101 78-79 1.1492705736402264 1101 80-81 1.312688623564643 1101 82-83 1.3274354493742457 1101 84-85 1.5651328654749115 1101 86-87 1.5436390862738456 1101 88-89 1.8443208045636474 1101 90-91 2.1948530046101133 1101 92-93 1.8128791195772074 1101 94-95 1.6235917997508942 1101 96-97 1.4094378329107577 1101 98-99 1.3710477505360252 1101 100-101 1.9875768793461859 1102 1 0.10368200887717194 1102 2 0.0639647801893446 1102 3 0.13566111569083716 1102 4 -0.010666310969789095 1102 5 -0.07890122961894264 1102 6 0.07625028812463341 1102 7 0.0857579434735527 1102 8 0.04715171857716882 1102 9 -0.0783301706039623 1102 10-11 -0.011324235521620096 1102 12-13 0.14505826965141466 1102 14-15 0.1108616636855686 1102 16-17 0.012732903437076004 1102 18-19 0.3890036396028549 1102 20-21 0.16128365258174426 1102 22-23 0.33013205776482835 1102 24-25 0.35134622825480477 1102 26-27 0.2611000174726783 1102 28-29 -0.12089131986945034 1102 30-31 0.32167795108713904 1102 32-33 0.2168922940262874 1102 34-35 -0.12964170300490707 1102 36-37 -0.08899436472614042 1102 38-39 -0.2125069421248469 1102 40-41 -0.018756026393702996 1102 42-43 -0.3624422710059747 1102 44-45 -0.27236642312505666 1102 46-47 -0.1944820170086743 1102 48-49 -0.31391855031926497 1102 50-51 -0.33179044477792274 1102 52-53 -0.5578461372462158 1102 54-55 -0.17442818844880037 1102 56-57 -0.49649612588145686 1102 58-59 -0.2604206592360967 1102 60-61 -0.1597395681551106 1102 62-63 -0.32798097085833433 1102 64-65 -0.1565466153088373 1102 66-67 -0.18598892121021393 1102 68-69 0.02104298665279103 1102 70-71 0.5157422687098183 1102 72-73 0.26795628982218744 1102 74-75 0.27465244375827425 1102 76-77 0.3007483089093057 1102 78-79 0.5482988655492669 1102 80-81 0.34749163784280057 1102 82-83 0.4337945186497656 1102 84-85 0.6195683493458795 1102 86-87 0.8690951941532781 1102 88-89 0.7461254053039994 1102 90-91 0.8559780707127054 1102 92-93 0.9525869461240042 1102 94-95 1.3891589599835754 1102 96-97 1.4056699852111265 1102 98-99 1.5887566347242839 1102 100-101 2.307050702720062 1103 1 0.3401193926865389 1103 2 0.31899266594226106 1103 3 0.18027832035536306 1103 4 0.19019561230214066 1103 5 0.03581061760093007 1103 6 0.24918420734045554 1103 7 0.2543399951889498 1103 8 0.09282520176121523 1103 9 0.2739598412263575 1103 10-11 0.3284594386375801 1103 12-13 0.06543599484999874 1103 14-15 0.13217736420948256 1103 16-17 0.13038120494122296 1103 18-19 0.24108239572926493 1103 20-21 0.03864971646547133 1103 22-23 0.08032218789833934 1103 24-25 0.1694339416230619 1103 26-27 0.3007949642362391 1103 28-29 0.19802029074403293 1103 30-31 0.014343153047590818 1103 32-33 -0.06388766203253482 1103 34-35 0.048784864512420256 1103 36-37 0.12126843736951542 1103 38-39 0.29913324716026324 1103 40-41 0.012911203621506218 1103 42-43 -0.04055195496744801 1103 44-45 0.12361126827260449 1103 46-47 -0.05023662238301796 1103 48-49 -0.17307437252646451 1103 50-51 0.17104038165439306 1103 52-53 -0.1709524411164196 1103 54-55 -0.24352992919579464 1103 56-57 -0.27182568477954305 1103 58-59 -0.2287323036504958 1103 60-61 0.12336420064665532 1103 62-63 0.27401328298652317 1103 64-65 0.15647096116572712 1103 66-67 0.035069047354937766 1103 68-69 0.3285045381146787 1103 70-71 0.8846158532966051 1103 72-73 0.66596203597733 1103 74-75 0.6441175443159857 1103 76-77 0.5895771072868641 1103 78-79 1.128132396054589 1103 80-81 0.8881583608443222 1103 82-83 0.8498415019183128 1103 84-85 0.7832281431603114 1103 86-87 0.6676206293400249 1103 88-89 1.0777968604332884 1103 90-91 1.5672549001870983 1103 92-93 1.7984336589852532 1103 94-95 1.4587888399903335 1103 96-97 1.4872146869180725 1103 98-99 2.1784346810315363 1103 100-101 2.9958076741582893 1104 1 0.05922008884813579 1104 2 -0.001336774465549695 1104 3 0.17531388926774838 1104 4 0.1267421942628033 1104 5 0.07527879578786667 1104 6 0.03618919079234928 1104 7 0.04199168613885007 1104 8 -0.14054110097397654 1104 9 -0.19362870063844184 1104 10-11 0.018543891028492965 1104 12-13 -0.10936523769841244 1104 14-15 0.08302759436126905 1104 16-17 0.026441995446027988 1104 18-19 0.06216315808327977 1104 20-21 0.0037084907645521525 1104 22-23 -0.018871329810032478 1104 24-25 -0.22689314020446005 1104 26-27 0.05069986019241668 1104 28-29 -0.12384032476933982 1104 30-31 -0.01497742366333199 1104 32-33 -0.0816150558013149 1104 34-35 0.11913332572898838 1104 36-37 -0.4637070257871798 1104 38-39 -0.5125417252595668 1104 40-41 -0.7345400080040179 1104 42-43 -0.9641073245417573 1104 44-45 -1.014774625895619 1104 46-47 -1.2023460300750344 1104 48-49 -1.2753017092328207 1104 50-51 -1.375541579010573 1104 52-53 -1.4454512428054684 1104 54-55 -1.4433245044611382 1104 56-57 -1.6217229724122149 1104 58-59 -1.3953640988344205 1104 60-61 -1.5268906781907958 1104 62-63 -1.0086264248538583 1104 64-65 -1.1152983544655761 1104 66-67 -0.920926311567726 1104 68-69 -1.1941587555285658 1104 70-71 -0.9893617782322899 1104 72-73 -0.963759879798527 1104 74-75 -1.129278050464702 1104 76-77 -0.7711620413417393 1104 78-79 -0.8252190704497657 1104 80-81 -0.5630627750783752 1104 82-83 -0.5082537542919816 1104 84-85 -0.4297858941965558 1104 86-87 -0.5573384759211315 1104 88-89 -0.7621620546197825 1104 90-91 -0.646691156799239 1104 92-93 -0.725903465829294 1104 94-95 -0.5312632770256798 1104 96-97 -0.1513138633158846 1104 98-99 0.3011379183731897 1104 100-101 0.5859282660858582 1105 1 0.06601822134559399 1105 2 0.16958203149670936 1105 3 0.15014492790071898 1105 4 0.2514231184914024 1105 5 0.2666978067521697 1105 6 0.17236345576080936 1105 7 0.17216137493250727 1105 8 0.18477731350048998 1105 9 0.14553727166644137 1105 10-11 0.08296743848977428 1105 12-13 -0.020308379387891762 1105 14-15 -0.02334863069130222 1105 16-17 0.19812129288416713 1105 18-19 0.11924630955995497 1105 20-21 0.22187034980249365 1105 22-23 0.17663449621597493 1105 24-25 0.14669995056515717 1105 26-27 0.022065702883104166 1105 28-29 0.009007616613295966 1105 30-31 0.05675345493577311 1105 32-33 -0.03192520406408761 1105 34-35 0.16182665588809897 1105 36-37 0.12013219970184963 1105 38-39 0.1063793026603932 1105 40-41 0.4772998523300771 1105 42-43 0.7670820841265211 1105 44-45 0.7408100267251072 1105 46-47 0.8371003321305253 1105 48-49 0.6411831007765727 1105 50-51 0.7141435113887127 1105 52-53 0.8504328450676724 1105 54-55 0.514941045936304 1105 56-57 0.2131445698115897 1105 58-59 0.2823991304107736 1105 60-61 0.07450598093708294 1105 62-63 -0.07780469728174211 1105 64-65 -0.23417309160004152 1105 66-67 -0.28301283565877355 1105 68-69 -0.44240962513403304 1105 70-71 -0.030110895400877524 1105 72-73 0.2211850341667443 1105 74-75 -0.09801193135189834 1105 76-77 -0.02316907754113018 1105 78-79 0.11051609335348545 1105 80-81 0.31873701066141535 1105 82-83 0.4300340156824909 1105 84-85 0.7759303565143405 1105 86-87 0.8153684769548484 1105 88-89 0.7621075429149045 1105 90-91 0.9056504956495424 1105 92-93 0.9376551052402888 1105 94-95 0.8670953839802849 1105 96-97 1.5075561124806498 1105 98-99 1.6391929118263455 1105 100-101 2.164100176837227 1106 1 -0.11551322212133641 1106 2 0.010820219831671807 1106 3 0.16072817977428855 1106 4 -0.08819239144668956 1106 5 0.04692589407703451 1106 6 -0.014733318432739395 1106 7 0.04984140099963241 1106 8 -0.024606850722378226 1106 9 0.0729664016762186 1106 10-11 0.07304238146794972 1106 12-13 0.06399269588091983 1106 14-15 -0.07093416343813175 1106 16-17 0.038075675419193544 1106 18-19 -0.17978920689491673 1106 20-21 -0.06016940255983627 1106 22-23 -0.1578783744259269 1106 24-25 -0.048288645068595315 1106 26-27 -0.014734557788123936 1106 28-29 -0.12099564177704991 1106 30-31 -0.3049539416103286 1106 32-33 -0.23640874919279753 1106 34-35 -0.23193678497212034 1106 36-37 -0.14481403685729077 1106 38-39 -0.5003981492783538 1106 40-41 -0.3598066970345428 1106 42-43 -0.45774033359912636 1106 44-45 0.04669467970653329 1106 46-47 -0.5959723299743942 1106 48-49 -0.44766668742805393 1106 50-51 -0.5718649604262112 1106 52-53 -0.5999027691389074 1106 54-55 -0.39994979798679964 1106 56-57 -0.4445810737204994 1106 58-59 -0.7460987516354933 1106 60-61 -0.6181821911059231 1106 62-63 -0.5955368575945492 1106 64-65 -0.6210548120301489 1106 66-67 -0.524602930152092 1106 68-69 -0.9782714693829746 1106 70-71 -0.7191236031232648 1106 72-73 -0.8381241870779625 1106 74-75 -0.6606903282238434 1106 76-77 -0.6808165197047025 1106 78-79 -0.3032734146295688 1106 80-81 -0.1757497928576477 1106 82-83 -0.5504677764322032 1106 84-85 -0.4108861961086667 1106 86-87 -0.12827440346222474 1106 88-89 0.03924015940235748 1106 90-91 -0.7010487549206772 1106 92-93 -0.8365710270503577 1106 94-95 -0.33373224716992667 1106 96-97 -0.6016325483209144 1106 98-99 -0.8310123667660285 1106 100-101 -0.6644172555511751 1107 1 0.10858253976802246 1107 2 0.05331779343244847 1107 3 0.1556424470017248 1107 4 0.09198230929740703 1107 5 0.007689401032713761 1107 6 0.07708162818647679 1107 7 0.030947839045573744 1107 8 -0.13803292642603537 1107 9 -0.14991292540239698 1107 10-11 0.13853574931006563 1107 12-13 0.13772935068519843 1107 14-15 0.42342364956736844 1107 16-17 0.2737663903981655 1107 18-19 0.2522263220601175 1107 20-21 0.24688980158121865 1107 22-23 0.14014168380764858 1107 24-25 0.04015522422937323 1107 26-27 0.09434341050691586 1107 28-29 -0.04570609146971094 1107 30-31 -0.13636125315998981 1107 32-33 0.046781156986433814 1107 34-35 -0.011153866822652958 1107 36-37 -0.37833716852988175 1107 38-39 -0.2141477448784741 1107 40-41 -0.21094872226909445 1107 42-43 -0.4440813235700034 1107 44-45 -0.4835997233992586 1107 46-47 -0.2632644684409158 1107 48-49 -0.4683654904044374 1107 50-51 -0.08362814386198636 1107 52-53 0.06545472034669331 1107 54-55 -0.12634902153904903 1107 56-57 -0.12337757970561825 1107 58-59 -0.3326597351359837 1107 60-61 -0.39170532277852743 1107 62-63 -0.075355686452518 1107 64-65 0.17488824846807205 1107 66-67 0.07897519990290647 1107 68-69 0.30912419545041203 1107 70-71 0.35241311638498374 1107 72-73 0.39166552498932816 1107 74-75 0.29030934825626176 1107 76-77 0.4000569840157766 1107 78-79 0.12260170669038928 1107 80-81 0.14522076683177332 1107 82-83 0.2087493054183298 1107 84-85 0.3931448618013924 1107 86-87 0.826937502069967 1107 88-89 0.6994536825340312 1107 90-91 1.2470068905015133 1107 92-93 1.0504881770906955 1107 94-95 0.6886747408598382 1107 96-97 0.47468579525721566 1107 98-99 0.7011260997496613 1107 100-101 1.6905099524721159 1108 1 0.12415963097712535 1108 2 -0.0807683300267783 1108 3 0.12150829930873641 1108 4 0.14410707379395404 1108 5 0.09054967514025947 1108 6 -0.09362605199675755 1108 7 0.015050586278022138 1108 8 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-0.720660344896487 2316 54-55 -1.0979801010171073 2316 56-57 -0.969884104023528 2316 58-59 -1.098371478908227 2316 60-61 -1.308182191105928 2316 62-63 -1.2387186757763686 2316 64-65 -1.0377214786968167 2316 66-67 -0.7003605059096643 2316 68-69 -0.5935744996860066 2316 70-71 -1.2371539061535692 2316 72-73 -1.3046393385931125 2316 74-75 -0.8405388130723317 2316 76-77 -0.9665740954622777 2316 78-79 -1.1731218994780548 2316 80-81 -1.0786285807364315 2316 82-83 -1.268801109765537 2316 84-85 -1.0987649839874543 2316 86-87 -1.410850161037981 2316 88-89 -1.4924265072643088 2316 90-91 -1.318776027647953 2316 92-93 -1.4509649664442996 2316 94-95 -1.2776716411093219 2316 96-97 -1.8507234574118208 2316 98-99 -2.150860851614514 2316 100-101 -2.880326346460265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 5.0 5 16.0 6 39.0 7 79.0 8 161.0 9 263.0 10 477.0 11 788.0 12 1261.0 13 1768.0 14 2334.0 15 3071.0 16 3824.0 17 4998.0 18 4738.0 19 3307.0 20 2801.0 21 2854.0 22 2888.0 23 3127.0 24 3367.0 25 3477.0 26 3277.0 27 3059.0 28 3575.0 29 4207.0 30 5107.0 31 6138.0 32 7578.0 33 9960.0 34 14519.0 35 27204.0 36 31055.0 37 53766.0 38 48885.0 39 4029.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.87072677477369 23.792004656612164 15.972776728879204 19.36449183973493 2 14.524278655978984 24.850730673353933 40.31876464705268 20.306226023614407 3 17.37803285295281 30.95133255218634 32.20776022270152 19.462874372159327 4 10.813190749460833 22.474496839622994 38.195413535517865 28.51689887539831 5 9.782653308706928 41.70556519467921 35.4291897539225 13.082591742691369 6 24.414005656589776 41.727424012895234 17.986164488854726 15.872405841660262 7 22.071893912822848 38.60361335193988 22.911427356103967 16.413065379133304 8 23.2834339529712 36.4062744863921 21.74540868488019 18.564882875756513 9 24.730416483213062 18.774206547614604 22.543525144959442 33.951851824212895 10-11 22.001932792549418 30.74184906308068 27.482966799250764 19.77325134511914 12-13 23.685290627821765 30.021342805758078 28.21895778452721 18.07440878189294 14-15 20.787780870577524 31.808243099035096 27.798444810937067 19.605531219450313 16-17 16.949807093871033 34.91563621709962 29.274344604225277 18.860212084804072 18-19 16.268292500914157 32.01085050334694 34.10893785960016 17.61191913613874 20-21 17.513973567755944 30.99464191100199 34.54885338387946 16.942531137362597 22-23 17.510988559957614 31.483810063953793 34.33020156265158 16.674999813437015 24-25 17.16601274997621 32.45385736220641 34.25739877350975 16.122731114307623 26-27 16.323205858148167 32.816602565179174 34.762040722639306 16.098150854033356 28-29 15.815435590513575 32.10623722033999 35.44200832823391 16.636318860912525 30-31 16.642351290642747 31.841078184816755 35.25779273598352 16.258777788556973 32-33 15.58486384023523 33.17107845698038 34.92242363635007 16.321634066434324 34-35 16.07282470979648 32.732120832143785 35.18772496487329 16.007329493186447 36-37 16.64580072057482 31.846160448241566 34.459410292913105 17.048628538270506 38-39 15.837471378906884 32.16889570632286 34.87602658052814 17.117606334242115 40-41 15.964573761742498 31.71249211568391 35.633936335780426 16.688997786793163 42-43 16.28793109239307 31.28832295215952 35.12351046638776 17.30023548905965 44-45 15.81845321391921 31.63093572161582 34.862767049165036 17.687844015299934 46-47 16.88439135966925 31.00160821495603 34.14247814357516 17.971522281799558 48-49 16.50877219166735 30.854906233442534 34.12703446937755 18.509287105512563 50-51 15.964194831458997 30.866846264635868 34.45799720901771 18.71096169488743 52-53 16.416423512906427 31.139974478183323 34.57217375730394 17.871428251606307 54-55 16.694215801138782 30.81255643530369 33.805399879107185 18.687827884450346 56-57 16.977604978993007 31.297620202532777 32.8266904472288 18.898084371245417 58-59 16.20075669947688 31.323179331806006 33.649806347619084 18.826257621098037 60-61 16.663619471205866 32.40281933986552 32.37408863980657 18.559472549122034 62-63 16.098520182383975 32.714939217778706 32.45468385036156 18.73185674947576 64-65 16.195906061804585 32.218121982343675 32.37017081707126 19.215801138780474 66-67 16.444438640251786 32.29224773372189 31.63144039954404 19.631873226482295 68-69 16.11627373318831 31.61390344601137 32.19784668598219 20.07197613481813 70-71 16.73525965836586 31.064416468288027 31.99387327149392 20.206450601852197 72-73 16.61063558278546 30.32842548301157 32.16401871599889 20.89692021820407 74-75 16.4621314448184 30.950239920001792 31.392767326104636 21.19486130907517 76-77 16.272396886636866 30.58998679134049 32.253942075923675 20.88367424609897 78-79 16.236763356044268 30.055856958426304 31.916076505749867 21.791303179779558 80-81 16.47279626724078 30.33146707810101 31.44991427414736 21.745822380510848 82-83 16.564149348795816 29.749051784771467 31.52160326264396 22.165195603788757 84-85 16.16733397763358 29.620957420211873 31.63651018131206 22.57519842084249 86-87 16.36943685226817 29.923788024365443 31.540937118123896 22.165838005242488 88-89 16.51324970336485 29.43590815131005 31.320194324007673 22.730647821317433 90-91 16.512311704981773 28.99696638395206 31.560614484490497 22.93010742657567 92-93 16.681032070297196 29.122777560120145 31.28113281468629 22.915057554896364 94-95 16.332470168578315 28.770064849294418 31.771863316492915 23.125601665634353 96-97 16.501496235158914 28.829765005261077 31.39780452676433 23.270934232815684 98-99 16.20224920337604 29.03050677969896 31.161429221733844 23.60581479519115 100-101 16.269086105762774 28.44650666427857 31.93517813698712 23.349229092971537 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5331.0 1 3075.0 2 922.0 3 982.5 4 715.0 5 698.5 6 1433.5 7 2440.0 8 3124.0 9 3108.0 10 2243.5 11 1362.5 12 1130.5 13 1125.5 14 1046.5 15 889.0 16 795.5 17 795.5 18 845.5 19 924.0 20 989.0 21 1070.5 22 1194.0 23 1334.5 24 1475.5 25 1744.0 26 2238.0 27 2754.0 28 2982.0 29 3379.0 30 4213.0 31 4997.0 32 5896.5 33 7394.0 34 8906.5 35 9905.0 36 11057.5 37 12214.5 38 12369.5 39 11980.5 40 11417.0 41 10370.5 42 9383.5 43 9752.0 44 10060.5 45 9483.5 46 8740.5 47 8305.5 48 8811.5 49 8425.5 50 7115.5 51 5805.0 52 4469.0 53 3375.0 54 2717.0 55 2355.0 56 1993.0 57 1626.0 58 1293.0 59 1023.0 60 792.5 61 613.5 62 459.5 63 333.0 64 252.5 65 161.5 66 98.0 67 75.0 68 57.5 69 40.5 70 32.0 71 30.0 72 27.0 73 28.5 74 22.0 75 14.5 76 10.5 77 5.0 78 2.5 79 2.0 80 3.0 81 3.5 82 1.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.012686283142914713 3 0.00895502339499862 4 0.0 5 3.7312597479160917E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00205219286135385 26-27 0.02667850719760005 28-29 7.462519495832183E-4 30-31 0.0 32-33 0.00447751169749931 34-35 0.017723483802601435 36-37 0.009514712357186034 38-39 0.0244397513488504 40-41 0.025745692260621032 42-43 0.019775676663955282 44-45 0.011566905218539883 46-47 0.0026118818235412638 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.8656298739580458E-4 68-69 1.8656298739580458E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8656298739580458E-4 82-83 9.328149369790228E-4 84-85 0.005596889621874137 86-87 0.0013059409117706319 88-89 0.0 90-91 0.003358133773124482 92-93 3.7312597479160917E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0037312597479160913 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 268006.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.71733435497919 #Duplication Level Percentage of deduplicated Percentage of total 1 75.18827620027089 29.86277905420683 2 12.449769449865244 9.889433117654063 3 4.34455300023354 5.1766219239961035 4 2.060857309177033 3.2740703522594767 5 1.1617461485833533 2.3070730109447184 6 0.702347444459436 1.6737220970976352 7 0.4791809552336148 1.3322253150886256 8 0.4074519099877275 1.2946322994045962 9 0.3426977492299214 1.2249936979977238 >10 2.5054789081917828 19.737985730576067 >50 0.25901571153073155 7.021951133834281 >100 0.08567442766016505 5.984505037428495 >500 0.004981071375590991 1.5308462803180727 >1k 0.006973499925827387 7.4405697341383705 >5k 9.962142751181982E-4 2.2485912150549514 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5683 2.120474914740715 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4992 1.8626448661597128 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4329 1.615262344872876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3004 1.1208704282739939 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2020 0.7537144690790505 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1921 0.7167749975746812 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1357 0.5063319477922136 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1182 0.441034902203682 TruSeq Adapter, Index 11 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 925 0.34514152668223846 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 871 0.32499272404349155 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 747 0.278725103169332 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 680 0.25372566285829423 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 646 0.2410393797153795 RNA PCR Primer, Index 11 (95% over 23bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 498 0.18581673544622135 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 462 0.17238420035372343 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 443 0.16529480683268286 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 443 0.16529480683268286 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 424 0.15820541331164226 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 394 0.147011634067894 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 356 0.13283284702581286 TruSeq Adapter, Index 11 (96% over 26bp) TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 334 0.12462407558039745 TruSeq Adapter, Index 11 (96% over 28bp) ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 331 0.12350469765602262 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 310 0.11566905218539883 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 303 0.11305717036185757 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 303 0.11305717036185757 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 294 0.10969903658873308 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 283 0.10559465086602537 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.596889621874138E-4 0.0 22-23 0.0 0.0 0.0 0.004850637672290919 0.0 24-25 0.0 0.0 0.0 0.017350357827809824 0.0 26-27 0.0 0.0 0.0 0.04290948710103505 0.0 28-29 0.0 0.0 0.0 0.08358021835332044 0.0 30-31 0.0 0.0 0.0 0.18581673544622135 0.0 32-33 0.0 0.0 0.0 0.34514152668223846 0.0 34-35 0.0 0.0 0.0 0.5930837369312627 0.0 36-37 0.0 0.0 0.0 0.9494190428572494 0.0 38-39 0.0 0.0 0.0 1.3591113631784362 0.0 40-41 0.0 0.0 0.0 1.7171257359909853 0.0 42-43 0.0 0.0 0.0 2.0488347275807257 0.0 44-45 0.0 0.0 0.0 2.479235539502847 0.0 46-47 0.0 0.0 0.0 2.945269882017567 0.0 48-49 0.0 0.0 0.0 3.36802161145646 0.0 50-51 0.0 0.0 0.0 3.78069893957598 0.0 52-53 0.0 0.0 0.0 4.138153623426341 0.0 54-55 0.0 0.0 0.0 4.559599411953464 0.0 56-57 0.0 0.0 0.0 5.046715372043909 0.0 58-59 0.0 0.0 0.0 5.5190928561300865 0.0 60-61 0.0 0.0 0.0 5.977291553174183 0.0 62-63 0.0 0.0 0.0 6.393327015066827 0.0 64-65 0.0 0.0 0.0 6.852271964060506 0.0 66-67 0.0 0.0 0.0 7.386215233987299 0.0 68-69 0.0 0.0 0.0 7.963441116989918 0.0 70-71 0.0 0.0 0.0 8.555032350022014 0.0 72-73 0.0 0.0 0.0 9.07946090759162 0.0 74-75 0.0 0.0 0.0 9.637097676917682 0.0 76-77 0.0 0.0 0.0 10.232606732685088 0.0 78-79 0.0 0.0 0.0 10.869719334641761 0.0 80-81 0.0 0.0 0.0 11.546383289926347 0.0 82-83 0.0 0.0 0.0 12.176406498361978 0.0 84-85 0.0 0.0 0.0 12.800459691200944 0.0 86-87 0.0 0.0 0.0 13.453989836048446 0.0 88-89 0.0 0.0 0.0 14.154906979694484 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCTAC 40 5.378085E-4 47.504757 1 GGTATCA 2325 0.0 36.777878 1 CCCTATA 90 2.4044664E-5 31.66984 2 TATGGGT 65 0.005838649 29.228243 3 GTAGCCC 105 6.890472E-5 27.140512 3 CACCCGC 35 0.008391181 27.140512 86-87 GTATCAA 3225 0.0 26.956188 1 CCTATAC 125 7.3682168E-6 26.5977 3 GTGGAAC 90 8.9340884E-4 26.386608 7 GTAGGAT 110 9.4556235E-5 25.906853 3 TCCCATC 110 9.4556235E-5 25.906853 8 GTATAGT 190 1.1810698E-8 25.002502 1 CTATACA 115 1.2789227E-4 24.780466 4 GGATGAC 135 1.3386323E-5 24.6275 8 GTACATG 1865 0.0 24.45285 1 GTAGGGG 195 1.5597834E-8 24.356869 6 CACGAGC 60 1.7079961E-4 23.752378 94-95 CTAGATG 120 1.7070514E-4 23.747948 3 AGTACAT 1425 0.0 23.335672 2 ACATGGG 1835 0.0 23.294989 3 >>END_MODULE