##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938201_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 274640 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.716206670550537 33.0 31.0 34.0 30.0 34.0 2 31.858603990678706 34.0 31.0 34.0 30.0 34.0 3 31.889728371686573 34.0 31.0 34.0 30.0 34.0 4 35.37835348092048 37.0 35.0 37.0 35.0 37.0 5 35.41291872997378 37.0 35.0 37.0 35.0 37.0 6 35.49022720652491 37.0 37.0 37.0 35.0 37.0 7 35.434940285464606 37.0 36.0 37.0 35.0 37.0 8 35.43413923681911 37.0 36.0 37.0 35.0 37.0 9 37.08660064083892 39.0 38.0 39.0 35.0 39.0 10-11 37.04783898922226 39.0 38.0 39.0 35.0 39.0 12-13 36.979028910573845 39.0 38.0 39.0 34.5 39.0 14-15 38.180250509758224 40.0 38.0 41.0 34.0 41.0 16-17 38.12172116224876 40.0 38.0 41.0 34.0 41.0 18-19 38.095920113603256 40.0 38.0 41.0 34.0 41.0 20-21 38.03528437226915 40.0 38.0 41.0 34.0 41.0 22-23 37.885045878240604 40.0 38.0 41.0 34.0 41.0 24-25 37.788545004369354 40.0 38.0 41.0 34.0 41.0 26-27 37.67493992135159 40.0 37.5 41.0 34.0 41.0 28-29 37.51932165744247 40.0 37.0 41.0 33.5 41.0 30-31 37.21336840955432 40.0 36.5 41.0 33.0 41.0 32-33 36.821508156131664 40.0 35.0 41.0 33.0 41.0 34-35 36.53519334401398 39.5 35.0 41.0 32.5 41.0 36-37 36.268895645208275 39.0 35.0 41.0 31.5 41.0 38-39 35.9698186717157 39.0 35.0 41.0 31.0 41.0 40-41 35.690380133993585 39.0 35.0 41.0 30.0 41.0 42-43 35.303260632100205 39.0 35.0 41.0 28.0 41.0 44-45 34.85857304107195 39.0 35.0 41.0 24.0 41.0 46-47 34.41222873579959 39.0 35.0 41.0 17.5 41.0 48-49 34.0284190212642 39.0 35.0 41.0 5.0 41.0 50-51 33.32808767841538 38.5 34.0 40.5 2.0 40.5 52-53 33.33434496067579 38.5 35.0 40.0 2.0 41.0 54-55 33.40221926886105 39.0 35.0 41.0 2.0 41.0 56-57 33.184348601806 39.0 35.0 41.0 2.0 41.0 58-59 32.95424919895135 38.5 34.5 41.0 2.0 41.0 60-61 32.621395281095246 38.0 34.0 40.5 2.0 41.0 62-63 32.24992171570055 38.0 33.5 40.0 2.0 41.0 64-65 31.88483833381882 37.0 33.0 40.0 2.0 41.0 66-67 31.476787794931546 37.0 33.0 40.0 2.0 41.0 68-69 31.029549592193415 36.0 32.0 39.0 2.0 41.0 70-71 30.593824643169242 35.5 31.0 39.0 2.0 41.0 72-73 30.124146154966503 35.0 31.0 38.5 2.0 40.0 74-75 29.64272502184678 35.0 30.0 37.5 2.0 40.0 76-77 29.146535464608213 35.0 29.5 37.0 2.0 39.0 78-79 28.59378094960676 35.0 29.0 36.5 2.0 39.0 80-81 28.076787794931548 35.0 27.5 36.0 2.0 38.5 82-83 27.5850422371104 34.0 26.5 35.5 2.0 37.0 84-85 27.102503277017185 34.0 26.0 35.0 2.0 37.0 86-87 26.671240533061464 34.0 25.0 35.0 2.0 36.0 88-89 26.157748325080107 34.0 22.5 35.0 2.0 36.0 90-91 25.625438756189922 33.5 19.0 35.0 2.0 35.5 92-93 25.10060442761433 33.0 11.0 35.0 2.0 35.0 94-95 24.658613093504222 33.0 2.0 35.0 2.0 35.0 96-97 24.00586586076318 32.5 2.0 35.0 2.0 35.0 98-99 23.22434823769298 32.0 2.0 35.0 2.0 35.0 100-101 20.82512743955724 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38613281556185797 1101 2 0.3361577390838235 1101 3 0.038006255255311316 1101 4 0.15032850947645926 1101 5 0.2352383886222711 1101 6 0.19981595330278878 1101 7 0.12613610282053145 1101 8 0.16444350759559967 1101 9 0.1316131338053168 1101 10-11 0.20381483395327393 1101 12-13 -0.017823746716381095 1101 14-15 0.338981197326909 1101 16-17 0.3693143145267541 1101 18-19 -0.25093176292389785 1101 20-21 -0.1529116804114352 1101 22-23 -0.14348582749708783 1101 24-25 0.0985736536676356 1101 26-27 0.018047729514357513 1101 28-29 -0.05108437327664461 1101 30-31 0.3306149606520137 1101 32-33 0.3397656203312067 1101 34-35 0.35318604858885294 1101 36-37 0.4248126834752881 1101 38-39 0.4148823160771826 1101 40-41 -0.0735165488585352 1101 42-43 0.34388667377238846 1101 44-45 0.6250079305798479 1101 46-47 0.8282660739939658 1101 48-49 0.8035724059042693 1101 50-51 1.0499291179446217 1101 52-53 0.9691754075199981 1101 54-55 1.006333638228078 1101 56-57 0.7003219401060861 1101 58-59 0.723714838090082 1101 60-61 0.8284087844537567 1101 62-63 0.8809781092898845 1101 64-65 0.7923581215977471 1101 66-67 1.0220341067589835 1101 68-69 0.947372727546071 1101 70-71 1.15548797079542 1101 72-73 1.2365932312657186 1101 74-75 1.1391034852742088 1101 76-77 1.0261780161270657 1101 78-79 1.3291298003716143 1101 80-81 1.4030095393552635 1101 82-83 1.283800747758562 1101 84-85 1.5537570843185478 1101 86-87 1.3170642357982913 1101 88-89 1.18640078029431 1101 90-91 1.3810451479610784 1101 92-93 1.3784363298934075 1101 94-95 1.4086706642962668 1101 96-97 1.1143812012033543 1101 98-99 1.4205049437114532 1101 100-101 0.974516462203681 1102 1 0.5410867326125555 1102 2 0.5008402787663613 1102 3 0.3031752260694418 1102 4 0.23167771582566843 1102 5 0.44753997592385986 1102 6 0.5101078119817402 1102 7 0.5063844387140293 1102 8 0.33200623160993814 1102 9 0.4056146699036276 1102 10-11 0.36964944736853056 1102 12-13 0.2861637084807569 1102 14-15 0.06737981483842503 1102 16-17 0.4762011552845635 1102 18-19 0.3720201059957162 1102 20-21 0.48501714703351695 1102 22-23 0.3792356266759782 1102 24-25 0.4320669972416269 1102 26-27 0.2475664187104627 1102 28-29 0.23123769533575 1102 30-31 0.47657630217786817 1102 32-33 0.38674462698762824 1102 34-35 0.23701861387303325 1102 36-37 0.263682371135296 1102 38-39 0.22456613584164842 1102 40-41 0.7237184741829381 1102 42-43 0.6035192902598467 1102 44-45 0.894657188132328 1102 46-47 0.912111440097398 1102 48-49 0.866104407952399 1102 50-51 0.9298894354049381 1102 52-53 0.25319358468333064 1102 54-55 0.1664078829797333 1102 56-57 -0.038669355336828914 1102 58-59 -0.5587992428110979 1102 60-61 -0.4231913179527993 1102 62-63 -0.30143356505727326 1102 64-65 -0.22773916462857002 1102 66-67 -0.19455575499216593 1102 68-69 -0.12066362678060827 1102 70-71 -0.25487429238942383 1102 72-73 0.06170203822936671 1102 74-75 -0.14405063658958994 1102 76-77 -0.45852500486627434 1102 78-79 -0.4476879159622307 1102 80-81 -0.4859113515817519 1102 82-83 -0.48408839714671004 1102 84-85 -0.6871223319640904 1102 86-87 -0.7269385803819475 1102 88-89 -0.5530705458705647 1102 90-91 -0.8072229011940664 1102 92-93 -1.0520552215658867 1102 94-95 -1.1154268779464367 1102 96-97 -1.1006725622375022 1102 98-99 -1.0689023783571585 1102 100-101 -1.383138427709273 1103 1 0.4025420819711272 1103 2 0.34591749884358336 1103 3 0.4179933852424398 1103 4 0.3711564803044354 1103 5 -0.04522064183675667 1103 6 0.26867052215735754 1103 7 0.37699410367853403 1103 8 0.3981414912935861 1103 9 0.3606993628915447 1103 10-11 0.2637676439060854 1103 12-13 0.3072513283586886 1103 14-15 0.4943866027323125 1103 16-17 0.5487973690098116 1103 18-19 0.27180525981312087 1103 20-21 0.5112346087348527 1103 22-23 0.580506021494763 1103 24-25 0.7492064293004077 1103 26-27 0.2531399646065893 1103 28-29 0.1939821217898512 1103 30-31 0.47985348489054047 1103 32-33 0.5844031149687012 1103 34-35 0.5058565762593759 1103 36-37 0.8618032454658575 1103 38-39 1.001165027359896 1103 40-41 1.1166378912959019 1103 42-43 1.0444443743300909 1103 44-45 0.9931439237158415 1103 46-47 0.8272471979750904 1103 48-49 1.1756230279548916 1103 50-51 0.8336056516211485 1103 52-53 0.9266507399953241 1103 54-55 1.3635837454781807 1103 56-57 1.4724154621996064 1103 58-59 1.472213336588574 1103 60-61 0.8643911954361698 1103 62-63 0.8352895636013358 1103 64-65 0.999779829019456 1103 66-67 1.0316866164114948 1103 68-69 1.0546228347961737 1103 70-71 1.2379096782171253 1103 72-73 1.2661406358131266 1103 74-75 1.1208173419880687 1103 76-77 0.9725529625020144 1103 78-79 1.1409594872012967 1103 80-81 1.207707094052818 1103 82-83 1.3871362260940394 1103 84-85 1.0254430560045193 1103 86-87 0.928443472177527 1103 88-89 1.0635457824393093 1103 90-91 0.8943977863137178 1103 92-93 1.5003797018367777 1103 94-95 2.063432569058172 1103 96-97 1.6195292063513627 1103 98-99 1.8564230046295158 1103 100-101 2.247575235262456 1104 1 0.09353541296446011 1104 2 0.1294766424027216 1104 3 -0.15337902612996857 1104 4 0.013950443098394771 1104 5 -0.049278205894324856 1104 6 -0.10918837570154238 1104 7 0.14346655015098264 1104 8 0.21282734597944142 1104 9 0.08988153207371141 1104 10-11 0.12025062038905787 1104 12-13 0.03359039469776093 1104 14-15 -0.1112785429328369 1104 16-17 0.34187564208807686 1104 18-19 0.27059781860567966 1104 20-21 0.20231919981944202 1104 22-23 0.19278401377275145 1104 24-25 0.24107726117124173 1104 26-27 0.17697990844653333 1104 28-29 0.16312919093692102 1104 30-31 0.2501026951397449 1104 32-33 -0.03566006509448272 1104 34-35 -0.1257966353938329 1104 36-37 0.28671023037284016 1104 38-39 -0.10824178204691037 1104 40-41 0.11700293166094866 1104 42-43 -0.4346991848134749 1104 44-45 -0.7573369467650295 1104 46-47 -0.618782988055095 1104 48-49 -0.4497753574434924 1104 50-51 -0.6298832918677917 1104 52-53 -0.6317697584251718 1104 54-55 -0.750996809102368 1104 56-57 -0.7122177424336016 1104 58-59 -0.8467757824005417 1104 60-61 -0.5737485027035305 1104 62-63 -0.61495262664085 1104 64-65 -0.39615558691595965 1104 66-67 -0.6348490101241318 1104 68-69 -0.5316460314726861 1104 70-71 -0.4429608026533529 1104 72-73 -0.5665164368439513 1104 74-75 -0.467613686442963 1104 76-77 -0.24859831864926818 1104 78-79 -0.3840808778390645 1104 80-81 -0.2361679498222209 1104 82-83 -0.2816177226599059 1104 84-85 -0.18399183343036896 1104 86-87 -0.07748843375438241 1104 88-89 -0.2818315429380114 1104 90-91 -0.06906307714714899 1104 92-93 0.20254888400596016 1104 94-95 0.32499101061661406 1104 96-97 0.36589635271850796 1104 98-99 0.7037589119654193 1104 100-101 0.6145741822614035 1105 1 0.22654240597145048 1105 2 0.19227384519992796 1105 3 0.031096498345554835 1105 4 0.09269170183965514 1105 5 0.2333791367630198 1105 6 0.10158085506768799 1105 7 0.3933966200810488 1105 8 0.2773028704549674 1105 9 0.012399014591196078 1105 10-11 0.15983103996948245 1105 12-13 0.17603794714531062 1105 14-15 0.12669348503919053 1105 16-17 0.22572179593423414 1105 18-19 0.09458383259169523 1105 20-21 0.26840311590335375 1105 22-23 0.19166513265387408 1105 24-25 0.36604229613627837 1105 26-27 0.11656032802695648 1105 28-29 0.06907324688098271 1105 30-31 -0.1245826195455706 1105 32-33 -0.27747824691265777 1105 34-35 -0.1341183137155113 1105 36-37 -0.31587718221457095 1105 38-39 -0.06523478903991986 1105 40-41 -0.08950056484255242 1105 42-43 -0.19127261138689988 1105 44-45 0.21706864764056633 1105 46-47 0.32128694201483654 1105 48-49 -0.3915935392616774 1105 50-51 0.04920761722311795 1105 52-53 -0.00883269548810972 1105 54-55 -0.1612765293820928 1105 56-57 0.02652351630765537 1105 58-59 -0.1223002579250192 1105 60-61 -0.10472752368254845 1105 62-63 -0.29376381545203856 1105 64-65 -0.3759457967061728 1105 66-67 -0.39904481431993233 1105 68-69 -0.607100576927234 1105 70-71 -0.6485552082477604 1105 72-73 -0.7872856676131796 1105 74-75 -0.591180120009394 1105 76-77 -0.6774794375303834 1105 78-79 -1.0475074512656377 1105 80-81 -0.8623917260460026 1105 82-83 -1.0371421981843838 1105 84-85 -1.4321736516121888 1105 86-87 -1.0474185036844474 1105 88-89 -1.0380553191617885 1105 90-91 -0.8764057344898042 1105 92-93 -0.6513671999101227 1105 94-95 -0.6680859124603096 1105 96-97 -0.9386491018269432 1105 98-99 -0.4093180111115018 1105 100-101 -0.8491321114448915 1106 1 0.027191397267923634 1106 2 -0.09718130396745295 1106 3 -0.17225410788275042 1106 4 -0.38612803957001773 1106 5 -0.44904275788908876 1106 6 -0.21094115529604807 1106 7 0.039947910517234675 1106 8 -0.1003446618688244 1106 9 0.023014298587774817 1106 10-11 -0.7290103344834051 1106 12-13 -0.17529776749975667 1106 14-15 -0.6319848869485369 1106 16-17 -0.3628856397955005 1106 18-19 -0.1725087870189057 1106 20-21 -0.1010817158116879 1106 22-23 -0.44160447543587367 1106 24-25 -0.17333738892686057 1106 26-27 -0.046643374745094945 1106 28-29 -0.058649749547051044 1106 30-31 -0.10028317797994646 1106 32-33 0.18426145223710932 1106 34-35 -0.3877783227712044 1106 36-37 -0.666043203243909 1106 38-39 -0.23875191939158213 1106 40-41 -0.09021747383284406 1106 42-43 -0.2776161239893966 1106 44-45 -0.0311956779618896 1106 46-47 -0.03291640859139733 1106 48-49 0.11723576093452692 1106 50-51 0.20731749295890012 1106 52-53 0.09745849533545936 1106 54-55 0.022377945639284746 1106 56-57 -0.23041918128252092 1106 58-59 -0.17587977193618798 1106 60-61 -0.13983090950536337 1106 62-63 -0.19618872866831794 1106 64-65 -0.053336751075541144 1106 66-67 -0.22385489632426214 1106 68-69 -0.50523661355248 1106 70-71 -0.16644020095289846 1106 72-73 0.15898389016716763 1106 74-75 -0.27926182247958664 1106 76-77 -0.39525626501944444 1106 78-79 -0.12238042002350014 1106 80-81 0.09793930982460353 1106 82-83 0.12674068152583473 1106 84-85 0.45680607369847337 1106 86-87 0.6675409972462703 1106 88-89 0.004846024099265378 1106 90-91 0.017692972558542408 1106 92-93 -0.11993640049371024 1106 94-95 0.2183657653582749 1106 96-97 0.08800230432086309 1106 98-99 -0.05998209522882192 1106 100-101 -0.5451249675096896 1107 1 -0.009204486025438996 1107 2 -0.044298610122528004 1107 3 -0.20455326855421063 1107 4 -0.07040561750766727 1107 5 0.037817753701638424 1107 6 -0.1493903959035663 1107 7 -0.14023294479851245 1107 8 -0.04388982573772893 1107 9 -0.04050988206770256 1107 10-11 0.13387435776279943 1107 12-13 -0.1584487467163811 1107 14-15 0.02722524494595291 1107 16-17 -0.05801703467959385 1107 18-19 0.16251069739355728 1107 20-21 0.09584824022348215 1107 22-23 0.07625623599497544 1107 24-25 -0.06759698125299707 1107 26-27 0.15594455491117998 1107 28-29 0.27133626164398805 1107 30-31 0.2671228971599504 1107 32-33 0.2760255409661312 1107 34-35 0.35070588985868767 1107 36-37 0.03815593744354118 1107 38-39 -0.12752839820852557 1107 40-41 0.2999953559033628 1107 42-43 0.2831227848834956 1107 44-45 0.519601184548101 1107 46-47 0.3138811533590484 1107 48-49 0.428324390031257 1107 50-51 0.02363943540493807 1107 52-53 0.29754842339301035 1107 54-55 0.5209665747360148 1107 56-57 0.3563239242330738 1107 58-59 0.34896285396309423 1107 60-61 0.47275402254899035 1107 62-63 0.457863029924809 1107 64-65 0.5592232009628226 1107 66-67 0.42629997977486056 1107 68-69 0.20902947357781798 1107 70-71 0.21426773270017918 1107 72-73 0.0381805328530227 1107 74-75 -0.053604848059123356 1107 76-77 -0.21042912673007308 1107 78-79 -0.46129678692997445 1107 80-81 -0.36063133366060995 1107 82-83 -0.32061393478112166 1107 84-85 -0.35373299504653133 1107 86-87 0.02614161675067095 1107 88-89 0.13431744696097425 1107 90-91 0.06581697335790437 1107 92-93 0.2130097425918187 1107 94-95 0.21348217223277288 1107 96-97 0.03104786787002567 1107 98-99 -0.451518865812357 1107 100-101 -0.1303942520820307 1108 1 -0.12641544698640317 1108 2 0.004218657144740234 1108 3 0.013070605319661865 1108 4 -0.13656552741757366 1108 5 -0.3869194835355998 1108 6 -0.20381982533299237 1108 7 -0.26344991176547694 1108 8 -0.413634570482472 1108 9 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-0.879784548034312 2316 94-95 -1.2141383941805444 2316 96-97 -1.55228546546331 2316 98-99 -1.6564365277739945 2316 100-101 -1.5088608192192048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4120.0 3 567.0 4 417.0 5 388.0 6 379.0 7 400.0 8 454.0 9 522.0 10 627.0 11 861.0 12 1351.0 13 2012.0 14 2350.0 15 3174.0 16 4063.0 17 5451.0 18 4334.0 19 2897.0 20 2745.0 21 2564.0 22 2521.0 23 2452.0 24 2523.0 25 2634.0 26 2863.0 27 3206.0 28 3504.0 29 4201.0 30 4879.0 31 5906.0 32 7449.0 33 9624.0 34 13879.0 35 26916.0 36 29318.0 37 49220.0 38 55546.0 39 8323.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33585188708586 23.200819597153362 15.817859168328424 18.645469347432357 2 15.026431509419528 25.160028125603407 39.826874524105314 19.986665840871748 3 17.517926370711518 31.003512402716648 31.55879266621972 19.919768560352114 4 11.339090511587232 21.716586503425155 39.0088908322402 27.935432152747413 5 10.224779702771846 41.2653697859345 35.101420543582044 13.408429967711605 6 24.795144710942626 41.49376685864256 18.34912881825472 15.361959612160092 7 22.719305597562887 38.54305214941896 23.338058398895136 15.399583854123014 8 23.55755043154171 35.96132337028792 21.691118875259125 18.79000732291124 9 25.492303488478 18.92048456143547 22.983878313143276 32.603333636943255 10-11 22.12332461171806 30.890734437747348 27.64399775521657 19.341943195318024 12-13 24.04540707243587 30.38110809150677 28.43191399777995 17.141570838277403 14-15 21.032985810654488 31.779470354305534 28.663607398352802 18.523936436687176 16-17 16.827563339970812 34.519578140400036 29.981800063034818 18.671058456594334 18-19 16.42707105523482 32.016483546513 34.762101688616184 16.79434370963599 20-21 17.76417320323959 30.97360773274299 35.20912478249383 16.05309428152359 22-23 17.747539137864756 30.99256139174666 34.95526450510391 16.304634965284674 24-25 17.190136558947763 31.854825483714606 34.81487825589405 16.140159701443583 26-27 16.470326739275396 32.39226203272859 35.124016630289425 16.013394597706593 28-29 16.421225233432022 32.20387155545434 35.45306308358005 15.92184012753359 30-31 16.769789127321623 32.02298574140677 34.94430293953671 16.262922191734898 32-33 16.046773482394354 32.70408739601135 35.18924749206535 16.05989162952895 34-35 16.534664108604673 32.32869274650954 34.89258508198005 16.244058062905733 36-37 16.855478144266787 31.687169321809094 34.55013190689268 16.90722062703144 38-39 16.188280121595604 32.29569078725687 34.88096343935611 16.63506565179142 40-41 16.31208903298665 31.879929327334654 35.414109032622356 16.393872607056338 42-43 16.242632219332183 31.639927724712756 34.73439560519624 17.383044450758817 44-45 16.229789698140625 31.506310512543635 34.80369216425724 17.460207625058498 46-47 16.821580632354923 31.201627325075304 34.459373326874534 17.51741871569524 48-49 16.5662025284183 31.365935919055648 34.12472364774056 17.94313790478549 50-51 16.381134480560867 31.190749339888917 34.1788218155331 18.249294364017118 52-53 16.654738816502316 31.381718520946578 34.18631119852131 17.777231464029793 54-55 17.11806535614455 30.891658851487314 33.73608063444081 18.25419515792732 56-57 17.029354991441163 31.023782641949232 33.32738463779728 18.619477728812324 58-59 16.742237178506652 30.33837379542425 33.97741261135269 18.941976414716404 60-61 16.965033691495172 30.782371152795484 33.707339282462215 18.545255873247132 62-63 16.807148072682512 30.622397270126715 34.02435982177162 18.546094835419158 64-65 17.001028853420255 30.310385956606055 33.73473791552476 18.953847274448925 66-67 17.008321345982264 30.531327955716602 33.152460896957336 19.307889801343798 68-69 16.70703105083363 30.038385618867952 33.681741701932395 19.572841628366024 70-71 17.17558086162051 29.723940460317806 33.58360108704363 19.516877591018048 72-73 17.03783575062246 29.137881103252823 33.62505942287412 20.1992237232506 74-75 16.79958912118193 29.76540203654563 33.22964228409283 20.205366558179602 76-77 17.09561401112659 29.169876259936085 33.43713294545057 20.297376783486758 78-79 16.946711386421658 28.872829107170823 33.40489222466214 20.77556728174538 80-81 17.2990571500366 29.17991354477354 32.74846773224372 20.77256157294614 82-83 17.48603331609501 28.591094827774583 32.88216270549417 21.04070915063624 84-85 16.946387243503484 28.502364438552824 32.96029163533201 21.59095668261168 86-87 17.266505350207836 28.64241093456123 33.033823655256164 21.057260059974762 88-89 17.55480754519027 28.119057506939736 33.11694462051831 21.209190327351678 90-91 17.699308495522345 27.996640847584835 32.9572199916932 21.34683066519962 92-93 17.907631560734487 27.874994080814187 32.86769071616684 21.34968364228448 94-95 17.7012155911912 27.20115274405184 33.399155844510716 21.69847582024625 96-97 18.031410293774773 26.825709664257403 33.24930053625554 21.89357950571229 98-99 17.949932156159218 27.09437123785413 33.03767382138402 21.918022784602638 100-101 18.422454041435657 26.379486431281002 33.611759556463376 21.586299970819958 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5734.0 1 3258.5 2 936.0 3 1046.5 4 762.5 5 724.5 6 1424.0 7 2493.0 8 3327.0 9 3281.0 10 2295.0 11 1428.0 12 1223.5 13 1166.0 14 1059.0 15 955.0 16 896.0 17 920.5 18 1000.0 19 1057.5 20 1195.0 21 1321.0 22 1411.5 23 1545.5 24 1711.0 25 2017.0 26 2543.0 27 3114.0 28 3321.0 29 3652.5 30 4456.0 31 5260.0 32 6179.0 33 7657.5 34 9100.5 35 9894.5 36 11065.5 37 12426.5 38 12482.5 39 11908.5 40 11344.0 41 10386.0 42 9378.5 43 9504.5 44 9625.5 45 9080.0 46 8396.5 47 8046.0 48 8626.5 49 8141.5 50 6610.0 51 5366.0 52 4461.0 53 3662.0 54 2939.5 55 2502.5 56 2152.5 57 1757.5 58 1483.0 59 1262.0 60 1010.0 61 817.5 62 686.0 63 544.0 64 425.5 65 351.0 66 281.0 67 232.0 68 186.5 69 171.0 70 168.0 71 133.0 72 97.5 73 94.5 74 85.0 75 59.0 76 51.5 77 37.5 78 25.5 79 22.0 80 14.0 81 9.0 82 7.5 83 7.5 84 6.0 85 4.5 86 2.0 87 0.5 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48609088260996214 2 0.057165744247014275 3 0.066996795805418 4 0.07282260413632391 5 0.08665889892222546 6 0.10923390620448588 7 0.07901252548791145 8 0.05789397028837752 9 0.060078648412467225 10-11 0.08228954267404602 12-13 0.11706233614914069 14-15 0.063173609088261 16-17 0.06863530439848528 18-19 0.0671788523157588 20-21 0.08101514710166036 22-23 0.07045586950189339 24-25 0.09212059423244975 26-27 0.07318671715700553 28-29 0.07373288668802797 30-31 0.0760996213224585 32-33 0.07682784736382174 34-35 0.07773812991552578 36-37 0.07427905621905039 38-39 0.04496795805418002 40-41 0.049155257792018646 42-43 0.048791144771337024 44-45 0.021300611709874746 46-47 0.02876492863384795 48-49 0.029493154675211186 50-51 0.02366734634430527 52-53 0.026398193999417417 54-55 0.024577628896009323 56-57 0.025487911447713372 58-59 0.022575007282260414 60-61 0.03277017186134576 62-63 0.01765948150305855 64-65 0.022757063792601225 66-67 0.01638508593067288 68-69 0.021118555199533935 70-71 0.048791144771337024 72-73 0.04533207107486164 74-75 0.038778036702592486 76-77 0.026398193999417417 78-79 0.0149286338479464 80-81 0.017113311972036117 82-83 0.021118555199533935 84-85 0.01838770754442179 86-87 0.00892076900669968 88-89 0.048791144771337024 90-91 0.06044276143314885 92-93 0.039688319254296535 94-95 0.059896591902126414 96-97 0.05243227497815321 98-99 0.0409627148266822 100-101 0.17477424992717738 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 274640.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.315422174352314 #Duplication Level Percentage of deduplicated Percentage of total 1 74.8749526422492 30.186153260569128 2 12.682582787008373 10.226073586388328 3 4.334742908455892 5.242709711150374 4 2.0990422430145426 3.3849509675572285 5 1.185238260329546 2.3891690421190273 6 0.7777506991747661 1.881320867017703 7 0.505856497840582 1.427567278905768 8 0.3937597857955227 1.2699673599703227 9 0.2935814479971657 1.0652274016707055 >10 2.521222736843814 20.47038098686169 >50 0.24200562237997142 6.606869738838336 >100 0.07835427937712189 5.364907782242568 >500 0.004959131606146955 1.5450550548248703 >1k 0.003967305284917564 4.387652462886464 >5k 0.001983652642458782 4.551994498997495 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6045 2.2010632100203904 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5339 1.943999417419167 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4311 1.569691232158462 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3085 1.1232886688027963 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2266 0.82508010486455 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1311 0.4773521701136032 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 966 0.3517331779784445 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 914 0.3327993009030003 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 779 0.2836440431109817 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 660 0.2403145936498689 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 545 0.19844159627148264 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 496 0.1806000582580833 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 455 0.1656714244101369 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 443 0.16130206816195747 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 411 0.14965045150014564 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 395 0.14382464316923974 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 385 0.14018351296242354 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 334 0.12161374890766094 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 333 0.12124963588697932 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 299 0.10886979318380426 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 292 0.10632100203903291 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 288 0.10486454995630642 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 284 0.10340809787357996 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.6411302068161956E-4 0.0 18-19 0.0 0.0 0.0 3.6411302068161956E-4 0.0 20-21 0.0 0.0 0.0 0.0014564520827264785 0.0 22-23 0.0 0.0 0.0 0.005279638799883484 0.0 24-25 0.0 0.0 0.0 0.015110690358287212 0.0 26-27 0.0 0.0 0.0 0.0371395281095252 0.0 28-29 0.0 0.0 0.0 0.07427905621905039 0.0 30-31 0.0 0.0 0.0 0.16421497232741045 0.0 32-33 0.0 0.0 0.0 0.3160501019516458 0.0 34-35 0.0 0.0 0.0 0.568562481794349 0.0 36-37 0.0 0.0 0.0 0.9028182347800757 0.0 38-39 0.0 0.0 0.0 1.2856830760267988 0.0 40-41 0.0 0.0 0.0 1.6585348092047771 0.0 42-43 0.0 0.0 0.0 2.002257500728226 0.0 44-45 0.0 0.0 0.0 2.4233542091465194 0.0 46-47 0.0 0.0 0.0 2.8850495193708126 0.0 48-49 0.0 0.0 0.0 3.3254442178852317 0.0 50-51 0.0 0.0 0.0 3.7272429362073987 0.0 52-53 0.0 0.0 0.0 4.10501019516458 0.0 54-55 0.0 0.0 0.0 4.521737547334693 0.0 56-57 0.0 0.0 0.0 5.014564520827265 0.0 58-59 0.0 0.0 0.0 5.478626565685989 0.0 60-61 0.0 0.0 0.0 5.936862802213808 0.0 62-63 0.0 0.0 0.0 6.354864549956306 0.0 64-65 0.0 0.0 0.0 6.819290707835712 0.0 66-67 0.0 0.0 0.0 7.345069909699971 0.0 68-69 0.0 0.0 0.0 7.9010704922808035 0.0 70-71 0.0 0.0 0.0 8.470179143606176 0.0 72-73 0.0 0.0 0.0 9.007063792601222 0.0 74-75 0.0 0.0 0.0 9.554689775706379 0.0 76-77 0.0 0.0 0.0 10.142368191086515 0.0 78-79 0.0 0.0 0.0 10.762452665307311 0.0 80-81 0.0 0.0 0.0 11.422771628313429 0.0 82-83 0.0 0.0 0.0 12.040671424410135 0.0 84-85 0.0 0.0 0.0 12.687882318671715 0.0 86-87 0.0 0.0 0.0 13.331270026216139 0.0 88-89 0.0 3.6411302068161956E-4 0.0 14.02181036993883 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2040 0.0 41.90612 1 GCGGTAT 50 0.0015711308 38.20714 1 CGGTATC 50 0.0016125784 38.00525 2 CCGTATC 95 0.0 32.522266 94-95 CGTATCA 45 2.399423E-5 31.68836 94-95 GTATCAA 3015 0.0 31.36407 1 GTATAGT 140 1.6085323E-8 30.70217 1 AATGCAG 100 4.9349168E-5 28.503937 5 CATGGGG 995 0.0 28.169718 4 TCCCATC 105 6.837957E-5 27.171358 8 GTACATG 1865 0.0 27.144485 1 AAGTGCC 35 0.008397082 27.136717 64-65 CGCCGTT 45 8.9058804E-4 26.406967 94-95 ATGGGGG 315 0.0 25.63846 5 ACATGGG 1770 0.0 24.961075 3 TACATGG 1875 0.0 24.576727 2 TATCAAC 3870 0.0 23.937414 2 ATCAACG 3895 0.0 23.783772 3 GACAATG 100 0.0016403946 23.77494 7 ATGGGGA 705 0.0 23.584816 5 >>END_MODULE