##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938201_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 274640 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46226332653656 33.0 31.0 34.0 31.0 34.0 2 32.65824351878823 34.0 31.0 34.0 31.0 34.0 3 32.68718322167201 34.0 31.0 34.0 31.0 34.0 4 36.17577556073405 37.0 35.0 37.0 35.0 37.0 5 36.156958199825226 37.0 35.0 37.0 35.0 37.0 6 36.22229464025634 37.0 37.0 37.0 35.0 37.0 7 36.1775087387125 37.0 37.0 37.0 35.0 37.0 8 36.20084838333819 37.0 37.0 37.0 35.0 37.0 9 37.863592339062045 39.0 38.0 39.0 35.0 39.0 10-11 37.877929289251384 39.0 38.0 39.0 35.0 39.0 12-13 37.8475385959802 39.0 38.0 39.0 35.0 39.0 14-15 39.06785610253422 40.5 38.5 41.0 36.0 41.0 16-17 39.02714644625692 40.0 38.0 41.0 36.0 41.0 18-19 39.07904711622488 40.0 38.5 41.0 35.5 41.0 20-21 39.05742790562191 40.0 38.5 41.0 35.0 41.0 22-23 38.893799155257796 40.0 38.0 41.0 35.0 41.0 24-25 38.797048863967376 40.0 38.0 41.0 35.0 41.0 26-27 38.6329067142441 40.0 38.0 41.0 35.0 41.0 28-29 38.48970834547043 40.0 38.0 41.0 35.0 41.0 30-31 38.144751310806875 40.0 37.0 41.0 34.5 41.0 32-33 37.787618336731725 40.0 35.5 41.0 34.0 41.0 34-35 37.492674046023886 40.0 35.0 41.0 33.0 41.0 36-37 37.253779493154674 40.0 35.0 41.0 33.0 41.0 38-39 37.026188829012526 40.0 35.0 41.0 33.0 41.0 40-41 36.87629988348383 40.0 35.0 41.0 33.0 41.0 42-43 36.601795077191966 40.0 35.0 41.0 32.5 41.0 44-45 36.23950262161375 40.0 35.0 41.0 31.0 41.0 46-47 35.72403510049519 40.0 35.0 41.0 29.0 41.0 48-49 35.24114659190212 39.5 35.0 41.0 25.0 41.0 50-51 34.859536120011654 39.0 35.0 41.0 23.0 41.0 52-53 34.459734561607924 39.0 35.0 41.0 20.0 41.0 54-55 34.19562518205651 39.0 35.0 41.0 14.5 41.0 56-57 33.92295368482377 38.0 35.0 40.5 5.0 41.0 58-59 33.54002694436353 38.0 34.5 40.0 2.0 41.0 60-61 33.12310297116225 38.0 34.0 40.0 2.0 41.0 62-63 32.70675247596854 37.5 33.5 40.0 2.0 41.0 64-65 32.269270681619574 37.0 33.0 40.0 2.0 41.0 66-67 31.75665598601806 36.5 33.0 40.0 2.0 41.0 68-69 31.197085275269444 36.0 32.0 39.0 2.0 41.0 70-71 30.676396373434315 35.5 31.0 39.0 2.0 40.0 72-73 30.265851660355374 35.0 31.0 38.0 2.0 40.0 74-75 29.831062481794348 35.0 30.5 37.0 2.0 39.5 76-77 28.509974876201575 33.5 28.5 36.0 2.0 39.0 78-79 29.104005243227498 35.0 30.0 36.0 2.0 39.0 80-81 28.905154019807746 35.0 30.0 36.0 2.0 38.5 82-83 28.54049300903 35.0 29.0 35.5 2.0 37.0 84-85 28.160295295659772 34.5 29.0 35.0 2.0 37.0 86-87 27.787529129041655 34.0 29.0 35.0 2.0 36.0 88-89 27.39370084474221 34.0 27.0 35.0 2.0 36.0 90-91 27.095494101369066 34.0 26.5 35.0 2.0 36.0 92-93 26.787350713661517 34.0 26.0 35.0 2.0 35.0 94-95 26.49301085056802 34.0 25.0 35.0 2.0 35.0 96-97 26.071220506845325 34.0 24.0 35.0 2.0 35.0 98-99 25.596034809204777 33.5 19.0 35.0 2.0 35.0 100-101 23.65159299446548 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24792096794349305 1101 2 0.2495843072696573 1101 3 0.23365673706724266 1101 4 0.011427022884447524 1101 5 0.11681865861845608 1101 6 0.18066007005750606 1101 7 0.09365960247283311 1101 8 0.10468690149151172 1101 9 0.21210095028986586 1101 10-11 -0.08100991144055314 1101 12-13 0.048747582427779435 1101 14-15 0.09690949948151939 1101 16-17 0.214879875953649 1101 18-19 0.22591227138437375 1101 20-21 0.08539517595264101 1101 22-23 0.11243307862697804 1101 24-25 0.03678187935054211 1101 26-27 -0.10063128516392794 1101 28-29 -0.1642140825082663 1101 30-31 -0.01184311819342554 1101 32-33 0.043830519743515595 1101 34-35 -0.10433480778667814 1101 36-37 0.16865679393741573 1101 38-39 0.39224182902088245 1101 40-41 0.558651357447502 1101 42-43 0.3138203450556105 1101 44-45 0.6293694025087433 1101 46-47 0.5627515892002535 1101 48-49 0.41580655071773265 1101 50-51 0.5377442219242425 1101 52-53 0.6199760930679901 1101 54-55 0.7772696684232301 1101 56-57 0.5295043121632403 1101 58-59 0.383517168242939 1101 60-61 0.43788092049261707 1101 62-63 0.4016914722053926 1101 64-65 0.587943073404297 1101 66-67 0.4167972954386876 1101 68-69 0.7086012929911902 1101 70-71 0.5047170318732164 1101 72-73 0.4781272726573711 1101 74-75 0.2138037773699999 1101 76-77 0.6475600090364679 1101 78-79 0.40160632274620767 1101 80-81 0.6091348740905786 1101 82-83 0.756463047782173 1101 84-85 0.4459086773812828 1101 86-87 0.7213274915893066 1101 88-89 0.6681858918216577 1101 90-91 0.5138734991924991 1101 92-93 0.86680297908282 1101 94-95 0.638677308831916 1101 96-97 0.5916204351468899 1101 98-99 0.9247867011347388 1101 100-101 1.07868961068122 1102 1 0.06627734275147645 1102 2 0.07504513676274627 1102 3 0.05924557475285752 1102 4 -0.13853457465778973 1102 5 -0.023606328580719094 1102 6 0.03396268142463299 1102 7 -0.07121494949849705 1102 8 0.10135868848588103 1102 9 0.02412091956789908 1102 10-11 -0.12056444293057211 1102 12-13 0.12792064950406967 1102 14-15 0.061675244489201475 1102 16-17 0.16038038798641452 1102 18-19 0.21122333129221005 1102 20-21 -0.04078889982821465 1102 22-23 0.23192872634843553 1102 24-25 -0.06620071153527363 1102 26-27 -0.09547895541481921 1102 28-29 0.1721594453975186 1102 30-31 -0.12173815147555445 1102 32-33 -2.6830258111232297E-4 1102 34-35 -0.03703450056702451 1102 36-37 -0.09964837759356726 1102 38-39 0.13020905892359735 1102 40-41 -0.061676343525356 1102 42-43 0.0317542928282748 1102 44-45 0.36218430266234947 1102 46-47 0.1427567095279514 1102 48-49 0.4195187883009339 1102 50-51 0.5901955788110769 1102 52-53 0.7674415308560185 1102 54-55 0.3508423770765816 1102 56-57 0.618758024400826 1102 58-59 0.5165176802757045 1102 60-61 0.48844415653972817 1102 62-63 0.2935245495223384 1102 64-65 0.44556596126911785 1102 66-67 0.3724104546808782 1102 68-69 0.7669090246860186 1102 70-71 0.7528929151297419 1102 72-73 0.30387612058364155 1102 74-75 0.27201550292043564 1102 76-77 0.17796195476099186 1102 78-79 -0.09871369773509997 1102 80-81 -0.37707224316492827 1102 82-83 -0.2196314222639053 1102 84-85 0.0030643353433923437 1102 86-87 -0.507295140259135 1102 88-89 -0.46305450756390343 1102 90-91 -0.4609728909764712 1102 92-93 -0.5968746962884026 1102 94-95 -0.31725587089158935 1102 96-97 -0.22884807483774594 1102 98-99 -0.14772993993029004 1102 100-101 0.6183721503637578 1103 1 -0.2932630732405528 1103 2 -0.17705661937127104 1103 3 -0.07350757009706399 1103 4 0.07931634077377225 1103 5 0.10116014295994091 1103 6 0.11566650506394183 1103 7 0.2328702416834716 1103 8 0.06586236266697654 1103 9 -0.20746146927255182 1103 10-11 -0.218397298827945 1103 12-13 -0.04531076163056724 1103 14-15 0.08403948661150906 1103 16-17 0.049929211002982754 1103 18-19 0.10091227138437375 1103 20-21 -0.151037685480226 1103 22-23 0.03816237935627953 1103 24-25 -0.2143978718292061 1103 26-27 0.0036694441368041453 1103 28-29 -0.04446933776352324 1103 30-31 -0.16553252188283096 1103 32-33 -0.011135160222160323 1103 34-35 -0.007648836100706546 1103 36-37 -0.008681258400642378 1103 38-39 -0.33373414695508785 1103 40-41 -0.1624983637022197 1103 42-43 -0.12858634735108154 1103 44-45 -0.08893819079885645 1103 46-47 0.10967351112217472 1103 48-49 0.5833312182423924 1103 50-51 0.5608566200366383 1103 52-53 0.43078690387880414 1103 54-55 0.4377694539230177 1103 56-57 0.5710637287226561 1103 58-59 0.7446282793540533 1103 60-61 0.6002575828692756 1103 62-63 0.651959597473514 1103 64-65 0.8255556860169051 1103 66-67 0.9795922332336211 1103 68-69 1.23010493949484 1103 70-71 0.9976385247947093 1103 72-73 0.5852701298002323 1103 74-75 0.9463756349418588 1103 76-77 0.9532764397528979 1103 78-79 0.8447101408500295 1103 80-81 0.8109259508816571 1103 82-83 1.1078143991335274 1103 84-85 1.2405783470509526 1103 86-87 0.9721854924473057 1103 88-89 0.7211138197495863 1103 90-91 0.6613960217150208 1103 92-93 0.7364404737203145 1103 94-95 0.7501637953184037 1103 96-97 0.22949044801690732 1103 98-99 0.2417643931124296 1103 100-101 0.20862311561472424 1104 1 0.048007548030071234 1104 2 -0.07898712130177188 1104 3 -0.12466938125887594 1104 4 -0.04913574767832074 1104 5 -0.03137758957778658 1104 6 -0.04592001652257949 1104 7 -0.11866362985040269 1104 8 -0.02169116488655476 1104 9 0.1095901277790432 1104 10-11 0.023189222758581707 1104 12-13 0.014562878243076227 1104 14-15 0.29188785445987264 1104 16-17 0.05975722083099555 1104 18-19 -0.06610071562861464 1104 20-21 -0.031192125634660783 1104 22-23 -0.043115261921364834 1104 24-25 -0.0737520311833677 1104 26-27 -0.06280300233564162 1104 28-29 -0.2084637217579015 1104 30-31 -0.36562378197409373 1104 32-33 -0.11795158703858277 1104 34-35 -0.2051356735875416 1104 36-37 -0.2414225711419533 1104 38-39 -0.5208173340382771 1104 40-41 -0.41012209132594535 1104 42-43 -0.018084416849148965 1104 44-45 -0.4451544070150675 1104 46-47 -0.4398385983899402 1104 48-49 -0.35071581580464084 1104 50-51 -0.2351489959689701 1104 52-53 -0.17015377706186996 1104 54-55 -0.16060190444834177 1104 56-57 0.08304688070580823 1104 58-59 -0.09615094142517222 1104 60-61 -0.24067607806438218 1104 62-63 -0.3869593214454099 1104 64-65 -0.029221428760202173 1104 66-67 -0.2821276684862788 1104 68-69 -0.463296253906357 1104 70-71 -0.3244604573042764 1104 72-73 -0.23966493513483655 1104 74-75 -0.14805047948425454 1104 76-77 -0.37417880770235 1104 78-79 -0.2926433165034297 1104 80-81 -0.24329658334087867 1104 82-83 -0.1334070820879525 1104 84-85 0.08634879282139707 1104 86-87 -0.04134206108024685 1104 88-89 -0.14490934627357888 1104 90-91 -0.46883212851312805 1104 92-93 -0.40881751153767 1104 94-95 -0.20982918467457878 1104 96-97 -0.48251376398730983 1104 98-99 -0.4114542801062413 1104 100-101 -0.33117330418169644 1105 1 0.0937418137643391 1105 2 0.1671667248520734 1105 3 0.09085509426559923 1105 4 0.16568942714685164 1105 5 0.12037066217046544 1105 6 0.0661079555053945 1105 7 -0.023279014465785508 1105 8 -0.10854431173969914 1105 9 0.14133837952729777 1105 10-11 0.03570670527606268 1105 12-13 -0.04704551336531182 1105 14-15 0.13370603627805622 1105 16-17 0.03702994810372218 1105 18-19 0.04047271094481175 1105 20-21 -0.05797534241787616 1105 22-23 -0.4352830940891934 1105 24-25 -0.18144433887567857 1105 26-27 -0.17706873660137745 1105 28-29 -0.14524693854112058 1105 30-31 0.05689370054339804 1105 32-33 0.2089015598145565 1105 34-35 0.3733957949439244 1105 36-37 0.41284316312378166 1105 38-39 0.1435183302973826 1105 40-41 -0.35865355985740877 1105 42-43 -0.1840984028631354 1105 44-45 -0.23522433708500046 1105 46-47 0.008902660351324698 1105 48-49 -0.14652001160883543 1105 50-51 0.11443142361144254 1105 52-53 -0.04386007076816867 1105 54-55 7.267668803336846E-4 1105 56-57 0.13738254504146852 1105 58-59 0.11300989773567238 1105 60-61 -0.2110257284140289 1105 62-63 -0.3456306501167319 1105 64-65 -0.29796268750146737 1105 66-67 -0.3521975985562058 1105 68-69 -0.342666883276987 1105 70-71 -0.10334157618539308 1105 72-73 -0.269874725344625 1105 74-75 -0.3026658640996409 1105 76-77 -0.28893405245759496 1105 78-79 -0.030475484335596548 1105 80-81 -0.12189798194227919 1105 82-83 -0.08844204712292125 1105 84-85 0.04648865296125493 1105 86-87 0.15816842843024048 1105 88-89 -0.27183242319665624 1105 90-91 -0.35072024040124106 1105 92-93 -0.1386776513978134 1105 94-95 -0.10570331054870152 1105 96-97 -0.07167460314814633 1105 98-99 -0.13599973465169413 1105 100-101 -0.13075372376211547 1106 1 -0.2671526874179335 1106 2 -0.2149583443233567 1106 3 -0.1273116310953668 1106 4 -0.19095392949650147 1106 5 -0.23798321416902724 1106 6 -0.16654787786986702 1106 7 -0.31721170048480474 1106 8 -0.22853223748302298 1106 9 -0.268029231279165 1106 10-11 -0.20236996625384762 1106 12-13 -0.2664573964462633 1106 14-15 -0.48205589962776685 1106 16-17 -0.37107992763206 1106 18-19 -0.20869718390853365 1106 20-21 -0.3268036364260354 1106 22-23 -0.23604528154202598 1106 24-25 -0.14484424831874776 1106 26-27 -0.16468004615513365 1106 28-29 -0.3485095033144745 1106 30-31 -0.08144457988122156 1106 32-33 -0.20495616519424686 1106 34-35 -0.18342867554961373 1106 36-37 -0.31422832653763066 1106 38-39 -0.1494962091200307 1106 40-41 -0.15189449158755508 1106 42-43 0.055512705453004685 1106 44-45 0.4052772476611892 1106 46-47 -0.07112702219635025 1106 48-49 -0.0913305967071949 1106 50-51 -0.31316076836923656 1106 52-53 -0.3933846469114499 1106 54-55 -0.7842382680847066 1106 56-57 -0.4574632566388601 1106 58-59 -0.3031620598031921 1106 60-61 -0.2204210093920409 1106 62-63 -0.2489737099346172 1106 64-65 -0.6386328087471256 1106 66-67 -0.6657509516779037 1106 68-69 -0.4253629641745036 1106 70-71 -0.5809287372257899 1106 72-73 -0.08970417646903783 1106 74-75 -0.2658268038792855 1106 76-77 -0.3155980359561106 1106 78-79 -0.0722574433837444 1106 80-81 -0.23703105031275484 1106 82-83 -0.29672297482830956 1106 84-85 -0.460152188211957 1106 86-87 -0.05550818272838498 1106 88-89 -0.004261864259191839 1106 90-91 0.19985328679099013 1106 92-93 0.5374807226022895 1106 94-95 0.21588696967467058 1106 96-97 -0.4395118019205988 1106 98-99 -0.5315196823299182 1106 100-101 -0.693678209348473 1107 1 -0.02142427015174775 1107 2 0.08490176758711243 1107 3 0.11312102278152736 1107 4 -0.09720392949650147 1107 5 -0.037943246143449016 1107 6 0.07450927640671523 1107 7 -0.03828484197160975 1107 8 -0.11604722549261481 1107 9 -0.03244270050378617 1107 10-11 0.055398818718174425 1107 12-13 0.020225757030949865 1107 14-15 0.19462775344977246 1107 16-17 -0.14052687007809794 1107 18-19 0.17482100154310132 1107 20-21 0.09804398547645121 1107 22-23 0.23495291989681988 1107 24-25 0.24114695871561764 1107 26-27 0.2361742703916292 1107 28-29 0.081321631777449 1107 30-31 0.1679683897430806 1107 32-33 0.4619852816482819 1107 34-35 0.184851700149828 1107 36-37 0.19767465108026983 1107 38-39 0.5288985750526294 1107 40-41 0.4564688177649643 1107 42-43 0.6664989164841799 1107 44-45 0.654915037429376 1107 46-47 0.413198017771677 1107 48-49 0.21863393167011225 1107 50-51 0.426385094940116 1107 52-53 0.459757839099737 1107 54-55 0.5629839541375148 1107 56-57 0.5686908200997465 1107 58-59 0.31109653332230636 1107 60-61 0.6293491744608701 1107 62-63 0.6879017896657089 1107 64-65 0.38060180356302453 1107 66-67 0.4708647557561427 1107 68-69 0.1299802612451586 1107 70-71 0.18204838107956434 1107 72-73 0.41190703456213384 1107 74-75 0.6133573487985728 1107 76-77 0.5942365963380531 1107 78-79 0.4631142592541444 1107 80-81 0.4553650328207368 1107 82-83 0.5208479684170975 1107 84-85 0.8137162170638241 1107 86-87 0.7257917773035913 1107 88-89 0.7512712092819775 1107 90-91 0.8571274674464675 1107 92-93 0.9724577409875828 1107 94-95 1.1252844516890583 1107 96-97 1.2089319430833996 1107 98-99 0.7923462249442643 1107 100-101 1.2451082614748685 1108 1 -0.0036877836652635665 1108 2 0.023715581400928443 1108 3 -0.11839173998123442 1108 4 0.015127151584579224 1108 5 -0.03831862151881893 1108 6 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-1.2569440706669077 2316 86-87 -1.5185644478140325 2316 88-89 -1.2771569032768362 2316 90-91 -1.5126698543052512 2316 92-93 -1.730458020865477 2316 94-95 -1.5674069163891176 2316 96-97 -1.990064844828293 2316 98-99 -2.0787743867097355 2316 100-101 -1.8038591199945486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 5.0 5 19.0 6 29.0 7 84.0 8 151.0 9 280.0 10 479.0 11 797.0 12 1206.0 13 1766.0 14 2271.0 15 3119.0 16 3715.0 17 5030.0 18 4764.0 19 3228.0 20 2716.0 21 2655.0 22 2770.0 23 3002.0 24 3182.0 25 3413.0 26 3183.0 27 2823.0 28 3209.0 29 3825.0 30 4526.0 31 5357.0 32 6512.0 33 8448.0 34 12355.0 35 24529.0 36 26863.0 37 51214.0 38 67419.0 39 9691.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.83309059131955 23.811535100495192 16.073769297990097 19.281605010195165 2 14.42623547834954 25.028952256090896 40.34414945919371 20.200662806365855 3 17.086821473958654 30.89567076079397 32.33510911401532 19.68239865123206 4 10.811243810078649 22.35362656568599 38.24898048354209 28.586149140693273 5 9.636615205359744 41.916326827847364 35.4180017477425 13.029056219050394 6 24.4196038450335 41.73208563938247 17.92273521701136 15.925575298572678 7 22.262598310515582 38.5439120302942 22.85246140401981 16.341028255170407 8 23.326706428098078 36.43232181999578 21.665610731217093 18.57536102068905 9 24.823131456202503 18.779561533503983 22.49025083837328 33.90705617192023 10-11 22.04504078065832 30.706925429653364 27.595943780949604 19.652090008738714 12-13 23.73689193125546 29.993992135158752 28.317251674919895 17.95186425866589 14-15 20.7191232158462 31.671460821438973 27.822968249344598 19.78644771337023 16-17 16.990454759785102 34.975486046253366 29.230136233134708 18.80392296082683 18-19 16.260195164579088 32.08127002621614 34.1332289542674 17.525305854937372 20-21 17.427177395863676 30.96417127876493 34.62860471890475 16.980046606466647 22-23 17.47305563646956 31.4926813282843 34.30581852607049 16.728444509175645 24-25 17.20725132953786 32.4157807632576 34.237352321616164 16.139615585588373 26-27 16.327675179996284 32.80411815476949 34.80219528094716 16.066011384287062 28-29 15.781779387160258 32.23807937314188 35.294085519380864 16.686055720316997 30-31 16.73754733469269 31.936353043984855 35.129624235362655 16.1964753859598 32-33 15.526381904973437 33.10473020947499 34.983742294430144 16.385145591121436 34-35 16.069724757278017 32.74689177476565 35.081429310181576 16.10195415777475 36-37 16.773235067072548 31.937384389064484 34.33608372052376 16.95329682333921 38-39 15.88609427321278 32.04258627690548 34.965430970281744 17.105888479599994 40-41 15.990378715183384 31.744413227263706 35.61623382416938 16.64897423338353 42-43 16.252758377091087 31.374673182384257 35.06871363129875 17.303854809225907 44-45 15.79131346674823 31.623408779748903 34.865019371376974 17.720258382125902 46-47 16.864076609379552 30.94268861054471 34.1332289542674 18.060005825808332 48-49 16.559314011069034 30.98237692979901 34.12631080687446 18.3319982522575 50-51 15.962532770171862 31.040088843577045 34.43908389163997 18.558294494611125 52-53 16.271482668220216 31.281859889309644 34.66101077774541 17.78564666472473 54-55 16.576791436061754 30.96945091756481 33.64167637634722 18.812081270026216 56-57 17.004078065831635 31.321548208563936 32.78400815613166 18.890365569472763 58-59 16.03699388290125 31.31808913486746 33.852133702301195 18.792783279930088 60-61 16.581889018351294 32.46540926303524 32.422079813574136 18.530621905039325 62-63 15.855301485581125 32.745958345470434 32.773812991552575 18.624927177395865 64-65 16.177177395863676 32.11531459364987 32.431728808622196 19.27577920186426 66-67 16.424228080396155 32.44756772502185 31.586622487620158 19.541581706961843 68-69 16.162671725428655 31.498803884371846 32.28128561493451 20.057238775264985 70-71 16.687117681328285 31.08924410136906 32.127330323332366 20.096307893970287 72-73 16.548208563938246 30.46934168365861 32.10748616370521 20.87496358869793 74-75 16.381080687445383 31.124016894844157 31.464280512671134 21.03062190503932 76-77 16.314448004660647 30.461877366734637 32.227279347509466 20.99639528109525 78-79 16.21012962423536 29.917710457325953 32.09128313428488 21.7808767841538 80-81 16.56295514127585 30.264710166035535 31.44607486163705 21.726259831051557 82-83 16.70404893678998 29.76350859306729 31.493955723856686 22.038486746286047 84-85 16.249981793553463 29.592758203824808 31.62660034665074 22.530659655970986 86-87 16.33601989524411 30.07043804856202 31.501956207887822 22.091585848306046 88-89 16.5560369938829 29.626602097291 31.246540926303524 22.570819982522575 90-91 16.56935728473144 29.15603909852734 31.529514144906322 22.745089471834895 92-93 16.74522419389782 29.17005747534495 31.27572690745505 22.80899142330218 94-95 16.34703612001165 28.737984270317508 31.73973201281678 23.175247596854064 96-97 16.391851863988975 28.71218451825029 31.64512023944116 23.25084337831958 98-99 16.312081270026216 29.02690795222837 31.2638362947859 23.39717448295951 100-101 16.211768132828432 28.528983396446257 31.87882318671716 23.380425284008155 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5413.0 1 3083.0 2 895.0 3 1006.0 4 735.5 5 697.0 6 1454.0 7 2554.0 8 3219.5 9 3139.5 10 2262.0 11 1385.0 12 1188.0 13 1161.0 14 1027.5 15 910.5 16 871.0 17 862.0 18 903.5 19 960.5 20 1010.5 21 1100.0 22 1212.5 23 1331.5 24 1482.5 25 1788.0 26 2321.0 27 2888.0 28 3175.0 29 3575.0 30 4387.5 31 5141.0 32 6127.0 33 7623.5 34 9194.5 35 10129.0 36 11115.0 37 12415.5 38 12688.5 39 12239.5 40 11642.5 41 10693.5 42 9675.5 43 10051.0 44 10490.5 45 9923.5 46 9040.0 47 8623.5 48 9174.5 49 8560.5 50 7200.0 51 5875.0 52 4435.5 53 3414.5 54 2756.0 55 2368.0 56 1968.5 57 1554.0 58 1256.0 59 1003.5 60 803.5 61 598.5 62 425.0 63 330.0 64 235.0 65 160.0 66 108.0 67 70.0 68 50.0 69 37.0 70 29.0 71 21.0 72 19.0 73 22.0 74 16.0 75 12.5 76 10.5 77 7.0 78 4.5 79 3.0 80 2.5 81 1.0 82 1.0 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.018205651034080977 3 0.006918147392950771 4 0.0 5 0.0 6 0.0 7 0.0 8 7.282260413632391E-4 9 3.6411302068161956E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.8205651034080978E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005279638799883484 26-27 0.018569764054762596 28-29 1.8205651034080978E-4 30-31 0.0 32-33 3.6411302068161956E-4 34-35 0.0167491989513545 36-37 0.004369356248179435 38-39 0.016203029420332072 40-41 0.014746577337605593 42-43 0.00801048645499563 44-45 0.0029129041654529565 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.6411302068161956E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.004369356248179435 86-87 5.461695310224294E-4 88-89 0.0 90-91 1.8205651034080978E-4 92-93 1.8205651034080978E-4 94-95 0.0 96-97 1.8205651034080978E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 274640.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.79372938515803 #Duplication Level Percentage of deduplicated Percentage of total 1 74.3633053064099 28.84829942242752 2 12.753934373072301 9.895453573300639 3 4.497083755743072 5.233759507280604 4 2.122205408676704 3.293130492956911 5 1.229141051642196 2.3841482666797953 6 0.8136773705673376 1.8939347832369744 7 0.571652746683345 1.5523579369981186 8 0.4093177025332639 1.270316814770404 9 0.35653439960592975 1.244816911333101 >10 2.5066954160918873 19.519514577116354 >50 0.26889462069574616 7.101096082500338 >100 0.09162335223706906 5.853574904568152 >500 0.0079672480206147 2.1805542050942277 >1k 0.005975436015461026 5.482679788180756 >5k 0.001991812005153675 4.246362733556105 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5880 2.140984561607923 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5111 1.8609816487037578 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4421 1.6097436644334402 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3082 1.1221963297407516 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2129 0.7751966210311682 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1973 0.7183949898048354 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1431 0.5210457325953977 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1155 0.42055053888727056 TruSeq Adapter, Index 11 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 966 0.3517331779784445 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 905 0.3295222837168657 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 788 0.2869210602971162 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 702 0.255607340518497 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 699 0.2545150014564521 RNA PCR Primer, Index 11 (95% over 23bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 568 0.20681619574715993 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 514 0.18715409263035246 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 502 0.18278473638217302 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 494 0.17987183221672007 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 450 0.1638508593067288 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 409 0.1489222254587824 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 368 0.133993591610836 TruSeq Adapter, Index 11 (96% over 28bp) TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 335 0.12197786192834256 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 333 0.12124963588697932 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 326 0.11870084474220799 TruSeq Adapter, Index 11 (96% over 26bp) GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 324 0.11797261870084474 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 313 0.11396737547334693 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 312 0.1136032624526653 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 300 0.10923390620448588 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 293 0.10668511505971454 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 287 0.10450043693562483 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.6411302068161956E-4 0.0 18-19 0.0 0.0 0.0 3.6411302068161956E-4 0.0 20-21 0.0 0.0 0.0 0.0014564520827264785 0.0 22-23 0.0 0.0 0.0 0.005279638799883484 0.0 24-25 0.0 0.0 0.0 0.01547480337896883 0.0 26-27 0.0 0.0 0.0 0.0371395281095252 0.0 28-29 0.0 0.0 0.0 0.07373288668802797 0.0 30-31 0.0 0.0 0.0 0.16494319836877366 0.0 32-33 0.0 0.0 0.0 0.3191450626274396 0.0 34-35 0.0 0.0 0.0 0.5740241771045733 0.0 36-37 0.0 0.0 0.0 0.9075517040489367 0.0 38-39 0.0 0.0 0.0 1.2895062627439557 0.0 40-41 0.0 0.0 0.0 1.6670914651907953 0.0 42-43 0.0 0.0 0.0 2.0151835129624236 0.0 44-45 0.0 0.0 0.0 2.4373725604427614 0.0 46-47 0.0 0.0 0.0 2.9032551704048934 0.0 48-49 0.0 0.0 0.0 3.3503859598019226 0.0 50-51 0.0 0.0 0.0 3.7581925429653364 0.0 52-53 0.0 0.0 0.0 4.1401471016603555 0.0 54-55 0.0 0.0 0.0 4.558877075444218 0.0 56-57 0.0 0.0 0.0 5.053342557529858 0.0 58-59 0.0 0.0 0.0 5.520863676085057 0.0 60-61 0.0 0.0 0.0 5.985471890474804 0.0 62-63 0.0 0.0 0.0 6.408389163996505 0.0 64-65 0.0 0.0 0.0 6.875364113020682 0.0 66-67 0.0 0.0 0.0 7.403874162540053 0.0 68-69 0.0 0.0 0.0 7.96315176230702 0.0 70-71 0.0 0.0 0.0 8.53644771337023 0.0 72-73 0.0 0.0 0.0 9.079340227206526 0.0 74-75 0.0 0.0 0.0 9.629879114477134 0.0 76-77 0.0 0.0 0.0 10.221016603553743 0.0 78-79 0.0 0.0 0.0 10.848565394698515 0.0 80-81 0.0 0.0 0.0 11.51780512671133 0.0 82-83 0.0 0.0 0.0 12.143169239732014 0.0 84-85 0.0 0.0 0.0 12.795113603262454 0.0 86-87 0.0 0.0 0.0 13.444145062627438 0.0 88-89 0.0 0.0 0.0 14.145244683949898 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCGTC 20 0.0020694616 71.275406 2 GCGTGCG 25 0.005017371 56.99956 9 CGTCAGA 25 0.005017371 56.99956 5 GTGTAGA 50 2.8153909E-5 47.516937 1 CATTCCG 45 9.6126256E-4 42.221897 9 TTTTCGA 50 0.0016137357 37.999706 7 GGTATCA 2440 0.0 35.248222 1 TCCGGTG 35 2.052034E-4 33.92831 12-13 GTATCAA 3595 0.0 31.854193 1 TAGACAG 60 0.0039459113 31.666424 5 TAGGCAT 60 0.0039459113 31.666424 5 ATGGGGA 620 0.0 29.878803 5 CATGGGG 1035 0.0 29.371754 4 GATCAGT 65 0.005826268 29.241192 1 TGGGGAA 565 0.0 28.58385 6 ATCAACG 4155 0.0 27.550932 3 AACGCAG 4140 0.0 27.53602 6 CAACGCA 4185 0.0 27.353436 5 TCAACGC 4195 0.0 27.288229 4 GGACTCT 70 0.008380962 27.142649 6 >>END_MODULE