FastQCFastQC Report
Thu 26 May 2016
SRR938200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275226
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60792.2087302798427473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52331.9013465297609964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43151.5678024605233518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29721.0798398407127234No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21390.7771794815896754No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13120.47669914906295185No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.3469875665816456No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9180.3335440692376447No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8230.29902698146250717No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7040.25578978730207175No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.19002565164628343No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4960.18021553196282328No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4820.17512880323806618No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA4620.16786204791698459No Hit
GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4060.14751513301795616No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT3770.13697833780238786No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.13661500003633378No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3300.11990146279784614No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3210.11663142290335943No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT3060.11118135641254824No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770.10064456119697994No Hit
GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2760.10028122343092585No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTATC405.3460384E-447.5627827
CGCAGAA554.9202245E-543.2310189
GTCTTCG459.5328386E-442.293451
GGTATCA21650.041.3166921
TTCGATA500.001606108738.0363664
CCGAGAA250.001614717238.00174784-85
CTTCGAT550.002564164634.578513
TCTTCGA550.002564164634.578512
CATTCCG600.003923824531.7027459
CGTATCT300.00394476631.6738994-95
GTATCAA31100.029.83321
AGTTTAC404.4655328E-429.7159114-15
GTCATGA650.00580403829.2640749
CTAGTCC650.00580403829.2640748
GGTACTC856.358996E-427.9679163
TAAAGTG856.358996E-427.9679165
TACTCCC856.358996E-427.9679165
TGACGCG350.00837849927.14904894-95
TGGGGAA5450.026.1670446
ATGGGGA5400.025.5336695
Produced by FastQC (version 0.11.5)