##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938200_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 275226 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47476982552521 33.0 31.0 34.0 31.0 34.0 2 32.66389076613402 34.0 31.0 34.0 31.0 34.0 3 32.68497889007579 34.0 31.0 34.0 31.0 34.0 4 36.17896928342526 37.0 35.0 37.0 35.0 37.0 5 36.17153902610945 37.0 35.0 37.0 35.0 37.0 6 36.23107918583273 37.0 37.0 37.0 35.0 37.0 7 36.187802024518035 37.0 37.0 37.0 35.0 37.0 8 36.201808695399414 37.0 37.0 37.0 35.0 37.0 9 37.84674413027839 39.0 38.0 39.0 35.0 39.0 10-11 37.86023304484314 39.0 38.0 39.0 35.0 39.0 12-13 37.79891616344386 39.0 38.0 39.0 35.0 39.0 14-15 38.99392135917392 40.0 38.0 41.0 35.5 41.0 16-17 38.897958405092545 40.0 38.0 41.0 35.5 41.0 18-19 39.11861888048368 40.5 38.5 41.0 35.5 41.0 20-21 39.12932826113812 41.0 39.0 41.0 35.0 41.0 22-23 38.99442276529107 40.0 38.5 41.0 35.0 41.0 24-25 38.86726726399395 40.0 38.0 41.0 35.0 41.0 26-27 38.62539149644292 40.0 38.0 41.0 35.0 41.0 28-29 38.43834521447828 40.0 37.5 41.0 34.5 41.0 30-31 38.07817938712186 40.0 37.0 41.0 34.0 41.0 32-33 37.68074963847892 40.0 35.0 41.0 33.5 41.0 34-35 37.39700646014548 40.0 35.0 41.0 33.0 41.0 36-37 37.24952221083764 40.0 35.0 41.0 33.0 41.0 38-39 36.99278047858851 40.0 35.0 41.0 33.0 41.0 40-41 36.777272132720014 40.0 35.0 41.0 33.0 41.0 42-43 36.45653026966929 40.0 35.0 41.0 31.5 41.0 44-45 36.051256058657245 39.5 35.0 41.0 30.5 41.0 46-47 35.485591840887125 39.0 35.0 41.0 28.0 41.0 48-49 34.9819893469367 39.0 35.0 41.0 24.0 41.0 50-51 34.56578775261058 39.0 35.0 41.0 22.5 41.0 52-53 34.115396074498776 38.0 35.0 40.0 19.0 41.0 54-55 33.783959364304245 38.0 34.5 40.0 11.5 41.0 56-57 33.405187009948186 38.0 34.0 40.0 4.5 41.0 58-59 32.94278338529064 38.0 33.0 40.0 2.0 41.0 60-61 32.42728702956843 37.0 33.0 40.0 2.0 41.0 62-63 31.94618786015856 37.0 32.5 40.0 2.0 41.0 64-65 31.42929265403705 36.0 31.5 39.0 2.0 41.0 66-67 30.843899195570184 35.5 31.0 39.0 2.0 40.0 68-69 30.28405019874576 35.0 30.0 39.0 2.0 40.0 70-71 29.80389934090529 35.0 29.5 38.0 2.0 40.0 72-73 29.308811667502344 35.0 29.0 37.5 2.0 39.5 74-75 28.7996446556648 35.0 28.0 37.0 2.0 39.0 76-77 27.44772477890897 32.5 26.5 35.0 2.0 37.5 78-79 28.091223212923197 34.0 28.0 36.0 2.0 38.0 80-81 27.823083574952946 34.0 27.0 35.5 2.0 37.0 82-83 27.358618008473037 34.0 26.0 35.0 2.0 37.0 84-85 26.87478472237361 34.0 25.5 35.0 2.0 36.0 86-87 26.388349937869243 33.0 24.5 35.0 2.0 36.0 88-89 25.823381511921113 33.0 21.5 35.0 2.0 35.5 90-91 25.433500105367955 33.0 19.5 35.0 2.0 35.0 92-93 24.82168290786481 32.0 14.5 35.0 2.0 35.0 94-95 24.220507873529392 32.0 4.5 34.0 2.0 35.0 96-97 23.444053250782993 31.0 2.0 34.0 2.0 35.0 98-99 22.362571123367708 31.0 2.0 34.0 2.0 35.0 100-101 18.414045184684586 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16594248252847166 1101 2 0.25152378279960175 1101 3 0.11027665072857928 1101 4 0.2251820829606146 1101 5 0.18625547779078033 1101 6 0.13484012853622573 1101 7 0.21579684840934021 1101 8 0.23497150063828087 1101 9 -0.013785121669435796 1101 10-11 0.14119220613994088 1101 12-13 0.3672673434672902 1101 14-15 0.22955990843406937 1101 16-17 0.49748052098575357 1101 18-19 0.40783551582976685 1101 20-21 0.369711860493922 1101 22-23 0.5800398578680586 1101 24-25 0.610773854164897 1101 26-27 0.6602832234868856 1101 28-29 0.46472609934216536 1101 30-31 0.6439749682537794 1101 32-33 0.6312067585080428 1101 34-35 0.5893037342834972 1101 36-37 0.9446706627043469 1101 38-39 0.878583042277711 1101 40-41 0.6064775567196818 1101 42-43 0.9846816846430428 1101 44-45 0.8956331733603733 1101 46-47 0.8038382826768853 1101 48-49 0.634985203141774 1101 50-51 0.4844504816476416 1101 52-53 0.02910276884080787 1101 54-55 0.10355410660880437 1101 56-57 0.43183465068148763 1101 58-59 0.2814946578250357 1101 60-61 0.2057158080745083 1101 62-63 0.6919100743920836 1101 64-65 0.6472184430348307 1101 66-67 0.17367335088067648 1101 68-69 0.8150148191627196 1101 70-71 0.6650226654487632 1101 72-73 0.4082569818522259 1101 74-75 0.27314022146653727 1101 76-77 0.4068581998903049 1101 78-79 0.6119681335297429 1101 80-81 0.8643692679525152 1101 82-83 0.9457374840228638 1101 84-85 0.7432167614754626 1101 86-87 0.9652442619499304 1101 88-89 0.5149184247899434 1101 90-91 0.5498412259872723 1101 92-93 -0.07380293994028975 1101 94-95 0.22676922724732762 1101 96-97 -0.0065946962337832815 1101 98-99 0.44828238391685815 1101 100-101 -1.00416542644329 1102 1 0.21389093031200446 1102 2 0.27733043144742453 1102 3 0.26775828830550097 1102 4 0.19111425270422444 1102 5 0.32183963953280426 1102 6 0.19753959560602397 1102 7 0.2541861470487632 1102 8 0.2667793403621914 1102 9 0.2594294094588818 1102 10-11 0.432150716989419 1102 12-13 0.3411552906934574 1102 14-15 0.3920425385744011 1102 16-17 0.48411145536967837 1102 18-19 0.3063879002724903 1102 20-21 0.38891414491678233 1102 22-23 0.3266918651366808 1102 24-25 0.24974800170563327 1102 26-27 0.4497757945315186 1102 28-29 0.38299995522196184 1102 30-31 0.5052222387043983 1102 32-33 0.2534829354591892 1102 34-35 0.5368811160539266 1102 36-37 0.6779230639442844 1102 38-39 0.5441349838844403 1102 40-41 0.3164810688671338 1102 42-43 0.2163001738088255 1102 44-45 0.009999383447869548 1102 46-47 0.2743438792074997 1102 48-49 0.47461749657404795 1102 50-51 0.5930445537535576 1102 52-53 0.5708973234852479 1102 54-55 0.7512246366376587 1102 56-57 0.8878335512266347 1102 58-59 0.8877523883669767 1102 60-61 0.7020738638718385 1102 62-63 0.5671066782982024 1102 64-65 0.6746590037568012 1102 66-67 0.44048966556908553 1102 68-69 0.2924683595600506 1102 70-71 -0.07332998469553687 1102 72-73 -0.21501175850845655 1102 74-75 0.15577341584099358 1102 76-77 0.7220657983449605 1102 78-79 0.5247370800382072 1102 80-81 -0.12314733839683711 1102 82-83 0.36245377886000796 1102 84-85 -0.48221729413862846 1102 86-87 -0.2865385346356497 1102 88-89 0.2760160472188211 1102 90-91 0.13955368800263557 1102 92-93 -0.19213940366591586 1102 94-95 -0.021623583539547298 1102 96-97 -0.12423636557273454 1102 98-99 -0.414453731648063 1102 100-101 -1.1787115195610092 1103 1 0.05822271456774075 1103 2 0.1744153490646596 1103 3 0.16444889526226802 1103 4 0.12170149393785579 1103 5 0.0713960400397724 1103 6 0.27830286838450746 1103 7 0.17559158290286803 1103 8 0.001816659049701741 1103 9 0.011337591405087721 1103 10-11 0.2348334377329806 1103 12-13 0.1865221404329347 1103 14-15 0.20122434395392474 1103 16-17 0.04924312696523003 1103 18-19 0.13831298124699032 1103 20-21 0.198694457548811 1103 22-23 0.08024066108091432 1103 24-25 0.04346015492348698 1103 26-27 0.32030107266135843 1103 28-29 0.3730928998776406 1103 30-31 0.3921677393381131 1103 32-33 0.3422732466829643 1103 34-35 0.45900476061103035 1103 36-37 0.1521450045160364 1103 38-39 0.041769119921617914 1103 40-41 -0.0024247190500616966 1103 42-43 0.09162055122046553 1103 44-45 0.09329046921043727 1103 46-47 0.4412992376077156 1103 48-49 0.3617812763233843 1103 50-51 0.25330367218757743 1103 52-53 0.19572481257129226 1103 54-55 -0.10753469303421781 1103 56-57 -0.30190028907754396 1103 58-59 -0.26417691736461535 1103 60-61 0.05502415256357551 1103 62-63 0.43733622343269474 1103 64-65 0.46138622527490014 1103 66-67 0.21166978104577794 1103 68-69 0.45892378837288916 1103 70-71 0.06676295996014403 1103 72-73 -0.05611160360069434 1103 74-75 -0.3076049815678239 1103 76-77 -0.45295884607131853 1103 78-79 -0.5330787205532559 1103 80-81 -0.844242958732 1103 82-83 -0.701875188891016 1103 84-85 -0.30098225681993895 1103 86-87 -0.3498606019501125 1103 88-89 -0.934144493550086 1103 90-91 -1.285745565623614 1103 92-93 -1.2013352915690305 1103 94-95 -0.9426417499949764 1103 96-97 -0.5025563205086598 1103 98-99 -0.6085672367881862 1103 100-101 -1.1101225884245487 1104 1 0.08044493678995934 1104 2 0.1966375712868782 1104 3 0.18296741378078707 1104 4 0.14022001245637483 1104 5 0.24176641041014335 1104 6 0.27830286838450746 1104 7 0.2644804717917566 1104 8 0.20552036275340413 1104 9 0.29281907288657294 1104 10-11 0.23112973402927395 1104 12-13 0.1791147330255214 1104 14-15 0.30122434395392617 1104 16-17 0.12146534918745289 1104 18-19 0.3049796479136617 1104 20-21 0.30054630940065863 1104 22-23 0.3876480684883177 1104 24-25 0.354571266034597 1104 26-27 0.47215292451321034 1104 28-29 0.3805003072850539 1104 30-31 0.18846403563441783 1104 32-33 0.1441250985348148 1104 34-35 0.45159735320362415 1104 36-37 0.009552411923444026 1104 38-39 0.12695430510679984 1104 40-41 0.13090861428327116 1104 42-43 0.08050944010934558 1104 44-45 0.4303275062474796 1104 46-47 0.4412992376077156 1104 48-49 -0.09562613108402473 1104 50-51 -0.020770401886494483 1104 52-53 0.10868777553424991 1104 54-55 0.2850578995583746 1104 56-57 0.24995156277430652 1104 58-59 0.11730456411687129 1104 60-61 -0.13386473632531448 1104 62-63 0.12807696417343095 1104 64-65 0.3373121512008268 1104 66-67 -0.3179598485838504 1104 68-69 -0.11329843384933014 1104 70-71 -0.19805185485467192 1104 72-73 -0.5283338258229193 1104 74-75 -0.792790166753008 1104 76-77 -1.1714773645898369 1104 78-79 -0.8034490909236283 1104 80-81 -0.5127614772505211 1104 82-83 -0.5685418555576796 1104 84-85 -0.5102415160792013 1104 86-87 -0.4739346760241858 1104 88-89 -1.0971074565130472 1104 90-91 -1.3209307508087988 1104 92-93 -1.3976315878653267 1104 94-95 -1.0907898981431252 1104 96-97 -1.6766303945827339 1104 98-99 -1.16227094049189 1104 100-101 -1.891604069906034 1105 1 0.20904067033269058 1105 2 0.02152960112590563 1105 3 0.2122960356889152 1105 4 0.11396690422446909 1105 5 0.10508273026356818 1105 6 0.1688276688033028 1105 7 0.2040040943542536 1105 8 0.0578957062948291 1105 9 0.2530206171870546 1105 10-11 0.23073057161597887 1105 12-13 0.18364947978641766 1105 14-15 0.31207370922025035 1105 16-17 0.2868429175685563 1105 18-19 0.30583032190921955 1105 20-21 0.26337839944122976 1105 22-23 0.44767293434357214 1105 24-25 0.5344757288012474 1105 26-27 0.31112688080165185 1105 28-29 0.38602968825612294 1105 30-31 0.5143114378069029 1105 32-33 0.7585145763518568 1105 34-35 0.7415594000561327 1105 36-37 0.9097748958915091 1105 38-39 0.871608146227075 1105 40-41 1.3583330351705953 1105 42-43 1.5546293197062582 1105 44-45 1.3089755889591643 1105 46-47 0.5290429884256795 1105 48-49 1.1554273959412384 1105 50-51 1.368864462660035 1105 52-53 1.2435719529979323 1105 54-55 1.70362222359973 1105 56-57 1.5352021844206902 1105 58-59 1.323684619083501 1105 60-61 1.1707419905429006 1105 62-63 1.2916092243488038 1105 64-65 1.4572571845734217 1105 66-67 1.3700063862021707 1105 68-69 1.5930030973638658 1105 70-71 1.5286396776672504 1105 72-73 1.1292895219064363 1105 74-75 0.5912891422379616 1105 76-77 0.7520339559179483 1105 78-79 0.792932272772223 1105 80-81 1.0790655087460763 1105 82-83 0.8844747653034624 1105 84-85 0.3333653416619349 1105 86-87 0.538328116803978 1105 88-89 0.39445778363876727 1105 90-91 0.2974961566640424 1105 92-93 0.3320111290565464 1105 94-95 0.2128549127455024 1105 96-97 0.16580515050299027 1105 98-99 -0.33328913182809927 1105 100-101 -1.2653444180280076 1106 1 0.10195431227423057 1106 2 0.07762315938543907 1106 3 0.21602180708622853 1106 4 -0.008052109037507194 1106 5 0.0097502938752001 1106 6 0.013232358544122746 1106 7 0.22088834409921532 1106 8 0.06651524885289462 1106 9 0.14384960132295532 1106 10-11 0.023373651587000666 1106 12-13 -0.11997871188382447 1106 14-15 0.06717816388263742 1106 16-17 0.08659009736658874 1106 18-19 0.41973247605561426 1106 20-21 0.08444527112389011 1106 22-23 0.14139826219667384 1106 24-25 0.2711139593555316 1106 26-27 -0.24568374060378773 1106 28-29 0.042711681839520566 1106 30-31 0.19147038926838178 1106 32-33 0.16599864572216916 1106 34-35 -0.15938978456175334 1106 36-37 0.020446569679521076 1106 38-39 0.3754743732921426 1106 40-41 0.3511550113079096 1106 42-43 0.2108751614792581 1106 44-45 0.018232356704629638 1106 46-47 -0.46915326510419675 1106 48-49 -0.6065667788447584 1106 50-51 -0.2859028983997476 1106 52-53 -0.3112796814624943 1106 54-55 -0.4936884199829237 1106 56-57 -0.5249128413788 1106 58-59 -0.5375303963666269 1106 60-61 -0.6730713069126324 1106 62-63 -0.8280442197720177 1106 64-65 -0.9574344782738393 1106 66-67 -1.0321625450041196 1106 68-69 -0.9111366486331498 1106 70-71 -1.4429069610068481 1106 72-73 -1.5477511510979873 1106 74-75 -1.3961142020854105 1106 76-77 -1.650223684131138 1106 78-79 -1.3747570097210868 1106 80-81 -1.2108941287304518 1106 82-83 -1.325321647902328 1106 84-85 -1.7364928720376689 1106 86-87 -1.8305749983548871 1106 88-89 -1.8454183196774672 1106 90-91 -1.8769202130744134 1106 92-93 -1.6427733823795023 1106 94-95 -1.5857612293069252 1106 96-97 -1.371090335695392 1106 98-99 -1.377480920346219 1106 100-101 -1.552265777638869 1107 1 0.24096421895873021 1107 2 0.2871301256969616 1107 3 0.18331105639246914 1107 4 0.08126621329638795 1107 5 0.07620703660334982 1107 6 0.08147201691448203 1107 7 -0.003140757685137885 1107 8 0.14251921727803563 1107 9 0.25276561736919945 1107 10-11 -0.03555602389359791 1107 12-13 0.016399956393222226 1107 14-15 0.09450422176988837 1107 16-17 0.055734154074812636 1107 18-19 0.22384180904386852 1107 20-21 0.017995717571722025 1107 22-23 0.05120286039363009 1107 24-25 0.13534637103651193 1107 26-27 -0.10978674711718384 1107 28-29 -0.04380286186347604 1107 30-31 -0.19614841186463394 1107 32-33 0.022876530379022597 1107 34-35 0.3526664635510812 1107 36-37 0.34513851348892644 1107 38-39 0.24325823790558587 1107 40-41 0.5298204126796051 1107 42-43 0.3085544954739916 1107 44-45 0.18166389418180273 1107 46-47 0.24276926433547885 1107 48-49 0.10567207433789605 1107 50-51 0.13455605859460462 1107 52-53 0.015274258924854678 1107 54-55 -0.3122120508043551 1107 56-57 -0.46753602790916204 1107 58-59 -0.1056622170973327 1107 60-61 -0.02924465232378992 1107 62-63 -0.057127312267951424 1107 64-65 0.06354353722602468 1107 66-67 0.005350575242037081 1107 68-69 0.29855723625376385 1107 70-71 -0.0808888155266807 1107 72-73 -0.14074314235594798 1107 74-75 0.0064652742550102005 1107 76-77 0.23915646511615662 1107 78-79 0.3135994008671332 1107 80-81 0.3294874727303885 1107 82-83 0.38831190541979055 1107 84-85 0.34504676188948835 1107 86-87 0.44580568289142875 1107 88-89 0.19878792340294993 1107 90-91 -0.1072614113662631 1107 92-93 -0.023519331278841094 1107 94-95 0.05672823855026721 1107 96-97 -0.00941008148613065 1107 98-99 0.7963964898250246 1107 100-101 -0.12518558957002668 1108 1 0.02587199650273675 1108 2 0.07985752593540951 1108 3 0.06528033895085628 1108 4 0.23485342137549736 1108 5 0.18228039378126937 1108 6 0.13922501502094065 1108 7 0.18866792455064285 1108 8 0.039987482934812135 1108 9 0.12894908044514608 1108 10-11 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3 2.0 4 6.0 5 14.0 6 35.0 7 75.0 8 145.0 9 288.0 10 448.0 11 803.0 12 1291.0 13 1900.0 14 2254.0 15 2998.0 16 3995.0 17 5142.0 18 4893.0 19 3485.0 20 3126.0 21 3163.0 22 3344.0 23 3421.0 24 3684.0 25 3843.0 26 3526.0 27 3451.0 28 3935.0 29 4772.0 30 5864.0 31 7082.0 32 8920.0 33 11596.0 34 17276.0 35 29439.0 36 33877.0 37 53987.0 38 40531.0 39 2614.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.692870353884224 23.84494355076071 16.028168297583964 19.4340177977711 2 14.442223837209303 24.871002906976745 40.29033430232558 20.39643895348837 3 17.04675111372737 30.899121372664446 32.505214351639886 19.5489131619683 4 10.896136266195782 22.314388902211274 38.129028507481124 28.66044632411182 5 9.807284248466704 41.83610440949917 35.18552161148737 13.171089730546754 6 24.353519535209887 41.599659909672596 18.16976175509863 15.877058800018895 7 22.11818650854207 38.63770137995684 22.833598569902552 16.41051354159854 8 23.41966238654778 36.20842507611926 21.668737691933174 18.70317484539978 9 24.580526549090564 18.820169606069197 22.695893556568056 33.903410288272184 10-11 22.058962452675257 30.681875985553692 27.456163298525578 19.802998263245478 12-13 23.718818637148946 29.75171178437966 28.37828754344181 18.151182035029585 14-15 20.807082179735925 31.70957685683765 27.79279573877468 19.69054522465174 16-17 16.91734445653576 34.86404537452697 29.26453516362335 18.95407500531392 18-19 16.33929933945194 31.80295466271355 34.125591332214256 17.732154665620254 20-21 17.44584450596964 30.96291774759652 34.494197495876115 17.097040250557725 22-23 17.462013036559046 31.447246989746606 34.26402302108086 16.826716952613488 24-25 17.239756775071445 32.411701974976246 34.15413571398725 16.19440553596506 26-27 16.38453906843381 32.69403389165283 34.820711484858506 16.100715555054855 28-29 15.894465660643364 32.096152378737614 35.24739379889466 16.76198816172436 30-31 16.700306482643853 31.79008028137541 35.210804466979994 16.298808769000743 32-33 15.61952722489881 33.14258100615494 34.93292784838642 16.304963920559832 34-35 16.128123836084338 32.612349088398545 35.12250070402703 16.137026371490084 36-37 16.822720237186893 31.786967801007172 34.37011764876865 17.020194313037283 38-39 15.931771070832296 32.02068886583667 34.943799607950716 17.103740455380322 40-41 15.950549101181771 31.691994658866918 35.61409405117678 16.743362188774537 42-43 16.2376485848671 31.231935005077727 35.07193297035297 17.458483439702206 44-45 15.922005915138831 31.481945746404772 34.85771693081322 17.738331407643173 46-47 17.032184459317072 30.835931198360623 34.07036399177403 18.061520350548278 48-49 16.65746207648288 30.808792805886092 34.09464983195567 18.439095285675357 50-51 16.05711846085369 30.881575481210316 34.32158202149209 18.739724036443903 52-53 16.315448105708942 31.301177107538464 34.43625469416824 17.947120092584353 54-55 16.685712832270806 30.93000397880153 33.49278819513206 18.891494993795614 56-57 16.968701510598173 31.438938329657073 32.60068534716141 18.99167481258335 58-59 16.221849118178284 31.298947192452637 33.51812885899211 18.961074830376965 60-61 16.757473634489088 32.55770436109622 32.114600270695 18.570221733719695 62-63 16.05757053226032 32.58120726590848 32.51361403904054 18.847608162790657 64-65 16.27679213879218 32.067891588594854 32.23832920261362 19.416987069999344 66-67 16.557077111196303 32.246401168632254 31.591589190878416 19.604932529293027 68-69 16.234864826064754 31.4757101242797 32.16225597002313 20.127169079632413 70-71 16.64598327279731 30.99456538526531 31.99372775323833 20.36572358869905 72-73 16.628484327678954 30.408644893346825 31.953621629674306 21.00924914929991 74-75 16.42655328736384 30.950005905172024 31.257256547927287 21.36618425953685 76-77 16.408678856507418 30.34201933682082 32.11761991027512 21.13168189639665 78-79 16.24552082046213 29.833334544740914 32.03258444116557 21.88856019363139 80-81 16.4691710635329 30.159595342955566 31.352381575313593 22.01885201819794 82-83 16.69840877536037 29.608663414917824 31.44704035124703 22.24588745847478 84-85 16.359615730553493 29.653688699592802 31.38642628324518 22.600269286608533 86-87 16.358676986092764 29.82560072979834 31.531613388601258 22.284108895507636 88-89 16.61890857156355 29.54666453466293 31.063900149543105 22.770526744230416 90-91 16.524384260622117 29.00780281802753 31.38989740402752 23.077915517322836 92-93 16.754764842377252 29.053500335251563 31.163132139995238 23.028602682375944 94-95 16.437855889182423 28.720052617999865 31.514112016744733 23.327979476072983 96-97 16.550625206676138 28.610881975660362 31.306302214114663 23.532190603548834 98-99 16.396620639534884 28.928960755813954 30.920421511627904 23.753997093023255 100-101 16.41878903936367 28.318186938369134 31.43261519678201 23.83040882548519 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5216.0 1 3003.0 2 901.0 3 959.5 4 703.5 5 709.0 6 1437.5 7 2465.5 8 3119.0 9 3093.0 10 2287.5 11 1426.0 12 1178.0 13 1081.5 14 980.5 15 891.0 16 832.0 17 824.5 18 854.0 19 942.5 20 981.0 21 1052.0 22 1202.5 23 1320.5 24 1518.5 25 1825.5 26 2294.5 27 2832.5 28 3047.5 29 3434.5 30 4268.5 31 5073.0 32 6043.0 33 7695.0 34 9300.0 35 10187.5 36 11259.5 37 12521.5 38 12762.5 39 12303.5 40 11699.5 41 10585.0 42 9669.5 43 10117.0 44 10521.5 45 10056.5 46 9091.0 47 8590.5 48 9166.0 49 8654.5 50 7326.0 51 6014.0 52 4566.0 53 3503.5 54 2802.0 55 2409.0 56 2052.0 57 1585.0 58 1299.5 59 1073.0 60 807.5 61 626.0 62 466.5 63 357.5 64 277.5 65 185.0 66 133.5 67 95.0 68 57.5 69 36.0 70 28.5 71 28.0 72 27.5 73 24.5 74 19.0 75 14.0 76 11.0 77 7.5 78 4.5 79 3.5 80 4.5 81 4.5 82 3.0 83 1.5 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008356768619243822 2 0.009446781917406059 3 0.008720106385297901 4 0.0 5 7.266755321081584E-4 6 0.001816688830270396 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.450066490811188E-4 14-15 0.0 16-17 0.0019983577132974355 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002725033245405594 26-27 0.019983577132974356 28-29 0.006176742022919346 30-31 0.0023616954793515147 32-33 0.0 34-35 0.008538437502270861 36-37 0.0 38-39 0.0019983577132974355 40-41 0.0012716821811892772 42-43 0.0016350199472433564 44-45 0.0 46-47 0.0 48-49 3.633377660540792E-4 50-51 0.003088371011459673 52-53 0.006358410905946386 54-55 0.006358410905946386 56-57 0.01344349734400093 58-59 0.020710252665082512 60-61 0.003088371011459673 62-63 0.018348557185730998 64-65 0.018893563834812117 66-67 0.011808477396757574 68-69 0.029248690167353375 70-71 0.016531868355460604 72-73 0.004178384309621911 74-75 0.015805192823352444 76-77 0.018711894951785078 78-79 0.02325361702746107 80-81 0.010900132981622375 82-83 0.03342707447697529 84-85 0.019801908249947316 86-87 0.029612027933407458 88-89 0.019801908249947316 90-91 0.025070305857731463 92-93 0.02161859708021771 94-95 0.012716821811892771 96-97 0.01344349734400093 98-99 0.009446781917406059 100-101 0.008356768619243822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 275226.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.79210721168286 #Duplication Level Percentage of deduplicated Percentage of total 1 74.30220000601419 28.82338908697205 2 12.905385795653892 10.012542187862698 3 4.419633645895652 5.143407066838348 4 2.1278345865945263 3.30172749647607 5 1.2069394824532673 2.340986290067008 6 0.8164569008025311 1.9003250177790063 7 0.5137570930010861 1.3950804162722457 8 0.43410282548400225 1.3471810677655895 9 0.31462472524621354 1.098446046587764 >10 2.5817144651349415 19.797516728214635 >50 0.27181181880072725 7.170024187097821 >100 0.09060393960024242 6.030637667385552 >500 0.006969533815403262 1.9863948714848145 >1k 0.0059738861274885105 5.470600225493081 >5k 0.0019912953758295035 4.181741643703303 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5801 2.1077223808797134 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5026 1.826135612187802 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4389 1.5946894552113535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3107 1.128890439130024 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2073 0.7531991890301062 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1995 0.724858843277888 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1379 0.5010427793885752 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1221 0.4436354123520307 TruSeq Adapter, Index 11 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 945 0.34335418892110486 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 865 0.3142871676367785 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 756 0.27468335113688386 RNA PCR Primer, Index 11 (95% over 23bp) GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 741 0.2692332846460727 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 732 0.26596324475158595 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 563 0.20455916228844662 TruSeq Adapter, Index 11 (96% over 28bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.19656573143525682 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 487 0.17694549206833657 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 482 0.17512880323806618 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 471 0.1711320878114713 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 446 0.1620486436601193 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 408 0.1482418085500643 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 375 0.13625166227027968 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 361 0.13116493354552258 TruSeq Adapter, Index 11 (96% over 26bp) ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 333 0.12099147609600837 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 321 0.11663142290335943 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 311 0.11299804524281863 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 296 0.10754797875200744 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 293 0.1064579654538452 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 288 0.10464127662357481 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 277 0.10064456119697994 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.816688830270396E-4 0.0 20-21 0.0 0.0 0.0 0.001816688830270396 0.0 22-23 0.0 0.0 0.0 0.005631735373838227 0.0 24-25 0.0 0.0 0.0 0.015260186174271325 0.0 26-27 0.0 0.0 0.0 0.03869547208475943 0.0 28-29 0.0 0.0 0.0 0.0793893018828163 0.0 30-31 0.0 0.0 0.0 0.1725854388756876 0.0 32-33 0.0 0.0 0.0 0.3311823737582932 0.0 34-35 0.0 0.0 0.0 0.5742553392484722 0.0 36-37 0.0 0.0 0.0 0.8927208911948725 0.0 38-39 0.0 0.0 0.0 1.298387506994252 0.0 40-41 0.0 0.0 0.0 1.6628152863464933 0.0 42-43 0.0 0.0 0.0 2.0168879393661934 0.0 44-45 0.0 0.0 0.0 2.4191028463880593 0.0 46-47 0.0 0.0 0.0 2.894166975503768 0.0 48-49 0.0 0.0 0.0 3.34016408333515 0.0 50-51 0.0 0.0 0.0 3.725302115352474 0.0 52-53 0.0 0.0 0.0 4.098268332206986 0.0 54-55 0.0 0.0 0.0 4.520466816361826 0.0 56-57 0.0 0.0 0.0 5.01787621808986 0.0 58-59 0.0 0.0 0.0 5.516375633116057 0.0 60-61 0.0 0.0 0.0 5.998706517552847 0.0 62-63 0.0 0.0 0.0 6.442523598787905 0.0 64-65 0.0 0.0 0.0 6.925217820990749 0.0 66-67 0.0 0.0 0.0 7.429167302507757 0.0 68-69 0.0 0.0 0.0 7.970540573928336 0.0 70-71 0.0 0.0 0.0 8.558602748286862 0.0 72-73 0.0 0.0 0.0 9.103427728484954 0.0 74-75 0.0 0.0 0.0 9.65660947730229 0.0 76-77 0.0 0.0 0.0 10.271921984114872 0.0 78-79 0.0 0.0 0.0 10.914484823381512 0.0 80-81 0.0 0.0 0.0 11.6037365655861 0.0 82-83 0.0 0.0 0.0 12.217959059100522 0.0 84-85 0.0 0.0 0.0 12.872875382413 0.0 86-87 0.0 0.0 0.0 13.546321931794235 0.0 88-89 0.0 0.0 0.0 14.251923873471256 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGA 20 0.00207076 71.264175 1 CGCAGAA 45 9.620083E-4 42.215275 9 GGTATCA 2280 0.0 40.007957 1 CTAGCAC 60 0.0039454433 31.667213 3 GCTTATG 60 0.0039489553 31.66146 9 GTTCTGG 80 4.479634E-4 29.693407 1 GTATCAA 3265 0.0 28.811245 1 GTGCATA 85 6.387268E-4 27.946735 1 TTAGAGA 70 0.008387406 27.138393 4 AGGTGAT 105 6.894799E-5 27.138391 7 CTTAGAG 90 8.929294E-4 26.389343 3 CATGGGC 265 1.8189894E-12 25.090214 4 GTACATG 1890 0.0 23.880411 1 TATCAAC 4015 0.0 23.31099 2 TTCTGGA 225 2.7830538E-9 23.226843 2 ATCAACG 4075 0.0 22.963587 3 TCAACGC 4085 0.0 22.90321 4 ACTATAC 125 2.2495906E-4 22.800392 3 ATACTTA 125 2.2523614E-4 22.796251 6 CAACGCA 4110 0.0 22.763897 5 >>END_MODULE