FastQCFastQC Report
Thu 26 May 2016
SRR938199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455480
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64730.44473300904169066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56770.390043147277874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.22947756066727126No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.10182207931404073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37200.039.4899751
GTATCAA59050.036.831891
ATCAACG71000.030.4583343
TCAACGC72300.029.976414
TATCAAC72400.029.8037132
CAACGCA73100.029.5183145
AACGCAG75950.028.5318816
ACCGTAC856.403655E-427.9444228
GTACATG50200.025.9380911
ACGCAGA83900.025.6495827
GTACCTG11000.025.5822321
CGCAGAG85800.025.0262158
TACATGG50400.024.801522
GCAGAGT89850.023.8956939
GAGTACT49550.023.53461512-13
ACATGGG52050.023.4674263
TACCTGG11800.022.9586282
TATAACG1252.2471932E-422.8136272
GTACTTT52700.021.85749414-15
AGAGTAC76000.021.25027710-11
Produced by FastQC (version 0.11.5)