FastQCFastQC Report
Thu 26 May 2016
SRR938199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455480
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62520.429549014758018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54790.376439387693407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35090.24108885041360925No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15350.10546348970786269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37350.044.1889151
GTATCAA57450.034.358371
ATCAACG72200.026.9383493
TATCAAC73700.026.4600622
TCAACGC74200.026.2113464
CAACGCA74800.026.0010955
AACGCAG76350.025.4426126
GTACATG50700.024.8606471
GGTCGAC1000.001654880523.7474757
TACATGG52450.023.4870662
ACGCAGA84450.022.7773287
GTATAGC6150.022.4283541
CGCAGAG86800.022.1606628
ACATGGG53750.021.764143
GAGTACT51950.021.71328212-13
GGACCGA2657.9489837E-1021.5071496
GTACTTT53100.021.1983114-15
AGGACCG3850.020.9970425
AGTACTT53300.020.80688912-13
GCAGAGT93150.020.4460289
Produced by FastQC (version 0.11.5)