##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938199_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1455480 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45656621870448 33.0 31.0 34.0 31.0 34.0 2 32.68296987935251 34.0 31.0 34.0 31.0 34.0 3 32.75130197598043 34.0 31.0 34.0 31.0 34.0 4 36.21490298733064 37.0 35.0 37.0 35.0 37.0 5 36.13857284194905 37.0 35.0 37.0 35.0 37.0 6 36.19367562591035 37.0 37.0 37.0 35.0 37.0 7 36.16255187292165 37.0 37.0 37.0 35.0 37.0 8 36.16118050402616 37.0 37.0 37.0 35.0 37.0 9 37.845596641657735 39.0 38.0 39.0 35.0 39.0 10-11 37.80976722455822 39.0 38.0 39.0 35.0 39.0 12-13 37.72271999615248 39.0 37.5 39.0 35.0 39.0 14-15 38.95720209140627 40.0 38.0 41.0 35.5 41.0 16-17 38.86294624453788 40.0 38.0 41.0 35.0 41.0 18-19 39.10858273559238 40.0 38.5 41.0 36.0 41.0 20-21 39.082197625525595 40.0 39.0 41.0 36.0 41.0 22-23 38.9720975210927 40.0 38.5 41.0 35.0 41.0 24-25 38.86168686618848 40.0 38.0 41.0 35.0 41.0 26-27 38.70007111056147 40.0 38.0 41.0 34.5 41.0 28-29 38.57533734575536 40.0 38.0 41.0 34.0 41.0 30-31 38.33634264984747 40.0 38.0 41.0 34.0 41.0 32-33 38.12750638964465 40.0 38.0 41.0 34.0 41.0 34-35 38.04260862395911 40.0 38.0 41.0 33.0 41.0 36-37 37.99580207216863 40.0 38.0 41.0 33.0 41.0 38-39 37.813258169126335 40.0 37.0 41.0 33.0 41.0 40-41 37.59924011322725 40.0 37.0 41.0 33.0 41.0 42-43 37.34646611427158 40.0 37.0 41.0 32.0 41.0 44-45 37.07205767169594 40.0 36.0 41.0 31.0 41.0 46-47 36.705995616566355 39.0 36.0 41.0 30.5 41.0 48-49 36.308230961607165 39.0 35.0 41.0 30.0 41.0 50-51 35.92412640503477 39.0 35.0 40.0 29.0 41.0 52-53 35.383959930744496 38.0 35.0 40.0 27.5 41.0 54-55 34.99738780333635 38.0 34.0 40.0 26.5 41.0 56-57 34.60898741308709 38.0 34.0 40.0 26.0 41.0 58-59 34.09426752686399 37.5 33.0 40.0 24.5 41.0 60-61 33.58749450353148 37.0 33.0 39.5 22.5 41.0 62-63 33.01433719460247 36.0 32.0 39.0 21.5 40.0 64-65 32.429686082941714 36.0 31.0 39.0 19.0 40.0 66-67 31.84456090087119 35.0 31.0 38.5 16.0 40.0 68-69 31.20001820705197 35.0 30.0 38.0 10.0 40.0 70-71 30.562636037595844 34.5 29.0 37.0 7.5 39.5 72-73 29.862550155275237 34.0 28.5 36.5 4.0 39.0 74-75 29.182297249017505 34.0 28.0 36.0 2.0 39.0 76-77 27.894701060818424 32.0 26.5 34.5 2.0 36.5 78-79 28.543540618902355 33.5 28.0 35.0 2.0 37.0 80-81 28.238129689174706 34.0 27.0 35.0 2.0 37.0 82-83 27.662340258883667 33.0 26.0 35.0 2.0 36.0 84-85 26.98258547008547 33.0 25.5 35.0 2.0 36.0 86-87 26.235392791381535 32.0 24.5 35.0 2.0 35.5 88-89 25.41776596037046 32.0 20.5 34.0 2.0 35.0 90-91 24.739291161678622 31.0 18.5 34.0 2.0 35.0 92-93 23.76541312831506 31.0 6.0 34.0 2.0 35.0 94-95 22.831654505730068 30.5 2.0 34.0 2.0 35.0 96-97 21.75082447027785 29.5 2.0 34.0 2.0 35.0 98-99 20.322180311649767 29.0 2.0 34.0 2.0 35.0 100-101 16.535979882925222 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1735470874637457 1101 2 0.22551373052480272 1101 3 0.19858946691481094 1101 4 0.12035999680589526 1101 5 0.032377720184051384 1101 6 0.14933218725931852 1101 7 0.13730726257900017 1101 8 0.16670187620215415 1101 9 0.02838558016910042 1101 10-11 0.11111556297029068 1101 12-13 0.19629937685865428 1101 14-15 0.35610948891073946 1101 16-17 0.3946386921442553 1101 18-19 0.32231910911853845 1101 20-21 0.3337748313614952 1101 22-23 0.39185126704660433 1101 24-25 0.4507888969565954 1101 26-27 0.4080153544370688 1101 28-29 0.39649923500167716 1101 30-31 0.38657333646801817 1101 32-33 0.5090576661821729 1101 34-35 0.3296418689027689 1101 36-37 0.35340169662868703 1101 38-39 0.6040841656779818 1101 40-41 0.5344852755748875 1101 42-43 0.6382954447811287 1101 44-45 0.6435330488710349 1101 46-47 0.9504619436052337 1101 48-49 1.001155146105674 1101 50-51 0.9733095353737085 1101 52-53 1.003568167282431 1101 54-55 1.2351131303783447 1101 56-57 1.1742246795828208 1101 58-59 1.4858558203828451 1101 60-61 1.604272372856414 1101 62-63 1.525364664376987 1101 64-65 1.6043623397895388 1101 66-67 1.9093087248800806 1101 68-69 2.211643302785607 1101 70-71 2.3699618947982124 1101 72-73 2.439423863209509 1101 74-75 2.2620192801904757 1101 76-77 2.3878156068507224 1101 78-79 2.5523491169005226 1101 80-81 2.6539490543922035 1101 82-83 2.8992444269302595 1101 84-85 2.8276137753428294 1101 86-87 2.8345726229733472 1101 88-89 3.287417220115966 1101 90-91 3.6697992506164425 1101 92-93 4.0384520907388755 1101 94-95 4.072918006414344 1101 96-97 4.1247334283672 1101 98-99 4.1995562208404 1101 100-101 4.133949119477496 1102 1 0.23939420483085172 1102 2 0.21720145433772586 1102 3 0.2104145401626738 1102 4 0.10304094616023463 1102 5 0.05928332732746355 1102 6 0.08589411077149833 1102 7 0.1341665181636671 1102 8 0.13772827643779095 1102 9 0.12272572930019976 1102 10-11 0.21572591533514895 1102 12-13 0.24423991044415772 1102 14-15 0.38605817665006015 1102 16-17 0.35804731446431504 1102 18-19 0.32084175007223337 1102 20-21 0.30739629879913366 1102 22-23 0.3484497757355882 1102 24-25 0.34385631599638344 1102 26-27 0.4349070096062775 1102 28-29 0.32042066988471873 1102 30-31 0.4159546439230226 1102 32-33 0.4588941180401065 1102 34-35 0.429364379638038 1102 36-37 0.42026420767399486 1102 38-39 0.4350683999471343 1102 40-41 0.6277619742277452 1102 42-43 0.5687262507401769 1102 44-45 0.6142711083064469 1102 46-47 0.7118118746204729 1102 48-49 0.8935172153384769 1102 50-51 0.9713423070108931 1102 52-53 1.0064283219943277 1102 54-55 1.2772232889657786 1102 56-57 1.2783544639473092 1102 58-59 1.40058445448458 1102 60-61 1.4468398461538925 1102 62-63 1.532258489853291 1102 64-65 1.7471297913839337 1102 66-67 1.7381319533669135 1102 68-69 2.0522621503525365 1102 70-71 2.301657679751788 1102 72-73 2.467861087076688 1102 74-75 2.394456069996231 1102 76-77 2.3820704939172437 1102 78-79 2.515604267504333 1102 80-81 2.805670572987083 1102 82-83 2.8577760591562154 1102 84-85 3.2406340677142005 1102 86-87 3.40426428434343 1102 88-89 3.614101145891464 1102 90-91 3.7287788962362995 1102 92-93 3.6854314883236334 1102 94-95 3.8611177366761744 1102 96-97 4.22890816909301 1102 98-99 4.767899191370731 1102 100-101 4.276321265136108 1103 1 0.1600786963000118 1103 2 0.13141971637482897 1103 3 0.11142168814465947 1103 4 0.09757858991623891 1103 5 0.038188295785239745 1103 6 -0.009222576089733536 1103 7 0.15516330588854288 1103 8 0.10646173500717993 1103 9 0.16732484986918195 1103 10-11 0.1959169846744473 1103 12-13 0.214773910247672 1103 14-15 0.4178073036197745 1103 16-17 0.4341175022290855 1103 18-19 0.3328593506009625 1103 20-21 0.3396250342225784 1103 22-23 0.32296354326690135 1103 24-25 0.3588420706428721 1103 26-27 0.42497277342218354 1103 28-29 0.4572600174266839 1103 30-31 0.40346338911131596 1103 32-33 0.45733799551518217 1103 34-35 0.5048129519767528 1103 36-37 0.4994805792618848 1103 38-39 0.5155446740652465 1103 40-41 0.572428376926176 1103 42-43 0.6317508503966209 1103 44-45 0.6969560820634442 1103 46-47 0.7030863350981704 1103 48-49 0.639350337794923 1103 50-51 0.81104352672277 1103 52-53 0.8786795046414895 1103 54-55 0.89488871815729 1103 56-57 1.0917445008333928 1103 58-59 1.1964951300150162 1103 60-61 1.2578392834847563 1103 62-63 1.5195662817788431 1103 64-65 1.4245286523510785 1103 66-67 1.468859046927772 1103 68-69 1.6914227313605217 1103 70-71 1.6903626178432951 1103 72-73 1.7044372602716074 1103 74-75 1.6573374718461942 1103 76-77 1.6634369165621443 1103 78-79 1.9043437397861815 1103 80-81 2.1067170184960027 1103 82-83 2.135130263842747 1103 84-85 2.3435539930489035 1103 86-87 2.4426776169225697 1103 88-89 2.5997870217053247 1103 90-91 2.9072646910734363 1103 92-93 3.333381020772375 1103 94-95 3.4990466974649834 1103 96-97 3.540748633041993 1103 98-99 3.7917438589642636 1103 100-101 3.7799146071823735 1104 1 0.2690538023261482 1104 2 0.1865426098566303 1104 3 0.17735578501254423 1104 4 0.11186066081652513 1104 5 0.02442249179664202 1104 6 0.12323788895584187 1104 7 0.17165288230759046 1104 8 0.15532017709717394 1104 9 0.2639906213729404 1104 10-11 0.23596539103111525 1104 12-13 0.25722095910948894 1104 14-15 0.435784314259827 1104 16-17 0.3819069846841714 1104 18-19 0.3731301401965794 1104 20-21 0.26923651662924897 1104 22-23 0.36825118613405294 1104 24-25 0.40895740027958283 1104 26-27 0.38507693885729566 1104 28-29 0.419184065950148 1104 30-31 0.33775111248683487 1104 32-33 0.49088605534809915 1104 34-35 0.5532601258235772 1104 36-37 0.5997962508620773 1104 38-39 0.6681443341285345 1104 40-41 0.6185053395427147 1104 42-43 0.7938454302696414 1104 44-45 0.919212084018163 1104 46-47 0.9536703022237418 1104 48-49 1.1422458238655722 1104 50-51 1.1324976541275333 1104 52-53 1.2350185247927854 1104 54-55 1.2297072337294637 1104 56-57 1.1833466904356555 1104 58-59 1.4020524027069783 1104 60-61 1.5758867248670612 1104 62-63 1.5723426098430977 1104 64-65 1.6212238122221052 1104 66-67 1.781762398046137 1104 68-69 1.7536914457545656 1104 70-71 1.8905855077782299 1104 72-73 2.208551913187666 1104 74-75 2.1136467658328932 1104 76-77 2.1663101643088964 1104 78-79 2.185251209779924 1104 80-81 2.3703106865977475 1104 82-83 2.565620863787686 1104 84-85 2.9105186532557603 1104 86-87 2.8769467614452147 1104 88-89 3.0247495684269197 1104 90-91 3.102275086786115 1104 92-93 3.3389997162779252 1104 94-95 3.573212633644932 1104 96-97 3.974964292988183 1104 98-99 3.948988513743842 1104 100-101 3.8374471215823966 1105 1 0.18845911565156825 1105 2 0.18069474058859925 1105 3 0.17918154945171239 1105 4 0.10439437950631003 1105 5 0.05525845159367293 1105 6 0.057405097928779014 1105 7 0.16631435559422414 1105 8 0.08433653985966316 1105 9 0.2371113706487975 1105 10-11 0.15755667712638655 1105 12-13 0.18838421669008198 1105 14-15 0.3412478372514798 1105 16-17 0.28012593392856644 1105 18-19 0.3009223263075853 1105 20-21 0.23445793708886953 1105 22-23 0.17981012525539342 1105 24-25 0.25237417434414766 1105 26-27 0.2902695745508339 1105 28-29 0.23864366306975882 1105 30-31 0.3621487803572023 1105 32-33 0.4156120843476572 1105 34-35 0.5211279477089334 1105 36-37 0.47782279834897423 1105 38-39 0.48461406918534067 1105 40-41 0.512375736851169 1105 42-43 0.35444097825417487 1105 44-45 0.46167838659528826 1105 46-47 0.4520074955016966 1105 48-49 0.4279229304595873 1105 50-51 0.5881795351434107 1105 52-53 0.6859047170371682 1105 54-55 0.764794981010489 1105 56-57 0.7499610404975385 1105 58-59 0.9884808693200497 1105 60-61 0.9899346370942652 1105 62-63 1.2107995145715833 1105 64-65 1.343677046281492 1105 66-67 1.288364179362727 1105 68-69 1.5173798939930272 1105 70-71 1.9827762443364811 1105 72-73 1.9268009444798047 1105 74-75 1.854815148739398 1105 76-77 1.7300521519996082 1105 78-79 1.7467250812512134 1105 80-81 1.8581305941292108 1105 82-83 2.154205795329492 1105 84-85 2.628692984517695 1105 86-87 2.8424348158204573 1105 88-89 2.7041022142435054 1105 90-91 2.9948657476341545 1105 92-93 3.22980661483901 1105 94-95 3.4967602098549158 1105 96-97 3.6605119904195647 1105 98-99 3.6734211656852978 1105 100-101 3.2525743296195877 1106 1 0.12618990662251406 1106 2 0.0693998562574194 1106 3 0.09496115361554303 1106 4 0.05475859086648427 1106 5 -0.044287496181041774 1106 6 -0.04994493693484259 1106 7 0.15160097992944088 1106 8 0.059784506423596895 1106 9 0.08170833483210771 1106 10-11 0.1256515659960158 1106 12-13 0.07905273174745275 1106 14-15 0.008683205464940613 1106 16-17 0.028450056252417255 1106 18-19 -0.11180335889662274 1106 20-21 -0.11573448327148128 1106 22-23 -0.09055543732790028 1106 24-25 -0.12324444860477257 1106 26-27 -0.2327378355154721 1106 28-29 -0.06055761966387507 1106 30-31 0.030975598938475457 1106 32-33 0.13993600862964684 1106 34-35 0.08324501520407779 1106 36-37 0.28533307177051626 1106 38-39 0.08509658992816327 1106 40-41 0.2718072226189676 1106 42-43 0.1551672864650726 1106 44-45 0.3615955597127538 1106 46-47 0.5192904127408227 1106 48-49 0.651977125852639 1106 50-51 0.7810166085798116 1106 52-53 0.8537369425738461 1106 54-55 0.9197023964404281 1106 56-57 1.0577552254933948 1106 58-59 1.0965681459556507 1106 60-61 1.1416762192836956 1106 62-63 1.231749403791916 1106 64-65 1.627955049606939 1106 66-67 1.6597138140167509 1106 68-69 1.4288572450180972 1106 70-71 1.3346643981324853 1106 72-73 1.7239374860912875 1106 74-75 1.8957205920024691 1106 76-77 1.6478717420529527 1106 78-79 1.9834054767573122 1106 80-81 1.8612884334925042 1106 82-83 2.199390025833715 1106 84-85 2.2673648353426614 1106 86-87 2.432975016255323 1106 88-89 2.752826958748873 1106 90-91 2.7332104168011 1106 92-93 2.7939554164794522 1106 94-95 3.0062311492747362 1106 96-97 3.681888391668881 1106 98-99 3.8223452929602004 1106 100-101 3.7536359680859164 1107 1 0.20822919838330733 1107 2 0.17400572492059752 1107 3 0.22034037932883166 1107 4 0.20472124933557723 1107 5 0.05844768057404082 1107 6 0.10770708131551032 1107 7 0.1392958350618585 1107 8 0.15272824828257114 1107 9 0.21627368044527628 1107 10-11 0.2172271513490429 1107 12-13 0.26135377576088104 1107 14-15 0.4003202003342281 1107 16-17 0.4286828339561666 1107 18-19 0.2624638845753253 1107 20-21 0.2823314010307172 1107 22-23 0.39640007615172124 1107 24-25 0.37629872450602164 1107 26-27 0.4447756936878733 1107 28-29 0.46018371560315785 1107 30-31 0.4373785094086742 1107 32-33 0.5152096141068228 1107 34-35 0.5114849740509229 1107 36-37 0.5686483799388569 1107 38-39 0.5578220361930306 1107 40-41 0.7133876112394617 1107 42-43 0.6348259652463 1107 44-45 0.8923173616921147 1107 46-47 0.9696491600073571 1107 48-49 0.9232494029422966 1107 50-51 0.8150957912186314 1107 52-53 1.0413458553723345 1107 54-55 1.137866804538426 1107 56-57 1.1231865744058425 1107 58-59 1.3732446154197788 1107 60-61 1.4054373223204948 1107 62-63 1.3316728614007545 1107 64-65 1.3415894083604059 1107 66-67 1.570073480618344 1107 68-69 1.8607458794543312 1107 70-71 1.914163381182636 1107 72-73 2.075261121989154 1107 74-75 1.9529628863735553 1107 76-77 2.0385253582935796 1107 78-79 2.099573533272661 1107 80-81 2.243618452054811 1107 82-83 2.5686262340000994 1107 84-85 2.5308782860409984 1107 86-87 2.7116910667092604 1107 88-89 2.8509045155074837 1107 90-91 3.0300303955014485 1107 92-93 3.187615206726445 1107 94-95 3.3696197666671104 1107 96-97 3.533845748983431 1107 98-99 3.772668403081049 1107 100-101 3.7621310372478014 1108 1 0.19982103099094672 1108 2 0.21837953078809136 1108 3 0.1700167128075094 1108 4 0.15188977289803773 1108 5 -0.06916835171467284 1108 6 0.114600662122605 1108 7 0.06536968537689347 1108 8 0.12624826125872346 1108 9 0.13013288498187592 1108 10-11 0.175622890284032 1108 12-13 0.09392981580157311 1108 14-15 0.3166408713866602 1108 16-17 0.3489237353774328 1108 18-19 0.2829108034764829 1108 20-21 0.18882885109043457 1108 22-23 0.2625150457019956 1108 24-25 0.23586663549298947 1108 26-27 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1.7072761442902014 2316 74-75 2.026755778389024 2316 76-77 2.0955677418188223 2316 78-79 2.171326283405346 2316 80-81 2.202991154783348 2316 82-83 2.441163311755421 2316 84-85 2.620130269729753 2316 86-87 2.6131877751210197 2316 88-89 2.8929831114481317 2316 90-91 3.4532125223586334 2316 92-93 3.360721101257166 2316 94-95 3.924838343290073 2316 96-97 4.492978216866959 2316 98-99 4.505026594471161 2316 100-101 4.91027888016141 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 5.0 5 23.0 6 51.0 7 163.0 8 341.0 9 672.0 10 1113.0 11 2026.0 12 2987.0 13 4557.0 14 6099.0 15 8411.0 16 10949.0 17 15320.0 18 15994.0 19 13735.0 20 13510.0 21 14618.0 22 16031.0 23 17803.0 24 19940.0 25 22125.0 26 25157.0 27 29027.0 28 34737.0 29 41275.0 30 48897.0 31 58217.0 32 70543.0 33 86864.0 34 113226.0 35 153060.0 36 207885.0 37 258088.0 38 136857.0 39 5170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02367603814549 18.978275208178747 14.86595487399346 23.1320938796823 2 15.708979966261136 20.020614377153773 39.9967016996554 24.273703956929698 3 19.66788027529838 23.684331669001587 29.68616341873483 26.961624636965205 4 13.178439977106784 16.89563318087221 36.334598231615864 33.59132861040515 5 14.039890523576018 36.868847711801045 34.051872675534746 15.039389089088196 6 32.397925356532106 35.793096293385204 17.17146039207711 14.637517958005578 7 28.92379146398439 32.32486877181411 20.1401599472339 18.6111798169676 8 26.155118799459697 35.42891063966614 19.26858392912844 19.14738663174572 9 25.92732294500783 17.161348833374557 20.25338719872482 36.65794102289279 10-11 25.32425728969137 26.58902904883612 26.703493005743812 21.383220655728692 12-13 25.976619397037403 23.785211751449694 27.49189270893451 22.74627614257839 14-15 22.717316624069035 25.32511611289746 25.86713661472504 26.09043064830846 16-17 22.31418501113035 28.625367576332206 26.543683183554567 22.51676422898288 18-19 22.116071673949484 27.089791683843128 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22.525715341875514 44-45 22.37294133005366 26.53614395745587 28.699147331029316 22.39176738146115 46-47 22.45815813339929 26.83104542831231 28.255283480363868 22.455512957924533 48-49 22.4618682496496 26.770481215818837 28.22862560804683 22.539024926484732 50-51 22.398590155824884 27.008684420259982 28.146144227333934 22.446581196581196 52-53 22.706151922389864 26.938741858356007 27.913025256272846 22.442080962981283 54-55 22.361523346250035 26.89397999285459 28.06788825679501 22.676608404100364 56-57 22.433389672135654 27.116449556160166 27.997705224393325 22.452455547310855 58-59 22.635110066782094 26.815140022535523 27.976406408882294 22.573343501800096 60-61 22.503607057465576 26.88786517162723 28.20959408579987 22.39893368510732 62-63 22.35296946711738 27.050320169291233 28.08729766125265 22.509412702338746 64-65 22.170280594717894 26.864951768488748 28.069159315140023 22.895608321653338 66-67 22.196446048498828 27.082742891770646 27.880384354698474 22.84042670503206 68-69 22.268839434107583 26.874942887903643 27.963216031099215 22.893001646889562 70-71 22.30350125044659 26.953925852639678 27.79220600764009 22.950366889273642 72-73 22.41872867326541 26.96056522953432 27.739792968571525 22.880913128628745 74-75 22.333972297798667 27.19786599609751 27.443042844972105 23.025118861131723 76-77 22.311849012009784 26.98422513535054 27.780010718113612 22.92391513452607 78-79 22.31191771786627 27.089242036991234 27.693303927226758 22.90553631791574 80-81 22.30104316173576 27.338388255687647 27.529734197470386 22.830834385106208 82-83 22.428576385298467 27.154560417079626 27.62771191247264 22.789151285149266 84-85 22.368647066502685 27.066227850188607 27.639426148973158 22.925698934335557 86-87 22.428280353418533 26.94625651391691 27.700804188969464 22.924658943695093 88-89 22.28553466897518 27.107552147745075 27.398796273394343 23.208116909885398 90-91 22.331161719500415 27.132885727124194 27.580480485019716 22.95547206835568 92-93 22.442630609833184 26.869795531371093 27.582309616071676 23.10526424272405 94-95 22.25757138538489 26.923386099431116 27.47990353697749 23.339138978206503 96-97 22.32373144767561 26.857053146801256 27.50003778827299 23.31917761725014 98-99 22.24317750845082 26.79768873498777 27.299550663698575 23.659583092862835 100-101 22.630300656827988 26.389541594525518 27.380864044851183 23.59929370379531 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1061.0 1 687.0 2 459.5 3 606.0 4 472.5 5 404.5 6 798.5 7 1762.0 8 2649.0 9 2948.5 10 2429.0 11 1729.5 12 1621.0 13 1737.5 14 1821.5 15 1863.0 16 1948.0 17 2011.5 18 2077.5 19 2261.0 20 2537.5 21 2808.0 22 3294.0 23 4160.5 24 5096.5 25 6092.0 26 7524.5 27 9387.0 28 11204.0 29 12944.0 30 15129.0 31 17379.0 32 19679.5 33 21968.0 34 24338.5 35 26853.0 36 29842.0 37 33125.0 38 35953.5 39 37849.5 40 39553.5 41 43070.5 42 46561.5 43 49175.0 44 51574.5 45 53989.5 46 55426.5 47 56992.5 48 60427.5 49 60878.5 50 58978.0 51 56958.0 52 54438.5 53 52935.5 54 51139.5 55 47130.0 56 42243.0 57 38277.0 58 34336.0 59 29883.5 60 24833.0 61 19928.0 62 16000.0 63 12457.5 64 9661.5 65 7521.0 66 5672.5 67 4599.5 68 3596.0 69 2488.0 70 1785.0 71 1308.5 72 1038.5 73 809.0 74 591.0 75 481.5 76 305.5 77 178.5 78 115.0 79 70.5 80 35.5 81 16.0 82 11.5 83 10.5 84 7.5 85 3.5 86 2.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01271058345013329 3 0.0028169401159754857 4 0.0018550581251545882 5 0.1134333690603787 6 6.870585648720696E-5 7 0.0 8 1.3741171297441393E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.717646412180174E-4 24-25 2.404704977052244E-4 26-27 0.0053590568060021436 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010168466760106631 36-37 4.1223513892324183E-4 38-39 0.006355291725066645 40-41 0.008966114271580509 42-43 0.00511858630829692 44-45 0.003435292824360348 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.0305878473081046E-4 68-69 6.183527083848628E-4 70-71 0.0 72-73 3.435292824360348E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.870585648720696E-5 82-83 0.0018207051969109846 84-85 0.003435292824360348 86-87 0.0026451754747574686 88-89 0.0 90-91 0.0023359991205650367 92-93 0.0 94-95 0.0 96-97 1.3741171297441393E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1455480.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.076020604226926 #Duplication Level Percentage of deduplicated Percentage of total 1 81.76289308140953 51.572779286641904 2 10.984702795337082 13.857426796999817 3 3.120759714516888 5.905353121611257 4 1.3150482266589052 3.317920361611567 5 0.7145735705004075 2.2536228628059853 6 0.4306862240487685 1.629958388523409 7 0.2934470898281147 1.2956632278974014 8 0.21536994286852573 1.0867743163125043 9 0.15465852955349557 0.8779720137062152 >10 0.9135134708357079 10.407378306202492 >50 0.06500720123516476 2.7908055880234204 >100 0.02726785177019085 3.1721688613095504 >500 0.0014178904570952317 0.5362653457803985 >1k 4.3627398679853285E-4 0.4888648553066725 >5k 2.1813699339926642E-4 0.8070466672674605 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6252 0.429549014758018 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5479 0.376439387693407 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3509 0.24108885041360925 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1535 0.10546348970786269 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.0305878473081044E-4 0.0 22-23 0.0 0.0 0.0 2.0611756946162092E-4 0.0 24-25 0.0 0.0 0.0 4.4658806716684526E-4 0.0 26-27 0.0 0.0 0.0 0.001133646632038915 0.0 28-29 0.0 0.0 0.0 0.0029543518289498996 0.0 30-31 0.0 0.0 0.0 0.00955011405172177 0.0 32-33 0.0 0.0 0.0 0.024356226124714872 0.0 34-35 0.0 0.0 0.0 0.04671998241130074 6.870585648720696E-5 36-37 0.0 0.0 0.0 0.08165691043504548 6.870585648720696E-5 38-39 0.0 0.0 0.0 0.13572841949047737 6.870585648720696E-5 40-41 0.0 0.0 0.0 0.19673922005111716 6.870585648720696E-5 42-43 0.0 0.0 0.0 0.2564446093385 6.870585648720696E-5 44-45 0.0 0.0 0.0 0.32892928793250337 6.870585648720696E-5 46-47 0.0 0.0 0.0 0.41759419572924394 6.870585648720696E-5 48-49 0.0 0.0 0.0 0.5091103965702036 6.870585648720696E-5 50-51 0.0 0.0 0.0 0.6029282436034847 6.870585648720696E-5 52-53 0.0 0.0 0.0 0.6924519746063154 6.870585648720696E-5 54-55 0.0 0.0 0.0 0.7892928793250337 1.0305878473081044E-4 56-57 0.0 0.0 0.0 0.9040660125869129 1.3741171297441393E-4 58-59 0.0 0.0 0.0 1.0247822024349356 1.3741171297441393E-4 60-61 0.0 0.0 0.0 1.1555638002583342 1.3741171297441393E-4 62-63 0.0 0.0 0.0 1.2842498694588727 1.3741171297441393E-4 64-65 0.0 0.0 0.0 1.4152032319234893 1.3741171297441393E-4 66-67 0.0 0.0 0.0 1.5688638799571275 1.3741171297441393E-4 68-69 0.0 0.0 0.0 1.7350976997279248 1.3741171297441393E-4 70-71 0.0 0.0 0.0 1.909301398851238 1.3741171297441393E-4 72-73 0.0 0.0 0.0 2.0827149806249485 1.3741171297441393E-4 74-75 0.0 0.0 0.0 2.263548794899277 1.3741171297441393E-4 76-77 0.0 0.0 0.0 2.4527990765932888 1.3741171297441393E-4 78-79 0.0 0.0 0.0 2.658298293346525 1.3741171297441393E-4 80-81 0.0 0.0 0.0 2.8867796190947317 1.717646412180174E-4 82-83 0.0 0.0 0.0 3.1216849424244923 2.0611756946162092E-4 84-85 0.0 0.0 0.0 3.3540481490642264 2.0611756946162092E-4 86-87 0.0 0.0 0.0 3.597163822244208 2.0611756946162092E-4 88-89 0.0 0.0 0.0 3.8604103113749417 2.0611756946162092E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3735 0.0 44.188915 1 GTATCAA 5745 0.0 34.35837 1 ATCAACG 7220 0.0 26.938349 3 TATCAAC 7370 0.0 26.460062 2 TCAACGC 7420 0.0 26.211346 4 CAACGCA 7480 0.0 26.001095 5 AACGCAG 7635 0.0 25.442612 6 GTACATG 5070 0.0 24.860647 1 GGTCGAC 100 0.0016548805 23.747475 7 TACATGG 5245 0.0 23.487066 2 ACGCAGA 8445 0.0 22.777328 7 GTATAGC 615 0.0 22.428354 1 CGCAGAG 8680 0.0 22.160662 8 ACATGGG 5375 0.0 21.76414 3 GAGTACT 5195 0.0 21.713282 12-13 GGACCGA 265 7.9489837E-10 21.507149 6 GTACTTT 5310 0.0 21.19831 14-15 AGGACCG 385 0.0 20.997042 5 AGTACTT 5330 0.0 20.806889 12-13 GCAGAGT 9315 0.0 20.446028 9 >>END_MODULE