FastQCFastQC Report
Thu 26 May 2016
SRR938198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457126
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66930.45932884321602935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55700.38225932417649533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34870.23930669001857083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42050.039.7105331
GTATCAA63500.034.4859851
ATCAACG77550.027.5740533
TATCAAC78600.027.3861312
TCAACGC78950.027.265664
CAACGCA79850.026.958345
AACGCAG81400.026.4413766
GTACATG51650.024.5705571
TACATGG51350.024.0595722
ACGCAGA90000.023.592337
CGCAGAG91300.023.1523518
GAGTACT51350.022.8495312-13
ACATGGG52700.022.8128643
GTACCTG10300.021.7702961
GCAGAGT96250.021.517499
GTACTTT55000.021.46307414-15
AGAGTAC79100.020.77808210-11
AGTACTT55100.019.61329512-13
TACCTGG10500.019.459612
CAGAGTA93450.019.44276210-11
Produced by FastQC (version 0.11.5)