##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938198_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1457126 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.825973869109465 33.0 31.0 34.0 30.0 34.0 2 32.01600410671418 34.0 31.0 34.0 30.0 34.0 3 32.096849551788935 34.0 31.0 34.0 30.0 34.0 4 35.61511152776081 37.0 35.0 37.0 33.0 37.0 5 35.61563722011686 37.0 35.0 37.0 33.0 37.0 6 35.63879238995118 37.0 35.0 37.0 33.0 37.0 7 35.57942621296991 37.0 35.0 37.0 33.0 37.0 8 35.556933991981474 37.0 35.0 37.0 33.0 37.0 9 37.18412340456488 39.0 37.0 39.0 34.0 39.0 10-11 37.08846969994359 39.0 37.0 39.0 33.0 39.0 12-13 36.97767385936426 39.0 37.0 39.0 33.0 39.0 14-15 38.14966619221673 40.0 38.0 41.0 33.0 41.0 16-17 37.98964399784233 40.0 38.0 41.0 33.0 41.0 18-19 38.12969708865259 40.0 38.0 41.0 33.0 41.0 20-21 38.1149173098277 40.0 38.0 41.0 33.0 41.0 22-23 38.01674769374783 40.0 38.0 41.0 33.0 41.0 24-25 37.89957388722732 40.0 38.0 41.0 33.0 41.0 26-27 37.723896903905356 40.0 38.0 41.0 32.0 41.0 28-29 37.50328386151918 40.0 37.0 41.0 32.0 41.0 30-31 37.29475385107396 40.0 37.0 41.0 31.5 41.0 32-33 37.04644313532255 40.0 36.5 41.0 31.0 41.0 34-35 36.81287170773152 39.5 36.0 41.0 30.0 41.0 36-37 36.55111363053024 39.0 35.5 41.0 30.0 41.0 38-39 36.26550895392711 39.0 35.0 40.5 30.0 41.0 40-41 36.04768908110898 39.0 35.0 40.0 29.0 41.0 42-43 35.85728207443968 39.0 35.0 40.0 28.5 41.0 44-45 35.577834037687886 39.0 35.0 40.0 27.0 41.0 46-47 35.19259315941106 38.5 35.0 40.0 25.5 41.0 48-49 34.80800699459073 38.0 34.0 40.0 24.0 41.0 50-51 34.12355829214495 37.5 33.5 39.5 23.5 40.5 52-53 34.27123152012935 38.0 34.0 40.0 24.0 40.5 54-55 34.37063678775892 38.0 34.0 40.0 23.0 41.0 56-57 34.05892764249626 38.0 34.0 40.0 22.5 41.0 58-59 33.72906872844215 38.0 33.0 40.0 21.5 41.0 60-61 33.293989675566834 37.0 33.0 40.0 19.0 41.0 62-63 32.737345294778905 36.5 32.0 39.5 15.5 41.0 64-65 32.18945890746579 36.0 31.0 39.0 10.0 40.5 66-67 31.629988758693482 35.5 31.0 39.0 7.5 40.0 68-69 30.9969755532466 35.0 30.0 38.0 3.5 40.0 70-71 30.407375546109257 35.0 29.5 37.5 2.0 40.0 72-73 29.727754154410803 34.0 29.0 37.0 2.0 39.0 74-75 29.007911807215024 34.0 27.5 36.0 2.0 39.0 76-77 28.304253029593873 34.0 26.5 36.0 2.0 38.5 78-79 27.53877049754105 33.0 26.0 35.0 2.0 37.0 80-81 26.83924073827521 33.0 24.5 35.0 2.0 37.0 82-83 26.079143464600868 32.0 22.0 35.0 2.0 36.0 84-85 25.38213819532422 32.0 19.5 35.0 2.0 36.0 86-87 24.64816117480575 31.0 16.5 34.0 2.0 35.0 88-89 23.80373625890966 31.0 7.0 34.0 2.0 35.0 90-91 22.933390454909187 30.0 2.0 34.0 2.0 35.0 92-93 21.992262165385835 29.5 2.0 34.0 2.0 35.0 94-95 21.00033593525886 29.0 2.0 34.0 2.0 35.0 96-97 19.759737318529766 27.5 2.0 34.0 2.0 35.0 98-99 18.258721963646245 25.0 2.0 33.0 2.0 35.0 100-101 14.691034955110265 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1997134463693584 1101 2 0.23190674109871878 1101 3 0.15207155170534747 1101 4 0.14734561819751946 1101 5 0.1569118236675564 1101 6 0.1773752840033822 1101 7 0.2099871482397475 1101 8 0.06510927459479632 1101 9 0.19079232572343585 1101 10-11 0.15839277414353603 1101 12-13 0.3369719663561668 1101 14-15 0.39347175287305447 1101 16-17 0.3641240790658671 1101 18-19 0.4052224708590657 1101 20-21 0.4404362572350706 1101 22-23 0.4900736765718321 1101 24-25 0.39510400671246515 1101 26-27 0.4942821019743846 1101 28-29 0.6156890428704358 1101 30-31 0.6236755966041727 1101 32-33 0.6012338384091507 1101 34-35 0.669705486826949 1101 36-37 0.67428798118668 1101 38-39 0.6766925883989927 1101 40-41 0.693963082479307 1101 42-43 0.9379785301009989 1101 44-45 0.912350482092954 1101 46-47 0.9649151099474054 1101 48-49 1.1197848497871519 1101 50-51 1.0621276679533622 1101 52-53 1.1105128423992028 1101 54-55 1.1375097022713234 1101 56-57 1.0873637697198433 1101 58-59 1.126047211065007 1101 60-61 1.1297105746730267 1101 62-63 1.1891661044991224 1101 64-65 1.0539676367192925 1101 66-67 1.240212464534519 1101 68-69 1.3657007445265457 1101 70-71 1.756749775079495 1101 72-73 1.9886946828214 1101 74-75 2.2871103074367554 1101 76-77 2.289947260211317 1101 78-79 2.25121660832475 1101 80-81 2.43274879904828 1101 82-83 2.630008510727329 1101 84-85 2.8252966571794076 1101 86-87 3.0472558097467335 1101 88-89 3.2429484653276397 1101 90-91 3.3070791764398315 1101 92-93 3.500416209898898 1101 94-95 3.976345911088316 1101 96-97 4.0907749241264355 1101 98-99 4.459573083526937 1101 100-101 4.250743457861871 1102 1 0.42761494075861606 1102 2 0.3026520560628043 1102 3 0.19747160417931298 1102 4 0.1628612420699298 1102 5 0.2514192312800887 1102 6 0.3984344637871473 1102 7 0.1508017702397666 1102 8 0.27986598390634754 1102 9 0.39657251797508053 1102 10-11 0.4674960566755715 1102 12-13 0.49763517466383433 1102 14-15 0.5766282802599392 1102 16-17 0.6143793162532418 1102 18-19 0.501416262036166 1102 20-21 0.6103817078035334 1102 22-23 0.5760535048622373 1102 24-25 0.4816494126388946 1102 26-27 0.503611335848845 1102 28-29 0.41868061850483684 1102 30-31 0.4726003557581677 1102 32-33 0.6562067538975285 1102 34-35 0.5488889292199914 1102 36-37 0.5527626334067861 1102 38-39 0.5423153139909047 1102 40-41 0.6729186714884179 1102 42-43 0.8882569274408283 1102 44-45 0.9281953269730749 1102 46-47 1.1860856151016606 1102 48-49 1.302335241300014 1102 50-51 1.160710479204269 1102 52-53 0.8791132699110022 1102 54-55 1.1465536299199002 1102 56-57 1.1683672420644484 1102 58-59 1.3396887086877314 1102 60-61 1.2530797838657008 1102 62-63 1.1789767562672182 1102 64-65 1.2905891522771569 1102 66-67 1.2328949192645346 1102 68-69 1.4563615529053742 1102 70-71 1.6872953309315442 1102 72-73 1.8771737936505666 1102 74-75 1.9056855328986018 1102 76-77 2.1406599383808604 1102 78-79 2.203471170846811 1102 80-81 2.3902971906204478 1102 82-83 2.512609194455308 1102 84-85 2.76973550738413 1102 86-87 2.9156790117441034 1102 88-89 3.355541210829454 1102 90-91 3.3460668316310382 1102 92-93 3.7667017410132217 1102 94-95 4.137249671704087 1102 96-97 4.263368687779881 1102 98-99 4.4389910364491705 1102 100-101 4.017831482664496 1103 1 0.668353108535122 1103 2 0.5218457875164901 1103 3 0.48941556367593364 1103 4 0.38845359996344087 1103 5 0.35931458457380927 1103 6 0.38442299552622217 1103 7 0.3564324004168782 1103 8 0.49090789346643504 1103 9 0.49196527872766893 1103 10-11 0.6069002655528379 1103 12-13 0.6257030150049161 1103 14-15 0.8374338910223571 1103 16-17 0.9182999767756712 1103 18-19 0.840856581907083 1103 20-21 0.8204879533274152 1103 22-23 0.813727272238971 1103 24-25 0.781046802042269 1103 26-27 0.8297533460230184 1103 28-29 0.8487900724032471 1103 30-31 0.7953004109985855 1103 32-33 0.9140660968788126 1103 34-35 0.8811513500929564 1103 36-37 1.1090696063859582 1103 38-39 1.0490354490916616 1103 40-41 1.108112862048415 1103 42-43 1.1533498796890669 1103 44-45 1.0559101118743826 1103 46-47 1.023094483773761 1103 48-49 1.1149899967084451 1103 50-51 1.1818058773346891 1103 52-53 1.2943692250367178 1103 54-55 1.2719161661735257 1103 56-57 1.519081172517886 1103 58-59 1.647592989048654 1103 60-61 1.674123230492242 1103 62-63 1.8247859680608727 1103 64-65 1.9493661037978072 1103 66-67 2.0385301807772542 1103 68-69 2.115936570809854 1103 70-71 2.317025082430181 1103 72-73 2.235205905586593 1103 74-75 2.4991848157127876 1103 76-77 2.5443503074177656 1103 78-79 2.6856126735876877 1103 80-81 2.862871293668338 1103 82-83 3.0167109469124327 1103 84-85 3.1040618930428288 1103 86-87 3.222736898740859 1103 88-89 3.5806976190877258 1103 90-91 3.591541043481751 1103 92-93 3.9454280029011564 1103 94-95 4.349728973325522 1103 96-97 4.825866212700156 1103 98-99 5.2582135627286455 1103 100-101 5.000896567696488 1104 1 0.3231300147971794 1104 2 0.290079957872301 1104 3 0.3654866401713406 1104 4 0.20716567525259677 1104 5 0.3927636437654414 1104 6 0.2002774381133392 1104 7 0.22560278684327528 1104 8 0.3244169586586665 1104 9 0.2647990927213115 1104 10-11 0.31239173306693857 1104 12-13 0.3565776330789703 1104 14-15 0.5161730914730143 1104 16-17 0.5152840629393936 1104 18-19 0.5337033155600395 1104 20-21 0.5402124768001713 1104 22-23 0.4846702849075868 1104 24-25 0.5469205322724306 1104 26-27 0.47688744718823983 1104 28-29 0.5212335412399227 1104 30-31 0.4597223633979084 1104 32-33 0.5305245794617619 1104 34-35 0.48041505197334544 1104 36-37 0.5530913554996459 1104 38-39 0.6431135303691775 1104 40-41 0.7014277037366625 1104 42-43 0.7027146120806762 1104 44-45 0.7852643738311897 1104 46-47 0.8188931452725612 1104 48-49 0.9794030706689014 1104 50-51 0.9469353494655692 1104 52-53 1.1420574632482925 1104 54-55 1.2168484145003973 1104 56-57 1.2094483725031964 1104 58-59 1.2593869235441488 1104 60-61 1.1521613404256854 1104 62-63 1.3039657227710393 1104 64-65 1.3124621072757066 1104 66-67 1.3291701631428445 1104 68-69 1.5360180687555633 1104 70-71 1.8740574183168341 1104 72-73 2.0515532546072066 1104 74-75 2.2720409788801277 1104 76-77 2.2154898703536503 1104 78-79 2.4122583993847577 1104 80-81 2.4588897730672947 1104 82-83 2.7466113859359282 1104 84-85 2.7729983785281114 1104 86-87 3.0946712350316297 1104 88-89 3.2131611715654245 1104 90-91 3.1866875989536254 1104 92-93 3.5442664581752545 1104 94-95 3.891716335327029 1104 96-97 3.8065834680991664 1104 98-99 4.021157101980531 1104 100-101 4.118652526965883 1105 1 0.1823681100352772 1105 2 0.1375901619539306 1105 3 0.12725534765433366 1105 4 0.10048540314375742 1105 5 0.0783826913844905 1105 6 0.13208696192286595 1105 7 0.12737149432626893 1105 8 0.04603600627771698 1105 9 0.23503038523831776 1105 10-11 0.26199717524380617 1105 12-13 0.1298089255959738 1105 14-15 0.3030846560988607 1105 16-17 0.3003248792659221 1105 18-19 0.29620671692057954 1105 20-21 0.2987090754396249 1105 22-23 0.3049423937511264 1105 24-25 0.37011100846290645 1105 26-27 0.3405949301814317 1105 28-29 0.40048524192020096 1105 30-31 0.4276883497924686 1105 32-33 0.5434021304821712 1105 34-35 0.5198300179597339 1105 36-37 0.5522682262479464 1105 38-39 0.5455352990766613 1105 40-41 0.6247270234645583 1105 42-43 0.6640615508561822 1105 44-45 0.7598426051237084 1105 46-47 0.7426210364290284 1105 48-49 0.801960893798153 1105 50-51 0.8890714038873426 1105 52-53 0.7881595040646161 1105 54-55 0.9433314076976771 1105 56-57 1.035904154816123 1105 58-59 0.922536583408089 1105 60-61 1.2601409322624164 1105 62-63 1.1565099404581147 1105 64-65 1.2044825154389756 1105 66-67 1.4734422719863822 1105 68-69 1.572052082361001 1105 70-71 1.7789485747794238 1105 72-73 1.764505635559587 1105 74-75 2.0367824754787662 1105 76-77 2.078659938380863 1105 78-79 2.3277209844187716 1105 80-81 2.5566312696659352 1105 82-83 2.80111478729647 1105 84-85 2.764358922745796 1105 86-87 3.0188617112221046 1105 88-89 3.2591407634021614 1105 90-91 3.509551544531856 1105 92-93 3.507695029603827 1105 94-95 3.3718387843066218 1105 96-97 3.6066378898678764 1105 98-99 4.03767410878325 1105 100-101 3.8763736154012545 1106 1 0.11042314776274864 1106 2 0.1679869662149187 1106 3 0.02973204539068064 1106 4 0.17008948658805423 1106 5 0.1060728822411221 1106 6 -0.03206039715391995 1106 7 0.061841090420365674 1106 8 0.1923644572319958 1106 9 0.22647468173187946 1106 10-11 0.13935713973559416 1106 12-13 0.36234998196525936 1106 14-15 0.4016758678165715 1106 16-17 0.22635728050870796 1106 18-19 0.2390109779059344 1106 20-21 0.3067867496517849 1106 22-23 0.4040236187844144 1106 24-25 0.38971109723343744 1106 26-27 0.4393366079444192 1106 28-29 0.41488826011815405 1106 30-31 0.4020518651053848 1106 32-33 0.4673670661235363 1106 34-35 0.44645851329928377 1106 36-37 0.45589849699805995 1106 38-39 0.17192500169539215 1106 40-41 0.6121327047783609 1106 42-43 0.683621249036392 1106 44-45 0.5978865465351646 1106 46-47 0.5369565890255643 1106 48-49 0.6992383016987276 1106 50-51 0.6419981682015887 1106 52-53 0.8013539115213391 1106 54-55 0.34599141657472643 1106 56-57 0.7740927921885117 1106 58-59 0.8500700942824793 1106 60-61 1.158841775582431 1106 62-63 1.034106034554874 1106 64-65 1.044463429775412 1106 66-67 1.3161222542322726 1106 68-69 1.1331248741941131 1106 70-71 1.363533128707516 1106 72-73 1.699549576971041 1106 74-75 1.679843727143215 1106 76-77 1.8703536800586242 1106 78-79 2.1128177442944924 1106 80-81 2.1988718377973164 1106 82-83 2.2925759501903897 1106 84-85 2.552721106500794 1106 86-87 2.539215017924281 1106 88-89 3.0666236750754834 1106 90-91 3.131749058957066 1106 92-93 3.5092813589424843 1106 94-95 3.491114416796634 1106 96-97 3.560502958665033 1106 98-99 3.9157158309314966 1106 100-101 4.06198613204573 1107 1 0.23963469112214142 1107 2 0.06315113756368618 1107 3 0.03231311402146986 1107 4 -0.06843971452847342 1107 5 0.016848245513713778 1107 6 0.2149299229840551 1107 7 0.10659271811745441 1107 8 0.15179638282884866 1107 9 0.21919641005646184 1107 10-11 0.18243705543208222 1107 12-13 0.11634508306281788 1107 14-15 0.20804999627857512 1107 16-17 0.3366659147815412 1107 18-19 0.24464574131082628 1107 20-21 0.3637587117254597 1107 22-23 0.3410656286676854 1107 24-25 0.41230655831314067 1107 26-27 0.4461725938528005 1107 28-29 0.3930184040939295 1107 30-31 0.549848811923404 1107 32-33 0.6535206585095565 1107 34-35 0.5173165391407366 1107 36-37 0.560501431211577 1107 38-39 0.5550235318537204 1107 40-41 0.679872509129936 1107 42-43 0.626180506782589 1107 44-45 0.6228194985768525 1107 46-47 0.7135247591504665 1107 48-49 0.7609527637168512 1107 50-51 0.6204475271222591 1107 52-53 0.7430516735126247 1107 54-55 0.8515885750061969 1107 56-57 0.9239148522915173 1107 58-59 0.8779311063006858 1107 60-61 1.0724716982016531 1107 62-63 1.018724745764061 1107 64-65 1.1103802475741915 1107 66-67 1.2815004662525347 1107 68-69 1.4515184922882618 1107 70-71 1.6138869573211458 1107 72-73 1.6955993454440552 1107 74-75 1.917374259817663 1107 76-77 2.170505894735161 1107 78-79 2.269148883434603 1107 80-81 2.2551002469872863 1107 82-83 2.5004506880239 1107 84-85 2.6924590511155024 1107 86-87 2.7611911934642954 1107 88-89 3.186830536615684 1107 90-91 3.4445190242066523 1107 92-93 3.7872825349525634 1107 94-95 4.215489618709704 1107 96-97 4.473746148318881 1107 98-99 4.673900895261642 1107 100-101 4.166110457506518 1108 1 0.12477836644553264 1108 2 -0.030563684199911734 1108 3 0.05584509124407333 1108 4 -0.07364280198444817 1108 5 0.025639101640898332 1108 6 0.06126644910234802 1108 7 0.14352534048011734 1108 8 -0.03886142961972183 1108 9 0.05649192369985201 1108 10-11 0.02631768806432433 1108 12-13 0.002116617903674012 1108 14-15 0.13162311763732504 1108 16-17 0.3198889818300259 1108 18-19 0.05182210153596856 1108 20-21 0.04773471646526417 1108 22-23 -0.03569863188990752 1108 24-25 0.01253408538598677 1108 26-27 0.014184673771175937 1108 28-29 0.24343395986891636 1108 30-31 0.3198806574847808 1108 32-33 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-1.326849082787362 2316 66-67 -1.5668927964539527 2316 68-69 -1.649904154384707 2316 70-71 -1.786108105382521 2316 72-73 -1.5297847056781215 2316 74-75 -1.9397756506056751 2316 76-77 -2.0591665509193113 2316 78-79 -2.685780654293392 2316 80-81 -2.6032064014348926 2316 82-83 -2.706693579775056 2316 84-85 -2.497648017682195 2316 86-87 -2.349651109290715 2316 88-89 -2.8382544554141127 2316 90-91 -2.905048609699879 2316 92-93 -3.243857698369954 2316 94-95 -3.550136924195403 2316 96-97 -3.7212911635348647 2316 98-99 -3.91722043526919 2316 100-101 -3.457949692869418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4380.0 3 1427.0 4 1495.0 5 1613.0 6 1949.0 7 2432.0 8 2986.0 9 3764.0 10 4577.0 11 5522.0 12 6942.0 13 8302.0 14 9588.0 15 11332.0 16 13687.0 17 16558.0 18 16552.0 19 14274.0 20 14664.0 21 15957.0 22 17446.0 23 19478.0 24 22095.0 25 24867.0 26 27723.0 27 32133.0 28 36705.0 29 42446.0 30 49795.0 31 59238.0 32 71677.0 33 89140.0 34 115121.0 35 153765.0 36 198236.0 37 220183.0 38 113299.0 39 5778.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88048650850935 18.56242429757456 14.407606331531985 23.149482862384108 2 15.558289520477503 19.75642844098549 40.42492863390394 24.260353404633065 3 19.621350903742684 23.718061395726963 29.375894335850063 27.28469336468029 4 12.935578230358297 16.554170042709806 36.58966999464411 33.92058173228779 5 14.094861839253985 36.83329567175773 33.87398871801142 15.197853770976868 6 32.3124060759744 35.57615435546484 17.337104488847253 14.774335079713511 7 28.856016862457 32.49256774849123 20.175490391283155 18.475924997768608 8 26.185899240272036 35.14438675961537 19.396817463445043 19.27289653666755 9 26.298509143289493 17.142586431102977 20.164808531265148 36.394095894342385 10-11 25.376971627784823 26.544096316881717 26.69604189660766 21.382890158725797 12-13 26.10027463096464 23.750841057329215 27.475077239958807 22.67380707174734 14-15 22.81265318396081 25.31965244661818 25.89394211929023 25.973752250130786 16-17 22.368042554272417 28.651704292294244 26.48307310170968 22.497180051723653 18-19 22.183700568773222 27.008866655498093 28.981687204936872 21.825745570791806 20-21 22.815600146257808 26.158998717669213 29.155052160135135 21.870348975937844 22-23 22.592277518814598 26.01580968506278 29.1326713122121 22.259241483910525 24-25 22.44989196036063 26.216600506050597 29.213039472839352 22.12046806074942 26-27 22.176779892913732 26.571333908992123 28.9502352704881 22.301650927606044 28-29 22.021439184011996 26.79050073738819 29.113479511044343 22.07458056755547 30-31 22.251311327002163 26.370829784451104 29.037804049519224 22.34005483902751 32-33 22.05026184840428 26.490647780854083 28.98128869580442 22.47780167493722 34-35 22.59877986397921 26.644213966678 28.711647595604216 22.04535857373858 36-37 22.49271101495834 26.376908412997512 28.820130197596463 22.310250374447683 38-39 22.23965056665192 26.614397650674427 28.84592744782337 22.300024334850285 40-41 22.47448156563403 26.48765436336054 28.820077018650597 22.217787052354833 42-43 22.3344939961868 26.429202712141308 28.662034311916845 22.57426897975504 44-45 22.37332459344955 26.554260779142336 28.625407322626977 22.44700730478114 46-47 22.4792278185228 26.86915310456933 28.208155655442642 22.44346342146523 48-49 22.53496859322138 26.832313784282576 28.13207150194797 22.500646120548076 50-51 22.45041399177576 27.032568176685913 28.115165421682697 22.401852409855632 52-53 22.749190237688147 26.95563003591851 27.86141756279905 22.433762163594295 54-55 22.34850954796703 26.883150400128493 28.05505087146949 22.71328918043499 56-57 22.445792117519595 27.13512014603465 28.014101033382538 22.40498670306322 58-59 22.58290466354069 26.81021971207255 28.011925079422166 22.594950544964597 60-61 22.5219734019734 26.799725439725442 28.334139854139856 22.344161304161304 62-63 22.389215798653176 26.938813611557073 28.2417433072347 22.430227282555055 64-65 22.17369847292615 26.88015133461093 28.121413627802518 22.8247365646604 66-67 22.218523592377743 27.01435007371331 27.944997789973343 22.822128543935605 68-69 22.247255560604167 26.840219198240707 28.065144487166993 22.847380753988137 70-71 22.261919593260966 26.881663967359838 27.97140828311387 22.885008156265325 72-73 22.35858617578389 26.780930965405314 27.961051160163237 22.89943169864756 74-75 22.271950108756158 27.082479654121787 27.69249565352876 22.953074583593295 76-77 22.366045935736093 26.843592826865216 27.766967835101358 23.023393402297334 78-79 22.419065124716617 26.996320377667487 27.710384002668604 22.874230494947298 80-81 22.35870363894135 27.113684013278938 27.632870899623068 22.894741448156644 82-83 22.468393418926233 26.992787257912244 27.667559751272446 22.871259571889077 84-85 22.510772445053323 26.916904948529748 27.583190455730367 22.98913215068657 86-87 22.51540502246455 26.81192815599213 27.65519378397864 23.01747303756468 88-89 22.48926013336793 26.781225244075397 27.563186386970195 23.166328235586477 90-91 22.520763918613195 26.87918740884625 27.49923026676877 23.10081840577178 92-93 22.690344598471807 26.484915751700207 27.616680531874877 23.208059117953113 94-95 22.528182797389356 26.505525345467102 27.580525465605692 23.38576639153785 96-97 22.62106574305152 26.34918597319562 27.56601300369764 23.46373528005522 98-99 22.57771779757643 26.369307038757476 27.301192976001133 23.75178218766496 100-101 23.306305145321616 25.42869661589216 27.43644595651091 23.828552282275318 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1124.0 1 747.0 2 510.5 3 636.5 4 482.5 5 418.5 6 808.5 7 1748.5 8 2734.0 9 3074.5 10 2464.5 11 1756.0 12 1646.5 13 1673.5 14 1755.0 15 1853.0 16 1887.0 17 1990.0 18 2128.0 19 2276.5 20 2531.0 21 2890.5 22 3414.5 23 4088.0 24 5041.5 25 6124.5 26 7472.0 27 9448.5 28 11206.0 29 13115.0 30 15335.0 31 17311.0 32 19751.0 33 22115.5 34 24328.0 35 26666.0 36 29682.5 37 32805.0 38 35594.5 39 37899.0 40 39625.5 41 42605.5 42 45763.0 43 48698.0 44 51412.5 45 53794.0 46 55363.5 47 56793.5 48 59774.0 49 60499.0 50 58769.0 51 57057.0 52 54506.5 53 52586.5 54 50867.5 55 47024.5 56 42113.5 57 37993.0 58 34378.5 59 30094.5 60 25325.0 61 20584.5 62 16359.0 63 12891.5 64 10226.5 65 8080.5 66 6164.0 67 4858.5 68 3842.5 69 2820.0 70 2094.5 71 1576.0 72 1231.5 73 929.5 74 737.5 75 637.5 76 432.0 77 249.0 78 139.0 79 86.0 80 56.0 81 35.5 82 23.0 83 10.5 84 6.5 85 7.5 86 5.0 87 3.0 88 5.0 89 4.5 90 4.0 91 3.5 92 2.5 93 2.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44917186296861084 2 0.03788279119307458 3 0.05064764474726276 4 0.053941800503182286 5 0.08077544426494346 6 0.0797460205912186 7 0.04364756376593376 8 0.03788279119307458 9 0.04447110270491365 10-11 0.04690740539939579 12-13 0.04296128131678386 14-15 0.037333765233754666 16-17 0.03699062400917971 18-19 0.04515738515406355 20-21 0.05445651234004472 22-23 0.04646132180744836 24-25 0.0650252620569532 26-27 0.056515359687494424 28-29 0.043510307276103784 30-31 0.046770148909565816 32-33 0.04484855805194609 34-35 0.050167247032857826 36-37 0.04443678858245615 38-39 0.025220880006258897 40-41 0.023059090291436705 42-43 0.02357380212829913 44-45 0.013965847840200505 46-47 0.02539245061854637 48-49 0.024328712822364023 50-51 0.014617816166892911 52-53 0.014892329146552872 54-55 0.015029585636382854 56-57 0.014514873799520425 58-59 0.013485450125795571 60-61 0.01722568947366254 62-63 0.014892329146552872 64-65 0.015235470371127824 66-67 0.008921671838948726 68-69 0.012353084084698235 70-71 0.01856394024950485 72-73 0.01650509290205514 74-75 0.013519764248253067 76-77 0.009196184818608686 78-79 0.012558968819443206 80-81 0.01595606694273522 82-83 0.01307368065630563 84-85 0.011529545145718353 86-87 0.0036029828580369854 88-89 0.027725810945656036 90-91 0.031054280824033064 92-93 0.016608035269427626 94-95 0.032598416334620336 96-97 0.02652481665964371 98-99 0.023951257475331576 100-101 0.1484772078735813 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1457126.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.67696238131045 #Duplication Level Percentage of deduplicated Percentage of total 1 82.77830966203786 53.538496200000885 2 10.427173353015306 13.487957973927472 3 2.875529853901497 5.579416085613667 4 1.279652457810312 3.31056135499796 5 0.6613953825446274 2.1388522138005643 6 0.4175122080913253 1.6202052825876299 7 0.2792891240648451 1.2644500519455792 8 0.21226053581270432 1.098269335183606 9 0.14305400180544312 0.8327068463939904 >10 0.8412223699121341 9.827363330249037 >50 0.05693229516358205 2.478550223404442 >100 0.025966120762739076 3.0665992757720018 >500 0.0010641469234859147 0.4339466093451527 >1k 4.256587693943659E-4 0.4786145474046299 >5k 2.1282938469718295E-4 0.8440106693734508 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6693 0.45932884321602935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5570 0.38225932417649533 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3487 0.23930669001857083 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.862824491499019E-5 0.0 5 0.0 0.0 0.0 6.862824491499019E-5 0.0 6 6.862824491499019E-5 0.0 0.0 6.862824491499019E-5 6.862824491499019E-5 7 6.862824491499019E-5 0.0 0.0 6.862824491499019E-5 6.862824491499019E-5 8 6.862824491499019E-5 0.0 0.0 6.862824491499019E-5 6.862824491499019E-5 9 6.862824491499019E-5 0.0 0.0 6.862824491499019E-5 6.862824491499019E-5 10-11 6.862824491499019E-5 0.0 0.0 6.862824491499019E-5 6.862824491499019E-5 12-13 6.862824491499019E-5 0.0 0.0 6.862824491499019E-5 6.862824491499019E-5 14-15 6.862824491499019E-5 0.0 0.0 6.862824491499019E-5 6.862824491499019E-5 16-17 6.862824491499019E-5 0.0 0.0 6.862824491499019E-5 6.862824491499019E-5 18-19 6.862824491499019E-5 0.0 0.0 6.862824491499019E-5 6.862824491499019E-5 20-21 6.862824491499019E-5 0.0 0.0 1.7157061228747548E-4 6.862824491499019E-5 22-23 6.862824491499019E-5 0.0 0.0 3.7745534703244603E-4 1.0294236737248529E-4 24-25 6.862824491499019E-5 0.0 0.0 4.8039771440493135E-4 1.3725648982998038E-4 26-27 6.862824491499019E-5 0.0 0.0 0.0012009942860123283 1.3725648982998038E-4 28-29 6.862824491499019E-5 0.0 0.0 0.0033284698783770245 1.3725648982998038E-4 30-31 6.862824491499019E-5 0.0 0.0 0.009367755430896161 1.3725648982998038E-4 32-33 6.862824491499019E-5 0.0 0.0 0.022578692577031774 1.3725648982998038E-4 34-35 6.862824491499019E-5 0.0 0.0 0.043784820255763746 1.3725648982998038E-4 36-37 6.862824491499019E-5 0.0 0.0 0.08159898320392334 1.3725648982998038E-4 38-39 6.862824491499019E-5 0.0 0.0 0.13883493946302516 1.3725648982998038E-4 40-41 6.862824491499019E-5 0.0 0.0 0.19857582666152412 1.3725648982998038E-4 42-43 6.862824491499019E-5 0.0 0.0 0.2575961172884157 1.3725648982998038E-4 44-45 6.862824491499019E-5 0.0 0.0 0.33058225575550776 1.3725648982998038E-4 46-47 6.862824491499019E-5 0.0 0.0 0.41798032565474774 2.0588473474497058E-4 48-49 6.862824491499019E-5 0.0 0.0 0.5082607818404173 2.0588473474497058E-4 50-51 6.862824491499019E-5 0.0 0.0 0.5990559498629494 2.0588473474497058E-4 52-53 6.862824491499019E-5 0.0 0.0 0.6872775587011692 2.0588473474497058E-4 54-55 6.862824491499019E-5 0.0 0.0 0.7834257298270706 2.0588473474497058E-4 56-57 6.862824491499019E-5 0.0 0.0 0.8945005442219822 2.0588473474497058E-4 58-59 6.862824491499019E-5 0.0 0.0 1.0166931342931222 2.0588473474497058E-4 60-61 6.862824491499019E-5 0.0 0.0 1.1502780130201506 2.0588473474497058E-4 62-63 1.3725648982998038E-4 0.0 0.0 1.2748725916633155 2.0588473474497058E-4 64-65 1.3725648982998038E-4 0.0 0.0 1.4059525394509467 2.0588473474497058E-4 66-67 1.3725648982998038E-4 0.0 0.0 1.5571748771211276 2.0588473474497058E-4 68-69 1.3725648982998038E-4 0.0 0.0 1.725966045489546 2.0588473474497058E-4 70-71 1.3725648982998038E-4 0.0 0.0 1.9075220674121525 2.0588473474497058E-4 72-73 1.3725648982998038E-4 0.0 0.0 2.0839995992110496 2.7451297965996077E-4 74-75 1.3725648982998038E-4 0.0 0.0 2.2587957390095297 2.7451297965996077E-4 76-77 1.3725648982998038E-4 0.0 0.0 2.4462537899948256 2.7451297965996077E-4 78-79 1.3725648982998038E-4 0.0 0.0 2.6592415480884974 2.7451297965996077E-4 80-81 1.3725648982998038E-4 0.0 0.0 2.889043226186342 2.7451297965996077E-4 82-83 1.3725648982998038E-4 0.0 0.0 3.1169233134265673 2.7451297965996077E-4 84-85 1.3725648982998038E-4 0.0 0.0 3.345421054871027 2.7451297965996077E-4 86-87 1.3725648982998038E-4 3.4314122457495096E-5 0.0 3.5798551395006335 2.7451297965996077E-4 88-89 1.3725648982998038E-4 6.862824491499019E-5 0.0 3.83237276666534 2.7451297965996077E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4205 0.0 39.710533 1 GTATCAA 6350 0.0 34.485985 1 ATCAACG 7755 0.0 27.574053 3 TATCAAC 7860 0.0 27.386131 2 TCAACGC 7895 0.0 27.26566 4 CAACGCA 7985 0.0 26.95834 5 AACGCAG 8140 0.0 26.441376 6 GTACATG 5165 0.0 24.570557 1 TACATGG 5135 0.0 24.059572 2 ACGCAGA 9000 0.0 23.59233 7 CGCAGAG 9130 0.0 23.152351 8 GAGTACT 5135 0.0 22.84953 12-13 ACATGGG 5270 0.0 22.812864 3 GTACCTG 1030 0.0 21.770296 1 GCAGAGT 9625 0.0 21.51749 9 GTACTTT 5500 0.0 21.463074 14-15 AGAGTAC 7910 0.0 20.778082 10-11 AGTACTT 5510 0.0 19.613295 12-13 TACCTGG 1050 0.0 19.45961 2 CAGAGTA 9345 0.0 19.442762 10-11 >>END_MODULE