FastQCFastQC Report
Thu 26 May 2016
SRR938198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457126
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64230.440799217088982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57940.3976320510374532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35800.24568911679566488No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14700.10088352002503559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41000.039.2726551
GTATCAA61900.033.148781
ATCAACG74700.026.9599913
TCAACGC76900.026.2486694
TATCAAC78000.026.0020562
CAACGCA77300.025.9899565
AACGCAG79300.025.334476
GTACATG54000.023.045281
TACATGG54200.022.6097052
ACGCAGA90000.022.269717
TATACGT1302.9442756E-421.9206055
CGCAGAG91500.021.8008218
ACATGGG54300.021.6058623
GAGTACT54350.021.06018412-13
GCAGAGT96950.020.2813669
GTACTTT56300.020.20420614-15
AGTACTT55350.020.03613312-13
AGAGTAC84300.018.98659110-11
GTGGTAT25100.018.7342341
TGGTATC23650.018.6778242
Produced by FastQC (version 0.11.5)