FastQCFastQC Report
Thu 26 May 2016
SRR938197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457012
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68760.47192473363294196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56160.38544637930229814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34050.23369745753638269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41850.040.0295221
GTATCAA62900.031.9448031
ATCAACG74750.026.6325443
GACCGTA908.89716E-426.4150077
TATCAAC75800.026.2636242
TCAACGC76000.026.2570254
CAACGCA76650.025.910395
AACGCAG79550.025.085286
GTACATG53750.024.685111
TACATGG52700.024.0719052
TATATCG1000.00165128323.7563555
GAGTACT49800.023.10279312-13
ACGCAGA88500.022.242337
CGCAGAG88400.022.2137098
ACATGGG54000.022.17263
GTACTTT53850.021.04070514-15
AGTACTT51950.020.95696412-13
GCAGAGT95300.020.1556479
CATGGGG33550.019.9680844
AGAGTAC79700.019.17922210-11
Produced by FastQC (version 0.11.5)