##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938197_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1457012 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.862675118667518 33.0 31.0 34.0 30.0 34.0 2 32.05041825324706 34.0 31.0 34.0 30.0 34.0 3 32.124472550672195 34.0 31.0 34.0 30.0 34.0 4 35.640906869675746 37.0 35.0 37.0 33.0 37.0 5 35.657389918545626 37.0 35.0 37.0 35.0 37.0 6 35.684407540912495 37.0 35.0 37.0 35.0 37.0 7 35.625044268681386 37.0 35.0 37.0 33.0 37.0 8 35.60798881546617 37.0 35.0 37.0 33.0 37.0 9 37.269235943149404 39.0 37.0 39.0 34.0 39.0 10-11 37.181900011804984 39.0 37.0 39.0 34.0 39.0 12-13 37.078345614174765 39.0 37.0 39.0 33.0 39.0 14-15 38.324663420754256 40.0 38.0 41.0 33.5 41.0 16-17 38.24165621147938 40.0 38.0 41.0 33.0 41.0 18-19 38.16999928621041 40.0 38.0 41.0 33.0 41.0 20-21 38.06217073023421 40.0 38.0 41.0 33.0 41.0 22-23 37.90160925236031 40.0 38.0 41.0 33.0 41.0 24-25 37.851442541310576 40.0 38.0 41.0 33.0 41.0 26-27 37.8755665704881 40.0 38.0 41.0 33.0 41.0 28-29 37.782528215278944 40.0 38.0 41.0 33.0 41.0 30-31 37.60477538963303 40.0 37.5 41.0 32.0 41.0 32-33 37.3947647651495 40.0 37.0 41.0 32.0 41.0 34-35 37.18624211742937 40.0 37.0 41.0 31.0 41.0 36-37 36.977723930894186 40.0 36.0 41.0 31.0 41.0 38-39 36.747298924099454 39.5 36.0 41.0 30.0 41.0 40-41 36.527634638561665 39.0 35.5 41.0 30.0 41.0 42-43 36.25551882894581 39.0 35.0 41.0 30.0 41.0 44-45 36.02978287069701 39.0 35.0 41.0 29.0 41.0 46-47 35.880684579124946 39.0 35.0 41.0 28.0 41.0 48-49 35.64190274342284 39.0 35.0 41.0 27.0 41.0 50-51 35.022488833310916 38.5 34.5 40.5 26.5 40.5 52-53 35.1130944700524 38.5 35.0 40.0 26.5 41.0 54-55 35.256982440776056 39.0 35.0 40.0 26.0 41.0 56-57 35.04551541099181 39.0 35.0 40.0 26.0 41.0 58-59 34.785576920437165 38.0 34.5 40.0 25.5 41.0 60-61 34.419216863004564 38.0 34.0 40.0 24.5 41.0 62-63 33.995530235852556 37.5 34.0 40.0 23.0 41.0 64-65 33.5587013010188 37.0 33.0 40.0 22.0 41.0 66-67 33.11554263108334 36.0 33.0 39.0 20.5 41.0 68-69 32.621587536684665 36.0 32.0 39.0 18.5 40.5 70-71 32.13189905093438 35.0 32.0 38.5 17.5 40.0 72-73 31.624429654663103 35.0 31.0 37.5 12.0 40.0 74-75 31.08024333361702 35.0 31.0 37.0 7.5 39.0 76-77 30.5191755455686 35.0 30.0 36.5 6.0 39.0 78-79 29.93557362602367 34.0 29.5 36.0 2.0 38.5 80-81 29.380831111892007 34.0 29.0 35.5 2.0 37.0 82-83 28.82721315953472 34.0 29.0 35.0 2.0 37.0 84-85 28.317011802236358 34.0 27.5 35.0 2.0 36.0 86-87 27.806106607220805 33.5 26.5 35.0 2.0 36.0 88-89 27.23326232042015 33.0 26.0 35.0 2.0 35.5 90-91 26.607536863114376 33.0 25.0 35.0 2.0 35.0 92-93 25.963137915130417 32.0 23.5 35.0 2.0 35.0 94-95 25.333024710846583 32.0 20.0 34.0 2.0 35.0 96-97 24.471613480190967 31.5 12.5 34.0 2.0 35.0 98-99 23.375565540983878 31.0 2.0 34.0 2.0 35.0 100-101 20.483023132273445 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3391718172552096 1101 2 0.3393106069975431 1101 3 0.40072453870018165 1101 4 0.33582942987744957 1101 5 0.27521907876692353 1101 6 0.24370644319556334 1101 7 0.2993404961596582 1101 8 0.21131232573523562 1101 9 0.21906410945620536 1101 10-11 0.3682159088068673 1101 12-13 0.2384945013251496 1101 14-15 0.45761286762051867 1101 16-17 0.46639362657521843 1101 18-19 0.3980246759345718 1101 20-21 0.3972258264835986 1101 22-23 0.42841860878062477 1101 24-25 0.5131572367925941 1101 26-27 0.4029780987359928 1101 28-29 0.43834908694199015 1101 30-31 0.5839152719543677 1101 32-33 0.521427060989403 1101 34-35 0.5824635264169586 1101 36-37 0.5396297530883061 1101 38-39 0.41398702137350085 1101 40-41 0.5156413015557462 1101 42-43 0.4974950617735985 1101 44-45 0.5876618237974895 1101 46-47 0.49463441420925847 1101 48-49 0.5930478245867974 1101 50-51 0.7190227793707251 1101 52-53 0.7258884731261475 1101 54-55 0.7187656787445391 1101 56-57 0.6538277940646324 1101 58-59 0.7247856802018973 1101 60-61 0.8722985249051192 1101 62-63 0.9054640689559861 1101 64-65 0.7810161834083331 1101 66-67 0.5701551271179994 1101 68-69 0.7705643299549578 1101 70-71 0.8105968978058797 1101 72-73 0.8594810266480266 1101 74-75 0.8882434674460207 1101 76-77 0.7866293226705459 1101 78-79 0.8654292172793063 1101 80-81 0.9412248382843451 1101 82-83 0.7761664121228335 1101 84-85 0.8647660009218399 1101 86-87 0.8424911089911582 1101 88-89 0.8752764336333172 1101 90-91 0.9071597333407269 1101 92-93 1.0090496082988132 1101 94-95 1.1334695423795402 1101 96-97 1.1542280840760277 1101 98-99 1.660800359967464 1101 100-101 1.4941431161120775 1102 1 0.4881626492201825 1102 2 0.40103063983826814 1102 3 0.3641481840203795 1102 4 0.32620128264154147 1102 5 0.3406969796974124 1102 6 0.4187372313729014 1102 7 0.31214666747875697 1102 8 0.35043212595417117 1102 9 0.5271494269165231 1102 10-11 0.5174727164149644 1102 12-13 0.37991315211879595 1102 14-15 0.35188712186243976 1102 16-17 0.3978394735921853 1102 18-19 0.44015487571563483 1102 20-21 0.4102586329970137 1102 22-23 0.4102612811944226 1102 24-25 0.4859310805254893 1102 26-27 0.41538402374967376 1102 28-29 0.4486075365862163 1102 30-31 0.4258530456270577 1102 32-33 0.5009007542023269 1102 34-35 0.6533379106534127 1102 36-37 0.7977407273630703 1102 38-39 0.5962222430483877 1102 40-41 0.6366687714517454 1102 42-43 0.7467638425617764 1102 44-45 0.5949919756858293 1102 46-47 0.5983820195732434 1102 48-49 0.4196419679912893 1102 50-51 0.5238171827642617 1102 52-53 0.6049679066236777 1102 54-55 0.5494332690565287 1102 56-57 0.5069365447488181 1102 58-59 0.4490031473611751 1102 60-61 0.5602039025734271 1102 62-63 0.46113593540371056 1102 64-65 0.4636225736655817 1102 66-67 0.4995569812504499 1102 68-69 0.7883120037371185 1102 70-71 0.7608064284572151 1102 72-73 0.7905103780437628 1102 74-75 0.9744460878620025 1102 76-77 0.7062704693591044 1102 78-79 0.6428076039788131 1102 80-81 0.6289446316614651 1102 82-83 0.7406565256969984 1102 84-85 0.7868205986229881 1102 86-87 0.7830759133151872 1102 88-89 0.9017670945528593 1102 90-91 1.0215010373910829 1102 92-93 0.9854265240076252 1102 94-95 0.9571276909838105 1102 96-97 1.0428509973217857 1102 98-99 1.4496353695460087 1102 100-101 1.0979343380606252 1103 1 0.2531122829198189 1103 2 0.25748668379431194 1103 3 0.20911155398375314 1103 4 0.07655613612139689 1103 5 0.10824505662048978 1103 6 0.13634254272820812 1103 7 0.2857158066728971 1103 8 0.34405621020325583 1103 9 0.39806288845498017 1103 10-11 0.2839962970010461 1103 12-13 0.31602807885872153 1103 14-15 0.43941573907856224 1103 16-17 0.39658603952624816 1103 18-19 0.33194745813321447 1103 20-21 0.435001078051954 1103 22-23 0.4876708507914955 1103 24-25 0.6767335072654177 1103 26-27 0.277758107998757 1103 28-29 0.5647648168059973 1103 30-31 0.5599247031362111 1103 32-33 0.5869527230667941 1103 34-35 0.6314972513127515 1103 36-37 0.5059595918319388 1103 38-39 0.4142396423157777 1103 40-41 0.5545659784114534 1103 42-43 0.5349334854189252 1103 44-45 0.5853250800814322 1103 46-47 0.8450280818443048 1103 48-49 0.598533909383228 1103 50-51 0.6073222193943053 1103 52-53 0.599439060469841 1103 54-55 0.5353249815107333 1103 56-57 0.5789088432836209 1103 58-59 0.6622872590827882 1103 60-61 0.3895651663096942 1103 62-63 0.257335569103347 1103 64-65 0.2610928117608111 1103 66-67 0.27679484755081774 1103 68-69 0.3854731758982908 1103 70-71 0.4178176464059362 1103 72-73 0.6804485648569454 1103 74-75 0.6486619761403922 1103 76-77 0.6158744070880431 1103 78-79 0.5023371369458509 1103 80-81 0.7330483404526724 1103 82-83 0.6348586777116481 1103 84-85 0.6709552140076056 1103 86-87 0.740373303425077 1103 88-89 0.755218330816593 1103 90-91 0.8555955886731326 1103 92-93 0.8748152602713617 1103 94-95 1.0960184876871075 1103 96-97 1.1288228379811258 1103 98-99 0.9698105069086473 1103 100-101 0.7947578270716136 1104 1 0.44751492461309894 1104 2 0.37046237805079585 1104 3 0.30224928653034766 1104 4 0.4084387034719441 1104 5 0.44689786522403807 1104 6 0.3849215499279097 1104 7 0.22304055089418995 1104 8 0.37723616653592273 1104 9 0.29134497582573715 1104 10-11 0.5190799325772915 1104 12-13 0.31452859312278747 1104 14-15 0.4625467952145428 1104 16-17 0.632340925034832 1104 18-19 0.6720961436020971 1104 20-21 0.5415060673155665 1104 22-23 0.5650360143635496 1104 24-25 0.6888160060474249 1104 26-27 0.7072082965622499 1104 28-29 0.7705877295254595 1104 30-31 0.7474777084232116 1104 32-33 0.7608281087648407 1104 34-35 0.7855364797362157 1104 36-37 0.732271867405295 1104 38-39 0.636342170329641 1104 40-41 0.8478408480410025 1104 42-43 0.8974481464557513 1104 44-45 0.6944176295680649 1104 46-47 0.7871905171101901 1104 48-49 0.863710112490466 1104 50-51 0.9327611482815072 1104 52-53 0.9829844149511544 1104 54-55 0.9522397580314319 1104 56-57 0.7826025223467568 1104 58-59 0.8504718167254737 1104 60-61 0.9222398807578074 1104 62-63 1.0201172865648616 1104 64-65 0.7304571092039325 1104 66-67 0.8728214810158761 1104 68-69 1.017581297661586 1104 70-71 0.8797422570990108 1104 72-73 1.0565019039644739 1104 74-75 0.9203413640759344 1104 76-77 1.1728909537597616 1104 78-79 1.0250116709504304 1104 80-81 1.2345315862708972 1104 82-83 1.1924382081975864 1104 84-85 1.1874260994440107 1104 86-87 1.386247462107118 1104 88-89 1.4305192204031982 1104 90-91 1.5771964836730454 1104 92-93 1.4677563093700456 1104 94-95 1.5979781044250494 1104 96-97 1.4059444027874193 1104 98-99 1.6929920872330833 1104 100-101 1.450574424267522 1105 1 0.5623609290105698 1105 2 0.4568662515949313 1105 3 0.47542068345077837 1105 4 0.32505851093956295 1105 5 0.3354459259266207 1105 6 0.34560696251894996 1105 7 0.42576523093284635 1105 8 0.37140430070924424 1105 9 0.3730247686800183 1105 10-11 0.34925209383578704 1105 12-13 0.2686228525061267 1105 14-15 0.3916687492263051 1105 16-17 0.4476034431752325 1105 18-19 0.4426718214891565 1105 20-21 0.4154189058382016 1105 22-23 0.3701129700757164 1105 24-25 0.44094823990119636 1105 26-27 0.3432470050666012 1105 28-29 0.49723585454019315 1105 30-31 0.4156304009471725 1105 32-33 0.5834562195702873 1105 34-35 0.5991541558117675 1105 36-37 0.5799732974245941 1105 38-39 0.5262960070037224 1105 40-41 0.5983467590343423 1105 42-43 0.5921674794950249 1105 44-45 0.7124856388209366 1105 46-47 0.8325861378234194 1105 48-49 0.7797894959019729 1105 50-51 0.7624215060725419 1105 52-53 0.757782471444834 1105 54-55 0.7591888369535411 1105 56-57 0.9896472277062074 1105 58-59 0.8873166156910912 1105 60-61 0.964584883434668 1105 62-63 0.8260966326012493 1105 64-65 0.9139066645746752 1105 66-67 1.0414354700861779 1105 68-69 1.0347519672823466 1105 70-71 1.0650774041920101 1105 72-73 1.1131386817575901 1105 74-75 1.0815729248408168 1105 76-77 1.109063673961522 1105 78-79 1.1442618788705907 1105 80-81 1.0166699819690521 1105 82-83 1.0633862904497917 1105 84-85 1.1066756339385506 1105 86-87 1.2605391025382424 1105 88-89 1.2840491491210955 1105 90-91 1.011057670451006 1105 92-93 1.3151749006310034 1105 94-95 1.1343801260487467 1105 96-97 1.2011960787754177 1105 98-99 1.0558472771559444 1105 100-101 1.0281665236382054 1106 1 0.17255265181522716 1106 2 0.12400164022752591 1106 3 0.12454457690996179 1106 4 0.29376526668202274 1106 5 0.054761709812893855 1106 6 0.18333029611923735 1106 7 0.27838880125325005 1106 8 0.1608905566016361 1106 9 0.1902418266829642 1106 10-11 -0.3750509228091161 1106 12-13 -0.16623604666153113 1106 14-15 -0.06552257873442358 1106 16-17 0.10903850531659742 1106 18-19 0.23752455587652577 1106 20-21 0.17975085597611695 1106 22-23 0.060217287098147665 1106 24-25 0.16944018750615442 1106 26-27 0.16977735124415716 1106 28-29 0.10892546995847852 1106 30-31 0.28361121190446426 1106 32-33 0.3337525853298686 1106 34-35 0.2142160084865523 1106 36-37 0.04461451093644797 1106 38-39 0.23880771729257333 1106 40-41 0.3777898707770362 1106 42-43 0.3258994903495065 1106 44-45 0.49119936020639443 1106 46-47 0.7193374010446334 1106 48-49 0.7362940375384497 1106 50-51 0.7040611979452649 1106 52-53 0.6926702389189998 1106 54-55 0.32098936254296717 1106 56-57 0.27339986543131545 1106 58-59 0.4117794789428544 1106 60-61 0.2598012653605082 1106 62-63 0.40282044299421216 1106 64-65 0.42882669005327756 1106 66-67 0.33396989321360593 1106 68-69 0.024817777708832978 1106 70-71 0.3707136151057142 1106 72-73 0.5078594600471362 1106 74-75 0.7279098626771052 1106 76-77 0.452363712514682 1106 78-79 0.5299708565414214 1106 80-81 0.5572758159744318 1106 82-83 0.3818968348315508 1106 84-85 0.570170961929076 1106 86-87 0.6932438207370133 1106 88-89 0.5538092721187056 1106 90-91 0.32678661381511276 1106 92-93 0.44093572019291116 1106 94-95 0.5128613178814376 1106 96-97 0.49203984350058505 1106 98-99 0.7354905580110405 1106 100-101 0.05767365783485445 1107 1 0.19606899790542442 1107 2 0.17207111446541745 1107 3 0.20619317667272696 1107 4 0.22770999430085936 1107 5 0.19392711367483884 1107 6 0.2946117193821536 1107 7 0.2083107429250859 1107 8 0.27724138790583197 1107 9 0.23060565757030815 1107 10-11 0.29815429920894587 1107 12-13 0.0959541934532524 1107 14-15 0.20592847781717438 1107 16-17 0.334235127142513 1107 18-19 0.4680334557425212 1107 20-21 0.5092939115043436 1107 22-23 0.6037818135866289 1107 24-25 0.5726712530390117 1107 26-27 0.5774286060277589 1107 28-29 0.44078428868889574 1107 30-31 0.5580138322168082 1107 32-33 0.5458467509036993 1107 34-35 0.6470396428236285 1107 36-37 0.6427570250837391 1107 38-39 0.5756107072324781 1107 40-41 0.743566625389775 1107 42-43 0.8731412833115471 1107 44-45 0.7708053472425931 1107 46-47 0.8870905717728519 1107 48-49 0.8045984292363215 1107 50-51 0.7072178370180922 1107 52-53 0.7452958738250075 1107 54-55 0.860750267540098 1107 56-57 0.9178064307038554 1107 58-59 0.7720426820358455 1107 60-61 0.816542719288563 1107 62-63 0.9755166220184748 1107 64-65 0.8895016539582414 1107 66-67 1.018314260508177 1107 68-69 0.9704048343033591 1107 70-71 1.0053843585294402 1107 72-73 1.037893265046236 1107 74-75 0.9222633364784549 1107 76-77 1.077595177016299 1107 78-79 1.0448852213849378 1107 80-81 0.9597509877725265 1107 82-83 1.0173467302587973 1107 84-85 0.9818783246024196 1107 86-87 1.0694861253809727 1107 88-89 1.284767682755966 1107 90-91 1.340920484831912 1107 92-93 1.3623860611054575 1107 94-95 1.3151579824667863 1107 96-97 1.2730961177764044 1107 98-99 1.3096857771844448 1107 100-101 1.415098681227306 1108 1 0.2906978648771279 1108 2 0.20268546407162802 1108 3 0.17233192872244985 1108 4 0.21432534771259526 1108 5 0.21268572938576824 1108 6 0.23376933060002614 1108 7 0.17149050318057846 1108 8 0.20946431867419335 1108 9 0.296958233947187 1108 10-11 0.2359675587236154 1108 12-13 0.1842987479801792 1108 14-15 0.32685245158071297 1108 16-17 0.3414691014904676 1108 18-19 0.2793665747931726 1108 20-21 0.4808710893669925 1108 22-23 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0.8930819942038184 1108 90-91 0.920986436917385 1108 92-93 0.9987609480913946 1108 94-95 1.0813271580743127 1108 96-97 1.0143259256438988 1108 98-99 1.3525176775739318 1108 100-101 1.2304663593770044 1109 1 0.29025018028338323 1109 2 0.22682904805910198 1109 3 0.22490948430918678 1109 4 0.20548611540398554 1109 5 0.17070676040800237 1109 6 0.3159872985365766 1109 7 0.22205393962597952 1109 8 0.12691035652707683 1109 9 0.24250871061589407 1109 10-11 0.28789204864009577 1109 12-13 0.2812150780372633 1109 14-15 0.3722130545980207 1109 16-17 0.2706781674265599 1109 18-19 0.3854825924864045 1109 20-21 0.19831472726123422 1109 22-23 0.40420448171158085 1109 24-25 0.3765366660248475 1109 26-27 0.4708077006252225 1109 28-29 0.3924099032663122 1109 30-31 0.4489431834805018 1109 32-33 0.47068077216861326 1109 34-35 0.3800447076176141 1109 36-37 0.3011723715299084 1109 38-39 0.29766080507403103 1109 40-41 0.338472011259654 1109 42-43 0.26243029514422034 1109 44-45 0.45447034478382164 1109 46-47 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-1.1158956467596894 2316 60-61 -0.7448192531811131 2316 62-63 -0.8508403646045011 2316 64-65 -0.8258153730197009 2316 66-67 -1.121579233801988 2316 68-69 -1.1180963042669347 2316 70-71 -1.079372014613515 2316 72-73 -1.2365187882806161 2316 74-75 -1.2260700390171557 2316 76-77 -1.1946522523747873 2316 78-79 -1.037459217744125 2316 80-81 -1.021549160953338 2316 82-83 -1.142446918742884 2316 84-85 -1.1106360335410876 2316 86-87 -1.23605603683405 2316 88-89 -1.1801061683376943 2316 90-91 -1.2682678585945695 2316 92-93 -1.4179752154978367 2316 94-95 -1.4067391916663148 2316 96-97 -1.4512379101647284 2316 98-99 -1.2875444632122814 2316 100-101 -1.3937602678294603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4209.0 3 1263.0 4 1352.0 5 1342.0 6 1673.0 7 2048.0 8 2373.0 9 2945.0 10 3633.0 11 4473.0 12 5439.0 13 6503.0 14 7556.0 15 8947.0 16 10862.0 17 14242.0 18 13220.0 19 10741.0 20 10660.0 21 11227.0 22 12108.0 23 13396.0 24 14593.0 25 16392.0 26 18845.0 27 21933.0 28 25443.0 29 30007.0 30 35905.0 31 43526.0 32 54341.0 33 70736.0 34 96593.0 35 140892.0 36 207382.0 37 289155.0 38 218159.0 39 22897.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74246893944044 18.5910890180842 14.386179335631995 23.28026270684336 2 15.479632099168969 19.735047676860347 40.517022349000385 24.2682978749703 3 19.560142979284485 23.788951038866056 29.366516613959366 27.284389367890093 4 12.97080900063254 16.536255065997906 36.612888049773666 33.880047883595886 5 14.072368570178446 36.82961123135372 33.99525913673977 15.102761061728073 6 32.32921682897704 35.58946354440782 17.3428415560359 14.738478070579248 7 28.957938404901235 32.41977801587956 20.173287727684826 18.448995851534384 8 26.30061316410947 35.09744770181917 19.421349977372877 19.180589156698485 9 26.263311569700846 17.156371287387074 20.179337092755926 36.40098005015616 10-11 25.366388236562788 26.53161169612494 26.70373878443658 21.39826128287569 12-13 26.087857239544004 23.768329644672352 27.46909529663087 22.67471781915277 14-15 22.82058158112411 25.35326028174295 25.826311276962997 25.999846860169946 16-17 22.363515680987124 28.579802151004337 26.544379701952565 22.512302466055974 18-19 22.256140273767894 27.04961940054556 28.884765308449005 21.809475017237542 20-21 22.823138713455112 26.191226822994473 29.16114608339086 21.824488380159558 22-23 22.549909396121805 26.050992968369858 29.073319947928095 22.325777687580246 24-25 22.41578880481413 26.249574951140847 29.1355450757875 22.199091168257524 26-27 22.137947991349062 26.58103180778928 29.023637611115728 22.257382589745934 28-29 21.980656338371567 26.744938729046225 29.180083548959246 22.09432138362296 30-31 22.215823296234444 26.428949427994397 28.99592609343508 22.35930118233608 32-33 22.021238016612934 26.505521389805036 28.940832734650478 22.532407858931546 34-35 22.573274979841397 26.620037446237998 28.72611359974285 22.080573974177756 36-37 22.484765755788498 26.40375683587881 28.76434783355225 22.347129574780435 38-39 22.23840357976488 26.65316770672514 28.848945882971705 22.25948283053827 40-41 22.403992990705543 26.500564423985413 28.8526400210938 22.242802564215243 42-43 22.37041996034709 26.45497849988413 28.63571679927217 22.538884740496606 44-45 22.349634718806936 26.606248704250607 28.610592811353992 22.433523765588465 46-47 22.47234787814461 26.859511224588573 28.214569892879137 22.45357100438768 48-49 22.49820900369115 26.81911846938555 28.19716440745398 22.485508119469323 50-51 22.380629389016622 27.03318438109073 28.08599915283269 22.50018707705996 52-53 22.720097046287496 27.008848196635437 27.80466070699758 22.466394050079483 54-55 22.40714634120388 26.875222902672498 28.050947613109265 22.666683143014353 56-57 22.397424473134638 27.16324073171435 28.070846897710748 22.36848789744026 58-59 22.59240508067049 26.7898105131642 28.065607306939157 22.55217709922616 60-61 22.48869968062524 26.865410862037244 28.297791563343576 22.34809789399394 62-63 22.335415315872233 27.018245635954308 28.21649826715027 22.429840781023195 64-65 22.20748925805586 26.820707186856623 28.14914534696531 22.822658208122206 66-67 22.151716260334492 27.069369812160037 27.99950850236001 22.77940542514546 68-69 22.216085973083644 26.8722863115117 28.138371083278702 22.773256632125953 70-71 22.19037417733513 26.942656353530463 27.970983146742473 22.895986322391934 72-73 22.312216476901728 26.866471992535278 28.010863393275848 22.810448137287146 74-75 22.234389594768135 27.146659215199893 27.697280500694628 22.921670689337343 76-77 22.32238568053467 26.92643414846625 27.798214357428513 22.952965813570568 78-79 22.365749796383888 27.055321459473614 27.74804526816062 22.830883475981878 80-81 22.302621507911066 27.21548617923236 27.718521345701447 22.763370967155126 82-83 22.369050634692787 27.169045691976805 27.68789357234111 22.774010100989297 84-85 22.475099459720315 26.962911503759578 27.693203788856735 22.86878524766337 86-87 22.426718275359928 26.977428518379078 27.745758773746697 22.8500944325143 88-89 22.34938683585328 26.957147173127893 27.65294771968854 23.040518271330285 90-91 22.363768149282233 27.009249623082752 27.700490619043876 22.92649160859114 92-93 22.523696190312396 26.751163139572288 27.75623656678067 22.968904103334648 94-95 22.473320196442188 26.67197498702964 27.742291690487153 23.11241312604102 96-97 22.506011554819473 26.622639151529633 27.678145955469862 23.19320333818103 98-99 22.47908015862554 26.582685209596622 27.455489553124174 23.48274507865366 100-101 22.78997866301276 26.125210679975098 27.70724842241391 23.37756223459823 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1132.0 1 718.0 2 490.5 3 660.5 4 504.0 5 483.0 6 900.0 7 1776.5 8 2827.5 9 3225.5 10 2553.0 11 1811.0 12 1679.0 13 1777.5 14 1879.0 15 1923.5 16 1957.5 17 2030.0 18 2130.5 19 2227.0 20 2464.5 21 2846.0 22 3410.5 23 4127.0 24 5035.5 25 6114.5 26 7524.0 27 9315.0 28 11053.0 29 12861.5 30 15106.5 31 17397.0 32 19694.5 33 21973.5 34 24367.5 35 26879.5 36 29859.0 37 33178.5 38 35795.5 39 37809.0 40 39888.5 41 43120.5 42 46534.0 43 49165.5 44 51582.5 45 54307.0 46 55569.0 47 56820.5 48 60229.0 49 61031.5 50 59278.0 51 57179.0 52 54324.5 53 52492.0 54 51012.5 55 47108.5 56 41905.0 57 37787.5 58 34166.0 59 29834.0 60 24780.5 61 19946.0 62 15874.0 63 12359.0 64 9722.0 65 7615.5 66 5833.0 67 4774.0 68 3739.5 69 2671.5 70 1944.5 71 1419.0 72 1095.5 73 883.0 74 656.0 75 505.5 76 349.0 77 195.5 78 117.0 79 88.0 80 55.5 81 30.5 82 23.5 83 15.0 84 10.5 85 10.5 86 10.0 87 7.0 88 4.5 89 1.5 90 1.0 91 1.0 92 0.0 93 2.0 94 2.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49299525329921784 2 0.05147521091109751 3 0.05881900766774742 4 0.06732957587171554 5 0.07954635926128269 6 0.10775477484056412 7 0.07220256250463276 8 0.054563723565763354 9 0.05216154705657881 10-11 0.07625194576297244 12-13 0.11331409761896265 14-15 0.05672568242402946 16-17 0.0618732035151392 18-19 0.06094664971873945 20-21 0.07529107515929861 22-23 0.06509898339890131 24-25 0.08901779806892463 26-27 0.06760411032990805 28-29 0.06722662544989334 30-31 0.07127600870823302 32-33 0.07124169190095894 34-35 0.07261436419192155 36-37 0.06859929774085595 38-39 0.04142038637979646 40-41 0.0452981856017658 42-43 0.04286169228530719 44-45 0.022374558342690386 46-47 0.029718355099340295 48-49 0.02886043491748867 50-51 0.026973010517415096 52-53 0.02659552563740038 54-55 0.025977823106467207 56-57 0.02594350629919314 58-59 0.021585271775386886 60-61 0.028551583652022084 62-63 0.02110483647354998 64-65 0.023266795331816074 66-67 0.016472067491551202 68-69 0.019011511229832013 70-71 0.04234694017619621 72-73 0.03850345776150094 74-75 0.03263528371763582 76-77 0.02316384490999388 78-79 0.015545513695151449 80-81 0.017501571709773154 82-83 0.022168657499045993 84-85 0.018290858277076646 86-87 0.010397992604041695 88-89 0.043376444394418165 90-91 0.05425487230029677 92-93 0.0344197576958872 94-95 0.055421643747614976 96-97 0.044611849456284505 98-99 0.034316807274065005 100-101 0.17127518510485845 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1457012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.73418881339292 #Duplication Level Percentage of deduplicated Percentage of total 1 82.99133776365451 53.723769286684785 2 10.221793937480854 13.233990775209614 3 2.8901435547035894 5.612732957039783 4 1.259767369296187 3.262000749798827 5 0.6532739666396802 2.1144580151663606 6 0.42392481285598904 1.6465457326861115 7 0.26667817299511637 1.2084236642153567 8 0.20740703741806082 1.0741061057157761 9 0.1627670795512858 0.9482935374249734 >10 0.8386686813762123 9.836630335876762 >50 0.05689516498789142 2.519107488317739 >100 0.02542747909896444 2.988638783632043 >500 0.0013830410689580556 0.5667251639268659 >1k 3.191633236057052E-4 0.4042625728825741 >5k 2.1277554907047012E-4 0.8603148314223368 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6876 0.47192473363294196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5616 0.38544637930229814 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3405 0.23369745753638269 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 6.863361454813001E-5 0.0 6 0.0 0.0 0.0 6.863361454813001E-5 0.0 7 0.0 0.0 0.0 6.863361454813001E-5 0.0 8 0.0 0.0 0.0 6.863361454813001E-5 0.0 9 0.0 0.0 0.0 6.863361454813001E-5 0.0 10-11 0.0 0.0 0.0 6.863361454813001E-5 0.0 12-13 0.0 0.0 0.0 1.0295042182219502E-4 0.0 14-15 0.0 0.0 0.0 1.3726722909626003E-4 0.0 16-17 6.863361454813001E-5 0.0 0.0 1.3726722909626003E-4 0.0 18-19 6.863361454813001E-5 0.0 0.0 2.0590084364439001E-4 0.0 20-21 6.863361454813001E-5 0.0 0.0 2.0590084364439001E-4 0.0 22-23 6.863361454813001E-5 0.0 0.0 3.088512654665851E-4 6.863361454813001E-5 24-25 6.863361454813001E-5 0.0 0.0 6.177025309331701E-4 1.3726722909626003E-4 26-27 6.863361454813001E-5 0.0 0.0 0.0014069890982366653 1.3726722909626003E-4 28-29 6.863361454813001E-5 0.0 0.0 0.003946432836517476 1.3726722909626003E-4 30-31 6.863361454813001E-5 0.0 0.0 0.0102950421822195 1.3726722909626003E-4 32-33 6.863361454813001E-5 0.0 0.0 0.025016952502793385 1.3726722909626003E-4 34-35 6.863361454813001E-5 0.0 0.0 0.04718561000183938 1.3726722909626003E-4 36-37 6.863361454813001E-5 0.0 0.0 0.08709605686157698 1.3726722909626003E-4 38-39 6.863361454813001E-5 0.0 0.0 0.14196863169280693 1.3726722909626003E-4 40-41 6.863361454813001E-5 0.0 0.0 0.19742459224769598 1.3726722909626003E-4 42-43 6.863361454813001E-5 0.0 0.0 0.25500819485357706 1.71584036370325E-4 44-45 6.863361454813001E-5 0.0 0.0 0.3284461624200762 2.0590084364439001E-4 46-47 6.863361454813001E-5 0.0 0.0 0.41670899072897133 2.0590084364439001E-4 48-49 6.863361454813001E-5 0.0 0.0 0.504697284579674 2.0590084364439001E-4 50-51 6.863361454813001E-5 0.0 0.0 0.5983478516305974 2.0590084364439001E-4 52-53 6.863361454813001E-5 0.0 0.0 0.6873999665067961 2.0590084364439001E-4 54-55 6.863361454813001E-5 0.0 0.0 0.7852715008524296 2.0590084364439001E-4 56-57 6.863361454813001E-5 0.0 0.0 0.9039733372134204 2.0590084364439001E-4 58-59 6.863361454813001E-5 0.0 0.0 1.0290924165346613 2.0590084364439001E-4 60-61 6.863361454813001E-5 0.0 0.0 1.156098920255976 2.0590084364439001E-4 62-63 6.863361454813001E-5 0.0 0.0 1.2857821349446676 2.0590084364439001E-4 64-65 1.3726722909626003E-4 0.0 0.0 1.4219512262081575 2.0590084364439001E-4 66-67 1.3726722909626003E-4 0.0 0.0 1.5750728202650355 2.0590084364439001E-4 68-69 1.3726722909626003E-4 0.0 0.0 1.7413377515078805 2.0590084364439001E-4 70-71 1.3726722909626003E-4 0.0 0.0 1.9224618603003956 2.7453445819252006E-4 72-73 1.3726722909626003E-4 0.0 0.0 2.1036546027074587 5.490689163850401E-4 74-75 1.3726722909626003E-4 0.0 0.0 2.281106813121649 5.490689163850401E-4 76-77 1.3726722909626003E-4 0.0 0.0 2.4826837390495067 5.490689163850401E-4 78-79 1.3726722909626003E-4 0.0 0.0 2.7004925148179977 5.490689163850401E-4 80-81 1.3726722909626003E-4 0.0 0.0 2.930003321866944 5.490689163850401E-4 82-83 1.3726722909626003E-4 0.0 0.0 3.1620878894614455 5.490689163850401E-4 84-85 1.3726722909626003E-4 0.0 0.0 3.4005210664016494 5.490689163850401E-4 86-87 1.3726722909626003E-4 0.0 0.0 3.640567133283734 5.490689163850401E-4 88-89 1.3726722909626003E-4 0.0 0.0 3.904634965257664 5.490689163850401E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4185 0.0 40.029522 1 GTATCAA 6290 0.0 31.944803 1 ATCAACG 7475 0.0 26.632544 3 GACCGTA 90 8.89716E-4 26.415007 7 TATCAAC 7580 0.0 26.263624 2 TCAACGC 7600 0.0 26.257025 4 CAACGCA 7665 0.0 25.91039 5 AACGCAG 7955 0.0 25.08528 6 GTACATG 5375 0.0 24.68511 1 TACATGG 5270 0.0 24.071905 2 TATATCG 100 0.001651283 23.756355 5 GAGTACT 4980 0.0 23.102793 12-13 ACGCAGA 8850 0.0 22.24233 7 CGCAGAG 8840 0.0 22.213709 8 ACATGGG 5400 0.0 22.1726 3 GTACTTT 5385 0.0 21.040705 14-15 AGTACTT 5195 0.0 20.956964 12-13 GCAGAGT 9530 0.0 20.155647 9 CATGGGG 3355 0.0 19.968084 4 AGAGTAC 7970 0.0 19.179222 10-11 >>END_MODULE