FastQCFastQC Report
Thu 26 May 2016
SRR938197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457012
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64690.44399085251185305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57210.39265290882985177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36400.24982635695519323No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15910.10919608074607483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40850.037.0963861
GTATCAA61600.034.471471
ATCAACG74550.028.162933
TATCAAC76050.027.7967342
TCAACGC75700.027.670444
CAACGCA76400.027.4790885
AACGCAG77950.026.993616
GTACATG52150.024.6858431
TACATGG51100.024.6353072
ACGCAGA88200.023.8565987
ACATGGG51200.023.7505233
CGCAGAG88800.023.6419168
GCAGAGT96100.021.5988889
GAGTACT53350.021.27829612-13
CATGGGG33450.020.731474
AGAGTAC80000.020.24593210-11
GTACTTT56750.019.79423114-15
AGTACTT54400.019.5579112-13
CAGAGTA92950.018.6005310-11
CCTACAC6150.018.5369933
Produced by FastQC (version 0.11.5)