##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938197_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1457012 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48707766305288 34.0 31.0 34.0 31.0 34.0 2 32.70463592612827 34.0 31.0 34.0 31.0 34.0 3 32.765830343195525 34.0 31.0 34.0 31.0 34.0 4 36.228092150236236 37.0 35.0 37.0 35.0 37.0 5 36.189604478206086 37.0 35.0 37.0 35.0 37.0 6 36.22730560901351 37.0 37.0 37.0 35.0 37.0 7 36.19255709630394 37.0 37.0 37.0 35.0 37.0 8 36.18659763955273 37.0 37.0 37.0 35.0 37.0 9 37.889599399318605 39.0 38.0 39.0 35.0 39.0 10-11 37.87586341087101 39.0 38.0 39.0 35.0 39.0 12-13 37.81886388032494 39.0 38.0 39.0 35.0 39.0 14-15 39.13985300052436 40.0 38.5 41.0 36.0 41.0 16-17 39.0929861936621 40.0 38.0 41.0 36.0 41.0 18-19 39.11144726330325 40.0 38.5 41.0 36.0 41.0 20-21 39.04149348117929 40.0 39.0 41.0 35.5 41.0 22-23 38.889514979972716 40.0 38.0 41.0 35.0 41.0 24-25 38.86132612497357 40.0 38.0 41.0 35.0 41.0 26-27 38.83401166222379 40.0 38.0 41.0 35.0 41.0 28-29 38.77808830675382 40.0 38.0 41.0 35.0 41.0 30-31 38.59313204009301 40.0 38.0 41.0 34.5 41.0 32-33 38.4381666039813 40.0 38.0 41.0 34.0 41.0 34-35 38.25125530881009 40.0 38.0 41.0 34.0 41.0 36-37 38.13640004337644 40.0 38.0 41.0 34.0 41.0 38-39 38.14032760196896 40.0 38.0 41.0 34.0 41.0 40-41 38.09210253587479 40.0 38.0 41.0 33.5 41.0 42-43 37.95217026352563 40.0 38.0 41.0 33.0 41.0 44-45 37.74991729649447 40.0 37.0 41.0 33.0 41.0 46-47 37.44543112891314 40.0 37.0 41.0 32.5 41.0 48-49 37.14194941428073 40.0 37.0 41.0 31.5 41.0 50-51 36.864766728070876 40.0 36.0 41.0 31.0 41.0 52-53 36.46395602781583 39.0 36.0 41.0 30.0 41.0 54-55 36.24786480825141 39.0 35.0 41.0 30.0 41.0 56-57 36.01909696007994 39.0 35.0 40.5 29.0 41.0 58-59 35.69816308994024 38.5 35.0 40.0 28.5 41.0 60-61 35.346395911632854 38.0 35.0 40.0 28.0 41.0 62-63 34.99057385937796 38.0 34.0 40.0 28.0 41.0 64-65 34.59912924533222 37.0 34.0 40.0 26.5 41.0 66-67 34.17970922682861 36.5 34.0 39.0 26.0 41.0 68-69 33.731412644508076 36.0 33.0 39.0 26.0 40.5 70-71 33.21981974067475 35.5 33.0 38.5 25.0 40.0 72-73 32.79099142628887 35.0 32.0 37.5 24.5 40.0 74-75 32.329329820207384 35.0 32.0 37.0 23.5 39.0 76-77 31.037751576514125 34.0 30.5 35.5 21.5 38.5 78-79 31.753430308055115 35.0 32.0 36.0 23.0 38.5 80-81 31.582415930685542 35.0 32.0 36.0 22.5 37.5 82-83 31.209734031016904 35.0 31.5 35.5 20.5 37.0 84-85 30.814012856448677 34.0 31.0 35.0 19.5 36.5 86-87 30.371295157486692 34.0 31.0 35.0 18.0 36.0 88-89 29.9067481942496 34.0 30.0 35.0 13.0 36.0 90-91 29.554430917521614 34.0 30.0 35.0 7.0 35.0 92-93 29.13100269592838 34.0 29.0 35.0 2.0 35.0 94-95 28.690803850620313 34.0 29.0 35.0 2.0 35.0 96-97 28.090941941452783 34.0 29.0 35.0 2.0 35.0 98-99 27.38402909516188 33.5 27.0 35.0 2.0 35.0 100-101 24.943590375370967 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2183876045257378 1101 2 0.15683520923722938 1101 3 0.17589730226616496 1101 4 0.09384384398818213 1101 5 0.08490466370921013 1101 6 0.014429737668677944 1101 7 0.09062715960978807 1101 8 0.12294703195265555 1101 9 0.028577932827481334 1101 10-11 0.13831415929649182 1101 12-13 0.07367013107719345 1101 14-15 0.1623539264611935 1101 16-17 0.163105149235669 1101 18-19 0.19407997996388104 1101 20-21 0.2494386837419711 1101 22-23 0.22377580015933773 1101 24-25 0.1631554257939598 1101 26-27 0.18051158418149527 1101 28-29 0.15655220022426164 1101 30-31 0.27392267008738713 1101 32-33 0.15242327538165057 1101 34-35 0.10569172565072193 1101 36-37 0.26565360701053464 1101 38-39 0.32397841651764026 1101 40-41 0.27531613723200365 1101 42-43 0.26014256673519753 1101 44-45 0.44500313190035 1101 46-47 0.5950299900688236 1101 48-49 0.6429989197347723 1101 50-51 0.5933083722457937 1101 52-53 0.44849197573932287 1101 54-55 0.5028862543768255 1101 56-57 0.5773704187243709 1101 58-59 0.4863038456876012 1101 60-61 0.4914610837748157 1101 62-63 0.5316540428098335 1101 64-65 0.5642113088543041 1101 66-67 0.3903814453716379 1101 68-69 0.47253232957216085 1101 70-71 0.6680735195380549 1101 72-73 0.5499777919765378 1101 74-75 0.597782799374798 1101 76-77 0.45983057242433745 1101 78-79 0.5781612849614959 1101 80-81 0.6084506685815221 1101 82-83 0.7714304074370624 1101 84-85 1.0167682248383265 1101 86-87 0.880908062046533 1101 88-89 0.7009024166079811 1101 90-91 0.8436372193793993 1101 92-93 1.0109124755523204 1101 94-95 0.9418308931037664 1101 96-97 0.9141217924538516 1101 98-99 0.8663158099984933 1101 100-101 1.2560833926733146 1102 1 0.14614656730625342 1102 2 0.18866676369809454 1102 3 0.17491037013699184 1102 4 0.13260102789622863 1102 5 0.07593333092322041 1102 6 0.11596743608137672 1102 7 0.17892594176633025 1102 8 0.19644183217159394 1102 9 0.1269170817054217 1102 10-11 0.13295530598505678 1102 12-13 0.18827912122497992 1102 14-15 0.2126498350545134 1102 16-17 0.29929579647157567 1102 18-19 0.20380305741325344 1102 20-21 0.17429072944531043 1102 22-23 0.2781981797433488 1102 24-25 0.23396053663140037 1102 26-27 0.08089130503534392 1102 28-29 0.19127442244649018 1102 30-31 0.22496946811693874 1102 32-33 0.24226659776807224 1102 34-35 0.3132074618302525 1102 36-37 0.22304020389067603 1102 38-39 0.16165459331019605 1102 40-41 0.26745745496599227 1102 42-43 0.2162249422141258 1102 44-45 0.13557767349860228 1102 46-47 0.18687368875519184 1102 48-49 0.20701010610587645 1102 50-51 0.10445283721569609 1102 52-53 0.13733810327079965 1102 54-55 0.09893168404294528 1102 56-57 0.23559342775556757 1102 58-59 0.19746798088190332 1102 60-61 0.17617304327125538 1102 62-63 0.16606496235006318 1102 64-65 0.2871921175570904 1102 66-67 0.17265002090529435 1102 68-69 0.08369903044791016 1102 70-71 0.29212165172195625 1102 72-73 0.22520528239799376 1102 74-75 0.3633094482527426 1102 76-77 0.2191474169782488 1102 78-79 0.4449263027502184 1102 80-81 0.4752644343177046 1102 82-83 0.5541180442734124 1102 84-85 0.4418349325558957 1102 86-87 0.40122056204653234 1102 88-89 0.22070947736331803 1102 90-91 0.10319523798914076 1102 92-93 0.26114509733666935 1102 94-95 -0.15737716655687706 1102 96-97 0.27557242874285137 1102 98-99 0.16198750540079132 1102 100-101 0.4232366835873833 1103 1 0.12665251968720526 1103 2 0.06991676369809596 1103 3 0.07668463753626042 1103 4 0.03606943448964017 1103 5 0.04460320271809337 1103 6 0.020534743773680475 1103 7 0.047092150557539014 1103 8 0.035178095907859586 1103 9 0.041672118335455366 1103 10-11 0.01005008620483494 1103 12-13 0.0972649270857815 1103 14-15 0.08647080575048705 1103 16-17 0.10552862614191127 1103 18-19 0.08812654642424178 1103 20-21 0.1758417825588694 1103 22-23 0.14430967241734294 1103 24-25 0.08379455677791725 1103 26-27 0.09396364935769697 1103 28-29 0.07511141878347871 1103 30-31 -0.02590049525302618 1103 32-33 0.0760349127863833 1103 34-35 0.05150874388153426 1103 36-37 0.19273457202254463 1103 38-39 0.05898746876807337 1103 40-41 0.13965869122973373 1103 42-43 -0.01486480137562296 1103 44-45 -0.008384093900673406 1103 46-47 -0.09081060611659808 1103 48-49 0.025113357021623983 1103 50-51 -0.08454098146562927 1103 52-53 0.003306509864202667 1103 54-55 0.020451830563089857 1103 56-57 0.07723147720611934 1103 58-59 0.10217265121157482 1103 60-61 0.005271028619240781 1103 62-63 -0.0660092134741177 1103 64-65 0.01367448935196336 1103 66-67 0.11302891284668704 1103 68-69 0.07383753777391178 1103 70-71 -0.008685353772548865 1103 72-73 -0.020570816503109768 1103 74-75 -0.12758455357876386 1103 76-77 0.1050791935350297 1103 78-79 -0.010024475638061858 1103 80-81 0.16223787754114838 1103 82-83 0.257237320830189 1103 84-85 0.2677850241309834 1103 86-87 0.5461095272479994 1103 88-89 0.47982119897504205 1103 90-91 0.3158783881722904 1103 92-93 0.39255535374692485 1103 94-95 0.29549600194129155 1103 96-97 0.33143895804687773 1103 98-99 0.5347381922139824 1103 100-101 0.4988605388987395 1104 1 0.21588545424033612 1104 2 0.16402889130775833 1104 3 0.14702874334834348 1104 4 0.06330197206700205 1104 5 0.03853669123286352 1104 6 0.09651590546912558 1104 7 0.08662371915314537 1104 8 0.04458281270174069 1104 9 0.1379531771780904 1104 10-11 0.18997574347039148 1104 12-13 0.24889884325171607 1104 14-15 0.237731203808913 1104 16-17 0.19328733705332724 1104 18-19 0.2549682433151972 1104 20-21 0.2771030828349481 1104 22-23 0.23331524812243032 1104 24-25 0.18745756044091877 1104 26-27 0.24445716241277893 1104 28-29 0.40105919842584115 1104 30-31 0.3685743238523429 1104 32-33 0.4348613080659902 1104 34-35 0.3425515270425379 1104 36-37 0.42054452889653504 1104 38-39 0.47069825387786324 1104 40-41 0.41057299859723884 1104 42-43 0.4590556952049809 1104 44-45 0.5041946245893811 1104 46-47 0.5386377780115836 1104 48-49 0.6116125720922696 1104 50-51 0.6879052914732569 1104 52-53 0.8273488058276826 1104 54-55 0.7368920405993578 1104 56-57 0.7076073410795161 1104 58-59 0.6996121575180823 1104 60-61 0.5355223864568117 1104 62-63 0.6639317814915131 1104 64-65 0.5714128101444729 1104 66-67 0.47532162834137637 1104 68-69 0.543218075134746 1104 70-71 0.5140036157144579 1104 72-73 0.4629889734606252 1104 74-75 0.36981345071579597 1104 76-77 0.4553110634713313 1104 78-79 0.430479683585304 1104 80-81 0.4963543357955338 1104 82-83 0.3631342153970323 1104 84-85 0.5623229984719167 1104 86-87 0.6101128474089563 1104 88-89 0.5474893272342989 1104 90-91 0.6490403001519347 1104 92-93 0.7921385291921688 1104 94-95 0.6615040497227405 1104 96-97 0.7729536010393545 1104 98-99 0.7951921881359567 1104 100-101 1.0194112525528993 1105 1 0.1084707015053894 1105 2 0.14888691635245976 1105 3 0.16549407371411462 1105 4 0.07313061891924377 1105 5 0.05223416824379967 1105 6 0.06526895692894641 1105 7 0.0804517733908412 1105 8 0.08499845099137104 1105 9 0.06879236650834031 1105 10-11 0.09296541648858891 1105 12-13 0.14294205697343898 1105 14-15 0.19602090477426515 1105 16-17 0.13995034477415658 1105 18-19 0.15828621132074971 1105 20-21 0.14751747528718795 1105 22-23 0.17341740678823925 1105 24-25 0.14473712298364205 1105 26-27 0.08336197033496262 1105 28-29 0.2250947687668301 1105 30-31 0.175200158479214 1105 32-33 0.15126494591115858 1105 34-35 0.1807812257855943 1105 36-37 0.18880867335980156 1105 38-39 0.07817529848221483 1105 40-41 0.14372304792566126 1105 42-43 0.0899006086529539 1105 44-45 0.047600272610011984 1105 46-47 0.12725553826533798 1105 48-49 0.33815294900858106 1105 50-51 0.10864478014119072 1105 52-53 0.21635311238448907 1105 54-55 0.33392257034962114 1105 56-57 0.3442083599724768 1105 58-59 0.19636792961738792 1105 60-61 0.0699344353879141 1105 62-63 0.08807880377179345 1105 64-65 0.2172498613483853 1105 66-67 0.2412287831518256 1105 68-69 0.3051991586092271 1105 70-71 0.3506255108014784 1105 72-73 0.08707942798924506 1105 74-75 0.03289356479409378 1105 76-77 0.0856424898877961 1105 78-79 0.2432555776419889 1105 80-81 0.3993288567373874 1105 82-83 0.5128518817079062 1105 84-85 0.6592356787395346 1105 86-87 0.6772853689501517 1105 88-89 0.4467717922414245 1105 90-91 0.18301125530515705 1105 92-93 0.2087128804307703 1105 94-95 0.22401309887944265 1105 96-97 0.504071588574245 1105 98-99 0.494450409820935 1105 100-101 0.32488889650604236 1106 1 -0.08638757373435624 1106 2 -0.020286947214060547 1106 3 0.0532778445109372 1106 4 0.0025913775783408255 1106 5 0.0152762172603218 1106 6 -0.02156784029075709 1106 7 0.052249300401335574 1106 8 -0.029699986953872326 1106 9 -0.04346576830106841 1106 10-11 -0.039434647952923285 1106 12-13 -0.27213227425963993 1106 14-15 -0.1944820742487181 1106 16-17 -0.10202868938543475 1106 18-19 -0.10255500181861521 1106 20-21 -0.07729772098935683 1106 22-23 0.08222475461035827 1106 24-25 -0.012036406083012707 1106 26-27 -0.044493896729278504 1106 28-29 0.045214022809794585 1106 30-31 0.03698289746688488 1106 32-33 -0.07181070208919493 1106 34-35 -0.06870589615599698 1106 36-37 0.08206345385823255 1106 38-39 0.10803653505146826 1106 40-41 0.1043680962661071 1106 42-43 0.0035205825410997704 1106 44-45 0.052650829395837206 1106 46-47 0.056121004507112104 1106 48-49 0.03092525629401166 1106 50-51 0.14644084324917372 1106 52-53 0.05253897585416212 1106 54-55 -0.24543058402505125 1106 56-57 -0.042638820525212395 1106 58-59 -0.051273017561065615 1106 60-61 -0.07526639490183129 1106 62-63 0.13788035744543237 1106 64-65 -0.12991550376118965 1106 66-67 -0.11757213914400211 1106 68-69 -0.2542455734835869 1106 70-71 -0.28863589823262714 1106 72-73 -0.09755527618511195 1106 74-75 -0.16835717786636906 1106 76-77 -0.1542692010313509 1106 78-79 -0.1944589557809877 1106 80-81 -0.3387804274962356 1106 82-83 -0.5391099059666296 1106 84-85 -0.2890164849866004 1106 86-87 -0.13196659735401184 1106 88-89 -0.11890872686660359 1106 90-91 -0.31623677187202404 1106 92-93 -0.031008139980055915 1106 94-95 -0.16033511063368877 1106 96-97 0.34459957127561225 1106 98-99 0.32274335235810625 1106 100-101 0.30719262461501984 1107 1 0.05815988297395336 1107 2 0.12645304859945128 1107 3 0.08223662549139732 1107 4 0.08848237501813827 1107 5 0.03512893874612644 1107 6 0.07596034246914485 1107 7 0.043803865194398384 1107 8 0.053791830608268754 1107 9 -0.028738364156438934 1107 10-11 0.06679369278429448 1107 12-13 0.09537185408306215 1107 14-15 0.17986825968635145 1107 16-17 0.06332351236752487 1107 18-19 0.15961332185060684 1107 20-21 0.18635890824342738 1107 22-23 0.18494087312733853 1107 24-25 0.09731592269586997 1107 26-27 -0.029775885347042674 1107 28-29 -0.027550812834753913 1107 30-31 0.10111265355921262 1107 32-33 0.10320150850189691 1107 34-35 0.1778494200580596 1107 36-37 0.13994210294642073 1107 38-39 0.18752233945658503 1107 40-41 0.2003370804701703 1107 42-43 0.20633492251616303 1107 44-45 0.24521721833491483 1107 46-47 0.29264887596402644 1107 48-49 0.4250715078661855 1107 50-51 0.6298630649147867 1107 52-53 0.4686513204486715 1107 54-55 0.5684037473265633 1107 56-57 0.5545170062972957 1107 58-59 0.3910869542427591 1107 60-61 0.3612293622532192 1107 62-63 0.3466501560467137 1107 64-65 0.3629064130421966 1107 66-67 0.2175012406939345 1107 68-69 0.43574518582732935 1107 70-71 0.4716176437333388 1107 72-73 0.28863888384250913 1107 74-75 0.35053435093541196 1107 76-77 0.39143083707642745 1107 78-79 0.5323040209911554 1107 80-81 0.5716642795479849 1107 82-83 0.53592551816579 1107 84-85 0.618585569613078 1107 86-87 0.7270012990787045 1107 88-89 0.7730830039243024 1107 90-91 0.6184982896103115 1107 92-93 0.8072516226782547 1107 94-95 0.6749795737426574 1107 96-97 0.9004871783754673 1107 98-99 1.1255787589573707 1107 100-101 1.225258216511122 1108 1 0.053709363367090646 1108 2 0.06478088735803311 1108 3 0.020510750341429684 1108 4 -2.7964400603508466E-4 1108 5 0.04418080661506707 1108 6 0.1279695864179473 1108 7 0.06787586073915008 1108 8 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-0.326035473944831 2316 86-87 -0.2782063715359868 2316 88-89 -0.41661458682244756 2316 90-91 -0.4855137217304595 2316 92-93 -0.6845920531877177 2316 94-95 -0.312915636257582 2316 96-97 -0.1830426500517497 2316 98-99 -0.11080606609623445 2316 100-101 -0.1423869044763677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 10.0 5 27.0 6 44.0 7 100.0 8 267.0 9 502.0 10 868.0 11 1421.0 12 2146.0 13 3308.0 14 4394.0 15 6142.0 16 7906.0 17 10908.0 18 11052.0 19 7882.0 20 6949.0 21 6997.0 22 7191.0 23 7831.0 24 8926.0 25 10813.0 26 12994.0 27 15886.0 28 19384.0 29 23896.0 30 29396.0 31 36397.0 32 46070.0 33 59431.0 34 83321.0 35 126408.0 36 204288.0 37 346056.0 38 314166.0 39 33627.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03423719228119 19.0193354618905 14.869198057394172 23.077229288434136 2 15.766723390059015 20.06923503048167 39.94468611187574 24.219355467583576 3 19.665527276720756 23.787030173242183 29.63779754550696 26.909645004530102 4 13.199754017125459 16.91180305996107 36.31795757344483 33.57048534946864 5 14.110865250251885 36.86709512344442 33.9883954284522 15.033644197851492 6 32.39568376924829 35.85076855921571 17.08318119548775 14.67036647604824 7 28.85865044351042 32.43199095134426 20.114453415620464 18.594905189524862 8 26.281447215349296 35.392328784046725 19.233839279892656 19.092384720711323 9 26.00835275102007 17.072213204484544 20.244611377448944 36.67482266704644 10-11 25.384348241469528 26.580151707741596 26.661757075439326 21.37374297534955 12-13 25.93108361495993 23.782679895567092 27.52358937332019 22.762647116152785 14-15 22.68056131315322 25.319420842107 25.90922380872635 26.09079403601343 16-17 22.28506795094327 28.660569235425776 26.57132052213888 22.48304229149208 18-19 22.090174960810206 27.03522688900297 28.99272620953019 21.88187194065663 20-21 22.729840248398776 26.187464482104474 29.186410269785014 21.89628499971174 22-23 22.475243855232492 26.042098486491533 29.20857892728406 22.27407873099192 24-25 22.381219083467002 26.225010244023977 29.29214309099782 22.101627581511202 26-27 22.10427354299801 26.609606461697517 29.077947727305492 22.20817226799899 28-29 21.938914003080964 26.75177924127572 29.213461939044404 22.09584481659891 30-31 22.146797692812413 26.37421654728993 29.14543600189978 22.333549757997876 32-33 21.9803521405405 26.473821099262974 29.049827265342877 22.49599949485365 34-35 22.5243462893039 26.566655786218764 28.795328477976362 22.113669446500975 36-37 22.39158269853183 26.391956080899053 28.875497914652616 22.340963305916496 38-39 22.08360762982433 26.63597978248946 28.995913525728724 22.284499061957487 40-41 22.383995903355434 26.395934382297664 28.95702367240023 22.263046041946673 42-43 22.270765287719477 26.40702342273456 28.77067794452111 22.55153334502485 44-45 22.331829300768995 26.519967439319352 28.722240372498582 22.42596288741307 46-47 22.451736842249755 26.881418958800612 28.252752894279524 22.414091304670105 48-49 22.44240267067121 26.849298427192085 28.231304889733234 22.476994012403466 50-51 22.354105525555042 26.9782953057353 28.20663110530318 22.46096806340648 52-53 22.681796718215086 27.025377965315318 27.882097058912347 22.410728257557246 54-55 22.29092828336349 26.830458499998628 28.16256832476328 22.716044891874603 56-57 22.387255561381785 27.172905919786523 28.03762082947841 22.40221768935328 58-59 22.57874334597107 26.805716081954028 28.048533574191563 22.56700699788334 60-61 22.431764460416247 26.877026407469533 28.293006509212006 22.398202622902215 62-63 22.30589041133498 27.01528882397674 28.148841601853658 22.529979162834625 64-65 22.10043568618515 26.87634007132405 28.109411590295757 22.91381265219504 66-67 22.087738467493747 27.128877456053896 27.95663316431162 22.826750912140735 68-69 22.128346496143813 26.94084250680675 28.103155162390898 22.827655834658536 70-71 22.11999626633137 26.997169549736032 27.941773986761948 22.94106019717065 72-73 22.291648936316243 26.972667349342355 27.916962935102802 22.818720779238603 74-75 22.23430555136128 27.19651588319108 27.638584994495584 22.930593570952055 76-77 22.25997452320228 26.98131518477542 27.79929060296003 22.959419689062273 78-79 22.252870944096546 27.12228862905728 27.797643396210876 22.8271970306353 80-81 22.21272027958589 27.265286764968305 27.732063977510137 22.789928977935666 82-83 22.311121878970027 27.240219874798477 27.69522673473296 22.75343151149854 84-85 22.308192027091813 27.14064756929013 27.645427518657712 22.905732884960344 86-87 22.286951457763315 27.086199506592095 27.712859799472824 22.913989236171762 88-89 22.212049188372635 27.10799712562225 27.606552043876125 23.07340164212899 90-91 22.16293790910008 27.241732892521597 27.661702844663278 22.93362635371504 92-93 22.359238123868185 26.97578634579002 27.7461662028739 22.91880932746789 94-95 22.143297378470457 26.9745890905497 27.702002454338054 23.180111076641786 96-97 22.25545234200279 26.90953365845088 27.6708179722672 23.164196027279125 98-99 22.13224736652821 26.930869478082542 27.519814524520047 23.417068630869203 100-101 22.104347802214395 26.822737218361965 27.719641293276926 23.353273686146718 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1049.0 1 682.0 2 484.0 3 625.0 4 471.5 5 434.0 6 857.5 7 1852.0 8 2882.5 9 3217.0 10 2594.5 11 1796.5 12 1677.5 13 1744.5 14 1833.5 15 1898.5 16 1877.0 17 1987.0 18 2125.0 19 2233.0 20 2452.5 21 2793.5 22 3327.0 23 4069.0 24 4887.0 25 5904.5 26 7392.0 27 9310.5 28 10997.5 29 12788.5 30 15257.0 31 17498.5 32 19844.5 33 22090.0 34 24295.0 35 26784.0 36 29886.5 37 33190.0 38 35819.5 39 37794.5 40 39801.0 41 43322.5 42 46647.5 43 49671.0 44 52340.0 45 54492.0 46 55891.5 47 57462.5 48 60818.5 49 61543.0 50 59886.5 51 57820.0 52 54975.5 53 53459.5 54 51706.5 55 47293.0 56 42055.5 57 37944.5 58 34147.0 59 29568.0 60 24319.5 61 19339.5 62 15354.0 63 11802.0 64 9018.0 65 7091.5 66 5385.0 67 4318.5 68 3336.0 69 2232.5 70 1552.0 71 1124.5 72 857.0 73 692.5 74 546.5 75 423.5 76 267.5 77 154.0 78 82.5 79 38.0 80 23.0 81 13.0 82 6.0 83 7.5 84 3.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016128899418810554 3 0.006314292538427961 4 0.0 5 0.0 6 0.0 7 0.0 8 4.8043530183691004E-4 9 4.8043530183691004E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.4316807274065007E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0044611849456284505 26-27 0.020933252437179653 28-29 3.4316807274065E-5 30-31 0.0 32-33 5.83385723659105E-4 34-35 0.018565392735269165 36-37 0.0041523336801618654 38-39 0.017501571709773154 40-41 0.014413059055107301 42-43 0.007927182480309016 44-45 0.0027453445819252006 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.0590084364439001E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.863361454813E-5 84-85 0.0027796613891992654 86-87 1.7158403637032503E-4 88-89 6.863361454813E-5 90-91 2.0590084364439001E-4 92-93 2.4021765091845502E-4 94-95 0.0 96-97 3.4316807274065E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1457012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.94484420767601 #Duplication Level Percentage of deduplicated Percentage of total 1 81.56630044966118 51.34178074400416 2 11.138313524511142 14.02198819073209 3 3.166511630291176 5.979467437514167 4 1.3238525491084345 3.3331876983026065 5 0.6934061273611551 2.182317032969793 6 0.4505160569740294 1.7014597811572063 7 0.28797219259828566 1.2688455359469368 8 0.21076173749562974 1.0613091785281217 9 0.15670170653404059 0.8877208054375936 >10 0.9110431692459379 10.380667617200459 >50 0.06472087569709106 2.7705856216322964 >100 0.02815404280937935 3.289021328019047 >500 0.0010912110703335054 0.43787395647644095 >1k 4.364844281334021E-4 0.506491381185455 >5k 2.1824221406670105E-4 0.837283690893775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6469 0.44399085251185305 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5721 0.39265290882985177 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3640 0.24982635695519323 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1591 0.10919608074607483 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 6.863361454813001E-5 0.0 6 6.863361454813001E-5 0.0 0.0 6.863361454813001E-5 0.0 7 6.863361454813001E-5 0.0 0.0 6.863361454813001E-5 0.0 8 6.863361454813001E-5 0.0 0.0 6.863361454813001E-5 0.0 9 6.863361454813001E-5 0.0 0.0 6.863361454813001E-5 0.0 10-11 6.863361454813001E-5 0.0 0.0 6.863361454813001E-5 0.0 12-13 6.863361454813001E-5 0.0 0.0 6.863361454813001E-5 0.0 14-15 6.863361454813001E-5 0.0 0.0 6.863361454813001E-5 0.0 16-17 6.863361454813001E-5 0.0 0.0 6.863361454813001E-5 6.863361454813001E-5 18-19 6.863361454813001E-5 0.0 0.0 1.3726722909626003E-4 6.863361454813001E-5 20-21 6.863361454813001E-5 0.0 0.0 1.3726722909626003E-4 6.863361454813001E-5 22-23 6.863361454813001E-5 0.0 0.0 2.4021765091845502E-4 6.863361454813001E-5 24-25 6.863361454813001E-5 0.0 0.0 5.490689163850401E-4 6.863361454813001E-5 26-27 6.863361454813001E-5 0.0 0.0 0.0013040386764144702 6.863361454813001E-5 28-29 6.863361454813001E-5 0.0 0.0 0.0036375815710508906 6.863361454813001E-5 30-31 6.863361454813001E-5 0.0 0.0 0.009883240494930721 6.863361454813001E-5 32-33 6.863361454813001E-5 0.0 0.0 0.024673784430052737 1.3726722909626003E-4 34-35 6.863361454813001E-5 0.0 0.0 0.04725424361638751 1.3726722909626003E-4 36-37 6.863361454813001E-5 0.0 0.0 0.08754217535613983 1.3726722909626003E-4 38-39 6.863361454813001E-5 0.0 0.0 0.14282655187465854 2.0590084364439001E-4 40-41 6.863361454813001E-5 0.0 0.0 0.19872863092411044 2.0590084364439001E-4 42-43 6.863361454813001E-5 0.0 0.0 0.25672403521728027 2.0590084364439001E-4 44-45 6.863361454813001E-5 0.0 0.0 0.3296815674819425 2.0590084364439001E-4 46-47 6.863361454813001E-5 0.0 0.0 0.4182532470563043 2.0590084364439001E-4 48-49 6.863361454813001E-5 0.0 0.0 0.5070308274743104 2.7453445819252006E-4 50-51 6.863361454813001E-5 0.0 0.0 0.6014706810925373 2.7453445819252006E-4 52-53 6.863361454813001E-5 0.0 0.0 0.6913807161505876 2.7453445819252006E-4 54-55 6.863361454813001E-5 0.0 0.0 0.7898013194126061 2.7453445819252006E-4 56-57 6.863361454813001E-5 0.0 0.0 0.9085717893881451 2.7453445819252006E-4 58-59 6.863361454813001E-5 0.0 0.0 1.0346517393130599 2.7453445819252006E-4 60-61 6.863361454813001E-5 0.0 0.0 1.1622759455653076 2.7453445819252006E-4 62-63 6.863361454813001E-5 0.0 0.0 1.2915473585667105 2.7453445819252006E-4 64-65 6.863361454813001E-5 0.0 0.0 1.4288489044702446 2.7453445819252006E-4 66-67 6.863361454813001E-5 0.0 0.0 1.5829656859380705 3.088512654665851E-4 68-69 6.863361454813001E-5 0.0 0.0 1.7491963003736415 3.4316807274065007E-4 70-71 6.863361454813001E-5 0.0 0.0 1.9310410621189118 3.4316807274065007E-4 72-73 6.863361454813001E-5 0.0 0.0 2.1140525953115006 5.490689163850401E-4 74-75 6.863361454813001E-5 0.0 0.0 2.2934951805475867 5.490689163850401E-4 76-77 6.863361454813001E-5 0.0 0.0 2.4973026989482587 5.490689163850401E-4 78-79 6.863361454813001E-5 0.0 0.0 2.716758681465904 5.490689163850401E-4 80-81 6.863361454813001E-5 0.0 0.0 2.9482255465294727 5.490689163850401E-4 82-83 6.863361454813001E-5 0.0 0.0 3.183879062080477 5.490689163850401E-4 84-85 6.863361454813001E-5 0.0 0.0 3.4259498205917316 5.490689163850401E-4 86-87 6.863361454813001E-5 0.0 0.0 3.669599152237593 5.490689163850401E-4 88-89 6.863361454813001E-5 0.0 0.0 3.938642921266263 5.490689163850401E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4085 0.0 37.096386 1 GTATCAA 6160 0.0 34.47147 1 ATCAACG 7455 0.0 28.16293 3 TATCAAC 7605 0.0 27.796734 2 TCAACGC 7570 0.0 27.67044 4 CAACGCA 7640 0.0 27.479088 5 AACGCAG 7795 0.0 26.99361 6 GTACATG 5215 0.0 24.685843 1 TACATGG 5110 0.0 24.635307 2 ACGCAGA 8820 0.0 23.856598 7 ACATGGG 5120 0.0 23.750523 3 CGCAGAG 8880 0.0 23.641916 8 GCAGAGT 9610 0.0 21.598888 9 GAGTACT 5335 0.0 21.278296 12-13 CATGGGG 3345 0.0 20.73147 4 AGAGTAC 8000 0.0 20.245932 10-11 GTACTTT 5675 0.0 19.794231 14-15 AGTACTT 5440 0.0 19.55791 12-13 CAGAGTA 9295 0.0 18.60053 10-11 CCTACAC 615 0.0 18.536993 3 >>END_MODULE