FastQCFastQC Report
Thu 26 May 2016
SRR938196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497819
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66020.4407742190478289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56690.37848364855833716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34040.22726377486198263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38500.044.4081041
GTATCAA60550.035.236471
ATCAACG74500.028.1523323
TATCAAC76550.027.6469272
TCAACGC76100.027.560434
CAACGCA76900.027.335565
AACGCAG78700.026.6454626
GTACATG53450.024.2353731
ACGCAGA87650.023.7571547
GTGTAGC8050.023.6642441
TACATGG53100.023.4659862
CGCAGAG89350.023.092318
ACATGGG54500.022.426873
GAGTACT52600.022.27790312-13
GCAGAGT95050.021.1065859
CCTATAC3850.021.0000483
GTACTTT56650.020.68556614-15
TGGTATC20650.020.2672422
AGAGTAC81800.020.0213610-11
AGTACTT55450.019.84689512-13
Produced by FastQC (version 0.11.5)