##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938196_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1497819 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.952312662611437 33.0 31.0 34.0 30.0 34.0 2 32.0734621472955 33.0 31.0 34.0 30.0 34.0 3 32.09646292375781 34.0 31.0 34.0 30.0 34.0 4 35.60890735128877 37.0 35.0 37.0 33.0 37.0 5 35.61202855618736 37.0 35.0 37.0 33.0 37.0 6 35.636353925274015 37.0 35.0 37.0 33.0 37.0 7 35.574106083578855 37.0 35.0 37.0 33.0 37.0 8 35.569129514313815 37.0 35.0 37.0 33.0 37.0 9 37.19902538290675 39.0 37.0 39.0 34.0 39.0 10-11 37.09710919677211 39.0 37.0 39.0 33.5 39.0 12-13 36.93673100688401 39.0 37.0 39.0 33.0 39.0 14-15 38.11328772034538 40.0 38.0 41.0 33.0 41.0 16-17 37.92393807262427 40.0 38.0 41.0 33.0 41.0 18-19 38.09928769764571 40.0 38.0 41.0 33.0 41.0 20-21 38.102057725265865 40.0 38.0 41.0 33.0 41.0 22-23 37.983861868490116 40.0 38.0 41.0 33.0 41.0 24-25 37.85433219901737 40.0 38.0 41.0 33.0 41.0 26-27 37.668539055787114 40.0 38.0 41.0 32.0 41.0 28-29 37.47872506624632 40.0 37.0 41.0 32.0 41.0 30-31 37.25645388394726 40.0 37.0 41.0 31.5 41.0 32-33 36.974371402686174 40.0 36.0 41.0 30.5 41.0 34-35 36.729468981232046 39.0 36.0 41.0 30.0 41.0 36-37 36.462340242712905 39.0 35.5 41.0 30.0 41.0 38-39 36.17035002226571 39.0 35.0 40.5 29.5 41.0 40-41 36.046349058197286 39.0 35.0 40.0 29.0 41.0 42-43 35.82721209972634 39.0 35.0 40.5 28.5 41.0 44-45 35.485696202278106 39.0 35.0 40.0 26.5 41.0 46-47 35.095365661672076 38.0 34.5 40.0 25.0 41.0 48-49 34.69359014674002 38.0 34.0 40.0 24.0 41.0 50-51 33.97261551629403 37.5 33.5 39.5 23.0 40.5 52-53 34.05745854472403 38.0 33.5 40.0 23.0 40.5 54-55 34.13212344081629 38.0 34.0 40.0 22.5 41.0 56-57 33.79920203976582 38.0 33.0 40.0 21.0 41.0 58-59 33.37895166238377 37.5 33.0 40.0 18.5 41.0 60-61 32.85336979968875 37.0 32.0 40.0 15.0 41.0 62-63 32.28702266428721 36.0 31.0 39.0 9.5 41.0 64-65 31.720687212540366 35.5 31.0 39.0 7.5 40.0 66-67 31.11200986233984 35.0 30.0 38.5 2.0 40.0 68-69 30.40084850038623 35.0 29.0 38.0 2.0 40.0 70-71 29.778013565056927 34.5 28.5 37.5 2.0 39.5 72-73 29.04100829272429 34.0 27.5 36.5 2.0 39.0 74-75 28.32999514627602 34.0 26.0 36.0 2.0 39.0 76-77 27.551349328590437 33.5 25.5 35.5 2.0 38.0 78-79 26.746843577227956 32.5 23.5 35.0 2.0 37.0 80-81 25.976877045891392 32.0 21.0 35.0 2.0 36.5 82-83 25.208861684889833 31.5 19.0 35.0 2.0 36.0 84-85 24.430622792206535 31.0 13.0 34.0 2.0 35.5 86-87 23.65528144588899 31.0 4.5 34.0 2.0 35.0 88-89 22.746506420335166 30.0 2.0 34.0 2.0 35.0 90-91 21.81476700455796 29.0 2.0 34.0 2.0 35.0 92-93 20.833996964920328 29.0 2.0 34.0 2.0 35.0 94-95 19.717408779031377 26.5 2.0 33.5 2.0 35.0 96-97 18.41937944437879 25.0 2.0 33.0 2.0 35.0 98-99 16.84597471390068 11.0 2.0 32.0 2.0 34.0 100-101 13.008676281980666 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11357755140938508 1101 2 0.17174957069709507 1101 3 0.15391312561089876 1101 4 0.06385725909954232 1101 5 0.1395861790637838 1101 6 0.223750033378586 1101 7 0.18061841893018027 1101 8 0.2437618181142014 1101 9 0.19769675095805184 1101 10-11 0.24848328753892446 1101 12-13 0.2872534576848551 1101 14-15 0.3209409470819651 1101 16-17 0.4077184866481929 1101 18-19 0.44421449344959996 1101 20-21 0.3686914493877822 1101 22-23 0.2948314958721667 1101 24-25 0.47746818912792577 1101 26-27 0.6424527934190749 1101 28-29 0.542821302058293 1101 30-31 0.5486680172444096 1101 32-33 0.5573804290153532 1101 34-35 0.6610137447724895 1101 36-37 0.6431173701854433 1101 38-39 0.45705040839161626 1101 40-41 0.6252355987316776 1101 42-43 0.8822433679020847 1101 44-45 0.7886346512346094 1101 46-47 0.7112211605149454 1101 48-49 0.8950782603174474 1101 50-51 0.8839420340214375 1101 52-53 0.8625199148493579 1101 54-55 0.8011415522729379 1101 56-57 0.5078326031843616 1101 58-59 0.7989132913499049 1101 60-61 0.6913597783125383 1101 62-63 0.49391410115203627 1101 64-65 0.43333550272585697 1101 66-67 0.5326524896319498 1101 68-69 0.6516472925013908 1101 70-71 0.6497298127470259 1101 72-73 0.42496055341443295 1101 74-75 0.3322597922524402 1101 76-77 0.10981051496814587 1101 78-79 0.2498708553857405 1101 80-81 0.21277170981809235 1101 82-83 -0.29685060272240094 1101 84-85 -0.2089126109271895 1101 86-87 -0.210103545289126 1101 88-89 -0.18321548455945802 1101 90-91 -0.11881478330683848 1101 92-93 -0.2711639624267086 1101 94-95 -0.6401079418127011 1101 96-97 -0.5932461875383552 1101 98-99 -0.8033793194204151 1101 100-101 -1.0019376083558438 1102 1 0.19021398376010268 1102 2 0.18397877006914598 1102 3 0.12156008182927991 1102 4 0.12030356411727183 1102 5 0.057129059463811416 1102 6 0.21263194511764283 1102 7 0.29391512222687766 1102 8 0.11712422290517566 1102 9 0.11441458339017174 1102 10-11 0.17758207235199563 1102 12-13 0.19873929988498418 1102 14-15 0.16041097440913177 1102 16-17 0.11219025826282092 1102 18-19 0.17683179678119387 1102 20-21 0.12970313611375417 1102 22-23 0.29649554610764994 1102 24-25 0.1935287159027368 1102 26-27 0.1608797368460202 1102 28-29 0.16805300157570713 1102 30-31 0.15746678461460561 1102 32-33 0.1324513633719988 1102 34-35 0.21533783338229284 1102 36-37 0.15188415895222818 1102 38-39 0.09491510982775253 1102 40-41 0.02771103334997349 1102 42-43 0.13372136080865715 1102 44-45 0.2746297672297189 1102 46-47 0.22866141866948198 1102 48-49 0.23518815042733365 1102 50-51 0.039604281826548515 1102 52-53 0.22548840138927062 1102 54-55 0.2893583090611216 1102 56-57 0.07569119961438986 1102 58-59 0.0176216464868304 1102 60-61 0.1431223807894284 1102 62-63 0.05859867583661327 1102 64-65 0.03807298746334098 1102 66-67 -0.06783881800221536 1102 68-69 -0.16455859156163655 1102 70-71 -0.49211448052584217 1102 72-73 -0.5615813966989478 1102 74-75 -0.5474372831589172 1102 76-77 -0.5026172424596105 1102 78-79 -0.43746474552128944 1102 80-81 -0.28202944141163044 1102 82-83 -0.609033578476808 1102 84-85 -0.46916349760664744 1102 86-87 -0.3155558971700536 1102 88-89 -0.34769074474900563 1102 90-91 -0.5004375520724658 1102 92-93 -0.5605863125633412 1102 94-95 -0.70530475558094 1102 96-97 -0.8010510017717394 1102 98-99 -0.6524510677778785 1102 100-101 -0.8028521964132889 1103 1 0.22499671746644623 1103 2 0.2595651128725507 1103 3 0.2717590543712376 1103 4 0.17824474432828197 1103 5 0.20558021428971784 1103 6 0.2066221283647991 1103 7 0.29339260158731406 1103 8 0.298617481835997 1103 9 0.17739794954000843 1103 10-11 0.34414644661833194 1103 12-13 0.2332162622540963 1103 14-15 0.2874484593209985 1103 16-17 0.37750578055399586 1103 18-19 0.3892521953234436 1103 20-21 0.2903649624146212 1103 22-23 0.2788492776514673 1103 24-25 0.4395820262783374 1103 26-27 0.29004296842329325 1103 28-29 0.3128083596239932 1103 30-31 0.32513923439594805 1103 32-33 0.32476577592886713 1103 34-35 -0.028155125859434804 1103 36-37 -0.10629909484185163 1103 38-39 -0.15380913806046692 1103 40-41 0.016438963764599634 1103 42-43 0.19060699403454606 1103 44-45 0.3515331938039665 1103 46-47 0.36873593177148223 1103 48-49 -0.021839752174827254 1103 50-51 0.19815142009988307 1103 52-53 0.15401476601135755 1103 54-55 0.10367714396726768 1103 56-57 -0.08623075847226858 1103 58-59 0.21070131678460058 1103 60-61 0.03785603626088374 1103 62-63 0.05594156162134567 1103 64-65 -0.015090584719981592 1103 66-67 -0.2091543867396659 1103 68-69 -0.17896578498440263 1103 70-71 -0.08519337671895855 1103 72-73 -0.06308260679039535 1103 74-75 -0.2958922532148911 1103 76-77 -0.08506906412138093 1103 78-79 -0.006533680093411931 1103 80-81 -0.18621906166353241 1103 82-83 0.0881972280130654 1103 84-85 -0.12180974671020905 1103 86-87 -0.3103834957702496 1103 88-89 -0.201559415683203 1103 90-91 -0.3848026286728867 1103 92-93 -0.48837036611811513 1103 94-95 -0.5969353572648401 1103 96-97 -0.4985140396569747 1103 98-99 -0.4041257602509809 1103 100-101 -0.6871534093799845 1104 1 0.24654784843908573 1104 2 0.20155807902045098 1104 3 0.24460401887604633 1104 4 0.20746296032900347 1104 5 0.2508232588157213 1104 6 0.09525694231123794 1104 7 0.12418386338671183 1104 8 0.29333640194620614 1104 9 0.2503754583892359 1104 10-11 0.38343922269287845 1104 12-13 0.29482118698525994 1104 14-15 0.40193376614408294 1104 16-17 0.32879780337107434 1104 18-19 0.4868719844734244 1104 20-21 0.44866207282375115 1104 22-23 0.5146204198195079 1104 24-25 0.5188918644624891 1104 26-27 0.33319101559293784 1104 28-29 0.4331477083252935 1104 30-31 0.3980391411699884 1104 32-33 0.2777650443999704 1104 34-35 0.36946549104602155 1104 36-37 0.21498332601179015 1104 38-39 0.21667558519501284 1104 40-41 0.09875045021682638 1104 42-43 0.29224010383941845 1104 44-45 0.1327941550970806 1104 46-47 0.004126720301691478 1104 48-49 -0.0049108594736537725 1104 50-51 0.021888394591563554 1104 52-53 0.1407818014775799 1104 54-55 0.16038537775438755 1104 56-57 0.19501571710015497 1104 58-59 0.1963221696003643 1104 60-61 0.3416192712948103 1104 62-63 0.5011048312140574 1104 64-65 0.1469052992659492 1104 66-67 0.10507116007042328 1104 68-69 0.08717389461214964 1104 70-71 0.042671821233589924 1104 72-73 0.04659421678076825 1104 74-75 -0.17235776840472639 1104 76-77 -0.41992563996602783 1104 78-79 -0.33761853641058437 1104 80-81 -0.5003732145644548 1104 82-83 -0.253451081451594 1104 84-85 -0.3979556965544333 1104 86-87 -0.3823726128552245 1104 88-89 -0.4525866084850385 1104 90-91 -0.7921930881702934 1104 92-93 -1.2413766705107 1104 94-95 -1.188294210989067 1104 96-97 -1.3586215547454046 1104 98-99 -1.1014605945808995 1104 100-101 -1.3435076225001161 1105 1 0.14164384975192945 1105 2 0.28400413933287894 1105 3 0.16818001893856405 1105 4 0.22159844057000555 1105 5 0.25493467035150275 1105 6 0.17829104485330305 1105 7 0.1860816573492201 1105 8 0.23733172886642961 1105 9 0.2542670866996559 1105 10-11 0.3667801426175501 1105 12-13 0.3612557951264179 1105 14-15 0.29567639119585465 1105 16-17 0.3868918823133001 1105 18-19 0.4155362104066782 1105 20-21 0.4250099789245425 1105 22-23 0.4394936293188394 1105 24-25 0.27909908585549914 1105 26-27 0.3628788452677796 1105 28-29 0.32317404323976007 1105 30-31 0.22137201214453484 1105 32-33 0.20456062451047075 1105 34-35 0.17880061260079572 1105 36-37 0.2918254024846334 1105 38-39 0.30754764596068185 1105 40-41 0.20368863740571896 1105 42-43 0.24008017198198672 1105 44-45 0.3017498233553013 1105 46-47 0.35619991104618265 1105 48-49 0.3114509759995485 1105 50-51 0.46311542968654607 1105 52-53 0.388062838776257 1105 54-55 0.5154339449631209 1105 56-57 0.4672514302136861 1105 58-59 0.5453242560757801 1105 60-61 0.4924775003694961 1105 62-63 0.7547385805400566 1105 64-65 0.5658726892961958 1105 66-67 0.3218790089689705 1105 68-69 0.4642110409076814 1105 70-71 0.42171876302326794 1105 72-73 0.173703647337085 1105 74-75 0.06105494737355954 1105 76-77 0.38634472661285457 1105 78-79 0.4227161592531452 1105 80-81 0.275991004335733 1105 82-83 0.2672505447489115 1105 84-85 -0.012364587127784432 1105 86-87 0.0924729527984205 1105 88-89 -0.19270124741538908 1105 90-91 -0.22385515729748917 1105 92-93 -0.2862988965533546 1105 94-95 -0.6162458508649706 1105 96-97 -0.7230358177976015 1105 98-99 -0.6156434503171404 1105 100-101 -0.715238713328219 1106 1 0.33208243495558776 1106 2 0.3401602509259547 1106 3 0.3163312034008712 1106 4 0.37892054292315436 1106 5 0.2702883694353986 1106 6 0.2518757919175698 1106 7 0.33309082255960476 1106 8 0.2602976789475804 1106 9 0.44985591410895154 1106 10-11 0.4287867031315997 1106 12-13 0.5266376685153489 1106 14-15 0.5539852168617685 1106 16-17 0.06956151964825352 1106 18-19 0.39298898342243405 1106 20-21 0.4479019909123707 1106 22-23 0.5686938318832659 1106 24-25 0.44417482959489263 1106 26-27 0.3294564921996326 1106 28-29 0.29456493033084996 1106 30-31 0.3488072994498097 1106 32-33 0.32716399255924955 1106 34-35 0.1175842816736079 1106 36-37 0.3648222768490257 1106 38-39 0.49297452559672195 1106 40-41 0.43070141390823835 1106 42-43 0.2616856611460392 1106 44-45 0.3714301202284247 1106 46-47 0.2943648350222503 1106 48-49 0.41067382178076883 1106 50-51 0.35173923681863783 1106 52-53 0.26319696507185597 1106 54-55 0.23846340397495425 1106 56-57 0.11146781095180103 1106 58-59 0.31725144080375145 1106 60-61 0.5457725968989138 1106 62-63 0.360814060696633 1106 64-65 0.22174560502025642 1106 66-67 -0.05573969669411838 1106 68-69 -0.1775282839882344 1106 70-71 -0.3536661301530515 1106 72-73 -0.3495811445570176 1106 74-75 -0.27192849879535785 1106 76-77 -0.5757968941516616 1106 78-79 -0.8297422174917948 1106 80-81 -0.7642406579793963 1106 82-83 -0.6226782048723187 1106 84-85 -0.9950384272596189 1106 86-87 -0.9770789252810381 1106 88-89 -1.0374644587257897 1106 90-91 -1.0306167829600774 1106 92-93 -0.9804730417771097 1106 94-95 -1.4097455769048786 1106 96-97 -1.285605604980418 1106 98-99 -1.4936899591938655 1106 100-101 -1.111381635403177 1107 1 -0.06958201629563732 1107 2 -0.08069162447137757 1107 3 -0.11683067604199593 1107 4 0.06331053309826729 1107 5 -0.18391032443271627 1107 6 -0.17862758899903497 1107 7 -0.05782404769410476 1107 8 2.2717094319091302E-4 1107 9 0.03495676367261069 1107 10-11 -0.05323953509299173 1107 12-13 0.011611372497313255 1107 14-15 0.023239103471027533 1107 16-17 0.10808085155601788 1107 18-19 0.06353744322709787 1107 20-21 -0.03354631284998533 1107 22-23 0.12270180737884573 1107 24-25 0.018901755561486766 1107 26-27 -0.14840861789688375 1107 28-29 -0.13281760448970203 1107 30-31 -0.10154813024445986 1107 32-33 -0.09588403379456167 1107 34-35 -0.04137659216329581 1107 36-37 0.1771669569623029 1107 38-39 0.010861597202804774 1107 40-41 0.1314180526414077 1107 42-43 0.06284730941512606 1107 44-45 -0.15763998322184847 1107 46-47 -0.07658239956134594 1107 48-49 -0.1291747593901249 1107 50-51 0.0783762361829119 1107 52-53 -0.00794098788427533 1107 54-55 -0.02169379419877515 1107 56-57 -0.44626784182516843 1107 58-59 -0.24955778303026221 1107 60-61 -0.09067772963405929 1107 62-63 -0.2379549388988167 1107 64-65 -0.691101878075159 1107 66-67 -0.5111809496560333 1107 68-69 -0.47802530762575657 1107 70-71 -0.7896598884608608 1107 72-73 -0.588036903673931 1107 74-75 -0.6379054970037572 1107 76-77 -0.9162002924062982 1107 78-79 -0.9723764427706492 1107 80-81 -0.9692340117330822 1107 82-83 -1.0119459619086726 1107 84-85 -1.2603970355934315 1107 86-87 -1.1244233164270803 1107 88-89 -0.8725797566510032 1107 90-91 -0.7197747324486059 1107 92-93 -0.8404106248551884 1107 94-95 -0.9176921757605712 1107 96-97 -0.9555761303228429 1107 98-99 -1.010305574983036 1107 100-101 -0.7963495600405235 1108 1 -0.20735644609995418 1108 2 -0.1373672029402293 1108 3 -0.0703746414165991 1108 4 -0.1676764127081043 1108 5 -0.1750972197292029 1108 6 -0.10951177330787232 1108 7 -0.03084915459218962 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64-65 -0.3608309128395497 2316 66-67 -0.36804700673067003 2316 68-69 -0.37425882432526336 2316 70-71 -0.30188742284335035 2316 72-73 -0.5710970850858459 2316 74-75 -0.4116078845587161 2316 76-77 -0.33883590576277456 2316 78-79 -0.3166120769030094 2316 80-81 -0.2511069437052136 2316 82-83 -0.27206540191313877 2316 84-85 -0.369551197298847 2316 86-87 -0.34955406399258493 2316 88-89 -0.3413399733890401 2316 90-91 -0.3192826925660164 2316 92-93 -0.20659399126747857 2316 94-95 -0.28752217279716064 2316 96-97 -0.35653295952406694 2316 98-99 -0.6096194726639261 2316 100-101 -0.5106617269904259 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4999.0 3 1525.0 4 1500.0 5 1764.0 6 1965.0 7 2539.0 8 3052.0 9 3909.0 10 4876.0 11 6001.0 12 7400.0 13 8961.0 14 10535.0 15 12869.0 16 15007.0 17 18444.0 18 18039.0 19 16306.0 20 17239.0 21 18373.0 22 20163.0 23 22551.0 24 24970.0 25 28576.0 26 31473.0 27 36224.0 28 40992.0 29 47168.0 30 55122.0 31 64959.0 32 78191.0 33 95634.0 34 120936.0 35 156910.0 36 192122.0 37 202701.0 38 98701.0 39 5123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.85137253879394 18.54292990581409 14.401678457455876 23.204019097936094 2 15.49669378482314 19.74323810694382 40.52940858193128 24.23065952630176 3 19.583543126442223 23.630025924075955 29.477352708520975 27.309078240960844 4 12.988579206186643 16.554869814716273 36.51645733447069 33.940093644626394 5 14.11433467988738 36.80139565343325 33.930419344891085 15.153850321788287 6 32.33833807852036 35.519865564927 17.385379650640065 14.756416705912576 7 28.89687792358212 32.4248704178127 20.150623619500823 18.527628039104364 8 26.239368774093546 35.10539361424935 19.404116864982594 19.251120746674506 9 26.257433975734006 17.094484436194314 20.20958514120627 36.438496446865415 10-11 25.378623458999268 26.461321311748215 26.74301455882928 21.417040670423244 12-13 26.081815154639585 23.76332460071332 27.441690036120686 22.713170208526407 14-15 22.86043978892378 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28.887613062590972 22.29519815051945 40-41 22.44925634310324 26.426749403207367 28.8464191272916 22.277575126397792 42-43 22.360641391139367 26.3883252249228 28.68890926429744 22.56212411964039 44-45 22.34285735277076 26.580860658242354 28.582778219152967 22.493503769833914 46-47 22.485105717841275 26.878777008932897 28.17933390378818 22.456783369437648 48-49 22.542827525033932 26.78697380654676 28.126803404828316 22.543395263590995 50-51 22.433595199502477 27.04097463513139 28.022931019567988 22.502499145798144 52-53 22.724965690415686 26.96185709181612 27.78351737517489 22.529659842593304 54-55 22.410957055718786 26.864999671018463 28.020643340929414 22.70339993233334 56-57 22.458535614577137 27.18285935049397 27.925146228511494 22.43345880641739 58-59 22.67587048085231 26.764349202988125 27.980365522969848 22.57941479318972 60-61 22.521156067511072 26.859498198549286 28.230764684018965 22.38858104992067 62-63 22.388693783284154 26.979381150627653 28.165177907922402 22.466747158165795 64-65 22.212641470997816 26.857064616638226 28.06467341029845 22.8656205020655 66-67 22.231769402752057 27.047655940837906 27.903395742506643 22.817178913903394 68-69 22.320469413059975 26.88679812512693 27.993089478691225 22.799642983121867 70-71 22.30161187377947 26.901268597869205 27.901972316825162 22.89514721152616 72-73 22.364301123104653 26.864735475738875 27.861759300172906 22.909204100983562 74-75 22.31874794196835 27.076649948603475 27.651362592281636 22.953239517146535 76-77 22.415986419997118 26.88384476486081 27.65236100797598 23.047807807166095 78-79 22.437090895492286 27.01194942429872 27.564812304421167 22.986147375787823 80-81 22.41049319232711 27.157466001704258 27.519386574847005 22.91265423112163 82-83 22.550241587670563 27.036850675747644 27.49628628447812 22.916621452103673 84-85 22.578413499742904 26.850805670746773 27.504257066730776 23.066523762779546 86-87 22.552751738357387 26.81022062322095 27.597953204170388 23.039074434251276 88-89 22.497324431249574 26.806059010821187 27.458182964810756 23.238433593118476 90-91 22.56827191737493 26.86477684033165 27.43304808934875 23.13390315294467 92-93 22.716744524947558 26.513976906108024 27.484624618183723 23.284653950760692 94-95 22.530304741122713 26.533443619451425 27.48446094875905 23.451790690666815 96-97 22.66504770048903 26.4583389006173 27.420365570134415 23.456247828759253 98-99 22.57482713957156 26.387576092990262 27.20974444511986 23.827852322318314 100-101 23.41082871953119 25.32429201924761 27.210647420838917 24.05423184038228 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1114.0 1 728.5 2 497.0 3 609.5 4 458.5 5 426.0 6 818.0 7 1734.5 8 2729.5 9 3093.5 10 2497.5 11 1788.0 12 1642.5 13 1699.5 14 1826.5 15 1922.5 16 2013.0 17 2078.5 18 2136.0 19 2341.5 20 2589.0 21 2985.5 22 3575.0 23 4317.0 24 5261.5 25 6449.5 26 8006.0 27 9953.0 28 11662.0 29 13369.5 30 15688.5 31 17982.5 32 20356.0 33 22710.5 34 25023.0 35 27367.5 36 30373.0 37 33695.5 38 36661.0 39 38807.5 40 40492.5 41 43572.0 42 46850.5 43 49750.5 44 52390.0 45 54783.0 46 56409.5 47 58068.0 48 61020.5 49 61889.5 50 60533.0 51 58515.0 52 56008.5 53 54012.5 54 52218.5 55 48545.0 56 43550.5 57 39329.5 58 35737.0 59 31511.0 60 26294.5 61 21296.5 62 17061.0 63 13460.5 64 10515.0 65 8299.0 66 6494.5 67 5221.5 68 4099.0 69 2941.5 70 2146.0 71 1616.0 72 1280.5 73 1001.0 74 754.5 75 600.5 76 425.0 77 274.5 78 158.0 79 95.5 80 52.0 81 33.5 82 23.5 83 14.5 84 10.0 85 7.5 86 6.0 87 4.0 88 3.0 89 4.0 90 3.0 91 1.0 92 1.0 93 1.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1442096808759937 2 0.052743355505571764 3 0.15342307715418219 4 0.13025605897641837 5 0.19308073939508044 6 0.1983550749456376 7 0.16263647343237067 8 0.07003516446246176 9 0.12124295392166877 10-11 0.10084663100147614 12-13 0.15202103859011 14-15 0.13726625179677918 16-17 0.13339395481029417 18-19 0.13265955365768495 20-21 0.1307567870350156 22-23 0.19331441248909248 24-25 0.2132100073506879 26-27 0.1469469942629917 28-29 0.12635038011936023 30-31 0.0968074246621254 32-33 0.12197735507427801 34-35 0.09153308911156822 36-37 0.08435598693834169 38-39 0.05007280585972003 40-41 0.05731667177409286 42-43 0.061188968760577875 44-45 0.04035868152293434 46-47 0.05127455320035331 48-49 0.04346319548623699 50-51 0.053911720975631905 52-53 0.02830782624602839 54-55 0.051708517517804226 56-57 0.028007389410870068 58-59 0.05064029765946352 60-61 0.03695373072447339 62-63 0.051174407588633876 64-65 0.0403252996523612 66-67 0.038489296770838136 68-69 0.06482759265305087 70-71 0.051574990035511636 72-73 0.05097411636519499 74-75 0.042461739369042587 76-77 0.021564688390252763 78-79 0.02276643573088604 80-81 0.026104622788200714 82-83 0.021998652707703667 84-85 0.020629996014204652 86-87 0.02380127371865359 88-89 0.06282468041866207 90-91 0.10779006008069066 92-93 0.07941547009351596 94-95 0.07384069770780047 96-97 0.06536170258222122 98-99 0.053544520399327285 100-101 0.12985547652954063 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1497819.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.68778472145765 #Duplication Level Percentage of deduplicated Percentage of total 1 82.91317761326356 53.63469784018774 2 10.346565120416878 13.385927542321388 3 2.8773506637210384 5.583883209087893 4 1.2485975931299589 3.230760492324837 5 0.6450628405758345 2.0863843081490767 6 0.3925703826421243 1.5236705040224392 7 0.28712583352299764 1.300147387483333 8 0.20598751558584716 1.0659900850820145 9 0.1528793833675173 0.8900485775664435 >10 0.8420500116908943 9.798919407983215 >50 0.059772225818449654 2.583021460704453 >100 0.027102353313968333 3.171273166228793 >500 0.0011378289682277645 0.4572793126894209 >1k 4.137559884464598E-4 0.4669146976802427 >5k 2.068779942232299E-4 0.8210820084887158 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6602 0.4407742190478289 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5669 0.37848364855833716 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3404 0.22726377486198263 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 6.676374114629338E-5 0.0 0.0 0.0 0.0 18-19 6.676374114629338E-5 0.0 0.0 0.0 0.0 20-21 6.676374114629338E-5 0.0 0.0 3.338187057314669E-5 0.0 22-23 6.676374114629338E-5 0.0 0.0 3.0043683515832017E-4 3.338187057314669E-5 24-25 6.676374114629338E-5 0.0 0.0 5.674917997434937E-4 6.676374114629338E-5 26-27 6.676374114629338E-5 0.0 0.0 9.346923760481072E-4 1.0014561171944007E-4 28-29 6.676374114629338E-5 0.0 0.0 0.0030377502221563485 1.3352748229258677E-4 30-31 6.676374114629338E-5 0.0 0.0 0.009914415560224566 1.3352748229258677E-4 32-33 6.676374114629338E-5 0.0 0.0 0.024735966094701696 1.3352748229258677E-4 34-35 6.676374114629338E-5 0.0 0.0 0.0479029842724655 1.3352748229258677E-4 36-37 6.676374114629338E-5 0.0 0.0 0.0856578798906944 1.3352748229258677E-4 38-39 6.676374114629338E-5 0.0 0.0 0.13836785352569303 1.3352748229258677E-4 40-41 6.676374114629338E-5 0.0 0.0 0.1949835060177498 1.3352748229258677E-4 42-43 6.676374114629338E-5 0.0 0.0 0.2538023619676343 1.6690935286573346E-4 44-45 6.676374114629338E-5 0.0 0.0 0.32770982341658106 2.0029122343888012E-4 46-47 6.676374114629338E-5 0.0 0.0 0.4128669752486782 2.0029122343888012E-4 48-49 6.676374114629338E-5 0.0 0.0 0.4996264568682865 2.0029122343888012E-4 50-51 6.676374114629338E-5 0.0 0.0 0.5915601284267324 2.0029122343888012E-4 52-53 6.676374114629338E-5 0.0 0.0 0.6835271818557516 2.0029122343888012E-4 54-55 6.676374114629338E-5 0.0 0.0 0.7833723567400334 2.0029122343888012E-4 56-57 6.676374114629338E-5 0.0 0.0 0.8992074476288523 2.0029122343888012E-4 58-59 6.676374114629338E-5 0.0 0.0 1.026091937677383 2.0029122343888012E-4 60-61 6.676374114629338E-5 0.0 0.0 1.162223205874675 2.0029122343888012E-4 62-63 6.676374114629338E-5 0.0 0.0 1.2890743140526326 2.0029122343888012E-4 64-65 6.676374114629338E-5 0.0 0.0 1.4183956806530027 2.0029122343888012E-4 66-67 6.676374114629338E-5 0.0 0.0 1.5733877057241228 2.0029122343888012E-4 68-69 6.676374114629338E-5 0.0 0.0 1.7430677538474275 2.0029122343888012E-4 70-71 6.676374114629338E-5 0.0 0.0 1.9217275251549086 2.0029122343888012E-4 72-73 6.676374114629338E-5 0.0 0.0 2.099052021639464 2.6705496458517353E-4 74-75 6.676374114629338E-5 0.0 0.0 2.2751080070422396 2.6705496458517353E-4 76-77 6.676374114629338E-5 0.0 0.0 2.4681219826961738 2.6705496458517353E-4 78-79 6.676374114629338E-5 0.0 0.0 2.6785279129187174 2.6705496458517353E-4 80-81 6.676374114629338E-5 0.0 0.0 2.9087292923911363 2.6705496458517353E-4 82-83 6.676374114629338E-5 0.0 0.0 3.140299328557055 3.338187057314669E-4 84-85 6.676374114629338E-5 0.0 0.0 3.369165433206549 3.338187057314669E-4 86-87 6.676374114629338E-5 0.0 0.0 3.6109503217678505 3.338187057314669E-4 88-89 6.676374114629338E-5 0.0 0.0 3.864118428194595 3.338187057314669E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3850 0.0 44.408104 1 GTATCAA 6055 0.0 35.23647 1 ATCAACG 7450 0.0 28.152332 3 TATCAAC 7655 0.0 27.646927 2 TCAACGC 7610 0.0 27.56043 4 CAACGCA 7690 0.0 27.33556 5 AACGCAG 7870 0.0 26.645462 6 GTACATG 5345 0.0 24.235373 1 ACGCAGA 8765 0.0 23.757154 7 GTGTAGC 805 0.0 23.664244 1 TACATGG 5310 0.0 23.465986 2 CGCAGAG 8935 0.0 23.09231 8 ACATGGG 5450 0.0 22.42687 3 GAGTACT 5260 0.0 22.277903 12-13 GCAGAGT 9505 0.0 21.106585 9 CCTATAC 385 0.0 21.000048 3 GTACTTT 5665 0.0 20.685566 14-15 TGGTATC 2065 0.0 20.267242 2 AGAGTAC 8180 0.0 20.02136 10-11 AGTACTT 5545 0.0 19.846895 12-13 >>END_MODULE