FastQCFastQC Report
Thu 26 May 2016
SRR938196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497819
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64180.42848969067691095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57990.38716293490735526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34730.23187047300107688No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15610.10421819992936397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40800.042.268031
GTATCAA61600.032.1603551
GTATTCG1004.9485105E-528.5047191
ATCAACG73200.026.6007143
TATCAAC74300.026.5930712
TCAACGC74100.026.2758754
CAACGCA75550.025.771575
AACGCAG77400.025.1555826
GTACATG56550.023.1868611
GAGTACT50200.023.13106712-13
TACATGG55700.022.5972022
ACGCAGA87550.022.1307187
CGCAGAG88850.021.8069158
ACATGGG56200.021.4643573
GTACTTT53900.021.41105314-15
GTATTAG4100.020.8571111
CATGGGG34800.020.4693244
GCAGAGT94800.020.287959
GTATAGC5550.019.6879431
GTACCTG11250.019.4254381
Produced by FastQC (version 0.11.5)