##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938196_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1497819 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50298734359759 34.0 31.0 34.0 31.0 34.0 2 32.71905417143193 34.0 31.0 34.0 31.0 34.0 3 32.77553496116687 34.0 31.0 34.0 31.0 34.0 4 36.23233047517758 37.0 35.0 37.0 35.0 37.0 5 36.20341109306265 37.0 35.0 37.0 35.0 37.0 6 36.23240792111731 37.0 37.0 37.0 35.0 37.0 7 36.1934078817267 37.0 37.0 37.0 35.0 37.0 8 36.18228637772655 37.0 37.0 37.0 35.0 37.0 9 37.86519933316375 39.0 38.0 39.0 35.0 39.0 10-11 37.841151033602856 39.0 38.0 39.0 35.0 39.0 12-13 37.75441658838618 39.0 38.0 39.0 35.0 39.0 14-15 39.030343118894876 40.0 38.0 41.0 36.0 41.0 16-17 38.91308395740741 40.0 38.0 41.0 35.0 41.0 18-19 39.158774191007055 40.0 38.5 41.0 36.0 41.0 20-21 39.14149707007322 40.0 39.0 41.0 36.0 41.0 22-23 39.043911180189326 40.0 39.0 41.0 35.0 41.0 24-25 38.94769594991117 40.0 38.0 41.0 35.0 41.0 26-27 38.78201504988253 40.0 38.0 41.0 35.0 41.0 28-29 38.65993120664112 40.0 38.0 41.0 34.5 41.0 30-31 38.43329100512145 40.0 38.0 41.0 34.0 41.0 32-33 38.23545067862005 40.0 38.0 41.0 34.0 41.0 34-35 38.08944505310721 40.0 38.0 41.0 34.0 41.0 36-37 38.100601941890176 40.0 38.0 41.0 34.0 41.0 38-39 37.968623712210885 40.0 38.0 41.0 33.0 41.0 40-41 37.79644836926224 40.0 37.0 41.0 33.0 41.0 42-43 37.575267772674806 40.0 37.0 41.0 33.0 41.0 44-45 37.30574989367874 40.0 37.0 41.0 32.0 41.0 46-47 36.92223058994445 40.0 36.0 41.0 31.0 41.0 48-49 36.547300107689914 39.0 36.0 41.0 30.0 41.0 50-51 36.18069840214338 39.0 35.0 40.5 29.5 41.0 52-53 35.66267319349 38.5 35.0 40.0 28.0 41.0 54-55 35.3486552781077 38.0 35.0 40.0 27.5 41.0 56-57 35.005405526301914 38.0 34.0 40.0 27.0 41.0 58-59 34.56985456854267 38.0 34.0 40.0 26.0 41.0 60-61 34.088151505622506 37.0 33.0 40.0 25.0 41.0 62-63 33.61354709747974 36.5 33.0 39.5 23.5 41.0 64-65 33.10777470442023 36.0 32.0 39.0 22.0 40.0 66-67 32.53942599205912 35.5 31.5 39.0 20.5 40.0 68-69 31.95321731130397 35.0 31.0 38.0 18.5 40.0 70-71 31.323094779809843 35.0 30.5 37.5 15.0 39.5 72-73 30.718511716035117 34.5 29.5 37.0 10.0 39.0 74-75 30.098994938640786 34.0 29.0 36.0 7.5 39.0 76-77 28.75357603288515 32.5 27.0 35.0 7.0 37.0 78-79 29.43333840737766 34.0 29.0 35.0 2.0 37.0 80-81 29.075313505837485 34.0 29.0 35.0 2.0 37.0 82-83 28.487602640906545 34.0 27.5 35.0 2.0 36.5 84-85 27.86528579220854 33.0 26.5 35.0 2.0 36.0 86-87 27.184726592465445 33.0 25.5 35.0 2.0 35.5 88-89 26.416745948609275 32.0 24.5 35.0 2.0 35.0 90-91 25.846865008388864 32.0 23.5 34.0 2.0 35.0 92-93 24.996167761258203 31.0 19.0 34.0 2.0 35.0 94-95 24.135011306439566 31.0 12.0 34.0 2.0 35.0 96-97 23.053008407557922 31.0 2.0 34.0 2.0 35.0 98-99 21.573112639110597 30.0 2.0 34.0 2.0 35.0 100-101 17.240780761894463 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1503250017006934 1101 2 0.1387061791278157 1101 3 0.12287654565439965 1101 4 0.10519423768469238 1101 5 0.1673427899499984 1101 6 0.10128950232541456 1101 7 0.10088967486415612 1101 8 0.05399082351083706 1101 9 0.027558237504464955 1101 10-11 0.07623402860379969 1101 12-13 0.12496957944297549 1101 14-15 0.14759866040648006 1101 16-17 0.2455832609817179 1101 18-19 0.25244820411688806 1101 20-21 0.22692806993118353 1101 22-23 0.33403752231793504 1101 24-25 0.37288551127658565 1101 26-27 0.30227391268416426 1101 28-29 0.40707094969030067 1101 30-31 0.3761722509343244 1101 32-33 0.324826456310241 1101 34-35 0.2667515696536924 1101 36-37 0.27147659800505153 1101 38-39 0.2645185895145872 1101 40-41 0.2438939550061079 1101 42-43 0.22158333923743356 1101 44-45 0.4148910682439251 1101 46-47 0.3636752113158579 1101 48-49 0.2544160340853381 1101 50-51 0.14369497454065794 1101 52-53 -0.05749554538836321 1101 54-55 -0.25373326919255845 1101 56-57 0.14593832806795604 1101 58-59 0.2769685569018847 1101 60-61 0.30592089426490787 1101 62-63 0.3530438009938237 1101 64-65 0.2770882878934344 1101 66-67 0.05978586532636854 1101 68-69 0.06210851770757486 1101 70-71 0.12624079706813873 1101 72-73 -0.10769945465978026 1101 74-75 -0.35140871660943773 1101 76-77 -0.5364271799060596 1101 78-79 -0.4530289063408013 1101 80-81 -0.6399627020960814 1101 82-83 -0.7007916229831501 1101 84-85 -0.6022796701398256 1101 86-87 -0.7629478103087983 1101 88-89 -1.0175526517117532 1101 90-91 -1.3033108148718533 1101 92-93 -1.3509854639743608 1101 94-95 -0.9906224958019152 1101 96-97 -1.0058399095354744 1101 98-99 -0.857520705850213 1101 100-101 -1.1491073289740399 1102 1 0.08437581860865606 1102 2 0.12139412610147815 1102 3 0.16989962839176798 1102 4 0.07663443769632039 1102 5 0.07610725300018117 1102 6 0.03716426248588789 1102 7 0.04191473446064009 1102 8 0.10569848093278011 1102 9 0.04441386864581176 1102 10-11 0.18310041618447315 1102 12-13 0.08534983410894625 1102 14-15 0.10973337754119683 1102 16-17 0.18856395753384447 1102 18-19 0.16057076938231063 1102 20-21 0.22084690242144234 1102 22-23 0.2741767746000505 1102 24-25 0.19152525777347051 1102 26-27 0.1457985362087868 1102 28-29 0.18661510923445945 1102 30-31 0.21333720238498444 1102 32-33 -0.04367723576487492 1102 34-35 0.1837266844859542 1102 36-37 0.05146438799284425 1102 38-39 -0.0344749566218141 1102 40-41 0.08545858085645364 1102 42-43 0.2140369702624909 1102 44-45 0.023254635893202646 1102 46-47 -0.03661637540430007 1102 48-49 0.1322435240556814 1102 50-51 0.17781788866357573 1102 52-53 0.2321727492799326 1102 54-55 0.1260359034337668 1102 56-57 -0.014553270280785569 1102 58-59 0.22534520670709668 1102 60-61 0.06035341941171879 1102 62-63 0.06261703199562874 1102 64-65 0.2510707868759354 1102 66-67 -0.16248577837384204 1102 68-69 0.2650397927817103 1102 70-71 0.12211352222658078 1102 72-73 -0.19140461193636682 1102 74-75 -0.2605935529371308 1102 76-77 -0.3167283602072395 1102 78-79 -0.5456246967937375 1102 80-81 -0.43421905420957785 1102 82-83 -0.136447765782151 1102 84-85 -0.4080320394636239 1102 86-87 -0.4820096743706621 1102 88-89 -0.5865601521478254 1102 90-91 -0.8682102276284098 1102 92-93 -0.9635234022265848 1102 94-95 -0.9516074367868512 1102 96-97 -1.1005855342810946 1102 98-99 -0.78801986277794 1102 100-101 -1.096042034480174 1103 1 0.07832342609999898 1103 2 0.08897628210457498 1103 3 0.07857684389310293 1103 4 -0.00429875651934708 1103 5 0.029554258072224115 1103 6 -0.03026246436188984 1103 7 0.03544653716790691 1103 8 0.0362248002144554 1103 9 0.12682670860907308 1103 10-11 0.1914416702671886 1103 12-13 0.0503716389781772 1103 14-15 0.08985824062143877 1103 16-17 0.182329926898106 1103 18-19 0.12821208909803516 1103 20-21 0.11581389515205132 1103 22-23 0.12604106741950716 1103 24-25 0.0428402127565235 1103 26-27 0.11143603074167885 1103 28-29 0.08787225518802444 1103 30-31 0.0958897682377895 1103 32-33 0.12405244246746605 1103 34-35 0.13741697959122945 1103 36-37 0.1800799405293887 1103 38-39 0.05553710282278246 1103 40-41 0.03014852706170501 1103 42-43 -0.06393295134835597 1103 44-45 -0.08029181397992602 1103 46-47 -0.0749796195884258 1103 48-49 -0.07714127839004448 1103 50-51 -0.1733372690021966 1103 52-53 -0.14130371600341363 1103 54-55 -0.10960238179173842 1103 56-57 -0.1884860149628338 1103 58-59 -0.3087458092001967 1103 60-61 -0.36184878667594234 1103 62-63 -0.3962077886747366 1103 64-65 -0.33479483629766094 1103 66-67 -0.4709186532713474 1103 68-69 -0.37536067367163994 1103 70-71 -0.3579787178719336 1103 72-73 -0.6610281924910772 1103 74-75 -0.7782575152139053 1103 76-77 -0.4932726022233176 1103 78-79 -0.3615650044782157 1103 80-81 -0.2533235146558681 1103 82-83 -0.42422586978974763 1103 84-85 -0.8205518551603639 1103 86-87 -0.975102725620264 1103 88-89 -1.005113284202892 1103 90-91 -1.2030038540930583 1103 92-93 -1.0717473794546386 1103 94-95 -1.1118548406333773 1103 96-97 -1.211617299665459 1103 98-99 -1.2635470971619007 1103 100-101 -1.2690538710932238 1104 1 0.22557905457851035 1104 2 0.11773457647304753 1104 3 0.11506999576864274 1104 4 0.1281188378370146 1104 5 0.11490671117729079 1104 6 0.129685759533551 1104 7 0.03949374406228401 1104 8 -0.019354840725910094 1104 9 0.07239557838141053 1104 10-11 0.11395625687058697 1104 12-13 0.18176100583836785 1104 14-15 0.17037039362375594 1104 16-17 0.19607015033000152 1104 18-19 0.2681102648284508 1104 20-21 0.23198083894434518 1104 22-23 0.17767205610098813 1104 24-25 0.22445933349396796 1104 26-27 0.20079094021109256 1104 28-29 0.10102916968812536 1104 30-31 -0.11734314456126782 1104 32-33 0.10199379392312835 1104 34-35 0.19578486309292487 1104 36-37 0.11377776218740365 1104 38-39 0.1629779519343515 1104 40-41 0.11095194651954898 1104 42-43 -0.013631544888347946 1104 44-45 -0.004083472067257787 1104 46-47 -0.1296916693282526 1104 48-49 -0.13160888159303852 1104 50-51 -0.02245451952963151 1104 52-53 -0.1672719570954726 1104 54-55 -0.1615942201228151 1104 56-57 -0.008931109425247996 1104 58-59 -0.04737917457456575 1104 60-61 -0.16757328482332667 1104 62-63 -0.23018435698680406 1104 64-65 -0.2955398265663618 1104 66-67 -0.3844965848966737 1104 68-69 -0.21447346466648654 1104 70-71 -0.3276668299583001 1104 72-73 -0.380363473808945 1104 74-75 -0.5186516716741743 1104 76-77 -0.7008108160718756 1104 78-79 -0.665546042669817 1104 80-81 -0.5986162762447051 1104 82-83 -0.5077386363658043 1104 84-85 -0.6335564626542443 1104 86-87 -0.48686849358592 1104 88-89 -0.738229400705336 1104 90-91 -0.8207838863548282 1104 92-93 -0.9151634641919664 1104 94-95 -0.9798043040926316 1104 96-97 -0.9302518342444301 1104 98-99 -1.118797498364632 1104 100-101 -1.4986579763464682 1105 1 0.10050774713205612 1105 2 0.05785195048353131 1105 3 0.12994199401818918 1105 4 0.020185737897186584 1105 5 0.05614644485862641 1105 6 0.05540977431861194 1105 7 -0.002837481956923682 1105 8 -0.010909303985975782 1105 9 0.13860796126137132 1105 10-11 0.09116815525063515 1105 12-13 0.11883804523133534 1105 14-15 0.03547477600358917 1105 16-17 -0.002228043735662766 1105 18-19 0.08050281525160585 1105 20-21 0.053248724414821424 1105 22-23 0.1020241351526181 1105 24-25 -0.002716911162856661 1105 26-27 0.16762027902500876 1105 28-29 0.18490137892957392 1105 30-31 0.14425554885188063 1105 32-33 0.23722445886018306 1105 34-35 0.2062001459392846 1105 36-37 0.14067336808579967 1105 38-39 0.2106171870496496 1105 40-41 0.19495749091770875 1105 42-43 0.2118064586594457 1105 44-45 0.22893802956988196 1105 46-47 0.2555292274654519 1105 48-49 0.3123410234606041 1105 50-51 0.15851010416241706 1105 52-53 0.19401210442038774 1105 54-55 0.37057081070545195 1105 56-57 0.40377831956816124 1105 58-59 0.47006672944756644 1105 60-61 0.33534397118798864 1105 62-63 0.40843585369250945 1105 64-65 0.37437898062611197 1105 66-67 0.39161756953377136 1105 68-69 0.6240156575290747 1105 70-71 0.28678797088879193 1105 72-73 0.5023982928965331 1105 74-75 0.47913633226433916 1105 76-77 0.5488150640378429 1105 78-79 0.4853380719847422 1105 80-81 0.3013335154984773 1105 82-83 0.5953675355378856 1105 84-85 0.2914857357250291 1105 86-87 0.22592171795299265 1105 88-89 0.15699260965671158 1105 90-91 0.1571874851706454 1105 92-93 -0.13636795441210126 1105 94-95 0.0380919113379079 1105 96-97 -0.05510042802250936 1105 98-99 -0.30770557622845374 1105 100-101 -0.9045301934024295 1106 1 0.2513352311406791 1106 2 0.21396769447197528 1106 3 0.23706194702988626 1106 4 0.21451632056049874 1106 5 0.16904654074465952 1106 6 0.1527065976268034 1106 7 0.19971242236458409 1106 8 0.14299504243241756 1106 9 0.15685951473962945 1106 10-11 0.23178595601523 1106 12-13 0.1129346722014617 1106 14-15 0.16711752996667428 1106 16-17 0.23708906922306028 1106 18-19 0.3088892836984556 1106 20-21 0.3741724784152609 1106 22-23 0.4240297202027037 1106 24-25 0.3931504942275126 1106 26-27 0.42119317523979305 1106 28-29 0.4669946623412855 1106 30-31 0.40433499727888744 1106 32-33 0.47278837042917843 1106 34-35 0.508116361886259 1106 36-37 0.5019551277397838 1106 38-39 0.4815139082454465 1106 40-41 0.39515616387161856 1106 42-43 0.430383547293836 1106 44-45 0.19243051227096686 1106 46-47 0.3613834122058748 1106 48-49 0.29777470021259944 1106 50-51 0.14963960841917157 1106 52-53 0.05690571450876547 1106 54-55 -0.0072702281014116465 1106 56-57 -0.2655287585644217 1106 58-59 -0.19461960700033387 1106 60-61 -0.27909066442433783 1106 62-63 -0.14593614468033422 1106 64-65 -0.12989749491879365 1106 66-67 -0.5032927915539389 1106 68-69 -0.5717783805181682 1106 70-71 -0.7533780551540197 1106 72-73 -0.8917291026976919 1106 74-75 -0.8495760864296997 1106 76-77 -0.7309194644809871 1106 78-79 -0.6146965362315697 1106 80-81 -0.7666028800998959 1106 82-83 -0.734684589520743 1106 84-85 -0.9847910843454564 1106 86-87 -0.9910334024026568 1106 88-89 -1.028731060080247 1106 90-91 -1.1791060526918855 1106 92-93 -1.034617204135028 1106 94-95 -1.2341097523470168 1106 96-97 -1.6510985352073213 1106 98-99 -1.886824005855992 1106 100-101 -1.9898002723942554 1107 1 -0.020698520231917428 1107 2 0.01712321663576688 1107 3 -0.03144640412309485 1107 4 -0.04572756778256348 1107 5 0.026643489250702146 1107 6 -0.008710497674584872 1107 7 0.042167487960149685 1107 8 -0.04922595970594301 1107 9 -0.09049643509565897 1107 10-11 -0.01380729371025069 1107 12-13 -0.05706474986408239 1107 14-15 -0.012995745187922125 1107 16-17 -0.11247459025794626 1107 18-19 -0.038333741210514916 1107 20-21 -0.002843068021768147 1107 22-23 -0.03895357749134831 1107 24-25 0.03734514255439336 1107 26-27 0.051199532518872104 1107 28-29 0.07729345445826397 1107 30-31 -0.05656773635111989 1107 32-33 -0.12887030147742706 1107 34-35 -0.13313082068324178 1107 36-37 -0.2235837961462508 1107 38-39 -0.2342290265057585 1107 40-41 -0.16177038065822558 1107 42-43 -0.03863916553053315 1107 44-45 -0.14670143016676462 1107 46-47 -0.12367698194542953 1107 48-49 -0.1490264325389461 1107 50-51 -0.23217279405438518 1107 52-53 -0.3638305739961183 1107 54-55 -0.22318654319128228 1107 56-57 -0.4131621169415851 1107 58-59 -0.13465890654376977 1107 60-61 -0.32338934095441374 1107 62-63 -0.4116415136914853 1107 64-65 -0.5355785906825403 1107 66-67 -0.47228660765519237 1107 68-69 -0.43324748992116113 1107 70-71 -0.4990579950488332 1107 72-73 -0.6782112156260851 1107 74-75 -0.661574005865635 1107 76-77 -0.6904640521247494 1107 78-79 -0.5963760137788157 1107 80-81 -0.5472767516828583 1107 82-83 -0.5005055454243426 1107 84-85 -0.5912466122885895 1107 86-87 -0.829009475915921 1107 88-89 -0.6550097400779329 1107 90-91 -1.0671601473575514 1107 92-93 -1.0576478924549697 1107 94-95 -1.235988039349273 1107 96-97 -1.4888723316588042 1107 98-99 -2.071242892754217 1107 100-101 -2.3750513849180948 1108 1 -0.005902606307735425 1108 2 -0.04548291944484362 1108 3 -0.10413274642338166 1108 4 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2316 58-59 0.0018176809172061326 2316 60-61 0.21261733436650587 2316 62-63 0.11360296762462241 2316 64-65 0.28101515285835177 2316 66-67 0.24511366008338342 2316 68-69 0.42497860509081065 2316 70-71 0.4706312666992396 2316 72-73 0.6912758369763985 2316 74-75 1.009258925764975 2316 76-77 0.7125246516982635 2316 78-79 0.643962398381337 2316 80-81 0.7821398763551244 2316 82-83 1.0209745515250503 2316 84-85 1.289144934913768 2316 86-87 1.4303497231594093 2316 88-89 1.6880846432820036 2316 90-91 1.7562800335634279 2316 92-93 2.0637293567393407 2316 94-95 2.601210830357097 2316 96-97 2.9324292136189314 2316 98-99 3.387983361990173 2316 100-101 4.00924702512178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 13.0 5 30.0 6 52.0 7 130.0 8 301.0 9 609.0 10 1010.0 11 1829.0 12 2784.0 13 4185.0 14 5712.0 15 7951.0 16 10444.0 17 14222.0 18 14993.0 19 12029.0 20 11750.0 21 12644.0 22 13554.0 23 15145.0 24 16879.0 25 19261.0 26 22412.0 27 26296.0 28 31050.0 29 37881.0 30 45012.0 31 54663.0 32 67533.0 33 86806.0 34 116039.0 35 163221.0 36 228412.0 37 287811.0 38 157843.0 39 7309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94241720733371 19.008444354528013 14.888758465553883 23.16037997258439 2 15.722655206675142 19.964864157434214 40.016866806978555 24.295613828912085 3 19.58712086309607 23.681458898374682 29.679343623929842 27.0520766145994 4 13.169949106000123 16.874535574725652 36.3194751835836 33.63604013569063 5 13.981583729802125 36.93916301577107 34.03130979579465 15.04794345863216 6 32.44142222400262 35.77352040833358 17.118831348749328 14.666226018914472 7 28.966317024954286 32.367996400099074 20.093015244165016 18.572671330781624 8 26.20289901516805 35.43705881685304 19.275426470087506 19.0846156978914 9 25.97009384979093 17.08243786465521 20.21919871493151 36.72826957062235 10-11 25.413517921724853 26.545363625377966 26.65392146848184 21.38719698441534 12-13 25.94585013012366 23.711481994330374 27.572696330499408 22.769971545046555 14-15 22.680711087254203 25.319113991743997 25.88797444818099 26.112200472820813 16-17 22.262811856861727 28.609928344068646 26.58854005838631 22.538719740683312 18-19 22.08143974672507 27.027497982065924 29.027973339902886 21.86308893130612 20-21 22.708351276088766 26.11650673412475 29.23834588825486 21.936796101531627 22-23 22.49247071909223 26.05114503154253 29.157628525208988 22.29875572415626 24-25 22.39070592233392 26.258386629197656 29.222391139900562 22.128516308567868 26-27 22.09486930104581 26.578308691327702 29.109251845270705 22.217570162355777 28-29 21.986986886734503 26.766384905055684 29.19460913922495 22.05201906898486 30-31 22.24559402871672 26.39459969948627 29.016133000527116 22.343673271269893 32-33 21.983096756016582 26.48804695360387 28.989116842555738 22.539739447823802 34-35 22.542231023957314 26.592616802804596 28.77408426140485 22.091067911833235 36-37 22.44632741769058 26.383946946488816 28.842639095718848 22.327086540101757 38-39 22.17221487801947 26.58898503117948 28.981926584678657 22.256873506122393 40-41 22.420355076834948 26.410204110927545 28.91529745354568 22.25414335869183 42-43 22.314926369138767 26.392740434063715 28.725933893297096 22.566399303500425 44-45 22.355471522260032 26.586723763018092 28.60956497413906 22.448239740582807 46-47 22.45860311466413 26.835603524593775 28.249894179399632 22.45589918134246 48-49 22.512349705469788 26.812864657643264 28.223992511728795 22.450793125158146 50-51 22.389231104090246 26.951583633671127 28.176714796382324 22.4824704658563 52-53 22.7547223100418 26.96022479734821 27.812395569352965 22.472657323257025 54-55 22.35308492529097 26.873851789114116 28.02954910177902 22.743514183815897 56-57 22.431925672630243 27.19277159117543 27.9304896997912 22.44481303640313 58-59 22.58423394787031 26.80494639050789 27.97974084738791 22.63107881423389 60-61 22.43369629588581 26.938035088234685 28.21430169044985 22.41396692542966 62-63 22.354887897856518 27.001874072854793 28.123847067909015 22.519390961379678 64-65 22.150411261228925 26.908787985529482 28.044693670066156 22.896107083175433 66-67 22.183090127494435 27.120244332059766 27.832000341872583 22.864665198573217 68-69 22.22041906670006 26.962517739377244 27.984072126220887 22.832991067701812 70-71 22.256525587353774 26.96380273446825 27.796543168987682 22.983128509190294 72-73 22.37442876643595 26.949672023718314 27.797179160277896 22.878720049567832 74-75 22.262728150503545 27.248351680638038 27.50693449324446 22.981985675613956 76-77 22.31348501209009 26.948855664781533 27.727249355094834 23.01040996803354 78-79 22.282895595756397 27.1107917541346 27.729275065970327 22.877037584138673 80-81 22.230794860512308 27.305414650464492 27.6191376007489 22.8446528882743 82-83 22.340296577639503 27.17077808302798 27.60642470235879 22.88250063697373 84-85 22.364149941736812 27.05722480242268 27.584965759942303 22.993659495898207 86-87 22.404154994879384 27.043763483982175 27.62838520788164 22.9236963132568 88-89 22.25801130945544 27.129203984219114 27.424327616279847 23.188457090045596 90-91 22.34668695024724 27.17394316746409 27.47178591117007 23.007583971118603 92-93 22.437928405936756 26.900745884181106 27.562956411234975 23.098369298647164 94-95 22.207105664847337 27.01015342990421 27.505438622028787 23.277302283219665 96-97 22.285189069142337 26.87202005056712 27.47264230724416 23.370148573046386 98-99 22.23350274143325 26.834763837452353 27.27999009124534 23.651743329869053 100-101 22.66585340774185 26.417894901307225 27.186918683455325 23.729333007495605 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1064.0 1 705.0 2 508.0 3 645.5 4 476.5 5 417.5 6 844.0 7 1755.0 8 2683.5 9 3074.5 10 2502.0 11 1763.5 12 1645.0 13 1710.0 14 1776.5 15 1855.5 16 1962.5 17 2059.5 18 2156.5 19 2252.0 20 2512.5 21 2952.0 22 3483.0 23 4204.5 24 5114.0 25 6293.0 26 7833.0 27 9753.5 28 11527.0 29 13364.5 30 15767.0 31 17989.0 32 20353.5 33 22728.5 34 24896.5 35 27267.0 36 30517.5 37 34055.0 38 37071.0 39 39204.0 40 40912.5 41 44059.5 42 47435.0 43 50477.5 44 53225.5 45 55750.0 46 57222.5 47 58708.5 48 62385.0 49 63202.0 50 60968.0 51 58699.0 52 55867.0 53 54330.5 54 52951.5 55 48756.0 56 43476.5 57 39256.0 58 35385.0 59 30987.0 60 25563.5 61 20436.5 62 16336.0 63 12677.5 64 9872.5 65 7501.0 66 5702.0 67 4701.5 68 3585.0 69 2465.0 70 1779.0 71 1296.5 72 1001.0 73 782.5 74 592.5 75 505.5 76 335.0 77 176.0 78 98.5 79 59.0 80 37.0 81 23.5 82 12.5 83 8.0 84 3.5 85 0.5 86 0.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008812813831310726 2 0.013553039452697556 3 0.009413687501627367 4 0.0 5 8.679286349018139E-4 6 0.001602329787511041 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.679286349018139E-4 14-15 0.0 16-17 0.002436876551839708 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003204659575022082 26-27 0.01832664694465753 28-29 0.006609610373483044 30-31 0.0033715689278878153 32-33 0.0 34-35 0.008979723184176459 36-37 7.010192820360805E-4 38-39 0.0026037859047054416 40-41 0.002036294104961948 42-43 0.002904222739863762 44-45 0.0 46-47 6.676374114629337E-5 48-49 6.676374114629338E-4 50-51 0.004072588209923896 52-53 0.007644448361250592 54-55 0.007243865914372831 56-57 0.01522213298135489 58-59 0.021564688390252763 60-61 0.0034717145396072555 62-63 0.018593701909242706 64-65 0.019461630544144518 66-67 0.012351292112064274 68-69 0.02964310106895426 70-71 0.015589333557659504 72-73 0.004907134974252564 74-75 0.015555951687086357 76-77 0.020830287237643533 78-79 0.02440214738897023 80-81 0.010682198583406941 82-83 0.03368230740830501 84-85 0.020296177308473187 86-87 0.02940942797494223 88-89 0.02076352349649724 90-91 0.025670658470749803 92-93 0.022332471413435136 94-95 0.013152457005819795 96-97 0.013719948805563289 98-99 0.010248234265956033 100-101 0.009413687501627367 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1497819.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.690337636404124 #Duplication Level Percentage of deduplicated Percentage of total 1 81.42479436265691 51.04547850569736 2 11.173602247893294 14.009537950706294 3 3.1564029345215947 5.936278970450867 4 1.3554223048157277 3.3988752771524418 5 0.752759910958763 2.359538648857718 6 0.455735430316286 1.7142124799639935 7 0.3070304127153148 1.3473488166437315 8 0.21462472650039688 1.0763917255544617 9 0.14896794542192365 0.8404965715951633 >10 0.9154363103044247 10.395407321836192 >50 0.06467380033090839 2.7690614752507487 >100 0.028846563945011297 3.378116854531103 >500 9.579654109474563E-4 0.3672040314830873 >1k 5.322030060819202E-4 0.5468357328736871 >5k 2.1288120243276808E-4 0.8152156374030306 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6418 0.42848969067691095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5799 0.38716293490735526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3473 0.23187047300107688 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1561 0.10421819992936397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.676374114629338E-5 0.0 0.0 0.0 0.0 7 6.676374114629338E-5 0.0 0.0 0.0 0.0 8 6.676374114629338E-5 0.0 0.0 0.0 0.0 9 6.676374114629338E-5 0.0 0.0 0.0 0.0 10-11 6.676374114629338E-5 0.0 0.0 0.0 0.0 12-13 6.676374114629338E-5 0.0 0.0 0.0 0.0 14-15 6.676374114629338E-5 0.0 0.0 0.0 0.0 16-17 6.676374114629338E-5 0.0 0.0 0.0 0.0 18-19 6.676374114629338E-5 0.0 0.0 0.0 0.0 20-21 6.676374114629338E-5 0.0 0.0 3.338187057314669E-5 0.0 22-23 6.676374114629338E-5 0.0 0.0 3.0043683515832017E-4 0.0 24-25 6.676374114629338E-5 0.0 0.0 5.674917997434937E-4 0.0 26-27 6.676374114629338E-5 0.0 0.0 0.0010348379877675473 0.0 28-29 6.676374114629338E-5 0.0 0.0 0.0032380414455952288 0.0 30-31 6.676374114629338E-5 0.0 0.0 0.010114706783663448 0.0 32-33 6.676374114629338E-5 0.0 0.0 0.025169930412152604 0.0 34-35 6.676374114629338E-5 0.0 0.0 0.04867076729564787 0.0 36-37 6.676374114629338E-5 0.0 0.0 0.08672609974903508 0.0 38-39 6.676374114629338E-5 0.0 0.0 0.13990341957205776 0.0 40-41 6.676374114629338E-5 0.0 0.0 0.19678612702869974 0.0 42-43 6.676374114629338E-5 0.0 0.0 0.2565396753546323 0.0 44-45 6.676374114629338E-5 0.0 0.0 0.3307475736387374 0.0 46-47 6.676374114629338E-5 0.0 0.0 0.41690618158802895 0.0 48-49 6.676374114629338E-5 0.0 0.0 0.5046671193248315 0.0 50-51 6.676374114629338E-5 0.0 0.0 0.5974019557770331 0.0 52-53 6.676374114629338E-5 0.0 0.0 0.6896026823000643 0.0 54-55 6.676374114629338E-5 0.0 0.0 0.7896815302783581 0.0 56-57 6.676374114629338E-5 0.0 0.0 0.906417931672652 6.676374114629338E-5 58-59 6.676374114629338E-5 0.0 0.0 1.0340702047443648 6.676374114629338E-5 60-61 6.676374114629338E-5 0.0 0.0 1.171036019705986 6.676374114629338E-5 62-63 1.3352748229258677E-4 0.0 0.0 1.2979872734956626 6.676374114629338E-5 64-65 1.3352748229258677E-4 0.0 0.0 1.4274087857077524 6.676374114629338E-5 66-67 1.3352748229258677E-4 0.0 0.0 1.5826344838728845 6.676374114629338E-5 68-69 1.3352748229258677E-4 0.0 0.0 1.7535162793368224 6.676374114629338E-5 70-71 1.3352748229258677E-4 0.0 0.0 1.9336448529495218 6.676374114629338E-5 72-73 1.3352748229258677E-4 0.0 0.0 2.1128721160567467 6.676374114629338E-5 74-75 1.3352748229258677E-4 0.0 0.0 2.29166541484652 6.676374114629338E-5 76-77 1.3352748229258677E-4 0.0 0.0 2.488017577557769 6.676374114629338E-5 78-79 1.3352748229258677E-4 0.0 0.0 2.7025294778608098 6.676374114629338E-5 80-81 1.3352748229258677E-4 0.0 0.0 2.936436244966848 1.6690935286573346E-4 82-83 1.3352748229258677E-4 0.0 0.0 3.17227916056613 2.0029122343888012E-4 84-85 1.3352748229258677E-4 0.0 0.0 3.404750507237523 2.3367309401202684E-4 86-87 1.6690935286573346E-4 0.0 0.0 3.6508082752321878 2.6705496458517353E-4 88-89 2.0029122343888012E-4 0.0 0.0 3.9116875937613287 3.338187057314669E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4080 0.0 42.26803 1 GTATCAA 6160 0.0 32.160355 1 GTATTCG 100 4.9485105E-5 28.504719 1 ATCAACG 7320 0.0 26.600714 3 TATCAAC 7430 0.0 26.593071 2 TCAACGC 7410 0.0 26.275875 4 CAACGCA 7555 0.0 25.77157 5 AACGCAG 7740 0.0 25.155582 6 GTACATG 5655 0.0 23.186861 1 GAGTACT 5020 0.0 23.131067 12-13 TACATGG 5570 0.0 22.597202 2 ACGCAGA 8755 0.0 22.130718 7 CGCAGAG 8885 0.0 21.806915 8 ACATGGG 5620 0.0 21.464357 3 GTACTTT 5390 0.0 21.411053 14-15 GTATTAG 410 0.0 20.857111 1 CATGGGG 3480 0.0 20.469324 4 GCAGAGT 9480 0.0 20.28795 9 GTATAGC 555 0.0 19.687943 1 GTACCTG 1125 0.0 19.425438 1 >>END_MODULE