FastQCFastQC Report
Thu 26 May 2016
SRR938195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476803
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262111.7748474237931533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206271.3967333489978013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141950.9611979390616081No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93190.63102526200177No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46500.31486934953409496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41090.27823616284636477No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40410.27363162182091993No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35600.2410612654497587No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26350.17842596473598712No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22920.15520011809293455No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22140.14991843868139487No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19060.12906257638967417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111650.040.3265651
GTATCAA180400.035.427971
TCGCGAT653.046771E-729.22291450-51
TATCAAC222050.028.7464732
ATCAACG224500.028.3480763
TCAACGC227500.027.9742574
CAACGCA229400.027.7425615
AACGCAG237050.026.820856
ATCGCGT751.0597832E-625.3368224-25
ACGCAGA260000.024.4284957
GAGTACT142000.024.38685412-13
CGCAGAG260400.024.2815138
CATTCCG2002.0550942E-823.7500519
GCAGAGT263750.023.7365449
AGTACTT144750.023.11953212-13
CCGACCA1852.5426016E-723.1081589
GTACATG142450.022.9019071
GTACTTT150100.022.84931214-15
TACATGG142900.022.4840132
TTCGCGA853.1563395E-622.34920348-49
Produced by FastQC (version 0.11.5)