##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938195_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1476803 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.958205664533455 33.0 31.0 34.0 30.0 34.0 2 32.08565191159552 33.0 31.0 34.0 30.0 34.0 3 32.127230239916905 34.0 31.0 34.0 30.0 34.0 4 35.68873031812638 37.0 35.0 37.0 35.0 37.0 5 35.730876088415314 37.0 35.0 37.0 35.0 37.0 6 35.803989428515514 37.0 36.0 37.0 35.0 37.0 7 35.75235627229901 37.0 36.0 37.0 35.0 37.0 8 35.73619230188454 37.0 36.0 37.0 35.0 37.0 9 37.3470381628423 39.0 37.0 39.0 35.0 39.0 10-11 37.26062176200888 39.0 37.0 39.0 34.0 39.0 12-13 37.12133744311191 39.0 37.0 39.0 33.5 39.0 14-15 38.180543715038496 40.0 38.0 41.0 33.0 41.0 16-17 38.02571297593518 40.0 38.0 41.0 33.0 41.0 18-19 38.31934895852731 40.0 38.0 41.0 34.0 41.0 20-21 38.36723584662274 40.0 38.0 41.0 34.0 41.0 22-23 38.242454477679146 40.0 38.0 41.0 34.0 41.0 24-25 38.102127027098405 40.0 38.0 41.0 33.5 41.0 26-27 37.873291495209585 40.0 37.5 41.0 33.0 41.0 28-29 37.64675213958802 40.0 37.0 41.0 33.0 41.0 30-31 37.29029836748707 39.5 36.5 41.0 32.5 41.0 32-33 36.84758190496633 39.0 35.0 41.0 31.5 41.0 34-35 36.5203794954371 39.0 35.0 41.0 31.0 41.0 36-37 36.186344082453786 38.0 35.0 40.0 30.0 41.0 38-39 35.841470053893445 38.0 35.0 40.0 30.0 41.0 40-41 35.579480472344656 38.0 35.0 40.0 29.0 41.0 42-43 35.22430412180907 38.0 35.0 40.0 26.5 41.0 44-45 34.69837446159034 38.0 34.5 40.0 24.5 41.0 46-47 34.01493970421241 38.0 33.5 40.0 21.5 41.0 48-49 33.37121031037992 38.0 33.0 40.0 16.0 41.0 50-51 32.48972340928343 37.0 32.0 39.5 9.0 40.5 52-53 32.46674167102857 37.0 32.5 39.5 8.0 40.5 54-55 32.46235990853215 37.0 32.5 40.0 4.5 41.0 56-57 32.01950023124276 36.5 31.5 40.0 2.0 41.0 58-59 31.591500694405415 36.0 31.0 40.0 2.0 41.0 60-61 31.05982856210341 35.5 30.5 39.0 2.0 41.0 62-63 30.47784978768326 35.0 29.0 39.0 2.0 40.5 64-65 29.900883530166176 35.0 28.0 38.5 2.0 40.0 66-67 29.244568503720537 35.0 27.5 38.0 2.0 40.0 68-69 28.57746259995409 34.5 26.0 37.5 2.0 40.0 70-71 27.80104489224358 34.0 25.0 37.0 2.0 39.0 72-73 27.02765602453408 34.0 22.5 36.0 2.0 39.0 74-75 26.237288588931634 33.0 19.0 35.5 2.0 38.5 76-77 25.426449567071572 32.5 14.0 35.0 2.0 37.5 78-79 24.615110139944193 32.0 6.5 35.0 2.0 37.0 80-81 23.830592164290024 31.0 2.0 35.0 2.0 36.5 82-83 23.030750885527723 31.0 2.0 35.0 2.0 36.0 84-85 22.231157439414737 30.0 2.0 34.5 2.0 35.5 86-87 21.463356317667284 29.0 2.0 34.0 2.0 35.0 88-89 20.66263577471064 29.0 2.0 34.0 2.0 35.0 90-91 19.825269856575318 27.0 2.0 34.0 2.0 35.0 92-93 18.947345041958883 25.5 2.0 34.0 2.0 35.0 94-95 18.05038620587851 24.0 2.0 33.5 2.0 35.0 96-97 16.992139777614213 16.5 2.0 33.0 2.0 35.0 98-99 15.755215150565105 2.0 2.0 32.5 2.0 35.0 100-101 12.813226950378622 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2060492208559488 1101 2 0.2544332138829404 1101 3 0.14504682037897254 1101 4 0.1352946639740722 1101 5 0.24691976619192246 1101 6 0.15833903291730422 1101 7 0.19377999603150187 1101 8 0.08037715999819284 1101 9 0.05848272715780212 1101 10-11 0.24294778112126636 1101 12-13 0.2809491083460074 1101 14-15 0.5188907251786006 1101 16-17 0.5424505359980287 1101 18-19 0.4952647326504973 1101 20-21 0.4703036896900201 1101 22-23 0.6480405058188978 1101 24-25 0.6477188323161087 1101 26-27 0.5427447398598773 1101 28-29 0.7255145902009588 1101 30-31 0.7859476331218076 1101 32-33 0.7487562803400891 1101 34-35 0.8718329692025364 1101 36-37 0.8411434991604025 1101 38-39 0.93690371727849 1101 40-41 0.9468652852490536 1101 42-43 1.0908962950383057 1101 44-45 1.3281885432968679 1101 46-47 1.4357891183222975 1101 48-49 1.5744182060807717 1101 50-51 1.7074646702842244 1101 52-53 1.817471654753831 1101 54-55 1.9445911356633303 1101 56-57 2.2657480133340115 1101 58-59 2.410309390877842 1101 60-61 2.5770415097726556 1101 62-63 2.7445221041748944 1101 64-65 2.792057399550057 1101 66-67 3.005632424721508 1101 68-69 3.194996326768756 1101 70-71 3.3509207856724217 1101 72-73 3.7542536476688895 1101 74-75 3.886851048733881 1101 76-77 3.7938152558984477 1101 78-79 3.97822450037231 1101 80-81 4.162435293732678 1101 82-83 4.3637683534177185 1101 84-85 4.499626026530883 1101 86-87 4.750728719488315 1101 88-89 4.794622658545013 1101 90-91 4.818217086171167 1101 92-93 5.159209688953645 1101 94-95 5.1723424397772 1101 96-97 5.300215976881265 1101 98-99 5.004874616477393 1101 100-101 4.1279371057866 1102 1 0.6367771721998245 1102 2 0.566644237130717 1102 3 0.5419334334634129 1102 4 0.3646125708101309 1102 5 0.451381621260623 1102 6 0.40221515641570704 1102 7 0.4672384265906544 1102 8 0.5798343158187791 1102 9 0.6300247287134084 1102 10-11 0.5631022891586 1102 12-13 0.7199000687210884 1102 14-15 0.7997119863055602 1102 16-17 0.7881773260334626 1102 18-19 0.7578487249588548 1102 20-21 0.7977055726301288 1102 22-23 0.7840297245553955 1102 24-25 0.7921808764350331 1102 26-27 0.7653095030609833 1102 28-29 0.8594782709545683 1102 30-31 0.9409384236757816 1102 32-33 0.9086056613356845 1102 34-35 1.1642126252391805 1102 36-37 1.0893495476868864 1102 38-39 1.2253301983518625 1102 40-41 1.240605427630868 1102 42-43 1.45888320736222 1102 44-45 1.5143712684286612 1102 46-47 1.556414631458587 1102 48-49 1.7328416083364075 1102 50-51 2.0826132097363015 1102 52-53 1.951074114567163 1102 54-55 2.062814360751915 1102 56-57 2.583694009790669 1102 58-59 2.740193233026318 1102 60-61 2.801595351272951 1102 62-63 2.874101845554204 1102 64-65 2.8871766632264944 1102 66-67 3.2554345971785104 1102 68-69 3.2895882753903116 1102 70-71 3.723606270937303 1102 72-73 3.932151922450675 1102 74-75 3.938143071895233 1102 76-77 3.933194550038966 1102 78-79 4.235236912869066 1102 80-81 4.664679589817716 1102 82-83 4.946232055777063 1102 84-85 5.163248309609269 1102 86-87 5.298627966528326 1102 88-89 5.253839801618209 1102 90-91 5.353077270036003 1102 92-93 5.472875048473131 1102 94-95 5.356924989902513 1102 96-97 5.1768431590608515 1102 98-99 5.415584175936386 1102 100-101 4.547032392494749 1103 1 0.4922276081597765 1103 2 0.41336966052669766 1103 3 0.42727900390624285 1103 4 0.3795930821280322 1103 5 0.36253726070515313 1103 6 0.332320745410577 1103 7 0.43767467075923605 1103 8 0.32452935056509347 1103 9 0.3474672606788545 1103 10-11 0.3653515944261656 1103 12-13 0.41620671476863436 1103 14-15 0.5447429214393154 1103 16-17 0.6249594519986914 1103 18-19 0.6199703364022042 1103 20-21 0.5844469383752653 1103 22-23 0.5793614197283716 1103 24-25 0.6142854473933355 1103 26-27 0.682639895973999 1103 28-29 0.6681687891325936 1103 30-31 0.6627505675083256 1103 32-33 0.7956917771515606 1103 34-35 0.5588955804907911 1103 36-37 0.6797836214221888 1103 38-39 0.9383553172412178 1103 40-41 1.114706409622734 1103 42-43 1.0930376233541779 1103 44-45 1.172555481368832 1103 46-47 1.4879933374411038 1103 48-49 1.5696124299616727 1103 50-51 1.6432241737833309 1103 52-53 1.8011572499130608 1103 54-55 1.8437940659088952 1103 56-57 2.0541832228664916 1103 58-59 2.171882398559319 1103 60-61 2.056530826100449 1103 62-63 2.2040212682189555 1103 64-65 2.2159517637059274 1103 66-67 2.652464656387412 1103 68-69 2.7955921718348016 1103 70-71 3.176924191610553 1103 72-73 3.2447750590109727 1103 74-75 3.4391927606060086 1103 76-77 3.733043782441989 1103 78-79 3.9364312325351314 1103 80-81 3.9422721917408765 1103 82-83 4.26728661504346 1103 84-85 4.524038592786212 1103 86-87 4.519324533497173 1103 88-89 4.864432420336389 1103 90-91 4.9957480527743385 1103 92-93 5.01835305410334 1103 94-95 5.077017905117376 1103 96-97 5.021205329442445 1103 98-99 4.973967216067434 1103 100-101 4.177383705542283 1104 1 0.5113989241289367 1104 2 0.4103085585658377 1104 3 0.3288727005691996 1104 4 0.21321048142202415 1104 5 0.37276960933622405 1104 6 0.2502725790548581 1104 7 0.21705608203237858 1104 8 0.3424198307411075 1104 9 0.32384109745712664 1104 10-11 0.4585010346280427 1104 12-13 0.4800262698574187 1104 14-15 0.7134882579484554 1104 16-17 0.643178803491935 1104 18-19 0.534390611841637 1104 20-21 0.6072910987736648 1104 22-23 0.5605772220399743 1104 24-25 0.6370626984592533 1104 26-27 0.8470241777769942 1104 28-29 0.7611805275386132 1104 30-31 0.830236574795471 1104 32-33 0.9132783575290802 1104 34-35 1.0206410657537148 1104 36-37 1.2323020707745158 1104 38-39 1.2780764009095478 1104 40-41 1.4488868628976164 1104 42-43 1.3786768494282668 1104 44-45 1.2688971538751375 1104 46-47 1.5664304907596573 1104 48-49 1.79231118204288 1104 50-51 1.896349646106465 1104 52-53 1.8383552543422184 1104 54-55 1.7348662700397028 1104 56-57 2.1363177142092056 1104 58-59 2.1653019851910784 1104 60-61 2.258576997124287 1104 62-63 2.350877694531764 1104 64-65 2.5095523042377472 1104 66-67 2.6119967570357794 1104 68-69 2.8834975931037263 1104 70-71 3.2824025724286052 1104 72-73 3.530331843228975 1104 74-75 3.5359938373683377 1104 76-77 3.860125812686203 1104 78-79 3.826232458051244 1104 80-81 4.1579132040551805 1104 82-83 4.481847220859674 1104 84-85 4.507783209422623 1104 86-87 4.6149920347382825 1104 88-89 4.717733708878704 1104 90-91 4.906838991518249 1104 92-93 5.2024842513562355 1104 94-95 5.082545990010981 1104 96-97 5.2931630905367015 1104 98-99 4.939448870663538 1104 100-101 4.143792831019386 1105 1 0.4421358169192793 1105 2 0.31444969772002906 1105 3 0.3271907889478456 1105 4 0.1531667416182927 1105 5 0.19471426282707682 1105 6 0.22546143634827587 1105 7 0.39921771727474464 1105 8 0.36877988104693316 1105 9 0.3407915340975407 1105 10-11 0.35895004947229836 1105 12-13 0.3273241837145875 1105 14-15 0.47455242481294846 1105 16-17 0.5841898360913831 1105 18-19 0.5946144963335414 1105 20-21 0.5707259360626011 1105 22-23 0.5735678427310233 1105 24-25 0.6005286822597071 1105 26-27 0.5499479092300916 1105 28-29 0.6545550752828433 1105 30-31 0.7732696052500216 1105 32-33 0.8741911691836037 1105 34-35 0.7680723203520401 1105 36-37 0.8225368817993726 1105 38-39 0.8014910013839867 1105 40-41 1.0371249299956204 1105 42-43 1.0744528773894118 1105 44-45 1.1916647487078436 1105 46-47 1.2562979413082545 1105 48-49 1.3358666320931363 1105 50-51 1.437707162601491 1105 52-53 1.4420866064207019 1105 54-55 1.6683297452832804 1105 56-57 1.9188805501916733 1105 58-59 2.0072780545686726 1105 60-61 2.2194662384457757 1105 62-63 2.214571300405492 1105 64-65 2.223938793722848 1105 66-67 2.3169736328554755 1105 68-69 2.4160532738065257 1105 70-71 2.7470908463562793 1105 72-73 2.8576067591754857 1105 74-75 3.0723258221419023 1105 76-77 3.5518216476399935 1105 78-79 3.489360207572684 1105 80-81 3.558775541525108 1105 82-83 3.8079251006072 1105 84-85 4.038796716907047 1105 86-87 4.387371645053335 1105 88-89 4.259969220279167 1105 90-91 4.434987019944074 1105 92-93 4.612492719840173 1105 94-95 4.569626922116637 1105 96-97 4.317869850339857 1105 98-99 4.41228827083224 1105 100-101 3.8312830451221913 1106 1 0.267562616773386 1106 2 0.2983269795924386 1106 3 0.26650504263504615 1106 4 0.2305656290132987 1106 5 0.2779376940615208 1106 6 0.22014281652610634 1106 7 0.14096275937981062 1106 8 0.18104409959755685 1106 9 0.3243778829198547 1106 10-11 0.18954679380311745 1106 12-13 0.23232999702754853 1106 14-15 0.47825727537283313 1106 16-17 0.47552152864788866 1106 18-19 0.35030533932275176 1106 20-21 0.47852796895728744 1106 22-23 0.40686400722799476 1106 24-25 0.543338143573628 1106 26-27 0.4566102760774129 1106 28-29 0.5145336510585992 1106 30-31 0.514702322168958 1106 32-33 0.5022391474663053 1106 34-35 0.6083228008728909 1106 36-37 0.7692493383332177 1106 38-39 1.0068651337991597 1106 40-41 1.0354079436769652 1106 42-43 0.902595943716797 1106 44-45 1.1309790023950441 1106 46-47 1.3156846501602146 1106 48-49 1.4182354474929113 1106 50-51 1.4363115931267814 1106 52-53 1.4096620110262137 1106 54-55 1.720393391166418 1106 56-57 1.9357426340210324 1106 58-59 2.0454592737706747 1106 60-61 2.136770925569742 1106 62-63 1.8383964710345175 1106 64-65 2.1838713229257927 1106 66-67 2.4922466232514786 1106 68-69 2.3217535293355915 1106 70-71 2.3720976650996413 1106 72-73 2.7697733226250953 1106 74-75 2.9677597241851714 1106 76-77 2.8653857058565535 1106 78-79 3.2701295864413176 1106 80-81 3.394624055252976 1106 82-83 3.341037185773576 1106 84-85 3.5272896195311105 1106 86-87 3.4539316719218576 1106 88-89 3.746213007200655 1106 90-91 3.855232590969706 1106 92-93 3.945161827349562 1106 94-95 3.8703802996675165 1106 96-97 3.9266619647706733 1106 98-99 3.6874160086245507 1106 100-101 3.1076903000512033 1107 1 0.45673889288634584 1107 2 0.32548281905297927 1107 3 0.34227090500667856 1107 4 0.29273078346601267 1107 5 0.31797577338080174 1107 6 0.3430879108179994 1107 7 0.3707993091763555 1107 8 0.34178788559137274 1107 9 0.4378943001454729 1107 10-11 0.4226341171178376 1107 12-13 0.4341788104542559 1107 14-15 0.5940635214873211 1107 16-17 0.5306530702662897 1107 18-19 0.4802182423536294 1107 20-21 0.5634644129551916 1107 22-23 0.5031604912921352 1107 24-25 0.5102770906876231 1107 26-27 0.6519183672670366 1107 28-29 0.6048667990138625 1107 30-31 0.6526922298179016 1107 32-33 0.91656221536077 1107 34-35 1.036846751003118 1107 36-37 1.1488393660170289 1107 38-39 1.2779442083016903 1107 40-41 1.2210238853719702 1107 42-43 1.1078770680297012 1107 44-45 1.3129019772932153 1107 46-47 1.4611285999217287 1107 48-49 1.5486084670968907 1107 50-51 1.6352394216530897 1107 52-53 1.6946393201354368 1107 54-55 1.8668098076751036 1107 56-57 2.1308825022899427 1107 58-59 2.252952132618077 1107 60-61 2.2358511533082392 1107 62-63 2.1594692467792846 1107 64-65 2.350439155425942 1107 66-67 2.428502938652297 1107 68-69 2.5315001144359037 1107 70-71 2.6459991463831116 1107 72-73 2.892781404494606 1107 74-75 3.097153875865402 1107 76-77 3.2538308651844012 1107 78-79 3.29478512632679 1107 80-81 3.316543527427317 1107 82-83 3.5892940173652725 1107 84-85 3.5903947227539277 1107 86-87 3.7352413628935324 1107 88-89 3.863913745537971 1107 90-91 3.751536858864579 1107 92-93 3.9239826384452066 1107 94-95 3.5935542488247627 1107 96-97 3.4688946234712006 1107 98-99 3.377527112566339 1107 100-101 2.622536420886984 1108 1 0.548741216784169 1108 2 0.3880810422647869 1108 3 0.43280507705217985 1108 4 0.28989992713196955 1108 5 0.3064531133492139 1108 6 0.27631944688201315 1108 7 0.3517407108558146 1108 8 0.2748566879728571 1108 9 0.3224040814030076 1108 10-11 0.44770220164607366 1108 12-13 0.49749944954643155 1108 14-15 0.5444112443310658 1108 16-17 0.6051802807574944 1108 18-19 0.5647183407035854 1108 20-21 0.48484123116189437 1108 22-23 0.5138271518752973 1108 24-25 0.654831494433445 1108 26-27 0.6398431224567744 1108 28-29 0.6252987479448961 1108 30-31 0.555353407137801 1108 32-33 0.7044791633894079 1108 34-35 0.7537512319080193 1108 36-37 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0.19830617173192167 2311 40-41 0.3844918034676752 2311 42-43 0.3235316695322368 2311 44-45 0.2964008408677188 2311 46-47 0.3955246952453777 2311 48-49 0.8057725836111302 2311 50-51 0.7519516389077054 2311 52-53 0.7033047772234724 2311 54-55 0.9773909838414667 2311 56-57 1.2347036054392717 2311 58-59 1.2233312785296704 2311 60-61 1.266629060379941 2311 62-63 1.3004115272518142 2311 64-65 1.5288836900358866 2311 66-67 1.6537852587296022 2311 68-69 1.8669978449873774 2311 70-71 2.2247346773728225 2311 72-73 2.4675001051587664 2311 74-75 2.638416711689345 2311 76-77 2.542692406708163 2311 78-79 2.6832789032063182 2311 80-81 2.8786707441375334 2311 82-83 3.1313385710290547 2311 84-85 3.589106036652339 2311 86-87 3.550576897625966 2311 88-89 3.6360478862778045 2311 90-91 4.043862329086149 2311 92-93 4.122819886322066 2311 94-95 4.1969976776314475 2311 96-97 4.2722396357881465 2311 98-99 3.9854698846960144 2311 100-101 3.386113345665141 2312 1 -0.26196867646534017 2312 2 -0.2222829754204696 2312 3 -0.12212077364942076 2312 4 -0.2716252028507 2312 5 -0.26613978639404934 2312 6 -0.24166790329237386 2312 7 -0.2165984739736686 2312 8 -0.2302842693550886 2312 9 -0.1534897408406195 2312 10-11 -0.12524451911589551 2312 12-13 -0.14676062173091964 2312 14-15 -0.1618566846696723 2312 16-17 -0.046647215149775434 2312 18-19 -0.09411688050283118 2312 20-21 -0.16202470088129672 2312 22-23 -0.07684186366633838 2312 24-25 -0.052106004635454894 2312 26-27 5.236931045260462E-5 2312 28-29 0.03296635978957596 2312 30-31 -0.03465514250225965 2312 32-33 -0.1820001560824167 2312 34-35 0.05293835398165214 2312 36-37 0.11257852492889242 2312 38-39 0.07649897519146265 2312 40-41 0.32074717775110884 2312 42-43 0.3598594647150932 2312 44-45 0.6597419787160064 2312 46-47 0.5295977910548189 2312 48-49 0.5149865286962338 2312 50-51 0.73603900364796 2312 52-53 0.5980363477362971 2312 54-55 0.8665675658527618 2312 56-57 0.8867720449251166 2312 58-59 1.2104935805005113 2312 60-61 1.3886787593089736 2312 62-63 1.2852454938340507 2312 64-65 1.4041199432576796 2312 66-67 1.4771542956357955 2312 68-69 1.8803491898080686 2312 70-71 2.254134056066775 2312 72-73 2.431824653552315 2312 74-75 2.1317809645111936 2312 76-77 2.4024085070614483 2312 78-79 2.872411671614973 2312 80-81 3.320661638407188 2312 82-83 3.3936662676139555 2312 84-85 3.5072370914005973 2312 86-87 3.679024701807627 2312 88-89 3.6281400864844464 2312 90-91 3.733799851305406 2312 92-93 3.815584635744365 2312 94-95 4.048886696595588 2312 96-97 3.996470328640214 2312 98-99 3.888117066460671 2312 100-101 3.396105936930752 2313 1 -0.2964778976101101 2313 2 -0.27724062936257 2313 3 -0.2952475443812723 2313 4 -0.13826426899117905 2313 5 -0.21015485272424428 2313 6 -0.2241804338653921 2313 7 -0.11667205341937859 2313 8 -0.1321989817529925 2313 9 -0.08965611338712165 2313 10-11 -0.11362957214645064 2313 12-13 -0.05441545310750939 2313 14-15 -0.059878010193173736 2313 16-17 -0.018600238922459766 2313 18-19 -0.2247783643308381 2313 20-21 -0.15648779818731384 2313 22-23 -0.15197112173916594 2313 24-25 -0.18646984544299272 2313 26-27 -0.08578197351706507 2313 28-29 -0.015290261866496735 2313 30-31 0.053617301698899666 2313 32-33 0.1645891698257813 2313 34-35 0.140279527445351 2313 36-37 0.016015877333593664 2313 38-39 0.12711415732529474 2313 40-41 0.30525053625427034 2313 42-43 0.33631700634479245 2313 44-45 0.5247566320263815 2313 46-47 0.6538531861672254 2313 48-49 0.752103703140115 2313 50-51 0.7809639651290894 2313 52-53 0.6956228167594745 2313 54-55 1.10787158861298 2313 56-57 1.27883933092388 2313 58-59 1.2383035808496388 2313 60-61 1.3257699800361067 2313 62-63 1.5151980591349847 2313 64-65 1.7855384653845405 2313 66-67 1.8352694761228143 2313 68-69 2.0597598559951855 2313 70-71 2.405891004120477 2313 72-73 2.8129460912564817 2313 74-75 2.94552895787389 2313 76-77 2.9551668469984875 2313 78-79 3.164405147614705 2313 80-81 3.3678661135130277 2313 82-83 3.8544025429394146 2313 84-85 3.9195416892266763 2313 86-87 3.985794340174863 2313 88-89 4.152845686361129 2313 90-91 3.9537133015885573 2313 92-93 4.192816144873948 2313 94-95 4.053468943858096 2313 96-97 4.196386571912171 2313 98-99 4.01610693133666 2313 100-101 3.7614707019277525 2314 1 -0.3084154323536126 2314 2 -0.21831596876585735 2314 3 -0.248066265997533 2314 4 -0.127737344082675 2314 5 -0.0941022329615393 2314 6 -0.1344683677599292 2314 7 -0.15605908795133416 2314 8 -0.08077512274694243 2314 9 -0.1206357741703954 2314 10-11 -0.13237446141310727 2314 12-13 -0.08690659450304139 2314 14-15 -0.11750589631481745 2314 16-17 -0.03858103236755994 2314 18-19 0.04077960917208401 2314 20-21 -0.01563235075426661 2314 22-23 0.03605153276229345 2314 24-25 0.061182458363340686 2314 26-27 0.0012135382076579049 2314 28-29 -0.008684807002531159 2314 30-31 0.09158977377919086 2314 32-33 0.09841588458156281 2314 34-35 0.052230406222712134 2314 36-37 0.21368528806485898 2314 38-39 0.2722136083226516 2314 40-41 0.2905188177387643 2314 42-43 0.5504444804456696 2314 44-45 0.6901736394086129 2314 46-47 0.4743749821603487 2314 48-49 0.5173872069419048 2314 50-51 0.49031288378802884 2314 52-53 0.8176702911639069 2314 54-55 0.9586195185485806 2314 56-57 1.1006280196100988 2314 58-59 1.4008060868086005 2314 60-61 1.533325991776323 2314 62-63 1.6035374605254198 2314 64-65 1.6202713412262106 2314 66-67 1.8198476307377938 2314 68-69 2.040261756097067 2314 70-71 2.2388716059427303 2314 72-73 2.5486845760193013 2314 74-75 2.8106038583786237 2314 76-77 3.044773773500687 2314 78-79 3.2987005162230822 2314 80-81 3.5566658524505996 2314 82-83 3.692732141840523 2314 84-85 3.9229120064036103 2314 86-87 4.124087859591064 2314 88-89 4.5228932948383225 2314 90-91 4.3259392761615345 2314 92-93 4.537902821014246 2314 94-95 4.442377806636525 2314 96-97 4.780285287533104 2314 98-99 4.396269760170652 2314 100-101 3.6233283305727753 2315 1 -0.3384662485608345 2315 2 -0.36256714173583404 2315 3 -0.2601950003891602 2315 4 -0.1760460188139774 2315 5 -0.11526995642543625 2315 6 -0.19245310790773118 2315 7 -0.09652227992867068 2315 8 -0.1875122425622635 2315 9 -0.2902761633116171 2315 10-11 -0.27782168900675686 2315 12-13 -0.24115926647618124 2315 14-15 -0.42474503228938687 2315 16-17 -0.20912352161221293 2315 18-19 -0.26946232993841335 2315 20-21 -0.3541117619458234 2315 22-23 -0.16020095933046008 2315 24-25 -0.14516656953311013 2315 26-27 -0.20044960580157323 2315 28-29 -0.06627950652735137 2315 30-31 -0.11601227086111976 2315 32-33 -0.18837609874952221 2315 34-35 0.04942187389671204 2315 36-37 -0.07191348874856374 2315 38-39 -0.06623417390216702 2315 40-41 0.12299037415374414 2315 42-43 0.012493467869028052 2315 44-45 0.01292760801948134 2315 46-47 0.20888878048445747 2315 48-49 0.4275134230082216 2315 50-51 0.4861684398432544 2315 52-53 0.7224507621507072 2315 54-55 0.6701027821782688 2315 56-57 0.991616612196033 2315 58-59 1.1430827728124129 2315 60-61 1.271412064537941 2315 62-63 1.221017036607325 2315 64-65 1.5713106001190482 2315 66-67 1.7073176131994607 2315 68-69 2.0139254697275035 2315 70-71 2.2071921227990217 2315 72-73 2.4586273140984787 2315 74-75 2.596936397240281 2315 76-77 2.7395891712611764 2315 78-79 3.1566215487364637 2315 80-81 3.3386710689816113 2315 82-83 3.6009198470165806 2315 84-85 3.836136961808265 2315 86-87 3.934613410099974 2315 88-89 3.977051979313149 2315 90-91 4.139690681476999 2315 92-93 4.3065487714572015 2315 94-95 4.482034385011904 2315 96-97 4.44860235668212 2315 98-99 4.389428759436141 2315 100-101 4.197236981320028 2316 1 -0.6249665287453254 2316 2 -0.5790164139745784 2316 3 -0.509325853730541 2316 4 -0.4065381377271464 2316 5 -0.3471874241323789 2316 6 -0.3910113790975842 2316 7 -0.4877391427244788 2316 8 -0.45728565233567053 2316 9 -0.5117259384456005 2316 10-11 -0.43367452749117064 2316 12-13 -0.42941522578477276 2316 14-15 -0.3978299353742898 2316 16-17 -0.21410764449107944 2316 18-19 -0.2775539143457806 2316 20-21 -0.4312903759665474 2316 22-23 -0.5381138004011987 2316 24-25 -0.49810204088962706 2316 26-27 -0.3398227109641496 2316 28-29 -0.3612577383476818 2316 30-31 -0.45039191366181797 2316 32-33 -0.2963784551729276 2316 34-35 -0.39856841304094814 2316 36-37 -0.2255887908449168 2316 38-39 -0.2994607497603212 2316 40-41 -0.32434600528790014 2316 42-43 -0.3146434776889677 2316 44-45 -0.08504328153034635 2316 46-47 0.1904533594174609 2316 48-49 0.33178214148746576 2316 50-51 0.1836907468392468 2316 52-53 0.2551336364125305 2316 54-55 0.6387074634145264 2316 56-57 0.6569544859549552 2316 58-59 0.7510897271351524 2316 60-61 0.8747222646902486 2316 62-63 1.1563251677049315 2316 64-65 1.6597474580295888 2316 66-67 1.7619497094105014 2316 68-69 1.79192365930464 2316 70-71 2.0387364422380756 2316 72-73 2.6683327611723477 2316 74-75 2.7515506765914033 2316 76-77 2.9566400498910035 2316 78-79 3.1491026901649732 2316 80-81 3.187637978474836 2316 82-83 3.485146609664394 2316 84-85 3.3885522958025476 2316 86-87 3.6691107303867696 2316 88-89 3.9877670963440544 2316 90-91 4.0929919731993465 2316 92-93 4.336251488528337 2316 94-95 4.3363052587564646 2316 96-97 4.390119950831291 2316 98-99 4.2569803780403905 2316 100-101 4.02403210179936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4138.0 3 1100.0 4 1067.0 5 1278.0 6 1460.0 7 1836.0 8 2543.0 9 3145.0 10 4144.0 11 5730.0 12 8153.0 13 10948.0 14 13180.0 15 17593.0 16 23097.0 17 31808.0 18 30078.0 19 21780.0 20 21789.0 21 23046.0 22 25478.0 23 28330.0 24 31849.0 25 35259.0 26 38763.0 27 42248.0 28 46334.0 29 51054.0 30 56172.0 31 63103.0 32 71517.0 33 83835.0 34 105633.0 35 148747.0 36 149337.0 37 164090.0 38 99882.0 39 7259.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.76065986983695 19.491964396177497 15.50740341436688 19.239972319618673 2 15.294575822329989 21.676797984347537 41.30990317959694 21.718723013725537 3 18.906333092840423 28.661538409427322 30.61583120785023 21.816297289882023 4 11.71709727262197 18.503332423371873 38.12063379380093 31.658936510205226 5 11.930869631203521 39.526171866530795 33.16796934284413 15.374989159421546 6 27.724242036205567 38.08867578479377 18.193637907776697 15.99344427122397 7 25.609209260739398 36.933294498557245 20.44786431309861 17.00963192760475 8 26.441396865134116 34.031874274822655 19.57357370782118 19.95315515222205 9 27.961471506661876 16.89834790795971 20.580230439406357 34.55995014597205 10-11 24.219147146968147 28.708054083362995 25.722177761988092 21.350621007680765 12-13 26.77304556211976 26.943972938812944 27.086079392667635 19.19690210639967 14-15 23.472130128260268 26.744097840023134 28.179025243641682 21.604746788074916 16-17 19.508931519653746 30.622048297431714 28.522984544889585 21.346035638024958 18-19 19.389193697887226 27.330908332320185 33.98200586484045 19.29789210495213 20-21 21.361547507437855 25.81619962173967 34.223753447967525 18.598499422854946 22-23 20.460137107799596 26.473563417691125 33.9365679160434 19.129731558465878 24-25 19.66465304485497 26.748324823370567 34.48885688290802 19.09816524886645 26-27 19.572640830115112 26.796486182021894 34.397982968767046 19.23289001909595 28-29 19.214205399840147 27.080347345462428 34.539943373477655 19.165503881219774 30-31 19.438287645064882 27.12032493449557 34.35819205059354 19.083195369846013 32-33 19.114724859960084 27.2288946067316 34.11006278977061 19.546317743537706 34-35 19.641642710249734 27.221985894389455 33.99889723713195 19.13747415822886 36-37 19.762979450790514 27.057469574821404 33.84596001247726 19.333590961910822 38-39 19.630684021196846 27.1915331817854 33.82846593100562 19.349316866012142 40-41 19.667334521408204 27.201474179011033 33.87591310822667 19.255278191354098 42-43 19.422221213913797 27.376939437045607 33.51531419210285 19.68552515693775 44-45 19.48094383206437 27.6927812138331 33.12371627611212 19.70255867799041 46-47 19.735972815053984 27.923558424099383 32.38879078122857 19.95167797961806 48-49 19.785668911020903 28.080840095517118 31.941752438381844 20.19173855508014 50-51 20.145673146596852 28.383882025812273 31.58481764953659 19.88562717805429 52-53 20.064743546552748 28.664983465374462 31.339573841061057 19.93069914701173 54-55 19.69175397877423 28.61142006815677 31.411129563888085 20.285696389180917 56-57 20.011600260311738 28.840563123903372 30.862414682557148 20.285421933227738 58-59 20.0297001644479 28.359153944836653 31.206266152342344 20.404879738373108 60-61 19.998388318969283 28.381533656707873 31.418096469267343 20.201981555055497 62-63 20.239137604005624 28.27503871748459 31.39017947170864 20.09564420680114 64-65 19.899010727697693 28.348541316855957 31.445295107339614 20.30715284810673 66-67 19.786083220331236 28.545971961936488 31.214277370631642 20.453667447100628 68-69 20.018636034587722 28.522071714874087 31.143915287304402 20.315376963233785 70-71 19.984822708100243 28.57570495507088 30.894153119302594 20.545319217526284 72-73 19.967716397320604 28.60305526001946 30.738127632312317 20.691100710347616 74-75 19.9410627996423 28.834227865451446 30.626553975145875 20.59815535976038 76-77 20.017315765201747 28.555606495341944 30.7574919295803 20.66958580987601 78-79 20.057119929844404 28.60709648257029 30.637308760815795 20.69847482676951 80-81 20.0110788917316 28.908932322598908 30.36242738788717 20.717561397782326 82-83 20.08467147056324 28.879655517206704 30.174174829013133 20.861498183216927 84-85 20.191449185416626 28.53433906455214 30.175390125455692 21.098821624575542 86-87 20.278779855666926 28.48125244406374 30.20136545926391 21.03860224100542 88-89 20.441200325510625 28.285830297616005 30.227887916247724 21.045081460625642 90-91 20.279029981551062 28.48308305813446 30.206812289537204 21.031074670777265 92-93 20.401011637762167 28.380170735035627 30.108955242580077 21.10986238462213 94-95 20.176099265801522 28.275412478664897 30.111518246593157 21.436970008940424 96-97 20.40819643763594 28.01121150110107 29.92771175727784 21.652880303985146 98-99 20.45764158779749 27.928239404259326 29.639324410948912 21.974794596994272 100-101 21.180937105762837 27.099993966752056 30.0437783316917 21.675290595793403 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10220.0 1 6273.5 2 3538.5 3 4268.5 4 2931.5 5 2429.0 6 4542.0 7 9813.0 8 15138.5 9 15888.0 10 11296.0 11 6896.5 12 6005.0 13 5975.5 14 5804.5 15 5484.5 16 5288.5 17 5328.0 18 5488.0 19 5656.0 20 5906.0 21 6400.0 22 7167.5 23 8140.0 24 9289.5 25 10853.0 26 12803.0 27 14888.5 28 16569.0 29 18496.0 30 20900.5 31 23038.0 32 25738.5 33 28362.0 34 30637.5 35 32954.5 36 35847.5 37 38607.5 38 40063.5 39 40894.5 40 41419.5 41 42160.5 42 42908.0 43 44167.0 44 46030.0 45 47677.0 46 48099.5 47 48673.0 48 52917.0 49 53487.5 50 49139.0 51 45347.5 52 41572.0 53 39193.5 54 37240.5 55 34652.0 56 31307.5 57 28018.0 58 25651.0 59 22792.0 60 19461.0 61 16943.0 62 14261.0 63 10696.0 64 7657.0 65 5796.5 66 4426.0 67 3367.5 68 2573.5 69 1875.0 70 1416.0 71 1119.0 72 883.0 73 789.5 74 650.0 75 535.5 76 424.0 77 281.0 78 186.5 79 108.0 80 59.5 81 36.5 82 25.5 83 17.0 84 11.0 85 8.0 86 8.0 87 7.5 88 6.0 89 2.5 90 0.5 91 2.5 92 3.5 93 2.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38766172603928895 2 0.024241554222194837 3 0.0452328441911345 4 0.03724261123521553 5 0.058707898074421574 6 0.0570150521091845 7 0.03135150727619053 8 0.029794088988172426 9 0.033382922434475014 10-11 0.03064051197079096 12-13 0.030132658181219837 14-15 0.02383527119053794 16-17 0.02915080752138234 18-19 0.025561974075079753 20-21 0.03866460184601467 22-23 0.025291118720641822 24-25 0.04672254864054312 26-27 0.03886774336184312 28-29 0.03135150727619053 30-31 0.03839374649157674 32-33 0.035719049866502164 34-35 0.0346017715294457 36-37 0.03504191148040734 38-39 0.01777488263498923 40-41 0.012493203223449574 42-43 0.012357775546230609 44-45 0.009615365082546555 46-47 0.016962316571675438 48-49 0.0141521922693819 50-51 0.009547651243937073 52-53 0.003622690365607329 54-55 0.004096687235873708 56-57 0.007583949924262072 58-59 0.0029455519795125013 60-61 0.005687962443196554 62-63 0.005417107088758623 64-65 0.0075500930049573305 66-67 0.004096687235873708 68-69 0.0063651008292913815 70-71 0.01726702884541811 72-73 0.01367819539911552 74-75 0.009920077356289228 76-77 0.007719377601481037 78-79 0.005789533201110778 80-81 0.008193374471747415 82-83 0.01046178806516509 84-85 0.004502970267530605 86-87 7.109953053995692E-4 88-89 0.023936841948452164 90-91 0.019975582389797422 92-93 0.012696344739278023 94-95 0.024580123415242253 96-97 0.01919687324578837 98-99 0.01723317192611337 100-101 0.11132155067398969 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1476803.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.928102143746955 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0318988761244 39.712960077548054 2 13.938592932776736 14.754865409722331 3 4.867480096091022 7.728794511256826 4 2.1600363102645095 4.57306489855529 5 1.1614298968051142 3.0736140105451293 6 0.6995348254838205 2.221503041778951 7 0.43567527780384274 1.6141625923574987 8 0.2852982262724698 1.208023492926329 9 0.22084390021321915 1.0519963657477853 >10 1.087668542165517 10.321151905005562 >50 0.07041467905506592 2.5245199712440254 >100 0.03637126092921616 3.5078750422180525 >500 0.003084438496301421 1.0934581763740319 >1k 0.0011566644361130328 1.8285069511110636 >5k 1.2851827067922588E-4 0.6338996420298353 >10k+ 3.8555481203767763E-4 4.15160391157924 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26211 1.7748474237931533 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20627 1.3967333489978013 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14195 0.9611979390616081 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9319 0.63102526200177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4650 0.31486934953409496 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4109 0.27823616284636477 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4041 0.27363162182091993 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3560 0.2410612654497587 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2635 0.17842596473598712 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2292 0.15520011809293455 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2214 0.14991843868139487 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1906 0.12906257638967417 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.385691930474139E-5 0.0 18-19 0.0 0.0 0.0 1.3542767721896556E-4 0.0 20-21 0.0 0.0 0.0 4.062830316568967E-4 0.0 22-23 0.0 0.0 0.0 0.0011849921756659487 0.0 24-25 0.0 0.0 0.0 0.0031825504146456907 0.0 26-27 0.0 0.0 0.0 0.006195816232767675 0.0 28-29 0.0 0.0 0.0 0.014727759897562503 0.0 30-31 0.0 0.0 0.0 0.03328135167656079 0.0 32-33 0.0 0.0 0.0 0.06354943753499959 0.0 34-35 0.0 0.0 0.0 0.10681858040645908 0.0 36-37 0.0 0.0 0.0 0.1743292775001134 0.0 38-39 0.0 0.0 0.0 0.2677405178618949 0.0 40-41 0.0 0.0 0.0 0.3741528152366971 0.0 42-43 0.0 0.0 0.0 0.4721347397046187 0.0 44-45 0.0 0.0 0.0 0.5861648439229876 0.0 46-47 0.0 0.0 0.0 0.7176989754219081 0.0 48-49 0.0 0.0 0.0 0.8639947237376956 0.0 50-51 0.0 0.0 0.0 1.018686988041059 0.0 52-53 0.0 0.0 0.0 1.1656260178236366 0.0 54-55 0.0 0.0 0.0 1.3115831969463767 0.0 56-57 0.0 0.0 0.0 1.4870636097028513 0.0 58-59 0.0 0.0 0.0 1.679269340595868 0.0 60-61 0.0 0.0 0.0 1.8869138266918473 0.0 62-63 0.0 0.0 0.0 2.085653943010679 0.0 64-65 0.0 0.0 0.0 2.2846310577646443 0.0 66-67 0.0 0.0 0.0 2.499520924591838 0.0 68-69 0.0 0.0 0.0 2.7370610704339033 0.0 70-71 0.0 3.385691930474139E-5 0.0 3.004327591425532 0.0 72-73 0.0 6.771383860948278E-5 0.0 3.27694350566731 0.0 74-75 0.0 6.771383860948278E-5 0.0 3.5351363722852676 0.0 76-77 0.0 6.771383860948278E-5 0.0 3.802707605550639 0.0 78-79 0.0 6.771383860948278E-5 0.0 4.09705966198606 0.0 80-81 0.0 6.771383860948278E-5 0.0 4.409254314895081 0.0 82-83 0.0 6.771383860948278E-5 0.0 4.721685966239234 0.0 84-85 0.0 6.771383860948278E-5 0.0 5.040076435381023 0.0 86-87 0.0 6.771383860948278E-5 0.0 5.359550325940562 0.0 88-89 0.0 6.771383860948278E-5 0.0 5.687420732487677 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11165 0.0 40.326565 1 GTATCAA 18040 0.0 35.42797 1 TCGCGAT 65 3.046771E-7 29.222914 50-51 TATCAAC 22205 0.0 28.746473 2 ATCAACG 22450 0.0 28.348076 3 TCAACGC 22750 0.0 27.974257 4 CAACGCA 22940 0.0 27.742561 5 AACGCAG 23705 0.0 26.82085 6 ATCGCGT 75 1.0597832E-6 25.33682 24-25 ACGCAGA 26000 0.0 24.428495 7 GAGTACT 14200 0.0 24.386854 12-13 CGCAGAG 26040 0.0 24.281513 8 CATTCCG 200 2.0550942E-8 23.750051 9 GCAGAGT 26375 0.0 23.736544 9 AGTACTT 14475 0.0 23.119532 12-13 CCGACCA 185 2.5426016E-7 23.108158 9 GTACATG 14245 0.0 22.901907 1 GTACTTT 15010 0.0 22.849312 14-15 TACATGG 14290 0.0 22.484013 2 TTCGCGA 85 3.1563395E-6 22.349203 48-49 >>END_MODULE