FastQCFastQC Report
Thu 26 May 2016
SRR938195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476803
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248151.6803189050943153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209331.4174537836123031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141760.9599113761280279No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92350.6253372995585734No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48300.3270578404838018No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43900.2972637514956294No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39210.26550596118778197No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.2225753875093699No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25910.1754465558371699No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24270.1643414863052147No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21800.14761616816867246No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20310.13752680621585953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116750.040.2880481
GTATCAA179200.032.2459451
TATCACG908.883646E-426.4219152
TATCAAC224000.025.839222
ATCAACG225200.025.7165473
TCAACGC227850.025.4374584
CAACGCA230450.025.1710975
ATAACGC1151.273204E-424.8077373
AACGCAG235600.024.6143656
GGACCGA1601.8618448E-623.747526
GAGTACT142900.023.73090212-13
GTACATG143350.023.2240071
TACATGG144100.022.7730882
GTACTTT150650.022.38398714-15
ACGCAGA262000.022.0978837
AGTACTT144450.022.04598412-13
CGCAGAG263200.021.9249528
CTGTGCG2405.9390004E-921.7685599
GTATAGC5750.021.505141
ACATGGG146000.021.3315413
Produced by FastQC (version 0.11.5)