##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938195_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1476803 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49450265201249 33.0 31.0 34.0 31.0 34.0 2 32.685636472840315 34.0 31.0 34.0 31.0 34.0 3 32.72013125650476 34.0 31.0 34.0 31.0 34.0 4 36.201059315291204 37.0 35.0 37.0 35.0 37.0 5 36.1526012609671 37.0 35.0 37.0 35.0 37.0 6 36.233656080059426 37.0 37.0 37.0 35.0 37.0 7 36.21029616001593 37.0 37.0 37.0 35.0 37.0 8 36.219585821534764 37.0 37.0 37.0 35.0 37.0 9 37.884403674694596 39.0 38.0 39.0 35.0 39.0 10-11 37.87485974771178 39.0 38.0 39.0 35.0 39.0 12-13 37.7920013705281 39.0 38.0 39.0 35.0 39.0 14-15 38.928028315218754 40.0 38.0 41.0 35.5 41.0 16-17 38.86437324409552 40.0 38.0 41.0 35.5 41.0 18-19 39.189460273306594 40.0 38.5 41.0 36.0 41.0 20-21 39.20745454877868 41.0 39.0 41.0 36.0 41.0 22-23 39.079695463782244 40.0 39.0 41.0 35.0 41.0 24-25 38.96415466382449 40.0 38.0 41.0 35.0 41.0 26-27 38.77926913745435 40.0 38.0 41.0 35.0 41.0 28-29 38.6295728001636 40.0 38.0 41.0 35.0 41.0 30-31 38.291394993103346 40.0 37.5 41.0 34.0 41.0 32-33 37.933612675488874 40.0 36.5 41.0 34.0 41.0 34-35 37.7499998307154 40.0 36.0 41.0 33.0 41.0 36-37 37.64591113371249 40.0 36.0 41.0 33.0 41.0 38-39 37.43687275824873 40.0 35.0 41.0 33.0 41.0 40-41 37.17585994882188 40.0 35.0 41.0 33.0 41.0 42-43 36.8456842246393 40.0 35.0 41.0 32.0 41.0 44-45 36.42551951749827 39.0 35.0 41.0 31.0 41.0 46-47 35.82632179105812 39.0 35.0 41.0 29.5 41.0 48-49 35.25035702121407 39.0 35.0 40.5 26.5 41.0 50-51 34.82977621253478 38.5 35.0 40.0 24.0 41.0 52-53 34.32882788022505 38.0 34.0 40.0 23.0 41.0 54-55 34.010556926008405 38.0 34.0 40.0 22.0 41.0 56-57 33.68891280692144 38.0 33.5 40.0 20.0 41.0 58-59 33.246744487924246 37.0 33.0 40.0 18.5 41.0 60-61 32.83366908111644 36.5 33.0 40.0 14.0 41.0 62-63 32.33724098610309 36.0 32.0 39.0 9.5 40.5 64-65 31.82943832048012 35.0 31.0 39.0 7.5 40.0 66-67 31.331157574842415 35.0 31.0 38.5 2.0 40.0 68-69 30.810719845504106 35.0 30.0 38.0 2.0 40.0 70-71 30.270750059418894 35.0 29.5 37.0 2.0 39.5 72-73 29.656919372455228 34.0 29.0 36.5 2.0 39.0 74-75 29.06107720528737 34.0 28.0 36.0 2.0 39.0 76-77 27.719399608478582 32.5 26.5 35.0 2.0 37.0 78-79 28.40072474121464 34.0 28.0 35.0 2.0 37.0 80-81 28.15383974707527 34.0 27.0 35.0 2.0 37.0 82-83 27.65414276650305 33.5 26.0 35.0 2.0 36.5 84-85 27.054594282378897 33.0 25.5 35.0 2.0 36.0 86-87 26.398208833541105 33.0 24.5 35.0 2.0 35.5 88-89 25.6635241802732 32.0 21.5 35.0 2.0 35.0 90-91 25.06283099370735 32.0 19.0 34.5 2.0 35.0 92-93 24.20156920049594 31.0 10.0 34.0 2.0 35.0 94-95 23.390749477079883 31.0 2.0 34.0 2.0 35.0 96-97 22.435547936996336 30.5 2.0 34.0 2.0 35.0 98-99 21.12759454036862 29.5 2.0 34.0 2.0 35.0 100-101 17.301570690200386 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11392557591203456 1101 2 0.11365111058171351 1101 3 0.12016822768602964 1101 4 0.013425774633084586 1101 5 0.050569818258125565 1101 6 0.05262369516660925 1101 7 0.08882362991631254 1101 8 0.014891243092748141 1101 9 -0.019321124918995736 1101 10-11 0.04875443816340663 1101 12-13 0.13082122800335583 1101 14-15 0.2817427446683709 1101 16-17 0.20029454950222458 1101 18-19 0.155231073362593 1101 20-21 0.08112545017445427 1101 22-23 0.044272424381468056 1101 24-25 0.28908909967810814 1101 26-27 0.22598562060868943 1101 28-29 0.24374494797466184 1101 30-31 0.33105260793286817 1101 32-33 0.20308683616058687 1101 34-35 0.24665440231920854 1101 36-37 0.4012763037399978 1101 38-39 0.45046260615440303 1101 40-41 0.6450555062664662 1101 42-43 0.5732104259987807 1101 44-45 0.5501856941660392 1101 46-47 0.6784086497438366 1101 48-49 0.6501528505034955 1101 50-51 0.6519519112618539 1101 52-53 0.7766276017552585 1101 54-55 0.7451949603821504 1101 56-57 0.8916024839164649 1101 58-59 0.8757897574345392 1101 60-61 0.8917513115725129 1101 62-63 0.9016820633882503 1101 64-65 0.9572991200549836 1101 66-67 0.9230913715827107 1101 68-69 1.0710058452333975 1101 70-71 1.0762123017054144 1101 72-73 1.2148266903486018 1101 74-75 1.2816459625945953 1101 76-77 1.055355450200576 1101 78-79 1.0577826874409126 1101 80-81 1.072818083931228 1101 82-83 1.1519160102185886 1101 84-85 1.4874282575872577 1101 86-87 1.5825694006535151 1101 88-89 1.8501571842103388 1101 90-91 2.0726774439102265 1101 92-93 2.1255598527211284 1101 94-95 2.4558108493973236 1101 96-97 2.7641582839498504 1101 98-99 2.9799025184819463 1101 100-101 3.166579571912525 1102 1 0.28039779093234074 1102 2 0.2781666559105531 1102 3 0.23946431272621993 1102 4 0.09023417494420727 1102 5 -0.005591598214088833 1102 6 0.08334435457374667 1102 7 0.12279730332397065 1102 8 0.15488697595939982 1102 9 0.2009075178085098 1102 10-11 0.2553498599074189 1102 12-13 0.35301869365022753 1102 14-15 0.5379543944825684 1102 16-17 0.49245814815402866 1102 18-19 0.37017306195475896 1102 20-21 0.3298034490509494 1102 22-23 0.34576767652043117 1102 24-25 0.39086765706295523 1102 26-27 0.4307884412378442 1102 28-29 0.4360198161796589 1102 30-31 0.5430508146565742 1102 32-33 0.5111259263429346 1102 34-35 0.5746308628671244 1102 36-37 0.511206301147304 1102 38-39 0.6415349799768961 1102 40-41 0.7143187775913376 1102 42-43 0.7831122009101961 1102 44-45 0.6089418247943357 1102 46-47 0.6899002019000875 1102 48-49 0.8379512797663082 1102 50-51 0.7961074239660348 1102 52-53 0.9747819315452446 1102 54-55 1.1274004741666133 1102 56-57 0.9904386097915037 1102 58-59 1.084563982868815 1102 60-61 0.927141511209534 1102 62-63 1.1299804494395858 1102 64-65 1.1920799028308906 1102 66-67 1.471993935103587 1102 68-69 1.573717365413156 1102 70-71 1.6889989828479237 1102 72-73 1.8189258390825103 1102 74-75 1.855420107007351 1102 76-77 1.8480733710803605 1102 78-79 1.6855556489394878 1102 80-81 1.9033487478755688 1102 82-83 2.0057113282550567 1102 84-85 2.198392764760367 1102 86-87 2.237777598950988 1102 88-89 2.559125861290969 1102 90-91 2.7289065133510704 1102 92-93 2.6710325052576422 1102 94-95 3.120345974926664 1102 96-97 3.33205566749492 1102 98-99 3.4732317467249665 1102 100-101 3.337853662310934 1103 1 0.19888768614433872 1103 2 0.2107779326033281 1103 3 0.2604690683005515 1103 4 0.11074864514532123 1103 5 0.05047017645101448 1103 6 0.12893249677330232 1103 7 0.04787020016766519 1103 8 0.1924341796532687 1103 9 0.15499704042946405 1103 10-11 0.2428249742104427 1103 12-13 0.2234680508531639 1103 14-15 0.3675485751375476 1103 16-17 0.3470449325408609 1103 18-19 0.2596621574654492 1103 20-21 0.20167586116455283 1103 22-23 0.2943380973906997 1103 24-25 0.29209679409609635 1103 26-27 0.2945304490694909 1103 28-29 0.36825062108742657 1103 30-31 0.2213987402778912 1103 32-33 0.36557253691423597 1103 34-35 0.45540463524810093 1103 36-37 0.42102867443857406 1103 38-39 0.37353039622853146 1103 40-41 0.41608089810464577 1103 42-43 0.6050042502772186 1103 44-45 0.7124882869234952 1103 46-47 0.9312879097545945 1103 48-49 0.9857613384326029 1103 50-51 1.0986386917703683 1103 52-53 1.1597348215159045 1103 54-55 1.2396667811614819 1103 56-57 1.2647464935027841 1103 58-59 1.526964172006572 1103 60-61 1.4685764389069789 1103 62-63 1.4316619538599085 1103 64-65 1.527505508000658 1103 66-67 1.5627383937161348 1103 68-69 1.5256261193648264 1103 70-71 1.7990997012871723 1103 72-73 2.2431909600906224 1103 74-75 2.1862997526066046 1103 76-77 1.898854081096264 1103 78-79 1.9920905909772912 1103 80-81 2.1047807816619795 1103 82-83 2.3282528926981954 1103 84-85 2.2260247703828178 1103 86-87 2.416714642174906 1103 88-89 2.655944626236927 1103 90-91 2.871766886019632 1103 92-93 3.1024335716121776 1103 94-95 3.1486543030600025 1103 96-97 3.6168119911426935 1103 98-99 3.9897864681278925 1103 100-101 3.7575124262750244 1104 1 0.1386328452242651 1104 2 0.1684625590491251 1104 3 0.1454399776760411 1104 4 0.14585394072028635 1104 5 0.050241370430128995 1104 6 0.09104210023394899 1104 7 0.16527516949989263 1104 8 0.1633328018586795 1104 9 0.08549556872285535 1104 10-11 0.09600409348050931 1104 12-13 0.1444081030782911 1104 14-15 0.2836959477215615 1104 16-17 0.20879173209424096 1104 18-19 0.2741504175659273 1104 20-21 0.23730239238761897 1104 22-23 0.3251543255324023 1104 24-25 0.3703702585119828 1104 26-27 0.35010611725177654 1104 28-29 0.3998838002305902 1104 30-31 0.23921335005012168 1104 32-33 0.4391736987470054 1104 34-35 0.45337734221449466 1104 36-37 0.5236637073286943 1104 38-39 0.574168484246556 1104 40-41 0.51089799367805 1104 42-43 0.656048902100764 1104 44-45 0.7018795732972407 1104 46-47 0.908350852916918 1104 48-49 1.0313552692782153 1104 50-51 1.0635779028495946 1104 52-53 1.0340495235871714 1104 54-55 1.121488770077228 1104 56-57 1.2811706437094585 1104 58-59 1.0775694326333607 1104 60-61 1.26879443190181 1104 62-63 1.39079020889438 1104 64-65 1.4515141297460659 1104 66-67 1.6068026696884523 1104 68-69 1.5294590682682063 1104 70-71 1.5620862351866087 1104 72-73 1.773888603388606 1104 74-75 1.6734636153540556 1104 76-77 1.6846543077516287 1104 78-79 1.8861327928439415 1104 80-81 2.0345573463098354 1104 82-83 2.152178893366095 1104 84-85 2.359270225476802 1104 86-87 2.3644229079268264 1104 88-89 2.4607907138630587 1104 90-91 2.7938193058971983 1104 92-93 3.0332875557343577 1104 94-95 3.229456283098319 1104 96-97 3.653907839358876 1104 98-99 3.4971291699625837 1104 100-101 3.129865848538028 1105 1 0.17177946784808285 1105 2 0.21492641983600436 1105 3 0.1125778826438264 1105 4 0.11790120933958548 1105 5 0.044128236198766047 1105 6 0.04236262814206526 1105 7 0.12912332941450444 1105 8 0.09675671261467045 1105 9 0.1339208179370317 1105 10-11 0.1600817274231403 1105 12-13 0.1828176443491003 1105 14-15 0.30484442903449605 1105 16-17 0.21624837530843166 1105 18-19 0.25538253988921866 1105 20-21 0.21172521385749832 1105 22-23 0.23193197479574934 1105 24-25 0.2690893792712217 1105 26-27 0.27961735272445054 1105 28-29 0.286663705511522 1105 30-31 0.35647491508656515 1105 32-33 0.34379297894270877 1105 34-35 0.4157675086715571 1105 36-37 0.4728132392050455 1105 38-39 0.49554122044234816 1105 40-41 0.5503345280309659 1105 42-43 0.5340269715894763 1105 44-45 0.5549530605448396 1105 46-47 0.6024845337442954 1105 48-49 0.6293371436921191 1105 50-51 0.8500870860197054 1105 52-53 0.7879802975190557 1105 54-55 0.7703632024951972 1105 56-57 0.6443330752923586 1105 58-59 0.8561370971669078 1105 60-61 0.7819660390786467 1105 62-63 0.8897598486218428 1105 64-65 0.9441542932568368 1105 66-67 0.7849447213512555 1105 68-69 1.2249084787573388 1105 70-71 1.2972996392982843 1105 72-73 0.9968328073595316 1105 74-75 0.9309055271100988 1105 76-77 0.9879750309567896 1105 78-79 1.2031866022306588 1105 80-81 1.3090818569307991 1105 82-83 1.3595692029287463 1105 84-85 1.45557439725739 1105 86-87 1.764376945777414 1105 88-89 1.7512167813045174 1105 90-91 1.9100092625782956 1105 92-93 2.064524981382583 1105 94-95 2.554598083905436 1105 96-97 2.7282250994086787 1105 98-99 2.981334521438338 1105 100-101 2.8481473355566678 1106 1 0.08957109346858516 1106 2 0.0662810233701947 1106 3 0.03197605768070133 1106 4 0.09075458870879771 1106 5 0.024099314030841867 1106 6 0.04442857367982356 1106 7 -0.004132564386374327 1106 8 0.07402917248569452 1106 9 0.10571100814521373 1106 10-11 0.04336917462467227 1106 12-13 0.06557563618372342 1106 14-15 0.0530600574873219 1106 16-17 0.018302394730348226 1106 18-19 -0.07715286676994282 1106 20-21 -0.1443784438768816 1106 22-23 -0.20799890721271908 1106 24-25 -0.15143081330079156 1106 26-27 -0.20828619655180347 1106 28-29 0.00447977164745339 1106 30-31 -0.050100278806411325 1106 32-33 -0.0021202673888112145 1106 34-35 -0.033047260031317194 1106 36-37 9.602095972596203E-4 1106 38-39 -0.11012309629818162 1106 40-41 -0.2065355564572684 1106 42-43 -0.11361299448259388 1106 44-45 0.10148256083524387 1106 46-47 0.09933988974687935 1106 48-49 0.22742810947219283 1106 50-51 0.3797069049780575 1106 52-53 0.32960431361450304 1106 54-55 0.40596501141421726 1106 56-57 0.43374380751308905 1106 58-59 0.6925926127548294 1106 60-61 0.6473496054258163 1106 62-63 0.6452399790710857 1106 64-65 0.925047639553803 1106 66-67 1.0017858408873046 1106 68-69 0.8985155565594702 1106 70-71 0.7745167470173193 1106 72-73 1.0019463300774483 1106 74-75 0.9745716899919579 1106 76-77 1.1378630431246357 1106 78-79 1.2231804947758214 1106 80-81 1.1637493001320003 1106 82-83 1.5423529722872935 1106 84-85 1.6142342548071547 1106 86-87 1.62209009434687 1106 88-89 1.7137457810189325 1106 90-91 1.7776857985069192 1106 92-93 1.4696353487604306 1106 94-95 1.6385271882273962 1106 96-97 2.373071519916035 1106 98-99 2.762192677671525 1106 100-101 2.645800190390702 1107 1 0.1911874673710372 1107 2 0.18896853431620997 1107 3 0.12640263004011132 1107 4 0.09476633409969537 1107 5 -0.03142141912407226 1107 6 0.06369918777286188 1107 7 0.14255577410374798 1107 8 0.02636191486240591 1107 9 0.17564770306923094 1107 10-11 0.1857838315779361 1107 12-13 0.2433099371701175 1107 14-15 0.32826097761805073 1107 16-17 0.2510683789803778 1107 18-19 0.1799905902546044 1107 20-21 0.20950164211396327 1107 22-23 0.16142733228551265 1107 24-25 0.24445276926875437 1107 26-27 0.3141376371282192 1107 28-29 0.25032156733623623 1107 30-31 0.38859744534487106 1107 32-33 0.3726719504191678 1107 34-35 0.4515870996410598 1107 36-37 0.5223885322943929 1107 38-39 0.6293556738155957 1107 40-41 0.7227076298743214 1107 42-43 0.7379631800308886 1107 44-45 0.8392156020700838 1107 46-47 0.7263038987474459 1107 48-49 1.0248810557033323 1107 50-51 1.0804864658675868 1107 52-53 1.1711211671443138 1107 54-55 1.2426062167608904 1107 56-57 1.3296184284354098 1107 58-59 1.4384367128671798 1107 60-61 1.3532647022679427 1107 62-63 1.3892208247023632 1107 64-65 1.5288524477079761 1107 66-67 1.5819641623042067 1107 68-69 1.4261432907580378 1107 70-71 1.4933081946357198 1107 72-73 1.8219301376611554 1107 74-75 1.8429732291420713 1107 76-77 1.7122890112129774 1107 78-79 1.8207377545806303 1107 80-81 1.8879315626253081 1107 82-83 1.9202356781516947 1107 84-85 1.8664408684003853 1107 86-87 2.102592878602639 1107 88-89 2.2647708398329414 1107 90-91 2.4109790348701097 1107 92-93 2.536381185179131 1107 94-95 2.729889133283791 1107 96-97 2.769008104227723 1107 98-99 3.039483197887467 1107 100-101 3.225331480878147 1108 1 0.22401455193374886 1108 2 0.2198073275927186 1108 3 0.15328917475283532 1108 4 0.08272150680017631 1108 5 0.07247922106313354 1108 6 0.12540212225151492 1108 7 0.15701423088147237 1108 8 0.23432694283637545 1108 9 0.17012286919953823 1108 10-11 0.15762989058385557 1108 12-13 0.2144789530825335 1108 14-15 0.33576928666807504 1108 16-17 0.2765650969309732 1108 18-19 0.19064711166752346 1108 20-21 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3.8514214113596275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 14.0 5 39.0 6 127.0 7 239.0 8 479.0 9 865.0 10 1591.0 11 2669.0 12 4169.0 13 6717.0 14 8212.0 15 12008.0 16 17231.0 17 26036.0 18 25840.0 19 16328.0 20 12331.0 21 12689.0 22 13495.0 23 14499.0 24 16431.0 25 18920.0 26 21245.0 27 25038.0 28 29790.0 29 36233.0 30 43629.0 31 52782.0 32 65086.0 33 83031.0 34 114005.0 35 172945.0 36 203583.0 37 260104.0 38 151485.0 39 6913.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.04114631403105 19.889314959408942 15.783147786129902 19.286390940430103 2 15.469969469660644 21.908430653869388 40.81678816495594 21.804811711514027 3 18.905745519946258 28.439267373074173 30.835446055169125 21.81954105181044 4 11.877750074825059 18.76192630171901 37.72625269336353 31.634070930092406 5 11.912316455885005 39.636782993194515 33.24648505138354 15.204415499536944 6 27.85593464797583 38.22875375304204 17.896779663082796 16.018531935899325 7 25.6930003527891 36.91873594514637 20.185359861809598 17.20290384025493 8 26.549146565167725 34.219598225077654 19.4336797813105 19.797575428444127 9 27.790030220686173 16.883836232727045 20.555889986680686 34.77024355990609 10-11 24.32033927341697 28.756475982240016 25.553882271365918 21.3693024729771 12-13 26.71205976694251 26.91215416003353 27.038135756766472 19.33765031625748 14-15 23.382604179433546 26.841901052476192 28.05408033434385 21.72141443374641 16-17 19.524168084707302 30.649856480519066 28.52425137272879 21.301724062044837 18-19 19.369069537372283 27.376095525266404 33.867516520483775 19.387318416877537 20-21 21.261739040345937 25.875387577083742 34.18299529456535 18.679878088004966 22-23 20.440167781633335 26.535202283585356 33.8996702669047 19.124959667876603 24-25 19.684120430905534 26.72655754311527 34.51466247561446 19.074659550364743 26-27 19.632523554728195 26.864325375045585 34.363645659418964 19.13950541080726 28-29 19.266686213394745 27.145123621769457 34.47287823765255 19.115311927183246 30-31 19.396764497363563 27.155991692866277 34.32455107417848 19.12269273559168 32-33 19.1285499826314 27.203154381457782 34.070116325603344 19.59817931030747 34-35 19.65132587185462 27.199825822079827 33.962391926054295 19.186456380011254 36-37 19.707291506903303 27.07436754021466 33.92034837639792 19.29799257648412 38-39 19.546749499394263 27.202783753896327 33.86420904293392 19.386257703775485 40-41 19.62275216136865 27.217212258540087 33.90127845213736 19.258757127953903 42-43 19.42245789411491 27.361926589507107 33.54175646287202 19.673859053505954 44-45 19.43443468015121 27.67565993624524 33.172315604679866 19.717589778923685 46-47 19.714145933528687 27.922115516462085 32.32737620636477 20.036362343644463 48-49 19.763739645707652 28.05187963458904 32.00003656547285 20.184344154230455 50-51 20.090865200030063 28.417331221564417 31.58359645802453 19.908207120380986 52-53 20.06320409695809 28.626803981302857 31.315077231018627 19.994914690720428 54-55 19.651707099728263 28.62876768262253 31.33620394866479 20.38332126898442 56-57 19.965736786063133 28.88483734284036 30.82199549364251 20.327430377453993 58-59 19.90008145974785 28.50373407963012 31.08190462776687 20.514279832855163 60-61 19.90597256370687 28.582349846255727 31.2377818842459 20.273895705791496 62-63 20.099295572936946 28.57493518092799 31.16651306910942 20.159256177025643 64-65 19.858911445873282 28.584990685961497 31.16813820123605 20.38795966692917 66-67 19.683417168793504 28.74360569108306 31.051498796554583 20.521478343568855 68-69 19.93010532616781 28.781342821457436 30.903643603523445 20.384908248851314 70-71 19.872528655659778 28.792949632736942 30.60558199217566 20.728939719427615 72-73 19.83569903074346 28.824514051308164 30.46779459941143 20.871992318536947 74-75 19.86419312528482 29.01964581599577 30.274146246994356 20.842014811725058 76-77 19.78185309753569 28.73558626302899 30.806207733868362 20.676352905566958 78-79 19.75280386077222 28.82567952529891 30.718078172918123 20.703438441010753 80-81 19.69129250526052 29.206850611371525 30.40176326895438 20.70009361441357 82-83 19.741233781403693 29.21663189499836 30.170770536106673 20.871363787491273 84-85 19.80717596589774 28.89252303222988 30.201288653685328 21.09901234818705 86-87 19.8410027391138 28.918100835954508 30.24814542696656 20.992750997965132 88-89 19.903941148548586 28.844842541625386 30.01977921225783 21.231437097568193 90-91 19.70743532749739 28.904956264273153 30.362057669876947 21.025550738352518 92-93 19.76969846350529 28.87521219824174 30.211544803199885 21.143544535053085 94-95 19.539640696829572 28.787488920323156 30.139869705031746 21.533000677815526 96-97 19.6757449635208 28.667920954793825 29.941281845448998 21.715052236236374 98-99 19.63867218579594 28.64166716887764 29.806886903669614 21.912773741656807 100-101 20.003108065192173 28.31071578267379 30.02492546399215 21.661250688141884 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9962.0 1 6110.5 2 3476.5 3 4272.5 4 2945.5 5 2417.5 6 4708.0 7 10024.0 8 14892.5 9 15812.5 10 11612.0 11 6881.5 12 5793.0 13 5778.5 14 5638.5 15 5441.0 16 5332.5 17 5314.5 18 5470.5 19 5638.5 20 5738.5 21 6137.5 22 6883.5 23 7933.5 24 8955.5 25 10390.5 26 12500.5 27 14510.0 28 16030.0 29 18354.5 30 20863.0 31 22810.5 32 25638.0 33 28414.5 34 30992.5 35 33536.0 36 36291.0 37 38924.0 38 40517.5 39 41472.5 40 42012.5 41 42841.5 42 43856.5 43 45314.5 44 47440.0 45 48982.0 46 48913.5 47 49439.5 48 55088.5 49 55347.5 50 49853.5 51 45962.5 52 41716.5 53 39147.0 54 37162.5 55 34440.5 56 31085.5 57 27857.5 58 25187.0 59 22407.5 60 18960.5 61 15902.5 62 13101.5 63 9503.5 64 6416.5 65 4804.0 66 3591.0 67 2632.5 68 1975.5 69 1451.0 70 1088.0 71 865.5 72 712.5 73 662.0 74 531.0 75 408.5 76 298.5 77 170.5 78 102.5 79 57.5 80 28.0 81 14.0 82 9.0 83 10.0 84 8.5 85 3.5 86 0.5 87 2.0 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016183607427666384 3 0.0023699843513318975 4 0.0016928459652370694 5 0.12303604475343022 6 6.771383860948278E-5 7 0.0 8 4.062830316568967E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.6928459652370696E-4 24-25 4.062830316568967E-4 26-27 0.00558639168528233 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010292503468641382 36-37 6.432814667900864E-4 38-39 0.006229673152072416 40-41 0.009107511292975434 42-43 0.0053832501694538815 44-45 0.00341954884977888 46-47 3.385691930474139E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.385691930474139E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.0157075791422417E-4 68-69 6.432814667900864E-4 70-71 3.385691930474139E-5 72-73 6.771383860948278E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.385691930474139E-5 82-83 0.0017605598038465521 84-85 0.004604541025444829 86-87 0.0032502642532551734 88-89 0.0 90-91 0.002403841270636639 92-93 0.0 94-95 0.0 96-97 1.6928459652370696E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1476803.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.55935572820436 #Duplication Level Percentage of deduplicated Percentage of total 1 74.12016470694176 38.21587938758308 2 14.312845446127254 14.759221796793698 3 4.947812984762478 7.653181492739915 4 2.312755810354161 4.769767981540869 5 1.2331422275025559 3.1790009385637292 6 0.7530082944685472 2.329477351247537 7 0.46865998618009 1.6914664860124222 8 0.32329643430494603 1.333516468959101 9 0.23942681862691106 1.1110223261211456 >10 1.170474575547311 10.89436495462212 >50 0.07539109255611672 2.6845501373300142 >100 0.037775145243576155 3.6127580646927253 >500 0.0035413722343767898 1.2569014535761833 >1k 0.0011804574114589299 1.831927444093685 >5k 1.3116193460654778E-4 0.6245284043783521 >10k+ 3.934858038196433E-4 4.052435311745357 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24815 1.6803189050943153 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20933 1.4174537836123031 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14176 0.9599113761280279 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9235 0.6253372995585734 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4830 0.3270578404838018 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4390 0.2972637514956294 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3921 0.26550596118778197 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3287 0.2225753875093699 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2591 0.1754465558371699 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2427 0.1643414863052147 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2180 0.14761616816867246 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2031 0.13752680621585953 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.385691930474139E-5 0.0 18-19 0.0 0.0 0.0 1.3542767721896556E-4 0.0 20-21 0.0 0.0 0.0 4.401399509616381E-4 0.0 22-23 0.0 0.0 0.0 0.0012527060142754314 0.0 24-25 0.0 0.0 0.0 0.0031825504146456907 0.0 26-27 0.0 0.0 0.0 0.006161959313462933 0.0 28-29 0.0 0.0 0.0 0.014829330655476728 0.0 30-31 0.0 0.0 0.0 0.03368763470821769 0.0 32-33 0.0 0.0 0.0 0.06432814667900864 0.0 34-35 0.0 0.0 0.0 0.10780043106629658 0.0 36-37 0.0 0.0 0.0 0.17564969735299835 0.0 38-39 0.0 0.0 0.0 0.26967036226226515 0.0 40-41 0.0 0.0 0.0 0.37679365494246697 0.0 42-43 0.0 0.0 0.0 0.47514800552274067 0.0 44-45 0.0 0.0 0.0 0.5901261034816424 0.0 46-47 0.0 0.0 0.0 0.7230145117527524 0.0 48-49 0.0 0.0 0.0 0.8704613953249012 0.0 50-51 0.0 0.0 0.0 1.0264402225618448 0.0 52-53 0.0 0.0 0.0 1.174631958358698 0.0 54-55 0.0 0.0 0.0 1.3215032743026658 0.0 56-57 0.0 0.0 0.0 1.4981686792348066 0.0 58-59 0.0 0.0 0.0 1.6924058252861078 0.0 60-61 0.0 0.0 0.0 1.9022171542175903 0.0 62-63 0.0 0.0 0.0 2.102311547308612 0.0 64-65 0.0 0.0 0.0 2.3029476511085094 0.0 66-67 0.0 0.0 0.0 2.521832634413663 0.0 68-69 0.0 0.0 0.0 2.7629954706213358 0.0 70-71 0.0 0.0 0.0 3.03601766789477 0.0 72-73 0.0 0.0 0.0 3.3155403936747145 0.0 74-75 0.0 0.0 0.0 3.5821636331995537 0.0 76-77 0.0 0.0 0.0 3.859451802305385 0.0 78-79 0.0 0.0 0.0 4.164942785192067 0.0 80-81 0.0 0.0 0.0 4.490917204258118 0.0 82-83 0.0 0.0 0.0 4.819871032222984 0.0 84-85 6.771383860948278E-5 0.0 0.0 5.159760645123283 0.0 86-87 6.771383860948278E-5 0.0 0.0 5.499413259588449 0.0 88-89 6.771383860948278E-5 0.0 0.0 5.85403063238631 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11675 0.0 40.288048 1 GTATCAA 17920 0.0 32.245945 1 TATCACG 90 8.883646E-4 26.421915 2 TATCAAC 22400 0.0 25.83922 2 ATCAACG 22520 0.0 25.716547 3 TCAACGC 22785 0.0 25.437458 4 CAACGCA 23045 0.0 25.171097 5 ATAACGC 115 1.273204E-4 24.807737 3 AACGCAG 23560 0.0 24.614365 6 GGACCGA 160 1.8618448E-6 23.74752 6 GAGTACT 14290 0.0 23.730902 12-13 GTACATG 14335 0.0 23.224007 1 TACATGG 14410 0.0 22.773088 2 GTACTTT 15065 0.0 22.383987 14-15 ACGCAGA 26200 0.0 22.097883 7 AGTACTT 14445 0.0 22.045984 12-13 CGCAGAG 26320 0.0 21.924952 8 CTGTGCG 240 5.9390004E-9 21.768559 9 GTATAGC 575 0.0 21.50514 1 ACATGGG 14600 0.0 21.331541 3 >>END_MODULE