FastQCFastQC Report
Thu 26 May 2016
SRR938194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474725
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262731.7815524928376476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205691.3947685161640306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144470.9796402719151028No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94850.6431707606502907No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45810.31063418603468446No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41780.2833070572479615No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41420.280865924155351No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35780.24262150570445337No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26520.17983013782230586No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23800.16138602112258219No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21940.14877350014409466No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19070.12931224465578328No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107900.041.542031
GTATCAA173450.035.1997681
TATCAAC215200.028.2456052
ATCAACG217900.027.786563
TCAACGC220450.027.4867084
CAACGCA223250.027.1845445
AACGCAG228550.026.614726
GTACATG138400.024.661161
GAGTACT142250.024.1660512-13
ACGCAGA253700.023.9885437
TACATGG140150.023.8727342
CGCAGAG254250.023.8619088
GTGTAAG5900.023.4633261
GCAGAGT258650.022.8682069
GTACTTT150550.022.61205914-15
ACATGGG141450.022.4773853
AGTACTT144500.021.94839912-13
CATTCCG1754.0439336E-621.7183659
GTATAGC6950.021.2921961
ACTTTTT166650.020.76681716-17
Produced by FastQC (version 0.11.5)