##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938194_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1474725 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93964366237773 33.0 31.0 34.0 30.0 34.0 2 32.101425011442814 33.0 31.0 34.0 30.0 34.0 3 32.137428334096185 34.0 31.0 34.0 30.0 34.0 4 35.689822170235125 37.0 35.0 37.0 35.0 37.0 5 35.72827611927647 37.0 35.0 37.0 35.0 37.0 6 35.79101120547899 37.0 36.0 37.0 35.0 37.0 7 35.74290596551899 37.0 36.0 37.0 35.0 37.0 8 35.73451050195799 37.0 36.0 37.0 35.0 37.0 9 37.35438200342437 39.0 37.0 39.0 35.0 39.0 10-11 37.28045974673245 39.0 37.0 39.0 34.0 39.0 12-13 37.16827035549001 39.0 37.0 39.0 34.0 39.0 14-15 38.255178762142094 40.0 38.0 41.0 33.5 41.0 16-17 38.13382969706217 40.0 38.0 41.0 33.0 41.0 18-19 38.382806624963976 40.0 38.0 41.0 34.0 41.0 20-21 38.41401413822916 40.0 38.0 41.0 34.0 41.0 22-23 38.30532641678957 40.0 38.0 41.0 34.0 41.0 24-25 38.189667226093 40.0 38.0 41.0 34.0 41.0 26-27 37.99459933207886 40.0 38.0 41.0 33.0 41.0 28-29 37.75683771550628 40.0 37.5 41.0 33.0 41.0 30-31 37.43627388157114 40.0 36.5 41.0 33.0 41.0 32-33 37.06181186322874 39.0 35.5 41.0 32.0 41.0 34-35 36.7867256607164 39.0 35.0 41.0 31.0 41.0 36-37 36.50347522419435 39.0 35.0 41.0 31.0 41.0 38-39 36.19593517435454 39.0 35.0 40.0 30.0 41.0 40-41 35.90771228534133 38.5 35.0 40.0 30.0 41.0 42-43 35.604424553730354 38.5 35.0 40.0 28.5 41.0 44-45 35.17346081472817 38.0 35.0 40.0 26.0 41.0 46-47 34.57645323704419 38.0 34.5 40.0 23.5 41.0 48-49 34.027639729441084 38.0 34.0 40.0 20.0 41.0 50-51 33.26747020630965 37.5 33.0 39.5 17.0 40.5 52-53 33.382384173320446 38.0 33.0 40.0 16.0 40.5 54-55 33.498522775432704 38.0 34.0 40.0 14.0 41.0 56-57 33.23510620624184 38.0 33.0 40.0 9.0 41.0 58-59 32.95201681669464 37.0 33.0 40.0 7.5 41.0 60-61 32.56818525487803 37.0 33.0 40.0 4.0 41.0 62-63 32.06887792639306 36.0 32.0 39.5 2.0 41.0 64-65 31.589632643374188 35.5 31.0 39.0 2.0 40.5 66-67 31.098781806777534 35.0 31.0 39.0 2.0 40.0 68-69 30.541465018901828 35.0 30.0 38.0 2.0 40.0 70-71 30.048560579091017 35.0 29.0 37.5 2.0 40.0 72-73 29.45463120242757 34.5 29.0 37.0 2.0 39.0 74-75 28.812197867399007 34.0 27.5 36.0 2.0 39.0 76-77 28.187232873925648 34.0 26.5 36.0 2.0 38.5 78-79 27.500150875586975 33.5 26.0 35.0 2.0 37.5 80-81 26.865824136703452 33.0 24.5 35.0 2.0 37.0 82-83 26.17275831087152 32.5 22.5 35.0 2.0 36.0 84-85 25.53410195121124 32.0 20.0 35.0 2.0 36.0 86-87 24.86329281730492 31.5 16.5 35.0 2.0 35.0 88-89 24.077923341639966 31.0 7.5 34.0 2.0 35.0 90-91 23.271036972994963 31.0 2.0 34.0 2.0 35.0 92-93 22.409168149994066 30.0 2.0 34.0 2.0 35.0 94-95 21.503078540066795 29.0 2.0 34.0 2.0 35.0 96-97 20.343975656478328 29.0 2.0 34.0 2.0 35.0 98-99 18.931809320381767 26.0 2.0 33.5 2.0 35.0 100-101 15.373089559070335 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4620688885343718 1101 2 0.3676414988803387 1101 3 0.3130942704773716 1101 4 0.2377333265092858 1101 5 0.20019419446722964 1101 6 0.26875193782226603 1101 7 0.20887233029639418 1101 8 0.2367538148067112 1101 9 0.34072481518425235 1101 10-11 0.3924118263291021 1101 12-13 0.45814192114239916 1101 14-15 0.7041585674665569 1101 16-17 0.8112475833370993 1101 18-19 0.5811918612050562 1101 20-21 0.6431764128256603 1101 22-23 0.5536268199384224 1101 24-25 0.5571284992676766 1101 26-27 0.5490686608636182 1101 28-29 0.7281966784360847 1101 30-31 0.7272891561103378 1101 32-33 0.556271467768461 1101 34-35 0.7306023392447258 1101 36-37 0.6316409620781869 1101 38-39 0.7016552527573907 1101 40-41 0.6003068918370289 1101 42-43 0.9296005558523674 1101 44-45 0.77637315024554 1101 46-47 0.9633121271189324 1101 48-49 0.844271180293866 1101 50-51 0.7945444863591007 1101 52-53 0.8517568544255099 1101 54-55 0.8156462948419687 1101 56-57 1.0037701719767185 1101 58-59 0.960711333480397 1101 60-61 1.0137663393153886 1101 62-63 0.9076938826083989 1101 64-65 0.8674207162269489 1101 66-67 0.8447081524361906 1101 68-69 1.1158409040868733 1101 70-71 1.4444523351743328 1101 72-73 1.5053801687451944 1101 74-75 1.7188508637145574 1101 76-77 1.9276087254876124 1101 78-79 2.0584246565299864 1101 80-81 2.3221612680990873 1101 82-83 2.2879191939713053 1101 84-85 2.253028975065252 1101 86-87 2.4660318694162484 1101 88-89 3.021835408799685 1101 90-91 3.278768515173411 1101 92-93 3.4166881367884834 1101 94-95 3.774068206590144 1101 96-97 4.2911886488382756 1101 98-99 4.507496668276385 1101 100-101 4.481071742926126 1102 1 0.48531366454479397 1102 2 0.37569076320143324 1102 3 0.24097704865405944 1102 4 0.2914080700257031 1102 5 0.40074428026601083 1102 6 0.39814258392968327 1102 7 0.26772299173805436 1102 8 0.36648278802045553 1102 9 0.5549296856341783 1102 10-11 0.5122848928140229 1102 12-13 0.4985348917274095 1102 14-15 0.651455601627859 1102 16-17 0.5715939426696863 1102 18-19 0.5694690703715253 1102 20-21 0.5931538982520337 1102 22-23 0.5920372910406613 1102 24-25 0.5317497067790242 1102 26-27 0.6695794374338604 1102 28-29 0.5142187792283579 1102 30-31 0.6133252524483765 1102 32-33 0.6578249733484256 1102 34-35 0.5985768795214668 1102 36-37 0.6959754434519425 1102 38-39 0.7887939547009424 1102 40-41 0.7841371684620384 1102 42-43 0.9387195666715371 1102 44-45 0.8673521082467346 1102 46-47 0.9273898206529978 1102 48-49 1.1630891043284848 1102 50-51 1.1698508671109664 1102 52-53 1.1763963169965521 1102 54-55 1.1230779296434221 1102 56-57 1.3526876469368645 1102 58-59 1.2127035689950105 1102 60-61 1.158494886577742 1102 62-63 1.26703012863792 1102 64-65 1.1770070565742792 1102 66-67 1.1204155359993315 1102 68-69 1.4706079086749746 1102 70-71 1.558927989009721 1102 72-73 1.7344057136587239 1102 74-75 1.559936918065958 1102 76-77 1.68742507958709 1102 78-79 1.9800562937654398 1102 80-81 2.075531152118611 1102 82-83 2.3321737181836006 1102 84-85 2.380196154901931 1102 86-87 2.568715971693262 1102 88-89 2.6362709015037495 1102 90-91 3.056579490115297 1102 92-93 3.4867912170255515 1102 94-95 3.733158617815789 1102 96-97 4.01349328927445 1102 98-99 4.463658967927106 1102 100-101 3.905719724525028 1103 1 0.513394335823218 1103 2 0.5119120878798142 1103 3 0.4051437519182244 1103 4 0.27728167123384395 1103 5 0.3869260088107822 1103 6 0.36196772627034335 1103 7 0.4842182168268465 1103 8 0.4012969270276727 1103 9 0.4575359470649971 1103 10-11 0.5479564758235327 1103 12-13 0.5792249411269523 1103 14-15 0.807957725935637 1103 16-17 0.8464222767845584 1103 18-19 0.7223774382599828 1103 20-21 0.6857029542490451 1103 22-23 0.7507944116154945 1103 24-25 0.7352100830202133 1103 26-27 0.7054645845628897 1103 28-29 0.8046305648510597 1103 30-31 0.809171341781223 1103 32-33 1.0095661407189453 1103 34-35 0.9590826758570898 1103 36-37 0.9771749300480437 1103 38-39 1.0480275038886973 1103 40-41 1.0234518790985874 1103 42-43 1.219655551269419 1103 44-45 1.1322930965492262 1103 46-47 1.2727201685748 1103 48-49 1.2532606214624877 1103 50-51 1.2407247587379828 1103 52-53 1.2927154490833033 1103 54-55 1.4066308715865574 1103 56-57 1.4208050384937465 1103 58-59 1.5832879625933245 1103 60-61 1.7661000359167858 1103 62-63 1.7845416781005028 1103 64-65 1.9292811779863648 1103 66-67 2.035168035372454 1103 68-69 2.093431715088844 1103 70-71 2.2521960796260743 1103 72-73 2.2577376822594886 1103 74-75 2.184196172865306 1103 76-77 2.365367553984189 1103 78-79 2.534350809665515 1103 80-81 2.7159302584784974 1103 82-83 2.7373158321779663 1103 84-85 2.854341792173013 1103 86-87 2.9205152228375866 1103 88-89 3.2739078410838722 1103 90-91 3.404351127383549 1103 92-93 3.7116508597111775 1103 94-95 3.87232379815174 1103 96-97 4.317386531298201 1103 98-99 4.820472194594075 1103 100-101 4.321030748966962 1104 1 0.321599728429387 1104 2 0.28913100019267546 1104 3 0.3331073938369542 1104 4 0.29489102554691016 1104 5 0.36665854267370435 1104 6 0.3658976071136095 1104 7 0.2742649374704982 1104 8 0.3264465094698821 1104 9 0.4357707469427865 1104 10-11 0.4267485498636532 1104 12-13 0.34432958518438994 1104 14-15 0.5625903259967373 1104 16-17 0.6288110129083933 1104 18-19 0.540804722097441 1104 20-21 0.45307361317561146 1104 22-23 0.4214663868675572 1104 24-25 0.517608048698996 1104 26-27 0.6386219198312446 1104 28-29 0.5901799461526167 1104 30-31 0.5934992392249541 1104 32-33 0.6580868658436998 1104 34-35 0.5931324136104266 1104 36-37 0.773258416146426 1104 38-39 0.757175265880754 1104 40-41 0.8038365287574152 1104 42-43 0.6657116669634817 1104 44-45 0.8830441913653999 1104 46-47 0.9532372146080021 1104 48-49 1.0349125844933482 1104 50-51 1.1558551818971665 1104 52-53 1.0111373224920044 1104 54-55 1.3177241601088099 1104 56-57 1.4377651413555483 1104 58-59 1.336528809420905 1104 60-61 1.5229499569881781 1104 62-63 1.5433648143669316 1104 64-65 1.5124092332873182 1104 66-67 1.4581951384379401 1104 68-69 1.6298063459066405 1104 70-71 1.9610424489101135 1104 72-73 1.909576909777563 1104 74-75 1.9698378497163134 1104 76-77 1.9773599284622385 1104 78-79 2.343480430808203 1104 80-81 2.2503819592626932 1104 82-83 2.611822846639722 1104 84-85 2.6733483101483664 1104 86-87 2.7419997171852764 1104 88-89 2.811954612649206 1104 90-91 3.223035247456874 1104 92-93 3.5748847684595226 1104 94-95 3.851692878506153 1104 96-97 4.151072179022005 1104 98-99 4.082802879999921 1104 100-101 3.9457726396890322 1105 1 0.3355683076643565 1105 2 0.2743085958210898 1105 3 0.28679591842711716 1105 4 0.19385505104781942 1105 5 0.12725735870284893 1105 6 0.2799003393540431 1105 7 0.3067672143375262 1105 8 0.24975024353180686 1105 9 0.2260029873799425 1105 10-11 0.1386964096086487 1105 12-13 0.17629679829914835 1105 14-15 0.36942092708963514 1105 16-17 0.4302226704675931 1105 18-19 0.46723914832695357 1105 20-21 0.4003641414087653 1105 22-23 0.28852581309706693 1105 24-25 0.3288159266589261 1105 26-27 0.2908020200133947 1105 28-29 0.3831615035296707 1105 30-31 0.5246752410464453 1105 32-33 0.4937881408892366 1105 34-35 0.4038963024993123 1105 36-37 0.5231559571300366 1105 38-39 0.5750885172468756 1105 40-41 0.7758253721089687 1105 42-43 0.7405978236119282 1105 44-45 0.6857536631322532 1105 46-47 0.9223344368302193 1105 48-49 0.998351792143616 1105 50-51 0.9577506737004384 1105 52-53 0.9334449272278889 1105 54-55 1.0351194242253854 1105 56-57 1.1379586750531772 1105 58-59 1.0729074524081526 1105 60-61 1.2361045562595834 1105 62-63 1.3300622187385116 1105 64-65 1.5848989873856745 1105 66-67 1.6409421785108016 1105 68-69 1.6128892238665706 1105 70-71 1.856693632880976 1105 72-73 1.8714336948413148 1105 74-75 1.7074346165651235 1105 76-77 1.9049612490451189 1105 78-79 1.9355227805349777 1105 80-81 2.0137585530696143 1105 82-83 2.159289832067774 1105 84-85 2.1009211698933576 1105 86-87 2.2633453456005803 1105 88-89 2.747143592612776 1105 90-91 3.0077517775115226 1105 92-93 3.3019453331225463 1105 94-95 3.5741655561017858 1105 96-97 3.6896821516996 1105 98-99 4.130935849034525 1105 100-101 3.5580278764832016 1106 1 0.13190453827278859 1106 2 0.03664218587500301 1106 3 -0.028357930547834087 1106 4 0.08225586521879791 1106 5 0.1544614882367199 1106 6 -0.018078902953504894 1106 7 0.08307832300367579 1106 8 0.07163200536846404 1106 9 0.08490385656246957 1106 10-11 0.12785546634389533 1106 12-13 0.1493351780255594 1106 14-15 0.2808509523949496 1106 16-17 0.1707386788293519 1106 18-19 0.17013917399900436 1106 20-21 0.16743789356338112 1106 22-23 0.14749407143131776 1106 24-25 0.036915002464844804 1106 26-27 0.18108789671416758 1106 28-29 0.29225628843450124 1106 30-31 0.10415593199154216 1106 32-33 0.11477963243579836 1106 34-35 0.1126770631257159 1106 36-37 0.2954759776676852 1106 38-39 0.0319305790798623 1106 40-41 0.3384165996000661 1106 42-43 0.2591307571946473 1106 44-45 0.37072129800223763 1106 46-47 0.49512526457071715 1106 48-49 0.6401069357237716 1106 50-51 0.5508146337987299 1106 52-53 0.5209504283831237 1106 54-55 0.2682648014212603 1106 56-57 0.4637765134659091 1106 58-59 0.465049969003303 1106 60-61 0.5195080293222532 1106 62-63 0.5918058098926551 1106 64-65 0.7543625330636026 1106 66-67 0.9913630168868615 1106 68-69 0.805691879090837 1106 70-71 0.9556080715797677 1106 72-73 1.2079246912838997 1106 74-75 1.2420157952793218 1106 76-77 1.3331601708186902 1106 78-79 1.5765481041862763 1106 80-81 1.6664380741023628 1106 82-83 1.839943185938754 1106 84-85 2.0515175868075772 1106 86-87 1.8689991579011611 1106 88-89 2.0907251197334737 1106 90-91 2.3583330662488216 1106 92-93 2.5130232844923377 1106 94-95 2.6507420955083916 1106 96-97 2.905721228239006 1106 98-99 3.429987816614389 1106 100-101 3.8095053950954423 1107 1 0.25035831949467635 1107 2 0.12529938507239535 1107 3 0.09015206737647219 1107 4 0.06447149519181039 1107 5 0.1472069390081856 1107 6 0.14218626690742298 1107 7 0.16154300161711888 1107 8 0.27812892642291587 1107 9 0.22112298061976077 1107 10-11 0.1810619805623972 1107 12-13 0.19334161267580185 1107 14-15 0.4703008070963932 1107 16-17 0.4300853542600578 1107 18-19 0.3365951001043186 1107 20-21 0.3449693749167366 1107 22-23 0.3568723147026063 1107 24-25 0.30326454927173785 1107 26-27 0.32528071135472914 1107 28-29 0.4111528842969463 1107 30-31 0.5017645599580547 1107 32-33 0.4075570834840221 1107 34-35 0.38886193823815063 1107 36-37 0.31715875978904506 1107 38-39 0.40697122808006014 1107 40-41 0.4080855264664649 1107 42-43 0.361237153790519 1107 44-45 0.21140186605039446 1107 46-47 0.47366605195049516 1107 48-49 0.5005805398198007 1107 50-51 0.5380824155741379 1107 52-53 0.48592612547023606 1107 54-55 0.614917745446725 1107 56-57 0.7931488756052616 1107 58-59 0.5554542103372881 1107 60-61 0.6709990692447647 1107 62-63 0.9181822542294711 1107 64-65 1.0646536490948755 1107 66-67 1.101791215184793 1107 68-69 1.2916978121151246 1107 70-71 1.4326418188986594 1107 72-73 1.6372866749551136 1107 74-75 1.6433308336796557 1107 76-77 1.7241633362514754 1107 78-79 1.6312811180934226 1107 80-81 1.855658305196254 1107 82-83 2.026961019606503 1107 84-85 2.29620770304642 1107 86-87 2.3108356278381983 1107 88-89 2.5233234557190833 1107 90-91 2.5183638557042975 1107 92-93 2.932559453453795 1107 94-95 3.4639781018738525 1107 96-97 3.504562299296918 1107 98-99 3.6100866715234012 1107 100-101 3.3420963796661205 1108 1 0.21881538690449887 1108 2 0.08093156730042494 1108 3 0.09411873599196241 1108 4 0.03245272476976169 1108 5 0.09935519253717473 1108 6 0.10887964870151023 1108 7 0.0691281489806812 1108 8 0.01588544036675188 1108 9 0.1597861848759905 1108 10-11 0.08339565656392978 1108 12-13 0.030490580773552267 1108 14-15 0.23093468642521486 1108 16-17 0.3057223760674006 1108 18-19 0.11450510016927495 1108 20-21 0.13193572197628356 1108 22-23 0.05224576619962562 1108 24-25 0.042167621267786615 1108 26-27 0.15266505677726627 1108 28-29 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-1.4294552023838918 2316 60-61 -1.578535150106866 2316 62-63 -1.6842764324384731 2316 64-65 -1.706780023740798 2316 66-67 -1.7252640772125396 2316 68-69 -1.7804696063873777 2316 70-71 -1.803651174066637 2316 72-73 -1.6962528486500865 2316 74-75 -1.973300993535485 2316 76-77 -2.156123626971617 2316 78-79 -3.0254840635327973 2316 80-81 -2.85854998061804 2316 82-83 -2.527398052106779 2316 84-85 -2.7340966210917443 2316 86-87 -2.7335045727015412 2316 88-89 -3.1050763901498684 2316 90-91 -3.1878936955880306 2316 92-93 -3.2385545950551418 2316 94-95 -3.4095977385073546 2316 96-97 -3.7784036789553603 2316 98-99 -3.7976248916929123 2316 100-101 -3.545056966614972 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4114.0 3 1085.0 4 1046.0 5 1161.0 6 1426.0 7 1754.0 8 2211.0 9 2924.0 10 3653.0 11 4982.0 12 6765.0 13 9400.0 14 11125.0 15 15268.0 16 20230.0 17 27649.0 18 25318.0 19 15900.0 20 14377.0 21 14840.0 22 15714.0 23 17192.0 24 19289.0 25 21560.0 26 24565.0 27 28255.0 28 33307.0 29 38816.0 30 46301.0 31 55167.0 32 68067.0 33 87170.0 34 118860.0 35 175087.0 36 193431.0 37 218863.0 38 120813.0 39 7040.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.75486770219385 19.53042232967045 15.491893578564191 19.222816389571513 2 15.228850966205368 21.686590886119568 41.42231996467162 21.662238183003442 3 18.88232771955343 28.741200766354225 30.555186188256112 21.821285325836232 4 11.702163747927303 18.442606267351643 38.09124486744228 31.763985117278775 5 11.906224190852473 39.54723163067327 33.053285349667505 15.493258828806757 6 27.670311414176513 38.15984136118832 18.2625331176164 15.907314107018763 7 25.61799018108084 36.96605302379158 20.38901209082466 17.026944704302917 8 26.474905743059445 34.03886411398476 19.561350787769594 19.9248793551862 9 28.066444290223647 16.866825817104534 20.60061991334238 34.46610997932944 10-11 24.191140579721452 28.77505324668232 25.730859263565904 21.30294691003032 12-13 26.761963134831625 26.963340041374355 27.027582564701273 19.247114259092744 14-15 23.429361589986172 26.78059953133951 28.20286100644993 21.587177872224387 16-17 19.47658376976225 30.633765675234475 28.511467896909505 21.378182658093767 18-19 19.369671593432276 27.271028189547625 34.08806443712828 19.27123577989182 20-21 21.368677939055285 25.837253702017605 34.2016152778362 18.59245308109091 22-23 20.446262848831527 26.55552947497531 33.94303096744782 19.055176708745346 24-25 19.642748093674893 26.699916812437152 34.55232063445105 19.1050144594369 26-27 19.52961003469645 26.845241891383964 34.44847298982446 19.176675084095134 28-29 19.233268818911206 27.083880281312727 34.573319521412884 19.10953137836318 30-31 19.39734123527544 27.133822862988684 34.42395466152172 19.044881240214156 32-33 19.11419578989285 27.235379254600534 34.11125669944722 19.539168256059394 34-35 19.591757401754585 27.258449735096168 34.01091093742951 19.13888192571974 36-37 19.725918762679445 27.008520858822248 33.95664507253126 19.30891530596704 38-39 19.617111296953073 27.144558594817724 33.91890507884379 19.31942502938541 40-41 19.662942686149286 27.15045631873945 33.94589557300286 19.240705422108398 42-43 19.38159971405337 27.32733496518247 33.64145846347093 19.649606857293232 44-45 19.45118671953786 27.641643727107066 33.257783682496154 19.649385870858925 46-47 19.71800989254532 27.84600593145758 32.47576462050033 19.96021955549677 48-49 19.716396863971827 28.032948779427397 32.094911042093834 20.155743314506946 50-51 20.154986449790982 28.355575660081 31.6540139272819 19.835423962846114 52-53 20.04542507507548 28.634412919222306 31.4201405749412 19.900021430761008 54-55 19.630697530263454 28.56702517006503 31.519227241330082 20.283050058341434 56-57 19.946993117446933 28.867116913203606 30.96226548811804 20.22362448123142 58-59 19.893783355888488 28.439672615101657 31.256224426233786 20.410319602776063 60-61 19.892363184560413 28.380791938666476 31.52498600368736 20.201858873085744 62-63 20.127540501535425 28.252635448202522 31.52188656314767 20.097937487114383 64-65 19.83005195965167 28.354920738048822 31.571244539285775 20.243782763013733 66-67 19.729446282412482 28.56269015679109 31.312883344053716 20.394980216742713 68-69 19.85563141251045 28.60967245122101 31.221945001861574 20.312751134406966 70-71 19.810184444185218 28.68074526410595 30.921596979010964 20.587473312697867 72-73 19.837429967928177 28.624424636640278 30.881185050149103 20.656960345282442 74-75 19.793908538903164 28.872166530635496 30.691039473679748 20.642885456781592 76-77 19.902305908776803 28.628179923681152 30.85104940109304 20.618464766449005 78-79 19.95776990941588 28.677737682438124 30.72908789921745 20.63540450892854 80-81 19.854057716344737 29.030605220663187 30.447504793192483 20.6678322697996 82-83 19.984612092833366 28.970668370255154 30.23296504761333 20.81175448929815 84-85 20.051351294531415 28.649200535214497 30.26220220555573 21.037245964698357 86-87 20.10747412744659 28.62894708379205 30.259842725254728 21.003736063506636 88-89 20.266614937164935 28.38433708462657 30.280546632517964 21.068501345690535 90-91 20.061108188761832 28.609403769525333 30.35657296269579 20.972915079017046 92-93 20.248267798533263 28.508952038882423 30.22051934338264 21.022260819201673 94-95 20.043655740974128 28.46274949771479 30.128905174249898 21.364689587061186 96-97 20.28777998250761 28.171224646972863 29.936334893450052 21.604660477069473 98-99 20.307426958374226 28.056753972296512 29.801430955935267 21.834388113394 100-101 21.091825990466205 27.34719295046727 30.011683801890797 21.549297257175727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10453.0 1 6389.0 2 3594.5 3 4359.0 4 2923.5 5 2426.5 6 4612.5 7 10009.0 8 15296.5 9 15911.0 10 11380.5 11 6861.5 12 5858.5 13 5882.5 14 5814.0 15 5585.0 16 5377.0 17 5374.0 18 5509.5 19 5611.5 20 5814.0 21 6365.5 22 7122.0 23 8016.0 24 9051.0 25 10535.0 26 12582.0 27 14729.0 28 16305.0 29 18253.5 30 20742.0 31 22702.5 32 25338.0 33 28246.5 34 30701.0 35 32978.0 36 35998.0 37 38750.0 38 40217.0 39 41128.0 40 41719.0 41 42544.0 42 43367.0 43 44968.5 44 46950.0 45 48371.0 46 48295.0 47 48920.0 48 54152.5 49 54563.5 50 49693.5 51 45778.5 52 41780.5 53 39297.5 54 37255.0 55 34391.5 56 30959.0 57 27787.5 58 25181.5 59 22418.5 60 19047.0 61 16070.5 62 13454.5 63 9987.0 64 6873.0 65 5274.0 66 3952.0 67 2862.5 68 2152.0 69 1622.5 70 1215.5 71 930.5 72 752.5 73 704.0 74 570.5 75 425.0 76 315.5 77 199.0 78 131.5 79 75.0 80 36.0 81 24.5 82 19.0 83 12.0 84 10.0 85 9.5 86 8.0 87 8.0 88 5.5 89 3.0 90 2.5 91 0.5 92 1.5 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47046059434809884 2 0.03770194443031752 3 0.049704182135652406 4 0.054315211310583325 5 0.0792012070046958 6 0.08062520130871857 7 0.04285544762582855 8 0.038176609198325114 9 0.04394039566698876 10-11 0.04675447964874807 12-13 0.04227906897896218 14-15 0.035498143721710826 16-17 0.03692213802573361 18-19 0.045296580718439027 20-21 0.0541795928054383 22-23 0.04482191595043143 24-25 0.0642153621861703 26-27 0.0541795928054383 28-29 0.043126684636118594 30-31 0.044550678940141385 32-33 0.042685924494397266 34-35 0.047432572174473206 36-37 0.043160589262404854 38-39 0.024004475410669787 40-41 0.021495533065486783 42-43 0.02197019783349438 44-45 0.013426232009357676 46-47 0.02593703910898642 48-49 0.025496278967265085 50-51 0.014172133787655326 52-53 0.014172133787655326 54-55 0.014612893929376664 56-57 0.013527945888216448 58-59 0.01298547186763634 60-61 0.015562223465391854 62-63 0.01444337079794538 64-65 0.014612893929376664 66-67 0.008103205682415366 68-69 0.011256335927037245 70-71 0.01634202986997576 72-73 0.015053654071098 74-75 0.012578616352201257 76-77 0.00827272881384665 78-79 0.012476902473342488 80-81 0.01613860211225822 82-83 0.012917662615063826 84-85 0.01193442845276238 86-87 0.0030175117394768518 88-89 0.02637779925070776 90-91 0.030819305294207396 92-93 0.01549441421281934 94-95 0.03139568394107376 96-97 0.025055518825543747 98-99 0.022105816338639405 100-101 0.14738341046635814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1474725.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.84589303662989 #Duplication Level Percentage of deduplicated Percentage of total 1 74.92846278666823 39.59661529823373 2 13.950886172962814 14.744940769251839 3 4.881968103571358 7.739758926287125 4 2.205376850029189 4.661804364884091 5 1.1825089929947357 3.1245371879326362 6 0.6984800743915425 2.2147081979707646 7 0.44914034867813607 1.6614655977275603 8 0.3013639563208154 1.2740677920660333 9 0.21154728200706557 1.006146453342165 >10 1.0803615706982648 10.256255000582142 >50 0.07013054447164209 2.5300496859456696 >100 0.03501087857248863 3.418392905530322 >500 0.0030891493839950352 1.1162140660332835 >1k 0.001158431018998138 1.8409709748440477 >5k 1.287145576664598E-4 0.6451730905743872 >10k+ 3.861436729993794E-4 4.168899688794267 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26273 1.7815524928376476 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20569 1.3947685161640306 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14447 0.9796402719151028 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9485 0.6431707606502907 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4581 0.31063418603468446 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4178 0.2833070572479615 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4142 0.280865924155351 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3578 0.24262150570445337 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2652 0.17983013782230586 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2380 0.16138602112258219 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2194 0.14877350014409466 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1907 0.12931224465578328 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.780925257251352E-5 0.0 16-17 0.0 0.0 0.0 2.0342775771754057E-4 0.0 18-19 0.0 0.0 0.0 3.729508891488244E-4 0.0 20-21 0.0 0.0 0.0 7.798064045839055E-4 0.0 22-23 0.0 0.0 0.0 0.0016274220617403246 0.0 24-25 0.0 0.0 0.0 0.003865127396633271 0.0 26-27 0.0 0.0 0.0 0.007391208530403974 0.0 28-29 0.0 0.0 0.0 0.016036888233399448 0.0 30-31 0.0 0.0 0.0 0.03624404550000848 0.0 32-33 0.0 0.0 0.0 0.06580887962162438 0.0 34-35 0.0 0.0 0.0 0.10717252369085761 0.0 36-37 0.0 0.0 0.0 0.17223550153418432 0.0 38-39 0.0 0.0 0.0 0.2675414060248521 0.0 40-41 0.0 0.0 0.0 0.3702046144196376 0.0 42-43 0.0 0.0 0.0 0.47025716659038125 0.0 44-45 0.0 0.0 0.0 0.5810235806675821 0.0 46-47 0.0 0.0 0.0 0.716370848802319 0.0 48-49 0.0 0.0 0.0 0.8644662564206886 0.0 50-51 0.0 0.0 0.0 1.018122022750004 0.0 52-53 0.0 0.0 0.0 1.168353421824408 0.0 54-55 0.0 0.0 0.0 1.3218735696485786 0.0 56-57 0.0 0.0 0.0 1.4906168946752785 0.0 58-59 0.0 0.0 0.0 1.6859075420841174 0.0 60-61 0.0 0.0 0.0 1.8952347047754667 0.0 62-63 0.0 0.0 0.0 2.1040872026988082 0.0 64-65 0.0 0.0 0.0 2.3082608621946465 0.0 66-67 0.0 0.0 0.0 2.5228771465866515 0.0 68-69 0.0 0.0 0.0 2.7656342707962502 0.0 70-71 0.0 0.0 0.0 3.0358541422977163 0.0 72-73 0.0 0.0 0.0 3.3073284849717743 0.0 74-75 0.0 0.0 0.0 3.562901557917578 0.0 76-77 0.0 0.0 0.0 3.831934767499025 0.0 78-79 0.0 0.0 0.0 4.125955686653445 0.0 80-81 0.0 0.0 0.0 4.4455067894014135 0.0 82-83 0.0 0.0 0.0 4.773534048720949 0.0 84-85 0.0 0.0 0.0 5.098645510179864 0.0 86-87 0.0 6.780925257251352E-5 0.0 5.426130295478818 0.0 88-89 0.0 6.780925257251352E-5 0.0 5.76758378680771 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10790 0.0 41.54203 1 GTATCAA 17345 0.0 35.199768 1 TATCAAC 21520 0.0 28.245605 2 ATCAACG 21790 0.0 27.78656 3 TCAACGC 22045 0.0 27.486708 4 CAACGCA 22325 0.0 27.184544 5 AACGCAG 22855 0.0 26.61472 6 GTACATG 13840 0.0 24.66116 1 GAGTACT 14225 0.0 24.16605 12-13 ACGCAGA 25370 0.0 23.988543 7 TACATGG 14015 0.0 23.872734 2 CGCAGAG 25425 0.0 23.861908 8 GTGTAAG 590 0.0 23.463326 1 GCAGAGT 25865 0.0 22.868206 9 GTACTTT 15055 0.0 22.612059 14-15 ACATGGG 14145 0.0 22.477385 3 AGTACTT 14450 0.0 21.948399 12-13 CATTCCG 175 4.0439336E-6 21.718365 9 GTATAGC 695 0.0 21.292196 1 ACTTTTT 16665 0.0 20.766817 16-17 >>END_MODULE