FastQCFastQC Report
Thu 26 May 2016
SRR938194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474725
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253961.7220837783315532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210011.4240621132753564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146750.995100781501636No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92980.6304904304192307No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47510.3221617589720117No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42050.2851379070674193No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39240.26608350709454304No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33310.22587262031904254No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27590.1870857278475648No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23930.16226754140602487No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23130.15684280120022376No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20080.13616097916560713No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14800.10035769380732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120150.039.541091
GTATCAA186200.032.6079561
CAACGCG700.00839461927.1413385
AACGCGG700.00839461927.1413386
TATCAAC230200.026.2515012
ATCAACG231600.026.0901583
TCAACGC235300.025.7369774
CAACGCA237800.025.3865095
AACGCAG242700.024.8543976
GAGTACT148750.023.94823612-13
GTACATG144950.022.8775541
AGTACTT150050.022.74364512-13
GTACTTT155300.022.72409414-15
TACATGG145450.022.3416232
ACGCAGA269400.022.2853157
ATGGGCG2354.654794E-922.2327985
CGCAGAG270050.022.1964998
GCAGAGT278050.021.455379
ACATGGG147950.020.8380893
ACTTTTT173200.020.47157316-17
Produced by FastQC (version 0.11.5)