##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938194_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1474725 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50981640644866 33.0 31.0 34.0 31.0 34.0 2 32.70753869365475 34.0 31.0 34.0 31.0 34.0 3 32.640537049280375 34.0 31.0 34.0 31.0 34.0 4 36.18304158402414 37.0 35.0 37.0 35.0 37.0 5 36.1766220820831 37.0 35.0 37.0 35.0 37.0 6 36.26089406499517 37.0 37.0 37.0 35.0 37.0 7 36.23065588499551 37.0 37.0 37.0 35.0 37.0 8 36.23752699655868 37.0 37.0 37.0 35.0 37.0 9 37.91432639983726 39.0 38.0 39.0 35.0 39.0 10-11 37.89975453050569 39.0 38.0 39.0 35.0 39.0 12-13 37.838188814863784 39.0 38.0 39.0 35.0 39.0 14-15 39.00410585024326 40.0 38.0 41.0 35.5 41.0 16-17 38.958962179389374 40.0 38.0 41.0 35.5 41.0 18-19 39.04919493464883 40.0 38.5 41.0 36.0 41.0 20-21 39.111221075115694 40.0 39.0 41.0 35.5 41.0 22-23 39.09234196206072 40.0 39.0 41.0 35.0 41.0 24-25 39.025094509145774 40.0 38.5 41.0 35.0 41.0 26-27 38.83158215938565 40.0 38.0 41.0 35.0 41.0 28-29 38.71546661241926 40.0 38.0 41.0 35.0 41.0 30-31 38.3960633338419 40.0 37.5 41.0 34.5 41.0 32-33 38.06253538795369 40.0 36.5 41.0 34.0 41.0 34-35 37.80853888013019 40.0 36.0 41.0 33.5 41.0 36-37 37.66826052314838 40.0 36.0 41.0 33.0 41.0 38-39 37.563500652664054 40.0 36.0 41.0 33.0 41.0 40-41 37.38292698638729 40.0 35.0 41.0 33.0 41.0 42-43 37.1168685687162 40.0 35.0 41.0 33.0 41.0 44-45 36.71637356117242 40.0 35.0 41.0 31.5 41.0 46-47 36.139007272542344 40.0 35.0 41.0 30.5 41.0 48-49 35.59751614707827 39.0 35.0 41.0 27.5 41.0 50-51 35.244871077658544 39.0 35.0 41.0 26.5 41.0 52-53 34.793070911525874 38.5 35.0 40.0 24.0 41.0 54-55 34.51636745833969 38.0 35.0 40.0 23.0 41.0 56-57 34.281267693976844 38.0 34.0 40.0 23.0 41.0 58-59 33.94936784824289 38.0 34.0 40.0 22.0 41.0 60-61 33.58979674176541 37.0 34.0 40.0 20.5 41.0 62-63 33.18880367526149 37.0 33.0 40.0 18.5 41.0 64-65 32.79359033040058 36.0 33.0 39.0 16.5 41.0 66-67 32.324676465103664 35.5 32.0 39.0 11.0 40.0 68-69 31.83903100578074 35.0 31.5 38.5 7.5 40.0 70-71 31.307152180915086 35.0 31.0 38.0 6.5 40.0 72-73 30.875438132533183 35.0 31.0 37.0 2.0 39.0 74-75 30.350973571343808 35.0 30.0 36.5 2.0 39.0 76-77 29.10016002983607 33.5 28.5 35.0 2.0 37.5 78-79 29.80087033175677 34.0 30.0 36.0 2.0 38.0 80-81 29.644143823424706 34.0 30.0 35.0 2.0 37.0 82-83 29.23729373272983 34.0 29.5 35.0 2.0 37.0 84-85 28.771453660852025 34.0 29.0 35.0 2.0 36.0 86-87 28.275599857600568 34.0 29.0 35.0 2.0 36.0 88-89 27.716067741443318 33.5 27.0 35.0 2.0 35.5 90-91 27.22907355608673 33.0 26.0 35.0 2.0 35.0 92-93 26.671687602773396 33.0 25.0 35.0 2.0 35.0 94-95 26.007149807591247 32.5 24.0 35.0 2.0 35.0 96-97 25.289372255844313 32.0 19.5 34.5 2.0 35.0 98-99 24.405377951821528 32.0 9.0 34.0 2.0 35.0 100-101 21.13091559443286 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07288067404635257 1101 2 0.0214389891915161 1101 3 0.18066264453863567 1101 4 0.0478584929087944 1101 5 0.010932899644870986 1101 6 0.03858826919267244 1101 7 0.11839212413094202 1101 8 0.13107717141151198 1101 9 0.09353663450401939 1101 10-11 0.11765720664372736 1101 12-13 0.16254163872989125 1101 14-15 0.2548099916662636 1101 16-17 0.20267894147736598 1101 18-19 0.23681976680294525 1101 20-21 0.20326630615901564 1101 22-23 0.10324077606388471 1101 24-25 0.1723362540622162 1101 26-27 0.2651203897555092 1101 28-29 0.11070043436369303 1101 30-31 0.04969482613897469 1101 32-33 0.1110710618719466 1101 34-35 0.010284351313060824 1101 36-37 0.1170058237394187 1101 38-39 0.2205734583793273 1101 40-41 0.10124947401137518 1101 42-43 0.22191724894420872 1101 44-45 0.32930807222832925 1101 46-47 0.3759100164205975 1101 48-49 0.596232514399901 1101 50-51 0.6834403246592586 1101 52-53 0.4883011419095453 1101 54-55 0.5826979163809938 1101 56-57 0.6306144823238427 1101 58-59 0.6484005286194687 1101 60-61 0.5428737544797997 1101 62-63 0.579375332427702 1101 64-65 0.5479576062345473 1101 66-67 0.5301485294880806 1101 68-69 0.6803287408976466 1101 70-71 0.722317287709263 1101 72-73 0.6796068299774056 1101 74-75 0.5767106883398654 1101 76-77 0.889693325421117 1101 78-79 0.8141193567925136 1101 80-81 1.003098335693572 1101 82-83 1.0482631639874818 1101 84-85 1.191080758934664 1101 86-87 1.4601989776888438 1101 88-89 1.648210986362841 1101 90-91 1.7799672384714853 1101 92-93 1.9545905711501064 1101 94-95 2.072662993686844 1101 96-97 2.266438473391883 1101 98-99 2.4041102159889896 1101 100-101 3.163951854900464 1102 1 0.16225988031636263 1102 2 0.10259003155542246 1102 3 0.3275136467414157 1102 4 0.1515959121301549 1102 5 0.0929893929696064 1102 6 0.04987963211542734 1102 7 0.15738614864140033 1102 8 0.08544195618436135 1102 9 0.18746743565773727 1102 10-11 0.21446013718931312 1102 12-13 0.14104143792422974 1102 14-15 0.3648746145775732 1102 16-17 0.3034073183591559 1102 18-19 0.3750762869017663 1102 20-21 0.40084507674159653 1102 22-23 0.42314365914631935 1102 24-25 0.23879075636843794 1102 26-27 0.2367203848874908 1102 28-29 0.24624364408998645 1102 30-31 0.21145838660581262 1102 32-33 0.24599907608655514 1102 34-35 0.1667807185561827 1102 36-37 0.30780527365362786 1102 38-39 0.3134898867160487 1102 40-41 0.35670286884397484 1102 42-43 0.37957950599979284 1102 44-45 0.4036694493900228 1102 46-47 0.36479755308284467 1102 48-49 0.3721224485599919 1102 50-51 0.49500307946946265 1102 52-53 0.3417452631718234 1102 54-55 0.5440184873992564 1102 56-57 0.6457594883480127 1102 58-59 0.4003148758793813 1102 60-61 0.5338222873815042 1102 62-63 0.4944546202369793 1102 64-65 0.46482102187791696 1102 66-67 0.4308044946330867 1102 68-69 0.4927573940393408 1102 70-71 0.650445900689828 1102 72-73 0.5691460722707227 1102 74-75 0.6287309149460043 1102 76-77 0.6954515797506104 1102 78-79 0.7481510475761404 1102 80-81 0.8545344003895075 1102 82-83 0.9067961873904693 1102 84-85 1.0511399053280748 1102 86-87 1.038762669592117 1102 88-89 1.311005957702399 1102 90-91 1.3763916807794132 1102 92-93 1.5026969129578447 1102 94-95 1.3381822284347393 1102 96-97 1.4141870160296186 1102 98-99 1.918696617186523 1102 100-101 2.3187578036159167 1103 1 0.2946745927945926 1103 2 0.3433267321420672 1103 3 0.4363718472494327 1103 4 0.18808598051317205 1103 5 0.05579169422038888 1103 6 0.1138489922164041 1103 7 0.18595132359397581 1103 8 0.22320604256201193 1103 9 0.39911195729536075 1103 10-11 0.34634359563887074 1103 12-13 0.3306406882185513 1103 14-15 0.3827190832314642 1103 16-17 0.4222095317759411 1103 18-19 0.46223205199964923 1103 20-21 0.3544318914416067 1103 22-23 0.38842160417073046 1103 24-25 0.4838398229905252 1103 26-27 0.30774869435217056 1103 28-29 0.3563148746301579 1103 30-31 0.3474665013340186 1103 32-33 0.5114745059418979 1103 34-35 0.3509073440164272 1103 36-37 0.11241854197091072 1103 38-39 0.2670384511259911 1103 40-41 0.322218461503887 1103 42-43 0.29779648997858743 1103 44-45 0.418445394774281 1103 46-47 0.5223710396817793 1103 48-49 0.5382856044639226 1103 50-51 0.7007014518487935 1103 52-53 0.7397009268356101 1103 54-55 0.7504591987413178 1103 56-57 0.7184743522690624 1103 58-59 0.9181678445977326 1103 60-61 0.947970573839342 1103 62-63 0.915891711968257 1103 64-65 0.9065771635286595 1103 66-67 0.9103296101985379 1103 68-69 0.9673322845655967 1103 70-71 0.9349388080943761 1103 72-73 0.8172895165251752 1103 74-75 0.9286532101900775 1103 76-77 0.7497963876987406 1103 78-79 1.2264503830127715 1103 80-81 1.449392640564497 1103 82-83 1.3994482358351696 1103 84-85 1.5688664355875943 1103 86-87 1.622725952147011 1103 88-89 1.8436529344419732 1103 90-91 1.8832590245234329 1103 92-93 2.075802779844004 1103 94-95 2.0037408172540374 1103 96-97 2.3977818916240956 1103 98-99 2.7976677410508763 1103 100-101 2.940816250059136 1104 1 0.10024287877076432 1104 2 0.09822157886780047 1104 3 0.1581014021939282 1104 4 0.07158990148271727 1104 5 0.15111772029229087 1104 6 0.05918647566686275 1104 7 0.0041532789865854625 1104 8 0.06780070596969523 1104 9 3.3344115318811873E-4 1104 10-11 -0.020343121440262735 1104 12-13 0.025197478624903624 1104 14-15 0.10787757696809308 1104 16-17 0.16590892835400695 1104 18-19 0.07587269768658444 1104 20-21 0.0074439089586633145 1104 22-23 0.1089767778136661 1104 24-25 0.084160947850485 1104 26-27 -0.011252094933908552 1104 28-29 -0.012858730115745232 1104 30-31 0.08012735019846673 1104 32-33 0.17795371716503183 1104 34-35 0.12096348517133038 1104 36-37 0.2504567205023278 1104 38-39 0.2653869973119569 1104 40-41 0.2577783454024498 1104 42-43 0.22462394185760104 1104 44-45 0.23758399086349868 1104 46-47 0.49342970145996645 1104 48-49 0.44178424230891267 1104 50-51 0.32908774373187555 1104 52-53 0.32989945774506424 1104 54-55 0.4392459260047872 1104 56-57 0.5628077237936608 1104 58-59 0.5023101961610266 1104 60-61 0.5343627089854834 1104 62-63 0.49691415569979824 1104 64-65 0.5783573218950906 1104 66-67 0.5436409835563225 1104 68-69 0.7441902894540782 1104 70-71 0.8038488931335799 1104 72-73 0.6513615325145885 1104 74-75 0.6328567950765205 1104 76-77 0.6400897602417679 1104 78-79 0.7406613515431708 1104 80-81 0.8562206016603291 1104 82-83 0.8166686757985069 1104 84-85 1.0257975130903922 1104 86-87 1.0551191439180663 1104 88-89 1.1727954133094727 1104 90-91 1.1791989751294025 1104 92-93 1.2945047225061863 1104 94-95 1.459755622733212 1104 96-97 1.7474779528319537 1104 98-99 1.8398659027781399 1104 100-101 2.4421698120308264 1105 1 0.1057073596450806 1105 2 0.09628624189148383 1105 3 0.2049365570208863 1105 4 0.029012488003665737 1105 5 0.007549869654766894 1105 6 0.044238843608574996 1105 7 0.09786912598111996 1105 8 0.014840778829437795 1105 9 0.12723462512404637 1105 10-11 0.026497725554278873 1105 12-13 -0.009655663451596297 1105 14-15 0.08701009062929899 1105 16-17 0.05745605950153987 1105 18-19 0.09927889076490715 1105 20-21 0.06199764302059663 1105 22-23 0.1337832441160316 1105 24-25 0.09960379848801182 1105 26-27 0.07507531854514582 1105 28-29 0.1024816615053794 1105 30-31 0.024862095189362776 1105 32-33 0.09154092117231727 1105 34-35 0.1543309715101273 1105 36-37 0.2567351813402112 1105 38-39 0.34336400095494213 1105 40-41 0.42123462955544966 1105 42-43 0.411065198688199 1105 44-45 0.42537429140994476 1105 46-47 0.468361395449044 1105 48-49 0.4607106995038137 1105 50-51 0.33159229746593866 1105 52-53 0.40924258160663385 1105 54-55 0.5549506163508724 1105 56-57 0.4902155106789081 1105 58-59 0.5811922544488297 1105 60-61 0.47151548676326627 1105 62-63 0.5000505400349482 1105 64-65 0.46168496306083995 1105 66-67 0.4737127048677934 1105 68-69 0.43835012551964425 1105 70-71 0.6269647856654608 1105 72-73 0.563753381330617 1105 74-75 0.5802383979909109 1105 76-77 0.6531646691670865 1105 78-79 0.7918225537289629 1105 80-81 0.8690507473798164 1105 82-83 0.9420102058531512 1105 84-85 1.0373298446022332 1105 86-87 1.172867322424441 1105 88-89 1.2097261965517347 1105 90-91 1.0287151408853248 1105 92-93 1.192449850010739 1105 94-95 1.1740941928607178 1105 96-97 1.4241457579321377 1105 98-99 1.815218817167942 1105 100-101 2.136938710027181 1106 1 0.036987845213715786 1106 2 0.07384611279770326 1106 3 0.13428522024016587 1106 4 0.08555336158711668 1106 5 0.08934012894255261 1106 6 0.060915595726520166 1106 7 0.09537251836018612 1106 8 -0.002464021845369757 1106 9 -0.06361603684830186 1106 10-11 0.0490290821391568 1106 12-13 0.016269905917965843 1106 14-15 -0.050413810056511466 1106 16-17 0.04220227287301981 1106 18-19 0.00927128083348805 1106 20-21 0.06111654132257627 1106 22-23 0.09334012606124986 1106 24-25 0.07139295754475228 1106 26-27 0.01531856129278708 1106 28-29 0.007804487632199653 1106 30-31 0.07589310336774702 1106 32-33 0.1249219311844243 1106 34-35 -0.05735027321792785 1106 36-37 -0.03878871201072798 1106 38-39 0.1882873515252257 1106 40-41 0.2945512760512159 1106 42-43 0.3875014586361232 1106 44-45 0.4781111161431397 1106 46-47 -0.04432197639238211 1106 48-49 0.03158895893829339 1106 50-51 0.008375588623749763 1106 52-53 -0.12645074732577655 1106 54-55 -0.16051404789529045 1106 56-57 -0.17762152676562692 1106 58-59 -0.13792563530802937 1106 60-61 -0.07487713068640289 1106 62-63 -0.39582680254032 1106 64-65 -0.28261629853742676 1106 66-67 -0.08453994485531524 1106 68-69 -0.3022163100984976 1106 70-71 -0.23619351924523713 1106 72-73 -0.1825846829962252 1106 74-75 0.002301239520967613 1106 76-77 0.024180457482632534 1106 78-79 0.11966023297493678 1106 80-81 0.2958505420033575 1106 82-83 0.3152425013018636 1106 84-85 0.3064509433663076 1106 86-87 0.38171776435057936 1106 88-89 0.527083258201344 1106 90-91 0.5973640571577405 1106 92-93 0.5996944130356425 1106 94-95 0.9689162304886203 1106 96-97 1.0231234599162207 1106 98-99 1.391762808072695 1106 100-101 1.7462828806363433 1107 1 0.08800061072953014 1107 2 0.08755433946345192 1107 3 0.22724515935315281 1107 4 0.036438125995893245 1107 5 0.040815601162854875 1107 6 0.03805961426937898 1107 7 0.008620632938473705 1107 8 0.050303569658126435 1107 9 0.08365961526544652 1107 10-11 0.023482050381048225 1107 12-13 0.01352436865926876 1107 14-15 0.03556228487187241 1107 16-17 0.1335098733711888 1107 18-19 0.14153707340251032 1107 20-21 0.04183680701135728 1107 22-23 -0.08927637224360296 1107 24-25 0.06773411508989824 1107 26-27 0.14862047093093622 1107 28-29 0.1047350556803579 1107 30-31 -0.11450681360450687 1107 32-33 0.04487453617992543 1107 34-35 0.08926245424349588 1107 36-37 0.0077985017165289605 1107 38-39 -0.05769791105002042 1107 40-41 0.08653693646774485 1107 42-43 0.05329662226996845 1107 44-45 0.012379953062804816 1107 46-47 -0.003413082045192084 1107 48-49 0.056779230854161256 1107 50-51 0.02857943903542548 1107 52-53 -0.048383045118626455 1107 54-55 -0.05742861580506542 1107 56-57 0.023106263312563158 1107 58-59 -0.09718149914252905 1107 60-61 0.06236600222717925 1107 62-63 0.1576279300411514 1107 64-65 -0.024159144313394165 1107 66-67 0.11553746121954589 1107 68-69 0.15677620010699655 1107 70-71 0.35004376713129304 1107 72-73 0.21415864018927167 1107 74-75 0.2579713426137502 1107 76-77 0.3521781050298536 1107 78-79 0.6919285336737531 1107 80-81 0.6616258135732735 1107 82-83 0.6043619747675812 1107 84-85 0.6973754054157091 1107 86-87 0.7992915379615937 1107 88-89 1.019001026138799 1107 90-91 0.8639462546253966 1107 92-93 1.0069796652324179 1107 94-95 1.1058216307515387 1107 96-97 1.0854254041492517 1107 98-99 1.1070122372569493 1107 100-101 1.4700871946195981 1108 1 -0.0067987091309404946 1108 2 0.027346237552954733 1108 3 0.07679757946760901 1108 4 0.021941718327923354 1108 5 -0.053118522093761555 1108 6 -0.029210132188630666 1108 7 -0.043436513073906724 1108 8 -0.03688843496708216 1108 9 -0.03437171532654304 1108 10-11 0.012279358080846237 1108 12-13 0.03667944144784485 1108 14-15 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-2.3488007224753353 2316 98-99 -2.6994770751943555 2316 100-101 -3.0774841827361215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 14.0 5 43.0 6 97.0 7 237.0 8 430.0 9 820.0 10 1495.0 11 2490.0 12 3923.0 13 5963.0 14 8092.0 15 10987.0 16 15938.0 17 24379.0 18 24134.0 19 14038.0 20 10128.0 21 9581.0 22 9784.0 23 10457.0 24 11641.0 25 13469.0 26 15604.0 27 18357.0 28 22319.0 29 26821.0 30 32659.0 31 40492.0 32 51047.0 33 67086.0 34 97432.0 35 164191.0 36 212522.0 37 308002.0 38 223893.0 39 16151.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.12882062757463 19.85190459238163 15.777517842309582 19.241756937734152 2 15.442609098424414 21.971323029263583 40.8626827066731 21.723385165638902 3 19.189473819836206 28.463964596272874 30.711349902722485 21.635211681168443 4 11.872043940395667 18.799640610961365 37.75002119039143 31.57829425825154 5 11.948413363051909 39.58275554121011 33.25207513546612 15.216755960271861 6 27.826687217446555 38.274358336254785 17.870770132113126 16.02818431418553 7 25.647900456017226 36.98567529539405 20.178948617538865 17.187475631049857 8 26.524624268676718 34.24288205793084 19.408309622681937 19.824184050710507 9 27.83129057959959 16.9327840783875 20.510264625608166 34.725660716404754 10-11 24.28673142450287 28.769126447303734 25.577887402736106 21.36625472545729 12-13 26.676024343521675 26.950211056298635 27.043482683212122 19.33028191696757 14-15 23.32909525504755 26.851684212310772 28.077946735832104 21.741273796809573 16-17 19.475292003593893 30.68795877197444 28.502534370814896 21.334214853616775 18-19 19.390089677736526 27.320178338334266 33.92629134245368 19.36344064147553 20-21 21.232060214616286 25.8625845496618 34.23153469290885 18.673820542813065 22-23 20.405160284120768 26.520402108867756 33.93595416094526 19.138483446066214 24-25 19.643947559450286 26.69915240301662 34.53824629237773 19.118653745155363 26-27 19.545428648555525 26.88303135288237 34.3812780711595 19.19026192740261 28-29 19.20791092278164 27.12571636455857 34.57078815951904 19.09558455314075 30-31 19.36145996181657 27.238360275672015 34.320399722388636 19.079780040122778 32-33 19.130360779266397 27.29893962645177 34.07703694863051 19.49366264565133 34-35 19.59543983904676 27.254011707971465 33.99330130138684 19.157247151594934 36-37 19.703369500313812 27.04690879634449 33.933034516923456 19.316687186418243 38-39 19.49690632148144 27.227728312457288 33.91214258220842 19.363222783852844 40-41 19.586001031640013 27.205317049563828 33.9731061419207 19.23557577687546 42-43 19.40271985469511 27.38356923448387 33.55027783863046 19.663433072190568 44-45 19.387917722622074 27.701443061821962 33.21208919463465 19.69855002092131 46-47 19.674399475686595 27.896531509493013 32.40924112551074 20.019827889309656 48-49 19.774323337879956 28.08016841157358 32.03162904428331 20.113879206263153 50-51 20.087779077455117 28.38729254606791 31.665564766312364 19.859363610164607 52-53 20.061469087456985 28.684873450982384 31.35584600518741 19.897811456373223 54-55 19.666480191222092 28.67426130295479 31.35760904575429 20.301649460068827 56-57 19.911102069877433 28.88728406991134 30.87236603434539 20.32924782586584 58-59 19.891200054247403 28.457610740985608 31.153740527895028 20.49744867687196 60-61 19.89621793893777 28.548305616301345 31.30939666717524 20.246079777585653 62-63 20.03939717574463 28.547559714523047 31.305565444404888 20.107477665327433 64-65 19.777110986794145 28.509349200698438 31.349065079930156 20.36447473257726 66-67 19.671544762858613 28.746241239447585 31.09312504089755 20.48908895679625 68-69 19.890657394865812 28.81484482673859 30.992576569490193 20.301921208905405 70-71 19.770635203173473 28.848090321924424 30.70840326162505 20.672871213277052 72-73 19.815897879265627 28.86355083151096 30.567597348658225 20.75295394056519 74-75 19.792164640865245 29.085456610554512 30.301818983200256 20.820559765379986 76-77 19.770058824526608 28.79469053552357 30.764583227381376 20.670667412568445 78-79 19.819423960399398 28.84239434470833 30.695349980504837 20.642831714387427 80-81 19.616368503226877 29.241670890625187 30.456373995785647 20.68558661036229 82-83 19.733113448144685 29.260484610862207 30.222708344493725 20.78369359649938 84-85 19.766257905638557 28.906435689052284 30.2708862622185 21.05642014309066 86-87 19.8057315597893 28.939481064777397 30.272927866593612 20.981859508839694 88-89 19.845577884404133 28.881777200343926 30.149980606540616 21.122664308711325 90-91 19.63262842747336 28.989973994093575 30.371542011236453 21.00585556719662 92-93 19.765717443709462 28.89978741655167 30.307211901960716 21.027283237778146 94-95 19.481380746967144 28.83303209042237 30.253874709394672 21.431712453215805 96-97 19.62321661061083 28.66394976673538 30.092356515135076 21.620477107518717 98-99 19.59961226551065 28.71133886048238 29.935299604230092 21.753749269776883 100-101 19.86607263948789 28.502353052865708 30.18980388965742 21.441770417988987 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10214.0 1 6213.0 2 3510.5 3 4451.5 4 3090.5 5 2467.5 6 4682.5 7 10301.5 8 15570.0 9 16281.5 10 11752.0 11 6985.5 12 5912.0 13 5794.5 14 5757.0 15 5641.5 16 5348.0 17 5261.5 18 5436.0 19 5593.0 20 5751.0 21 6182.5 22 6879.0 23 7712.5 24 8720.5 25 10272.0 26 12290.5 27 14377.0 28 16009.5 29 18055.5 30 20565.5 31 22607.0 32 25335.5 33 28171.5 34 30844.5 35 33393.5 36 36197.5 37 39079.5 38 40474.0 39 41137.5 40 41759.0 41 42631.5 42 43780.5 43 45719.0 44 47461.0 45 48967.5 46 49159.5 47 49247.5 48 55273.0 49 55456.5 50 49642.0 51 45953.0 52 41676.0 53 39295.0 54 37353.5 55 34377.5 56 30912.5 57 27815.5 58 25387.0 59 22315.5 60 18524.5 61 15660.5 62 13054.0 63 9352.5 64 6211.5 65 4631.0 66 3427.5 67 2513.5 68 1912.5 69 1396.5 70 994.5 71 765.0 72 648.0 73 655.0 74 524.5 75 392.0 76 302.0 77 177.0 78 93.0 79 46.5 80 22.5 81 10.0 82 8.5 83 3.0 84 1.0 85 1.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01098509891674719 3 0.005289121700656055 4 0.0 5 1.3561850514502705E-4 6 2.712370102900541E-4 7 0.0 8 6.780925257251352E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017969451931716084 26-27 0.02352981064266219 28-29 3.051416365763108E-4 30-31 1.0171387885877028E-4 32-33 0.0037973181440607573 34-35 0.017596501042567256 36-37 0.0087812982081405 38-39 0.02356371526894845 40-41 0.025326755835833797 42-43 0.020885249792334165 44-45 0.010307006391022056 46-47 0.002339419213751716 48-49 3.051416365763108E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.3733238400379733E-4 68-69 1.695231314312838E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.695231314312838E-4 82-83 0.0010849480411602164 84-85 0.004441506043499636 86-87 8.815202834426758E-4 88-89 6.780925257251352E-5 90-91 0.004068555154350811 92-93 6.780925257251352E-4 94-95 1.0171387885877028E-4 96-97 3.390462628625676E-4 98-99 3.051416365763108E-4 100-101 0.0030514163657631085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1474725.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.54660210233385 #Duplication Level Percentage of deduplicated Percentage of total 1 73.92530511618001 38.10598288117355 2 14.465896908626721 14.913356640047255 3 5.025809643574984 7.771902298182959 4 2.3426052474503347 4.830133622926469 5 1.2254898722037504 3.158491941146334 6 0.7140406517627185 2.208382161678238 7 0.47276477445688814 1.7058592401850172 8 0.32193335582972993 1.3275656477139315 9 0.23252625689410047 1.0787344598218733 >10 1.1557601957835135 10.769864402920312 >50 0.07571089143894304 2.719316998354479 >100 0.03688750600287938 3.5564628308559865 >500 0.0035569663619654493 1.2306827880328115 >1k 0.0011856554539884832 1.8446320837677697 >5k 1.3173949488760924E-4 0.6314014546779828 >10k+ 3.952184846628277E-4 4.1472305485151395 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25396 1.7220837783315532 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21001 1.4240621132753564 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14675 0.995100781501636 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9298 0.6304904304192307 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4751 0.3221617589720117 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4205 0.2851379070674193 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3924 0.26608350709454304 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3331 0.22587262031904254 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2759 0.1870857278475648 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2393 0.16226754140602487 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2313 0.15684280120022376 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2008 0.13616097916560713 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1480 0.10035769380732 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.780925257251352E-5 0.0 10-11 0.0 0.0 0.0 6.780925257251352E-5 0.0 12-13 0.0 0.0 0.0 6.780925257251352E-5 0.0 14-15 0.0 0.0 0.0 1.3561850514502705E-4 0.0 16-17 0.0 0.0 0.0 2.712370102900541E-4 0.0 18-19 0.0 0.0 0.0 4.407601417213379E-4 0.0 20-21 0.0 0.0 0.0 8.47615657156419E-4 0.0 22-23 0.0 0.0 0.0 0.0016613266880265813 0.0 24-25 0.0 0.0 0.0 0.004034650528064555 0.0 26-27 0.0 0.0 0.0 0.007696350166980285 0.0 28-29 0.0 0.0 0.0 0.01644374374883453 0.0 30-31 0.0 0.0 0.0 0.03682042414687484 0.0 32-33 0.0 0.0 0.0 0.06679211378392581 0.0 34-35 0.0 0.0 0.0 0.10818966247944532 0.0 36-37 0.0 0.0 0.0 0.17342216345420333 0.0 38-39 0.0 0.0 0.0 0.26866025869229854 0.0 40-41 0.0 0.0 0.0 0.3718998457339504 0.0 42-43 0.0 0.0 0.0 0.47218973028869793 3.390462628625676E-5 44-45 0.0 0.0 0.0 0.5838376646493414 6.780925257251352E-5 46-47 0.0 0.0 0.0 0.7200325484412349 6.780925257251352E-5 48-49 0.0 0.0 0.0 0.8692129041007646 6.780925257251352E-5 50-51 0.0 0.0 0.0 1.0231399074403702 6.780925257251352E-5 52-53 0.0 0.0 0.0 1.1738120666564953 6.780925257251352E-5 54-55 0.0 0.0 0.0 1.3284171625218262 6.780925257251352E-5 56-57 0.0 0.0 0.0 1.4992286697519877 6.780925257251352E-5 58-59 0.0 0.0 0.0 1.6959094068385632 6.780925257251352E-5 60-61 0.0 0.0 0.0 1.9062537083185 6.780925257251352E-5 62-63 0.0 0.0 0.0 2.116021631151571 6.780925257251352E-5 64-65 0.0 0.0 0.0 2.321314143314855 6.780925257251352E-5 66-67 0.0 0.0 0.0 2.5379308006577497 6.780925257251352E-5 68-69 0.0 0.0 0.0 2.782891725575955 6.780925257251352E-5 70-71 0.0 0.0 0.0 3.056230822695757 6.780925257251352E-5 72-73 0.0 0.0 0.0 3.3300784892098525 6.780925257251352E-5 74-75 0.0 0.0 0.0 3.58887250165285 6.780925257251352E-5 76-77 0.0 0.0 0.0 3.8620759802675075 6.780925257251352E-5 78-79 0.0 0.0 0.0 4.162572683042601 6.780925257251352E-5 80-81 0.0 0.0 0.0 4.4893793758158305 6.780925257251352E-5 82-83 0.0 0.0 3.390462628625676E-5 4.824390988150332 6.780925257251352E-5 84-85 0.0 0.0 6.780925257251352E-5 5.1584532709488204 6.780925257251352E-5 86-87 0.0 0.0 6.780925257251352E-5 5.49621115801251 6.780925257251352E-5 88-89 0.0 0.0 6.780925257251352E-5 5.851802878502772 6.780925257251352E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12015 0.0 39.54109 1 GTATCAA 18620 0.0 32.607956 1 CAACGCG 70 0.008394619 27.141338 5 AACGCGG 70 0.008394619 27.141338 6 TATCAAC 23020 0.0 26.251501 2 ATCAACG 23160 0.0 26.090158 3 TCAACGC 23530 0.0 25.736977 4 CAACGCA 23780 0.0 25.386509 5 AACGCAG 24270 0.0 24.854397 6 GAGTACT 14875 0.0 23.948236 12-13 GTACATG 14495 0.0 22.877554 1 AGTACTT 15005 0.0 22.743645 12-13 GTACTTT 15530 0.0 22.724094 14-15 TACATGG 14545 0.0 22.341623 2 ACGCAGA 26940 0.0 22.285315 7 ATGGGCG 235 4.654794E-9 22.232798 5 CGCAGAG 27005 0.0 22.196499 8 GCAGAGT 27805 0.0 21.45537 9 ACATGGG 14795 0.0 20.838089 3 ACTTTTT 17320 0.0 20.471573 16-17 >>END_MODULE