FastQCFastQC Report
Thu 26 May 2016
SRR938193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481775
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267511.8053348180391762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209561.4142498017580267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146140.986249599298139No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA96570.6517183782963No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47240.31880683639553914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41570.2805419176325691No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41140.27763999257647076No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35620.2403873732516745No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.17735486156805183No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24540.16561218808523562No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23100.15589411347876703No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19360.13065411415363332No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15380.1037944357274215No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111050.040.8647881
GTATCAA180850.036.0692631
TATCAAC223050.029.0354272
ATCAACG224900.028.7754573
TCAACGC227900.028.3966674
CAACGCA230600.028.043585
AACGCAG235900.027.4538046
GAGTACT143950.025.12991512-13
ACGCAGA258950.024.9334667
CGCAGAG259350.024.7850178
GCAGAGT266700.023.7989069
GTATTAC2652.910383E-1123.4091031
GTACATG142950.023.333521
GTACTTT154550.023.2817814-15
TAGCCCT9000.023.2297614
TACATGG143500.022.6815782
AGTACTT146150.022.60497712-13
ACATGGG145700.021.3607433
ATGGGAG34550.021.1790875
TACCTGG11550.020.9808352
Produced by FastQC (version 0.11.5)