##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938193_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1481775 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.980970120294916 33.0 31.0 34.0 30.0 34.0 2 32.13877208078149 34.0 31.0 34.0 30.0 34.0 3 32.16657117308633 34.0 31.0 34.0 30.0 34.0 4 35.71751446744614 37.0 35.0 37.0 35.0 37.0 5 35.76546641696614 37.0 35.0 37.0 35.0 37.0 6 35.82962595535759 37.0 36.0 37.0 35.0 37.0 7 35.78989252754298 37.0 36.0 37.0 35.0 37.0 8 35.78221187427241 37.0 36.0 37.0 35.0 37.0 9 37.433722393750735 39.0 38.0 39.0 35.0 39.0 10-11 37.365414114828496 39.0 37.0 39.0 35.0 39.0 12-13 37.26448752340943 39.0 37.0 39.0 34.0 39.0 14-15 38.42429383678359 40.0 38.0 41.0 34.0 41.0 16-17 38.371428860656984 40.0 38.0 41.0 34.0 41.0 18-19 38.41601997604225 40.0 38.0 41.0 34.0 41.0 20-21 38.38300079296789 40.0 38.0 41.0 34.0 41.0 22-23 38.22077710853537 40.0 38.0 41.0 34.0 41.0 24-25 38.14708137200317 40.0 38.0 41.0 34.0 41.0 26-27 38.11506436537261 40.0 38.0 41.0 34.0 41.0 28-29 37.996278449832126 40.0 38.0 41.0 33.5 41.0 30-31 37.70211030689545 40.0 37.0 41.0 33.0 41.0 32-33 37.35173390022102 40.0 35.5 41.0 33.0 41.0 34-35 37.10566921428692 40.0 35.0 41.0 32.5 41.0 36-37 36.86040323260954 39.0 35.0 41.0 31.5 41.0 38-39 36.60418855764202 39.0 35.0 41.0 31.0 41.0 40-41 36.33907307114778 39.0 35.0 41.0 30.5 41.0 42-43 35.98965463717501 39.0 35.0 41.0 30.0 41.0 44-45 35.56813180138685 39.0 35.0 40.5 28.0 41.0 46-47 35.130591351588464 39.0 35.0 41.0 25.0 41.0 48-49 34.70358556461001 39.0 35.0 41.0 23.0 41.0 50-51 34.00756963776551 38.5 34.0 40.0 21.0 40.5 52-53 34.06676992120936 38.0 34.5 40.0 20.0 41.0 54-55 34.2059833645459 38.0 35.0 40.0 20.0 41.0 56-57 34.03030250881544 38.0 34.5 40.0 18.5 41.0 58-59 33.804436233571224 38.0 34.0 40.0 16.5 41.0 60-61 33.46988409171433 37.5 34.0 40.0 12.5 41.0 62-63 33.08303082451789 37.0 33.5 40.0 8.0 41.0 64-65 32.69496752205969 36.5 33.0 40.0 6.5 41.0 66-67 32.29614280170741 36.0 33.0 39.0 2.0 41.0 68-69 31.84909820991716 35.0 32.0 39.0 2.0 40.5 70-71 31.404290462452128 35.0 31.5 38.5 2.0 40.0 72-73 30.938562197364647 35.0 31.0 37.5 2.0 40.0 74-75 30.445792714818374 35.0 31.0 37.0 2.0 39.0 76-77 29.939509034772485 35.0 30.0 36.5 2.0 39.0 78-79 29.404690320730204 34.5 29.0 36.0 2.0 38.5 80-81 28.899715206424727 34.0 29.0 35.5 2.0 37.0 82-83 28.397869109682645 34.0 28.5 35.0 2.0 37.0 84-85 27.916099947697862 34.0 27.0 35.0 2.0 36.0 86-87 27.428341684803698 34.0 26.0 35.0 2.0 36.0 88-89 26.90354034856844 33.0 25.5 35.0 2.0 35.5 90-91 26.318619898432623 33.0 24.5 35.0 2.0 35.0 92-93 25.74293701810329 33.0 21.5 35.0 2.0 35.0 94-95 25.216436031111336 32.0 19.0 35.0 2.0 35.0 96-97 24.446938975215538 32.0 7.5 35.0 2.0 35.0 98-99 23.47131312108788 31.5 2.0 34.0 2.0 35.0 100-101 20.772603802871558 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4319865365015758 1101 2 0.3731472990924871 1101 3 0.3480023826786365 1101 4 0.28469180109626535 1101 5 0.3733471950188516 1101 6 0.3437984937218914 1101 7 0.39630417249478 1101 8 0.41717494894820817 1101 9 0.4444540861334829 1101 10-11 0.3717642721974457 1101 12-13 0.3480467025565943 1101 14-15 0.3517441492212683 1101 16-17 0.5427994476526408 1101 18-19 0.45807780188807357 1101 20-21 0.6543418651139632 1101 22-23 0.5620956948851088 1101 24-25 0.6324490973919765 1101 26-27 0.6510274921033385 1101 28-29 0.7546514525634294 1101 30-31 0.7395043104874119 1101 32-33 0.693069904205565 1101 34-35 0.6448465887520953 1101 36-37 0.7040358967857259 1101 38-39 0.8210295762689626 1101 40-41 0.7647290745413073 1101 42-43 0.8472548513659106 1101 44-45 0.9392768340161552 1101 46-47 0.9714605318170513 1101 48-49 0.8981656902565902 1101 50-51 1.1807722805909364 1101 52-53 1.0808745082736237 1101 54-55 1.1104220461728005 1101 56-57 0.9969745324111514 1101 58-59 0.952412452022088 1101 60-61 0.8424349814014889 1101 62-63 0.9357545887456027 1101 64-65 0.9495203820959901 1101 66-67 0.7379884430178976 1101 68-69 0.8592892896215396 1101 70-71 1.029272639227866 1101 72-73 1.0070262869795066 1101 74-75 0.8733970629215548 1101 76-77 0.8809758590232271 1101 78-79 0.7688422994563062 1101 80-81 0.9202912474023677 1101 82-83 0.8623989148044089 1101 84-85 0.9671908700768377 1101 86-87 1.0273231537134997 1101 88-89 0.8597013508112639 1101 90-91 0.8603745097335853 1101 92-93 1.0021407564011398 1101 94-95 1.0634243450143046 1101 96-97 1.0792472439096024 1101 98-99 1.257093421647955 1101 100-101 1.1894566283399506 1102 1 0.47434876123848824 1102 2 0.3525501468816046 1102 3 0.24725079872452937 1102 4 0.1267302759012594 1102 5 0.09642542147436473 1102 6 0.1960807794569277 1102 7 0.21202450096480874 1102 8 0.1760992519781439 1102 9 0.24204683897612966 1102 10-11 0.3062076341135551 1102 12-13 0.12480539085500197 1102 14-15 0.2360389492527375 1102 16-17 0.29451955827224907 1102 18-19 0.28096208095445974 1102 20-21 0.3582852603127762 1102 22-23 0.3969805064747547 1102 24-25 0.2813356396821902 1102 26-27 0.35126258901651397 1102 28-29 0.2918342835296599 1102 30-31 0.2741225578807658 1102 32-33 0.2900272599855569 1102 34-35 0.2785401821342788 1102 36-37 0.3048939853903292 1102 38-39 0.46420571438613223 1102 40-41 0.5251789356616712 1102 42-43 0.6487450087628446 1102 44-45 0.35995961354547745 1102 46-47 0.5171250058595689 1102 48-49 0.3325430919062029 1102 50-51 0.5872133913860367 1102 52-53 0.5211275173325731 1102 54-55 0.3947676779692699 1102 56-57 0.2834417852568336 1102 58-59 0.3102650520063506 1102 60-61 0.3011148078473198 1102 62-63 0.36113476798809074 1102 64-65 0.38121096188069004 1102 66-67 0.40305612793356715 1102 68-69 0.4299469558383784 1102 70-71 0.39271854878363754 1102 72-73 0.39460215641448926 1102 74-75 0.3352009249254131 1102 76-77 0.29295369879423916 1102 78-79 0.256597592918979 1102 80-81 0.14591287752865867 1102 82-83 0.2533460195117918 1102 84-85 0.3465601325926464 1102 86-87 0.46511505814978094 1102 88-89 0.41058182959807965 1102 90-91 0.518968103720912 1102 92-93 0.6522182143287765 1102 94-95 0.6178146361471875 1102 96-97 0.7470568184356239 1102 98-99 1.2499678974453765 1102 100-101 1.179011910910102 1103 1 0.3756818281151837 1103 2 0.3706993704348065 1103 3 0.31907477276851637 1103 4 0.16857493951934543 1103 5 0.07885339001681047 1103 6 0.15955075018587905 1103 7 0.22287242083972814 1103 8 0.21228543463416827 1103 9 0.11405067124951529 1103 10-11 0.2649898293243851 1103 12-13 0.24296607596203046 1103 14-15 0.3641194622601276 1103 16-17 0.2776989933058047 1103 18-19 0.3206613768036135 1103 20-21 0.2959158926378862 1103 22-23 0.4768046450949015 1103 24-25 0.5394507136826903 1103 26-27 0.3028554511134587 1103 28-29 0.31421517153558653 1103 30-31 0.42084190851027614 1103 32-33 0.38270156376963627 1103 34-35 0.4777331711899606 1103 36-37 0.5032047286867893 1103 38-39 0.4601603736547091 1103 40-41 0.38612883397711073 1103 42-43 0.4161639256166225 1103 44-45 0.5133401841545293 1103 46-47 0.5686687889952182 1103 48-49 0.6903495856111661 1103 50-51 0.519881464786998 1103 52-53 0.5702593781011487 1103 54-55 0.6843667586938835 1103 56-57 0.39410749581438864 1103 58-59 0.5260963967111181 1103 60-61 0.655625370110009 1103 62-63 0.5461668603388219 1103 64-65 0.7176842711545461 1103 66-67 0.7974634774345475 1103 68-69 0.9240824607932971 1103 70-71 1.069254690883394 1103 72-73 1.0079101959597843 1103 74-75 1.0196337837308285 1103 76-77 0.9694366475287701 1103 78-79 1.0958128585331401 1103 80-81 1.042191399164544 1103 82-83 1.0066986709512449 1103 84-85 1.202764065962814 1103 86-87 1.0644238382877873 1103 88-89 1.1467151127748672 1103 90-91 1.2092225502160296 1103 92-93 1.1665801290548181 1103 94-95 1.380737097266067 1103 96-97 1.2113155791771604 1103 98-99 1.4957183059469124 1103 100-101 1.164166259835067 1104 1 0.46328972240308275 1104 2 0.4337688050115105 1104 3 0.18333744204674218 1104 4 0.3117134350783246 1104 5 0.3121793090483109 1104 6 0.30402058081587313 1104 7 0.37009238157992286 1104 8 0.35455634890396226 1104 9 0.27758083630050834 1104 10-11 0.5137597210683893 1104 12-13 0.27996569157006945 1104 14-15 0.3613750008515737 1104 16-17 0.4330648783035116 1104 18-19 0.5088914245934646 1104 20-21 0.4612748888134064 1104 22-23 0.46706419604735316 1104 24-25 0.4808619828456031 1104 26-27 0.4968907892596093 1104 28-29 0.4657787074233184 1104 30-31 0.46770830423099596 1104 32-33 0.4241686561467972 1104 34-35 0.4640186923541947 1104 36-37 0.49059861599084087 1104 38-39 0.5148171850586252 1104 40-41 0.3690020666918912 1104 42-43 0.3487714311935406 1104 44-45 0.2078665398380295 1104 46-47 0.14035977889088969 1104 48-49 0.1342124332645369 1104 50-51 0.1962965886670247 1104 52-53 0.1820623707437079 1104 54-55 0.2227112062816019 1104 56-57 0.08455319613118917 1104 58-59 0.05713955658578129 1104 60-61 0.04286085769393111 1104 62-63 0.048099887598262114 1104 64-65 0.11311535161988218 1104 66-67 0.05381890178815496 1104 68-69 0.1799851988301775 1104 70-71 0.16210802375602995 1104 72-73 0.18825993865472057 1104 74-75 -0.03527326035979783 1104 76-77 0.04163188193227896 1104 78-79 0.08227518920092436 1104 80-81 0.11944149580241259 1104 82-83 0.2312521392248712 1104 84-85 0.20213457014253677 1104 86-87 0.28388249347740313 1104 88-89 0.386792185522129 1104 90-91 0.46253118240691293 1104 92-93 0.5964958111669887 1104 94-95 0.7008494887239287 1104 96-97 0.746302805702797 1104 98-99 0.7922340519804401 1104 100-101 0.6666726357747272 1105 1 0.3553431765625845 1105 2 0.3019203969593178 1105 3 0.28968308050947655 1105 4 0.06277233783649194 1105 5 0.25570498756327 1105 6 0.19136435450703715 1105 7 0.21909806647487784 1105 8 0.17947310368945324 1105 9 0.20649946570325284 1105 10-11 0.15508828712403044 1105 12-13 0.07642425012330989 1105 14-15 0.3858875412926963 1105 16-17 0.4949917367246144 1105 18-19 0.40715110240346775 1105 20-21 0.36462724399823543 1105 22-23 0.26920371128550613 1105 24-25 0.31599707540483024 1105 26-27 0.22668277960296734 1105 28-29 0.4041213744601819 1105 30-31 0.5064338204891712 1105 32-33 0.4513525753964771 1105 34-35 0.4394881835492086 1105 36-37 0.5011854282947965 1105 38-39 0.43886564942810224 1105 40-41 0.5876905927346954 1105 42-43 0.35939984149054993 1105 44-45 0.6046737338786272 1105 46-47 0.6600356296067673 1105 48-49 0.48973349612526107 1105 50-51 0.6157217683998937 1105 52-53 0.6791049749651421 1105 54-55 0.6494945540880863 1105 56-57 0.8665821739331179 1105 58-59 0.7175377256833428 1105 60-61 0.7439544172415822 1105 62-63 0.7158193034738289 1105 64-65 0.687869160420739 1105 66-67 0.7764954200410585 1105 68-69 0.7673562450516975 1105 70-71 0.8532072347233317 1105 72-73 0.9830533467758293 1105 74-75 0.9695528452607114 1105 76-77 0.9629366017537961 1105 78-79 1.0542543872506904 1105 80-81 1.085339510322818 1105 82-83 1.0287715696720419 1105 84-85 0.7858841295484105 1105 86-87 0.8393816004743364 1105 88-89 0.8236717846848514 1105 90-91 0.7838596795428039 1105 92-93 0.9694117271006419 1105 94-95 1.157093076725591 1105 96-97 1.0599908099246242 1105 98-99 0.9719319668625737 1105 100-101 0.8616072309302574 1106 1 -0.11321161943329017 1106 2 -0.0024476380484443894 1106 3 -0.07510112518381362 1106 4 -0.012526101098309539 1106 5 -0.08670054928671078 1106 6 0.02868821034940794 1106 7 -0.03819994166488527 1106 8 -0.04266692737313349 1106 9 -0.23658173916246739 1106 10-11 -0.5038679985157586 1106 12-13 -0.3249336045173692 1106 14-15 -0.2729723822978656 1106 16-17 0.012155518198163406 1106 18-19 -0.1344347901925218 1106 20-21 -0.13960134945593694 1106 22-23 -0.2007582540468249 1106 24-25 -0.12742989916693404 1106 26-27 -0.3132805461347772 1106 28-29 -0.2612259823854899 1106 30-31 -0.07861985884060374 1106 32-33 -0.1646219850225279 1106 34-35 -0.23989827361502591 1106 36-37 -0.3248768215890081 1106 38-39 -0.22992381649941507 1106 40-41 -0.10092819899857375 1106 42-43 -0.19379767803693682 1106 44-45 -0.09728553331633094 1106 46-47 -0.028376267138462197 1106 48-49 0.04144874060144588 1106 50-51 0.19201075251715594 1106 52-53 0.06632028179324578 1106 54-55 -0.1608890235513769 1106 56-57 0.028529934365728593 1106 58-59 0.1444003474112847 1106 60-61 -0.11463994387803211 1106 62-63 0.06673729370158554 1106 64-65 0.01073304052366808 1106 66-67 0.047481090437500484 1106 68-69 -0.17003676473187923 1106 70-71 -0.04295982305989199 1106 72-73 0.10132075713078237 1106 74-75 0.23767032760378015 1106 76-77 0.20721581362560215 1106 78-79 0.30438759361285506 1106 80-81 0.39875990960180374 1106 82-83 0.40997841931323364 1106 84-85 0.43962269364050854 1106 86-87 0.4071127843671327 1106 88-89 0.5153231467907595 1106 90-91 0.35841556386300155 1106 92-93 0.26303255944201354 1106 94-95 0.3975518167634533 1106 96-97 0.6579357475160279 1106 98-99 0.5529667819041251 1106 100-101 0.09857284668375144 1107 1 0.30231825054126205 1107 2 0.21979823489985506 1107 3 0.20415861601476593 1107 4 0.16050216464303446 1107 5 0.1748854059304179 1107 6 0.1760255402092099 1107 7 0.14428325962600042 1107 8 0.2788209621163915 1107 9 0.2996404634251917 1107 10-11 0.2184202672434381 1107 12-13 0.19751740501163795 1107 14-15 0.28569123565633703 1107 16-17 0.22852106203545475 1107 18-19 0.3341474514143741 1107 20-21 0.37888124673335 1107 22-23 0.43225008261099873 1107 24-25 0.5002949545332527 1107 26-27 0.41821878495129283 1107 28-29 0.42201359589975596 1107 30-31 0.5042431374118692 1107 32-33 0.46688854669042 1107 34-35 0.38761617787108804 1107 36-37 0.37757941787415206 1107 38-39 0.3110648998730454 1107 40-41 0.3055905212204806 1107 42-43 0.3419666821930676 1107 44-45 0.19762407182364683 1107 46-47 0.11987228503549119 1107 48-49 -0.009391395662440516 1107 50-51 0.027562345460495408 1107 52-53 0.07371492673336633 1107 54-55 0.09323378886801237 1107 56-57 0.09735488597099362 1107 58-59 0.02463864855732112 1107 60-61 3.4140774757673853E-4 1107 62-63 0.07401346189126912 1107 64-65 0.05666341992594681 1107 66-67 0.18359302709282588 1107 68-69 0.268807589393564 1107 70-71 0.2911292179076277 1107 72-73 0.3845259303031838 1107 74-75 0.28594837624930847 1107 76-77 0.273121239280659 1107 78-79 0.40016723310439417 1107 80-81 0.37775873420534367 1107 82-83 0.3008345577476277 1107 84-85 0.4002649848871549 1107 86-87 0.5128716915429443 1107 88-89 0.4978046523692079 1107 90-91 0.4787968671161771 1107 92-93 0.5485084254378947 1107 94-95 0.3979900245560586 1107 96-97 0.36001424472211596 1107 98-99 0.26505109808131166 1107 100-101 0.41410655432329335 1108 1 0.2508966816041749 1108 2 0.17899921693310716 1108 3 0.1522990905659114 1108 4 0.036125463087238074 1108 5 0.16216276926376594 1108 6 0.17465355721371623 1108 7 0.10895069692768544 1108 8 0.1114196655124502 1108 9 0.23032331763831593 1108 10-11 0.1628532714777151 1108 12-13 0.007954245576520691 1108 14-15 0.10153452302529331 1108 16-17 0.2543019616570845 1108 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-0.8962274849645908 2316 80-81 -1.0024316535225921 2316 82-83 -1.2393573222876029 2316 84-85 -1.35028573926542 2316 86-87 -1.4310403920318429 2316 88-89 -1.3178017787778948 2316 90-91 -1.2606359933509985 2316 92-93 -1.168400183292384 2316 94-95 -1.3648092998610863 2316 96-97 -1.5482853896625954 2316 98-99 -1.4957719651792694 2316 100-101 -1.5166270262867556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4142.0 3 907.0 4 877.0 5 990.0 6 1217.0 7 1445.0 8 1871.0 9 2464.0 10 3107.0 11 4100.0 12 5934.0 13 8153.0 14 9549.0 15 12644.0 16 17641.0 17 27088.0 18 22785.0 19 13610.0 20 12058.0 21 11453.0 22 11779.0 23 12640.0 24 13754.0 25 15364.0 26 17090.0 27 19973.0 28 23185.0 29 27465.0 30 32980.0 31 40812.0 32 51246.0 33 66778.0 34 95243.0 35 159585.0 36 203363.0 37 275392.0 38 225682.0 39 27408.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.80562325049191 19.486103301606207 15.513350692477937 19.194922755423942 2 15.247025238294903 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21.411636544635325 25.78637462799818 34.20789148707191 18.59409734029459 22-23 20.538644596131352 26.50594802367324 33.902451871520526 19.052955508674877 24-25 19.682235610916106 26.718741398229508 34.471589966878014 19.127433023976376 26-27 19.558602265268142 26.818717770712507 34.39477821387956 19.22790175013979 28-29 19.199040772244523 27.123407379043872 34.54374720796448 19.133804640747123 30-31 19.45348179538912 27.116453794139744 34.34926852328653 19.080795887184596 32-33 19.102973205693157 27.259585675935778 34.07080990730892 19.56663121106215 34-35 19.59651045192923 27.227828588698312 33.98902213975414 19.186638819618324 36-37 19.6792608155098 27.075326503089276 33.895035478235265 19.350377203165657 38-39 19.58004187901266 27.158603848372724 33.896716214158864 19.364638058455753 40-41 19.62005681424981 27.257168030760038 33.915440276678304 19.207334878311844 42-43 19.378020230799223 27.35853546972554 33.56675437959839 19.696689919876853 44-45 19.436645292546668 27.698436243325443 33.21304695455072 19.65187150957718 46-47 19.67495365738734 27.909863787146662 32.449819692471365 19.96536286299463 48-49 19.74436406434743 28.115092537994528 32.02277359826025 20.117769799397784 50-51 20.132564372666682 28.43228352223768 31.62734951921957 19.80780258587607 52-53 20.01298773185869 28.656073519742836 31.404322133552228 19.926616614846246 54-55 19.607587019967955 28.60496348551723 31.478464437489308 20.308985057025506 56-57 19.928223374959455 28.894934965301438 30.95838636588393 20.21845529385518 58-59 19.940261230551148 28.435755509804583 31.24209389449526 20.381889365149007 60-61 19.860073250924074 28.410098515100955 31.56580853973058 20.16401969424439 62-63 20.088168201330216 28.351591458983933 31.526915702573056 20.03332463711279 64-65 19.81755154292102 28.39298003084121 31.609740259521104 20.179728166716675 66-67 19.705684029490993 28.566819458440797 31.406033058911113 20.321463453157094 68-69 19.931103541448067 28.65811203993794 31.234692441470578 20.17609197714341 70-71 19.79111987736842 28.700644652978745 30.983614802882425 20.524620666770414 72-73 19.834798899559487 28.748063258451616 30.79282350756975 20.624314334419147 74-75 19.767645168607288 28.96435727247197 30.715946404268646 20.552051154652094 76-77 19.871092380194114 28.696340102271527 30.841774114996234 20.590793402538125 78-79 19.915872146451584 28.789457289623837 30.786717599656306 20.507952964268274 80-81 19.807043222736596 29.150296974702055 30.486995312595443 20.555664489965903 82-83 19.88104965478277 29.169969999159612 30.26335196024728 20.685628385810332 84-85 20.006972646369043 28.803171912857444 30.284842391529953 20.905013049243557 86-87 20.036581332721397 28.83348069356047 30.295451630973997 20.834486342744142 88-89 20.17723037619681 28.578912954169294 30.339401807816003 20.904454861817896 90-91 20.02630633647803 28.787882880084698 30.388173762265264 20.79763702117201 92-93 20.149808410495705 28.7263753534077 30.338279015886133 20.785537220210465 94-95 19.921275606613012 28.629710068842485 30.356596046089457 21.092418278455046 96-97 20.11620707977388 28.421684786608235 30.14210589211484 21.32000224150305 98-99 20.194336515343146 28.3105711786938 29.985185275196425 21.509907030766627 100-101 20.64892647228324 27.963030126242305 30.278751144576827 21.10929225689763 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10805.0 1 6510.5 2 3541.0 3 4374.5 4 2949.5 5 2509.0 6 4779.0 7 10284.0 8 15749.5 9 16329.5 10 11578.5 11 7065.0 12 6065.0 13 5974.0 14 5858.5 15 5638.5 16 5435.5 17 5331.5 18 5462.5 19 5602.0 20 5848.5 21 6381.0 22 7026.5 23 7873.5 24 8971.5 25 10543.0 26 12543.0 27 14650.5 28 16152.5 29 18211.5 30 20806.5 31 22752.0 32 25580.5 33 28589.0 34 31065.0 35 33323.0 36 36347.0 37 39141.0 38 40467.5 39 41379.0 40 41819.0 41 42830.5 42 44033.0 43 45387.0 44 47321.5 45 48771.5 46 48456.5 47 49085.5 48 55467.0 49 55612.5 50 49599.0 51 45660.5 52 41360.5 53 39036.0 54 37345.5 55 34562.5 56 31082.5 57 27926.0 58 25241.5 59 22305.0 60 18949.0 61 16070.0 62 13331.0 63 9534.5 64 6439.0 65 4971.5 66 3712.5 67 2743.5 68 2095.5 69 1559.0 70 1133.0 71 874.5 72 751.0 73 717.5 74 583.5 75 439.0 76 325.5 77 208.0 78 132.0 79 70.5 80 36.5 81 31.0 82 20.0 83 11.0 84 7.5 85 7.0 86 8.5 87 6.0 88 2.5 89 1.5 90 1.5 91 3.0 92 2.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.501560628300518 2 0.04940021258288201 3 0.055743955728771236 4 0.06411229775100809 5 0.07943176258203843 6 0.10824855325538628 7 0.06741914258237586 8 0.05169475797607599 9 0.050547485279479006 10-11 0.07386411567208247 12-13 0.10602149449140388 14-15 0.05348315365018305 16-17 0.059793153481466486 18-19 0.058342190953417354 20-21 0.07447149533498676 22-23 0.06390983786337331 24-25 0.0903308531997098 26-27 0.06606940999814412 28-29 0.06752037252619325 30-31 0.07001737780702198 32-33 0.07086096067216682 34-35 0.0720419766833696 36-37 0.06802652224528015 38-39 0.04072818072919303 40-41 0.042685292976329066 42-43 0.041672993538155255 44-45 0.020954598370197905 46-47 0.028141924381231972 48-49 0.02770326129135665 50-51 0.025746149044220613 52-53 0.025442459212768474 54-55 0.025408715898162675 56-57 0.025206256010527915 58-59 0.021933154493765924 60-61 0.028918020617165224 62-63 0.01946989252754298 64-65 0.021292031516255843 66-67 0.016061817752357814 68-69 0.018660052977003933 70-71 0.03985085454944239 72-73 0.0371176460663731 74-75 0.031246309324964992 76-77 0.022034384437583304 78-79 0.014880801741155034 80-81 0.016972887246714245 82-83 0.02135951814546743 84-85 0.018322619830945994 86-87 0.009785561235680182 88-89 0.04018828769550033 90-91 0.051492298088441225 92-93 0.03259604190919674 94-95 0.05301074724570194 96-97 0.042246629886453745 98-99 0.0322923520777446 100-101 0.16983010241095983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1481775.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.93959743416018 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2252407672347 39.82393963105179 2 13.798049590156108 14.609263813588862 3 4.885645950187835 7.759323894263368 4 2.134248738189661 4.5194507609649985 5 1.1422425229802107 3.02349296693759 6 0.673521705152989 2.1393580760381017 7 0.4310517731296696 1.5973795139935958 8 0.30507042170156945 1.2920244251160455 9 0.21526023393919486 1.0256211115491707 >10 1.0775519620680332 10.296778766490347 >50 0.07091724076798638 2.5464493179853487 >100 0.03660731482282995 3.5871219093922915 >500 0.002933637010901667 1.067827027565056 >1k 0.0011479449173093478 1.8517149726139202 >5k 1.2754943525659422E-4 0.6520807895027031 >10k+ 3.826483057697826E-4 4.208173022946874 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26751 1.8053348180391762 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20956 1.4142498017580267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14614 0.986249599298139 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9657 0.6517183782963 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4724 0.31880683639553914 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4157 0.2805419176325691 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4114 0.27763999257647076 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3562 0.2403873732516745 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2628 0.17735486156805183 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2454 0.16561218808523562 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2310 0.15589411347876703 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1936 0.13065411415363332 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1538 0.1037944357274215 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.3743314605793726E-5 0.0 12-13 0.0 0.0 0.0 1.349732584231749E-4 0.0 14-15 0.0 0.0 0.0 1.6871657302896864E-4 0.0 16-17 0.0 0.0 0.0 3.374331460579373E-4 0.0 18-19 0.0 0.0 0.0 5.736363482984933E-4 0.0 20-21 0.0 0.0 0.0 7.42352921327462E-4 0.0 22-23 0.0 0.0 0.0 0.0014509625280491303 0.0 24-25 0.0 0.0 0.0 0.0036780212920315162 0.0 26-27 0.0 0.0 0.0 0.007288555954851445 0.0 28-29 0.0 0.0 0.0 0.01656796747144472 0.0 30-31 0.0 0.0 0.0 0.03600411668438191 0.0 32-33 0.0 0.0 0.0 0.07113090718901319 0.0 34-35 0.0 0.0 0.0 0.11388368679455382 0.0 36-37 0.0 0.0 0.0 0.17664625196133016 0.0 38-39 0.0 0.0 0.0 0.2695415970710803 0.0 40-41 0.0 0.0 0.0 0.37191881358505846 0.0 42-43 0.0 0.0 0.0 0.4701793457171298 0.0 44-45 0.0 0.0 0.0 0.5847716421184053 0.0 46-47 0.0 0.0 0.0 0.7220057026201684 0.0 48-49 0.0 0.0 0.0 0.8697339339643333 0.0 50-51 0.0 0.0 0.0 1.0268428067689088 0.0 52-53 0.0 0.0 0.0 1.1703193804727439 0.0 54-55 0.0 0.0 0.0 1.3235140287830474 0.0 56-57 0.0 0.0 0.0 1.491893168665958 0.0 58-59 0.0 0.0 0.0 1.6798096877056232 0.0 60-61 0.0 0.0 0.0 1.8979602166320797 0.0 62-63 0.0 0.0 0.0 2.1062576976936445 0.0 64-65 0.0 0.0 0.0 2.3077052858902327 0.0 66-67 0.0 0.0 0.0 2.5296350660525384 0.0 68-69 0.0 0.0 0.0 2.775623829528775 0.0 70-71 0.0 0.0 0.0 3.047122538846991 0.0 72-73 0.0 0.0 0.0 3.314538307097906 0.0 74-75 0.0 0.0 0.0 3.578782203775877 0.0 76-77 0.0 0.0 0.0 3.851563159049113 0.0 78-79 0.0 0.0 0.0 4.151473739265408 0.0 80-81 0.0 0.0 0.0 4.47530832953721 0.0 82-83 0.0 0.0 0.0 4.81095307992104 3.3743314605793726E-5 84-85 0.0 0.0 0.0 5.146226653844208 6.748662921158745E-5 86-87 0.0 0.0 0.0 5.485448195576252 6.748662921158745E-5 88-89 0.0 0.0 0.0 5.839483052420239 6.748662921158745E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11105 0.0 40.864788 1 GTATCAA 18085 0.0 36.069263 1 TATCAAC 22305 0.0 29.035427 2 ATCAACG 22490 0.0 28.775457 3 TCAACGC 22790 0.0 28.396667 4 CAACGCA 23060 0.0 28.04358 5 AACGCAG 23590 0.0 27.453804 6 GAGTACT 14395 0.0 25.129915 12-13 ACGCAGA 25895 0.0 24.933466 7 CGCAGAG 25935 0.0 24.785017 8 GCAGAGT 26670 0.0 23.798906 9 GTATTAC 265 2.910383E-11 23.409103 1 GTACATG 14295 0.0 23.33352 1 GTACTTT 15455 0.0 23.28178 14-15 TAGCCCT 900 0.0 23.229761 4 TACATGG 14350 0.0 22.681578 2 AGTACTT 14615 0.0 22.604977 12-13 ACATGGG 14570 0.0 21.360743 3 ATGGGAG 3455 0.0 21.179087 5 TACCTGG 1155 0.0 20.980835 2 >>END_MODULE