FastQCFastQC Report
Thu 26 May 2016
SRR938193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481775
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256631.7319093654569688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215841.4566314049029037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146130.9861821126689275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94480.6376136727910783No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49210.33210170235022185No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45250.3053769971824332No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40760.27507550066643044No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33300.2247304752745862No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26050.17580266909618533No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23610.159335931568558No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23180.1564340065124597No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19950.13463582527711696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112550.040.558811
GTATCAA179900.034.748181
TATCAAC223200.027.9858762
ATCAACG225750.027.6258093
TCAACGC229200.027.2505044
CAACGCA230550.027.0909355
AACGCAG236550.026.48416
TAAGGTG6150.026.2583355
GTAAGGT5150.024.9011764
GAGTACT143900.024.49099712-13
ACGCAGA262950.023.6986737
CGCAGAG263450.023.6717248
GTACTTT152000.023.02964614-15
GTACATG142600.022.8513531
AGTACTT144800.022.764312-13
GCAGAGT273050.022.613339
TACATGG144600.022.2067872
ACATGGG142850.021.380053
ACTTTTT166450.021.17305416-17
CGGTATA1404.8516155E-420.3577862
Produced by FastQC (version 0.11.5)