##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938193_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1481775 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52768267786945 33.0 31.0 34.0 31.0 34.0 2 32.7101732719205 34.0 31.0 34.0 31.0 34.0 3 32.7429319566061 34.0 31.0 34.0 31.0 34.0 4 36.21685546051189 37.0 35.0 37.0 35.0 37.0 5 36.20784599551214 37.0 35.0 37.0 35.0 37.0 6 36.27295911997435 37.0 37.0 37.0 35.0 37.0 7 36.242449764640384 37.0 37.0 37.0 35.0 37.0 8 36.24703953029306 37.0 37.0 37.0 35.0 37.0 9 37.93141806279631 39.0 38.0 39.0 35.0 39.0 10-11 37.934012923689494 39.0 38.0 39.0 35.0 39.0 12-13 37.878920213932616 39.0 38.0 39.0 35.0 39.0 14-15 39.09315078200132 40.5 38.5 41.0 36.0 41.0 16-17 39.07220934352382 40.0 38.5 41.0 36.0 41.0 18-19 39.18649120143071 40.0 39.0 41.0 36.0 41.0 20-21 39.1772050412512 40.0 39.0 41.0 36.0 41.0 22-23 39.022799007946546 40.0 38.5 41.0 35.0 41.0 24-25 38.9607538256483 40.0 38.0 41.0 35.0 41.0 26-27 38.87091292537666 40.0 38.0 41.0 35.0 41.0 28-29 38.77518921563666 40.0 38.0 41.0 35.0 41.0 30-31 38.47205817347438 40.0 37.5 41.0 35.0 41.0 32-33 38.16198073256736 40.0 37.0 41.0 34.0 41.0 34-35 37.92138178873311 40.0 36.0 41.0 34.0 41.0 36-37 37.748940966071096 40.0 36.0 41.0 33.5 41.0 38-39 37.662354271060046 40.0 36.0 41.0 33.0 41.0 40-41 37.53072497511431 40.0 36.0 41.0 33.0 41.0 42-43 37.29054681041319 40.0 35.0 41.0 33.0 41.0 44-45 36.933082620505814 40.0 35.0 41.0 32.5 41.0 46-47 36.382619830946 40.0 35.0 41.0 30.5 41.0 48-49 35.86615376828466 40.0 35.0 41.0 29.0 41.0 50-51 35.512045013581684 39.0 35.0 41.0 27.0 41.0 52-53 35.10492584906615 39.0 35.0 41.0 26.0 41.0 54-55 34.91589175144675 39.0 35.0 41.0 25.0 41.0 56-57 34.69960992728316 38.5 35.0 40.5 24.0 41.0 58-59 34.410722275649135 38.0 35.0 40.0 23.0 41.0 60-61 34.09117646066373 38.0 34.0 40.0 22.0 41.0 62-63 33.76328524911002 37.0 34.0 40.0 21.5 41.0 64-65 33.40753150782001 37.0 34.0 40.0 20.0 41.0 66-67 33.01946381873091 36.0 33.0 39.0 18.5 41.0 68-69 32.60301462772688 36.0 33.0 39.0 15.5 40.5 70-71 32.134147896947916 35.0 32.5 38.5 10.5 40.0 72-73 31.74195103845051 35.0 32.0 37.5 7.5 40.0 74-75 31.317879232677026 35.0 31.5 37.0 6.5 39.0 76-77 29.989099559649745 33.5 30.0 35.5 2.0 38.5 78-79 30.686946061311602 35.0 31.0 36.0 2.0 39.0 80-81 30.5203509304719 35.0 31.0 36.0 2.0 37.5 82-83 30.174371615123754 35.0 31.0 35.0 2.0 37.0 84-85 29.813670429046244 34.5 31.0 35.0 2.0 37.0 86-87 29.399627811239895 34.0 30.5 35.0 2.0 36.0 88-89 28.964287425553813 34.0 29.5 35.0 2.0 36.0 90-91 28.648232356464376 34.0 29.0 35.0 2.0 35.5 92-93 28.297501982419732 34.0 29.0 35.0 2.0 35.0 94-95 27.962809468374076 34.0 29.0 35.0 2.0 35.0 96-97 27.467518347927317 34.0 28.0 35.0 2.0 35.0 98-99 26.88607852069309 34.0 26.5 35.0 2.0 35.0 100-101 24.683787687064502 31.0 20.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1633352912182886 1101 2 0.1567559862423522 1101 3 0.22079572754353904 1101 4 0.050459989512070536 1101 5 0.11888866188403568 1101 6 0.035516276833341465 1101 7 0.113164371598792 1101 8 0.12380285324316986 1101 9 0.17211826465596403 1101 10-11 0.10455818175073972 1101 12-13 0.1961301700891127 1101 14-15 0.14903746562958275 1101 16-17 0.16413377083046754 1101 18-19 0.1505921685285685 1101 20-21 0.10244270617494067 1101 22-23 0.06352006472286575 1101 24-25 0.16660602214864895 1101 26-27 0.11162509895859785 1101 28-29 0.116831486940292 1101 30-31 0.2655972770911674 1101 32-33 0.37062529723677073 1101 34-35 0.21889661810535443 1101 36-37 0.2919884634253833 1101 38-39 0.18230574349008322 1101 40-41 0.30447662464882086 1101 42-43 0.17546866574327424 1101 44-45 0.17185503090100696 1101 46-47 0.020169877194163632 1101 48-49 -0.05221100555822744 1101 50-51 0.05960735529929906 1101 52-53 0.16158802842058861 1101 54-55 0.21343672680360726 1101 56-57 0.15977937540903042 1101 58-59 0.1520690322590852 1101 60-61 0.13923129390808953 1101 62-63 0.09560708893568659 1101 64-65 0.18462474182872057 1101 66-67 0.042264462616827814 1101 68-69 0.09194105706032474 1101 70-71 0.17855970169691915 1101 72-73 0.08276317950294043 1101 74-75 0.1445357172565771 1101 76-77 0.1337611685510609 1101 78-79 0.12404710932545271 1101 80-81 0.22766842707996204 1101 82-83 0.32052149135619956 1101 84-85 0.3479073839168052 1101 86-87 0.4004284487208416 1101 88-89 0.5191360737197215 1101 90-91 0.6555813538066992 1101 92-93 0.8187929808625505 1101 94-95 0.6398591326460128 1101 96-97 0.5897681326241866 1101 98-99 0.4554135680371907 1101 100-101 0.7727882561054891 1102 1 0.11252712666860276 1102 2 0.16444852668083598 1102 3 0.16942175703806583 1102 4 0.10515073268457797 1102 5 0.06545782031575698 1102 6 0.05211042754952899 1102 7 0.08232038275757247 1102 8 0.09842146372056249 1102 9 0.09672507490219573 1102 10-11 0.061021682728046756 1102 12-13 0.06390888247209858 1102 14-15 0.23407507508429148 1102 16-17 0.2806813257620604 1102 18-19 0.18160589915652992 1102 20-21 0.06765471699483783 1102 22-23 0.2632147715627582 1102 24-25 0.12236725808670457 1102 26-27 -0.04751947505305054 1102 28-29 0.11948442096187506 1102 30-31 0.17008378549353154 1102 32-33 0.14227176231111116 1102 34-35 0.3345860844323383 1102 36-37 0.3819334561939627 1102 38-39 0.5005101719050487 1102 40-41 0.5039922100310505 1102 42-43 0.38793455097241036 1102 44-45 0.5000000950761105 1102 46-47 0.43883179149423057 1102 48-49 0.5768835233704905 1102 50-51 0.6422104862939619 1102 52-53 0.5626210025024321 1102 54-55 0.5046431340265656 1102 56-57 0.625876100996166 1102 58-59 0.630775061269496 1102 60-61 0.6884930230352992 1102 62-63 0.5122853541201735 1102 64-65 0.8333832969963169 1102 66-67 0.5528765012731753 1102 68-69 0.5411891705361924 1102 70-71 0.6360682795572572 1102 72-73 0.4679091090283265 1102 74-75 0.6080003861541918 1102 76-77 0.8230277993127473 1102 78-79 0.7609276728318761 1102 80-81 0.7251970350762775 1102 82-83 0.6474245055588348 1102 84-85 0.7122906554249973 1102 86-87 0.5018868362646423 1102 88-89 0.37676144361158137 1102 90-91 0.6037265995238208 1102 92-93 0.5131037565915939 1102 94-95 0.6411656300348341 1102 96-97 0.5828734693543716 1102 98-99 0.569347523051082 1102 100-101 0.8607483894783563 1103 1 0.1247874626390697 1103 2 0.13966629620368565 1103 3 0.12758649777183706 1103 4 0.07496981646559675 1103 5 0.03431766020666771 1103 6 0.06550692677954828 1103 7 0.06037885431528878 1103 8 0.0870757009688532 1103 9 0.09088673571073258 1103 10-11 0.010439788456466204 1103 12-13 0.03968415372886369 1103 14-15 0.17442532841402425 1103 16-17 0.13919637860276168 1103 18-19 0.1113924203692207 1103 20-21 0.10334717117801517 1103 22-23 0.20770999297647563 1103 24-25 0.11349337044519814 1103 26-27 0.26648125378421383 1103 28-29 0.2697448039541044 1103 30-31 0.14393851176811978 1103 32-33 0.20295393048444055 1103 34-35 0.15272090757174084 1103 36-37 0.1885035715148291 1103 38-39 0.1075910610865165 1103 40-41 0.1996080187465381 1103 42-43 0.317682279233658 1103 44-45 0.22372551151256204 1103 46-47 0.20350728257772488 1103 48-49 0.32680270472166484 1103 50-51 0.2691912779052785 1103 52-53 0.3009837048429418 1103 54-55 0.409729125068921 1103 56-57 0.4290368448804003 1103 58-59 0.3116495484384316 1103 60-61 0.26615709958672795 1103 62-63 0.43518436313659237 1103 64-65 0.34899011280032965 1103 66-67 0.28192771972450714 1103 68-69 0.35154424359624414 1103 70-71 0.3434812599139221 1103 72-73 0.36926186626673996 1103 74-75 0.38775340436336947 1103 76-77 0.3245545370606422 1103 78-79 0.4062390156557676 1103 80-81 0.3543134962186727 1103 82-83 0.40120564278008075 1103 84-85 0.6104941303330094 1103 86-87 0.6041950168752201 1103 88-89 0.3459134061822624 1103 90-91 0.49211281274398644 1103 92-93 0.47876641073479576 1103 94-95 0.33034533544351063 1103 96-97 0.44601164279413297 1103 98-99 0.5736952724721256 1103 100-101 0.8225667209112295 1104 1 0.10014696629835385 1104 2 0.10172000742166176 1104 3 0.08552581135145942 1104 4 0.10809446959504498 1104 5 0.08702686768978651 1104 6 0.020824600819480565 1104 7 0.04576008259876119 1104 8 0.06769467999789214 1104 9 0.038785968870371335 1104 10-11 -0.04432148902053967 1104 12-13 0.14801871078191908 1104 14-15 0.09086299753028726 1104 16-17 0.07672296751283625 1104 18-19 0.14605410145778563 1104 20-21 0.2195906467723603 1104 22-23 0.10771598560391737 1104 24-25 0.13564800614744144 1104 26-27 0.10739912995386192 1104 28-29 0.13132806692355103 1104 30-31 0.006152350310536292 1104 32-33 0.10370252302679717 1104 34-35 0.34563789186565685 1104 36-37 0.41746189090135744 1104 38-39 0.44722781068538353 1104 40-41 0.3547549838856412 1104 42-43 0.3147761248592573 1104 44-45 0.3674918832627796 1104 46-47 0.3612683289876557 1104 48-49 0.45052589716166125 1104 50-51 0.5353845989870152 1104 52-53 0.4700276270230077 1104 54-55 0.334504374545773 1104 56-57 0.3369952160950547 1104 58-59 0.41153369097442294 1104 60-61 0.42439971569793045 1104 62-63 0.38276398949897583 1104 64-65 0.5595453972179882 1104 66-67 0.5676961473238578 1104 68-69 0.7637978710254245 1104 70-71 0.5709592383577373 1104 72-73 0.6219949902720145 1104 74-75 0.5434726384299964 1104 76-77 0.4811799535752428 1104 78-79 0.5566831179385794 1104 80-81 0.5016916407284526 1104 82-83 0.4928215790660353 1104 84-85 0.4241954901735525 1104 86-87 0.26871639489879584 1104 88-89 0.23322339670203363 1104 90-91 0.1999484700650349 1104 92-93 0.2520337315876269 1104 94-95 0.27730734326523887 1104 96-97 0.267494780296321 1104 98-99 0.32726493487664854 1104 100-101 0.5467330978442533 1105 1 0.10609671001533627 1105 2 0.09415557237656458 1105 3 0.05452964525879622 1105 4 -0.05496867861983645 1105 5 0.08743743249699776 1105 6 0.11079293711636495 1105 7 0.0025109692021416663 1105 8 -0.03108406200003344 1105 9 0.027573709818746295 1105 10-11 0.04999248376994814 1105 12-13 -0.030369236531882393 1105 14-15 -0.025461482568644556 1105 16-17 0.03654496748526981 1105 18-19 -0.07774039362626439 1105 20-21 0.11527051058693161 1105 22-23 -0.08309007027621362 1105 24-25 3.324422952530881E-4 1105 26-27 -0.03153669000411696 1105 28-29 -0.09831820737311858 1105 30-31 0.007665385004735015 1105 32-33 -0.10933852163910274 1105 34-35 -0.10219157900787224 1105 36-37 -0.03285066017309646 1105 38-39 -0.16975116091376918 1105 40-41 -0.18634260254464863 1105 42-43 -0.35008090603844977 1105 44-45 -0.2531612495337683 1105 46-47 -0.28580322152954096 1105 48-49 -0.22552640899169063 1105 50-51 -0.14361156513650997 1105 52-53 -0.0635109394855391 1105 54-55 -0.11041914422106913 1105 56-57 -0.21803917486109725 1105 58-59 -0.2668586868948566 1105 60-61 -0.16932203951092362 1105 62-63 -0.15173502297944452 1105 64-65 -0.14057319398352064 1105 66-67 -0.156533034480546 1105 68-69 0.0254017419525141 1105 70-71 0.01591869385231348 1105 72-73 -0.16722182535977126 1105 74-75 -0.1165858547479317 1105 76-77 -0.07592547592280496 1105 78-79 -0.06844118375665076 1105 80-81 -0.1520336195420633 1105 82-83 -0.052193356828748705 1105 84-85 -0.1690317658160687 1105 86-87 -0.16333783706933858 1105 88-89 -0.38242566982915704 1105 90-91 -0.27683074255633855 1105 92-93 -0.08715162710029745 1105 94-95 -0.1419514444954011 1105 96-97 0.05650409824676572 1105 98-99 0.1508323393784856 1105 100-101 0.2746642548887408 1106 1 -0.24433238452150619 1106 2 -0.06423529476851542 1106 3 -0.014680979507389225 1106 4 -0.08105445218036067 1106 5 -0.10656771437010093 1106 6 -0.059572331733754424 1106 7 0.003519936541074742 1106 8 -0.28923232351529293 1106 9 -0.2466726822727665 1106 10-11 -0.07415442867662847 1106 12-13 -0.23353444643110066 1106 14-15 -0.40652662331391554 1106 16-17 -0.23081862353151905 1106 18-19 -0.20239462392023455 1106 20-21 -0.17647267888352758 1106 22-23 -0.1755225318164193 1106 24-25 -0.13164388665077098 1106 26-27 -0.23601979263537487 1106 28-29 -0.20508310664644114 1106 30-31 -0.1614315892269147 1106 32-33 -0.03767491588459393 1106 34-35 -0.20509916213431012 1106 36-37 -0.1546111948124249 1106 38-39 -0.0642942347497737 1106 40-41 -0.010636325407396896 1106 42-43 -0.2385985176200336 1106 44-45 -0.18110936140443812 1106 46-47 -0.06018423707217124 1106 48-49 0.027368703751911028 1106 50-51 -0.10530623348100931 1106 52-53 -0.19801960640160843 1106 54-55 -0.27177597855769875 1106 56-57 -0.16305895742296883 1106 58-59 -0.14704381539647926 1106 60-61 -0.06401107350972524 1106 62-63 -0.02663582675230458 1106 64-65 -0.396730445511146 1106 66-67 -0.44125596524725097 1106 68-69 -0.4521728252685264 1106 70-71 -0.4356692960353392 1106 72-73 -0.24436531411942042 1106 74-75 -0.26970373387591096 1106 76-77 -0.2446412532244402 1106 78-79 -0.2966265163190869 1106 80-81 -0.3236161678081153 1106 82-83 -0.3328421568378168 1106 84-85 -0.32732627091192157 1106 86-87 -0.13717979197189578 1106 88-89 -0.142290026073983 1106 90-91 -0.16682070956650463 1106 92-93 -0.2186333919995711 1106 94-95 -0.3490037294096382 1106 96-97 -0.14043867403270838 1106 98-99 -0.08277760971967751 1106 100-101 0.13050521216067423 1107 1 0.13172263866959355 1107 2 0.10723514673252765 1107 3 0.11468224477378897 1107 4 0.06823562442122011 1107 5 0.07225007061891375 1107 6 0.1128133482424758 1107 7 0.09577070920582997 1107 8 0.05704063442300367 1107 9 0.03740288224329191 1107 10-11 0.05774277009567186 1107 12-13 0.12423231814064906 1107 14-15 0.3119487437855142 1107 16-17 0.21607545602238787 1107 18-19 0.17237902078160516 1107 20-21 0.14007711749710694 1107 22-23 0.1760559979652001 1107 24-25 0.19233781571956854 1107 26-27 0.15989782429961963 1107 28-29 0.22895635704433914 1107 30-31 0.27857321231672216 1107 32-33 0.3865702466494412 1107 34-35 0.40145873796681286 1107 36-37 0.285216672525749 1107 38-39 0.30067573775291123 1107 40-41 0.1120502542781523 1107 42-43 0.0061880591203262725 1107 44-45 0.11442609549634852 1107 46-47 0.11800948111648779 1107 48-49 0.12247742123104643 1107 50-51 0.06325026007126411 1107 52-53 -0.051600192457677 1107 54-55 0.19277403737306997 1107 56-57 0.17571471226831648 1107 58-59 0.1898380193286755 1107 60-61 0.34571678249394466 1107 62-63 0.4428261772406543 1107 64-65 0.3503107594085648 1107 66-67 0.10846232336836437 1107 68-69 0.3227541475367133 1107 70-71 0.20619199825733858 1107 72-73 0.21578700548131735 1107 74-75 0.17766715324050963 1107 76-77 0.10798839514727376 1107 78-79 0.21365507904287284 1107 80-81 0.2823921875917925 1107 82-83 0.20720744721332807 1107 84-85 0.20095977435646262 1107 86-87 0.327311320263739 1107 88-89 0.3426893085463085 1107 90-91 0.5504890783534186 1107 92-93 0.46874350150879707 1107 94-95 0.3432481142313968 1107 96-97 0.28805714871358035 1107 98-99 0.34436075059779725 1107 100-101 0.5999049656576325 1108 1 0.03952900293570849 1108 2 -0.031071098569441347 1108 3 -0.019315603343734722 1108 4 -0.0038440162859956217 1108 5 -0.028952414784285452 1108 6 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-0.2771474151477342 2316 86-87 -0.36749022029947653 2316 88-89 -0.26526273678636514 2316 90-91 -0.29037343342291067 2316 92-93 -0.48711331648759426 2316 94-95 -0.5422054460721135 2316 96-97 -0.5755091798129932 2316 98-99 -0.6112941875098059 2316 100-101 -0.5638609415361948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 14.0 5 51.0 6 89.0 7 166.0 8 445.0 9 773.0 10 1385.0 11 2354.0 12 3651.0 13 5719.0 14 7620.0 15 10466.0 16 15315.0 17 23528.0 18 23526.0 19 13169.0 20 8995.0 21 8269.0 22 8209.0 23 8565.0 24 9549.0 25 10948.0 26 12703.0 27 14881.0 28 18255.0 29 21893.0 30 26901.0 31 32846.0 32 41506.0 33 54188.0 34 78243.0 35 141180.0 36 192641.0 37 316923.0 38 325092.0 39 41708.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.14221119940612 19.91456192741813 15.713924178772082 19.22930269440367 2 15.450066146494235 21.85588163827317 40.842557844434246 21.851494370798346 3 18.8975915608454 28.495105168847207 30.85447790154995 21.752825368757446 4 11.870695618430599 18.83389853385298 37.70025813635674 31.59514771135969 5 11.909575954227837 39.624733596351405 33.24292368471845 15.222766764702309 6 27.833240539218167 38.24386293465607 17.917835028934892 16.00506149719087 7 25.657336640178162 37.02512189772401 20.180391759882575 17.137149702215247 8 26.542175911241284 34.30437922214649 19.3561753848438 19.797269481768424 9 27.790086605708165 16.904951028261415 20.511441361126163 34.79352100490426 10-11 24.2824652865651 28.800290192505614 25.55425081405747 21.362993706871826 12-13 26.676789661048407 26.920112702670785 27.08332911541901 19.319768520861803 14-15 23.326146007322297 26.873985591604665 28.083784650166187 21.71608375090685 16-17 19.497223764106838 30.69823471551807 28.535149626545238 21.269391893829855 18-19 19.419581245465743 27.334683065917563 33.919488451350574 19.326247237266116 20-21 21.21087884462891 25.8520693087682 34.250814057464865 18.686237789138026 22-23 20.345430311619513 26.534392873411957 33.97344401140524 19.146732803563292 24-25 19.683441380401835 26.74604478888724 34.52216041944152 19.048353411269403 26-27 19.548202753903073 26.885047935787714 34.43971862911005 19.127030681199166 28-29 19.186556388978214 27.166616782783077 34.552625922500354 19.094200905738358 30-31 19.41576825091529 27.14892611901267 34.42526699397682 19.01003863609522 32-33 19.100162474882193 27.259877139969664 34.12127746087021 19.51868292427793 34-35 19.57706653994705 27.24038566682372 34.02276498056681 19.15978281266242 36-37 19.663422212743672 27.027592481109636 34.003697084715746 19.305288221430946 38-39 19.54285922566997 27.16375084120085 33.98829439903666 19.305095534092512 40-41 19.562385422657087 27.17626078950708 34.015566319188515 19.24578746864732 42-43 19.3281780186516 27.36952402485755 33.65248364568376 19.649814310807095 44-45 19.440617254580395 27.64977922654208 33.273706783827606 19.635896735049915 46-47 19.697700291103672 27.87924208440623 32.437762965957376 19.985294658532727 48-49 19.710516103996895 28.055710212414166 32.07794705673938 20.155826626849553 50-51 20.06866764522279 28.42695415970711 31.645256533549293 19.859121661520813 52-53 20.039985827807865 28.650402389026674 31.38819996288235 19.921411820283105 54-55 19.635808405459667 28.628671694420543 31.3721718884446 20.363348011675185 56-57 19.864992998262217 28.91724452092929 30.958276391489935 20.259486089318553 58-59 19.940746739552225 28.534696563243404 31.1205479914292 20.40400870577517 60-61 19.85061834624015 28.60066474329773 31.346560712658807 20.202156197803312 62-63 20.036139089942807 28.53186212481652 31.319836007491013 20.11216277774966 64-65 19.76696866933239 28.58031752459044 31.386276593949823 20.266437212127347 66-67 19.621062576976936 28.76104671761907 31.153211519967606 20.464679185436385 68-69 19.898284049485294 28.823939691187622 30.994683060535227 20.283093198791853 70-71 19.763493107927992 28.858666126773635 30.771034738742387 20.606806026555986 72-73 19.837424710229286 28.917278264243894 30.53611378245685 20.70918324306997 74-75 19.75316765365862 29.108265424912688 30.338546675439925 20.80002024598876 76-77 19.762109632029155 28.880160618177523 30.774004150427697 20.583725599365625 78-79 19.740513910681447 28.949705589580066 30.65516019638609 20.6546203033524 80-81 19.664220276357746 29.344738573670092 30.339558974878102 20.65148217509406 82-83 19.72765770781664 29.31693408243492 30.172124647804154 20.78328356194429 84-85 19.75067766772297 28.963584281010828 30.223288688643358 21.062449362622843 86-87 19.80115699311365 29.006891748528112 30.203567080495514 20.98838417786272 88-89 19.855902403437504 28.880628517111422 30.13537494090696 21.128094138544114 90-91 19.631718218647524 29.020369516788335 30.318375351690168 21.02953691287397 92-93 19.692037537501562 28.987836518653516 30.292659833179407 21.02746611066551 94-95 19.455416645577095 28.91333029643502 30.281587960385348 21.34966509760254 96-97 19.590909274963657 28.809454073293228 30.098719507833177 21.500917143909934 98-99 19.601896374280845 28.755074150933847 29.99196909112382 21.651060383661488 100-101 19.708120328659884 28.721769499417928 30.22662010089251 21.343490071029677 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10523.0 1 6328.0 2 3500.5 3 4414.5 4 3002.5 5 2512.0 6 4936.5 7 10499.5 8 15441.5 9 16227.5 10 11921.5 11 7034.0 12 5961.0 13 5963.5 14 5822.0 15 5577.5 16 5286.0 17 5240.0 18 5406.0 19 5558.5 20 5813.5 21 6280.0 22 6897.5 23 7793.0 24 8878.0 25 10294.0 26 12209.0 27 14365.0 28 16136.0 29 18316.0 30 20915.5 31 22838.0 32 25525.5 33 28466.0 34 30897.0 35 33246.5 36 36419.5 37 39300.5 38 40608.5 39 41495.0 40 41995.0 41 43020.0 42 44209.0 43 45795.5 44 47883.5 45 49517.0 46 49227.0 47 49438.0 48 56176.0 49 56095.5 50 49763.0 51 46254.5 52 41815.5 53 39306.0 54 37651.5 55 34722.0 56 31049.0 57 27633.0 58 24974.5 59 22113.0 60 18606.5 61 15749.5 62 12846.0 63 9047.0 64 6081.0 65 4596.5 66 3338.0 67 2547.5 68 1912.0 69 1300.5 70 954.0 71 751.0 72 641.0 73 665.0 74 510.0 75 361.5 76 283.5 77 150.5 78 93.0 79 52.5 80 22.5 81 11.5 82 9.5 83 5.5 84 4.0 85 2.5 86 1.0 87 1.5 88 2.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014509625280491304 3 0.00742352921327462 4 0.0 5 6.748662921158745E-5 6 0.0 7 0.0 8 3.374331460579373E-4 9 1.349732584231749E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.6871657302896864E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006107539943648665 26-27 0.02119080157243846 28-29 3.3743314605793726E-5 30-31 0.0 32-33 5.061497190869059E-4 34-35 0.019503635842148773 36-37 0.004521604157176359 38-39 0.017681496853435914 40-41 0.016399250898415752 42-43 0.008941978370535337 44-45 0.0035430480336083414 46-47 3.3743314605793726E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.0122994381738119E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0025982352246461168 86-87 2.0245988763476237E-4 88-89 1.0122994381738119E-4 90-91 1.349732584231749E-4 92-93 2.362032022405561E-4 94-95 0.0 96-97 6.748662921158745E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1481775.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.22686174430683 #Duplication Level Percentage of deduplicated Percentage of total 1 73.715066307012 37.76191510181714 2 14.500626983463205 14.856432273752693 3 5.111563634050484 7.855480907361921 4 2.3653895287959066 4.84685929452236 5 1.2534133932942904 3.2104217303374547 6 0.7214492918107738 2.2174549876271143 7 0.4673924406222164 1.676013355526189 8 0.327285450472914 1.3412645217839314 9 0.23796301051512625 1.0971088415925663 >10 1.1801008355938043 10.898965656762025 >50 0.07584111423621957 2.661127982941468 >100 0.038649632627560014 3.6697284712497518 >500 0.0035494048162466388 1.2461064410049583 >1k 0.0011831349387488796 1.8590582798520796 >5k 1.314594376387644E-4 0.6362523591988136 >10k+ 3.943783129162932E-4 4.165809794669624 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25663 1.7319093654569688 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21584 1.4566314049029037 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14613 0.9861821126689275 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9448 0.6376136727910783 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4921 0.33210170235022185 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4525 0.3053769971824332 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4076 0.27507550066643044 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3330 0.2247304752745862 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2605 0.17580266909618533 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2361 0.159335931568558 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2318 0.1564340065124597 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1995 0.13463582527711696 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.3743314605793726E-5 0.0 12-13 0.0 0.0 0.0 6.748662921158745E-5 0.0 14-15 0.0 0.0 0.0 1.0122994381738117E-4 0.0 16-17 0.0 0.0 0.0 2.699465168463498E-4 0.0 18-19 0.0 0.0 0.0 5.061497190869059E-4 0.0 20-21 0.0 0.0 0.0 7.42352921327462E-4 0.0 22-23 0.0 0.0 0.0 0.0014509625280491303 0.0 24-25 0.0 0.0 0.0 0.0036780212920315162 0.0 26-27 0.0 0.0 0.0 0.00742352921327462 0.0 28-29 0.0 0.0 0.0 0.01673668404447369 0.0 30-31 0.0 0.0 0.0 0.03627406320122826 0.0 32-33 0.0 0.0 0.0 0.07160331359349428 0.0 34-35 0.0 0.0 0.0 0.11462603971588128 0.0 36-37 0.0 0.0 0.0 0.17775978134332138 0.0 38-39 0.0 0.0 0.0 0.2716336825766395 0.0 40-41 0.0 0.0 0.0 0.374280845607464 0.0 42-43 0.0 0.0 0.0 0.47358742049231495 0.0 44-45 0.0 0.0 0.0 0.5892595029609758 0.0 46-47 0.0 0.0 0.0 0.7278770393615765 0.0 48-49 0.0 0.0 0.0 0.8772249498068195 0.0 50-51 0.0 0.0 0.0 1.0351436621619343 0.0 52-53 0.0 0.0 0.0 1.1792613588432792 0.0 54-55 0.0 0.0 0.0 1.3330296435018814 0.0 56-57 0.0 0.0 0.0 1.5029272325420526 0.0 58-59 0.0 0.0 0.0 1.6907087783232946 0.0 60-61 0.0 0.0 0.0 1.910040323260954 0.0 62-63 0.0 0.0 0.0 2.119518820333721 0.0 64-65 0.0 0.0 0.0 2.3224848576875705 0.0 66-67 0.0 0.0 0.0 2.5451907340858093 0.0 68-69 0.0 0.0 0.0 2.7919555937979785 0.0 70-71 0.0 0.0 0.0 3.065647618565572 0.0 72-73 0.0 0.0 0.0 3.3352904455804695 0.0 74-75 0.0 0.0 0.0 3.6020650908538743 0.0 76-77 0.0 0.0 0.0 3.876668185115824 0.0 78-79 0.0 0.0 0.0 4.181167856118506 0.0 80-81 0.0 0.0 0.0 4.509996456951967 0.0 82-83 0.0 0.0 0.0 4.851512544077205 0.0 84-85 0.0 0.0 0.0 5.191037775640702 0.0 86-87 0.0 0.0 0.0 5.534241028496229 0.0 88-89 0.0 0.0 0.0 5.897184120396146 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11255 0.0 40.55881 1 GTATCAA 17990 0.0 34.74818 1 TATCAAC 22320 0.0 27.985876 2 ATCAACG 22575 0.0 27.625809 3 TCAACGC 22920 0.0 27.250504 4 CAACGCA 23055 0.0 27.090935 5 AACGCAG 23655 0.0 26.4841 6 TAAGGTG 615 0.0 26.258335 5 GTAAGGT 515 0.0 24.901176 4 GAGTACT 14390 0.0 24.490997 12-13 ACGCAGA 26295 0.0 23.698673 7 CGCAGAG 26345 0.0 23.671724 8 GTACTTT 15200 0.0 23.029646 14-15 GTACATG 14260 0.0 22.851353 1 AGTACTT 14480 0.0 22.7643 12-13 GCAGAGT 27305 0.0 22.61333 9 TACATGG 14460 0.0 22.206787 2 ACATGGG 14285 0.0 21.38005 3 ACTTTTT 16645 0.0 21.173054 16-17 CGGTATA 140 4.8516155E-4 20.357786 2 >>END_MODULE