FastQCFastQC Report
Thu 26 May 2016
SRR938192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499263
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259971.7339852981098045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207031.3808784716223905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140970.940261982053849No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98800.6589904506414151No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.31141967753489547No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41950.2798041437693053No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40270.2685986381308683No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36660.2445201408958935No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.17748720538024348No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25000.16674859581007467No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23630.15761077275968258No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19740.13166469125163496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118250.041.7251
GTATCAA188900.034.7167971
TATCAAC227000.028.7854862
ATCAACG232100.028.1734643
TCAACGC234000.027.9447064
CAACGCA236600.027.637625
AACGCAG241750.027.0246646
TATCACG1257.3245374E-626.631812
GAGTACT145400.025.43373112-13
ACGCAGA265850.024.4999897
CGCAGAG266300.024.4187988
GTACATG148500.023.8286861
GTACTTT155300.023.70447214-15
GCAGAGT272050.023.5008169
TACATGG148250.023.4495942
AGTACTT146350.022.57287612-13
ACATGGG148750.022.5395263
ACTTTTT171450.021.8217816-17
CATGGGG35750.021.151144
CAGAGTA270000.020.6683810-11
Produced by FastQC (version 0.11.5)