##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938192_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1499263 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06722436290364 33.0 31.0 34.0 30.0 34.0 2 32.151786577805225 33.0 31.0 34.0 30.0 34.0 3 32.129838460630324 34.0 31.0 34.0 30.0 34.0 4 35.68107396767612 37.0 35.0 37.0 35.0 37.0 5 35.71647202658907 37.0 35.0 37.0 35.0 37.0 6 35.78338423612135 37.0 36.0 37.0 35.0 37.0 7 35.73683403112062 37.0 36.0 37.0 35.0 37.0 8 35.73720954895839 37.0 36.0 37.0 35.0 37.0 9 37.36321112439912 39.0 37.0 39.0 35.0 39.0 10-11 37.28792146541333 39.0 37.0 39.0 34.0 39.0 12-13 37.1383479749717 39.0 37.0 39.0 34.0 39.0 14-15 38.23200665927192 40.0 38.0 41.0 33.5 41.0 16-17 38.089263524811855 40.0 38.0 41.0 33.0 41.0 18-19 38.36184011744437 40.0 38.0 41.0 34.0 41.0 20-21 38.416335893035445 40.0 38.0 41.0 34.0 41.0 22-23 38.28757829680316 40.0 38.0 41.0 34.0 41.0 24-25 38.16413597881092 40.0 38.0 41.0 34.0 41.0 26-27 37.97250182256215 40.0 38.0 41.0 33.0 41.0 28-29 37.76952109136289 40.0 37.5 41.0 33.0 41.0 30-31 37.44575834926894 40.0 36.5 41.0 33.0 41.0 32-33 37.04040952121142 39.0 35.5 41.0 32.0 41.0 34-35 36.76285248151925 39.0 35.0 41.0 31.0 41.0 36-37 36.47819595361187 39.0 35.0 41.0 31.0 41.0 38-39 36.1712924950459 39.0 35.0 40.0 30.0 41.0 40-41 35.95405409191049 39.0 35.0 40.0 30.0 41.0 42-43 35.64354052624523 38.5 35.0 40.0 28.5 41.0 44-45 35.158893069461456 38.0 35.0 40.0 26.0 41.0 46-47 34.558147903336504 38.0 34.5 40.0 23.5 41.0 48-49 33.98149957679206 38.0 34.0 40.0 19.0 41.0 50-51 33.201729783233496 37.5 33.0 39.5 15.5 40.5 52-53 33.26154717351125 38.0 33.0 39.5 14.5 40.5 54-55 33.36340255178711 38.0 33.0 40.0 11.0 41.0 56-57 33.08213535583817 38.0 33.0 40.0 8.0 41.0 58-59 32.727375717269084 37.0 33.0 40.0 4.5 41.0 60-61 32.27291409179043 36.5 32.0 40.0 2.0 41.0 62-63 31.782551827131066 36.0 31.0 39.0 2.0 41.0 64-65 31.298748785236477 35.0 31.0 39.0 2.0 40.5 66-67 30.797311412340598 35.0 30.5 39.0 2.0 40.0 68-69 30.188981519586623 35.0 29.0 38.0 2.0 40.0 70-71 29.6641606576031 35.0 29.0 37.5 2.0 40.0 72-73 29.010180001774206 34.0 28.0 37.0 2.0 39.0 74-75 28.392170686530648 34.0 26.5 36.0 2.0 39.0 76-77 27.69764277515019 34.0 26.0 35.5 2.0 38.5 78-79 26.99236591578662 33.0 24.5 35.0 2.0 37.0 80-81 26.315283575997007 33.0 22.5 35.0 2.0 37.0 82-83 25.621114507594733 32.0 19.5 35.0 2.0 36.0 84-85 24.92735964270445 32.0 16.5 35.0 2.0 36.0 86-87 24.205187482116216 31.0 8.5 34.5 2.0 35.0 88-89 23.365253461200602 31.0 2.0 34.0 2.0 35.0 90-91 22.507154848749018 30.0 2.0 34.0 2.0 35.0 92-93 21.614435892835345 29.0 2.0 34.0 2.0 35.0 94-95 20.592544470183014 29.0 2.0 34.0 2.0 35.0 96-97 19.39050753603604 26.5 2.0 34.0 2.0 35.0 98-99 17.9111916988547 24.0 2.0 33.0 2.0 35.0 100-101 14.011332568068443 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2890178580778695 1101 2 0.2944822546566357 1101 3 0.22911043291644262 1101 4 0.14440558364111666 1101 5 0.17062031128961763 1101 6 0.2372003330996293 1101 7 0.11055504606084554 1101 8 0.1674784347777134 1101 9 0.1421217405955204 1101 10-11 0.3475882009445499 1101 12-13 0.22192773360213636 1101 14-15 0.3560675364018593 1101 16-17 0.40504369535789664 1101 18-19 0.4981147614512622 1101 20-21 0.38516055315908915 1101 22-23 0.2960548996307679 1101 24-25 0.28067731059532264 1101 26-27 0.44316929008166284 1101 28-29 0.33488868885957146 1101 30-31 0.37202595482227707 1101 32-33 0.3984183966888466 1101 34-35 0.3151275409315133 1101 36-37 0.23045438580339805 1101 38-39 0.24301789897516812 1101 40-41 0.38714076817897336 1101 42-43 0.5303608188454447 1101 44-45 0.5335407741050417 1101 46-47 0.6553744967335007 1101 48-49 0.7249004938346033 1101 50-51 0.5275363356412726 1101 52-53 0.6897271842984054 1101 54-55 0.6688383784798262 1101 56-57 0.771793718429052 1101 58-59 0.746334320474098 1101 60-61 0.6146234527584511 1101 62-63 0.3814579915520824 1101 64-65 0.2592230073209514 1101 66-67 0.2797650334704933 1101 68-69 0.4532488520268494 1101 70-71 0.43707953241134234 1101 72-73 0.4070437511867162 1101 74-75 0.3314530399497926 1101 76-77 0.3470302581144935 1101 78-79 0.2308623169775963 1101 80-81 0.3447523854420318 1101 82-83 0.2435464411187951 1101 84-85 0.2784283799141285 1101 86-87 0.471921375336283 1101 88-89 0.3279865427556601 1101 90-91 0.25411778148834685 1101 92-93 0.34132152796069093 1101 94-95 0.0226553544093413 1101 96-97 -0.3560439463042542 1101 98-99 -0.43638443821022577 1101 100-101 -0.2164579322812905 1102 1 0.04547593405094119 1102 2 0.12068887403096085 1102 3 0.22290165911776683 1102 4 -0.024271354128146072 1102 5 0.05789604169932261 1102 6 0.07768473840607015 1102 7 0.11332304038626972 1102 8 0.03254595582271946 1102 9 0.07473613454398276 1102 10-11 0.09488524881624016 1102 12-13 0.17505860984179833 1102 14-15 0.29124991598244776 1102 16-17 0.0931406898099354 1102 18-19 0.09389593241239425 1102 20-21 0.1554157642517353 1102 22-23 0.20077296296707203 1102 24-25 0.002406318029343879 1102 26-27 0.24099829808263706 1102 28-29 0.26498895778464515 1102 30-31 0.15661659214761414 1102 32-33 0.1608811431011361 1102 34-35 0.11523664803601008 1102 36-37 0.26936725823690466 1102 38-39 0.0999178209245315 1102 40-41 0.1343354493966089 1102 42-43 0.5776775211147154 1102 44-45 0.3835953591547252 1102 46-47 0.20636474449860032 1102 48-49 -0.00715085201917276 1102 50-51 -0.027415659122596026 1102 52-53 -0.13580790982537394 1102 54-55 -0.26145339483189645 1102 56-57 -0.14902803697322753 1102 58-59 -0.14025287607176296 1102 60-61 -0.08042061529868505 1102 62-63 -0.21214072234477044 1102 64-65 -0.17372998402166218 1102 66-67 -0.13530056623116238 1102 68-69 -0.23883697721549169 1102 70-71 -0.30726085151715665 1102 72-73 -0.3558117682027877 1102 74-75 -0.2321324009292134 1102 76-77 -0.3994210837076082 1102 78-79 -0.3842749708793214 1102 80-81 -0.4190975078761703 1102 82-83 -0.4789183463270845 1102 84-85 -0.4201484401177318 1102 86-87 -0.3474817987229386 1102 88-89 -0.4836188120597633 1102 90-91 -0.49047143215306477 1102 92-93 -0.6399907306508617 1102 94-95 -0.7281347586727414 1102 96-97 -1.2134138099392722 1102 98-99 -1.3643951674954664 1102 100-101 -1.5490868780433438 1103 1 0.18633916633493897 1103 2 0.21658297375363844 1103 3 0.17754650023977803 1103 4 0.21320640389201628 1103 5 0.2534083977681192 1103 6 0.16269500799675995 1103 7 0.2326374388745478 1103 8 0.1965255938145205 1103 9 0.32078743145747524 1103 10-11 0.29917207692951564 1103 12-13 0.38102682309727953 1103 14-15 0.41532012349212977 1103 16-17 0.3150847019140315 1103 18-19 0.36881354982041614 1103 20-21 0.33926549089464686 1103 22-23 0.31518400590105244 1103 24-25 0.3035995382808281 1103 26-27 0.2557315380578373 1103 28-29 0.3314596975178432 1103 30-31 0.3825710539489009 1103 32-33 0.39481602433350815 1103 34-35 0.25071604520135793 1103 36-37 0.35730909850297365 1103 38-39 0.31970231498659274 1103 40-41 0.5669090305298994 1103 42-43 0.6902258990708461 1103 44-45 0.8630057244893266 1103 46-47 0.895587452936681 1103 48-49 0.7779847294578985 1103 50-51 0.8095562753561794 1103 52-53 0.667943761439787 1103 54-55 0.7062069278227483 1103 56-57 0.6163688690669105 1103 58-59 0.44333707480667783 1103 60-61 0.4585431093742116 1103 62-63 0.4393109536723756 1103 64-65 0.3401179767561864 1103 66-67 0.2969471218642674 1103 68-69 0.28032060541496406 1103 70-71 0.004978412052576431 1103 72-73 0.053289887545044934 1103 74-75 0.17582965585185661 1103 76-77 0.14353754400990582 1103 78-79 -0.12096219628290683 1103 80-81 -0.10156358244026009 1103 82-83 -0.47186075272596995 1103 84-85 -0.21753654381918608 1103 86-87 -0.5143836207319517 1103 88-89 -0.46304501738000425 1103 90-91 -0.5676869621443217 1103 92-93 -0.812592600825166 1103 94-95 -0.787407691598613 1103 96-97 -0.661114577357246 1103 98-99 -0.9583556156379984 1103 100-101 -1.2356792688662424 1104 1 0.3001515024503476 1104 2 0.23774251039102268 1104 3 0.31571943656660295 1104 4 0.24099142248803673 1104 5 0.3746314226106051 1104 6 0.2134327985112634 1104 7 0.18540935736443487 1104 8 0.3089588872211806 1104 9 0.28014378579522514 1104 10-11 0.28347487142336547 1104 12-13 0.2838802740890216 1104 14-15 0.2783764382230416 1104 16-17 0.27779697560736594 1104 18-19 0.4562526936305318 1104 20-21 0.26835860106507425 1104 22-23 0.427711918589047 1104 24-25 0.489105303660196 1104 26-27 0.4626058020930728 1104 28-29 0.3983520644871277 1104 30-31 0.3255953207294269 1104 32-33 0.30894169686337136 1104 34-35 0.39185816122965633 1104 36-37 0.4244245537496525 1104 38-39 0.3058130040868221 1104 40-41 0.33389692299335394 1104 42-43 0.35721693662591036 1104 44-45 0.4312896612271402 1104 46-47 0.2803190460215319 1104 48-49 0.3109980630471014 1104 50-51 0.26939931929312166 1104 52-53 0.3050417694240508 1104 54-55 0.1756395692892383 1104 56-57 0.22721735614458538 1104 58-59 0.2783079880399981 1104 60-61 0.22789888266961356 1104 62-63 0.4065148446304505 1104 64-65 0.06573565409004445 1104 66-67 0.1613251746687574 1104 68-69 0.22432198285847704 1104 70-71 0.08238844510802323 1104 72-73 0.12607077221133522 1104 74-75 0.09946443288884765 1104 76-77 -0.13303381571981276 1104 78-79 -0.11048024564378878 1104 80-81 -0.2596142973089215 1104 82-83 -0.42933671543051233 1104 84-85 -0.3679340247607321 1104 86-87 -0.20793469035628576 1104 88-89 -0.22938283540074167 1104 90-91 0.03290879283492387 1104 92-93 -0.32823916502581696 1104 94-95 -0.6386413937605688 1104 96-97 -1.0149552406197486 1104 98-99 -1.2492400740345637 1104 100-101 -1.3033256582211212 1105 1 0.03568648249478201 1105 2 0.1202313764927041 1105 3 0.21721032335772605 1105 4 0.20739167984947926 1105 5 0.13445689938273375 1105 6 0.18573879724456788 1105 7 0.19115072922155463 1105 8 0.12511535493538872 1105 9 0.10571153921079457 1105 10-11 0.18816191588859255 1105 12-13 0.1412007494034455 1105 14-15 0.12258379514113926 1105 16-17 0.191187059149307 1105 18-19 0.1681795366300065 1105 20-21 0.2271847916130696 1105 22-23 0.20303362039158657 1105 24-25 0.2011447422905448 1105 26-27 0.09919812548381657 1105 28-29 0.07643939647334008 1105 30-31 0.08917415275082163 1105 32-33 0.06432869407863961 1105 34-35 0.12989537810693008 1105 36-37 0.1013439573949384 1105 38-39 -0.013869616978617216 1105 40-41 0.01495597538048088 1105 42-43 0.14478416727605037 1105 44-45 0.11432818501799602 1105 46-47 0.15255844242852135 1105 48-49 0.0999564026636719 1105 50-51 0.10352434065612925 1105 52-53 0.017279507056123578 1105 54-55 0.042505037616685115 1105 56-57 0.02150513851331226 1105 58-59 -0.08089243218298492 1105 60-61 0.019705791215820057 1105 62-63 -0.018722159119384685 1105 64-65 -0.29472618906022063 1105 66-67 -0.2749067421713285 1105 68-69 -0.3800358199182128 1105 70-71 -0.3609794711690846 1105 72-73 -0.5391482363439231 1105 74-75 -0.4503686843010861 1105 76-77 -0.37139424237682306 1105 78-79 -0.40318615993036033 1105 80-81 -0.2275092114044881 1105 82-83 -0.5504594547213593 1105 84-85 -0.7873997484389896 1105 86-87 -0.5804714096010919 1105 88-89 -0.5337831128685444 1105 90-91 -0.45441791545191634 1105 92-93 -0.8168019119570822 1105 94-95 -0.7071186383846317 1105 96-97 -0.8429943173355738 1105 98-99 -0.7994092188104247 1105 100-101 -0.7583062555811555 1106 1 0.17532276763859755 1106 2 0.2145571009544156 1106 3 0.19225754153267616 1106 4 0.21531354051538898 1106 5 0.23236850695922584 1106 6 0.18250907407797712 1106 7 0.19473931051776106 1106 8 -0.003420611823017339 1106 9 0.2465323260004979 1106 10-11 0.24195690806386239 1106 12-13 0.40646840900087255 1106 14-15 0.4654767852657926 1106 16-17 -0.13842952927010543 1106 18-19 0.1632755918887625 1106 20-21 0.348084904936691 1106 22-23 0.363309644351439 1106 24-25 0.39807016455593924 1106 26-27 0.29760215117050137 1106 28-29 0.25068660385556285 1106 30-31 0.30599191757733735 1106 32-33 0.34927823808597935 1106 34-35 0.3827743376881756 1106 36-37 0.27410581590230265 1106 38-39 0.30604026376176563 1106 40-41 0.3322445728657826 1106 42-43 0.2938910692241379 1106 44-45 0.48882902145423657 1106 46-47 0.5787996058843561 1106 48-49 0.4663599966413372 1106 50-51 0.48950728814951816 1106 52-53 0.48260787573563135 1106 54-55 0.3651495252320345 1106 56-57 0.025994912405707282 1106 58-59 0.09890493439195325 1106 60-61 0.4173678170104367 1106 62-63 0.3949926970675435 1106 64-65 0.38967832229912247 1106 66-67 0.3320505186348903 1106 68-69 0.14840166321815218 1106 70-71 -0.07873620313174001 1106 72-73 -0.14036781434834822 1106 74-75 -0.02704290587574576 1106 76-77 -0.4307029682955559 1106 78-79 -0.5911549689530808 1106 80-81 -0.3939182017449987 1106 82-83 -0.29501722278568465 1106 84-85 -0.3078587090995022 1106 86-87 -0.4986301705961793 1106 88-89 -0.8351160144928471 1106 90-91 -1.0704032913085868 1106 92-93 -0.8518175614093515 1106 94-95 -1.1564144130325182 1106 96-97 -1.2263370943039007 1106 98-99 -1.1514962621432225 1106 100-101 -1.3836057537194097 1107 1 0.05113475589151051 1107 2 0.014107833202508857 1107 3 -0.009671781453882033 1107 4 0.04357170415777034 1107 5 0.01367867674743195 1107 6 0.030058522703917845 1107 7 0.10902446912289321 1107 8 0.15112700861380546 1107 9 0.20786175012091945 1107 10-11 0.17307647941185422 1107 12-13 0.1860021721531382 1107 14-15 0.02857775381705352 1107 16-17 0.1642495457439992 1107 18-19 0.09238415520439247 1107 20-21 0.12334819334325431 1107 22-23 0.2599802494042436 1107 24-25 0.2553790312731721 1107 26-27 0.20292748131422655 1107 28-29 0.25532514752789126 1107 30-31 0.03957273715062826 1107 32-33 0.11687212726660334 1107 34-35 0.1350537394424549 1107 36-37 0.10886790820639902 1107 38-39 7.725507485574212E-4 1107 40-41 -0.030961662426051362 1107 42-43 -0.02174189263672588 1107 44-45 -0.0924522182135803 1107 46-47 0.023372412543622545 1107 48-49 0.03433654136389919 1107 50-51 -0.07799689334687798 1107 52-53 -0.02430374882433739 1107 54-55 0.17393869029417175 1107 56-57 0.022335373731728225 1107 58-59 0.09600754636853281 1107 60-61 0.11301833143173923 1107 62-63 0.23446383501802615 1107 64-65 0.013345591879225793 1107 66-67 0.0469573101629841 1107 68-69 0.11066268583713068 1107 70-71 0.19722189438464355 1107 72-73 0.21555702568510426 1107 74-75 0.06092483825557338 1107 76-77 0.02878270764819746 1107 78-79 -0.12114748115721596 1107 80-81 -0.2706644627092736 1107 82-83 -0.237845956472988 1107 84-85 -0.1341386845939887 1107 86-87 0.057899041532106565 1107 88-89 -0.15377399726119378 1107 90-91 -0.1597737816133531 1107 92-93 -0.15410887556614838 1107 94-95 -0.2250057835908983 1107 96-97 -0.19459759180472247 1107 98-99 -0.30830140441134546 1107 100-101 -0.750432873154617 1108 1 -0.08286269805104496 1108 2 -0.017657188376688282 1108 3 0.014967793287432585 1108 4 0.08601850689102264 1108 5 0.08497587225078007 1108 6 0.16483584650045202 1108 7 0.1737077453270004 1108 8 -0.029283283591809095 1108 9 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#Quality Count 2 4933.0 3 1202.0 4 1107.0 5 1220.0 6 1345.0 7 1731.0 8 2343.0 9 2857.0 10 3568.0 11 5257.0 12 7187.0 13 9989.0 14 11720.0 15 15799.0 16 20925.0 17 29149.0 18 26877.0 19 17129.0 20 15840.0 21 16082.0 22 17083.0 23 18622.0 24 21218.0 25 23612.0 26 27053.0 27 30913.0 28 35842.0 29 41988.0 30 49555.0 31 59416.0 32 72547.0 33 91747.0 34 122781.0 35 177533.0 36 190512.0 37 205766.0 38 110176.0 39 6639.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.788449180428614 19.48080229685732 15.499311290165277 19.231437232548785 2 15.274792375016267 21.67241131935709 41.35449234064845 21.698303964978194 3 18.898711812780338 28.700290234558956 30.509029334063655 21.891968618597055 4 11.685532827119188 18.525834952817906 38.08473410400764 31.703898116055267 5 11.906076129709993 39.57543406177035 33.060999194090535 15.45749061442912 6 27.62635746984329 38.182377117652955 18.19313663247235 15.99812878003141 7 25.632093831485737 37.01636345537279 20.330956947804367 17.020585765337106 8 26.50806989346713 33.98599540922813 19.562975605100174 19.942959092204564 9 28.07506325724211 16.91534381287743 20.529938422271893 34.479654507608565 10-11 24.212964323007775 28.827919069124004 25.643856341285588 21.31526026658263 12-13 26.761058258173204 26.99188257251377 27.04652424206393 19.200534927249095 14-15 23.450095525416508 26.775335107034888 28.201687699178258 21.57288166837035 16-17 19.48778108709104 30.627276822551686 28.544980780269945 21.339961310087325 18-19 19.395089926736254 27.328511416988903 34.002257351418855 19.274141304855977 20-21 21.383984877066055 25.855415161068528 34.216639835547106 18.543960126318307 22-23 20.485474750762073 26.521214251561155 33.874338709639694 19.11897228803708 24-25 19.632435727383648 26.7502213185674 34.51854480827913 19.098798145769816 26-27 19.553893388488543 26.871966861453263 34.3761991866321 19.197940563426098 28-29 19.23607714024786 27.136892051402345 34.48838906038957 19.138641747960218 30-31 19.424630701050447 27.162950866515605 34.3341536267607 19.078264805673243 32-33 19.12923826295077 27.242649047486445 34.05999763599537 19.568115053567407 34-35 19.632896882804587 27.222035666862716 33.99634292153704 19.148724528795654 36-37 19.688748733326303 27.070302226007964 33.9015619304975 19.339387110168236 38-39 19.57519902223103 27.19898752997554 33.86295819156784 19.362855256225586 40-41 19.641810787254123 27.241860974187098 33.858694418972796 19.257633819585983 42-43 19.399257581359713 27.432982768999953 33.48502281849453 19.68273683114581 44-45 19.464727175671843 27.75138038600764 33.073214672961115 19.7106777653594 46-47 19.730795218512 27.96996971946713 32.32913187464026 19.97010318738061 48-49 19.775054249620318 28.113681313841727 31.93100622702899 20.180258209508963 50-51 20.083998788100427 28.50226296886032 31.52704017553858 19.88669806750067 52-53 20.030242762719407 28.718882412162223 31.297856853591803 19.953017971526567 54-55 19.655710420912563 28.615134006804112 31.376184012609894 20.352971559673435 56-57 19.937406429884792 28.910630365687357 30.80561700901209 20.346346195415766 58-59 19.977798071895673 28.45156733133425 31.11536494210782 20.455269654662256 60-61 20.001174329948014 28.483739867233993 31.32284932479364 20.19223647802435 62-63 20.167547659357798 28.379665251695197 31.359380286540063 20.09340680240694 64-65 19.846985193955874 28.471650190484482 31.382314485728596 20.29905012983105 66-67 19.737998812967678 28.53721786713845 31.292814317518136 20.431969002375734 68-69 19.98980187063593 28.621337049510316 31.118210201599933 20.270650878253825 70-71 19.85943746959417 28.660828747016144 30.849614842783918 20.630118940605772 72-73 19.897198569254996 28.70438832981875 30.72001041027147 20.678402690654778 74-75 19.832706703576044 28.8951108866091 30.594734975482535 20.677447434332326 76-77 19.914853674141014 28.65481080410341 30.733267109154482 20.697068412601098 78-79 19.930851019472126 28.714272609765253 30.64654130008756 20.70833507067506 80-81 19.8310672531933 29.12116743129289 30.31782720503736 20.729938110476454 82-83 19.960471737292167 29.019048619763133 30.134679629268263 20.885800013676445 84-85 20.065391939686243 28.74816998301824 30.069051139739077 21.117386937556436 86-87 20.11273499104518 28.69301664172082 30.122085096971908 21.07216327026209 88-89 20.314445407374457 28.42462610636376 30.153561938183454 21.107366548078332 90-91 20.082615078036294 28.681347040416487 30.133504176658587 21.102533704888632 92-93 20.209139009588515 28.54569588233586 30.159345755558142 21.085819352517483 94-95 20.042585429508765 28.50747414336872 30.039915496624204 21.41002493049831 96-97 20.240360116359042 28.23949379744356 29.848891422141254 21.671254664056143 98-99 20.283241426577924 28.18589879200955 29.63373884422389 21.897120937188635 100-101 21.120627188451056 27.337915208812376 29.870157191142475 21.671300411594093 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10756.0 1 6523.0 2 3556.5 3 4349.0 4 2998.5 5 2565.0 6 4618.0 7 9873.5 8 15198.0 9 15920.5 10 11391.0 11 6885.5 12 5919.0 13 5937.5 14 5879.5 15 5621.0 16 5414.5 17 5388.5 18 5610.5 19 5802.5 20 5929.0 21 6453.5 22 7221.0 23 8231.5 24 9411.5 25 10862.0 26 12866.0 27 15085.5 28 16725.5 29 18636.0 30 21203.0 31 23261.0 32 26037.5 33 28912.5 34 31470.5 35 33857.5 36 36739.5 37 39543.5 38 41034.5 39 41723.0 40 41951.0 41 42907.0 42 44168.5 43 45633.5 44 47286.0 45 48811.5 46 49144.0 47 49873.0 48 54771.0 49 55200.5 50 50203.5 51 46151.5 52 42297.5 53 39871.0 54 37883.0 55 35068.0 56 31621.0 57 28368.5 58 25743.5 59 22827.0 60 19423.5 61 16616.5 62 13830.0 63 10260.5 64 7103.5 65 5307.5 66 4021.5 67 3023.0 68 2288.5 69 1699.0 70 1298.0 71 1009.0 72 802.5 73 724.0 74 596.5 75 458.0 76 342.5 77 236.5 78 156.5 79 86.5 80 51.5 81 35.0 82 22.5 83 15.0 84 10.5 85 6.5 86 5.5 87 5.0 88 4.5 89 3.5 90 2.0 91 1.0 92 0.5 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15080743005063155 2 0.051225168632854944 3 0.14660536543621766 4 0.12492804798090795 5 0.18789231775879217 6 0.19429546383789903 7 0.16354702277052124 8 0.06936741585699108 9 0.12045918561319795 10-11 0.09944886254112854 12-13 0.14920664353085483 14-15 0.1345661168187303 16-17 0.13163134153247297 18-19 0.12893001428034975 20-21 0.1290300634378358 22-23 0.1901934483809712 24-25 0.20786879953683912 26-27 0.14370393986912236 28-29 0.12409430500185759 30-31 0.09504669961174257 32-33 0.1203257867365499 34-35 0.08977744398414421 36-37 0.08304080071341718 38-39 0.04999122902386039 40-41 0.057695014150285835 42-43 0.06116338494313539 44-45 0.03921926973452956 46-47 0.051725414420285165 48-49 0.04235414333575897 50-51 0.05262585683765957 52-53 0.02614617982301971 54-55 0.05049147481129061 56-57 0.02581268263139956 58-59 0.04965773183224024 60-61 0.03575089894168001 62-63 0.05095837087955883 64-65 0.03898582170039546 66-67 0.03785193124888695 68-69 0.06329776696950436 70-71 0.05229235964603942 72-73 0.04955768267475419 74-75 0.04252089193156905 76-77 0.02094362363374538 78-79 0.02171066717447172 80-81 0.025512535158941425 82-83 0.022377661557712022 84-85 0.020109880654695007 86-87 0.02331145369424844 88-89 0.06286422062039815 90-91 0.10635225440766563 92-93 0.07793829368162891 94-95 0.0732359832797848 96-97 0.06466510545514696 98-99 0.05496033717900062 100-101 0.1276627249521932 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1499263.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.20386047783241 #Duplication Level Percentage of deduplicated Percentage of total 1 75.65132466776969 40.249425225872194 2 13.48963562541349 14.354013834225936 3 4.756891244048076 7.592549341696693 4 2.187815021601498 4.656008206423679 5 1.1013428889579109 2.9297846701184778 6 0.6666060634904435 2.1279609597373588 7 0.44305854864909205 1.650069764408605 8 0.28741855728696425 1.223342145650842 9 0.21513048004935956 1.0301194840567711 >10 1.0897476285838605 10.381314943611892 >50 0.07164334766653106 2.6131241109704617 >100 0.03461954619816019 3.476483014011182 >500 0.0031357765032501398 1.1607740873599912 >1k 0.0011288795411700501 1.8384595453995858 >5k 1.2543106013000556E-4 0.6593335623270502 >10k+ 3.7629318039001676E-4 4.057237104129319 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25997 1.7339852981098045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20703 1.3808784716223905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14097 0.940261982053849 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9880 0.6589904506414151 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4669 0.31141967753489547 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4195 0.2798041437693053 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4027 0.2685986381308683 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3666 0.2445201408958935 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2661 0.17748720538024348 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2500 0.16674859581007467 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2363 0.15761077275968258 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1974 0.13166469125163496 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3339887664805974E-4 0.0 20-21 0.0 0.0 0.0 5.002457874302241E-4 0.0 22-23 0.0 0.0 0.0 0.0013339887664805975 0.0 24-25 0.0 0.0 0.0 0.003001474724581344 0.0 26-27 0.0 0.0 0.0 0.006069648887486718 0.0 28-29 0.0 0.0 0.0 0.013906832890560229 0.0 30-31 0.0 0.0 0.0 0.034350210736875386 0.0 32-33 0.0 0.0 0.0 0.06383136247609658 0.0 34-35 0.0 0.0 0.0 0.10655235272263772 0.0 36-37 0.0 0.0 0.0 0.17111740902029865 0.0 38-39 0.0 0.0 0.0 0.2641631254823203 0.0 40-41 0.0 0.0 0.0 0.37168262006065644 0.0 42-43 0.0 0.0 0.0 0.4726322199640757 0.0 44-45 0.0 0.0 0.0 0.5864214617448706 0.0 46-47 0.0 0.0 0.0 0.723255359466618 0.0 48-49 0.0 0.0 0.0 0.8662923049525 0.0 50-51 0.0 0.0 0.0 1.017933477982182 0.0 52-53 0.0 0.0 0.0 1.1618041664471144 0.0 54-55 0.0 0.0 0.0 1.3110441596971312 0.0 56-57 0.0 0.0 0.0 1.4882312176049166 0.0 58-59 0.0 0.0 0.0 1.68342712386019 0.0 60-61 0.0 0.0 0.0 1.895798135483901 0.0 62-63 0.0 0.0 0.0 2.101632602151857 0.0 64-65 0.0 0.0 0.0 2.304332195218584 0.0 66-67 0.0 0.0 0.0 2.520538424545927 0.0 68-69 0.0 0.0 0.0 2.765292013475954 0.0 70-71 0.0 0.0 0.0 3.0335238046960407 0.0 72-73 0.0 0.0 0.0 3.3028894863676355 0.0 74-75 0.0 0.0 0.0 3.5625170500439216 0.0 76-77 0.0 0.0 0.0 3.8316826334005443 0.0 78-79 0.0 0.0 0.0 4.135631973843148 0.0 80-81 0.0 0.0 0.0 4.456823119092514 0.0 82-83 0.0 0.0 0.0 4.787152087392272 0.0 84-85 0.0 0.0 0.0 5.123117158230411 0.0 86-87 0.0 0.0 0.0 5.458015038055365 0.0 88-89 0.0 6.669943832402987E-5 0.0 5.810388170721215 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11825 0.0 41.725 1 GTATCAA 18890 0.0 34.716797 1 TATCAAC 22700 0.0 28.785486 2 ATCAACG 23210 0.0 28.173464 3 TCAACGC 23400 0.0 27.944706 4 CAACGCA 23660 0.0 27.63762 5 AACGCAG 24175 0.0 27.024664 6 TATCACG 125 7.3245374E-6 26.63181 2 GAGTACT 14540 0.0 25.433731 12-13 ACGCAGA 26585 0.0 24.499989 7 CGCAGAG 26630 0.0 24.418798 8 GTACATG 14850 0.0 23.828686 1 GTACTTT 15530 0.0 23.704472 14-15 GCAGAGT 27205 0.0 23.500816 9 TACATGG 14825 0.0 23.449594 2 AGTACTT 14635 0.0 22.572876 12-13 ACATGGG 14875 0.0 22.539526 3 ACTTTTT 17145 0.0 21.82178 16-17 CATGGGG 3575 0.0 21.15114 4 CAGAGTA 27000 0.0 20.66838 10-11 >>END_MODULE