FastQCFastQC Report
Thu 26 May 2016
SRR938192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499263
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254091.694766028375275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214081.4279015756408315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147760.9855509006758655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93540.6239065460829754No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48450.32315877867992476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42290.28207192467232234No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40740.2717335117320977No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33780.22531070265857292No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25630.17095066042448856No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25410.1694832727813599No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23170.15454259859677721No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19390.12933021091029392No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15230.1015832445674975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122050.039.0024031
GCGCGAC600.0039554131.6599969
GTATCAA185150.030.995931
GTGTAGC9750.026.799061
TATCACG1457.88179E-726.2101172
TATCAAC226450.025.3000832
ATCAACG228300.025.0509433
TCAACGC230900.024.7647324
CAACGCA233550.024.5244085
AACGCAG238900.024.014966
GAGTACT144800.023.91057812-13
GTACATG147600.023.8502711
TACATGG146600.023.6232992
TAGCCCT10450.023.1784824
GTACTTT152400.022.60911414-15
ACATGGG148800.022.3140163
AGTACTT145500.021.91866512-13
ACGCAGA265750.021.5335647
CGCAGAG266650.021.4430738
AAGGCGC1353.8003802E-421.1080746
Produced by FastQC (version 0.11.5)