##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938192_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1499263 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.537942308987816 33.0 31.0 34.0 31.0 34.0 2 32.7200324426068 34.0 31.0 34.0 31.0 34.0 3 32.74384814405478 34.0 31.0 34.0 31.0 34.0 4 36.21749486247576 37.0 35.0 37.0 35.0 37.0 5 36.21974930349111 37.0 35.0 37.0 35.0 37.0 6 36.279930872702124 37.0 37.0 37.0 35.0 37.0 7 36.243390919405066 37.0 37.0 37.0 35.0 37.0 8 36.24480294651439 37.0 37.0 37.0 35.0 37.0 9 37.906215253761346 39.0 38.0 39.0 35.0 39.0 10-11 37.901535287671344 39.0 38.0 39.0 35.0 39.0 12-13 37.817961224948526 39.0 38.0 39.0 35.0 39.0 14-15 38.99408075834593 40.0 38.0 41.0 35.5 41.0 16-17 38.906140550390425 40.0 38.0 41.0 35.5 41.0 18-19 39.23054394058948 40.5 39.0 41.0 36.0 41.0 20-21 39.256670110581 41.0 39.0 41.0 36.0 41.0 22-23 39.13892425811882 40.5 39.0 41.0 35.0 41.0 24-25 39.03350779683085 40.5 38.5 41.0 35.0 41.0 26-27 38.8382031704911 40.0 38.0 41.0 35.0 41.0 28-29 38.694790373670266 40.0 38.0 41.0 35.0 41.0 30-31 38.35381684200837 40.0 37.5 41.0 34.5 41.0 32-33 38.01067357761781 40.0 36.5 41.0 34.0 41.0 34-35 37.79214687483116 40.0 36.0 41.0 33.5 41.0 36-37 37.716403326167594 40.0 36.0 41.0 33.0 41.0 38-39 37.54194827725355 40.0 35.5 41.0 33.0 41.0 40-41 37.3188736732648 40.0 35.0 41.0 33.0 41.0 42-43 37.012200661258234 40.0 35.0 41.0 32.5 41.0 44-45 36.59492063767331 40.0 35.0 41.0 31.5 41.0 46-47 35.97994247840439 39.0 35.0 41.0 29.5 41.0 48-49 35.41540110040734 39.0 35.0 41.0 27.0 41.0 50-51 34.999529101965436 39.0 35.0 40.5 25.0 41.0 52-53 34.511648056411715 38.0 35.0 40.0 23.0 41.0 54-55 34.248112239146835 38.0 34.0 40.0 23.0 41.0 56-57 33.94332615425046 38.0 34.0 40.0 21.5 41.0 58-59 33.57031154640647 37.5 33.5 40.0 19.5 41.0 60-61 33.1553973518989 37.0 33.0 40.0 18.0 41.0 62-63 32.74010430458165 36.0 33.0 39.5 14.0 41.0 64-65 32.302097097040345 36.0 32.0 39.0 9.5 40.5 66-67 31.806824419731562 35.0 31.0 39.0 7.5 40.0 68-69 31.3024092504117 35.0 31.0 38.0 3.5 40.0 70-71 30.78898999041529 35.0 30.5 37.5 2.0 40.0 72-73 30.269476402739212 35.0 29.5 37.0 2.0 39.0 74-75 29.728132755894062 34.0 29.0 36.0 2.0 39.0 76-77 28.35100279270548 32.5 27.0 35.0 2.0 37.0 78-79 29.051453280711925 34.0 29.0 35.0 2.0 37.5 80-81 28.763031569511153 34.0 29.0 35.0 2.0 37.0 82-83 28.25436864646163 34.0 27.5 35.0 2.0 36.5 84-85 27.71349923262296 33.5 26.5 35.0 2.0 36.0 86-87 27.126731267295998 33.0 26.0 35.0 2.0 36.0 88-89 26.46034451593883 33.0 24.5 35.0 2.0 35.0 90-91 25.97279163162167 32.0 23.5 35.0 2.0 35.0 92-93 25.24122185367077 32.0 19.5 34.0 2.0 35.0 94-95 24.513049078113713 31.5 14.5 34.0 2.0 35.0 96-97 23.566093140429665 31.0 2.0 34.0 2.0 35.0 98-99 22.273338300218175 31.0 2.0 34.0 2.0 35.0 100-101 18.050596526426652 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12436829940029526 1101 2 0.09559460453900215 1101 3 0.12725799697696516 1101 4 0.11002882878706544 1101 5 0.06377932730671887 1101 6 0.024680618484822503 1101 7 0.09081321795333963 1101 8 0.13597955763781755 1101 9 -0.022676368415936565 1101 10-11 0.09363158564568863 1101 12-13 0.19648888374310758 1101 14-15 0.08601196320385895 1101 16-17 0.14041370232611428 1101 18-19 0.23505807547510926 1101 20-21 0.28178061526964626 1101 22-23 0.22897526006961755 1101 24-25 0.2581836919408431 1101 26-27 0.250904994302779 1101 28-29 0.2291072530405387 1101 30-31 0.17715545238858965 1101 32-33 0.18909471159873448 1101 34-35 0.11499629543761358 1101 36-37 0.1607666416146145 1101 38-39 0.23371717713530415 1101 40-41 0.2143547493611493 1101 42-43 0.22530179565464437 1101 44-45 -0.01972676275554619 1101 46-47 -0.2297248861953065 1101 48-49 -0.06559074098659323 1101 50-51 -0.2770985879749901 1101 52-53 -0.4253792195773016 1101 54-55 -0.30551491049646273 1101 56-57 -0.07121230199394546 1101 58-59 0.07286496226853956 1101 60-61 -0.09260812898187254 1101 62-63 -0.1769163032660046 1101 64-65 -0.17133401376378998 1101 66-67 -0.3749603346748742 1101 68-69 -0.17718272238784039 1101 70-71 -0.11601372122954601 1101 72-73 -0.33008836472846426 1101 74-75 -0.43479357952168485 1101 76-77 -0.5031172244514153 1101 78-79 -0.5327274760027016 1101 80-81 -0.5596966594747173 1101 82-83 -0.49757414497454633 1101 84-85 -0.371695045175791 1101 86-87 -0.5394786116856274 1101 88-89 -0.7556000107140868 1101 90-91 -0.6242479135254548 1101 92-93 -0.6842397611575066 1101 94-95 -0.9082349994279006 1101 96-97 -0.8926113680076853 1101 98-99 -0.6975450715980891 1101 100-101 -1.1795724504098075 1102 1 0.09476827269047305 1102 2 0.1115373447273953 1102 3 0.06755839428556953 1102 4 0.09436137615447393 1102 5 0.15669801164638386 1102 6 0.029274203969798407 1102 7 0.07512938665610847 1102 8 0.08008484473376143 1102 9 0.1467413889543039 1102 10-11 0.09629091383066424 1102 12-13 0.18122743300917676 1102 14-15 0.09520763848045277 1102 16-17 0.06563848374968018 1102 18-19 0.17393289901074382 1102 20-21 0.18234259245163997 1102 22-23 0.15569207484771397 1102 24-25 0.1391684402543163 1102 26-27 0.11401369005081818 1102 28-29 0.022329446635097838 1102 30-31 0.06704704452707233 1102 32-33 0.10884240456932304 1102 34-35 0.22521084964501625 1102 36-37 0.3863342410882282 1102 38-39 0.29718576254153817 1102 40-41 0.26314742129460456 1102 42-43 0.3380477984730348 1102 44-45 0.3805889045081017 1102 46-47 0.2003158525832589 1102 48-49 0.32686474211786276 1102 50-51 0.326850812030429 1102 52-53 0.350271501619396 1102 54-55 0.260634421348513 1102 56-57 0.32778078809941036 1102 58-59 0.4200321777254672 1102 60-61 0.21606040527253612 1102 62-63 0.4265691395658493 1102 64-65 0.455495750805504 1102 66-67 0.3605888010040985 1102 68-69 0.40487092277847125 1102 70-71 0.3544262537992928 1102 72-73 0.3210142658740587 1102 74-75 0.3214682674499336 1102 76-77 0.3184258851388613 1102 78-79 0.06648691504799586 1102 80-81 0.2915847505519231 1102 82-83 0.25465414085330806 1102 84-85 0.20187728647201197 1102 86-87 -0.09055831215764343 1102 88-89 -0.04023442946992262 1102 90-91 -0.342587797274728 1102 92-93 -0.22556533688331726 1102 94-95 -0.3874703173724292 1102 96-97 -0.5260600029404792 1102 98-99 -0.5381718452972315 1102 100-101 -0.8272954065571909 1103 1 0.11647961300552367 1103 2 0.13607113581676344 1103 3 0.11652154989231178 1103 4 0.14016298796101267 1103 5 0.09417700166542886 1103 6 0.15773003416984466 1103 7 0.143297058184082 1103 8 0.10466556911390512 1103 9 0.19796848917506793 1103 10-11 0.16726431614811332 1103 12-13 0.10591152796227021 1103 14-15 0.18803875431357397 1103 16-17 0.19980433543931042 1103 18-19 0.21128211848078848 1103 20-21 0.22904943010410506 1103 22-23 0.2645174695126826 1103 24-25 0.22545001535902287 1103 26-27 0.32276798078026303 1103 28-29 0.2406833553275547 1103 30-31 0.25303135590129955 1103 32-33 0.19297736828695378 1103 34-35 0.1781860389475085 1103 36-37 0.09325285348202073 1103 38-39 0.20083347656760964 1103 40-41 0.1869635437013244 1103 42-43 0.23450386568620019 1103 44-45 0.1137646397621026 1103 46-47 -0.10220000646902605 1103 48-49 -0.15768540522333296 1103 50-51 -0.2755148018247482 1103 52-53 -0.2713288414629531 1103 54-55 -0.5090495474713563 1103 56-57 -0.3152096955892105 1103 58-59 -0.29971975636611603 1103 60-61 -0.44947536464169957 1103 62-63 -0.4921113074972183 1103 64-65 -0.5095371875646286 1103 66-67 -0.569894195357989 1103 68-69 -0.6318907505576092 1103 70-71 -0.7700021133856545 1103 72-73 -0.809391642557916 1103 74-75 -0.7504033207198297 1103 76-77 -0.5953834730073204 1103 78-79 -0.5514385513662496 1103 80-81 -0.7088127157543767 1103 82-83 -0.8362893766912372 1103 84-85 -0.9248203053221715 1103 86-87 -1.261666530352862 1103 88-89 -1.4324037272364265 1103 90-91 -1.1615919080443398 1103 92-93 -1.143189784903818 1103 94-95 -1.1968293435535777 1103 96-97 -1.1458419743381576 1103 98-99 -1.0120118156823246 1103 100-101 -1.5848324530569364 1104 1 0.14455035517784154 1104 2 0.09019864209494699 1104 3 0.1542269337027662 1104 4 0.13002886313072537 1104 5 0.11324851731706076 1104 6 0.10798747857501922 1104 7 0.10787000005479541 1104 8 0.10804041232575656 1104 9 0.16562308775209544 1104 10-11 0.08029880181103266 1104 12-13 0.22004663576694838 1104 14-15 0.17198732620210677 1104 16-17 0.15504381817302715 1104 18-19 0.14306055050869304 1104 20-21 0.08343248840184714 1104 22-23 0.15299705227096894 1104 24-25 0.11548375312980852 1104 26-27 0.22709109576427267 1104 28-29 0.0460474956327559 1104 30-31 0.059111404346367635 1104 32-33 0.2026613185646582 1104 34-35 0.16440280825610643 1104 36-37 0.2037503163526111 1104 38-39 0.2754046646499475 1104 40-41 0.4012381016693567 1104 42-43 0.4984570112842235 1104 44-45 0.45802769948845423 1104 46-47 0.6027037777158597 1104 48-49 0.43146514817952664 1104 50-51 0.46595440373044283 1104 52-53 0.5019561640729364 1104 54-55 0.4041717779555327 1104 56-57 0.45652621906472746 1104 58-59 0.4404967432109004 1104 60-61 0.3519643410203699 1104 62-63 0.6529715016986088 1104 64-65 0.5707172876608482 1104 66-67 0.4770279060536211 1104 68-69 0.39177792950074775 1104 70-71 0.2592125892246102 1104 72-73 0.21321756808272951 1104 74-75 0.2793964222355676 1104 76-77 0.07525960227390982 1104 78-79 -0.025584565708932416 1104 80-81 0.05963871043805469 1104 82-83 0.12676939014500022 1104 84-85 0.10818929680241496 1104 86-87 -0.19815007272817198 1104 88-89 -0.14884386734759047 1104 90-91 -0.3282447149852885 1104 92-93 -0.20160506386544697 1104 94-95 -0.061703681151978884 1104 96-97 -0.2650524070867739 1104 98-99 -0.25635344939216154 1104 100-101 -1.1170320608266984 1105 1 0.014758757626793795 1105 2 0.045298118324204495 1105 3 0.08639711395630201 1105 4 -0.06779964970215957 1105 5 0.037489753456235064 1105 6 0.04845203608424242 1105 7 0.055140561323909765 1105 8 0.05882254983317381 1105 9 0.1440544898692977 1105 10-11 0.10788297207170672 1105 12-13 0.03791591425012797 1105 14-15 0.032427303682979414 1105 16-17 0.10397893494072719 1105 18-19 0.11128948155392493 1105 20-21 0.042313217458556096 1105 22-23 0.025478981677530044 1105 24-25 0.09214604666259874 1105 26-27 0.08021336255560385 1105 28-29 0.0899964857906852 1105 30-31 0.121100074411018 1105 32-33 0.09191471905324278 1105 34-35 0.1464546122995003 1105 36-37 0.1056888210424205 1105 38-39 0.09245561417292691 1105 40-41 0.009386205136699743 1105 42-43 -0.08061804576758647 1105 44-45 -0.2600675242450947 1105 46-47 -0.19119720850123656 1105 48-49 -0.10553985830478041 1105 50-51 -0.23148178370107075 1105 52-53 -0.327091498059751 1105 54-55 -0.20617847488197327 1105 56-57 -0.18581551872819801 1105 58-59 -0.31702429307102875 1105 60-61 -0.36049104798748743 1105 62-63 -0.3679104638994062 1105 64-65 -0.4076532927093126 1105 66-67 -0.4862076098253745 1105 68-69 -0.3955442548544923 1105 70-71 -0.5563570240295057 1105 72-73 -0.6123423941173094 1105 74-75 -0.45631876108419434 1105 76-77 -0.5787234681687288 1105 78-79 -0.8228443198081159 1105 80-81 -0.8294324761389404 1105 82-83 -0.6822983280844319 1105 84-85 -0.6350547140148066 1105 86-87 -0.833214563886397 1105 88-89 -0.9761377519973884 1105 90-91 -0.8849270646148248 1105 92-93 -0.5295428547793826 1105 94-95 -0.6256378931333586 1105 96-97 -0.8267326958141403 1105 98-99 -1.0108547613323253 1105 100-101 -1.3656200915090082 1106 1 0.13858909597874458 1106 2 0.15197881553428516 1106 3 0.11629836051612585 1106 4 0.051060638463617636 1106 5 0.07664576230627063 1106 6 0.07664533380157934 1106 7 0.08161781565448933 1106 8 0.05272196162962217 1106 9 0.07230714757800172 1106 10-11 0.09113670905298221 1106 12-13 0.11980706961141152 1106 14-15 0.1954123017942564 1106 16-17 0.20649229093878319 1106 18-19 0.19629838555263035 1106 20-21 0.24880369287811988 1106 22-23 0.3937618590182055 1106 24-25 0.4644208834226191 1106 26-27 0.3982898290172088 1106 28-29 0.27112810308063473 1106 30-31 0.3168329004862471 1106 32-33 0.4927390134788041 1106 34-35 0.5225878484982971 1106 36-37 0.5468131532425176 1106 38-39 0.49172305791584137 1106 40-41 0.5716656288051496 1106 42-43 0.5022687385397901 1106 44-45 0.659227171130226 1106 46-47 0.9290027267424108 1106 48-49 1.0265860388405414 1106 50-51 1.0803726763928267 1106 52-53 0.9753692434005146 1106 54-55 0.9100780682079872 1106 56-57 1.1282352610462354 1106 58-59 0.9362526482705036 1106 60-61 0.7124974577596461 1106 62-63 1.0224867084064613 1106 64-65 0.8269373931684001 1106 66-67 0.6776637408842419 1106 68-69 0.3164909833949281 1106 70-71 -0.19507457839786113 1106 72-73 -0.11931584401208184 1106 74-75 0.16419955956768817 1106 76-77 0.11992474293698407 1106 78-79 0.16380642758813835 1106 80-81 0.2047157079849633 1106 82-83 -0.11319385449963093 1106 84-85 0.1345866976737149 1106 86-87 0.16926236666241223 1106 88-89 -0.06440336097693944 1106 90-91 -0.1909413110127538 1106 92-93 -0.26597370038944135 1106 94-95 -0.30501839067801484 1106 96-97 -0.45907309838280597 1106 98-99 -0.7398332298445496 1106 100-101 -1.4664565277509816 1107 1 0.05791490261857746 1107 2 0.060917979623980045 1107 3 0.05809047207456075 1107 4 0.026155451463210966 1107 5 -0.008313492414927737 1107 6 -0.09915109539498701 1107 7 -0.0033798504870574675 1107 8 -0.01534453388742918 1107 9 -0.03868483950797952 1107 10-11 -0.011032806865877376 1107 12-13 -0.007661228811294052 1107 14-15 -0.0639586261089633 1107 16-17 -0.028285846642177148 1107 18-19 0.16359519400119638 1107 20-21 0.10630388736633023 1107 22-23 0.07148688187056251 1107 24-25 0.030511135673819467 1107 26-27 0.04534795181705675 1107 28-29 -0.029081944179999653 1107 30-31 -0.11764572526011108 1107 32-33 -0.04850853110762188 1107 34-35 0.003949071440125351 1107 36-37 0.1015501565682726 1107 38-39 0.18909892581945797 1107 40-41 0.010435393668913662 1107 42-43 -0.12701559349047642 1107 44-45 -0.11913844724502098 1107 46-47 0.0021028200967521116 1107 48-49 0.019654965460141227 1107 50-51 -0.05327791582375596 1107 52-53 0.03812888515323465 1107 54-55 0.0427124595571442 1107 56-57 0.10313582895179252 1107 58-59 0.011012455338203608 1107 60-61 0.031260221048050596 1107 62-63 0.17156726542067702 1107 64-65 -0.043382005085085495 1107 66-67 0.017268832585433103 1107 68-69 0.011365732991365007 1107 70-71 0.06541587223130918 1107 72-73 -0.06782873858002603 1107 74-75 0.013976870573774391 1107 76-77 -0.08301227205805972 1107 78-79 0.11691313354141997 1107 80-81 0.41686587232749517 1107 82-83 0.28301678595002855 1107 84-85 0.18380923107706337 1107 86-87 0.05173217236869476 1107 88-89 -0.047605900992888195 1107 90-91 -0.5438827019923913 1107 92-93 -0.5769904847214704 1107 94-95 -0.7097016666373257 1107 96-97 -0.9397134994174827 1107 98-99 -1.1351130011264203 1107 100-101 -1.2512701594292182 1108 1 -0.0540904610331836 1108 2 -0.036880415433408587 1108 3 -0.10591180564057368 1108 4 -0.04771615312800037 1108 5 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1.0946714750650912 2316 90-91 1.0672272551793327 2316 92-93 1.3412552177104686 2316 94-95 1.6088825346073214 2316 96-97 1.673245738678773 2316 98-99 2.2948139924788187 2316 100-101 2.5892276857564838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 8.0 5 44.0 6 95.0 7 211.0 8 460.0 9 861.0 10 1519.0 11 2631.0 12 4155.0 13 6565.0 14 8168.0 15 11750.0 16 16983.0 17 25992.0 18 25897.0 19 15386.0 20 11662.0 21 11333.0 22 11906.0 23 12778.0 24 14366.0 25 16530.0 26 18760.0 27 22053.0 28 26437.0 29 32046.0 30 38759.0 31 47475.0 32 59671.0 33 78294.0 34 111338.0 35 177051.0 36 218125.0 37 285106.0 38 175378.0 39 9458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.15096615135037 19.81123894702577 15.754724029179318 19.28307087244454 2 15.399219379038772 21.960651420253853 40.89407850103698 21.746050699670395 3 18.927882694394803 28.477507242548494 30.84215013137646 21.75245993168024 4 11.877302381236648 18.736072323534962 37.73267265316359 31.6539526420648 5 11.895920946876593 39.670700919462796 33.135411490450196 15.297966643210417 6 27.750260131800115 38.264654091406314 17.95016141511699 16.034924361676584 7 25.684486310940773 36.92367516573143 20.20199257902049 17.1898459443073 8 26.594800245187134 34.16718747811424 19.431280569186328 19.80673170751229 9 27.821536314842692 16.932519511253197 20.430771652471915 34.815172521432196 10-11 24.31678097838738 28.783208816598556 25.549753445526235 21.350256759487827 12-13 26.677965660796062 26.945966595931225 26.99439085648577 19.38167688678695 14-15 23.345403708355374 26.881841278014594 28.100706813947916 21.672048199682113 16-17 19.513830108675858 30.669830959779993 28.541737119546113 21.274601811998036 18-19 19.437483616950463 27.33586435468627 33.896287709361204 19.33036431900207 20-21 21.21058813563731 25.846599295787332 34.24022336307906 18.702589205496302 22-23 20.37374363270487 26.479376867167403 33.98873313087831 19.158146369249422 24-25 19.731958384679906 26.72888842938686 34.48545314774639 19.05370003818684 26-27 19.571942254066098 26.85075851579074 34.45679728882307 19.120501941320097 28-29 19.21584806435066 27.09862383026239 34.57897450640725 19.106553598979694 30-31 19.363354974608438 27.147010259011612 34.410627937155255 19.079006829224692 32-33 19.137502893088136 27.27606830822878 34.08187889649781 19.50454990218527 34-35 19.644086428910672 27.23276464718156 34.005735334140915 19.11741358976685 36-37 19.673103218275806 27.09398032349508 33.93113223278306 19.301784225446056 38-39 19.542922472044637 27.152243449179092 33.95921413828052 19.345619940495745 40-41 19.644951074885107 27.153638867952218 33.93795481680596 19.263455240356716 42-43 19.40537488489798 27.336066544224693 33.57820617276992 19.680352398107406 44-45 19.422142746135933 27.685302712065862 33.19490976566487 19.69764477613334 46-47 19.773308316563963 27.875533346095178 32.309918019611 20.04124031772987 48-49 19.76844507157052 28.04598682479404 31.970734808043062 20.21483329559238 50-51 20.12211797444781 28.421377715937634 31.555603432750402 19.900900876864153 52-53 20.06624411667396 28.63782324941033 31.30876655602634 19.987166077889366 54-55 19.695193434935522 28.623367613927247 31.299488542655073 20.381950408482155 56-57 19.950508473897873 28.867188399010168 30.81522930106478 20.36707382602718 58-59 19.941298889655723 28.510033193246336 31.044074533531653 20.50459338356629 60-61 19.929709600195835 28.567831436444152 31.223198366605327 20.27926059675469 62-63 20.097492604164614 28.525125826767507 31.229021228023885 20.14836034104399 64-65 19.778875638727918 28.536990764692284 31.276795384581003 20.407338211998795 66-67 19.68466775243974 28.748727140646746 31.031952480362197 20.53465262655132 68-69 19.918222389464784 28.795933603812596 30.886076456094493 20.399767550628127 70-71 19.78840969721066 28.80618053419914 30.5952720060786 20.810137762511598 72-73 19.82723975486722 28.820144244799266 30.51116021178138 20.841455788552132 74-75 19.839716456831113 29.0553364139901 30.23576563793544 20.869181491243342 76-77 19.77675292384689 28.82675202322666 30.69770715432695 20.6987878985995 78-79 19.73438067077987 28.879914231122306 30.62444626502423 20.76125883307359 80-81 19.63715671589272 29.262736520369327 30.30405818940574 20.79604857433221 82-83 19.763404808967397 29.244259847707692 30.058131291648944 20.934204051675966 84-85 19.85956337960517 28.89137726756008 30.144739580831615 21.104319772003137 86-87 19.855680090310866 28.95075002185048 30.124070687466098 21.06949920037256 88-89 19.944261168614137 28.822479660830382 29.998665737130203 21.234593433425285 90-91 19.7230556150439 28.987592843518517 30.209161027504965 21.080190513932614 92-93 19.77697461360001 28.92772022756195 30.09224898386018 21.203056174977856 94-95 19.545168608882495 28.86943893544025 30.061685281652984 21.523707174024274 96-97 19.73199169078181 28.71083040149214 29.82109431389579 21.73608359383026 98-99 19.679103041436903 28.661296148616128 29.767818983323778 21.891781826623188 100-101 20.08935912047038 28.402538957034444 29.747161583849717 21.76094033864545 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10209.0 1 6200.0 2 3536.0 3 4401.0 4 2950.5 5 2446.0 6 4727.0 7 10307.5 8 15396.0 9 16086.5 10 11667.0 11 6816.0 12 5827.0 13 5887.0 14 5787.5 15 5573.0 16 5375.5 17 5402.5 18 5505.5 19 5574.0 20 5804.5 21 6219.0 22 6977.0 23 7947.5 24 9004.5 25 10616.5 26 12675.0 27 14746.5 28 16399.5 29 18403.5 30 20938.0 31 23161.5 32 25972.0 33 28699.0 34 31220.5 35 33751.5 36 36770.0 37 39805.0 38 41335.5 39 42186.0 40 42656.5 41 43495.0 42 44483.5 43 46170.5 44 48213.0 45 49524.5 46 49684.0 47 50491.0 48 56383.0 49 56471.0 50 50886.0 51 46999.0 52 42173.0 53 39690.5 54 38073.5 55 34932.5 56 31534.5 57 28352.0 58 25522.5 59 22519.0 60 19065.5 61 16171.0 62 13254.5 63 9477.0 64 6350.5 65 4789.5 66 3605.5 67 2701.0 68 2042.5 69 1486.0 70 1066.5 71 808.5 72 677.5 73 656.0 74 531.0 75 402.5 76 305.5 77 182.0 78 106.0 79 58.0 80 31.0 81 18.0 82 9.0 83 4.5 84 2.0 85 1.0 86 0.5 87 0.5 88 0.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007937233160559555 2 0.013339887664805974 3 0.009071123612068063 4 0.0 5 0.0012005898898325377 6 0.001534087081452687 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.671418556984333E-4 14-15 0.0 16-17 0.002267780903017016 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003701818826983658 26-27 0.018875941045700454 28-29 0.0068033427090510475 30-31 0.0029681250054193293 32-33 0.0 34-35 0.009004424173744034 36-37 8.670926982123884E-4 38-39 0.0028347261287712698 40-41 0.0022010814646929856 42-43 0.0033016221970394786 44-45 0.0 46-47 1.3339887664805977E-4 48-49 5.002457874302241E-4 50-51 0.0038018679844697024 52-53 0.007403637653967316 54-55 0.007703785126425451 56-57 0.014573827273800527 58-59 0.020910273914583364 60-61 0.003601769669497613 62-63 0.018509094134918292 64-65 0.019176088518158586 66-67 0.011839150302515302 68-69 0.029481151739221206 70-71 0.015607668567822992 72-73 0.005035807593464256 74-75 0.015474269691174932 76-77 0.019743033743912845 78-79 0.023411502851734486 80-81 0.010571860974358735 82-83 0.03338306888117695 84-85 0.019676334305588815 86-87 0.029347752862573146 88-89 0.0205434270038012 90-91 0.02467879217989105 92-93 0.022044164366091874 94-95 0.013473286541454034 96-97 0.013673384856426125 98-99 0.010171664344414555 100-101 0.009571369399498287 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1499263.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.27672972267434 #Duplication Level Percentage of deduplicated Percentage of total 1 73.86250809574173 37.87427864264194 2 14.440321058982214 14.80904880100147 3 5.092185612286481 7.833318760167145 4 2.286287378667739 4.689333599372291 5 1.2188701853195194 3.1249838529827487 6 0.7315065566091001 2.2505558396165406 7 0.4865657423963112 1.7464650045617627 8 0.3353302986935448 1.3755712875146044 9 0.24040605860992942 1.1094512841931283 >10 1.180792090250825 10.871021625087293 >50 0.08103399311427854 2.8294515262220243 >100 0.039009079291019655 3.70364374234241 >500 0.0034990987752208254 1.2468523726424323 >1k 0.0011663662584069417 1.8214025839021708 >5k 1.2959625093410463E-4 0.6215987365398556 >10k+ 3.887887528023139E-4 4.093022341212245 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25409 1.694766028375275 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21408 1.4279015756408315 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14776 0.9855509006758655 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9354 0.6239065460829754 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4845 0.32315877867992476 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4229 0.28207192467232234 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4074 0.2717335117320977 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3378 0.22531070265857292 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2563 0.17095066042448856 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2541 0.1694832727813599 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2317 0.15454259859677721 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1939 0.12933021091029392 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1523 0.1015832445674975 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3339887664805974E-4 0.0 20-21 0.0 0.0 0.0 5.002457874302241E-4 0.0 22-23 0.0 0.0 0.0 0.0013339887664805975 0.0 24-25 0.0 0.0 0.0 0.003001474724581344 0.0 26-27 0.0 0.0 0.0 0.006102998606648733 0.0 28-29 0.0 0.0 0.0 0.014073581486370303 0.0 30-31 0.0 0.0 0.0 0.03441691017519941 0.0 32-33 0.0 0.0 0.0 0.06399811107190667 0.0 34-35 6.669943832402987E-5 0.0 0.0 0.10668575159928578 0.0 36-37 6.669943832402987E-5 0.0 0.0 0.17158430508856684 0.0 38-39 6.669943832402987E-5 0.0 0.0 0.2649635187422087 0.0 40-41 6.669943832402987E-5 0.0 0.0 0.37268311163551693 0.0 42-43 6.669943832402987E-5 0.0 0.0 0.4742330064838524 0.0 44-45 6.669943832402987E-5 0.0 0.0 0.5887225923670496 0.0 46-47 6.669943832402987E-5 0.0 0.0 0.7262234844720372 0.0 48-49 6.669943832402987E-5 0.0 0.0 0.8706277684435619 0.0 50-51 6.669943832402987E-5 0.0 0.0 1.0230693347331323 0.0 52-53 6.669943832402987E-5 0.0 0.0 1.168140613087897 0.0 54-55 6.669943832402987E-5 0.0 0.0 1.3187812945427186 0.0 56-57 6.669943832402987E-5 0.0 0.0 1.4971689423403367 0.0 58-59 6.669943832402987E-5 0.0 0.0 1.6940656842728727 0.0 60-61 6.669943832402987E-5 0.0 0.0 1.9087044767996009 0.0 62-63 6.669943832402987E-5 0.0 0.0 2.1160730305490096 0.0 64-65 6.669943832402987E-5 0.0 0.0 2.3197064157522727 0.0 66-67 6.669943832402987E-5 0.0 0.0 2.5377135299143645 0.0 68-69 6.669943832402987E-5 0.0 0.0 2.784468101994113 0.0 70-71 6.669943832402987E-5 0.0 0.0 3.0558681165345907 0.0 72-73 6.669943832402987E-5 0.0 0.0 3.3280018248966323 0.0 74-75 6.669943832402987E-5 0.0 0.0 3.5908309616124723 0.0 76-77 6.669943832402987E-5 0.0 0.0 3.8652658005966933 0.0 78-79 6.669943832402987E-5 0.0 0.0 4.173884101721979 0.0 80-81 6.669943832402987E-5 0.0 0.0 4.499644158496541 0.0 82-83 6.669943832402987E-5 0.0 0.0 4.8359093768071375 0.0 84-85 6.669943832402987E-5 0.0 0.0 5.181946062832205 0.0 86-87 6.669943832402987E-5 0.0 0.0 5.525514869639283 0.0 88-89 6.669943832402987E-5 0.0 0.0 5.890227398395078 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12205 0.0 39.002403 1 GCGCGAC 60 0.00395541 31.659996 9 GTATCAA 18515 0.0 30.99593 1 GTGTAGC 975 0.0 26.79906 1 TATCACG 145 7.88179E-7 26.210117 2 TATCAAC 22645 0.0 25.300083 2 ATCAACG 22830 0.0 25.050943 3 TCAACGC 23090 0.0 24.764732 4 CAACGCA 23355 0.0 24.524408 5 AACGCAG 23890 0.0 24.01496 6 GAGTACT 14480 0.0 23.910578 12-13 GTACATG 14760 0.0 23.850271 1 TACATGG 14660 0.0 23.623299 2 TAGCCCT 1045 0.0 23.178482 4 GTACTTT 15240 0.0 22.609114 14-15 ACATGGG 14880 0.0 22.314016 3 AGTACTT 14550 0.0 21.918665 12-13 ACGCAGA 26575 0.0 21.533564 7 CGCAGAG 26665 0.0 21.443073 8 AAGGCGC 135 3.8003802E-4 21.108074 6 >>END_MODULE