FastQCFastQC Report
Thu 26 May 2016
SRR938191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3446066
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147030.4266604296029153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118370.34349313100793777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74360.21578228623595716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78900.046.4073751
GTATCAA128050.039.243151
ATCAACG160600.031.1307473
TATCAAC161700.031.0071472
TCAACGC162500.030.7667564
CAACGCA165150.030.2442995
AACGCAG170500.029.2614576
ACGCAGA189400.026.1845057
CGCAGAG192350.025.684148
GTACATG105050.023.8722041
GCAGAGT206150.023.3883889
TACATGG103200.023.3478892
GTGGTAT38050.022.9297471
GAGTACT133650.022.62250512-13
ACATGGG102500.022.4872953
GGACCGA15950.022.0445946
GTACTTT140350.021.4258114-15
CAGAGTA199700.020.68293810-11
GTGTAGC18100.020.54561
AGAGTAC186850.020.51640110-11
Produced by FastQC (version 0.11.5)