##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938191_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3446066 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.872770863935862 33.0 31.0 34.0 30.0 34.0 2 32.040597017004316 33.0 31.0 34.0 30.0 34.0 3 32.12743661903167 34.0 31.0 34.0 30.0 34.0 4 35.6558913845527 37.0 35.0 37.0 33.0 37.0 5 35.66591295697761 37.0 35.0 37.0 35.0 37.0 6 35.70013313732238 37.0 35.0 37.0 35.0 37.0 7 35.63113300789944 37.0 35.0 37.0 33.0 37.0 8 35.60404733977817 37.0 35.0 37.0 33.0 37.0 9 37.23994926388525 39.0 37.0 39.0 34.0 39.0 10-11 37.13271408614925 39.0 37.0 39.0 33.5 39.0 12-13 36.994274485746935 39.0 37.0 39.0 33.0 39.0 14-15 38.144076753027946 40.0 38.0 41.0 33.0 41.0 16-17 37.963584707895905 40.0 38.0 41.0 33.0 41.0 18-19 38.1611317078663 40.0 38.0 41.0 33.0 41.0 20-21 38.14763501337467 40.0 38.0 41.0 33.0 41.0 22-23 38.03467736833828 40.0 38.0 41.0 33.0 41.0 24-25 37.902481264142935 40.0 38.0 41.0 33.0 41.0 26-27 37.69095441004322 40.0 38.0 41.0 32.0 41.0 28-29 37.472417823686484 40.0 37.0 41.0 32.0 41.0 30-31 37.215975259905065 40.0 37.0 41.0 31.0 41.0 32-33 36.89046074567347 39.5 36.0 41.0 30.5 41.0 34-35 36.576815272835745 39.0 35.5 41.0 30.0 41.0 36-37 36.258954123339485 39.0 35.0 40.5 30.0 41.0 38-39 35.917214441046696 39.0 35.0 40.0 28.5 41.0 40-41 35.75297875896747 39.0 35.0 40.0 27.5 41.0 42-43 35.47273064416062 38.5 35.0 40.0 26.5 41.0 44-45 35.09362023246217 38.0 34.5 40.0 25.5 41.0 46-47 34.61407123369082 38.0 34.0 40.0 24.0 41.0 48-49 34.120134089132364 38.0 33.0 40.0 22.5 41.0 50-51 33.28476819654644 37.5 32.5 39.5 19.5 40.5 52-53 33.24495511693624 37.5 33.0 39.5 18.5 40.5 54-55 33.2007499275986 38.0 32.5 40.0 15.5 41.0 56-57 32.70474694912981 37.0 31.5 40.0 10.0 41.0 58-59 32.21781982701434 37.0 31.0 40.0 7.5 41.0 60-61 31.62897344392127 36.0 30.5 39.5 2.0 41.0 62-63 30.975081150506114 35.5 29.5 39.0 2.0 40.5 64-65 30.347589251047424 35.0 28.5 39.0 2.0 40.0 66-67 29.630368658058202 35.0 28.0 38.0 2.0 40.0 68-69 28.893670347578947 34.0 26.5 38.0 2.0 40.0 70-71 28.019768048551597 34.0 25.5 37.0 2.0 39.5 72-73 27.17411694958831 33.5 22.5 36.5 2.0 39.0 74-75 26.310231870196333 33.0 20.0 36.0 2.0 39.0 76-77 25.426485882742814 32.0 15.5 35.0 2.0 38.0 78-79 24.549561006666732 31.0 7.5 35.0 2.0 37.0 80-81 23.70883262247444 31.0 2.0 35.0 2.0 36.5 82-83 22.837675627802835 30.0 2.0 34.5 2.0 36.0 84-85 21.99120257708355 29.5 2.0 34.0 2.0 35.5 86-87 21.190046563240518 29.0 2.0 34.0 2.0 35.0 88-89 20.33179820119522 28.0 2.0 34.0 2.0 35.0 90-91 19.45581991174864 26.0 2.0 34.0 2.0 35.0 92-93 18.533931010027086 25.0 2.0 33.5 2.0 35.0 94-95 17.586140689122033 21.5 2.0 33.0 2.0 35.0 96-97 16.47472451195073 10.0 2.0 32.5 2.0 34.5 98-99 15.169279114213134 2.0 2.0 32.0 2.0 34.0 100-101 12.210762359165495 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4786831429272631 1101 2 0.4973661218410541 1101 3 0.4099063774344103 1101 4 0.34359251592313456 1101 5 0.30775744574353325 1101 6 0.30389091983208516 1101 7 0.3391667334825925 1101 8 0.3393007960129779 1101 9 0.36055019485911544 1101 10-11 0.3471364772410297 1101 12-13 0.412023591258702 1101 14-15 0.5465172297321459 1101 16-17 0.6216056768233571 1101 18-19 0.7019670452894431 1101 20-21 0.6978681687470711 1101 22-23 0.6946211974378826 1101 24-25 0.7519101947319271 1101 26-27 0.8854518549661563 1101 28-29 0.9035319581183288 1101 30-31 0.8754353688007583 1101 32-33 1.0200360613047081 1101 34-35 1.0881682938383364 1101 36-37 1.1142609612761305 1101 38-39 1.2191658877848894 1101 40-41 1.253293806926031 1101 42-43 1.4418772535088564 1101 44-45 1.474927918951117 1101 46-47 1.562733554156587 1101 48-49 1.7925716049559028 1101 50-51 1.9110671546286184 1101 52-53 2.0525212642652377 1101 54-55 2.2546154673595353 1101 56-57 2.3293391558063234 1101 58-59 2.4876262869758747 1101 60-61 2.6887567926593334 1101 62-63 2.991534122534013 1101 64-65 3.034980190273572 1101 66-67 3.1580594919851457 1101 68-69 3.492614753872683 1101 70-71 3.7653294279362335 1101 72-73 4.112477881831129 1101 74-75 4.301638360595259 1101 76-77 4.667186339885742 1101 78-79 4.935769964652128 1101 80-81 5.046390796601791 1101 82-83 5.637584304031947 1101 84-85 5.763458733547477 1101 86-87 5.934718810589189 1101 88-89 5.988792149493424 1101 90-91 6.076134549092821 1101 92-93 6.251727406619555 1101 94-95 6.210317107313767 1101 96-97 6.134854308356971 1101 98-99 6.249238326697808 1101 100-101 5.186257931968701 1102 1 0.43978578246435873 1102 2 0.340605035631242 1102 3 0.28754193545442064 1102 4 0.2258335296368159 1102 5 0.2920352312227408 1102 6 0.31015372346099923 1102 7 0.37803534193670885 1102 8 0.3788840972570213 1102 9 0.4178478140901021 1102 10-11 0.46977611701944255 1102 12-13 0.5024614946211443 1102 14-15 0.6724001987877983 1102 16-17 0.7026806938948695 1102 18-19 0.6072902743454023 1102 20-21 0.5573365646416946 1102 22-23 0.680796615497222 1102 24-25 0.6276707110358615 1102 26-27 0.6592295095210332 1102 28-29 0.6779395897229463 1102 30-31 0.7209266944223955 1102 32-33 0.7749390489464716 1102 34-35 0.9196589314654773 1102 36-37 1.000160668108336 1102 38-39 1.1970571333116382 1102 40-41 1.1951320871451614 1102 42-43 1.245313631987976 1102 44-45 1.5151689223962208 1102 46-47 1.5700956086929878 1102 48-49 1.6154037805074566 1102 50-51 1.794455168121523 1102 52-53 1.8052360186665979 1102 54-55 1.9009919441089096 1102 56-57 2.2155263827264164 1102 58-59 2.3528681889212564 1102 60-61 2.5769463916201545 1102 62-63 2.799705340746357 1102 64-65 2.6298746549984457 1102 66-67 3.0331730880912993 1102 68-69 3.2749651279055136 1102 70-71 3.5240677281790838 1102 72-73 3.699648324766091 1102 74-75 3.8824659050196786 1102 76-77 4.223378547064502 1102 78-79 4.466150569368484 1102 80-81 4.670152278151729 1102 82-83 5.0345843399719605 1102 84-85 5.13345703923267 1102 86-87 5.212470403501818 1102 88-89 5.175373689676924 1102 90-91 5.341576174813547 1102 92-93 5.644533756706064 1102 94-95 5.571951145584741 1102 96-97 5.610317549427162 1102 98-99 5.736061691812383 1102 100-101 4.774216488001741 1103 1 0.5035732350812587 1103 2 0.38434578695086685 1103 3 0.3021245526958154 1103 4 0.2773161963120856 1103 5 0.27486849704844474 1103 6 0.2505497001135737 1103 7 0.2505963126265627 1103 8 0.3400951250459556 1103 9 0.39064476564974626 1103 10-11 0.38560759859954175 1103 12-13 0.5030663737824739 1103 14-15 0.6611618720949579 1103 16-17 0.6523286590641604 1103 18-19 0.6334873245576347 1103 20-21 0.6521464378618234 1103 22-23 0.6707030676671266 1103 24-25 0.6988641007743723 1103 26-27 0.8233051202230683 1103 28-29 0.8828620527672228 1103 30-31 0.9091672948584275 1103 32-33 1.0720588666597237 1103 34-35 1.1596240426594306 1103 36-37 1.1781652285045396 1103 38-39 1.3030194290875912 1103 40-41 1.4252675488785513 1103 42-43 1.4124130467963028 1103 44-45 1.5175496019802424 1103 46-47 1.7620962498810329 1103 48-49 1.8924732929301982 1103 50-51 2.0075791363439848 1103 52-53 2.0539284953038504 1103 54-55 2.1234577855149297 1103 56-57 2.3193933060834624 1103 58-59 2.6462198972409396 1103 60-61 2.723790396269848 1103 62-63 2.7734684314448828 1103 64-65 2.9467568350808477 1103 66-67 3.2300500934849232 1103 68-69 3.43781123900855 1103 70-71 3.530604027114723 1103 72-73 3.9498780927818906 1103 74-75 4.0590485556114615 1103 76-77 4.229390760836168 1103 78-79 4.439191247693799 1103 80-81 4.761275266303198 1103 82-83 5.095279727775075 1103 84-85 5.080423633442937 1103 86-87 5.181658735815816 1103 88-89 5.536994443083639 1103 90-91 5.811799498674294 1103 92-93 5.713491325871807 1103 94-95 5.656153552299141 1103 96-97 5.574378744156295 1103 98-99 5.541221695663809 1103 100-101 4.672539716706314 1104 1 0.5579434140284043 1104 2 0.5508845483080549 1104 3 0.42255240774530733 1104 4 0.41790907689001244 1104 5 0.4293761289255471 1104 6 0.41702643952668694 1104 7 0.39568665553307625 1104 8 0.4555955672982961 1104 9 0.5699673260960267 1104 10-11 0.6010635798317949 1104 12-13 0.6410256795959697 1104 14-15 0.8333305344671729 1104 16-17 0.8429788868743415 1104 18-19 0.711421587857906 1104 20-21 0.7872369468436275 1104 22-23 0.78825254067538 1104 24-25 0.8679096763441692 1104 26-27 0.9566374864924754 1104 28-29 1.0010837718883963 1104 30-31 1.0091230388695678 1104 32-33 0.9901010706961344 1104 34-35 1.0929901263129835 1104 36-37 1.2312039347083328 1104 38-39 1.3543277230515187 1104 40-41 1.3617962903600187 1104 42-43 1.3827139670174304 1104 44-45 1.3775109207902645 1104 46-47 1.6965539197504285 1104 48-49 1.7827347405408247 1104 50-51 1.8244229021442067 1104 52-53 1.8598979177678103 1104 54-55 1.9202711800405083 1104 56-57 2.2293005376576147 1104 58-59 2.414174122380807 1104 60-61 2.4220618908203164 1104 62-63 2.66087850987752 1104 64-65 2.8320606277893603 1104 66-67 3.1071895942667034 1104 68-69 3.199988504490765 1104 70-71 3.4863040078007828 1104 72-73 3.8440499470801406 1104 74-75 4.16509977528068 1104 76-77 4.319277319112398 1104 78-79 4.357465905875998 1104 80-81 4.638748404137896 1104 82-83 4.920980739768172 1104 84-85 4.965769560067184 1104 86-87 5.0046086901553615 1104 88-89 5.2152903096952485 1104 90-91 5.354116507285195 1104 92-93 5.367791414896363 1104 94-95 5.3036747966090125 1104 96-97 5.251896055967364 1104 98-99 5.229133731529513 1104 100-101 4.36660022880049 1105 1 0.5006855285815099 1105 2 0.5203381755300214 1105 3 0.5206201616718431 1105 4 0.44155977893987597 1105 5 0.37084216038294926 1105 6 0.422394427048566 1105 7 0.4181537287782575 1105 8 0.4320876784487737 1105 9 0.534840723020281 1105 10-11 0.5588346692291637 1105 12-13 0.5803836851654012 1105 14-15 0.7669644430846176 1105 16-17 0.8228991953586373 1105 18-19 0.772871269196834 1105 20-21 0.845874598352971 1105 22-23 0.7947889423893741 1105 24-25 0.8760081278174283 1105 26-27 0.9212278332923134 1105 28-29 1.0885423230061875 1105 30-31 1.135548448769697 1105 32-33 1.1533602312539486 1105 34-35 1.324827184326061 1105 36-37 1.3469407633258754 1105 38-39 1.35930990105296 1105 40-41 1.5013627507042244 1105 42-43 1.5547999847009848 1105 44-45 1.7311122244443737 1105 46-47 1.9088482211051385 1105 48-49 1.8417434244389241 1105 50-51 1.9772425538627658 1105 52-53 2.018399668274604 1105 54-55 2.1941663366800483 1105 56-57 2.581415281307436 1105 58-59 2.835856150783229 1105 60-61 2.9954508140379517 1105 62-63 3.100944505325387 1105 64-65 3.1557656552417654 1105 66-67 3.270762185565072 1105 68-69 3.371382864915116 1105 70-71 3.6940323212906314 1105 72-73 3.837154324960956 1105 74-75 4.175748594412866 1105 76-77 4.474181081097914 1105 78-79 4.584144675532013 1105 80-81 4.755501549430935 1105 82-83 4.95434312831263 1105 84-85 5.097610740122256 1105 86-87 5.26170322024452 1105 88-89 5.257054080912585 1105 90-91 5.409016273612078 1105 92-93 5.322608827879474 1105 94-95 5.428803610421429 1105 96-97 5.366263034755573 1105 98-99 5.169877336239685 1105 100-101 4.343340926304435 1106 1 0.4232408094875133 1106 2 0.39190250511833824 1106 3 0.34005544703322244 1106 4 0.3090851373059067 1106 5 0.3219498070247866 1106 6 0.2624005826900273 1106 7 0.2314696094480695 1106 8 0.3381862078073823 1106 9 0.3812051878925544 1106 10-11 0.32002039920562453 1106 12-13 0.3734989929806716 1106 14-15 0.6046150239425998 1106 16-17 0.5422074307143632 1106 18-19 0.5009535526779629 1106 20-21 0.5575346151565057 1106 22-23 0.4927543627322777 1106 24-25 0.5809700118936831 1106 26-27 0.6854024517642543 1106 28-29 0.6426321640217125 1106 30-31 0.6975431624420096 1106 32-33 0.7142819030214227 1106 34-35 0.8678281572788578 1106 36-37 1.0209541825505113 1106 38-39 1.0809632919668033 1106 40-41 1.2656702834537867 1106 42-43 1.141326672301993 1106 44-45 1.4529555587587737 1106 46-47 1.4756583227008875 1106 48-49 1.669911219332512 1106 50-51 1.7577175432960175 1106 52-53 1.8198549205600116 1106 54-55 1.9149495839671786 1106 56-57 1.9038977751647082 1106 58-59 2.280235697899151 1106 60-61 2.5988912238140998 1106 62-63 2.481238025046679 1106 64-65 2.7514967602992755 1106 66-67 2.923346024369142 1106 68-69 2.802886969536683 1106 70-71 3.24345296625728 1106 72-73 3.4780071302590123 1106 74-75 3.747938750623792 1106 76-77 3.692586762581641 1106 78-79 4.106836826333062 1106 80-81 4.160338338258871 1106 82-83 4.283734721159544 1106 84-85 4.1631216657404195 1106 86-87 4.158608120194138 1106 88-89 4.537188416999545 1106 90-91 4.777613613519296 1106 92-93 4.698503267664467 1106 94-95 4.589823564067867 1106 96-97 4.479945727481731 1106 98-99 4.272660919892603 1106 100-101 3.180170232468857 1107 1 0.5519868554948282 1107 2 0.461466543951083 1107 3 0.46531941088493056 1107 4 0.2757513667653626 1107 5 0.3155605701918276 1107 6 0.3967109101800901 1107 7 0.3489592093816185 1107 8 0.3939615510696939 1107 9 0.4518116926613942 1107 10-11 0.488929665593794 1107 12-13 0.5195879559033045 1107 14-15 0.6550856775017948 1107 16-17 0.7469683712782356 1107 18-19 0.6224263835290529 1107 20-21 0.6157421967666963 1107 22-23 0.6610516756567719 1107 24-25 0.63927555524387 1107 26-27 0.8041043453086729 1107 28-29 0.8484945934947277 1107 30-31 0.8154064379109727 1107 32-33 0.9346868006165678 1107 34-35 1.0577728287067174 1107 36-37 1.1225868235662304 1107 38-39 1.1042378810466786 1107 40-41 1.086342096049286 1107 42-43 1.0870219847849185 1107 44-45 1.1715568399945013 1107 46-47 1.4887368298131562 1107 48-49 1.541595822424874 1107 50-51 1.6816291432776538 1107 52-53 1.7391217404678017 1107 54-55 1.7906194673858735 1107 56-57 1.9882183770241468 1107 58-59 2.02015738862314 1107 60-61 2.1334049073066836 1107 62-63 2.3013980319545553 1107 64-65 2.4898205953060284 1107 66-67 2.6103185065474896 1107 68-69 2.8338201992519068 1107 70-71 3.056901432643695 1107 72-73 3.5352539490300643 1107 74-75 3.5966936118214363 1107 76-77 3.6942609929330033 1107 78-79 3.8637594559595314 1107 80-81 4.068454598213531 1107 82-83 4.312187209852272 1107 84-85 4.437939186382064 1107 86-87 4.590918334010322 1107 88-89 4.654729115525502 1107 90-91 4.6333379674909985 1107 92-93 4.751709062190191 1107 94-95 4.7979619303119705 1107 96-97 4.762414101005341 1107 98-99 4.713795478061927 1107 100-101 3.75747037958908 1108 1 0.4404373422519505 1108 2 0.4081215224208634 1108 3 0.45972033252426314 1108 4 0.2492127358818621 1108 5 0.30352843560929443 1108 6 0.21381223166832086 1108 7 0.261306544390294 1108 8 0.3231760456845123 1108 9 0.3846921083655275 1108 10-11 0.45684312063558963 1108 12-13 0.43444873645997006 1108 14-15 0.6035601148296692 1108 16-17 0.629315527211908 1108 18-19 0.6394211696040628 1108 20-21 0.5780237430310109 1108 22-23 0.5676583777802975 1108 24-25 0.632586109100103 1108 26-27 0.6956583723785599 1108 28-29 0.7155915233817538 1108 30-31 0.7634549325581901 1108 32-33 0.8211243990900599 1108 34-35 1.0319408334615758 1108 36-37 1.1146313455908938 1108 38-39 1.2014633839418636 1108 40-41 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2311 50-51 1.1328449237997447 2311 52-53 1.1046731566669763 2311 54-55 1.3150704958381496 2311 56-57 1.6178522566147322 2311 58-59 1.6434579816827508 2311 60-61 1.7270709318068924 2311 62-63 2.0071006031624563 2311 64-65 2.259418491717895 2311 66-67 2.3451770505567957 2311 68-69 2.528318117972116 2311 70-71 3.0685014106917237 2311 72-73 3.2754151772381626 2311 74-75 3.598444780514498 2311 76-77 3.6574351340799076 2311 78-79 3.9678062212627765 2311 80-81 4.056603265332068 2311 82-83 4.273346354761184 2311 84-85 4.519582704884083 2311 86-87 4.542807640952823 2311 88-89 4.647969143473876 2311 90-91 4.87466794815948 2311 92-93 5.110235609703263 2311 94-95 5.1921044836636945 2311 96-97 5.099965349001092 2311 98-99 4.826414203426838 2311 100-101 4.230697051045269 2312 1 -0.26782130680584615 2312 2 -0.2139148817884653 2312 3 -0.13686437765124992 2312 4 -0.05703557156917327 2312 5 -0.2360296597419378 2312 6 -0.1664601835722408 2312 7 -0.09578151482258335 2312 8 -0.05226401704674544 2312 9 -0.11793359631817424 2312 10-11 -0.19264044717395734 2312 12-13 -0.090745937605476 2312 14-15 -0.021921841802900133 2312 16-17 0.009886915244656791 2312 18-19 -0.06678567997220597 2312 20-21 -0.05561568093315117 2312 22-23 -0.07547982542740073 2312 24-25 0.03941584026499356 2312 26-27 -0.06159870014868574 2312 28-29 0.10725672287803434 2312 30-31 0.13182759430694801 2312 32-33 0.2073803947495847 2312 34-35 0.1608445378252128 2312 36-37 0.2713647384595248 2312 38-39 0.23162428244879152 2312 40-41 0.49547918010063796 2312 42-43 0.5824995843125222 2312 44-45 0.7047410917835961 2312 46-47 0.7046710309016717 2312 48-49 0.8904762638955006 2312 50-51 0.8977756781939945 2312 52-53 1.0862478153145005 2312 54-55 1.2387919839700743 2312 56-57 1.4246786561288687 2312 58-59 1.5642222274963373 2312 60-61 1.8117771509000988 2312 62-63 2.018494614333079 2312 64-65 2.1413798651532687 2312 66-67 2.1551444858267352 2312 68-69 2.3697624940603745 2312 70-71 2.8130182890557265 2312 72-73 3.1132259393387294 2312 74-75 2.8303402897099836 2312 76-77 3.1193258701617914 2312 78-79 3.425240606710119 2312 80-81 3.8027026780795303 2312 82-83 4.125232384706713 2312 84-85 4.438446077412895 2312 86-87 4.739826818704714 2312 88-89 4.694156946484345 2312 90-91 4.846357258059449 2312 92-93 5.023662301435177 2312 94-95 5.001606722575072 2312 96-97 4.998181705592668 2312 98-99 4.854683623725004 2312 100-101 4.3847759356614056 2313 1 -0.5368311329585289 2313 2 -0.3293746953435175 2313 3 -0.3619034303093507 2313 4 -0.25631498703906175 2313 5 -0.26683338864090445 2313 6 -0.3300379109594971 2313 7 -0.27402288544490006 2313 8 -0.20257895783787916 2313 9 -0.29285423123880605 2313 10-11 -0.23534641845135695 2313 12-13 -0.12849852011996177 2313 14-15 -0.25128692116798135 2313 16-17 -0.07634639289341294 2313 18-19 -0.14547552628109628 2313 20-21 -0.20555269277492272 2313 22-23 -0.14575949284992618 2313 24-25 -0.12360255227720529 2313 26-27 -0.05665035043842437 2313 28-29 0.012905500907763212 2313 30-31 0.12867314734297253 2313 32-33 0.19039878729179094 2313 34-35 0.22419550784285036 2313 36-37 0.34377592515642164 2313 38-39 0.36054340565362963 2313 40-41 0.6043655494632034 2313 42-43 0.4616983749398855 2313 44-45 0.6252979071023219 2313 46-47 0.6675785642853995 2313 48-49 0.9735500860169424 2313 50-51 1.0373775930340017 2313 52-53 1.1279686517972394 2313 54-55 1.1815760605636783 2313 56-57 1.4976214628811988 2313 58-59 1.7312466669017255 2313 60-61 1.9344201340192697 2313 62-63 1.965264581579234 2313 64-65 2.158870416929531 2313 66-67 2.2128819511832525 2313 68-69 2.525698498091618 2313 70-71 2.8235020381109095 2313 72-73 3.1097515125309734 2313 74-75 3.3188764449128065 2313 76-77 3.594218790292807 2313 78-79 3.7221667845886763 2313 80-81 3.904917341722765 2313 82-83 4.095131603653552 2313 84-85 4.250726248740687 2313 86-87 4.474913742363068 2313 88-89 4.569193479616118 2313 90-91 4.731071291821099 2313 92-93 4.857509618139641 2313 94-95 4.944267342378549 2313 96-97 4.946906825270169 2313 98-99 4.672872084294413 2313 100-101 4.129915769372666 2314 1 -0.2975097756730989 2314 2 -0.2896127377771691 2314 3 -0.17053653447139894 2314 4 -0.1747285303228665 2314 5 -0.05924707977714405 2314 6 -0.14182470773631195 2314 7 -0.1808433186525633 2314 8 -0.09944005046743598 2314 9 -0.19089370784115545 2314 10-11 -0.21617734918248743 2314 12-13 -0.25523763448383363 2314 14-15 -0.26242686350553157 2314 16-17 -0.16457881693421683 2314 18-19 -0.20426313624634673 2314 20-21 -0.24994538886422646 2314 22-23 -0.208788989412362 2314 24-25 -0.11674811300760268 2314 26-27 -0.1610652243942141 2314 28-29 -0.1304714488963299 2314 30-31 0.03156809818204209 2314 32-33 0.004758216581357999 2314 34-35 0.16878336915726067 2314 36-37 0.3257053995409933 2314 38-39 0.2806247244974216 2314 40-41 0.44736473627475704 2314 42-43 0.5087243230436798 2314 44-45 0.7083636275133571 2314 46-47 0.8621775145330517 2314 48-49 0.8767850974755049 2314 50-51 0.9643917129423372 2314 52-53 1.2842213316227742 2314 54-55 1.4885303002324903 2314 56-57 1.697038184992529 2314 58-59 1.906231512026288 2314 60-61 2.0677645562858515 2314 62-63 2.264740444667872 2314 64-65 2.4512885436390803 2314 66-67 2.492698614153607 2314 68-69 2.8670373019653503 2314 70-71 3.188744146486748 2314 72-73 3.503465565905703 2314 74-75 3.672353195116102 2314 76-77 3.798022294395711 2314 78-79 3.901486663349676 2314 80-81 4.2538483685579855 2314 82-83 4.515710445968917 2314 84-85 4.727474973513363 2314 86-87 4.9427868849667504 2314 88-89 5.063387314169233 2314 90-91 5.145472859056998 2314 92-93 5.312309647710126 2314 94-95 5.163819349475464 2314 96-97 5.120295603269728 2314 98-99 5.214200538497119 2314 100-101 4.663626126436316 2315 1 -0.5764084624494039 2315 2 -0.5608373203123875 2315 3 -0.47725583362525015 2315 4 -0.40395365356341983 2315 5 -0.3700539386818136 2315 6 -0.3578879127384127 2315 7 -0.43368702605044973 2315 8 -0.4027942067082648 2315 9 -0.5379433549446446 2315 10-11 -0.4310361042947406 2315 12-13 -0.46106620788457775 2315 14-15 -0.44241190782610573 2315 16-17 -0.43758985537606776 2315 18-19 -0.5122583561811211 2315 20-21 -0.5944887229453499 2315 22-23 -0.44354314105144255 2315 24-25 -0.403962071258249 2315 26-27 -0.41746064680587835 2315 28-29 -0.3302006648160756 2315 30-31 -0.3681004321923709 2315 32-33 -0.31011443005620265 2315 34-35 -0.25716838727224456 2315 36-37 -0.19314370340585185 2315 38-39 -0.047826039680266774 2315 40-41 0.015432721342094169 2315 42-43 0.08988281298022827 2315 44-45 0.18927414078076055 2315 46-47 0.23837178305652174 2315 48-49 0.4258338587425996 2315 50-51 0.5822822431211705 2315 52-53 0.6881664672880419 2315 54-55 0.8137813830811993 2315 56-57 1.0551505481624517 2315 58-59 1.3282150377701925 2315 60-61 1.5480373823915627 2315 62-63 1.5767958727166729 2315 64-65 1.7183659162713383 2315 66-67 1.9865289074574868 2315 68-69 2.278621079654929 2315 70-71 2.565983270544109 2315 72-73 2.7924771680616196 2315 74-75 3.2001382302331898 2315 76-77 3.196913848463371 2315 78-79 3.6241529446199863 2315 80-81 3.8344653190949494 2315 82-83 3.953157540252974 2315 84-85 4.251519823295066 2315 86-87 4.408371421944668 2315 88-89 4.5171580436037395 2315 90-91 4.424593723955443 2315 92-93 4.732594294554769 2315 94-95 4.991025801125485 2315 96-97 4.9533119912430585 2315 98-99 4.823300091552392 2315 100-101 4.589160619410023 2316 1 -0.7708880780584195 2316 2 -0.6903616505226999 2316 3 -0.6943623702938524 2316 4 -0.6684844823169414 2316 5 -0.5068513332805225 2316 6 -0.5602190469484043 2316 7 -0.5646436651045406 2316 8 -0.5091637083899059 2316 9 -0.517951324556762 2316 10-11 -0.561423242483194 2316 12-13 -0.44053255667343194 2316 14-15 -0.5393298484747078 2316 16-17 -0.4970677324491106 2316 18-19 -0.6202719303617528 2316 20-21 -0.6319921966172899 2316 22-23 -0.575309944670316 2316 24-25 -0.6104434217765657 2316 26-27 -0.4977758522183251 2316 28-29 -0.479406094941659 2316 30-31 -0.5397596097809867 2316 32-33 -0.47622185253948857 2316 34-35 -0.3783169747514279 2316 36-37 -0.09976924814334609 2316 38-39 -0.1869812245294824 2316 40-41 0.0010905362086361947 2316 42-43 0.29151275279107125 2316 44-45 0.38592987303081117 2316 46-47 0.4633552816981066 2316 48-49 0.5988868684498385 2316 50-51 0.5517086599826584 2316 52-53 0.6334697908157665 2316 54-55 1.0309330831517869 2316 56-57 1.2152858049013773 2316 58-59 1.3854073180357958 2316 60-61 1.3798557867555417 2316 62-63 1.7115394375268949 2316 64-65 2.0376405682354672 2316 66-67 2.277331406143947 2316 68-69 2.5094480538452615 2316 70-71 2.8205195574392654 2316 72-73 3.2682454445627336 2316 74-75 3.463176400051207 2316 76-77 3.9304063700386678 2316 78-79 4.227251584663254 2316 80-81 4.271912080680696 2316 82-83 4.466086456571592 2316 84-85 4.5940987340150095 2316 86-87 4.860689352801256 2316 88-89 5.1778263078194975 2316 90-91 5.227365374168354 2316 92-93 5.3937135694560645 2316 94-95 5.511309098622757 2316 96-97 5.668198664537471 2316 98-99 5.7405572257832365 2316 100-101 5.324147499923416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9159.0 3 3012.0 4 3165.0 5 3574.0 6 4312.0 7 5305.0 8 6891.0 9 9043.0 10 11556.0 11 14948.0 12 19214.0 13 24230.0 14 29208.0 15 34902.0 16 41450.0 17 51861.0 18 53372.0 19 49555.0 20 54139.0 21 59090.0 22 65774.0 23 74230.0 24 82367.0 25 89938.0 26 98456.0 27 107437.0 28 116820.0 29 126728.0 30 137841.0 31 151392.0 32 170210.0 33 195981.0 34 233568.0 35 288870.0 36 358874.0 37 406203.0 38 237360.0 39 16030.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75262368466205 18.95126604236755 14.928151807980687 23.36795846498972 2 15.138121963905892 19.79472404632975 40.89099203068835 24.176161959076005 3 18.704795150704363 24.14370442499098 30.02169571476972 27.129804709534938 4 12.596049310320304 16.85808550199363 37.67617930656092 32.86968588112514 5 13.530231945008225 37.20298396692689 34.578830582757405 14.687953505307483 6 31.624260440073183 36.62413123394178 17.47537606968885 14.276232256296186 7 28.11875991845495 32.95680335979996 20.824872923311023 18.099563798434062 8 25.788770309598185 35.26791804811895 19.830707630609286 19.11260401167357 9 26.085241321814088 16.952673835133773 20.591674305974454 36.37041053707769 10-11 24.635838964066114 26.61091449002651 27.555701983232257 21.197544562675116 12-13 25.57604950749977 24.051407659798784 27.917649862567863 22.45489297013358 14-15 22.392362246446254 25.536285758612376 26.396133869092996 25.67521812584837 16-17 21.753826549124675 28.77791356193451 27.12584288579714 22.342417003143677 18-19 21.690549789551707 27.235119677819124 29.593525150098888 21.48080538253029 20-21 22.292653592471616 26.391994483158232 29.893725898834578 21.42162602553557 22-23 22.032408138433247 26.275185882669838 29.660723491111067 22.031682487785847 24-25 21.94366290414408 26.381898897604785 29.739180219306128 21.935257978945007 26-27 21.593992575778646 26.814970137931894 29.618285752108108 21.97275153418135 28-29 21.62735423223335 26.990890476132424 29.646664147048185 21.73509114458604 30-31 21.797505531321075 26.586207832521918 29.561066466862222 22.055220169294785 32-33 21.587884936799714 26.650294175065188 29.47668262549159 22.285138262643503 34-35 22.238741218608443 26.76964244218662 29.191413108619507 21.80020323058543 36-37 22.27991909020606 26.358893807056617 29.21066504413578 22.150522058601542 38-39 21.801749454755637 26.756264414745896 29.208844589865514 22.23314154063295 40-41 22.260618561248833 26.49107959883226 29.213737008585632 22.03456483133327 42-43 22.066878025194693 26.495641165691424 29.108968245722284 22.328512563391595 44-45 21.981454158361707 26.802751926150957 29.060762705863368 22.155031209623964 46-47 22.16167119481223 26.991423853870295 28.550738981165797 22.29616597015168 48-49 22.16906699590111 26.868856783886784 28.60536242344878 22.356713796763323 50-51 22.354893115261447 27.199985373262585 28.27709657521038 22.16802493626559 52-53 22.426271344204807 27.077599817407755 28.204639706064793 22.291489132322642 54-55 22.17998392013522 27.159982756441703 28.359123944822358 22.30090937860072 56-57 22.23402080300354 27.226764869295252 28.294963681153334 22.24425064654788 58-59 22.297086023741127 26.85073527374 28.25712069040818 22.595058012110698 60-61 22.16934419133831 27.16670988258282 28.3728039506941 22.291141975384775 62-63 22.07222168293031 27.229835427938294 28.401337076357784 22.296605812773613 64-65 21.859591722546384 27.13133750885863 28.322761024019428 22.68630974457556 66-67 21.95564114473398 27.058779828948364 28.222664000904256 22.7629150254134 68-69 22.093675368053077 26.84273935584019 28.427942041317017 22.635643234789722 70-71 22.19082661736504 26.971323837255284 28.115627605332527 22.72222194004715 72-73 22.22801868068768 26.991004880005327 27.93016175311605 22.850814686190944 74-75 22.05247230442962 27.278821785561664 27.945590511133734 22.72311539887498 76-77 22.293829223830823 26.940611573838346 27.958991988686567 22.806567213644264 78-79 22.31195987517728 27.061503311087233 27.978302576986152 22.64823423674934 80-81 22.381250605812433 27.25970214052759 27.698498978172182 22.6605482754878 82-83 22.566169493679904 27.118242930967458 27.66651802796836 22.649069547384283 84-85 22.566066394392074 26.96609616883327 27.675676820786162 22.792160615988497 86-87 22.615346868727876 26.958520498025056 27.560586369646533 22.86554626360053 88-89 22.6604190183392 27.013072807926758 27.491834393103765 22.834673780630276 90-91 22.61692453014465 26.997263449145386 27.484435386300355 22.901376634409605 92-93 22.744089934859048 26.527097563417744 27.60251102491435 23.126301476808862 94-95 22.60457700502482 26.560819900031944 27.618407114477083 23.21619598046616 96-97 22.758774517814025 26.410756593640862 27.578022077533454 23.25244681101166 98-99 22.734018077644535 26.413442458229948 27.20427773154962 23.648261732575897 100-101 23.40575514613881 25.383507430188644 27.486004030513843 23.7247333931587 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1044.0 1 706.5 2 508.5 3 675.0 4 573.0 5 563.0 6 1059.0 7 2364.5 8 4016.5 9 4786.0 10 4066.0 11 3275.0 12 3325.5 13 3621.0 14 3970.5 15 4293.5 16 4585.0 17 4878.0 18 5292.5 19 5879.5 20 6686.5 21 7890.5 22 9393.0 23 11467.5 24 14456.0 25 18066.5 26 22630.5 27 27968.0 28 32808.5 29 38018.5 30 44405.0 31 51621.5 32 59039.5 33 64481.5 34 68932.0 35 73241.5 36 79036.0 37 85773.0 38 89966.5 39 93294.0 40 97642.5 41 103775.0 42 109350.5 43 115206.5 44 121544.5 45 126439.0 46 127737.5 47 127692.5 48 128127.0 49 124910.0 50 121274.0 51 117658.5 52 113761.0 53 111034.5 54 108642.5 55 102531.5 56 93910.0 57 86050.0 58 79273.5 59 71092.5 60 59600.0 61 50060.0 62 41864.0 63 33015.5 64 26299.0 65 21478.5 66 16730.5 67 13099.5 68 10039.5 69 7327.5 70 5567.0 71 4065.0 72 3016.5 73 2335.0 74 1794.5 75 1451.0 76 1028.5 77 601.0 78 332.0 79 206.5 80 143.0 81 75.5 82 34.5 83 26.0 84 17.5 85 12.5 86 10.5 87 8.0 88 6.5 89 4.5 90 6.0 91 7.0 92 5.5 93 2.5 94 1.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3831325343159417 2 0.024665807329285045 3 0.04663288515077773 4 0.039204124355134234 5 0.05977830952744376 6 0.05678939405107157 7 0.03189143794692267 8 0.029598968795142053 9 0.03377764674269152 10-11 0.030716184774174378 12-13 0.031717326365774776 14-15 0.02508657698372579 16-17 0.03064363828202942 18-19 0.026711618407772808 20-21 0.038928447684983394 22-23 0.025594402428740486 24-25 0.04808381499367685 26-27 0.04048094261688546 28-29 0.031920456543780645 30-31 0.039595875412716995 32-33 0.036664997130060765 34-35 0.0351270114965877 36-37 0.03604109729761415 38-39 0.017193518638354575 40-41 0.012332903664642523 42-43 0.012187810680352611 44-45 0.009692211350566124 46-47 0.01680176758077181 48-49 0.014422242638417257 50-51 0.009329478889841343 52-53 0.003961038471114599 54-55 0.00410613145540451 56-57 0.007704437465794329 58-59 0.0030324433716591615 60-61 0.0055280427014456485 62-63 0.005499024104587666 64-65 0.007893058345371214 66-67 0.004280243036552405 68-69 0.005731172879451525 70-71 0.017657816188082296 72-73 0.013798342805970632 74-75 0.009967888020716957 76-77 0.007298177109782576 78-79 0.006355072711898147 80-81 0.008125207120235074 82-83 0.010171018198722834 84-85 0.004570429005132229 86-87 4.207696544407449E-4 88-89 0.02388230521411952 90-91 0.0194569691932772 92-93 0.01337757315152989 94-95 0.025115595580583773 96-97 0.019239329716842335 98-99 0.017266065130499532 100-101 0.11099613298178271 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3446066.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.9469388287453 #Duplication Level Percentage of deduplicated Percentage of total 1 69.81522428534976 32.77611063839449 2 15.187644647589943 14.260268484462522 3 6.046659595355896 8.516164744242573 4 3.0075013052952273 5.6477191922826675 5 1.637736904883343 3.844336714556848 6 1.0267876492174692 2.8922722174754227 7 0.6655544590343406 2.1872021132838704 8 0.4898708735785507 1.8398350348700996 9 0.33550812051488904 1.4175971289323823 >10 1.612148253073874 13.191961159786192 >50 0.10310651467275267 3.3353590119741665 >100 0.06510081968395796 5.893713175381509 >500 0.004418405170572919 1.427560742181378 >1k 0.00255147340362269 1.7772711971699113 >5k 6.223105862494365E-5 0.2172470307588884 >10k+ 1.244621172498873E-4 0.7753814142470278 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14703 0.4266604296029153 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11837 0.34349313100793777 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7436 0.21578228623595716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 2.9018596857982406E-5 0.0 0.0 2.9018596857982406E-5 0.0 5 2.9018596857982406E-5 0.0 0.0 2.9018596857982406E-5 0.0 6 2.9018596857982406E-5 0.0 0.0 2.9018596857982406E-5 0.0 7 2.9018596857982406E-5 0.0 0.0 2.9018596857982406E-5 0.0 8 2.9018596857982406E-5 0.0 0.0 5.803719371596481E-5 0.0 9 2.9018596857982406E-5 0.0 0.0 5.803719371596481E-5 0.0 10-11 2.9018596857982406E-5 0.0 0.0 5.803719371596481E-5 0.0 12-13 2.9018596857982406E-5 0.0 0.0 5.803719371596481E-5 8.705579057394722E-5 14-15 2.9018596857982406E-5 0.0 0.0 5.803719371596481E-5 1.3058368586092083E-4 16-17 2.9018596857982406E-5 0.0 0.0 1.1607438743192964E-4 1.7411158114789444E-4 18-19 2.9018596857982406E-5 0.0 0.0 1.4509298428991204E-4 1.7411158114789444E-4 20-21 2.9018596857982406E-5 0.0 0.0 1.4509298428991204E-4 2.0313017800587685E-4 22-23 2.9018596857982406E-5 0.0 0.0 2.7567667015083287E-4 2.0313017800587685E-4 24-25 2.9018596857982406E-5 0.0 0.0 4.352789528697361E-4 2.0313017800587685E-4 26-27 2.9018596857982406E-5 0.0 0.0 8.705579057394722E-4 2.0313017800587685E-4 28-29 2.9018596857982406E-5 0.0 0.0 0.003351647937096968 2.0313017800587685E-4 30-31 2.9018596857982406E-5 0.0 0.0 0.012057226994491691 2.0313017800587685E-4 32-33 2.9018596857982406E-5 0.0 0.0 0.03077422196789034 2.0313017800587685E-4 34-35 2.9018596857982406E-5 0.0 0.0 0.05834188898297363 2.0313017800587685E-4 36-37 2.9018596857982406E-5 0.0 0.0 0.1031030746364115 2.0313017800587685E-4 38-39 2.9018596857982406E-5 0.0 0.0 0.1695701707396202 2.1763947643486806E-4 40-41 2.9018596857982406E-5 0.0 0.0 0.23774936405744987 2.3214877486385925E-4 42-43 2.9018596857982406E-5 0.0 0.0 0.31100681182542644 2.3214877486385925E-4 44-45 2.9018596857982406E-5 0.0 0.0 0.399136290483119 2.3214877486385925E-4 46-47 2.9018596857982406E-5 0.0 0.0 0.5030809044284119 2.3214877486385925E-4 48-49 2.9018596857982406E-5 0.0 0.0 0.6075913810124356 2.3214877486385925E-4 50-51 2.9018596857982406E-5 0.0 0.0 0.717687937491621 2.4665807329285044E-4 52-53 2.9018596857982406E-5 0.0 0.0 0.8273201964210783 2.6116737172184165E-4 54-55 2.9018596857982406E-5 0.0 0.0 0.9457450901985046 2.6116737172184165E-4 56-57 2.9018596857982406E-5 0.0 0.0 1.0816536885828651 2.6116737172184165E-4 58-59 2.9018596857982406E-5 0.0 0.0 1.2277623237628066 2.6116737172184165E-4 60-61 2.9018596857982406E-5 0.0 0.0 1.3774257370578509 2.6116737172184165E-4 62-63 2.9018596857982406E-5 0.0 0.0 1.5284675337036493 2.6116737172184165E-4 64-65 2.9018596857982406E-5 0.0 0.0 1.6868655446529464 2.7567667015083287E-4 66-67 2.9018596857982406E-5 0.0 0.0 1.8643432830363666 2.901859685798241E-4 68-69 2.9018596857982406E-5 0.0 0.0 2.0511650096080576 2.901859685798241E-4 70-71 2.9018596857982406E-5 0.0 0.0 2.2542516597186473 2.901859685798241E-4 72-73 2.9018596857982406E-5 0.0 0.0 2.461879720237511 3.0469526700881524E-4 74-75 2.9018596857982406E-5 0.0 0.0 2.6697399295312394 3.1920456543780646E-4 76-77 2.9018596857982406E-5 0.0 0.0 2.88949776353674 3.1920456543780646E-4 78-79 2.9018596857982406E-5 0.0 0.0 3.1289592248088107 3.1920456543780646E-4 80-81 2.9018596857982406E-5 0.0 0.0 3.3821174637978495 3.1920456543780646E-4 82-83 2.9018596857982406E-5 0.0 0.0 3.6402088642527453 3.7724175915377126E-4 84-85 2.9018596857982406E-5 0.0 0.0 3.9043941700478166 3.7724175915377126E-4 86-87 2.9018596857982406E-5 0.0 0.0 4.173062849057446 3.917510575827625E-4 88-89 2.9018596857982406E-5 0.0 0.0 4.455196157009181 4.207696544407449E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7890 0.0 46.407375 1 GTATCAA 12805 0.0 39.24315 1 ATCAACG 16060 0.0 31.130747 3 TATCAAC 16170 0.0 31.007147 2 TCAACGC 16250 0.0 30.766756 4 CAACGCA 16515 0.0 30.244299 5 AACGCAG 17050 0.0 29.261457 6 ACGCAGA 18940 0.0 26.184505 7 CGCAGAG 19235 0.0 25.68414 8 GTACATG 10505 0.0 23.872204 1 GCAGAGT 20615 0.0 23.388388 9 TACATGG 10320 0.0 23.347889 2 GTGGTAT 3805 0.0 22.929747 1 GAGTACT 13365 0.0 22.622505 12-13 ACATGGG 10250 0.0 22.487295 3 GGACCGA 1595 0.0 22.044594 6 GTACTTT 14035 0.0 21.42581 14-15 CAGAGTA 19970 0.0 20.682938 10-11 GTGTAGC 1810 0.0 20.5456 1 AGAGTAC 18685 0.0 20.516401 10-11 >>END_MODULE