FastQCFastQC Report
Thu 26 May 2016
SRR938191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3446066
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143820.41734546001150297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116220.33725413268347154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75060.21781358801601594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82850.044.490961
GTATCAA127450.037.3556941
ATCAACG159950.029.5224153
TATCAAC161550.029.4117642
TCAACGC162450.029.0969354
CAACGCA163950.028.8017165
AACGCAG169700.027.8474776
ACGCAGA191100.024.555057
CGCAGAG192550.024.2961398
GAGTACT132050.022.55141312-13
GTACATG105700.022.1387271
GCAGAGT208500.022.0502689
GTGGTAT42550.022.0206031
TACATGG101600.021.8617922
GTACTTT138600.021.245814-15
ACATGGG99550.020.9705623
AGTACTT138150.020.81650512-13
AGAGTAC187700.020.34400210-11
TGGTATC45100.020.1427782
CAGAGTA201900.020.05407510-11
Produced by FastQC (version 0.11.5)