##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938191_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3446066 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.520194621925405 34.0 31.0 34.0 31.0 34.0 2 32.7458661557846 34.0 31.0 34.0 31.0 34.0 3 32.81077176119088 34.0 31.0 34.0 31.0 34.0 4 36.25992943837988 37.0 35.0 37.0 35.0 37.0 5 36.18551298785339 37.0 37.0 37.0 35.0 37.0 6 36.237209618155894 37.0 37.0 37.0 35.0 37.0 7 36.20969998833452 37.0 37.0 37.0 35.0 37.0 8 36.20651838937501 37.0 37.0 37.0 35.0 37.0 9 37.90957718163262 39.0 38.0 39.0 35.0 39.0 10-11 37.882006902943814 39.0 38.0 39.0 35.0 39.0 12-13 37.80373504163879 39.0 38.0 39.0 35.0 39.0 14-15 39.07353254406619 40.0 38.0 41.0 36.0 41.0 16-17 38.99583699209475 40.0 38.0 41.0 36.0 41.0 18-19 39.23865314825659 40.5 39.0 41.0 36.0 41.0 20-21 39.20830825642922 41.0 39.0 41.0 36.0 41.0 22-23 39.10409609102089 40.0 39.0 41.0 35.5 41.0 24-25 39.007689057609454 40.0 38.5 41.0 35.0 41.0 26-27 38.85774372864594 40.0 38.0 41.0 35.0 41.0 28-29 38.73809468536006 40.0 38.0 41.0 35.0 41.0 30-31 38.51159945862906 40.0 38.0 41.0 34.0 41.0 32-33 38.29301586214541 40.0 38.0 41.0 34.0 41.0 34-35 38.1950257191824 40.0 38.0 41.0 34.0 41.0 36-37 38.1410238515455 40.0 38.0 41.0 34.0 41.0 38-39 37.96153962228233 40.0 38.0 41.0 33.0 41.0 40-41 37.756720561939325 40.0 37.0 41.0 33.0 41.0 42-43 37.512932572968715 40.0 37.0 41.0 33.0 41.0 44-45 37.24688499872028 40.0 37.0 41.0 32.0 41.0 46-47 36.889469035125856 40.0 36.0 41.0 31.0 41.0 48-49 36.49485804972975 39.0 36.0 41.0 30.0 41.0 50-51 36.1328780412215 39.0 35.0 40.5 29.5 41.0 52-53 35.627568943833346 39.0 35.0 40.0 28.0 41.0 54-55 35.270934886331254 38.0 34.5 40.0 27.0 41.0 56-57 34.906623523751435 38.0 34.0 40.0 26.0 41.0 58-59 34.43120300075506 38.0 34.0 40.0 25.5 41.0 60-61 33.98094174632755 37.0 33.0 40.0 24.5 41.0 62-63 33.44310831539501 36.5 32.5 39.5 22.5 41.0 64-65 32.90273735326021 36.0 32.0 39.0 21.5 40.0 66-67 32.36235739536039 35.5 31.0 39.0 19.0 40.0 68-69 31.75766947005658 35.0 31.0 38.0 16.0 40.0 70-71 31.159948329486433 35.0 30.0 37.5 10.5 40.0 72-73 30.502490375982354 34.0 29.0 37.0 7.5 39.0 74-75 29.850203971717313 34.0 29.0 36.0 6.5 39.0 76-77 28.516150735360263 32.5 27.0 35.0 2.0 37.0 78-79 29.175222848314572 34.0 29.0 35.0 2.0 37.5 80-81 28.89820885612754 34.0 29.0 35.0 2.0 37.0 82-83 28.336458878036577 34.0 27.5 35.0 2.0 36.5 84-85 27.68265378550498 33.0 26.0 35.0 2.0 36.0 86-87 26.970733729417834 33.0 25.5 35.0 2.0 36.0 88-89 26.180214627346082 32.0 24.0 35.0 2.0 35.0 90-91 25.52701457255897 32.0 21.5 34.0 2.0 35.0 92-93 24.59021243354016 31.0 17.5 34.0 2.0 35.0 94-95 23.694888316126274 31.0 4.5 34.0 2.0 35.0 96-97 22.644889128646984 30.5 2.0 34.0 2.0 35.0 98-99 21.21962289753011 29.5 2.0 34.0 2.0 35.0 100-101 17.276237164349144 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1741315239100132 1101 2 0.11238309074531116 1101 3 0.07681660661395284 1101 4 0.01573220899432215 1101 5 -0.056786536123361486 1101 6 0.026327659775198242 1101 7 0.021073452981063667 1101 8 0.10250965471166751 1101 9 0.020261963117121695 1101 10-11 0.14145211384209944 1101 12-13 0.19772807012277127 1101 14-15 0.27949761961822617 1101 16-17 0.33882406172347856 1101 18-19 0.22504711093581165 1101 20-21 0.19908182828978482 1101 22-23 0.2857013385942153 1101 24-25 0.23392363094307456 1101 26-27 0.22456983557484023 1101 28-29 0.28047771320358805 1101 30-31 0.3319393451755417 1101 32-33 0.30262438866026287 1101 34-35 0.2843187895145931 1101 36-37 0.3965134426269401 1101 38-39 0.5050973814863298 1101 40-41 0.49085562257456417 1101 42-43 0.5308630750998802 1101 44-45 0.4938115227449771 1101 46-47 0.5075267614795465 1101 48-49 0.6148500317135941 1101 50-51 0.6700487713119188 1101 52-53 0.7821525136059222 1101 54-55 1.0851984553620966 1101 56-57 0.9578530524739293 1101 58-59 1.0837545406591502 1101 60-61 1.124172795341991 1101 62-63 1.0030235373842729 1101 64-65 1.3096119285979384 1101 66-67 1.3655193355152093 1101 68-69 1.499445895995109 1101 70-71 1.5596840841859922 1101 72-73 1.6951540473250155 1101 74-75 1.707401041343605 1101 76-77 1.78450831737997 1101 78-79 1.9938822457627374 1101 80-81 2.098638294159759 1101 82-83 2.1888480375425097 1101 84-85 2.3845044377942095 1101 86-87 2.5937575621242104 1101 88-89 2.986982272318638 1101 90-91 3.0934240799756374 1101 92-93 3.3869110643102154 1101 94-95 3.7460478346931936 1101 96-97 3.9573719524107993 1101 98-99 4.177159451599437 1101 100-101 4.197730809057774 1102 1 0.1898260132794647 1102 2 0.15913899132497278 1102 3 0.16832090144527 1102 4 0.12425666617179587 1102 5 -0.09151690902664456 1102 6 -0.017839533155914467 1102 7 0.07221095432880986 1102 8 0.11001700957820049 1102 9 0.17396814839300845 1102 10-11 0.20616107676740825 1102 12-13 0.2479568026212675 1102 14-15 0.34770816647116476 1102 16-17 0.34495799474156996 1102 18-19 0.24332774081018016 1102 20-21 0.20900281160848522 1102 22-23 0.27450140431309933 1102 24-25 0.3460687875131683 1102 26-27 0.31165350905470746 1102 28-29 0.3168284671736714 1102 30-31 0.3589734214402469 1102 32-33 0.350672589349287 1102 34-35 0.4439828017547356 1102 36-37 0.41365477415300944 1102 38-39 0.4740123525546167 1102 40-41 0.5387395260705006 1102 42-43 0.6275612149475975 1102 44-45 0.5501351676782065 1102 46-47 0.5881992504279907 1102 48-49 0.8038831940751621 1102 50-51 0.6701216781913502 1102 52-53 0.7540345892955926 1102 54-55 0.8447217881306628 1102 56-57 1.0118287878322292 1102 58-59 1.0349182268720512 1102 60-61 1.0642720924580615 1102 62-63 1.173776583300203 1102 64-65 1.1741144931744358 1102 66-67 1.259839786921745 1102 68-69 1.4611492471444194 1102 70-71 1.464908221497371 1102 72-73 1.7059370774786764 1102 74-75 2.0148611607671256 1102 76-77 2.0383936892820635 1102 78-79 1.985082590786842 1102 80-81 2.163374468807678 1102 82-83 2.246646763212407 1102 84-85 2.4067708148870715 1102 86-87 2.481092302252261 1102 88-89 2.684936772264731 1102 90-91 2.8161920769361366 1102 92-93 3.0256097278552367 1102 94-95 3.352043655383344 1102 96-97 3.692875035550312 1102 98-99 4.080594289792373 1102 100-101 3.7394032517976363 1103 1 0.19360547256214744 1103 2 0.19274778808073023 1103 3 0.18668986321012682 1103 4 0.08670510082843919 1103 5 -0.010395109655028989 1103 6 0.038158130165491855 1103 7 0.1203022095888926 1103 8 0.05742280684336265 1103 9 0.11492279778632764 1103 10-11 0.20707831993851755 1103 12-13 0.2153917653360793 1103 14-15 0.3267136687467911 1103 16-17 0.30981464994538754 1103 18-19 0.265148843965612 1103 20-21 0.25619452091792283 1103 22-23 0.3586968182048267 1103 24-25 0.37439138686369944 1103 26-27 0.35053564718016617 1103 28-29 0.2588512433350374 1103 30-31 0.29565723128065713 1103 32-33 0.29720127148730313 1103 34-35 0.34043329875616024 1103 36-37 0.37091270549317557 1103 38-39 0.34209189214717384 1103 40-41 0.4446017169003298 1103 42-43 0.45791194803747004 1103 44-45 0.4690388114229407 1103 46-47 0.5409659607191202 1103 48-49 0.7113306040143286 1103 50-51 0.8147331435158236 1103 52-53 0.908152635673062 1103 54-55 0.8943113363351429 1103 56-57 0.9703399405775244 1103 58-59 1.0922944982882399 1103 60-61 1.087100592406152 1103 62-63 1.3223445339028999 1103 64-65 1.3659172374808008 1103 66-67 1.5337543529175832 1103 68-69 1.6385488207973964 1103 70-71 1.6606924100798075 1103 72-73 1.8765787872991275 1103 74-75 1.9679055937003085 1103 76-77 1.9137947680056513 1103 78-79 1.9598561981527602 1103 80-81 2.142437634974897 1103 82-83 2.314141810411268 1103 84-85 2.486123322540177 1103 86-87 2.63467281572985 1103 88-89 2.8105685334501516 1103 90-91 3.0630232255502925 1103 92-93 3.1082534881864596 1103 94-95 3.5057804853431804 1103 96-97 3.8828274251860897 1103 98-99 4.1463412926954675 1103 100-101 4.058085276854534 1104 1 0.2115927589862494 1104 2 0.16776903956669997 1104 3 0.20763308473446784 1104 4 0.09939540568116456 1104 5 0.027913436087231958 1104 6 0.10079045218916605 1104 7 0.1233354414591119 1104 8 0.15156883745944327 1104 9 0.13685724274426292 1104 10-11 0.25065683438891284 1104 12-13 0.3092283525832116 1104 14-15 0.36884122851031975 1104 16-17 0.3416873169463841 1104 18-19 0.30543450576332987 1104 20-21 0.3320265991184712 1104 22-23 0.40484457887433933 1104 24-25 0.34395850034806585 1104 26-27 0.4544277855860841 1104 28-29 0.3803587927895862 1104 30-31 0.4046394234990629 1104 32-33 0.42700950988776043 1104 34-35 0.5385985871346222 1104 36-37 0.5877535701016185 1104 38-39 0.63978861242677 1104 40-41 0.6763175965273192 1104 42-43 0.7342449790180936 1104 44-45 0.8594444472391061 1104 46-47 0.8014233218114839 1104 48-49 0.8302896154029966 1104 50-51 0.9873612808522907 1104 52-53 1.0076587462185245 1104 54-55 1.076812598302304 1104 56-57 1.0300874733343406 1104 58-59 1.144483862662753 1104 60-61 1.1868326986139621 1104 62-63 1.4158971016523978 1104 64-65 1.5259743468768505 1104 66-67 1.5635521039711406 1104 68-69 1.6614263068522064 1104 70-71 1.5607418431069782 1104 72-73 1.8029181434928994 1104 74-75 1.9519505767298746 1104 76-77 1.885942711973076 1104 78-79 1.913311599556728 1104 80-81 2.1601642242026386 1104 82-83 2.377605329001245 1104 84-85 2.411571715566982 1104 86-87 2.6329492207780447 1104 88-89 2.689413073858322 1104 90-91 2.981266462698013 1104 92-93 3.274484087619836 1104 94-95 3.496700113925023 1104 96-97 3.8754615760882487 1104 98-99 4.318911867516896 1104 100-101 3.84598606675781 1105 1 0.26510551651179526 1105 2 0.23219052847159816 1105 3 0.20621438287437854 1105 4 0.14827611573011978 1105 5 0.0650649922941895 1105 6 0.08941808830307707 1105 7 0.16772283407699717 1105 8 0.1666560944777089 1105 9 0.23628070128526701 1105 10-11 0.22864906745072489 1105 12-13 0.32708209281135225 1105 14-15 0.3676506972296636 1105 16-17 0.3221963288579204 1105 18-19 0.30276091432535424 1105 20-21 0.29539306887338057 1105 22-23 0.3716460512210844 1105 24-25 0.3664027273699162 1105 26-27 0.3785387077918685 1105 28-29 0.45180262743971156 1105 30-31 0.48118875714764897 1105 32-33 0.6065527293036723 1105 34-35 0.5702454187425943 1105 36-37 0.5640581963937947 1105 38-39 0.6422513380842645 1105 40-41 0.6891116274216316 1105 42-43 0.6750906139895037 1105 44-45 0.8220029384317442 1105 46-47 0.7156515511105752 1105 48-49 0.8195609317071089 1105 50-51 0.8047298839583661 1105 52-53 0.8768675688661034 1105 54-55 0.9228188451402914 1105 56-57 0.8870129510926219 1105 58-59 1.0050463619579233 1105 60-61 1.0984866446331054 1105 62-63 1.2195102853426434 1105 64-65 1.1703028837347134 1105 66-67 1.3678018223306765 1105 68-69 1.6087861778400594 1105 70-71 1.6872752135646074 1105 72-73 1.8082352860156128 1105 74-75 1.7362848533367803 1105 76-77 1.691709954260876 1105 78-79 1.7642074728871542 1105 80-81 1.9302905803447317 1105 82-83 2.005800446248063 1105 84-85 2.1383915484998433 1105 86-87 2.338354948316514 1105 88-89 2.5042148308592793 1105 90-91 2.721829173928363 1105 92-93 3.0314839760136003 1105 94-95 3.1703578059470416 1105 96-97 3.494768929150087 1105 98-99 3.8110968064309567 1105 100-101 3.9733639211260687 1106 1 0.13318595219400464 1106 2 0.08135435336370023 1106 3 0.04601307606377958 1106 4 0.057596859688622715 1106 5 -0.013752851615855377 1106 6 0.005990059886073595 1106 7 0.09164764378321877 1106 8 0.1281371403392626 1106 9 0.13043759969763613 1106 10-11 0.11987309222966758 1106 12-13 0.07806435836771897 1106 14-15 0.042904695814854676 1106 16-17 -0.012485602619349834 1106 18-19 -0.017632893667318683 1106 20-21 -0.04104371833173559 1106 22-23 0.017467628348789788 1106 24-25 0.04140117831693857 1106 26-27 -0.11414510578476467 1106 28-29 0.00380276832976989 1106 30-31 0.037013675803919455 1106 32-33 0.049279077293256535 1106 34-35 -0.048395534382329686 1106 36-37 0.1156164883637274 1106 38-39 0.03450589801675363 1106 40-41 0.015081288481468391 1106 42-43 0.06531817355980962 1106 44-45 0.13442534747466794 1106 46-47 0.22236356077075925 1106 48-49 0.25488552653754937 1106 50-51 0.4921113998468556 1106 52-53 0.5555970706537892 1106 54-55 0.5703660488414926 1106 56-57 0.5710572259772349 1106 58-59 0.7049198375238248 1106 60-61 0.7298391910180229 1106 62-63 0.684597627957011 1106 64-65 0.8896850218261463 1106 66-67 1.107661380393239 1106 68-69 0.8459652749192159 1106 70-71 0.8852974325166123 1106 72-73 1.285315664161569 1106 74-75 1.3571928178295352 1106 76-77 1.4168058848987073 1106 78-79 1.6956172973499513 1106 80-81 1.4638267935150786 1106 82-83 1.963783352448477 1106 84-85 2.093987161212997 1106 86-87 2.2283785031422916 1106 88-89 2.3036796883795994 1106 90-91 2.419744757180002 1106 92-93 2.325095810630806 1106 94-95 2.5710717165165278 1106 96-97 2.9803408837672656 1106 98-99 3.350636614141848 1106 100-101 3.4437203950979196 1107 1 0.23075748475829272 1107 2 0.19675492260748229 1107 3 0.22561752377270494 1107 4 0.14988392338315748 1107 5 0.05463564633691931 1107 6 0.1194013363343771 1107 7 0.12717920030645757 1107 8 0.1869253877723125 1107 9 0.1315612222216771 1107 10-11 0.233930615582004 1107 12-13 0.21974326683619694 1107 14-15 0.37500906701251324 1107 16-17 0.334985372287143 1107 18-19 0.24411308785193597 1107 20-21 0.2725710234208947 1107 22-23 0.265137860440241 1107 24-25 0.3303571602952573 1107 26-27 0.3700620134788082 1107 28-29 0.34515311558158857 1107 30-31 0.37116240057979866 1107 32-33 0.44833121206675486 1107 34-35 0.3877282193038738 1107 36-37 0.4223345596678527 1107 38-39 0.3723081721500492 1107 40-41 0.4520744013749294 1107 42-43 0.48646375481586546 1107 44-45 0.5968063997886972 1107 46-47 0.6053619933829282 1107 48-49 0.659649636853672 1107 50-51 0.7164559442660234 1107 52-53 0.771489853237199 1107 54-55 0.6719607074810412 1107 56-57 0.7552324017891863 1107 58-59 0.9202200677134229 1107 60-61 0.9593556234509251 1107 62-63 1.0470747838804115 1107 64-65 1.0756306135751714 1107 66-67 1.208577411469932 1107 68-69 1.4815735613074281 1107 70-71 1.4227626745435948 1107 72-73 1.604396315775194 1107 74-75 1.5549398617623034 1107 76-77 1.6292823030349375 1107 78-79 1.673969665592093 1107 80-81 1.8280601561412944 1107 82-83 2.013721296350422 1107 84-85 2.008892839223005 1107 86-87 2.1562531132081126 1107 88-89 2.343734699976519 1107 90-91 2.5689518272577594 1107 92-93 2.6691841680214665 1107 94-95 2.723055052363801 1107 96-97 2.976661511859138 1107 98-99 3.231749180788622 1107 100-101 3.336258688984234 1108 1 0.24148322457254778 1108 2 0.23268333694951338 1108 3 0.17573689289432082 1108 4 0.13623357613398923 1108 5 0.05769214393120592 1108 6 0.13452493077327432 1108 7 0.15492957202921076 1108 8 0.13573097172878335 1108 9 0.26412447620204915 1108 10-11 0.1890888141329654 1108 12-13 0.23130014205133875 1108 14-15 0.3556806210054759 1108 16-17 0.28516869676621326 1108 18-19 0.3494590735446934 1108 20-21 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2316 38-39 -0.32939201149895325 2316 40-41 -0.38015068544859076 2316 42-43 -0.39115803292191487 2316 44-45 -0.2557945200480205 2316 46-47 -0.45832152777944657 2316 48-49 -0.20762811049996088 2316 50-51 -0.2194702786517766 2316 52-53 -0.15688176647471863 2316 54-55 0.09532847625680319 2316 56-57 0.09254433077192203 2316 58-59 0.2629851832651653 2316 60-61 0.4946449254771679 2316 62-63 0.4769346172612714 2316 64-65 0.6262161838714988 2316 66-67 0.8354107288144519 2316 68-69 1.1011576759771344 2316 70-71 1.0431292369173555 2316 72-73 1.1697990637561944 2316 74-75 1.5488710703195672 2316 76-77 1.6772776867870682 2316 78-79 1.6208262807059732 2316 80-81 1.7687810132445314 2316 82-83 1.8131101439647708 2316 84-85 2.0967686783305908 2316 86-87 2.259477668170817 2316 88-89 2.5353923361943345 2316 90-91 2.7432909697616132 2316 92-93 2.814637023342563 2316 94-95 3.3058113905630364 2316 96-97 3.619988250347806 2316 98-99 3.926842402696469 2316 100-101 4.361158601210711 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 25.0 5 71.0 6 119.0 7 293.0 8 771.0 9 1467.0 10 2587.0 11 4460.0 12 6782.0 13 10463.0 14 14156.0 15 18612.0 16 24712.0 17 34911.0 18 36016.0 19 28966.0 20 27610.0 21 28946.0 22 31245.0 23 35691.0 24 40303.0 25 46221.0 26 52803.0 27 62073.0 28 74401.0 29 89682.0 30 107945.0 31 130013.0 32 158757.0 33 199503.0 34 261135.0 35 357597.0 36 504964.0 37 653842.0 38 381375.0 39 17540.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.04054710501772 19.364283794912808 15.288186587256309 23.306982512813164 2 15.33906249546514 20.086982263976804 40.467881574097454 24.106073666460603 3 18.716457117054684 24.10458966175483 30.296126306674303 26.88282691451618 4 12.811080154276663 17.178818328522468 37.40672202139456 32.603379495806315 5 13.463105651199877 37.25733786135119 34.762928398417536 14.516628089031391 6 31.658563707137354 36.84218468247561 17.307967984362456 14.191283626024573 7 28.146239799237737 32.897860923151214 20.81477835885906 18.14112091875199 8 25.767921357052813 35.499336052577206 19.724926118438006 19.00781647193197 9 25.90324735509999 16.929565481334368 20.582107249251756 36.58507991431389 10-11 24.64317282373582 26.737967293719855 27.470817448069766 21.148042434474558 12-13 25.499787293685035 24.020985088503817 27.966368026613537 22.512859591197614 14-15 22.296761335499863 25.560831620873138 26.390502444019127 25.751904599607872 16-17 21.761995203224547 28.83259601893754 27.163634464236747 22.241774313601166 18-19 21.650165725206655 27.285548216429984 29.64330921114105 21.420976847222313 20-21 22.197035692293763 26.3968681969527 29.997394130002153 21.408701980751385 22-23 22.03254967417825 26.280173624572555 29.740535266983475 21.946741434265725 24-25 21.957614956600317 26.471797206686322 29.747358606613574 21.82322923009979 26-27 21.523037886226547 26.922900010490856 29.651481424139398 21.902580679143195 28-29 21.55996141687362 27.038716031555982 29.693018067558775 21.708304484011624 30-31 21.843951915024263 26.60482997133543 29.570588607414948 21.98062950622536 32-33 21.574673845480614 26.66616077579478 29.54194725231612 22.21721812640849 34-35 22.183705544326564 26.808507489131 29.258493246715066 21.749293719827374 36-37 22.235751437047824 26.43716752603459 29.278370349971222 22.048710686946364 38-39 21.73782513501509 26.76188545624211 29.310815412941803 22.189473995801 40-41 22.245247609820144 26.52753921647868 29.259266622137915 21.96794655156327 42-43 22.0332709251373 26.5124555934706 29.141029028589806 22.313244452802298 44-45 21.934198767621652 26.814480293249577 29.16717753378361 22.084143405345163 46-47 22.191989676342484 26.998804868915 28.549123630993083 22.260081823749434 48-49 22.164767012587685 26.895610821150846 28.647405476273523 22.292216689987946 50-51 22.312761856563398 27.16427950016047 28.363792219882033 22.159166423394097 52-53 22.444535304895496 27.086727300057518 28.244424221706723 22.224313173340267 54-55 22.137605605928616 27.18689949641127 28.36210914126427 22.313385756395842 56-57 22.19337772020485 27.333154578672943 28.270818533840348 22.202649167281862 58-59 22.32899776150544 26.95772512772535 28.183557714797104 22.5297193959721 60-61 22.118250201824342 27.29573374392713 28.320119231610768 22.265896822637757 62-63 21.975348121597207 27.403189608092244 28.305943647045645 22.315518623264904 64-65 21.783651270753378 27.26614928443042 28.257627683277104 22.6925717615391 66-67 21.82723852883152 27.199107039089675 28.233490183799574 22.740164248279232 68-69 21.968656354980542 27.063395207029085 28.340504154713887 22.62744428327649 70-71 22.000840958936944 27.198463407259176 27.989394283220342 22.811301350583534 72-73 22.093988968283814 27.251162485664803 27.771350154843322 22.883498391208057 74-75 21.87617706683505 27.561689184130543 27.779401207057553 22.782732541976852 76-77 21.98264629870699 27.20934538108092 27.985607356330377 22.822400963881716 78-79 21.99947708488462 27.345268488763708 28.05301755683147 22.6022368695202 80-81 22.036668849353543 27.611849885891377 27.774775152540215 22.57670611221487 82-83 22.182225716777634 27.488994109249415 27.752778551219816 22.576001622753132 84-85 22.114719669359033 27.406684382339307 27.716399108160882 22.76219684014077 86-87 22.17577823583337 27.40998390292188 27.59726079791246 22.816977063332295 88-89 22.0926557993956 27.69130074699672 27.380482556050872 22.83556089755681 90-91 22.082153451310894 27.531031937053353 27.568597600788863 22.818217010846887 92-93 22.08332632050576 27.171360037793818 27.6481500934689 23.09716354823152 94-95 21.923045281640757 27.270520540018754 27.592539956074543 23.213894222265942 96-97 21.999736510987606 27.11416561038913 27.610659581481944 23.275438297141317 98-99 21.917281909284384 27.210491615656814 27.33910203693139 23.533124438127416 100-101 22.13481982063025 26.765767109509802 27.474749467944026 23.624663601915923 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1015.0 1 683.5 2 473.0 3 627.0 4 549.5 5 539.5 6 1053.0 7 2465.5 8 4117.0 9 4725.5 10 4040.5 11 3231.5 12 3337.5 13 3740.5 14 4038.5 15 4372.0 16 4571.5 17 4797.5 18 5184.0 19 5703.0 20 6395.5 21 7516.0 22 8957.5 23 10912.0 24 13762.5 25 17329.5 26 22173.5 27 27702.0 28 32491.0 29 37818.0 30 44304.5 31 51328.5 32 59234.5 33 65116.5 34 69356.5 35 73697.5 36 79977.0 37 87113.0 38 91273.0 39 94525.5 40 98332.0 41 105173.0 42 111376.0 43 117968.0 44 125367.5 45 130352.0 46 131445.0 47 131607.5 48 131848.0 49 127274.0 50 123490.5 51 119914.5 52 115331.0 53 112256.0 54 110590.0 55 103857.5 56 93671.0 57 85297.5 58 78132.5 59 69389.0 60 56873.0 61 46721.5 62 38734.5 63 29446.0 64 22542.0 65 17985.5 66 13272.0 67 10447.0 68 7927.5 69 5437.5 70 4049.0 71 2846.5 72 2092.0 73 1597.0 74 1245.5 75 1046.5 76 681.5 77 352.0 78 170.5 79 87.5 80 44.5 81 21.5 82 12.5 83 6.0 84 3.0 85 3.0 86 4.0 87 2.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01555396791587857 3 0.00301793407323017 4 0.0014219112460411379 5 0.11865704255229007 6 0.0 7 0.0 8 2.901859685798241E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.4509298428991205E-5 16-17 2.901859685798241E-5 18-19 0.0 20-21 0.0 22-23 2.901859685798241E-4 24-25 4.497882512987273E-4 26-27 0.006079396041747314 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010809427329598446 36-37 7.399742198785514E-4 38-39 0.0071821027223506455 40-41 0.010229055392438798 42-43 0.005934303057457402 44-45 0.0036998710993927566 46-47 1.4509298428991205E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.803719371596482E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.254649214495602E-5 68-69 0.0011752531727482875 70-71 0.0 72-73 5.803719371596482E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1763947643486803E-4 82-83 0.0020458110784877595 84-85 0.004439845319271309 86-87 0.0028728410889402585 88-89 0.0 90-91 0.002901859685798241 92-93 0.0 94-95 1.4509298428991205E-5 96-97 5.803719371596482E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3446066.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.931699975931764 #Duplication Level Percentage of deduplicated Percentage of total 1 68.13392610911895 30.61373126117236 2 15.86049054411034 14.25277605198137 3 6.287068066185142 8.47465968234277 4 3.2038819538011603 5.758234508259832 5 1.8376213389828207 4.128372533627306 6 1.0982395238565483 2.960746127257955 7 0.7526943502028296 2.367388570182472 8 0.5027090321766187 1.8070057127160688 9 0.3702679644383142 1.4973092179957177 >10 1.756026710025586 13.780237111333049 >50 0.1157739489750428 3.60478603682398 >100 0.07303480165581686 6.218949751321209 >500 0.005359761621207292 1.681149046861818 >1k 0.002712168526331962 1.8823669839605168 >5k 6.457544110314194E-5 0.21778541497021456 >10k+ 1.2915088220628388E-4 0.7545019891933733 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14382 0.41734546001150297 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11622 0.33725413268347154 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7506 0.21781358801601594 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.9018596857982406E-5 0.0 5 0.0 0.0 0.0 2.9018596857982406E-5 0.0 6 0.0 0.0 0.0 2.9018596857982406E-5 0.0 7 0.0 0.0 0.0 2.9018596857982406E-5 0.0 8 0.0 0.0 0.0 2.9018596857982406E-5 0.0 9 0.0 0.0 0.0 2.9018596857982406E-5 0.0 10-11 0.0 0.0 0.0 5.803719371596481E-5 1.4509298428991203E-5 12-13 0.0 0.0 0.0 5.803719371596481E-5 5.803719371596481E-5 14-15 0.0 0.0 0.0 5.803719371596481E-5 7.254649214495602E-5 16-17 0.0 0.0 0.0 1.1607438743192964E-4 8.705579057394722E-5 18-19 0.0 0.0 0.0 1.4509298428991204E-4 8.705579057394722E-5 20-21 0.0 0.0 0.0 1.4509298428991204E-4 8.705579057394722E-5 22-23 0.0 0.0 0.0 2.7567667015083287E-4 8.705579057394722E-5 24-25 0.0 0.0 0.0 4.352789528697361E-4 8.705579057394722E-5 26-27 0.0 0.0 0.0 8.705579057394722E-4 1.1607438743192962E-4 28-29 0.0 0.0 0.0 0.0034241944292419243 1.1607438743192962E-4 30-31 0.0 0.0 0.0 0.012245847874068575 1.1607438743192962E-4 32-33 0.0 0.0 0.0 0.03112244513018613 1.1607438743192962E-4 34-35 0.0 0.0 0.0 0.05883520512955934 1.1607438743192962E-4 36-37 0.0 0.0 0.0 0.10416225342172786 1.1607438743192962E-4 38-39 0.0 2.9018596857982406E-5 0.0 0.17091953549351638 1.1607438743192962E-4 40-41 0.0 2.9018596857982406E-5 0.0 0.23962106355478974 1.1607438743192962E-4 42-43 0.0 2.9018596857982406E-5 0.0 0.31315418799291717 1.7411158114789444E-4 44-45 0.0 2.9018596857982406E-5 0.0 0.4015883619176185 2.1763947643486806E-4 46-47 0.0 2.9018596857982406E-5 0.0 0.506330987276506 2.3214877486385925E-4 48-49 0.0 2.9018596857982406E-5 0.0 0.611944170541133 2.3214877486385925E-4 50-51 0.0 2.9018596857982406E-5 0.0 0.7224179687794721 2.3214877486385925E-4 52-53 0.0 2.9018596857982406E-5 0.0 0.8330803878973879 2.3214877486385925E-4 54-55 0.0 2.9018596857982406E-5 0.0 0.9524193674758406 2.3214877486385925E-4 56-57 0.0 2.9018596857982406E-5 0.0 1.0892565609596567 2.3214877486385925E-4 58-59 0.0 2.9018596857982406E-5 0.0 1.2364388842233434 2.6116737172184165E-4 60-61 0.0 5.803719371596481E-5 0.0 1.3873500971832808 2.6116737172184165E-4 62-63 0.0 5.803719371596481E-5 0.0 1.5393640168238218 2.6116737172184165E-4 64-65 0.0 5.803719371596481E-5 0.0 1.6996337272704585 2.6116737172184165E-4 66-67 0.0 5.803719371596481E-5 0.0 1.8788235628685 2.7567667015083287E-4 68-69 0.0 5.803719371596481E-5 0.0 2.068315000351125 2.901859685798241E-4 70-71 0.0 5.803719371596481E-5 0.0 2.274985447173676 2.901859685798241E-4 72-73 0.0 5.803719371596481E-5 0.0 2.4868357135353762 3.7724175915377126E-4 74-75 0.0 5.803719371596481E-5 0.0 2.7009784490488578 4.642975497277185E-4 76-77 0.0 5.803719371596481E-5 0.0 2.9290936389494573 4.642975497277185E-4 78-79 0.0 5.803719371596481E-5 0.0 3.1780586906925175 4.642975497277185E-4 80-81 0.0 5.803719371596481E-5 0.0 3.442476145262453 4.642975497277185E-4 82-83 0.0 5.803719371596481E-5 0.0 3.7142498141358873 4.933161465857009E-4 84-85 0.0 5.803719371596481E-5 0.0 3.9930024555536665 4.933161465857009E-4 86-87 0.0 5.803719371596481E-5 0.0 4.279532080929384 5.223347434436833E-4 88-89 0.0 5.803719371596481E-5 0.0 4.58264583440944 5.513533403016657E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8285 0.0 44.49096 1 GTATCAA 12745 0.0 37.355694 1 ATCAACG 15995 0.0 29.522415 3 TATCAAC 16155 0.0 29.411764 2 TCAACGC 16245 0.0 29.096935 4 CAACGCA 16395 0.0 28.801716 5 AACGCAG 16970 0.0 27.847477 6 ACGCAGA 19110 0.0 24.55505 7 CGCAGAG 19255 0.0 24.296139 8 GAGTACT 13205 0.0 22.551413 12-13 GTACATG 10570 0.0 22.138727 1 GCAGAGT 20850 0.0 22.050268 9 GTGGTAT 4255 0.0 22.020603 1 TACATGG 10160 0.0 21.861792 2 GTACTTT 13860 0.0 21.2458 14-15 ACATGGG 9955 0.0 20.970562 3 AGTACTT 13815 0.0 20.816505 12-13 AGAGTAC 18770 0.0 20.344002 10-11 TGGTATC 4510 0.0 20.142778 2 CAGAGTA 20190 0.0 20.054075 10-11 >>END_MODULE