FastQCFastQC Report
Thu 26 May 2016
SRR938190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3411399
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144780.42440066377459806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116460.3413848687884355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73600.21574726380584622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69800.049.5698551
GTATCAA119700.040.7067681
ATCAACG147050.032.77283
TCAACGC149200.032.300544
TATCAAC149950.032.1389852
CAACGCA151300.031.7893945
AACGCAG157150.030.6322126
ACGCAGA175600.027.2417727
CGCAGAG177050.026.8845168
GCAGAGT192000.024.270529
GTACATG98700.023.7893731
TACATGG96750.023.1863142
ACATGGG96550.022.5451873
GAGTACT128350.022.4285312-13
GTGTAGC19450.022.328361
GTACTTT131900.021.57388714-15
GTGGTAT33200.021.5619581
CAGAGTA186950.021.15228810-11
AGAGTAC175300.020.91866510-11
AGTACTT131950.020.7365812-13
Produced by FastQC (version 0.11.5)