##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938190_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3411399 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85398102068975 33.0 31.0 34.0 30.0 34.0 2 32.05525533659358 34.0 31.0 34.0 30.0 34.0 3 32.13458994389106 34.0 31.0 34.0 30.0 34.0 4 35.656579895814005 37.0 35.0 37.0 33.0 37.0 5 35.66610648593143 37.0 35.0 37.0 35.0 37.0 6 35.68948633683718 37.0 35.0 37.0 35.0 37.0 7 35.632635760284856 37.0 35.0 37.0 33.0 37.0 8 35.60567174933217 37.0 35.0 37.0 33.0 37.0 9 37.24643203565458 39.0 37.0 39.0 34.0 39.0 10-11 37.15588970976424 39.0 37.0 39.0 34.0 39.0 12-13 37.05032495465936 39.0 37.0 39.0 33.0 39.0 14-15 38.24029129984502 40.0 38.0 41.0 33.0 41.0 16-17 38.09040044861361 40.0 38.0 41.0 33.0 41.0 18-19 38.24266539915149 40.0 38.0 41.0 33.5 41.0 20-21 38.221526857456425 40.0 38.0 41.0 34.0 41.0 22-23 38.130784027315485 40.0 38.0 41.0 33.0 41.0 24-25 38.031783294771444 40.0 38.0 41.0 33.0 41.0 26-27 37.85637871735321 40.0 38.0 41.0 33.0 41.0 28-29 37.64878793128567 40.0 38.0 41.0 32.0 41.0 30-31 37.436878242621276 40.0 37.0 41.0 32.0 41.0 32-33 37.183880425596655 40.0 36.5 41.0 31.0 41.0 34-35 36.947062187683116 40.0 36.0 41.0 30.5 41.0 36-37 36.6836670234118 39.0 36.0 41.0 30.0 41.0 38-39 36.394424545472404 39.0 35.5 41.0 30.0 41.0 40-41 36.187348211100485 39.0 35.0 40.0 30.0 41.0 42-43 36.00942384634574 39.0 35.0 40.5 29.0 41.0 44-45 35.735350804757815 39.0 35.0 40.0 27.5 41.0 46-47 35.355301300141086 39.0 35.0 40.0 26.5 41.0 48-49 34.97252314959347 38.0 34.5 40.0 24.5 41.0 50-51 34.28650386542295 37.5 33.5 39.5 24.0 40.5 52-53 34.41374330589884 38.0 34.0 40.0 24.0 40.5 54-55 34.51941827971457 38.0 34.0 40.0 23.5 41.0 56-57 34.22261541379358 38.0 34.0 40.0 23.0 41.0 58-59 33.912243481340056 38.0 33.5 40.0 22.0 41.0 60-61 33.50332502882249 37.5 33.0 40.0 20.5 41.0 62-63 32.96375079549475 37.0 32.5 39.5 17.5 41.0 64-65 32.45285453270051 36.0 31.5 39.0 13.5 41.0 66-67 31.920186410326085 35.5 31.0 39.0 8.0 40.0 68-69 31.315712410069885 35.0 30.5 38.5 6.5 40.0 70-71 30.759174754990546 35.0 30.0 38.0 2.0 40.0 72-73 30.106938971372156 34.5 29.0 37.0 2.0 39.0 74-75 29.404078356123104 34.0 28.5 36.5 2.0 39.0 76-77 28.712764909645575 34.0 27.5 36.0 2.0 38.5 78-79 27.968900735446073 33.5 26.0 35.0 2.0 37.5 80-81 27.292296357007785 33.0 25.5 35.0 2.0 37.0 82-83 26.541058082036137 32.5 24.5 35.0 2.0 36.5 84-85 25.850908527557166 32.0 21.5 35.0 2.0 36.0 86-87 25.1385890363455 31.5 19.0 34.5 2.0 35.5 88-89 24.300485226149156 31.0 13.5 34.0 2.0 35.0 90-91 23.45292283312506 30.5 4.5 34.0 2.0 35.0 92-93 22.52342543337792 30.0 2.0 34.0 2.0 35.0 94-95 21.546761167485833 29.0 2.0 34.0 2.0 35.0 96-97 20.314864664027866 28.5 2.0 34.0 2.0 35.0 98-99 18.784300517177847 26.0 2.0 33.5 2.0 35.0 100-101 15.115807620275435 10.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3516937786248917 1101 2 0.4026168177334668 1101 3 0.2463820027032284 1101 4 0.23407443193644895 1101 5 0.30461794707273526 1101 6 0.3045552963084859 1101 7 0.2962215693079955 1101 8 0.2712985504915224 1101 9 0.3156543542884904 1101 10-11 0.41856855731163733 1101 12-13 0.45838547109209316 1101 14-15 0.5856812701311611 1101 16-17 0.6959281883831281 1101 18-19 0.5511042126747583 1101 20-21 0.6631198246827168 1101 22-23 0.5852322691699001 1101 24-25 0.6013088880771207 1101 26-27 0.6335361660273549 1101 28-29 0.7600981608537367 1101 30-31 0.7649723989635149 1101 32-33 0.7206935308904008 1101 34-35 0.83181271728958 1101 36-37 0.9496611444863703 1101 38-39 0.9168497494090175 1101 40-41 1.0759074731198126 1101 42-43 1.2793213991334227 1101 44-45 1.1987134795454395 1101 46-47 1.1976201248689904 1101 48-49 1.2108024242269337 1101 50-51 1.1969433159677365 1101 52-53 1.2042441730927607 1101 54-55 1.2668191330823149 1101 56-57 1.424469285837091 1101 58-59 1.5171580432362433 1101 60-61 1.4380851933901297 1101 62-63 1.3878633326611691 1101 64-65 1.446534536955518 1101 66-67 1.6188635504108362 1101 68-69 1.8559729253714288 1101 70-71 2.071330837199696 1101 72-73 2.2397120501716756 1101 74-75 2.2848597757087212 1101 76-77 2.4551342496099906 1101 78-79 2.5724172316842946 1101 80-81 2.8084774686435914 1101 82-83 3.1471376646490867 1101 84-85 3.111885196149899 1101 86-87 3.298501849467627 1101 88-89 3.7300358299421212 1101 90-91 4.032202426181662 1101 92-93 4.260492445436828 1101 94-95 4.571061023592232 1101 96-97 4.831007393793712 1101 98-99 5.321078425654687 1101 100-101 5.410652939819839 1102 1 0.36249650416526435 1102 2 0.2897520405615879 1102 3 0.22416616951417723 1102 4 0.19526740915354424 1102 5 0.21056382490206005 1102 6 0.32397866312070533 1102 7 0.13549749770776032 1102 8 0.19362615646254966 1102 9 0.3538401936141895 1102 10-11 0.36108637291884804 1102 12-13 0.3726740672503084 1102 14-15 0.49360400659995207 1102 16-17 0.5535480370265731 1102 18-19 0.4598370503108953 1102 20-21 0.39302875978091123 1102 22-23 0.4907608125285847 1102 24-25 0.39776016843406836 1102 26-27 0.45735615837109833 1102 28-29 0.5193870826408542 1102 30-31 0.4722793526495579 1102 32-33 0.5548093795931521 1102 34-35 0.41550485131313764 1102 36-37 0.6391174924761884 1102 38-39 0.5386691625039646 1102 40-41 0.7538019831224432 1102 42-43 0.9336520252463245 1102 44-45 0.784809231385502 1102 46-47 0.6998528801713135 1102 48-49 1.0555706796978939 1102 50-51 1.138818663106683 1102 52-53 0.9648304745467584 1102 54-55 1.0430562714025982 1102 56-57 1.1078304432333965 1102 58-59 1.102939391106915 1102 60-61 1.273729485909719 1102 62-63 1.3179145384676332 1102 64-65 1.4470666622321033 1102 66-67 1.4435396026025344 1102 68-69 1.655276820793194 1102 70-71 1.7426129096608562 1102 72-73 2.037725978616976 1102 74-75 1.9345823028219549 1102 76-77 2.1109265523781566 1102 78-79 2.408180815571857 1102 80-81 2.427740297760426 1102 82-83 2.711784743810796 1102 84-85 2.8599270545537436 1102 86-87 2.930690875191253 1102 88-89 3.266801027148805 1102 90-91 3.4055584105790118 1102 92-93 3.639935678259377 1102 94-95 3.957813169169004 1102 96-97 4.183345435773898 1102 98-99 4.38316320247711 1102 100-101 4.169332856141152 1103 1 0.5514399475390874 1103 2 0.46465300913344265 1103 3 0.47394755473860073 1103 4 0.35777394128033535 1103 5 0.3878191160814026 1103 6 0.46040443372368856 1103 7 0.4456342306293024 1103 8 0.4673556656350968 1103 9 0.5969521050805326 1103 10-11 0.5925366993058887 1103 12-13 0.5891286356036147 1103 14-15 0.7636439449943211 1103 16-17 0.7577354279228246 1103 18-19 0.7797531132649311 1103 20-21 0.7912815333501371 1103 22-23 0.8262461448493568 1103 24-25 0.8087162838205657 1103 26-27 0.7645095421067651 1103 28-29 0.8464674945313888 1103 30-31 0.9089626488265239 1103 32-33 0.8946150637026378 1103 34-35 0.8958005107227223 1103 36-37 0.9458694245445685 1103 38-39 0.9689572402603943 1103 40-41 0.9699912562737367 1103 42-43 1.0376222425076804 1103 44-45 0.8493896276114796 1103 46-47 1.0855042569619329 1103 48-49 1.1899114330259906 1103 50-51 1.2014914903364726 1103 52-53 1.2064392136238524 1103 54-55 1.2199888723858265 1103 56-57 1.3333382573488066 1103 58-59 1.3346566418115131 1103 60-61 1.4577514338557194 1103 62-63 1.4692656391858208 1103 64-65 1.5670038410300364 1103 66-67 1.731284267562387 1103 68-69 1.889810561656052 1103 70-71 1.9500704367386419 1103 72-73 2.0752710769215668 1103 74-75 2.1734052543031375 1103 76-77 2.280758277273172 1103 78-79 2.470580577971621 1103 80-81 2.499822332500692 1103 82-83 2.7845198032547174 1103 84-85 2.8855324276274743 1103 86-87 2.9193673315892283 1103 88-89 3.3096099857805505 1103 90-91 3.3609029747843344 1103 92-93 3.5074836337820123 1103 94-95 4.048640747338354 1103 96-97 4.398058166309411 1103 98-99 4.7768091505534365 1103 100-101 4.396208187446861 1104 1 0.29083925468032845 1104 2 0.2001564849594999 1104 3 0.2505543458615236 1104 4 0.23717823036204067 1104 5 0.28695903941487444 1104 6 0.2200959224474346 1104 7 0.21118918650684293 1104 8 0.27276683717530403 1104 9 0.34272483633713335 1104 10-11 0.3163146246967088 1104 12-13 0.2368493978405013 1104 14-15 0.4840854694680985 1104 16-17 0.5082036380684656 1104 18-19 0.455413912312828 1104 20-21 0.4762524489662141 1104 22-23 0.47235077482495313 1104 24-25 0.5218340098415197 1104 26-27 0.5295953652571086 1104 28-29 0.5472467299880392 1104 30-31 0.649384752958305 1104 32-33 0.5748190003581826 1104 34-35 0.5655361449744802 1104 36-37 0.7136218543937787 1104 38-39 0.8192195993937261 1104 40-41 0.873908422287677 1104 42-43 1.0063491491378187 1104 44-45 1.0307375654110231 1104 46-47 0.9547380068408486 1104 48-49 1.0412491762992104 1104 50-51 1.0700630572951368 1104 52-53 0.9993212661833155 1104 54-55 1.1622047057306943 1104 56-57 1.3513810697464521 1104 58-59 1.3418804554869368 1104 60-61 1.3730086265322612 1104 62-63 1.4806993078775577 1104 64-65 1.460814933228228 1104 66-67 1.6231668183159087 1104 68-69 1.8205777343587108 1104 70-71 2.0725027769129127 1104 72-73 1.9735027187015959 1104 74-75 2.131970270932669 1104 76-77 2.25208190504841 1104 78-79 2.5628311234479746 1104 80-81 2.5292156659262837 1104 82-83 2.8578749556421243 1104 84-85 3.0733995574300863 1104 86-87 2.9689515932702655 1104 88-89 3.2505316678312646 1104 90-91 3.538608675877132 1104 92-93 3.7899182573456685 1104 94-95 4.006117233040644 1104 96-97 4.122882068552666 1104 98-99 4.364315896931149 1104 100-101 4.185891303742883 1105 1 0.3491115567547496 1105 2 0.25640052036336414 1105 3 0.17493733694385583 1105 4 0.08564648212175996 1105 5 0.12495088083748129 1105 6 0.15780894994181693 1105 7 0.20140142972305597 1105 8 0.23074472661066636 1105 9 0.29136156459612295 1105 10-11 0.23117076393587865 1105 12-13 0.2590590611897454 1105 14-15 0.3582881634245183 1105 16-17 0.44660436373855106 1105 18-19 0.2946596868513538 1105 20-21 0.2916580345553683 1105 22-23 0.29646931738425053 1105 24-25 0.22589146381924508 1105 26-27 0.3374441744918215 1105 28-29 0.45966687344753865 1105 30-31 0.3815680461484092 1105 32-33 0.5106885833236916 1105 34-35 0.5133324883448438 1105 36-37 0.5980766186534794 1105 38-39 0.6990135105463864 1105 40-41 0.7314173689647561 1105 42-43 0.6671567888930525 1105 44-45 0.7308743233735768 1105 46-47 0.8391182082205475 1105 48-49 0.8887319126257651 1105 50-51 0.9450952361501592 1105 52-53 0.9695653356333693 1105 54-55 1.0747465295569754 1105 56-57 1.2544700400682558 1105 58-59 1.120773419495606 1105 60-61 1.236371475822665 1105 62-63 1.1509562401843567 1105 64-65 1.1632974906386053 1105 66-67 1.4459620326036315 1105 68-69 1.528444626286042 1105 70-71 1.686976817218735 1105 72-73 1.7883573988185901 1105 74-75 1.8961452085638832 1105 76-77 1.8508622178772605 1105 78-79 2.076185825972914 1105 80-81 2.2396137439844566 1105 82-83 2.523981249913021 1105 84-85 2.6895812049388255 1105 86-87 2.7950237527676016 1105 88-89 3.2327858326696877 1105 90-91 3.2189365910034695 1105 92-93 3.559782946345937 1105 94-95 3.856357927402147 1105 96-97 3.9664394188284966 1105 98-99 4.119601176160234 1105 100-101 4.086018516614564 1106 1 0.1722975832903373 1106 2 0.1355969013539351 1106 3 0.07720065586404701 1106 4 0.10441815388199416 1106 5 0.17082248419115587 1106 6 0.006383239276964048 1106 7 0.05122036387501083 1106 8 0.09293686993970596 1106 9 0.07576587325600315 1106 10-11 0.07717483288018911 1106 12-13 0.22408715034936222 1106 14-15 0.34707448799932905 1106 16-17 0.1826922996882132 1106 18-19 0.1722734451469634 1106 20-21 0.16105890951049417 1106 22-23 0.17135417080844917 1106 24-25 0.09542046520559921 1106 26-27 0.20498025598173797 1106 28-29 0.18604278410667519 1106 30-31 0.2528220097071596 1106 32-33 0.2723074977780513 1106 34-35 0.23583994225151628 1106 36-37 0.3908953014853438 1106 38-39 0.19862946567729978 1106 40-41 0.5208004241041806 1106 42-43 0.39644093962424876 1106 44-45 0.3981779295912631 1106 46-47 0.7318814852540569 1106 48-49 0.6881493365830451 1106 50-51 0.7210881386565191 1106 52-53 0.8337784069204872 1106 54-55 0.4017435754171217 1106 56-57 0.7872937325808707 1106 58-59 0.8094368931875096 1106 60-61 0.9467393579500296 1106 62-63 0.9134248439833215 1106 64-65 0.980751479245761 1106 66-67 1.2546638781760464 1106 68-69 1.048111719957209 1106 70-71 1.173515766566947 1106 72-73 1.409317737563974 1106 74-75 1.3585978937510959 1106 76-77 1.7171092178794325 1106 78-79 1.9630310831880813 1106 80-81 1.9464131482404419 1106 82-83 1.9905618635520952 1106 84-85 2.3165395745920634 1106 86-87 2.1486370268163704 1106 88-89 2.545743231488263 1106 90-91 2.721615206773162 1106 92-93 3.0449353712337484 1106 94-95 3.204990866454427 1106 96-97 3.3770485548742677 1106 98-99 3.7019179980027097 1106 100-101 3.624192250537307 1107 1 0.2690892436056487 1107 2 0.14432836003181393 1107 3 -7.713099083161978E-4 1107 4 0.037410831708591274 1107 5 0.14787278980414698 1107 6 0.11048402552989955 1107 7 0.03771671551754707 1107 8 0.13517087589634968 1107 9 0.12091105493875887 1107 10-11 0.10437664819618675 1107 12-13 0.10274278734726039 1107 14-15 0.24139798174115867 1107 16-17 0.2958155667223963 1107 18-19 0.22550280397864952 1107 20-21 0.32189190101086496 1107 22-23 0.29800654466730947 1107 24-25 0.38081481527161287 1107 26-27 0.40120147160445185 1107 28-29 0.5050686444994383 1107 30-31 0.5535858751044245 1107 32-33 0.47699023276845054 1107 34-35 0.527158079850075 1107 36-37 0.5156378925144836 1107 38-39 0.5516858183127127 1107 40-41 0.5943435712735337 1107 42-43 0.7036516049399211 1107 44-45 0.6681257524627071 1107 46-47 0.7514063793388743 1107 48-49 0.836058655432204 1107 50-51 0.691693792409275 1107 52-53 0.6439603935910725 1107 54-55 0.7819695224959773 1107 56-57 0.8454314043340432 1107 58-59 0.8375871159650075 1107 60-61 0.9729827415330661 1107 62-63 1.0973400730156513 1107 64-65 1.170785253084837 1107 66-67 1.3031724416088366 1107 68-69 1.4312575941102743 1107 70-71 1.6169920820775516 1107 72-73 1.6762578016492995 1107 74-75 1.7990323202179717 1107 76-77 2.0446792581006257 1107 78-79 2.2808980264185656 1107 80-81 2.3910189445606136 1107 82-83 2.5401065070428572 1107 84-85 2.624373335892841 1107 86-87 2.8559458027000737 1107 88-89 3.085903486174768 1107 90-91 3.35972341353283 1107 92-93 3.6171171268687417 1107 94-95 3.8867130872092552 1107 96-97 4.282608381794876 1107 98-99 4.3476327174201685 1107 100-101 4.054296744815995 1108 1 0.16913539314972326 1108 2 0.11180857537028288 1108 3 0.1345573393766557 1108 4 0.1280917525875438 1108 5 0.1169076190214966 1108 6 0.05747462549352633 1108 7 0.06492245839810096 1108 8 0.126940222039444 1108 9 0.15289699106075005 1108 10-11 0.16822874672938326 1108 12-13 0.1484816787022396 1108 14-15 0.25750632366235493 1108 16-17 0.2985312522875603 1108 18-19 0.2952399471263263 1108 20-21 0.2735531868232002 1108 22-23 0.17410039641385566 1108 24-25 0.2689999453409726 1108 26-27 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12026.0 12 15062.0 13 18891.0 14 22347.0 15 26661.0 16 31862.0 17 39336.0 18 38347.0 19 31916.0 20 32824.0 21 34722.0 22 38106.0 23 42721.0 24 47842.0 25 54180.0 26 61728.0 27 70677.0 28 82125.0 29 96073.0 30 112388.0 31 134745.0 32 164589.0 33 206729.0 34 268844.0 35 361081.0 36 474897.0 37 538807.0 38 286652.0 39 15957.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.80017152406079 18.923604055083256 14.912314803197935 23.363909617658024 2 15.15670042023337 19.83460459060237 40.8880619118419 24.120633077322353 3 18.71449001115672 24.13459158306947 29.99964218718655 27.151276218587263 4 12.595179721428925 16.889423608530475 37.625485009258036 32.889911660782566 5 13.507883816210049 37.179898285642196 34.6442564539623 14.667961444185456 6 31.617423142256136 36.658041348718086 17.49091261144703 14.23362289757875 7 28.137141389115683 32.943782435691695 20.869376249331346 18.049699925861276 8 25.79424266080309 35.28867341666134 19.81657946180298 19.100504460732594 9 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26.685272369036326 29.499193008330337 22.242485197087937 34-35 22.224522828647086 26.73756477420933 29.23779279819599 21.8001195989476 36-37 22.23816111774621 26.416409877423003 29.218077669490068 22.127351335340727 38-39 21.760284415774812 26.739935493329426 29.263788300835653 22.23599179006011 40-41 22.21756186152366 26.528055061909168 29.232903325875125 22.02147975069204 42-43 22.004830259225475 26.50869367495235 29.12411162549042 22.362364440331756 44-45 21.964699101220575 26.81894649072606 29.07495438084605 22.14140002720731 46-47 22.15888224114749 27.026726676668332 28.540919235586465 22.27347184659771 48-49 22.1737907843987 26.878661190921743 28.63066694737385 22.316881077305702 50-51 22.29084703166426 27.158375837812464 28.31786343272008 22.232913697803195 52-53 22.422392707959986 27.11275879786299 28.176938688901643 22.287909805275383 54-55 22.112149584770975 27.177619748398374 28.3807878349791 22.329442831851555 56-57 22.157748184285577 27.27610952996286 28.340082633421755 22.22605965232981 58-59 22.256281198570413 26.886756289773984 28.25559780393141 22.60136470772419 60-61 22.081181179640424 27.191553955748276 28.405731453730805 22.321533410880498 62-63 21.972833392703482 27.28172674779121 28.430575773167917 22.31486408633739 64-65 21.794140326084726 27.169334197263375 28.365676958575335 22.670848518076564 66-67 21.87762380858569 27.099838408570676 28.286811301549776 22.73572648129386 68-69 21.996476363210466 26.900587795622027 28.466024785658135 22.636911055509373 70-71 22.038437497068124 27.0113557469485 28.19460276581572 22.755603990167657 72-73 22.066456797950664 27.054916461745382 28.022956070495365 22.85567066980859 74-75 21.90001907087461 27.32228942551519 28.068003132841906 22.70968837076829 76-77 22.176252673461107 27.04663882304535 28.02032028324213 22.75678822025142 78-79 22.165322484888765 27.15794460831582 28.07744527574026 22.59928763105516 80-81 22.226893719001552 27.36672270996851 27.796568824707606 22.609814746322332 82-83 22.342013238645233 27.266170651135823 27.768010736334592 22.623805373884352 84-85 22.327031256298717 27.127726085028563 27.777302994433306 22.76793966423941 86-87 22.403707924983173 27.12872674485414 27.664726636977 22.80283869318568 88-89 22.419319227069064 27.20021744950149 27.58060746875703 22.799855854672423 90-91 22.44408032283017 27.20535325237557 27.537182531467558 22.8133838933267 92-93 22.555195867985816 26.710093040390014 27.710479896318507 23.02423119530566 94-95 22.380640581249384 26.73527530789732 27.72451118065841 23.159572930194887 96-97 22.548652949744284 26.56416970201649 27.677684332903535 23.209493015335696 98-99 22.575031277386955 26.66601380221902 27.347548577073137 23.41140634332089 100-101 23.23864356942559 25.622484112035398 27.51766405820655 23.62120826033246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1077.0 1 723.5 2 515.0 3 689.0 4 570.5 5 550.0 6 1020.0 7 2316.0 8 4017.0 9 4637.5 10 3886.0 11 3214.5 12 3277.5 13 3580.5 14 3905.5 15 4194.0 16 4442.5 17 4725.5 18 5149.0 19 5683.5 20 6470.5 21 7567.5 22 8952.5 23 11072.0 24 13847.5 25 17300.5 26 21879.5 27 27231.5 28 32056.5 29 37053.0 30 43384.5 31 50409.0 32 58431.5 33 64331.0 34 68619.0 35 72955.5 36 78677.5 37 85765.0 38 90331.0 39 93528.0 40 97396.0 41 104121.5 42 109699.5 43 115644.0 44 122704.0 45 127959.5 46 128940.0 47 128367.5 48 128717.5 49 125125.5 50 121574.5 51 117631.0 52 113359.5 53 110878.5 54 108737.0 55 102228.5 56 93013.0 57 84961.5 58 77943.0 59 69557.0 60 57368.0 61 47321.0 62 39336.0 63 30461.0 64 23940.5 65 19397.0 66 14665.5 67 11264.5 68 8650.5 69 6164.5 70 4622.5 71 3359.5 72 2426.5 73 1905.5 74 1473.0 75 1159.5 76 759.5 77 405.5 78 236.0 79 149.0 80 92.5 81 52.0 82 29.5 83 23.5 84 14.0 85 12.0 86 11.5 87 8.0 88 5.5 89 5.5 90 4.5 91 4.0 92 4.0 93 3.5 94 2.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4677553109442783 2 0.040716433345967445 3 0.05261770903960516 4 0.05651640280131407 5 0.08307442196002285 6 0.08292785452537214 7 0.04616874191497389 8 0.04165446492773199 9 0.04622736888883417 10-11 0.049759644063916295 12-13 0.04514276987241891 14-15 0.039749088277272755 16-17 0.039338699460250766 18-19 0.0473266246487145 20-21 0.055534400989154305 22-23 0.04703348977941308 24-25 0.0642991335812668 26-27 0.05544646052836388 28-29 0.04355984157819123 30-31 0.04511345638548877 32-33 0.044336648981840004 34-35 0.0489535231733374 36-37 0.044014200625608436 38-39 0.02635282475019779 40-41 0.02456470204745912 42-43 0.02391980533499599 44-45 0.016151731298508325 46-47 0.02836079860491253 48-49 0.028287514887587174 50-51 0.017060449393342732 52-53 0.016312955476624105 54-55 0.016723344293646098 56-57 0.016166388041973397 58-59 0.014231697904584013 60-61 0.01675265778057624 62-63 0.017412211236504438 64-65 0.01843818327905941 66-67 0.009585510226156483 68-69 0.012736710071146766 70-71 0.018027794462037422 72-73 0.015726685738021263 74-75 0.012853964018867334 76-77 0.009512226508831127 78-79 0.01541889412525477 80-81 0.018863228839546473 82-83 0.016166388041973397 84-85 0.014261011391514155 86-87 0.003253797049245778 88-89 0.027906439557495326 90-91 0.031101609632880823 92-93 0.01666471731978581 94-95 0.03195170075385494 96-97 0.026132973598221726 98-99 0.023099027700952016 100-101 0.14794516853642745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3411399.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.89766928825306 #Duplication Level Percentage of deduplicated Percentage of total 1 69.74748183585065 32.709943368261605 2 15.272101640504992 14.324519441459804 3 5.965773585682943 8.393426300098628 4 3.0000108623322737 5.627740691313034 5 1.6840256156511957 3.948843819787826 6 1.0472884646334706 2.946923283826969 7 0.7002356616233298 2.29875943378564 8 0.4734113900075724 1.7761512644693853 9 0.34998651009280796 1.4772196445114116 >10 1.5829511058888708 13.035797865354692 >50 0.10368500788105596 3.3501826038400684 >100 0.06588200375411522 5.929023647687493 >500 0.004588956801219227 1.5099289267877336 >1k 0.0023887720300020676 1.6843975464657748 >5k 6.286242184215967E-5 0.21698023876762235 >10k+ 1.2572484368431934E-4 0.7701619235822508 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14478 0.42440066377459806 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11646 0.3413848687884355 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7360 0.21574726380584622 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.931348693014215E-5 2 0.0 0.0 0.0 0.0 2.931348693014215E-5 3 0.0 0.0 0.0 0.0 2.931348693014215E-5 4 0.0 0.0 0.0 0.0 2.931348693014215E-5 5 0.0 0.0 0.0 0.0 2.931348693014215E-5 6 0.0 0.0 0.0 0.0 2.931348693014215E-5 7 0.0 0.0 0.0 0.0 2.931348693014215E-5 8 0.0 0.0 0.0 0.0 2.931348693014215E-5 9 0.0 0.0 0.0 0.0 2.931348693014215E-5 10-11 0.0 0.0 0.0 2.931348693014215E-5 2.931348693014215E-5 12-13 0.0 0.0 0.0 4.397023039521323E-5 1.0259720425549753E-4 14-15 0.0 0.0 0.0 8.794046079042645E-5 1.172539477205686E-4 16-17 0.0 0.0 0.0 8.794046079042645E-5 1.172539477205686E-4 18-19 0.0 0.0 0.0 1.172539477205686E-4 1.172539477205686E-4 20-21 0.0 0.0 0.0 1.6122417811578182E-4 1.172539477205686E-4 22-23 0.0 0.0 0.0 2.784781258363504E-4 1.172539477205686E-4 24-25 0.0 0.0 0.0 6.888669428583404E-4 1.172539477205686E-4 26-27 0.0 0.0 0.0 0.0012165097076008993 1.172539477205686E-4 28-29 0.0 0.0 0.0 0.004045261196359617 1.4656743465071075E-4 30-31 0.0 0.0 0.0 0.013132442144703684 1.4656743465071075E-4 32-33 0.0 0.0 0.0 0.03230346259701665 1.4656743465071075E-4 34-35 0.0 0.0 0.0 0.061191903966671735 1.4656743465071075E-4 36-37 0.0 0.0 0.0 0.1082840207199451 1.4656743465071075E-4 38-39 0.0 0.0 0.0 0.1745178444386013 1.4656743465071075E-4 40-41 0.0 0.0 0.0 0.24689284366912226 1.4656743465071075E-4 42-43 0.0 0.0 0.0 0.32005930704675706 1.4656743465071075E-4 44-45 0.0 0.0 0.0 0.40940681520983035 1.90537665045924E-4 46-47 0.0 0.0 0.0 0.51657692342643 2.345078954411372E-4 48-49 0.0 0.0 0.0 0.624406585098958 2.345078954411372E-4 50-51 0.0 0.0 0.0 0.736809150732588 2.6382138237127935E-4 52-53 0.0 0.0 0.0 0.8481124606063377 2.931348693014215E-4 54-55 0.0 0.0 0.0 0.9692944155755454 2.931348693014215E-4 56-57 0.0 0.0 0.0 1.1060858023350537 3.0779161276649257E-4 58-59 0.0 0.0 0.0 1.2493114994757284 3.2244835623156364E-4 60-61 0.0 0.0 0.0 1.4017856017428627 3.371050996966347E-4 62-63 0.0 0.0 0.0 1.5545381821358335 3.517618431617058E-4 64-65 0.0 0.0 0.0 1.714692417978665 3.517618431617058E-4 66-67 0.0 0.0 0.0 1.8978723978051233 3.517618431617058E-4 68-69 0.0 0.0 0.0 2.0958996587616987 3.517618431617058E-4 70-71 0.0 0.0 0.0 2.3012552914508095 3.517618431617058E-4 72-73 0.0 0.0 0.0 2.508208509177613 3.517618431617058E-4 74-75 0.0 0.0 0.0 2.722665979558533 3.517618431617058E-4 76-77 0.0 0.0 0.0 2.950783534848899 3.517618431617058E-4 78-79 0.0 0.0 0.0 3.2013845346146845 3.517618431617058E-4 80-81 0.0 0.0 0.0 3.46236250875374 3.517618431617058E-4 82-83 0.0 0.0 0.0 3.734010592135367 3.517618431617058E-4 84-85 1.4656743465071076E-5 0.0 0.0 4.008707278157729 3.517618431617058E-4 86-87 2.931348693014215E-5 0.0 0.0 4.287757016989218 3.517618431617058E-4 88-89 2.931348693014215E-5 0.0 0.0 4.586754583676667 3.517618431617058E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6980 0.0 49.569855 1 GTATCAA 11970 0.0 40.706768 1 ATCAACG 14705 0.0 32.7728 3 TCAACGC 14920 0.0 32.30054 4 TATCAAC 14995 0.0 32.138985 2 CAACGCA 15130 0.0 31.789394 5 AACGCAG 15715 0.0 30.632212 6 ACGCAGA 17560 0.0 27.241772 7 CGCAGAG 17705 0.0 26.884516 8 GCAGAGT 19200 0.0 24.27052 9 GTACATG 9870 0.0 23.789373 1 TACATGG 9675 0.0 23.186314 2 ACATGGG 9655 0.0 22.545187 3 GAGTACT 12835 0.0 22.42853 12-13 GTGTAGC 1945 0.0 22.32836 1 GTACTTT 13190 0.0 21.573887 14-15 GTGGTAT 3320 0.0 21.561958 1 CAGAGTA 18695 0.0 21.152288 10-11 AGAGTAC 17530 0.0 20.918665 10-11 AGTACTT 13195 0.0 20.73658 12-13 >>END_MODULE