FastQCFastQC Report
Thu 26 May 2016
SRR938190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3411399
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142990.4191535496141026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116830.3424694678048507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77180.22624149212683708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70950.049.213051
GTATCAA120650.041.382881
ATCAACG148200.033.3664253
TCAACGC151650.032.885884
TATCAAC152650.032.489952
CAACGCA154100.032.301395
AACGCAG158850.031.4551016
ACGCAGA178450.027.7606987
CGCAGAG178950.027.4973378
GCAGAGT194700.024.931459
GAGTACT129550.023.13420112-13
GTACATG97700.022.561541
GTGGTAT35400.022.1424671
GTACTTT136100.022.00338714-15
ACATGGG92300.021.6149753
CAGAGTA190150.021.40656910-11
TACATGG98600.021.3922
TAAGGTG16200.021.1096045
AGAGTAC178900.020.8677110-11
GGACCGA15750.020.8080396
Produced by FastQC (version 0.11.5)