##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938190_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3411399 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.531925758317925 34.0 31.0 34.0 31.0 34.0 2 32.76062606572846 34.0 31.0 34.0 31.0 34.0 3 32.72293155975012 34.0 31.0 34.0 31.0 34.0 4 36.240214938211565 37.0 35.0 37.0 35.0 37.0 5 36.20427894831417 37.0 37.0 37.0 35.0 37.0 6 36.26178321562502 37.0 37.0 37.0 35.0 37.0 7 36.2253471376406 37.0 37.0 37.0 35.0 37.0 8 36.22271947667218 37.0 37.0 37.0 35.0 37.0 9 37.93921789858061 39.0 38.0 39.0 35.0 39.0 10-11 37.90222764912577 39.0 38.0 39.0 35.0 39.0 12-13 37.847392081665035 39.0 38.0 39.0 35.0 39.0 14-15 39.14912811430149 40.0 38.5 41.0 36.0 41.0 16-17 39.09218886445122 40.0 38.0 41.0 36.0 41.0 18-19 39.08751981225298 40.0 38.5 41.0 36.0 41.0 20-21 39.09346341486294 40.0 39.0 41.0 36.0 41.0 22-23 39.11631796808289 40.0 39.0 41.0 36.0 41.0 24-25 39.07613606617109 40.0 39.0 41.0 35.5 41.0 26-27 38.91477074361574 40.0 38.0 41.0 35.0 41.0 28-29 38.840400375329885 40.0 38.0 41.0 35.0 41.0 30-31 38.636709015861236 40.0 38.0 41.0 35.0 41.0 32-33 38.443237070773606 40.0 38.0 41.0 34.0 41.0 34-35 38.242639749850426 40.0 38.0 41.0 34.0 41.0 36-37 38.16383923428482 40.0 38.0 41.0 34.0 41.0 38-39 38.11835291034558 40.0 38.0 41.0 34.0 41.0 40-41 38.004007886500524 40.0 38.0 41.0 33.5 41.0 42-43 37.82544404802839 40.0 38.0 41.0 33.0 41.0 44-45 37.583770910409484 40.0 37.0 41.0 33.0 41.0 46-47 37.24312342238478 40.0 37.0 41.0 32.0 41.0 48-49 36.892257106248785 40.0 36.5 41.0 31.0 41.0 50-51 36.60809612126872 40.0 36.0 41.0 30.5 41.0 52-53 36.16288933074085 39.0 35.0 41.0 30.0 41.0 54-55 35.86428793582926 39.0 35.0 40.0 29.0 41.0 56-57 35.60318200831975 38.5 35.0 40.0 28.0 41.0 58-59 35.25135259757067 38.0 34.5 40.0 27.5 41.0 60-61 34.860858990695604 38.0 34.0 40.0 26.5 41.0 62-63 34.449020035475186 37.5 34.0 40.0 26.0 41.0 64-65 34.035211946770225 37.0 33.5 39.5 26.0 41.0 66-67 33.53989037928427 36.0 33.0 39.0 24.5 40.5 68-69 33.023591640848814 35.5 32.0 39.0 22.5 40.0 70-71 32.450253547005204 35.0 31.5 38.0 21.5 40.0 72-73 31.988012835789654 35.0 31.0 37.5 20.5 39.5 74-75 31.43452305051388 35.0 31.0 37.0 18.5 39.0 76-77 30.185901150818182 33.5 29.5 35.5 18.0 38.0 78-79 30.876845687062698 34.0 31.0 36.0 18.0 38.0 80-81 30.69122140212857 34.0 31.0 36.0 17.5 37.5 82-83 30.23441335944579 34.0 30.0 35.0 10.5 37.0 84-85 29.728323042833747 34.0 30.0 35.0 7.0 36.5 86-87 29.201429384249685 34.0 29.0 35.0 2.0 36.0 88-89 28.60126769105578 34.0 29.0 35.0 2.0 35.5 90-91 28.081124195674562 33.0 27.5 35.0 2.0 35.0 92-93 27.46635427283645 33.0 26.5 35.0 2.0 35.0 94-95 26.742909580497617 32.5 25.0 35.0 2.0 35.0 96-97 25.950500073430284 32.0 24.5 34.0 2.0 35.0 98-99 24.947912718506394 32.0 18.5 34.0 2.0 35.0 100-101 21.40184759976772 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.045030773387594536 1101 2 0.10275017118209462 1101 3 0.13747584180888595 1101 4 0.07724778655435927 1101 5 0.012162164927168817 1101 6 0.008127088162588336 1101 7 0.06289517274433365 1101 8 0.112869534027773 1101 9 0.10976031243360751 1101 10-11 0.11305396714170257 1101 12-13 0.13048379706952318 1101 14-15 0.18020950993984286 1101 16-17 0.1422069226339744 1101 18-19 0.20804043052482513 1101 20-21 0.1943745582689118 1101 22-23 0.12177626117554752 1101 24-25 0.15425871384959322 1101 26-27 0.2222667953174735 1101 28-29 0.13636749737558063 1101 30-31 0.23211817473671204 1101 32-33 0.18117951979939306 1101 34-35 0.0904956179827181 1101 36-37 0.12686841851416375 1101 38-39 0.2722449815378951 1101 40-41 0.32486819068508765 1101 42-43 0.37284390114648147 1101 44-45 0.29103315032638477 1101 46-47 0.3053187055679558 1101 48-49 0.3403684574242831 1101 50-51 0.43163242281718084 1101 52-53 0.38286293874735833 1101 54-55 0.3823653533375122 1101 56-57 0.35162117683434957 1101 58-59 0.4171573257774597 1101 60-61 0.4757463733168521 1101 62-63 0.4762406904734462 1101 64-65 0.538885574248944 1101 66-67 0.5585881336668237 1101 68-69 0.44285666590174344 1101 70-71 0.5458455437137602 1101 72-73 0.6035478657725761 1101 74-75 0.6243146335659553 1101 76-77 0.6713244452716545 1101 78-79 0.908869162232623 1101 80-81 1.08184365627406 1101 82-83 1.108698715138484 1101 84-85 1.1352383137768065 1101 86-87 1.333326282604677 1101 88-89 1.5931127048006744 1101 90-91 1.6617831595767178 1101 92-93 1.8749980508761261 1101 94-95 1.9489426015692892 1101 96-97 2.0718037287526734 1101 98-99 2.3238254219871273 1101 100-101 2.69586516947415 1102 1 0.018924654370493954 1102 2 0.04852305799613532 1102 3 0.19621431838451997 1102 4 0.08431515149646884 1102 5 0.0015907153279073327 1102 6 0.015115560899509717 1102 7 0.07367343640631674 1102 8 0.043906204077806876 1102 9 -0.007065277020892324 1102 10-11 0.08889177323948871 1102 12-13 0.11688235731325136 1102 14-15 0.14380446359199084 1102 16-17 0.22009820282150372 1102 18-19 0.22912445178230456 1102 20-21 0.17348437886591483 1102 22-23 0.15257863726051113 1102 24-25 0.04064864360375253 1102 26-27 0.13512553644144276 1102 28-29 0.04809972134684415 1102 30-31 0.03193502833554618 1102 32-33 0.08919851534806611 1102 34-35 0.013679072010539528 1102 36-37 0.22934192741178094 1102 38-39 0.268815235576092 1102 40-41 0.2344256371826745 1102 42-43 0.18699790402578742 1102 44-45 0.3212480706955603 1102 46-47 0.2856052378213576 1102 48-49 0.2905423114885295 1102 50-51 0.4890797146172119 1102 52-53 0.3625977550423656 1102 54-55 0.300522473782614 1102 56-57 0.38150993882504025 1102 58-59 0.2831111034168785 1102 60-61 0.40373568633518175 1102 62-63 0.32295631266555347 1102 64-65 0.34570943231074835 1102 66-67 0.4710913303895694 1102 68-69 0.5127078243728818 1102 70-71 0.6036508155132907 1102 72-73 0.5889864826069058 1102 74-75 0.7124944103698461 1102 76-77 0.6583922655647427 1102 78-79 0.8461738444585265 1102 80-81 0.9419633818139026 1102 82-83 0.959489516836836 1102 84-85 1.0762363160567396 1102 86-87 1.3125742439824428 1102 88-89 1.418615642608728 1102 90-91 1.4126524298979461 1102 92-93 1.3968126457222958 1102 94-95 1.4192376213532292 1102 96-97 1.5979910167298748 1102 98-99 1.8665085935384944 1102 100-101 2.1762979964677776 1103 1 0.2882897629761132 1103 2 0.2710181361874149 1103 3 0.34265701925760084 1103 4 0.21281941038047592 1103 5 0.13455724702861716 1103 6 0.1496942904149492 1103 7 0.13906024483110002 1103 8 0.1694600093784473 1103 9 0.17535706742677348 1103 10-11 0.27296629149122253 1103 12-13 0.22477354241962644 1103 14-15 0.3951316915015042 1103 16-17 0.35272957532629334 1103 18-19 0.38743381831951496 1103 20-21 0.3323512788720535 1103 22-23 0.4380526284307038 1103 24-25 0.3914087513258906 1103 26-27 0.3839234997963672 1103 28-29 0.38872472526298196 1103 30-31 0.41048521574649044 1103 32-33 0.49336341191803257 1103 34-35 0.5024187506236402 1103 36-37 0.466590464027405 1103 38-39 0.48207479503818007 1103 40-41 0.5597479200998876 1103 42-43 0.4970227292404843 1103 44-45 0.5683429817145225 1103 46-47 0.4187276633488537 1103 48-49 0.45086119206057873 1103 50-51 0.46496554619924524 1103 52-53 0.5318274590821943 1103 54-55 0.5746194111706941 1103 56-57 0.5906425099829207 1103 58-59 0.7094183120025619 1103 60-61 0.692200044419792 1103 62-63 0.6923810093054499 1103 64-65 0.8275910357113432 1103 66-67 0.7530592769144775 1103 68-69 1.0116688245997096 1103 70-71 1.0249702988580864 1103 72-73 1.0235580064189307 1103 74-75 0.8289985414309378 1103 76-77 0.5811681155557835 1103 78-79 0.9397458557520544 1103 80-81 1.0616647148063763 1103 82-83 1.09542486100003 1103 84-85 1.148400683790726 1103 86-87 1.2448390654877706 1103 88-89 1.2775260274684825 1103 90-91 1.3204244389357775 1103 92-93 1.6019740597444674 1103 94-95 1.5198039896411188 1103 96-97 1.7051063042249055 1103 98-99 2.003259435352625 1103 100-101 2.2361660506396284 1104 1 0.15021436038458091 1104 2 0.09978446011503195 1104 3 0.12310959164565105 1104 4 0.08727612515976801 1104 5 0.04855534651058235 1104 6 0.04336099859778386 1104 7 0.08506915696904827 1104 8 0.0382407717075921 1104 9 0.06178543127448677 1104 10-11 0.10757359464691518 1104 12-13 0.09110565794528469 1104 14-15 0.08024503064709165 1104 16-17 0.04616448489022673 1104 18-19 0.12227361203027698 1104 20-21 0.07101494253571872 1104 22-23 0.08295271284692518 1104 24-25 0.09128766249512665 1104 26-27 0.1939202476963402 1104 28-29 0.17186062614082687 1104 30-31 0.22993796342505135 1104 32-33 0.272925462447823 1104 34-35 0.22271796689059187 1104 36-37 0.22169199412893192 1104 38-39 0.1819956836687524 1104 40-41 0.2785729663442922 1104 42-43 0.2868149408068916 1104 44-45 0.27360827004432764 1104 46-47 0.35344210128909737 1104 48-49 0.17250587822139352 1104 50-51 0.19036179498259287 1104 52-53 0.3045852399044904 1104 54-55 0.28766909680102515 1104 56-57 0.3551717858025185 1104 58-59 0.27558055719706687 1104 60-61 0.3137882224595856 1104 62-63 0.3395700185912247 1104 64-65 0.22791220941960688 1104 66-67 0.31090341137151967 1104 68-69 0.4856402614743729 1104 70-71 0.568811605602626 1104 72-73 0.48751954860627933 1104 74-75 0.5646330238396082 1104 76-77 0.577278281020785 1104 78-79 0.6506554981133128 1104 80-81 0.726532116770553 1104 82-83 0.6935080824703483 1104 84-85 0.94283428790175 1104 86-87 0.9124559911692103 1104 88-89 1.021540087611573 1104 90-91 1.0101942778991635 1104 92-93 1.2657098000931093 1104 94-95 1.5731127131111329 1104 96-97 1.7776131428607762 1104 98-99 2.114202867263799 1104 100-101 2.4118323851769325 1105 1 0.12485186406278359 1105 2 0.07513354640925485 1105 3 0.15437745069306175 1105 4 0.02253860907902805 1105 5 0.04604886991889856 1105 6 0.04027261018779882 1105 7 0.056783813121697335 1105 8 0.04337201800984758 1105 9 0.055394476164380535 1105 10-11 0.07281786906888499 1105 12-13 0.03487356476157544 1105 14-15 0.0581115803755381 1105 16-17 0.09963740410384503 1105 18-19 0.11359057574990317 1105 20-21 0.06507410055969132 1105 22-23 0.05263176329189889 1105 24-25 0.0842492219215174 1105 26-27 0.05695075585648368 1105 28-29 0.05061530358275945 1105 30-31 0.11917039741801005 1105 32-33 0.026934834875902425 1105 34-35 0.12594841995150574 1105 36-37 0.1337855721501029 1105 38-39 0.10228140758268722 1105 40-41 0.2512564622481577 1105 42-43 0.23371865750372933 1105 44-45 0.23817387895164188 1105 46-47 0.22086990861409106 1105 48-49 0.22483034669271973 1105 50-51 0.3215744418892683 1105 52-53 0.28402516628136 1105 54-55 0.39713965084708747 1105 56-57 0.3575029940286356 1105 58-59 0.4601811381361358 1105 60-61 0.4101480426818327 1105 62-63 0.46536055013901034 1105 64-65 0.5067049022052572 1105 66-67 0.4732246298219067 1105 68-69 0.6136743236392306 1105 70-71 0.7140933603568413 1105 72-73 0.733800220135997 1105 74-75 0.6204440994801566 1105 76-77 0.655155626516251 1105 78-79 0.7910944003253739 1105 80-81 0.9880627428764015 1105 82-83 0.9657635605187274 1105 84-85 1.171319878896604 1105 86-87 1.102037216157857 1105 88-89 1.1951060571305092 1105 90-91 1.3484136294722227 1105 92-93 1.4004928668413505 1105 94-95 1.5555711550535278 1105 96-97 1.6659890808244597 1105 98-99 2.1205061881911185 1105 100-101 2.530135014410863 1106 1 0.07623028708684387 1106 2 0.06903012994408897 1106 3 0.1653465781173722 1106 4 0.04277915736149396 1106 5 0.046041679789645684 1106 6 5.527476563855771E-4 1106 7 -0.006598179550728389 1106 8 -0.024666335811730278 1106 9 0.030906233614231837 1106 10-11 0.05685686899882114 1106 12-13 0.0374491359462823 1106 14-15 0.038403686901155254 1106 16-17 0.06035300422631451 1106 18-19 0.05203169887484904 1106 20-21 0.04068940423145051 1106 22-23 0.029546097583747155 1106 24-25 0.08896481803089529 1106 26-27 0.03564452173015553 1106 28-29 -0.008913490460336959 1106 30-31 0.10411455937583725 1106 32-33 0.1522026462891759 1106 34-35 0.053720812564002074 1106 36-37 0.10275656934244637 1106 38-39 0.18195978641253419 1106 40-41 0.11212202672931681 1106 42-43 0.01783929048257704 1106 44-45 0.1530509419543975 1106 46-47 -0.09072358120877766 1106 48-49 0.13374955154468893 1106 50-51 0.2750945201580066 1106 52-53 0.22265962863776423 1106 54-55 0.2557621040155098 1106 56-57 0.43202918541215496 1106 58-59 0.3706234381013047 1106 60-61 0.5315576390965333 1106 62-63 0.5385939395609256 1106 64-65 0.413453189658604 1106 66-67 0.42945375650044326 1106 68-69 0.1259956133945792 1106 70-71 0.3098261273309433 1106 72-73 0.5179800880750562 1106 74-75 0.6658546984998601 1106 76-77 0.6775494512988196 1106 78-79 0.872096139166171 1106 80-81 0.9427088288292111 1106 82-83 0.7734541060505542 1106 84-85 0.8273787500028327 1106 86-87 1.0342500570264193 1106 88-89 1.1643945068901544 1106 90-91 1.060833541047007 1106 92-93 0.9834124890552367 1106 94-95 1.2165139214574339 1106 96-97 1.3618877056871561 1106 98-99 1.795968709976794 1106 100-101 2.234531285175077 1107 1 0.0860744398974802 1107 2 0.1442816993790359 1107 3 0.1921817119477609 1107 4 0.10843256642528587 1107 5 0.05933497756173978 1107 6 0.05203247332867278 1107 7 0.0038383599689311154 1107 8 0.0426160969230196 1107 9 0.014784818365313868 1107 10-11 0.07425343522441352 1107 12-13 0.03646723468885682 1107 14-15 0.11702627562630141 1107 16-17 0.11596670762816785 1107 18-19 0.07482530103474261 1107 20-21 0.06242950319488472 1107 22-23 0.04897369258917905 1107 24-25 0.0761265903602748 1107 26-27 0.1452963676440575 1107 28-29 0.16489878453057116 1107 30-31 0.1435894243902638 1107 32-33 0.14462000094440697 1107 34-35 0.17859387823761352 1107 36-37 0.27573850051356885 1107 38-39 0.2615001528808065 1107 40-41 0.29484993649687397 1107 42-43 0.30235858529576376 1107 44-45 0.34350446227168163 1107 46-47 0.469554670290961 1107 48-49 0.4419892162821313 1107 50-51 0.5374762456883673 1107 52-53 0.4647246221951846 1107 54-55 0.47224682750314884 1107 56-57 0.4525207056740683 1107 58-59 0.5265058715361661 1107 60-61 0.5501126451381424 1107 62-63 0.6388241636622638 1107 64-65 0.6574530267244185 1107 66-67 0.753582766646602 1107 68-69 0.8193797215192404 1107 70-71 0.9288582250481809 1107 72-73 0.7301539770436847 1107 74-75 0.8514237732086833 1107 76-77 0.7973554864194128 1107 78-79 0.9019397478020643 1107 80-81 0.9281592352577306 1107 82-83 1.1363562026507985 1107 84-85 1.1364393707502778 1107 86-87 1.2953761232190715 1107 88-89 1.4860573075824952 1107 90-91 1.490584242189108 1107 92-93 1.6423906721035237 1107 94-95 1.7220981566834865 1107 96-97 1.692990187792244 1107 98-99 1.936075724643736 1107 100-101 2.566519801496977 1108 1 0.0868928121091983 1108 2 0.07772173723730447 1108 3 0.1795316525986621 1108 4 0.10292688670703143 1108 5 0.045045240555481314 1108 6 0.08383263798085494 1108 7 0.06265067407524327 1108 8 0.06526359458132447 1108 9 0.03336489857947811 1108 10-11 0.09755308851618594 1108 12-13 0.06554926941443284 1108 14-15 0.09856939122894914 1108 16-17 0.0986757836399903 1108 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-2.510135663807006 2316 96-97 -2.6636416043585065 2316 98-99 -3.1200902058953695 2316 100-101 -4.094963944705931 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 26.0 5 50.0 6 97.0 7 226.0 8 616.0 9 1338.0 10 2303.0 11 3817.0 12 6121.0 13 9062.0 14 12415.0 15 16180.0 16 21334.0 17 29995.0 18 30486.0 19 22050.0 20 19544.0 21 19981.0 22 20847.0 23 22904.0 24 26305.0 25 31005.0 26 36275.0 27 43533.0 28 52608.0 29 64290.0 30 78481.0 31 97048.0 32 122271.0 33 161175.0 34 222877.0 35 332767.0 36 524978.0 37 777986.0 38 560124.0 39 40266.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.14575896868118 19.36144086341117 15.272238750143268 23.220561417764383 2 15.340152677395343 20.096494177778077 40.46918439640421 24.094168748422376 3 19.024868872047495 24.092394051076873 30.11750800018293 26.765229076692705 4 12.841538864711769 17.216988460449354 37.436704834792074 32.5047678400468 5 13.472643238659233 37.28911436902351 34.7254943517109 14.512748040606358 6 31.704321372530224 36.87288447856931 17.265644855342085 14.15714929355838 7 28.221823363376725 32.85455615130332 20.795954973311538 18.127665512008416 8 25.822955125395914 35.44910513147847 19.726211711044893 19.001728032080717 9 25.899550301797003 16.936922359419114 20.61755309185469 36.54597424692919 10-11 24.68433038762103 26.746138461082975 27.437526363817305 21.132004787478685 12-13 25.510750867899063 24.020585103061823 27.973772636973866 22.49489139206525 14-15 22.25641151914508 25.590131790505886 26.39678032384954 25.75667636649949 16-17 21.764543968350797 28.855246105920827 27.171185294470273 22.209024631258103 18-19 21.669819332186 27.27234486496596 29.618830866749974 21.439004936098062 20-21 22.13382251680322 26.437892489269068 30.00116667677982 21.42711831714789 22-23 22.027810291320364 26.26636462049734 29.762774744320435 21.94305034386186 24-25 21.97017098981677 26.43848384075072 29.755490761924154 21.83585440750836 26-27 21.542728252809795 26.916191269781002 29.633657307869242 21.907423169539964 28-29 21.581792404531935 27.07135904592542 29.66876107228448 21.678087477258167 30-31 21.835713955937415 26.57602932522522 29.602428330325036 21.98582838851233 32-33 21.56681742610445 26.69349331608079 29.540192264819893 22.19949699299487 34-35 22.204556442167274 26.798599064225094 29.257287671771042 21.739556821836583 36-37 22.21738349744037 26.436973003873344 29.311043603490038 22.03459989519625 38-39 21.751373957629813 26.758019999844606 29.326938512051786 22.163667530473795 40-41 22.193825492493414 26.52053712480253 29.285355321227556 22.0002820614765 42-43 22.00987196584231 26.498419198365646 29.199569188126024 22.29213964766602 44-45 21.9407879355459 26.77551982459507 29.176285447549798 22.107406792309238 46-47 22.122586732099727 27.06663557414007 28.59297864890269 22.217799044857518 48-49 22.140925901579372 26.89756678255789 28.702711520543616 22.25879579531912 50-51 22.265879775423514 27.17058309508797 28.397176642192832 22.16636048729568 52-53 22.451434147691312 27.100919593398487 28.246505319371906 22.201140939538295 54-55 22.149153470467688 27.22623475002484 28.350861332843213 22.273750446664256 56-57 22.166946757034285 27.27605302106262 28.37986702816059 22.17713319374251 58-59 22.255268293154803 26.949500776660834 28.24511292874272 22.55011800144164 60-61 22.073011688166645 27.336673312034154 28.317766406099082 22.27254859370012 62-63 21.97073693226738 27.3925887883534 28.348926642705823 22.287747636673398 64-65 21.731055206383072 27.240100029342802 28.33273094117692 22.69611382309721 66-67 21.795998112206462 27.204966304057876 28.246110822861066 22.7529247608746 68-69 21.939509470250208 27.034333623488465 28.408229657469885 22.61792724879144 70-71 21.966999462683784 27.188947994649702 28.054809771592243 22.78924277107427 72-73 22.028616412210944 27.23828259315313 27.848164345478203 22.884936649157723 74-75 21.864079810072056 27.564102000381663 27.841979199735945 22.72983898981034 76-77 22.002190303743422 27.21267726231965 28.00414140943349 22.780991024503436 78-79 21.969403168612057 27.40302438970053 28.034231703767283 22.59334073792013 80-81 21.98784426542346 27.634480215419266 27.790867897404993 22.586807621752282 82-83 22.14700964539739 27.53461367990788 27.74662540071901 22.571751273975718 84-85 22.09329322310251 27.416917941901918 27.74512453734185 22.744664297653717 86-87 22.094261587469465 27.429406114067067 27.66497067608143 22.811361622382034 88-89 22.05226420370769 27.650599548923932 27.51149235591977 22.785643891448608 90-91 22.061765762068436 27.582927204637564 27.561762097749376 22.793544935544627 92-93 22.090356395359063 27.17741793643628 27.68794446822107 23.044281199983587 94-95 21.885274842722037 27.24529382615008 27.69815821760971 23.171273113518172 96-97 21.979325143123475 27.131975529036552 27.703296896572947 23.185402431267022 98-99 21.929738034974303 27.26114175436673 27.41796930020178 23.39115091045719 100-101 22.04674713720724 26.976406867683256 27.5477122355177 23.429133759591807 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 987.0 1 683.5 2 548.0 3 714.5 4 553.5 5 522.5 6 1097.5 7 2606.5 8 4354.5 9 5048.5 10 4277.0 11 3398.5 12 3442.0 13 3743.0 14 4070.0 15 4294.0 16 4468.0 17 4740.5 18 5029.0 19 5422.0 20 6166.5 21 7347.0 22 8742.5 23 10723.5 24 13424.0 25 16849.5 26 21687.0 27 27111.0 28 31816.0 29 37159.0 30 43476.5 31 50359.5 32 58715.0 33 64712.5 34 68535.0 35 72830.5 36 79289.5 37 86281.0 38 90619.5 39 93774.0 40 97270.0 41 104319.5 42 110474.5 43 117333.5 44 124680.0 45 129311.5 46 129956.0 47 130643.0 48 131342.5 49 126601.0 50 123763.5 51 119960.0 52 114520.5 53 111350.0 54 109987.0 55 103318.0 56 92782.5 57 84486.5 58 77538.5 59 68619.0 60 55505.0 61 45358.0 62 37720.5 63 28388.5 64 21629.5 65 17320.0 66 12545.5 67 9864.0 68 7591.0 69 5093.0 70 3730.5 71 2599.0 72 1910.0 73 1513.5 74 1156.0 75 923.0 76 601.5 77 273.5 78 138.0 79 79.5 80 35.0 81 12.0 82 10.0 83 8.0 84 4.5 85 2.0 86 1.5 87 1.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.012194410562939134 3 0.006654161533142269 4 2.931348693014215E-5 5 3.517618431617058E-4 6 1.758809215808529E-4 7 0.0 8 1.172539477205686E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.86269738602843E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001626898524622889 26-27 0.02169198032830519 28-29 2.784781258363504E-4 30-31 4.3970230395213223E-5 32-33 0.0033563942535012764 34-35 0.015712028994556194 36-37 0.007665476832232173 38-39 0.0211789943070277 40-41 0.023304222109463008 42-43 0.018452840022524484 44-45 0.009394972561110559 46-47 0.002213168263225732 48-49 3.0779161276649257E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.6382138237127935E-4 68-69 1.6122417811578182E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4656743465071075E-4 82-83 9.087180948344066E-4 84-85 0.003898693761708906 86-87 5.86269738602843E-4 88-89 2.931348693014215E-5 90-91 0.0036348723793376263 92-93 5.569562516727009E-4 94-95 7.328371732535537E-5 96-97 2.6382138237127935E-4 98-99 2.491646389062083E-4 100-101 0.00259424359331758 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3411399.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.8511894237469 #Duplication Level Percentage of deduplicated Percentage of total 1 67.60058382078498 30.31966590101907 2 16.157899591554 14.494020305413418 3 6.5136525399112175 8.764351917240845 4 3.171137659084996 5.689171833455939 5 1.8664715676751185 4.185673491791729 6 1.1463669928497777 3.08495538872619 7 0.7383505872794788 2.318113143584467 8 0.5239642543549505 1.8800336018676949 9 0.36912270439071393 1.490003310171028 >10 1.7164827230182442 13.504118728833017 >50 0.11552564820596059 3.589745691632211 >100 0.07243083311718379 6.204485218150432 >500 0.004990507467686783 1.5592129236771646 >1k 0.002823576590120113 1.923938335526842 >5k 6.566457186325845E-5 0.22730545377944716 >10k+ 1.313291437265169E-4 0.7652047551305515 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14299 0.4191535496141026 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11683 0.3424694678048507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7718 0.22624149212683708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.4656743465071076E-5 8.794046079042646E-5 14-15 0.0 0.0 0.0 5.86269738602843E-5 1.4656743465071075E-4 16-17 0.0 0.0 0.0 8.794046079042645E-5 1.758809215808529E-4 18-19 0.0 0.0 0.0 1.172539477205686E-4 2.0519440851099504E-4 20-21 0.0 0.0 0.0 1.6122417811578182E-4 2.345078954411372E-4 22-23 0.0 0.0 0.0 2.784781258363504E-4 2.345078954411372E-4 24-25 0.0 0.0 0.0 6.888669428583404E-4 2.345078954411372E-4 26-27 0.0 0.0 0.0 0.0012165097076008993 2.345078954411372E-4 28-29 0.0 0.0 0.0 0.004103888170219901 2.345078954411372E-4 30-31 0.0 0.0 0.0 0.013337636553214679 2.345078954411372E-4 32-33 0.0 0.0 0.0 0.03239140305780708 2.345078954411372E-4 34-35 0.0 0.0 0.0 0.06154366580983345 2.345078954411372E-4 36-37 0.0 0.0 0.0 0.10892891743240823 2.345078954411372E-4 38-39 0.0 0.0 0.0 0.17555847322462134 2.6382138237127935E-4 40-41 0.0 0.0 0.0 0.24831454778523415 2.6382138237127935E-4 42-43 0.0 0.0 0.0 0.32137841395861344 2.784781258363504E-4 44-45 0.0 0.0 0.0 0.411194937912569 2.931348693014215E-4 46-47 0.0 0.0 0.0 0.5183210758987735 2.931348693014215E-4 48-49 0.0 0.0 0.0 0.6263705887232774 3.517618431617058E-4 50-51 0.0 0.0 0.0 0.7387145273830472 3.8107533009184793E-4 52-53 0.0 0.0 0.0 0.8498859265656113 4.397023039521323E-4 54-55 0.0 0.0 0.0 0.9714636136083759 4.397023039521323E-4 56-57 0.0 0.0 0.0 1.1089438673107426 4.397023039521323E-4 58-59 0.0 0.0 0.0 1.2522868183991376 4.5435904741720335E-4 60-61 0.0 0.0 0.0 1.4052592499440846 4.690157908822744E-4 62-63 0.0 0.0 0.0 1.55900848889268 4.983292778124166E-4 64-65 0.0 0.0 0.0 1.7194998298352084 4.983292778124166E-4 66-67 0.0 0.0 0.0 1.903368676604525 4.983292778124166E-4 68-69 0.0 0.0 0.0 2.102128774734354 4.983292778124166E-4 70-71 0.0 0.0 0.0 2.308788857591856 5.129860212774876E-4 72-73 0.0 0.0 0.0 2.517720735686444 5.422995082076298E-4 74-75 0.0 0.0 0.0 2.73439137433059 5.569562516727009E-4 76-77 0.0 0.0 0.0 2.9661438020002935 5.569562516727009E-4 78-79 0.0 0.0 0.0 3.2218746619788536 5.569562516727009E-4 80-81 0.0 0.0 0.0 3.487953182843754 5.569562516727009E-4 82-83 0.0 0.0 0.0 3.7659916063761525 5.86269738602843E-4 84-85 0.0 0.0 0.0 4.047430394392447 5.86269738602843E-4 86-87 0.0 0.0 0.0 4.3347611932817 6.009264820679141E-4 88-89 0.0 0.0 0.0 4.644648720363699 6.155832255329851E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7095 0.0 49.21305 1 GTATCAA 12065 0.0 41.38288 1 ATCAACG 14820 0.0 33.366425 3 TCAACGC 15165 0.0 32.88588 4 TATCAAC 15265 0.0 32.48995 2 CAACGCA 15410 0.0 32.30139 5 AACGCAG 15885 0.0 31.455101 6 ACGCAGA 17845 0.0 27.760698 7 CGCAGAG 17895 0.0 27.497337 8 GCAGAGT 19470 0.0 24.93145 9 GAGTACT 12955 0.0 23.134201 12-13 GTACATG 9770 0.0 22.56154 1 GTGGTAT 3540 0.0 22.142467 1 GTACTTT 13610 0.0 22.003387 14-15 ACATGGG 9230 0.0 21.614975 3 CAGAGTA 19015 0.0 21.406569 10-11 TACATGG 9860 0.0 21.392 2 TAAGGTG 1620 0.0 21.109604 5 AGAGTAC 17890 0.0 20.86771 10-11 GGACCGA 1575 0.0 20.808039 6 >>END_MODULE