FastQCFastQC Report
Thu 26 May 2016
SRR938189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3452456
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154020.44611719888682144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117840.34132223553319724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76510.2216103550631782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74000.047.7580951
GTATCAA127150.041.9174461
ATCAACG157150.033.477943
TATCAAC158700.033.3006972
TCAACGC160300.032.8497244
CAACGCA161550.032.477875
AACGCAG166300.031.6073686
ACGCAGA186100.028.0507977
CGCAGAG188200.027.7622558
GCAGAGT203850.025.373249
GAGTACT132700.023.40776612-13
GTACATG104650.023.3200041
TACATGG103650.022.9258882
GTACTTT138350.022.02741214-15
ACATGGG103550.021.8006273
CAGAGTA198450.021.36760110-11
AGTACTT138100.020.97806212-13
AGAGTAC185700.020.94032510-11
GGACCGA14200.020.7505116
AGGACCG16100.020.6632025
Produced by FastQC (version 0.11.5)